Construction of improved vectors for gene cloning in Micromonospora melanosporea
Samo za registrovane korisnike
1994
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Two vectors for Micromonospora melanosporea have been constructed with the Streptomyces plasmid pIJ702 along with the sgm gene (sisomicin-gentamicin resistance gene from M. zionensis) as the second antibiotic resistance marker. These plasmids, containing sgm gene as the second selectable marker, may be an attractive alternative to pIJ702, which is incapable of conferring melanin production in M. melanosporea and consequently is not useful for insertional inactivation in this bacterium. The constructions remove restriction site for M. melanosporea restriction endonuclease and provide additional unique sites for the insertional inactivation of selectable markers, which enhance the use of these plasmids as general cloning vectors in both M. melanosporea and S. lividans. On the other side, in S. lividans, plasmid pMK33-1 facilitates isolation and studies of promoters based on detection of extremely convenient phenotype of melanin production. This has been proved by shotgun cloning of chrom...osomal DNA fragments of M. melanosporea and chromogenic identification of S. lividans transformants which were capable of producing a melanin.
Izvor:
Current Microbiology, 1994, 28, 5, 283-287Izdavač:
- Springer-Verlag
Institucija/grupa
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kojić, Milorad AU - Topisirović, Ljubiša AU - Vasiljević, Branka PY - 1994 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/86 AB - Two vectors for Micromonospora melanosporea have been constructed with the Streptomyces plasmid pIJ702 along with the sgm gene (sisomicin-gentamicin resistance gene from M. zionensis) as the second antibiotic resistance marker. These plasmids, containing sgm gene as the second selectable marker, may be an attractive alternative to pIJ702, which is incapable of conferring melanin production in M. melanosporea and consequently is not useful for insertional inactivation in this bacterium. The constructions remove restriction site for M. melanosporea restriction endonuclease and provide additional unique sites for the insertional inactivation of selectable markers, which enhance the use of these plasmids as general cloning vectors in both M. melanosporea and S. lividans. On the other side, in S. lividans, plasmid pMK33-1 facilitates isolation and studies of promoters based on detection of extremely convenient phenotype of melanin production. This has been proved by shotgun cloning of chromosomal DNA fragments of M. melanosporea and chromogenic identification of S. lividans transformants which were capable of producing a melanin. PB - Springer-Verlag T2 - Current Microbiology T1 - Construction of improved vectors for gene cloning in Micromonospora melanosporea EP - 287 IS - 5 SP - 283 VL - 28 DO - 10.1007/BF01573207 ER -
@article{ author = "Kojić, Milorad and Topisirović, Ljubiša and Vasiljević, Branka", year = "1994", abstract = "Two vectors for Micromonospora melanosporea have been constructed with the Streptomyces plasmid pIJ702 along with the sgm gene (sisomicin-gentamicin resistance gene from M. zionensis) as the second antibiotic resistance marker. These plasmids, containing sgm gene as the second selectable marker, may be an attractive alternative to pIJ702, which is incapable of conferring melanin production in M. melanosporea and consequently is not useful for insertional inactivation in this bacterium. The constructions remove restriction site for M. melanosporea restriction endonuclease and provide additional unique sites for the insertional inactivation of selectable markers, which enhance the use of these plasmids as general cloning vectors in both M. melanosporea and S. lividans. On the other side, in S. lividans, plasmid pMK33-1 facilitates isolation and studies of promoters based on detection of extremely convenient phenotype of melanin production. This has been proved by shotgun cloning of chromosomal DNA fragments of M. melanosporea and chromogenic identification of S. lividans transformants which were capable of producing a melanin.", publisher = "Springer-Verlag", journal = "Current Microbiology", title = "Construction of improved vectors for gene cloning in Micromonospora melanosporea", pages = "287-283", number = "5", volume = "28", doi = "10.1007/BF01573207" }
Kojić, M., Topisirović, L.,& Vasiljević, B.. (1994). Construction of improved vectors for gene cloning in Micromonospora melanosporea. in Current Microbiology Springer-Verlag., 28(5), 283-287. https://doi.org/10.1007/BF01573207
Kojić M, Topisirović L, Vasiljević B. Construction of improved vectors for gene cloning in Micromonospora melanosporea. in Current Microbiology. 1994;28(5):283-287. doi:10.1007/BF01573207 .
Kojić, Milorad , Topisirović, Ljubiša, Vasiljević, Branka, "Construction of improved vectors for gene cloning in Micromonospora melanosporea" in Current Microbiology, 28, no. 5 (1994):283-287, https://doi.org/10.1007/BF01573207 . .