Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned
Authorized Users Only
2015
Authors
Novović, KatarinaFilipić, B.
Veljović, Katarina
Begović, Jelena
Mirković, N.
Jovčić, Branko
Article (Published version)
Metadata
Show full item recordAbstract
New Delhi metallo-beta-lactamase-1 (NDM-1) will soon become the most commonly isolated and distributed metallo-beta-lactamase worldwide due to its rapid international dissemination and its ability to be expressed by numerous Gram-negative pathogens. NDM-positive bacteria pose a significant public health threat in the Indian subcontinent and the Balkans, which have been designated as endemic regions. Our study was focused on urban rivers, a lake and springheads as a potential source of NDM-1-producing strains in Serbia, but also as a source of other metallo-beta-lactamases and extended-spectrum beta-lactamase (ESBL) producing bacteria. A total of 69 beta-lactam resistant isolates, belonging to 12 bacterial genera, were collected from 8 out of 10 different locations in Belgrade, of which the most were from a popular recreational site, Ada Ciganlija Lake. Phenotypic tests revealed 7 (10.14%) ESBL-producing isolates and 39 (56.52%) isolates resistant to imipenem, of which 32 were positive ...for metallo-beta-lactamase (MBL) production. PCR and sequencing revealed the presence of genetic determinants for SHV (3 isolates), DHA-1 (1 isolate) and CMY-2 (1 isolate) beta-lactamases. However, we did not detect any NDM-1-producing strains (previously described cases of NDM-1 from Serbia were limited to Belgrade), so we propose that Serbian NDM-1 is in fact a transplant and a nosocomial, rather than an environmental, issue and that Serbia is not an endemic region for NDM-1.
Keywords:
New Delhi metallo-beta-lactamase (NDM-1) / Environment / Antibiotic resistanceSource:
Science of the Total Environment, 2015, 511, 393-398Publisher:
- Elsevier, Amsterdam
Funding / projects:
- Genes and molecular mechanisms promoting probiotic activity of lactic acid bacteria from Western Balkan (RS-MESTD-Basic Research (BR or ON)-173019)
DOI: 10.1016/j.scitotenv.2014.12.072
ISSN: 0048-9697
PubMed: 25569574
WoS: 000350513900040
Scopus: 2-s2.0-84920575864
Collections
Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Novović, Katarina AU - Filipić, B. AU - Veljović, Katarina AU - Begović, Jelena AU - Mirković, N. AU - Jovčić, Branko PY - 2015 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/893 AB - New Delhi metallo-beta-lactamase-1 (NDM-1) will soon become the most commonly isolated and distributed metallo-beta-lactamase worldwide due to its rapid international dissemination and its ability to be expressed by numerous Gram-negative pathogens. NDM-positive bacteria pose a significant public health threat in the Indian subcontinent and the Balkans, which have been designated as endemic regions. Our study was focused on urban rivers, a lake and springheads as a potential source of NDM-1-producing strains in Serbia, but also as a source of other metallo-beta-lactamases and extended-spectrum beta-lactamase (ESBL) producing bacteria. A total of 69 beta-lactam resistant isolates, belonging to 12 bacterial genera, were collected from 8 out of 10 different locations in Belgrade, of which the most were from a popular recreational site, Ada Ciganlija Lake. Phenotypic tests revealed 7 (10.14%) ESBL-producing isolates and 39 (56.52%) isolates resistant to imipenem, of which 32 were positive for metallo-beta-lactamase (MBL) production. PCR and sequencing revealed the presence of genetic determinants for SHV (3 isolates), DHA-1 (1 isolate) and CMY-2 (1 isolate) beta-lactamases. However, we did not detect any NDM-1-producing strains (previously described cases of NDM-1 from Serbia were limited to Belgrade), so we propose that Serbian NDM-1 is in fact a transplant and a nosocomial, rather than an environmental, issue and that Serbia is not an endemic region for NDM-1. PB - Elsevier, Amsterdam T2 - Science of the Total Environment T1 - Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned EP - 398 SP - 393 VL - 511 DO - 10.1016/j.scitotenv.2014.12.072 ER -
@article{ author = "Novović, Katarina and Filipić, B. and Veljović, Katarina and Begović, Jelena and Mirković, N. and Jovčić, Branko", year = "2015", abstract = "New Delhi metallo-beta-lactamase-1 (NDM-1) will soon become the most commonly isolated and distributed metallo-beta-lactamase worldwide due to its rapid international dissemination and its ability to be expressed by numerous Gram-negative pathogens. NDM-positive bacteria pose a significant public health threat in the Indian subcontinent and the Balkans, which have been designated as endemic regions. Our study was focused on urban rivers, a lake and springheads as a potential source of NDM-1-producing strains in Serbia, but also as a source of other metallo-beta-lactamases and extended-spectrum beta-lactamase (ESBL) producing bacteria. A total of 69 beta-lactam resistant isolates, belonging to 12 bacterial genera, were collected from 8 out of 10 different locations in Belgrade, of which the most were from a popular recreational site, Ada Ciganlija Lake. Phenotypic tests revealed 7 (10.14%) ESBL-producing isolates and 39 (56.52%) isolates resistant to imipenem, of which 32 were positive for metallo-beta-lactamase (MBL) production. PCR and sequencing revealed the presence of genetic determinants for SHV (3 isolates), DHA-1 (1 isolate) and CMY-2 (1 isolate) beta-lactamases. However, we did not detect any NDM-1-producing strains (previously described cases of NDM-1 from Serbia were limited to Belgrade), so we propose that Serbian NDM-1 is in fact a transplant and a nosocomial, rather than an environmental, issue and that Serbia is not an endemic region for NDM-1.", publisher = "Elsevier, Amsterdam", journal = "Science of the Total Environment", title = "Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned", pages = "398-393", volume = "511", doi = "10.1016/j.scitotenv.2014.12.072" }
Novović, K., Filipić, B., Veljović, K., Begović, J., Mirković, N.,& Jovčić, B.. (2015). Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned. in Science of the Total Environment Elsevier, Amsterdam., 511, 393-398. https://doi.org/10.1016/j.scitotenv.2014.12.072
Novović K, Filipić B, Veljović K, Begović J, Mirković N, Jovčić B. Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned. in Science of the Total Environment. 2015;511:393-398. doi:10.1016/j.scitotenv.2014.12.072 .
Novović, Katarina, Filipić, B., Veljović, Katarina, Begović, Jelena, Mirković, N., Jovčić, Branko, "Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned" in Science of the Total Environment, 511 (2015):393-398, https://doi.org/10.1016/j.scitotenv.2014.12.072 . .