The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1
2015
Authors
Drakulić, DanijelaVicentić, Jelena Marjanovic
Schwirtlich, Marija
Tosić, Jelena
Krstić, Aleksandar
Lazić, Andrijana
Stevanović, Milena
Article (Published version)
Metadata
Show full item recordAbstract
The altered expression of the SOX2 transcription factor is associated with oncogenic or tumor suppressor functions in human cancers. This factor regulates the migration and invasion of different cancer cells. In this study we investigated the effect of constitutive SOX2 overexpression on the migration and adhesion capacity of embryonal teratocarcinoma NT2/D1 cells derived from a metastasis of a human testicular germ cell tumor. We detected that increased SOX2 expression changed the speed, mode and path of cell migration, but not the adhesion ability of NT2/D1 cells. Additionally, we demonstrated that SOX2 overexpression increased the expression of the tumor suppressor protein p53 and the HDM2 oncogene. Our results contribute to the better understanding of the effect of SOX2 on the behavior of tumor cells originating from a human testicular germ cell tumor. Considering that NT2/D1 cells resemble cancer stem cells in many features, our results could contribute to the elucidation of the r...ole of SOX2 in cancer stem cells behavior and the process of metastasis.
Keywords:
wound healing / SOX2 / p53 / migration / HDM2 / adhesionSource:
Anais da Academia Brasileira de Ciencias, 2015, 87, 1, 389-405Publisher:
- Acad Brasileira De Ciencias, Rio Janeiro
Funding / projects:
- Studying signal transduction pathways and epigenetic mechanisms that control human SOX genes expression: further insight into their roles in cell fate determination and differentiation (RS-MESTD-Basic Research (BR or ON)-173051)
- Serbian Academy of Sciences and Arts [F 24]
DOI: 10.1590/0001-3765201520140352
ISSN: 0001-3765
PubMed: 25761220
WoS: 000351560600033
Scopus: 2-s2.0-84925950061
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Drakulić, Danijela AU - Vicentić, Jelena Marjanovic AU - Schwirtlich, Marija AU - Tosić, Jelena AU - Krstić, Aleksandar AU - Lazić, Andrijana AU - Stevanović, Milena PY - 2015 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/889 AB - The altered expression of the SOX2 transcription factor is associated with oncogenic or tumor suppressor functions in human cancers. This factor regulates the migration and invasion of different cancer cells. In this study we investigated the effect of constitutive SOX2 overexpression on the migration and adhesion capacity of embryonal teratocarcinoma NT2/D1 cells derived from a metastasis of a human testicular germ cell tumor. We detected that increased SOX2 expression changed the speed, mode and path of cell migration, but not the adhesion ability of NT2/D1 cells. Additionally, we demonstrated that SOX2 overexpression increased the expression of the tumor suppressor protein p53 and the HDM2 oncogene. Our results contribute to the better understanding of the effect of SOX2 on the behavior of tumor cells originating from a human testicular germ cell tumor. Considering that NT2/D1 cells resemble cancer stem cells in many features, our results could contribute to the elucidation of the role of SOX2 in cancer stem cells behavior and the process of metastasis. PB - Acad Brasileira De Ciencias, Rio Janeiro T2 - Anais da Academia Brasileira de Ciencias T1 - The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1 EP - 405 IS - 1 SP - 389 VL - 87 DO - 10.1590/0001-3765201520140352 ER -
@article{ author = "Drakulić, Danijela and Vicentić, Jelena Marjanovic and Schwirtlich, Marija and Tosić, Jelena and Krstić, Aleksandar and Lazić, Andrijana and Stevanović, Milena", year = "2015", abstract = "The altered expression of the SOX2 transcription factor is associated with oncogenic or tumor suppressor functions in human cancers. This factor regulates the migration and invasion of different cancer cells. In this study we investigated the effect of constitutive SOX2 overexpression on the migration and adhesion capacity of embryonal teratocarcinoma NT2/D1 cells derived from a metastasis of a human testicular germ cell tumor. We detected that increased SOX2 expression changed the speed, mode and path of cell migration, but not the adhesion ability of NT2/D1 cells. Additionally, we demonstrated that SOX2 overexpression increased the expression of the tumor suppressor protein p53 and the HDM2 oncogene. Our results contribute to the better understanding of the effect of SOX2 on the behavior of tumor cells originating from a human testicular germ cell tumor. Considering that NT2/D1 cells resemble cancer stem cells in many features, our results could contribute to the elucidation of the role of SOX2 in cancer stem cells behavior and the process of metastasis.", publisher = "Acad Brasileira De Ciencias, Rio Janeiro", journal = "Anais da Academia Brasileira de Ciencias", title = "The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1", pages = "405-389", number = "1", volume = "87", doi = "10.1590/0001-3765201520140352" }
Drakulić, D., Vicentić, J. M., Schwirtlich, M., Tosić, J., Krstić, A., Lazić, A.,& Stevanović, M.. (2015). The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1. in Anais da Academia Brasileira de Ciencias Acad Brasileira De Ciencias, Rio Janeiro., 87(1), 389-405. https://doi.org/10.1590/0001-3765201520140352
Drakulić D, Vicentić JM, Schwirtlich M, Tosić J, Krstić A, Lazić A, Stevanović M. The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1. in Anais da Academia Brasileira de Ciencias. 2015;87(1):389-405. doi:10.1590/0001-3765201520140352 .
Drakulić, Danijela, Vicentić, Jelena Marjanovic, Schwirtlich, Marija, Tosić, Jelena, Krstić, Aleksandar, Lazić, Andrijana, Stevanović, Milena, "The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1" in Anais da Academia Brasileira de Ciencias, 87, no. 1 (2015):389-405, https://doi.org/10.1590/0001-3765201520140352 . .