SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines
2015
Аутори
Petrović, IsidoraMilivojević, Milena
Popović, Jelena
Schwirtlich, Marija
Ranković, Branislava
Stevanović, Milena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Although there is much evidence showing functional relationship between Hedgehog pathway, in particular Sonic hedgehog, and SOX transcription factors during embryonic development, scarce data are available regarding their crosstalk in cancer cells. SOX18 protein plays an important role in promoting tumor angiogenesis and therefore emerged as a promising potential target in antiangiogenic tumor therapy. Recently it became evident that expression of SOX18 gene in tumors is not restricted to endothelium of accompanying blood and lymphatic vessels, but in tumor cells as well. In this paper we have identified human SOX18 gene as a novel target gene of Hedgehog signaling in cervical carcinoma cell lines. We have presented data showing that expression of SOX18 gene is regulated by GLI1 and GLI2 transcription factors, final effectors of Hedgehog signaling, and that modulation of Hedgehog signaling activity in considerably influence SOX18 expression. We consider important that Hedgehog pathway ...inhibitors reduced SOX18 expression, thus showing, for the first time, possibility for manipulationwith SOX18 gene expression. In addition, we analyzed the role of SOX18 in malignant potential of cervical carcinoma cell line, and showed that its overexpression has no influence on cells proliferation and viability, but substantially promotes migration and invasion of cells in vitro. Pro-migratory effect of SOX18 suggests its role in promoting malignant spreading, possibly in response to Hedgehog activation.
Извор:
PLoS One, 2015, 10, 11Издавач:
- Public Library Science, San Francisco
Финансирање / пројекти:
- Проучавање сигналних путева и епигенетичких механизама укључених у контролу експресије хуманих SOX гена: даље расветљавање њихове улоге у одређивању судбине и диференцијацији ћелија (RS-MESTD-Basic Research (BR or ON)-173051)
DOI: 10.1371/journal.pone.0143591
ISSN: 1932-6203
PubMed: 26588701
WoS: 000365251500065
Scopus: 2-s2.0-84957545982
Колекције
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Petrović, Isidora AU - Milivojević, Milena AU - Popović, Jelena AU - Schwirtlich, Marija AU - Ranković, Branislava AU - Stevanović, Milena PY - 2015 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/836 AB - Although there is much evidence showing functional relationship between Hedgehog pathway, in particular Sonic hedgehog, and SOX transcription factors during embryonic development, scarce data are available regarding their crosstalk in cancer cells. SOX18 protein plays an important role in promoting tumor angiogenesis and therefore emerged as a promising potential target in antiangiogenic tumor therapy. Recently it became evident that expression of SOX18 gene in tumors is not restricted to endothelium of accompanying blood and lymphatic vessels, but in tumor cells as well. In this paper we have identified human SOX18 gene as a novel target gene of Hedgehog signaling in cervical carcinoma cell lines. We have presented data showing that expression of SOX18 gene is regulated by GLI1 and GLI2 transcription factors, final effectors of Hedgehog signaling, and that modulation of Hedgehog signaling activity in considerably influence SOX18 expression. We consider important that Hedgehog pathway inhibitors reduced SOX18 expression, thus showing, for the first time, possibility for manipulationwith SOX18 gene expression. In addition, we analyzed the role of SOX18 in malignant potential of cervical carcinoma cell line, and showed that its overexpression has no influence on cells proliferation and viability, but substantially promotes migration and invasion of cells in vitro. Pro-migratory effect of SOX18 suggests its role in promoting malignant spreading, possibly in response to Hedgehog activation. PB - Public Library Science, San Francisco T2 - PLoS One T1 - SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines IS - 11 VL - 10 DO - 10.1371/journal.pone.0143591 ER -
@article{ author = "Petrović, Isidora and Milivojević, Milena and Popović, Jelena and Schwirtlich, Marija and Ranković, Branislava and Stevanović, Milena", year = "2015", abstract = "Although there is much evidence showing functional relationship between Hedgehog pathway, in particular Sonic hedgehog, and SOX transcription factors during embryonic development, scarce data are available regarding their crosstalk in cancer cells. SOX18 protein plays an important role in promoting tumor angiogenesis and therefore emerged as a promising potential target in antiangiogenic tumor therapy. Recently it became evident that expression of SOX18 gene in tumors is not restricted to endothelium of accompanying blood and lymphatic vessels, but in tumor cells as well. In this paper we have identified human SOX18 gene as a novel target gene of Hedgehog signaling in cervical carcinoma cell lines. We have presented data showing that expression of SOX18 gene is regulated by GLI1 and GLI2 transcription factors, final effectors of Hedgehog signaling, and that modulation of Hedgehog signaling activity in considerably influence SOX18 expression. We consider important that Hedgehog pathway inhibitors reduced SOX18 expression, thus showing, for the first time, possibility for manipulationwith SOX18 gene expression. In addition, we analyzed the role of SOX18 in malignant potential of cervical carcinoma cell line, and showed that its overexpression has no influence on cells proliferation and viability, but substantially promotes migration and invasion of cells in vitro. Pro-migratory effect of SOX18 suggests its role in promoting malignant spreading, possibly in response to Hedgehog activation.", publisher = "Public Library Science, San Francisco", journal = "PLoS One", title = "SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines", number = "11", volume = "10", doi = "10.1371/journal.pone.0143591" }
Petrović, I., Milivojević, M., Popović, J., Schwirtlich, M., Ranković, B.,& Stevanović, M.. (2015). SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines. in PLoS One Public Library Science, San Francisco., 10(11). https://doi.org/10.1371/journal.pone.0143591
Petrović I, Milivojević M, Popović J, Schwirtlich M, Ranković B, Stevanović M. SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines. in PLoS One. 2015;10(11). doi:10.1371/journal.pone.0143591 .
Petrović, Isidora, Milivojević, Milena, Popović, Jelena, Schwirtlich, Marija, Ranković, Branislava, Stevanović, Milena, "SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines" in PLoS One, 10, no. 11 (2015), https://doi.org/10.1371/journal.pone.0143591 . .