Mononucleotide repeats in the SMAD4 gene promoter in colon carcinoma tissue of Croatian patients
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2015
Authors
Nikolić, AleksandraCacev, Tamara
Aralica, Gorana
Hadzija, Marijana Popovic
Kapitanović, Sanja
Radojković, Dragica
Article (Published version)
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This study was aimed at the analysis of mononudeotide repeats -462T(15) and -4T(12) in the SMAD4 gene promoter in sporadic colon adenocarcinoma tissue of Croatian patients. The analysis has included 60 pairs of samples of colon tumor and adjacent normal tissue. The number of thymidines in the tracts -462T(15) and -4T(12) of the SMAD4 gene promoter was determined by PCR with fluorescently labeled primers followed by the analysis of obtained DNA fragments by capillary electrophoresis. In the normal colon tissue two haplotypes were present: -462T(15)/4T(12) in 51 patients (85%) and -462T(16)/-4T(12) in 9 patients (15%). Among the cases with haplotype -462T(15)/-4T(12) detected in normal colon tissue, in 5 cases (8%) malignant tissue displayed different haplotypes: 462T(10)/-4T(10), -462T(12)/- 4T(12), 462T(13)/-4T(11), -462T(14)/-4T(10) and -462T(15)/-4T(11). Haplotype -462T(14)/-4T(10) was previously found to be associated with significantly decreased SMAD4 gene promoter activity in comp...arison to the wild type, while the other detected haplotypes remain to be functionally characterized. This study has shown that functionally relevant somatic alterations of the SMAD4 gene promoter are found in some colon cancer tumors. Although not as frequent in colon as in pancreatic cancer, they may be of significance for certain cases and their role in colon tumorigenesis should be investigated further.
Keywords:
SMAD4 / Promoter / Mononucleotide repeat / Colon cancerSource:
Experimental and Molecular Pathology, 2015, 98, 2, 133-135Publisher:
- Academic Press Inc Elsevier Science, San Diego
Funding / projects:
- Complex diseases as a model system for phenotype modulation- structural and functional analysis of molecular biomarkers (RS-MESTD-Basic Research (BR or ON)-173008)
- Croatian Ministry of Science, Education and Sport [098-0982464-2508]
DOI: 10.1016/j.yexmp.2015.02.008
ISSN: 0014-4800
PubMed: 25742737
WoS: 000352927500001
Scopus: 2-s2.0-84924696766
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Nikolić, Aleksandra AU - Cacev, Tamara AU - Aralica, Gorana AU - Hadzija, Marijana Popovic AU - Kapitanović, Sanja AU - Radojković, Dragica PY - 2015 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/884 AB - This study was aimed at the analysis of mononudeotide repeats -462T(15) and -4T(12) in the SMAD4 gene promoter in sporadic colon adenocarcinoma tissue of Croatian patients. The analysis has included 60 pairs of samples of colon tumor and adjacent normal tissue. The number of thymidines in the tracts -462T(15) and -4T(12) of the SMAD4 gene promoter was determined by PCR with fluorescently labeled primers followed by the analysis of obtained DNA fragments by capillary electrophoresis. In the normal colon tissue two haplotypes were present: -462T(15)/4T(12) in 51 patients (85%) and -462T(16)/-4T(12) in 9 patients (15%). Among the cases with haplotype -462T(15)/-4T(12) detected in normal colon tissue, in 5 cases (8%) malignant tissue displayed different haplotypes: 462T(10)/-4T(10), -462T(12)/- 4T(12), 462T(13)/-4T(11), -462T(14)/-4T(10) and -462T(15)/-4T(11). Haplotype -462T(14)/-4T(10) was previously found to be associated with significantly decreased SMAD4 gene promoter activity in comparison to the wild type, while the other detected haplotypes remain to be functionally characterized. This study has shown that functionally relevant somatic alterations of the SMAD4 gene promoter are found in some colon cancer tumors. Although not as frequent in colon as in pancreatic cancer, they may be of significance for certain cases and their role in colon tumorigenesis should be investigated further. PB - Academic Press Inc Elsevier Science, San Diego T2 - Experimental and Molecular Pathology T1 - Mononucleotide repeats in the SMAD4 gene promoter in colon carcinoma tissue of Croatian patients EP - 135 IS - 2 SP - 133 VL - 98 DO - 10.1016/j.yexmp.2015.02.008 ER -
@article{ author = "Nikolić, Aleksandra and Cacev, Tamara and Aralica, Gorana and Hadzija, Marijana Popovic and Kapitanović, Sanja and Radojković, Dragica", year = "2015", abstract = "This study was aimed at the analysis of mononudeotide repeats -462T(15) and -4T(12) in the SMAD4 gene promoter in sporadic colon adenocarcinoma tissue of Croatian patients. The analysis has included 60 pairs of samples of colon tumor and adjacent normal tissue. The number of thymidines in the tracts -462T(15) and -4T(12) of the SMAD4 gene promoter was determined by PCR with fluorescently labeled primers followed by the analysis of obtained DNA fragments by capillary electrophoresis. In the normal colon tissue two haplotypes were present: -462T(15)/4T(12) in 51 patients (85%) and -462T(16)/-4T(12) in 9 patients (15%). Among the cases with haplotype -462T(15)/-4T(12) detected in normal colon tissue, in 5 cases (8%) malignant tissue displayed different haplotypes: 462T(10)/-4T(10), -462T(12)/- 4T(12), 462T(13)/-4T(11), -462T(14)/-4T(10) and -462T(15)/-4T(11). Haplotype -462T(14)/-4T(10) was previously found to be associated with significantly decreased SMAD4 gene promoter activity in comparison to the wild type, while the other detected haplotypes remain to be functionally characterized. This study has shown that functionally relevant somatic alterations of the SMAD4 gene promoter are found in some colon cancer tumors. Although not as frequent in colon as in pancreatic cancer, they may be of significance for certain cases and their role in colon tumorigenesis should be investigated further.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Experimental and Molecular Pathology", title = "Mononucleotide repeats in the SMAD4 gene promoter in colon carcinoma tissue of Croatian patients", pages = "135-133", number = "2", volume = "98", doi = "10.1016/j.yexmp.2015.02.008" }
Nikolić, A., Cacev, T., Aralica, G., Hadzija, M. P., Kapitanović, S.,& Radojković, D.. (2015). Mononucleotide repeats in the SMAD4 gene promoter in colon carcinoma tissue of Croatian patients. in Experimental and Molecular Pathology Academic Press Inc Elsevier Science, San Diego., 98(2), 133-135. https://doi.org/10.1016/j.yexmp.2015.02.008
Nikolić A, Cacev T, Aralica G, Hadzija MP, Kapitanović S, Radojković D. Mononucleotide repeats in the SMAD4 gene promoter in colon carcinoma tissue of Croatian patients. in Experimental and Molecular Pathology. 2015;98(2):133-135. doi:10.1016/j.yexmp.2015.02.008 .
Nikolić, Aleksandra, Cacev, Tamara, Aralica, Gorana, Hadzija, Marijana Popovic, Kapitanović, Sanja, Radojković, Dragica, "Mononucleotide repeats in the SMAD4 gene promoter in colon carcinoma tissue of Croatian patients" in Experimental and Molecular Pathology, 98, no. 2 (2015):133-135, https://doi.org/10.1016/j.yexmp.2015.02.008 . .