Effects of DMSO, glycerol, betaine and their combinations in detecting single nucleotide polymorphisms of epidermal growth factor receptor (EGFR) gene promoter sequence in non-small-cell lung cancer (NSCLC) patients
Аутори
Jurisić, VladimirObradović, Jasmina
Tošić, Nataša
Pavlović, Sonja
Kulić, Milan
Đorđević, Nataša
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of the study was to examine the effects of frequently used polymerase chain reaction (PCR) additives DMSO, glycerol and betaine on amplification of GC-rich epidermal growth factor receptor (EGFR) gene promoter region, in order to detect the presence of -216G gt T and -191C gt A gene variations in non-small-cell lung cancer (NSCLC) patients. PCR products and restriction fragments were detected by electrophoresis on 8% polyacrylamide gel and 3% agarose gel. Our analysis shows that single used additives including DMSO in concentration of 7% and 10%, glycerol in concentration of 10%, 15% and 20%, as well as betaine in concentration of 1 M, 1.5 M and 2 M significantly enhanced the yield and specificity of PCR reaction. In addition, the combination of 10% DMSO with 15% glycerol has shown positive effects, whereas other analyzed combinations of additives failed to amplify the EGFR promoter region.
Кључне речи:
PCR / Optimization / Glycerol / EGFR / DMSO / Betaine / AdditivesИзвор:
Journal of Pharmaceutical and Biomedical Analysis, 2016, 128, 275-279Издавач:
- Elsevier, Amsterdam
Финансирање / пројекти:
- Молекулске, биохемијске и имунолошке анализе у дијагностици тумора (RS-MESTD-Basic Research (BR or ON)-175056)
Напомена:
- This is the peer reviewed version of the paper: Jurišić, V., Obradović, J., Tošić, N., Pavlović, S., Kulić, M., & Djordjević, N. (2016). Effects of DMSO, glycerol, betaine and their combinations in detecting single nucleotide polymorphisms of epidermal growth factor receptor (EGFR) gene promoter sequence in non-small-cell lung cancer (NSCLC) patients. Journal of Pharmaceutical and Biomedical Analysis, 128, 275–279. https://doi.org/10.1016/j.jpba.2016.05.010
- https://www.sciencedirect.com/science/article/abs/pii/S0731708516302473?via%3Dihub
Повезане информације:
- Друга верзија
https://doi.org/10.1016/j.jpba.2016.05.010
DOI: 10.1016/j.jpba.2016.05.010
ISSN: 0731-7085
PubMed: 27284695
WoS: 000381591900033
Scopus: 2-s2.0-84973129537
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Jurisić, Vladimir AU - Obradović, Jasmina AU - Tošić, Nataša AU - Pavlović, Sonja AU - Kulić, Milan AU - Đorđević, Nataša PY - 2016 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/903 AB - The aim of the study was to examine the effects of frequently used polymerase chain reaction (PCR) additives DMSO, glycerol and betaine on amplification of GC-rich epidermal growth factor receptor (EGFR) gene promoter region, in order to detect the presence of -216G gt T and -191C gt A gene variations in non-small-cell lung cancer (NSCLC) patients. PCR products and restriction fragments were detected by electrophoresis on 8% polyacrylamide gel and 3% agarose gel. Our analysis shows that single used additives including DMSO in concentration of 7% and 10%, glycerol in concentration of 10%, 15% and 20%, as well as betaine in concentration of 1 M, 1.5 M and 2 M significantly enhanced the yield and specificity of PCR reaction. In addition, the combination of 10% DMSO with 15% glycerol has shown positive effects, whereas other analyzed combinations of additives failed to amplify the EGFR promoter region. PB - Elsevier, Amsterdam T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - Effects of DMSO, glycerol, betaine and their combinations in detecting single nucleotide polymorphisms of epidermal growth factor receptor (EGFR) gene promoter sequence in non-small-cell lung cancer (NSCLC) patients EP - 279 SP - 275 VL - 128 DO - 10.1016/j.jpba.2016.05.010 ER -
@article{ author = "Jurisić, Vladimir and Obradović, Jasmina and Tošić, Nataša and Pavlović, Sonja and Kulić, Milan and Đorđević, Nataša", year = "2016", abstract = "The aim of the study was to examine the effects of frequently used polymerase chain reaction (PCR) additives DMSO, glycerol and betaine on amplification of GC-rich epidermal growth factor receptor (EGFR) gene promoter region, in order to detect the presence of -216G gt T and -191C gt A gene variations in non-small-cell lung cancer (NSCLC) patients. PCR products and restriction fragments were detected by electrophoresis on 8% polyacrylamide gel and 3% agarose gel. Our analysis shows that single used additives including DMSO in concentration of 7% and 10%, glycerol in concentration of 10%, 15% and 20%, as well as betaine in concentration of 1 M, 1.5 M and 2 M significantly enhanced the yield and specificity of PCR reaction. In addition, the combination of 10% DMSO with 15% glycerol has shown positive effects, whereas other analyzed combinations of additives failed to amplify the EGFR promoter region.", publisher = "Elsevier, Amsterdam", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "Effects of DMSO, glycerol, betaine and their combinations in detecting single nucleotide polymorphisms of epidermal growth factor receptor (EGFR) gene promoter sequence in non-small-cell lung cancer (NSCLC) patients", pages = "279-275", volume = "128", doi = "10.1016/j.jpba.2016.05.010" }
Jurisić, V., Obradović, J., Tošić, N., Pavlović, S., Kulić, M.,& Đorđević, N.. (2016). Effects of DMSO, glycerol, betaine and their combinations in detecting single nucleotide polymorphisms of epidermal growth factor receptor (EGFR) gene promoter sequence in non-small-cell lung cancer (NSCLC) patients. in Journal of Pharmaceutical and Biomedical Analysis Elsevier, Amsterdam., 128, 275-279. https://doi.org/10.1016/j.jpba.2016.05.010
Jurisić V, Obradović J, Tošić N, Pavlović S, Kulić M, Đorđević N. Effects of DMSO, glycerol, betaine and their combinations in detecting single nucleotide polymorphisms of epidermal growth factor receptor (EGFR) gene promoter sequence in non-small-cell lung cancer (NSCLC) patients. in Journal of Pharmaceutical and Biomedical Analysis. 2016;128:275-279. doi:10.1016/j.jpba.2016.05.010 .
Jurisić, Vladimir, Obradović, Jasmina, Tošić, Nataša, Pavlović, Sonja, Kulić, Milan, Đorđević, Nataša, "Effects of DMSO, glycerol, betaine and their combinations in detecting single nucleotide polymorphisms of epidermal growth factor receptor (EGFR) gene promoter sequence in non-small-cell lung cancer (NSCLC) patients" in Journal of Pharmaceutical and Biomedical Analysis, 128 (2016):275-279, https://doi.org/10.1016/j.jpba.2016.05.010 . .