ALPHA-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116)
Само за регистроване кориснике
2016
Аутори
Ljujić, MilaMijatović, S.
Bulatović, M. Z.
Mojić, M.
Maksimović-Ivanić, Danijela
Radojković, Dragica
Topić, Aleksandra
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Alpha-1-antitrypsin (AAT), an acute phase protein, is the principal circulatory anti-protease. This multifunctional protein is encoded by the SERPINA1 gene. Although AAT was recognised as a potential tumour marker, its role in cancer biology remains unknown. Given that it has been demonstrated that AAT has an anti-apoptotic property against non-malignant cells, we aimed to investigate whether AAT affects apoptosis in a colon cancer cell line (HCT116). The presence of AAT in the HCT116 cell culture antagonized cytotoxicity of blockers of MEK1/2, PI3K/Akt pathways as well as NF-kappa B. The dominantly recovered cell viability was observed in the co-treatment with MEK1/2 inhibitor U0126. In addition, it was revealed that AAT almost completely abolished U0126-induced apoptosis through maintenance of the autophagy process. Our study revealed for the first time that the observed cyto-protection triggered by AAT was accompanied by sustained autophagy which opposed apoptosis. These results may... contribute to understanding of the role of AAT in cancer development and evaluation of efficacy of cancer therapy.
Кључне речи:
colon cancer cell line (HCT116) / autophagy / apoptosis / alpha-1 antitrypsinИзвор:
Molecular Biology, 2016, 50, 1, 153-156Издавач:
- Maik Nauka/Interperiodica/Springer, New York
Финансирање / пројекти:
- Молекуларни механизми физиолошке и фармаколошке контроле инфламације и канцера (RS-MESTD-Basic Research (BR or ON)-173013)
- Комплексне болести као модел систем за проучавање модулације фенотипа-структурна и функционална анализа молекуларних биомаркера (RS-MESTD-Basic Research (BR or ON)-173008)
DOI: 10.1134/S002689331601012X
ISSN: 0026-8933
WoS: 000378140200018
Scopus: 2-s2.0-84961873039
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Ljujić, Mila AU - Mijatović, S. AU - Bulatović, M. Z. AU - Mojić, M. AU - Maksimović-Ivanić, Danijela AU - Radojković, Dragica AU - Topić, Aleksandra PY - 2016 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/949 AB - Alpha-1-antitrypsin (AAT), an acute phase protein, is the principal circulatory anti-protease. This multifunctional protein is encoded by the SERPINA1 gene. Although AAT was recognised as a potential tumour marker, its role in cancer biology remains unknown. Given that it has been demonstrated that AAT has an anti-apoptotic property against non-malignant cells, we aimed to investigate whether AAT affects apoptosis in a colon cancer cell line (HCT116). The presence of AAT in the HCT116 cell culture antagonized cytotoxicity of blockers of MEK1/2, PI3K/Akt pathways as well as NF-kappa B. The dominantly recovered cell viability was observed in the co-treatment with MEK1/2 inhibitor U0126. In addition, it was revealed that AAT almost completely abolished U0126-induced apoptosis through maintenance of the autophagy process. Our study revealed for the first time that the observed cyto-protection triggered by AAT was accompanied by sustained autophagy which opposed apoptosis. These results may contribute to understanding of the role of AAT in cancer development and evaluation of efficacy of cancer therapy. PB - Maik Nauka/Interperiodica/Springer, New York T2 - Molecular Biology T1 - ALPHA-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116) EP - 156 IS - 1 SP - 153 VL - 50 DO - 10.1134/S002689331601012X ER -
@article{ author = "Ljujić, Mila and Mijatović, S. and Bulatović, M. Z. and Mojić, M. and Maksimović-Ivanić, Danijela and Radojković, Dragica and Topić, Aleksandra", year = "2016", abstract = "Alpha-1-antitrypsin (AAT), an acute phase protein, is the principal circulatory anti-protease. This multifunctional protein is encoded by the SERPINA1 gene. Although AAT was recognised as a potential tumour marker, its role in cancer biology remains unknown. Given that it has been demonstrated that AAT has an anti-apoptotic property against non-malignant cells, we aimed to investigate whether AAT affects apoptosis in a colon cancer cell line (HCT116). The presence of AAT in the HCT116 cell culture antagonized cytotoxicity of blockers of MEK1/2, PI3K/Akt pathways as well as NF-kappa B. The dominantly recovered cell viability was observed in the co-treatment with MEK1/2 inhibitor U0126. In addition, it was revealed that AAT almost completely abolished U0126-induced apoptosis through maintenance of the autophagy process. Our study revealed for the first time that the observed cyto-protection triggered by AAT was accompanied by sustained autophagy which opposed apoptosis. These results may contribute to understanding of the role of AAT in cancer development and evaluation of efficacy of cancer therapy.", publisher = "Maik Nauka/Interperiodica/Springer, New York", journal = "Molecular Biology", title = "ALPHA-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116)", pages = "156-153", number = "1", volume = "50", doi = "10.1134/S002689331601012X" }
Ljujić, M., Mijatović, S., Bulatović, M. Z., Mojić, M., Maksimović-Ivanić, D., Radojković, D.,& Topić, A.. (2016). ALPHA-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116). in Molecular Biology Maik Nauka/Interperiodica/Springer, New York., 50(1), 153-156. https://doi.org/10.1134/S002689331601012X
Ljujić M, Mijatović S, Bulatović MZ, Mojić M, Maksimović-Ivanić D, Radojković D, Topić A. ALPHA-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116). in Molecular Biology. 2016;50(1):153-156. doi:10.1134/S002689331601012X .
Ljujić, Mila, Mijatović, S., Bulatović, M. Z., Mojić, M., Maksimović-Ivanić, Danijela, Radojković, Dragica, Topić, Aleksandra, "ALPHA-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116)" in Molecular Biology, 50, no. 1 (2016):153-156, https://doi.org/10.1134/S002689331601012X . .