TGFB1 Gene Promoter Polymorphisms in Serbian Asthmatics
Апстракт
Background. Asthma is a chronic respiratory disease caused by a combination of genetic and environmental factors. Transforming growth factor beta 1 (TGFB1) is a multifunctional cytokine that plays an important role in airway remodeling in asthma. Objectives. The aim of this study was to analyze common TGFB1 gene promoter polymorphisms C-509T and G-800A in Serbian asthmatics and to investigate their association with exacerbations. Material and Methods. The study involved 102 asthmatics and 58 healthy individuals from Serbia, age and gender matched. An analysis of the TGFB1 promoter was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results. For polymorphism C-509T a significant difference in the allele frequency was observed between the patients and the controls (p = 0.011), while the genotype distribution was similar in the analyzed groups, with statistical significance near the borderline (p = 0.061). For the polymorphism G-800A no differen...ce was observed between the groups. The frequency of the -509TT genotype was higher in patients with exacerbations compared to patients without exacerbations (36.4% vs. 17.0%), with statistical significance near the borderline (p = 0.080). Conclusions. The results suggest that polymorphism C-509T may be associated with asthma and disease exacerbations, while G-800A is not significant for the etiology and clinical course of the disease. These findings should be confirmed in a larger study group, and since the TGFB1 promoter is highly complex and very responsive to environmental factors, future studies should also take other genetic and non-genetic factors into consideration.
Кључне речи:
transforming growth factor beta 1 / gene polymorphism / asthmaИзвор:
Advances in Clinical and Experimental Medicine, 2016, 25, 2, 273-278Издавач:
- Wroclaw Medical Univ, Wroclaw
Финансирање / пројекти:
- Комплексне болести као модел систем за проучавање модулације фенотипа-структурна и функционална анализа молекуларних биомаркера (RS-MESTD-Basic Research (BR or ON)-173008)
DOI: 10.17219/acem/32211
ISSN: 1899-5276
PubMed: 27627560
WoS: 000376154400009
Scopus: 2-s2.0-84969233483
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Dragičević, Sandra AU - Petrović-Stanojević, Nataša AU - Nikolić, Aleksandra PY - 2016 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/948 AB - Background. Asthma is a chronic respiratory disease caused by a combination of genetic and environmental factors. Transforming growth factor beta 1 (TGFB1) is a multifunctional cytokine that plays an important role in airway remodeling in asthma. Objectives. The aim of this study was to analyze common TGFB1 gene promoter polymorphisms C-509T and G-800A in Serbian asthmatics and to investigate their association with exacerbations. Material and Methods. The study involved 102 asthmatics and 58 healthy individuals from Serbia, age and gender matched. An analysis of the TGFB1 promoter was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results. For polymorphism C-509T a significant difference in the allele frequency was observed between the patients and the controls (p = 0.011), while the genotype distribution was similar in the analyzed groups, with statistical significance near the borderline (p = 0.061). For the polymorphism G-800A no difference was observed between the groups. The frequency of the -509TT genotype was higher in patients with exacerbations compared to patients without exacerbations (36.4% vs. 17.0%), with statistical significance near the borderline (p = 0.080). Conclusions. The results suggest that polymorphism C-509T may be associated with asthma and disease exacerbations, while G-800A is not significant for the etiology and clinical course of the disease. These findings should be confirmed in a larger study group, and since the TGFB1 promoter is highly complex and very responsive to environmental factors, future studies should also take other genetic and non-genetic factors into consideration. PB - Wroclaw Medical Univ, Wroclaw T2 - Advances in Clinical and Experimental Medicine T1 - TGFB1 Gene Promoter Polymorphisms in Serbian Asthmatics EP - 278 IS - 2 SP - 273 VL - 25 DO - 10.17219/acem/32211 ER -
@article{ author = "Dragičević, Sandra and Petrović-Stanojević, Nataša and Nikolić, Aleksandra", year = "2016", abstract = "Background. Asthma is a chronic respiratory disease caused by a combination of genetic and environmental factors. Transforming growth factor beta 1 (TGFB1) is a multifunctional cytokine that plays an important role in airway remodeling in asthma. Objectives. The aim of this study was to analyze common TGFB1 gene promoter polymorphisms C-509T and G-800A in Serbian asthmatics and to investigate their association with exacerbations. Material and Methods. The study involved 102 asthmatics and 58 healthy individuals from Serbia, age and gender matched. An analysis of the TGFB1 promoter was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results. For polymorphism C-509T a significant difference in the allele frequency was observed between the patients and the controls (p = 0.011), while the genotype distribution was similar in the analyzed groups, with statistical significance near the borderline (p = 0.061). For the polymorphism G-800A no difference was observed between the groups. The frequency of the -509TT genotype was higher in patients with exacerbations compared to patients without exacerbations (36.4% vs. 17.0%), with statistical significance near the borderline (p = 0.080). Conclusions. The results suggest that polymorphism C-509T may be associated with asthma and disease exacerbations, while G-800A is not significant for the etiology and clinical course of the disease. These findings should be confirmed in a larger study group, and since the TGFB1 promoter is highly complex and very responsive to environmental factors, future studies should also take other genetic and non-genetic factors into consideration.", publisher = "Wroclaw Medical Univ, Wroclaw", journal = "Advances in Clinical and Experimental Medicine", title = "TGFB1 Gene Promoter Polymorphisms in Serbian Asthmatics", pages = "278-273", number = "2", volume = "25", doi = "10.17219/acem/32211" }
Dragičević, S., Petrović-Stanojević, N.,& Nikolić, A.. (2016). TGFB1 Gene Promoter Polymorphisms in Serbian Asthmatics. in Advances in Clinical and Experimental Medicine Wroclaw Medical Univ, Wroclaw., 25(2), 273-278. https://doi.org/10.17219/acem/32211
Dragičević S, Petrović-Stanojević N, Nikolić A. TGFB1 Gene Promoter Polymorphisms in Serbian Asthmatics. in Advances in Clinical and Experimental Medicine. 2016;25(2):273-278. doi:10.17219/acem/32211 .
Dragičević, Sandra, Petrović-Stanojević, Nataša, Nikolić, Aleksandra, "TGFB1 Gene Promoter Polymorphisms in Serbian Asthmatics" in Advances in Clinical and Experimental Medicine, 25, no. 2 (2016):273-278, https://doi.org/10.17219/acem/32211 . .