Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia
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Kolundzić, Marina D.Grozdanić, Nada D.
Stanojković, Tatjana P.
Milenković, Marina T.
Dinić, Miroslav
Golić, Nataša
Kojić, Milan
Kundaković, Tatjana D.
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Cyclohexane, dichloromethane, and methanol extracts of the medicinal mushroom Laetiporus sulphureus were tested for their antimicrobial, cytotoxic, and radical scavenging activities. Total polyphenol content was also determined. Regarding the amount of the phenolic compounds in the methanol extract, there were 14.1 +/- 0.6 mg gallic acid/g dry extract and a concentration of 0.66 +/- 0.15 mg/mL resulted in the removal of 50% of the DPPH radicals (half-maximal inhibitory concentration [IC50]) . The average minimum inhibitory concentrations (MICs) against a select panel of microorganisms were between 125 and 250 mu g/mL, and the methanolic extract significantly inhibited the growth of Staphylococcus aureus (MIC, 31.25 mu g/mL). Also, cyclohexane and dichloromethane extracts inhibited the growth of Helicobacter pylori (MIC, 62.5 mu g/mL). Cyclohexane and dichloromethane extracts showed higher antiproliferative action compared with the methanol extract. The lowest IC50 was induced on HeLa c...ells by dichloromethane extract (IC50,67.20 +/- 5.49 mu g/mL), followed by cyclohexane extract (IC50 75.91 +/- 1.57 mu g/mL). The tested cyclohexane and dichloromethane extracts possessed moderate antibacterial and significant cytotoxic activity against both malignant (HeLa and N87) and healthy (MRC5) cell lines. Methanol extracts exhibited promising anti-Helicobacter activity without cytotoxicity against N87 and MRC5 cell lines.
Keywords:
medicinal mushrooms / Laetiporus sulphureus / Helicobacter pylori / cytotoxicity / antimicrobial activitySource:
International Journal of Medicinal Mushrooms, 2016, 18, 6, 469-476Publisher:
- Begell House Inc, Danbury
Funding / projects:
- Investigation on the medicinal plants: morphological, chemical and pharmacological characterisation (RS-MESTD-Basic Research (BR or ON)-173021)
- Plants and synthetic bioactive products of new generation (RS-MESTD-Technological Development (TD or TR)-34012)
DOI: 10.1615/IntJMedMushrooms.v18.i6.10
ISSN: 1521-9437
WoS: 000386997500001
Scopus: 2-s2.0-84986222835
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Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Kolundzić, Marina D. AU - Grozdanić, Nada D. AU - Stanojković, Tatjana P. AU - Milenković, Marina T. AU - Dinić, Miroslav AU - Golić, Nataša AU - Kojić, Milan AU - Kundaković, Tatjana D. PY - 2016 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/921 AB - Cyclohexane, dichloromethane, and methanol extracts of the medicinal mushroom Laetiporus sulphureus were tested for their antimicrobial, cytotoxic, and radical scavenging activities. Total polyphenol content was also determined. Regarding the amount of the phenolic compounds in the methanol extract, there were 14.1 +/- 0.6 mg gallic acid/g dry extract and a concentration of 0.66 +/- 0.15 mg/mL resulted in the removal of 50% of the DPPH radicals (half-maximal inhibitory concentration [IC50]) . The average minimum inhibitory concentrations (MICs) against a select panel of microorganisms were between 125 and 250 mu g/mL, and the methanolic extract significantly inhibited the growth of Staphylococcus aureus (MIC, 31.25 mu g/mL). Also, cyclohexane and dichloromethane extracts inhibited the growth of Helicobacter pylori (MIC, 62.5 mu g/mL). Cyclohexane and dichloromethane extracts showed higher antiproliferative action compared with the methanol extract. The lowest IC50 was induced on HeLa cells by dichloromethane extract (IC50,67.20 +/- 5.49 mu g/mL), followed by cyclohexane extract (IC50 75.91 +/- 1.57 mu g/mL). The tested cyclohexane and dichloromethane extracts possessed moderate antibacterial and significant cytotoxic activity against both malignant (HeLa and N87) and healthy (MRC5) cell lines. Methanol extracts exhibited promising anti-Helicobacter activity without cytotoxicity against N87 and MRC5 cell lines. PB - Begell House Inc, Danbury T2 - International Journal of Medicinal Mushrooms T1 - Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia EP - 476 IS - 6 SP - 469 VL - 18 DO - 10.1615/IntJMedMushrooms.v18.i6.10 ER -
@article{ author = "Kolundzić, Marina D. and Grozdanić, Nada D. and Stanojković, Tatjana P. and Milenković, Marina T. and Dinić, Miroslav and Golić, Nataša and Kojić, Milan and Kundaković, Tatjana D.", year = "2016", abstract = "Cyclohexane, dichloromethane, and methanol extracts of the medicinal mushroom Laetiporus sulphureus were tested for their antimicrobial, cytotoxic, and radical scavenging activities. Total polyphenol content was also determined. Regarding the amount of the phenolic compounds in the methanol extract, there were 14.1 +/- 0.6 mg gallic acid/g dry extract and a concentration of 0.66 +/- 0.15 mg/mL resulted in the removal of 50% of the DPPH radicals (half-maximal inhibitory concentration [IC50]) . The average minimum inhibitory concentrations (MICs) against a select panel of microorganisms were between 125 and 250 mu g/mL, and the methanolic extract significantly inhibited the growth of Staphylococcus aureus (MIC, 31.25 mu g/mL). Also, cyclohexane and dichloromethane extracts inhibited the growth of Helicobacter pylori (MIC, 62.5 mu g/mL). Cyclohexane and dichloromethane extracts showed higher antiproliferative action compared with the methanol extract. The lowest IC50 was induced on HeLa cells by dichloromethane extract (IC50,67.20 +/- 5.49 mu g/mL), followed by cyclohexane extract (IC50 75.91 +/- 1.57 mu g/mL). The tested cyclohexane and dichloromethane extracts possessed moderate antibacterial and significant cytotoxic activity against both malignant (HeLa and N87) and healthy (MRC5) cell lines. Methanol extracts exhibited promising anti-Helicobacter activity without cytotoxicity against N87 and MRC5 cell lines.", publisher = "Begell House Inc, Danbury", journal = "International Journal of Medicinal Mushrooms", title = "Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia", pages = "476-469", number = "6", volume = "18", doi = "10.1615/IntJMedMushrooms.v18.i6.10" }
Kolundzić, M. D., Grozdanić, N. D., Stanojković, T. P., Milenković, M. T., Dinić, M., Golić, N., Kojić, M.,& Kundaković, T. D.. (2016). Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia. in International Journal of Medicinal Mushrooms Begell House Inc, Danbury., 18(6), 469-476. https://doi.org/10.1615/IntJMedMushrooms.v18.i6.10
Kolundzić MD, Grozdanić ND, Stanojković TP, Milenković MT, Dinić M, Golić N, Kojić M, Kundaković TD. Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia. in International Journal of Medicinal Mushrooms. 2016;18(6):469-476. doi:10.1615/IntJMedMushrooms.v18.i6.10 .
Kolundzić, Marina D., Grozdanić, Nada D., Stanojković, Tatjana P., Milenković, Marina T., Dinić, Miroslav, Golić, Nataša, Kojić, Milan, Kundaković, Tatjana D., "Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia" in International Journal of Medicinal Mushrooms, 18, no. 6 (2016):469-476, https://doi.org/10.1615/IntJMedMushrooms.v18.i6.10 . .