Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis
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2016
Authors
Stanisavljević, S.Lukić, Jovanka
Momcilović, M.
Miljković, M.
Jevtić, B.
Kojić, Milan
Golić, Nataša
Mostarica Stojković, M.
Miljković, D.
Article (Published version)
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Show full item recordAbstract
Gut microbiota and gut-associated lymphoid tissue have been increasingly appreciated as important players in pathogenesis of various autoimmune diseases, including multiple sclerosis. Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis that can be induced with an injection of spinal cord homogenate emulsified in complete Freund's adjuvant in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats. In this study, mesenteric lymph nodes (MLN), Peyer's patches (PP) and gut microbiota were analysed in these two rat strains. There was higher proportion of CD4(+) T cells and regulatory T cells in non-immunised DA rats in comparison to AO rats. Also, DA rat MLN and PP cells were higher producers of pro-inflammatory cytokines interferon-gamma and interleukin-17. Finally, microbial analyses showed that uncultivated species of Turicibacter and Atopostipes genus were exclusively present in AO rats, in faeces and intestinal tissue, respectively. Thus, it is c...lear that in comparison of an EAE-susceptible with an EAE-resistant strain of rats, various discrepancies at the level of gut associated lymphoid tissue, as well as at the level of gut microbiota can be observed. Future studies should determine if the differences have functional significance for EAE pathogenesis.
Keywords:
immune system / gut-associated lymphoid tissues / faeces / DGGE / cytokine / animal modelsSource:
Beneficial Microbes, 2016, 7, 3, 363-373Publisher:
- Wageningen Academic Publishers, Wageningen
Funding / projects:
- Cellular and molecular mechanisms of recovery of rats from experimental autoimmune encephalomyelitis (RS-MESTD-Basic Research (BR or ON)-173035)
- Immunopathogenic and regulatory mechanisms in autoimmune diseases and chronic inflamation (RS-MESTD-Basic Research (BR or ON)-175038)
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.3920/BM2015.0159
ISSN: 1876-2883
PubMed: 26839070
WoS: 000376602400007
Scopus: 2-s2.0-84971646866
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Stanisavljević, S. AU - Lukić, Jovanka AU - Momcilović, M. AU - Miljković, M. AU - Jevtić, B. AU - Kojić, Milan AU - Golić, Nataša AU - Mostarica Stojković, M. AU - Miljković, D. PY - 2016 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/947 AB - Gut microbiota and gut-associated lymphoid tissue have been increasingly appreciated as important players in pathogenesis of various autoimmune diseases, including multiple sclerosis. Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis that can be induced with an injection of spinal cord homogenate emulsified in complete Freund's adjuvant in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats. In this study, mesenteric lymph nodes (MLN), Peyer's patches (PP) and gut microbiota were analysed in these two rat strains. There was higher proportion of CD4(+) T cells and regulatory T cells in non-immunised DA rats in comparison to AO rats. Also, DA rat MLN and PP cells were higher producers of pro-inflammatory cytokines interferon-gamma and interleukin-17. Finally, microbial analyses showed that uncultivated species of Turicibacter and Atopostipes genus were exclusively present in AO rats, in faeces and intestinal tissue, respectively. Thus, it is clear that in comparison of an EAE-susceptible with an EAE-resistant strain of rats, various discrepancies at the level of gut associated lymphoid tissue, as well as at the level of gut microbiota can be observed. Future studies should determine if the differences have functional significance for EAE pathogenesis. PB - Wageningen Academic Publishers, Wageningen T2 - Beneficial Microbes T1 - Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis EP - 373 IS - 3 SP - 363 VL - 7 DO - 10.3920/BM2015.0159 ER -
@article{ author = "Stanisavljević, S. and Lukić, Jovanka and Momcilović, M. and Miljković, M. and Jevtić, B. and Kojić, Milan and Golić, Nataša and Mostarica Stojković, M. and Miljković, D.", year = "2016", abstract = "Gut microbiota and gut-associated lymphoid tissue have been increasingly appreciated as important players in pathogenesis of various autoimmune diseases, including multiple sclerosis. Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis that can be induced with an injection of spinal cord homogenate emulsified in complete Freund's adjuvant in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats. In this study, mesenteric lymph nodes (MLN), Peyer's patches (PP) and gut microbiota were analysed in these two rat strains. There was higher proportion of CD4(+) T cells and regulatory T cells in non-immunised DA rats in comparison to AO rats. Also, DA rat MLN and PP cells were higher producers of pro-inflammatory cytokines interferon-gamma and interleukin-17. Finally, microbial analyses showed that uncultivated species of Turicibacter and Atopostipes genus were exclusively present in AO rats, in faeces and intestinal tissue, respectively. Thus, it is clear that in comparison of an EAE-susceptible with an EAE-resistant strain of rats, various discrepancies at the level of gut associated lymphoid tissue, as well as at the level of gut microbiota can be observed. Future studies should determine if the differences have functional significance for EAE pathogenesis.", publisher = "Wageningen Academic Publishers, Wageningen", journal = "Beneficial Microbes", title = "Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis", pages = "373-363", number = "3", volume = "7", doi = "10.3920/BM2015.0159" }
Stanisavljević, S., Lukić, J., Momcilović, M., Miljković, M., Jevtić, B., Kojić, M., Golić, N., Mostarica Stojković, M.,& Miljković, D.. (2016). Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis. in Beneficial Microbes Wageningen Academic Publishers, Wageningen., 7(3), 363-373. https://doi.org/10.3920/BM2015.0159
Stanisavljević S, Lukić J, Momcilović M, Miljković M, Jevtić B, Kojić M, Golić N, Mostarica Stojković M, Miljković D. Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis. in Beneficial Microbes. 2016;7(3):363-373. doi:10.3920/BM2015.0159 .
Stanisavljević, S., Lukić, Jovanka, Momcilović, M., Miljković, M., Jevtić, B., Kojić, Milan, Golić, Nataša, Mostarica Stojković, M., Miljković, D., "Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis" in Beneficial Microbes, 7, no. 3 (2016):363-373, https://doi.org/10.3920/BM2015.0159 . .