Assessment of Deoxyribonuclease Activity in Serum Samples of Patients With Systemic Lupus Erythematosus: Fluorescence-Based Method Versus ELISA
2016
Аутори
Vancevska, AleksandraNikolić, Aleksandra
Bonaci-Nikolić, Branka
Skiljević, Dusan
Radojković, Dragica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
BackgroundWe report the improvement of previously described method for determining deoxyribonuclease (DNase) activity in serum samples that uses a fluorescently labeled DNA fragment as a substrate MethodsActivity of serum DNase was analyzed in 31 patients with systemic lupus erythematosus (SLE) and 13 healthy individuals by fluoresence-based method and ELISA test ResultsWe found a mean decrease in DNase activity between cases and controls of 12.46% measured by the fluoresence-based method and of 12.21% measured by ELISA method. High level of positive correlation between two methods for DNase activity was observed: P lt 0.001 and Pearson correlation coefficient 0.740. Decreased DNase activity was found in 25 of 31 SLE patients (81%) by fluoresence-based method and in 24 of 31 SLE patients (77%) by ELISA test. We also observed the significant positive correlation between titer of anti-dsDNA antibodies and DNase activity measured by both methods (P lt 0.05). ConclusionsThe key improve...ment is the use of internal control in the fluorescence-based method, which diminishes the influence of technical errors on the obtained results and increases reliability of the assay. This improved fluorescence-based method, with additional validation, may provide an alternative to more expensive and time-consuming conventional methods, such as ELISA.
Кључне речи:
systemic lupus erythematosus / fluorescence / ELISA / deoxyribonuclease activityИзвор:
Journal of Clinical Laboratory Analysis, 2016, 30, 6, 797-803Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Комплексне болести као модел систем за проучавање модулације фенотипа-структурна и функционална анализа молекуларних биомаркера (RS-MESTD-Basic Research (BR or ON)-173008)
- Клинички значај поремећаја урођене и стечене имуности код имуноинфламаторних и имунодефицитних обољења (RS-MESTD-Basic Research (BR or ON)-175065)
DOI: 10.1002/jcla.21939
ISSN: 0887-8013
PubMed: 27086765
WoS: 000386935200001
Scopus: 2-s2.0-84963568766
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Vancevska, Aleksandra AU - Nikolić, Aleksandra AU - Bonaci-Nikolić, Branka AU - Skiljević, Dusan AU - Radojković, Dragica PY - 2016 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/922 AB - BackgroundWe report the improvement of previously described method for determining deoxyribonuclease (DNase) activity in serum samples that uses a fluorescently labeled DNA fragment as a substrate MethodsActivity of serum DNase was analyzed in 31 patients with systemic lupus erythematosus (SLE) and 13 healthy individuals by fluoresence-based method and ELISA test ResultsWe found a mean decrease in DNase activity between cases and controls of 12.46% measured by the fluoresence-based method and of 12.21% measured by ELISA method. High level of positive correlation between two methods for DNase activity was observed: P lt 0.001 and Pearson correlation coefficient 0.740. Decreased DNase activity was found in 25 of 31 SLE patients (81%) by fluoresence-based method and in 24 of 31 SLE patients (77%) by ELISA test. We also observed the significant positive correlation between titer of anti-dsDNA antibodies and DNase activity measured by both methods (P lt 0.05). ConclusionsThe key improvement is the use of internal control in the fluorescence-based method, which diminishes the influence of technical errors on the obtained results and increases reliability of the assay. This improved fluorescence-based method, with additional validation, may provide an alternative to more expensive and time-consuming conventional methods, such as ELISA. PB - Wiley, Hoboken T2 - Journal of Clinical Laboratory Analysis T1 - Assessment of Deoxyribonuclease Activity in Serum Samples of Patients With Systemic Lupus Erythematosus: Fluorescence-Based Method Versus ELISA EP - 803 IS - 6 SP - 797 VL - 30 DO - 10.1002/jcla.21939 ER -
@article{ author = "Vancevska, Aleksandra and Nikolić, Aleksandra and Bonaci-Nikolić, Branka and Skiljević, Dusan and Radojković, Dragica", year = "2016", abstract = "BackgroundWe report the improvement of previously described method for determining deoxyribonuclease (DNase) activity in serum samples that uses a fluorescently labeled DNA fragment as a substrate MethodsActivity of serum DNase was analyzed in 31 patients with systemic lupus erythematosus (SLE) and 13 healthy individuals by fluoresence-based method and ELISA test ResultsWe found a mean decrease in DNase activity between cases and controls of 12.46% measured by the fluoresence-based method and of 12.21% measured by ELISA method. High level of positive correlation between two methods for DNase activity was observed: P lt 0.001 and Pearson correlation coefficient 0.740. Decreased DNase activity was found in 25 of 31 SLE patients (81%) by fluoresence-based method and in 24 of 31 SLE patients (77%) by ELISA test. We also observed the significant positive correlation between titer of anti-dsDNA antibodies and DNase activity measured by both methods (P lt 0.05). ConclusionsThe key improvement is the use of internal control in the fluorescence-based method, which diminishes the influence of technical errors on the obtained results and increases reliability of the assay. This improved fluorescence-based method, with additional validation, may provide an alternative to more expensive and time-consuming conventional methods, such as ELISA.", publisher = "Wiley, Hoboken", journal = "Journal of Clinical Laboratory Analysis", title = "Assessment of Deoxyribonuclease Activity in Serum Samples of Patients With Systemic Lupus Erythematosus: Fluorescence-Based Method Versus ELISA", pages = "803-797", number = "6", volume = "30", doi = "10.1002/jcla.21939" }
Vancevska, A., Nikolić, A., Bonaci-Nikolić, B., Skiljević, D.,& Radojković, D.. (2016). Assessment of Deoxyribonuclease Activity in Serum Samples of Patients With Systemic Lupus Erythematosus: Fluorescence-Based Method Versus ELISA. in Journal of Clinical Laboratory Analysis Wiley, Hoboken., 30(6), 797-803. https://doi.org/10.1002/jcla.21939
Vancevska A, Nikolić A, Bonaci-Nikolić B, Skiljević D, Radojković D. Assessment of Deoxyribonuclease Activity in Serum Samples of Patients With Systemic Lupus Erythematosus: Fluorescence-Based Method Versus ELISA. in Journal of Clinical Laboratory Analysis. 2016;30(6):797-803. doi:10.1002/jcla.21939 .
Vancevska, Aleksandra, Nikolić, Aleksandra, Bonaci-Nikolić, Branka, Skiljević, Dusan, Radojković, Dragica, "Assessment of Deoxyribonuclease Activity in Serum Samples of Patients With Systemic Lupus Erythematosus: Fluorescence-Based Method Versus ELISA" in Journal of Clinical Laboratory Analysis, 30, no. 6 (2016):797-803, https://doi.org/10.1002/jcla.21939 . .