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Single-step method for beta-galactosidase assays in Escherichia coli using a 96-well microplate reader
dc.creator | Schaefer, Jorrit | |
dc.creator | Jovanović, Goran | |
dc.creator | Kotta-Loizou, Loly | |
dc.creator | Buck, Martin | |
dc.date.accessioned | 2022-11-15T14:42:44Z | |
dc.date.available | 2022-11-15T14:42:44Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0003-2697 | |
dc.identifier.uri | https://imagine.imgge.bg.ac.rs/handle/123456789/935 | |
dc.description.abstract | Historically, the lacZ gene is one of the most universally used reporters of gene expression in molecular biology. Its activity can be quantified using an artificial substrate, o-nitrophenyl-ss-D-galactopyranoside (ONPG). However, the traditional method for measuring LacZ activity (first described by J. H. Miller in 1972) can be challenging for a large number of samples, is prone to variability, and involves hazardous compounds for lysis (e.g., chloroform, toluene). Here we describe a single-step assay using a 96-well microplate reader with a proven alternative cell permeabilization method. This modified protocol reduces handling time by 90%. | en |
dc.publisher | Academic Press Inc Elsevier Science, San Diego | |
dc.relation | Biotechnology and Biological Sciences Research Council (BBSRC) [BB/J00717X/1] | |
dc.relation | U.K. Medical Research Council (MRC) project grants [MR/M017672/1] | |
dc.relation | BBSRC [BB/J00717X/1] Funding Source: UKRI | |
dc.relation | MRC [MR/M017672/1] Funding Source: UKRI | |
dc.relation | Biotechnology and Biological Sciences Research Council [BB/J00717X/1] Funding Source: researchfish | |
dc.relation | Medical Research Council [MR/M017672/1] Funding Source: researchfish | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Analytical Biochemistry | |
dc.subject | Microplate reader | en |
dc.subject | LacZ | en |
dc.subject | B-Galactosidase (Bgal) | en |
dc.subject | beta-Galactosidase | en |
dc.title | Single-step method for beta-galactosidase assays in Escherichia coli using a 96-well microplate reader | en |
dc.type | article | |
dc.rights.license | BY | |
dc.citation.epage | 57 | |
dc.citation.other | 503(): 56-57 | |
dc.citation.rank | M22 | |
dc.citation.spage | 56 | |
dc.citation.volume | 503 | |
dc.identifier.doi | 10.1016/j.ab.2016.03.017 | |
dc.identifier.fulltext | https://imagine.imgge.bg.ac.rs/bitstream/id/838/932.pdf | |
dc.identifier.pmid | 27036618 | |
dc.identifier.scopus | 2-s2.0-84964458891 | |
dc.identifier.wos | 000375895700008 | |
dc.type.version | publishedVersion |