Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles
Само за регистроване кориснике
2016
Аутори
Jasnić, JovanaKrause, Sabine
Savić, Slobodan
Kojić, Ana
Kovcić, Vlado
Bošković, Srđan
Nestorović, Aleksandra
Rakićević, Ljiljana
Schreiber-Katz, Olivia
Vogel, Johannes G.
Schoser, Benedikt G.
Walter, Maggie C.
Valle, Giorgio
Radojković, Dragica
Faulkner, Georgine
Kojić, Snežana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Four human Ankrd2 transcripts, reported in the Ensembl database, code for distinct protein isoforms (360, 333, 327 and 300 aa), and so far, their existence, specific expression and localization patterns have not been studied in detail. Ankrd2 is preferentially expressed in the slow fibers of skeletal muscle. It is found in both the nuclei and the cytoplasm of skeletal muscle cells, and its localization is prone to change during differentiation and upon stress. Ankrd2 has also been detected in the heart, in ventricular cardiomyocytes and in the intercalated disks (ICDs). The main objective of this study was to distinguish between the Ankrd2 isoforms and to determine the contribution of each one to the general profile of Ankrd2 expression in striated muscles. We demonstrated that the known expression and localization pattern of Ankrd2 in striated muscle can be attributed to the isoform of 333 aa which is dominant in both tissues, while the designated cardiac and canonical isoform of 360 ...aa was less expressed in both tissues. The 360 aa isoform has a distinct nuclear localization in human skeletal muscle, as well as in primary myoblasts and myotubes. In contrast to the isoform of 333 aa, it was not preferentially expressed in slow fibers and not localized to the ICDs of human cardiomyocytes. Regulation of the expression of both isoforms is achieved at the transcriptional level. Our results set the stage for investigation of the specific functions and interactions of the Ankrd2 isoforms in healthy and diseased human striated muscles.
Кључне речи:
Skeletal muscle / Isoforms / Human / Heart / Ankrd2Извор:
Histochemistry and Cell Biology, 2016, 146, 5, 569-584Издавач:
- Springer, New York
Финансирање / пројекти:
- German Academic Exchange Service
- Fondazione Cariparo, Italy
- German network for mitochondrial disorders (mito-NET) - German Ministry of Education and Research (BMBF, Bonn, Germany) [01GM1113A]
- Комплексне болести као модел систем за проучавање модулације фенотипа-структурна и функционална анализа молекуларних биомаркера (RS-MESTD-Basic Research (BR or ON)-173008)
- 50753561
DOI: 10.1007/s00418-016-1465-0
ISSN: 0948-6143
PubMed: 27393496
WoS: 000386695800006
Scopus: 2-s2.0-84978161931
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Jasnić, Jovana AU - Krause, Sabine AU - Savić, Slobodan AU - Kojić, Ana AU - Kovcić, Vlado AU - Bošković, Srđan AU - Nestorović, Aleksandra AU - Rakićević, Ljiljana AU - Schreiber-Katz, Olivia AU - Vogel, Johannes G. AU - Schoser, Benedikt G. AU - Walter, Maggie C. AU - Valle, Giorgio AU - Radojković, Dragica AU - Faulkner, Georgine AU - Kojić, Snežana PY - 2016 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/933 AB - Four human Ankrd2 transcripts, reported in the Ensembl database, code for distinct protein isoforms (360, 333, 327 and 300 aa), and so far, their existence, specific expression and localization patterns have not been studied in detail. Ankrd2 is preferentially expressed in the slow fibers of skeletal muscle. It is found in both the nuclei and the cytoplasm of skeletal muscle cells, and its localization is prone to change during differentiation and upon stress. Ankrd2 has also been detected in the heart, in ventricular cardiomyocytes and in the intercalated disks (ICDs). The main objective of this study was to distinguish between the Ankrd2 isoforms and to determine the contribution of each one to the general profile of Ankrd2 expression in striated muscles. We demonstrated that the known expression and localization pattern of Ankrd2 in striated muscle can be attributed to the isoform of 333 aa which is dominant in both tissues, while the designated cardiac and canonical