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Assessment of Deoxyribonuclease Activity in Serum Samples of Patients With Systemic Lupus Erythematosus: Fluorescence-Based Method Versus ELISA
dc.creator | Vancevska, Aleksandra | |
dc.creator | Nikolić, Aleksandra | |
dc.creator | Bonaci-Nikolić, Branka | |
dc.creator | Skiljević, Dusan | |
dc.creator | Radojković, Dragica | |
dc.date.accessioned | 2022-11-15T14:41:43Z | |
dc.date.available | 2022-11-15T14:41:43Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0887-8013 | |
dc.identifier.uri | https://imagine.imgge.bg.ac.rs/handle/123456789/922 | |
dc.description.abstract | BackgroundWe report the improvement of previously described method for determining deoxyribonuclease (DNase) activity in serum samples that uses a fluorescently labeled DNA fragment as a substrate MethodsActivity of serum DNase was analyzed in 31 patients with systemic lupus erythematosus (SLE) and 13 healthy individuals by fluoresence-based method and ELISA test ResultsWe found a mean decrease in DNase activity between cases and controls of 12.46% measured by the fluoresence-based method and of 12.21% measured by ELISA method. High level of positive correlation between two methods for DNase activity was observed: P lt 0.001 and Pearson correlation coefficient 0.740. Decreased DNase activity was found in 25 of 31 SLE patients (81%) by fluoresence-based method and in 24 of 31 SLE patients (77%) by ELISA test. We also observed the significant positive correlation between titer of anti-dsDNA antibodies and DNase activity measured by both methods (P lt 0.05). ConclusionsThe key improvement is the use of internal control in the fluorescence-based method, which diminishes the influence of technical errors on the obtained results and increases reliability of the assay. This improved fluorescence-based method, with additional validation, may provide an alternative to more expensive and time-consuming conventional methods, such as ELISA. | en |
dc.publisher | Wiley, Hoboken | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173008/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175065/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Journal of Clinical Laboratory Analysis | |
dc.subject | systemic lupus erythematosus | en |
dc.subject | fluorescence | en |
dc.subject | ELISA | en |
dc.subject | deoxyribonuclease activity | en |
dc.title | Assessment of Deoxyribonuclease Activity in Serum Samples of Patients With Systemic Lupus Erythematosus: Fluorescence-Based Method Versus ELISA | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dc.citation.epage | 803 | |
dc.citation.issue | 6 | |
dc.citation.other | 30(6): 797-803 | |
dc.citation.rank | M23 | |
dc.citation.spage | 797 | |
dc.citation.volume | 30 | |
dc.identifier.doi | 10.1002/jcla.21939 | |
dc.identifier.fulltext | https://imagine.imgge.bg.ac.rs/bitstream/id/824/919.pdf | |
dc.identifier.pmid | 27086765 | |
dc.identifier.scopus | 2-s2.0-84963568766 | |
dc.identifier.wos | 000386935200001 | |
dc.type.version | publishedVersion |