In vitro and in vivo methodologies for studying the sigma 54-dependent transcription
Само за регистроване кориснике
2015
Аутори
Buck, M.Engl, C.
Joly, N.
Jovanović, Goran
Jovanović, Milija
Lawton, E.
McDonald, C.
Schumacher, J.
Waite, C.
Zhang, N.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Here we describe approaches and methods to assaying in vitro the major variant bacterial sigma factor, Sigma 54 (σ54), in a purified system. We include the complete transcription system, binding interactions between σ54 and its activators, as well as the self-assembly and the critical ATPase activity of the cognate activators which serve to remodel the closed promoter complexes. We also present in vivo methodologies that are used to study the impact of physiological processes, metabolic states, global signalling networks, and cellular architecture on the control of σ54-dependent gene expression.
Кључне речи:
σ54 / Transcription activation / RNA polymerase / Open and closed promoter complexes / Bacterial enhancer binding proteins / ATPase / AAA+ proteinsИзвор:
Methods in Molecular Biology, 2015, 1276, 53-79Издавач:
- Humana Press Inc.
Финансирање / пројекти:
- Интелигентни роботски системи за екстремно диверзификовану производњу (RS-MESTD-Technological Development (TD or TR)-35007)
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Buck, M. AU - Engl, C. AU - Joly, N. AU - Jovanović, Goran AU - Jovanović, Milija AU - Lawton, E. AU - McDonald, C. AU - Schumacher, J. AU - Waite, C. AU - Zhang, N. PY - 2015 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/869 AB - Here we describe approaches and methods to assaying in vitro the major variant bacterial sigma factor, Sigma 54 (σ54), in a purified system. We include the complete transcription system, binding interactions between σ54 and its activators, as well as the self-assembly and the critical ATPase activity of the cognate activators which serve to remodel the closed promoter complexes. We also present in vivo methodologies that are used to study the impact of physiological processes, metabolic states, global signalling networks, and cellular architecture on the control of σ54-dependent gene expression. PB - Humana Press Inc. T2 - Methods in Molecular Biology T1 - In vitro and in vivo methodologies for studying the sigma 54-dependent transcription EP - 79 SP - 53 VL - 1276 DO - 10.1007/978-1-4939-2392-2_4 ER -
@article{ author = "Buck, M. and Engl, C. and Joly, N. and Jovanović, Goran and Jovanović, Milija and Lawton, E. and McDonald, C. and Schumacher, J. and Waite, C. and Zhang, N.", year = "2015", abstract = "Here we describe approaches and methods to assaying in vitro the major variant bacterial sigma factor, Sigma 54 (σ54), in a purified system. We include the complete transcription system, binding interactions between σ54 and its activators, as well as the self-assembly and the critical ATPase activity of the cognate activators which serve to remodel the closed promoter complexes. We also present in vivo methodologies that are used to study the impact of physiological processes, metabolic states, global signalling networks, and cellular architecture on the control of σ54-dependent gene expression.", publisher = "Humana Press Inc.", journal = "Methods in Molecular Biology", title = "In vitro and in vivo methodologies for studying the sigma 54-dependent transcription", pages = "79-53", volume = "1276", doi = "10.1007/978-1-4939-2392-2_4" }
Buck, M., Engl, C., Joly, N., Jovanović, G., Jovanović, M., Lawton, E., McDonald, C., Schumacher, J., Waite, C.,& Zhang, N.. (2015). In vitro and in vivo methodologies for studying the sigma 54-dependent transcription. in Methods in Molecular Biology Humana Press Inc.., 1276, 53-79. https://doi.org/10.1007/978-1-4939-2392-2_4
Buck M, Engl C, Joly N, Jovanović G, Jovanović M, Lawton E, McDonald C, Schumacher J, Waite C, Zhang N. In vitro and in vivo methodologies for studying the sigma 54-dependent transcription. in Methods in Molecular Biology. 2015;1276:53-79. doi:10.1007/978-1-4939-2392-2_4 .
Buck, M., Engl, C., Joly, N., Jovanović, Goran, Jovanović, Milija, Lawton, E., McDonald, C., Schumacher, J., Waite, C., Zhang, N., "In vitro and in vivo methodologies for studying the sigma 54-dependent transcription" in Methods in Molecular Biology, 1276 (2015):53-79, https://doi.org/10.1007/978-1-4939-2392-2_4 . .