isoform of 360 aa was less expressed in both tissues. The 360 aa isoform has a distinct nuclear localization in human skeletal muscle, as well as in primary myoblasts and myotubes. In contrast to the isoform of 333 aa, it was not preferentially expressed in slow fibers and not localized to the ICDs of human cardiomyocytes. Regulation of the expression of both isoforms is achieved at the transcriptional level. Our results set the stage for investigation of the specific functions and interactions of the Ankrd2 isoforms in healthy and diseased human striated muscles. PB - Springer, New York T2 - Histochemistry and Cell Biology T1 - Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles EP - 584 IS - 5 SP - 569 VL - 146 DO - 10.1007/s00418-016-1465-0 ER -
@article{ author = "Jasnić, Jovana and Krause, Sabine and Savić, Slobodan and Kojić, Ana and Kovcić, Vlado and Bošković, Srđan and Nestorović, Aleksandra and Rakićević, Ljiljana and Schreiber-Katz, Olivia and Vogel, Johannes G. and Schoser, Benedikt G. and Walter, Maggie C. and Valle, Giorgio and Radojković, Dragica and Faulkner, Georgine and Kojić, Snežana", year = "2016", abstract = "Four human Ankrd2 transcripts, reported in the Ensembl database, code for distinct protein isoforms (360, 333, 327 and 300 aa), and so far, their existence, specific expression and localization patterns have not been studied in detail. Ankrd2 is preferentially expressed in the slow fibers of skeletal muscle. It is found in both the nuclei and the cytoplasm of skeletal muscle cells, and its localization is prone to change during differentiation and upon stress. Ankrd2 has also been detected in the heart, in ventricular cardiomyocytes and in the intercalated disks (ICDs). The main objective of this study was to distinguish between the Ankrd2 isoforms and to determine the contribution of each one to the general profile of Ankrd2 expression in striated muscles. We demonstrated that the known expression and localization pattern of Ankrd2 in striated muscle can be attributed to the isoform of 333 aa which is dominant in both tissues, while the designated cardiac and canonical isoform of 360 aa was less expressed in both tissues. The 360 aa isoform has a distinct nuclear localization in human skeletal muscle, as well as in primary myoblasts and myotubes. In contrast to the isoform of 333 aa, it was not preferentially expressed in slow fibers and not localized to the ICDs of human cardiomyocytes. Regulation of the expression of both isoforms is achieved at the transcriptional level. Our results set the stage for investigation of the specific functions and interactions of the Ankrd2 isoforms in healthy and diseased human striated muscles.", publisher = "Springer, New York", journal = "Histochemistry and Cell Biology", title = "Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles", pages = "584-569", number = "5", volume = "146", doi = "10.1007/s00418-016-1465-0" }
Jasnić, J., Krause, S., Savić, S., Kojić, A., Kovcić, V., Bošković, S., Nestorović, A., Rakićević, L., Schreiber-Katz, O., Vogel, J. G., Schoser, B. G., Walter, M. C., Valle, G., Radojković, D., Faulkner, G.,& Kojić, S.. (2016). Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles. in Histochemistry and Cell Biology Springer, New York., 146(5), 569-584. https://doi.org/10.1007/s00418-016-1465-0
Jasnić J, Krause S, Savić S, Kojić A, Kovcić V, Bošković S, Nestorović A, Rakićević L, Schreiber-Katz O, Vogel JG, Schoser BG, Walter MC, Valle G, Radojković D, Faulkner G, Kojić S. Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles. in Histochemistry and Cell Biology. 2016;146(5):569-584. doi:10.1007/s00418-016-1465-0 .
Jasnić, Jovana, Krause, Sabine, Savić, Slobodan, Kojić, Ana, Kovcić, Vlado, Bošković, Srđan, Nestorović, Aleksandra, Rakićević, Ljiljana, Schreiber-Katz, Olivia, Vogel, Johannes G., Schoser, Benedikt G., Walter, Maggie C., Valle, Giorgio, Radojković, Dragica, Faulkner, Georgine, Kojić, Snežana, "Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles" in Histochemistry and Cell Biology, 146, no. 5 (2016):569-584, https://doi.org/10.1007/s00418-016-1465-0 . .