An improved HPLC-DAD method for simultaneously measuring phenolics in the leaves of Tilia platyphyllos and Ailanthus altissima
Апстракт
Phenolic compounds are one of the most important groups of secondary metabolites in plants, with various physiological functions. Their diverse chemical structure and susceptibility to auto-oxidation, and their ability to act as both antioxidants and prooxidants in the presence of metal ions, are some of the main reasons why it is difficult to measure phenolic groups in plant tissues accurately. We present an optimized extraction and hydrolysis procedure which preserves the original chemical structure of phenolics. The presented HPLC method was improved to enable the simultaneous separation and quantification of 39 compounds from different phenolic subclasses (benzoic acids, hydroxycinnamic acids, flavones, flavonols, flavanones, flavanols, isoflavones, anthocyanidins; aglycones and glycosides). Recovery after extraction and complete hydrolysis of glycosides was more than 95% and 84%, respectively. The method was applied to the analysis and comparison of phenolic profiles in the leaves... of two species, Ailanthus altissima (Mill.) Swingle and Tilia platyphyllos Scop. from an urban forest park and busy traffic area. The presence of cyanidin glycosides in A. altissima leaves was reported for the first time. Results indicated higher accumulation of phenolics, with two hydroxyl groups in the ortho- position, than flavonoids, with a monohydroxy substitution in the leaves of both species from a busy traffic area.
Кључне речи:
Urban environment / Phenolic profiles / Phenolic acids / HPLC method development / Flavonoid glycosides / AnthocyaninsИзвор:
Botanica Serbica, 2015, 39, 2, 177-186Издавач:
- Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
Финансирање / пројекти:
- Модификације антиоксидативног метаболизма биљака са циљем повећања толеранције на абиотски стрес и идентификација нових биомаркера са применом у ремедијацији и мониторингу деградираних станишта (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43010)
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Vidović, Marija AU - Morina, Filis AU - Milić, S. AU - Jovanović, S.V. PY - 2015 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/867 AB - Phenolic compounds are one of the most important groups of secondary metabolites in plants, with various physiological functions. Their diverse chemical structure and susceptibility to auto-oxidation, and their ability to act as both antioxidants and prooxidants in the presence of metal ions, are some of the main reasons why it is difficult to measure phenolic groups in plant tissues accurately. We present an optimized extraction and hydrolysis procedure which preserves the original chemical structure of phenolics. The presented HPLC method was improved to enable the simultaneous separation and quantification of 39 compounds from different phenolic subclasses (benzoic acids, hydroxycinnamic acids, flavones, flavonols, flavanones, flavanols, isoflavones, anthocyanidins; aglycones and glycosides). Recovery after extraction and complete hydrolysis of glycosides was more than 95% and 84%, respectively. The method was applied to the analysis and comparison of phenolic profiles in the leaves of two species, Ailanthus altissima (Mill.) Swingle and Tilia platyphyllos Scop. from an urban forest park and busy traffic area. The presence of cyanidin glycosides in A. altissima leaves was reported for the first time. Results indicated higher accumulation of phenolics, with two hydroxyl groups in the ortho- position, than flavonoids, with a monohydroxy substitution in the leaves of both species from a busy traffic area. PB - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd T2 - Botanica Serbica T1 - An improved HPLC-DAD method for simultaneously measuring phenolics in the leaves of Tilia platyphyllos and Ailanthus altissima EP - 186 IS - 2 SP - 177 VL - 39 UR - https://hdl.handle.net/21.15107/rcub_imagine_867 ER -
@article{ author = "Vidović, Marija and Morina, Filis and Milić, S. and Jovanović, S.V.", year = "2015", abstract = "Phenolic compounds are one of the most important groups of secondary metabolites in plants, with various physiological functions. Their diverse chemical structure and susceptibility to auto-oxidation, and their ability to act as both antioxidants and prooxidants in the presence of metal ions, are some of the main reasons why it is difficult to measure phenolic groups in plant tissues accurately. We present an optimized extraction and hydrolysis procedure which preserves the original chemical structure of phenolics. The presented HPLC method was improved to enable the simultaneous separation and quantification of 39 compounds from different phenolic subclasses (benzoic acids, hydroxycinnamic acids, flavones, flavonols, flavanones, flavanols, isoflavones, anthocyanidins; aglycones and glycosides). Recovery after extraction and complete hydrolysis of glycosides was more than 95% and 84%, respectively. The method was applied to the analysis and comparison of phenolic profiles in the leaves of two species, Ailanthus altissima (Mill.) Swingle and Tilia platyphyllos Scop. from an urban forest park and busy traffic area. The presence of cyanidin glycosides in A. altissima leaves was reported for the first time. Results indicated higher accumulation of phenolics, with two hydroxyl groups in the ortho- position, than flavonoids, with a monohydroxy substitution in the leaves of both species from a busy traffic area.", publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd", journal = "Botanica Serbica", title = "An improved HPLC-DAD method for simultaneously measuring phenolics in the leaves of Tilia platyphyllos and Ailanthus altissima", pages = "186-177", number = "2", volume = "39", url = "https://hdl.handle.net/21.15107/rcub_imagine_867" }
Vidović, M., Morina, F., Milić, S.,& Jovanović, S.V.. (2015). An improved HPLC-DAD method for simultaneously measuring phenolics in the leaves of Tilia platyphyllos and Ailanthus altissima. in Botanica Serbica Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 39(2), 177-186. https://hdl.handle.net/21.15107/rcub_imagine_867
Vidović M, Morina F, Milić S, Jovanović S. An improved HPLC-DAD method for simultaneously measuring phenolics in the leaves of Tilia platyphyllos and Ailanthus altissima. in Botanica Serbica. 2015;39(2):177-186. https://hdl.handle.net/21.15107/rcub_imagine_867 .
Vidović, Marija, Morina, Filis, Milić, S., Jovanović, S.V., "An improved HPLC-DAD method for simultaneously measuring phenolics in the leaves of Tilia platyphyllos and Ailanthus altissima" in Botanica Serbica, 39, no. 2 (2015):177-186, https://hdl.handle.net/21.15107/rcub_imagine_867 .