A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba
2015
Authors
Aleksić, Jelena M.Banović Đeri, Bojana
Miljuš-Đukić, Jovanka
Jovanović, Živko
Mikić, Aleksandar
Cupina, Branko
Zlatković, Bojan
Anđelković, Snežana
Spanu, Ilaria
Jelić, Mihailo
Maksimović, Vesna R.
Article (Published version)
B'Y-NC
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Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important food and feed grain crop legume, they have not yet been used for comprehensive molecular characterization of this crop. We report a three-step procedure for rapid and cost-effective delineation and utilization of informative genomic nuclear SSRs for paralleled genotyping in faba bean suitable also for other species: (i) pre-selection of loci generating PCR products of expected lengths which are potentially polymorphic (achieved by PCR amplification in bulked samples); (ii) exclusion of loci burdened with persistent null alleles and multilocus amplification products (based on PCR amplification of pre-selected loci in individual genotypes), and (iii) multiplexing. We demonstrate also that genomic SSRs are promising molecular tools for molecular characterization of faba bean required also for crop improvement.
Keywords:
Type-it kit / multiplexing / genomic SSRs / faba beanSource:
Czech Journal of Genetics and Plant Breeding, 2015, 51, 1, 36-39Publisher:
- Czech Academy Agricultural Sciences, Prague
Funding / projects:
- The Role of Transcription Factors and Small RNAs in Abiotic Stress Response in Plants and Genetic Diversity of Plant Species Important for Agriculture and Biotechnology (RS-MESTD-Basic Research (BR or ON)-173005)
- Republic of Serbia "Pavle Savic" FABAGRALE [680-00-132/2012-09/15]
- Republic of France "Pavle Savic" FABAGRALE [680-00-132/2012-09/15]
DOI: 10.17221/153/2014-CJGPB
ISSN: 1212-1975
WoS: 000352167400006
Scopus: 2-s2.0-84925437364
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Aleksić, Jelena M. AU - Banović Đeri, Bojana AU - Miljuš-Đukić, Jovanka AU - Jovanović, Živko AU - Mikić, Aleksandar AU - Cupina, Branko AU - Zlatković, Bojan AU - Anđelković, Snežana AU - Spanu, Ilaria AU - Jelić, Mihailo AU - Maksimović, Vesna R. PY - 2015 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/834 AB - Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important food and feed grain crop legume, they have not yet been used for comprehensive molecular characterization of this crop. We report a three-step procedure for rapid and cost-effective delineation and utilization of informative genomic nuclear SSRs for paralleled genotyping in faba bean suitable also for other species: (i) pre-selection of loci generating PCR products of expected lengths which are potentially polymorphic (achieved by PCR amplification in bulked samples); (ii) exclusion of loci burdened with persistent null alleles and multilocus amplification products (based on PCR amplification of pre-selected loci in individual genotypes), and (iii) multiplexing. We demonstrate also that genomic SSRs are promising molecular tools for molecular characterization of faba bean required also for crop improvement. PB - Czech Academy Agricultural Sciences, Prague T2 - Czech Journal of Genetics and Plant Breeding T1 - A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba EP - 39 IS - 1 SP - 36 VL - 51 DO - 10.17221/153/2014-CJGPB ER -
@article{ author = "Aleksić, Jelena M. and Banović Đeri, Bojana and Miljuš-Đukić, Jovanka and Jovanović, Živko and Mikić, Aleksandar and Cupina, Branko and Zlatković, Bojan and Anđelković, Snežana and Spanu, Ilaria and Jelić, Mihailo and Maksimović, Vesna R.", year = "2015", abstract = "Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important food and feed grain crop legume, they have not yet been used for comprehensive molecular characterization of this crop. We report a three-step procedure for rapid and cost-effective delineation and utilization of informative genomic nuclear SSRs for paralleled genotyping in faba bean suitable also for other species: (i) pre-selection of loci generating PCR products of expected lengths which are potentially polymorphic (achieved by PCR amplification in bulked samples); (ii) exclusion of loci burdened with persistent null alleles and multilocus amplification products (based on PCR amplification of pre-selected loci in individual genotypes), and (iii) multiplexing. We demonstrate also that genomic SSRs are promising molecular tools for molecular characterization of faba bean required also for crop improvement.", publisher = "Czech Academy Agricultural Sciences, Prague", journal = "Czech Journal of Genetics and Plant Breeding", title = "A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba", pages = "39-36", number = "1", volume = "51", doi = "10.17221/153/2014-CJGPB" }
Aleksić, J. M., Banović Đeri, B., Miljuš-Đukić, J., Jovanović, Ž., Mikić, A., Cupina, B., Zlatković, B., Anđelković, S., Spanu, I., Jelić, M.,& Maksimović, V. R.. (2015). A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba. in Czech Journal of Genetics and Plant Breeding Czech Academy Agricultural Sciences, Prague., 51(1), 36-39. https://doi.org/10.17221/153/2014-CJGPB
Aleksić JM, Banović Đeri B, Miljuš-Đukić J, Jovanović Ž, Mikić A, Cupina B, Zlatković B, Anđelković S, Spanu I, Jelić M, Maksimović VR. A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba. in Czech Journal of Genetics and Plant Breeding. 2015;51(1):36-39. doi:10.17221/153/2014-CJGPB .
Aleksić, Jelena M., Banović Đeri, Bojana, Miljuš-Đukić, Jovanka, Jovanović, Živko, Mikić, Aleksandar, Cupina, Branko, Zlatković, Bojan, Anđelković, Snežana, Spanu, Ilaria, Jelić, Mihailo, Maksimović, Vesna R., "A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba" in Czech Journal of Genetics and Plant Breeding, 51, no. 1 (2015):36-39, https://doi.org/10.17221/153/2014-CJGPB . .