Identification, nucleotide sequence, and characterization of PspF, the transcriptional activator of the Escherichia coli stress-induced psp operon
Апстракт
The phage shock protein (psp) operon (pspABCE) of Escherichia coli is strongly induced in response to a variety of stressful conditions or agents such as filamentous phage infection, ethanol treatment, osmotic shock, heat shock, and prolonged incubation in stationary phase. Transcription of the psp operon is driven from a sigma(54) promoter and stimulated by integration host factor. We report here the identification of a transcriptional activator gene, designated pspF, which controls expression of the psp operon in E. coli. The pspF gene was identified by random miniTn10-tet transposon mutagenesis. Insertion of the transposon into the pspF gene abolished sigma(54)-dependent induction of the psp operon. The pspF gene is closely linked to the psp operon and is divergently transcribed from one major and two minor sigma(70) promoters. pspF encodes a 37-kDa protein which belongs to the enhancer-binding protein family of sigma(54) transcriptional activators. PspF contains a catalytic domain,... which in other sigma(54) activators mould be the central domain, and a C-terminal DNA-binding domain but entirely lacks an N-terminal regulatory domain and is constitutively active. The insertion mutant pspF::mTn10-tet (pspF(877)) encodes a truncated protein (PspF Delta HTH) that lacks the DNA-binding helix-turn-helix (HTH) motif. Although the central catalytic domain is intact, PspF Delta HTH at physiological concentration cannot activate psp expression. In the absence of inducing stimuli, multicopy-plasmid-borne PspF or PspF Delta HTH overcomes repression of the psp operon mediated by the negative regulator PspA.
Извор:
Journal of Bacteriology, 1996, 178, 7, 1936-1945Издавач:
- Amer Soc Microbiology, Washington
Финансирање / пројекти:
- NIAID NIH HHS [AIO7223] Funding Source: Medline
DOI: 10.1128/jb.178.7.1936-1945.1996
ISSN: 0021-9193
PubMed: 8606168
WoS: A1996UD48400022
Scopus: 2-s2.0-0029874910
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Jovanović, Goran AU - Weiner, L AU - Model, P PY - 1996 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/95 AB - The phage shock protein (psp) operon (pspABCE) of Escherichia coli is strongly induced in response to a variety of stressful conditions or agents such as filamentous phage infection, ethanol treatment, osmotic shock, heat shock, and prolonged incubation in stationary phase. Transcription of the psp operon is driven from a sigma(54) promoter and stimulated by integration host factor. We report here the identification of a transcriptional activator gene, designated pspF, which controls expression of the psp operon in E. coli. The pspF gene was identified by random miniTn10-tet transposon mutagenesis. Insertion of the transposon into the pspF gene abolished sigma(54)-dependent induction of the psp operon. The pspF gene is closely linked to the psp operon and is divergently transcribed from one major and two minor sigma(70) promoters. pspF encodes a 37-kDa protein which belongs to the enhancer-binding protein family of sigma(54) transcriptional activators. PspF contains a catalytic domain, which in other sigma(54) activators mould be the central domain, and a C-terminal DNA-binding domain but entirely lacks an N-terminal regulatory domain and is constitutively active. The insertion mutant pspF::mTn10-tet (pspF(877)) encodes a truncated protein (PspF Delta HTH) that lacks the DNA-binding helix-turn-helix (HTH) motif. Although the central catalytic domain is intact, PspF Delta HTH at physiological concentration cannot activate psp expression. In the absence of inducing stimuli, multicopy-plasmid-borne PspF or PspF Delta HTH overcomes repression of the psp operon mediated by the negative regulator PspA. PB - Amer Soc Microbiology, Washington T2 - Journal of Bacteriology T1 - Identification, nucleotide sequence, and characterization of PspF, the transcriptional activator of the Escherichia coli stress-induced psp operon EP - 1945 IS - 7 SP - 1936 VL - 178 DO - 10.1128/jb.178.7.1936-1945.1996 ER -
@article{ author = "Jovanović, Goran and Weiner, L and Model, P", year = "1996", abstract = "The phage shock protein (psp) operon (pspABCE) of Escherichia coli is strongly induced in response to a variety of stressful conditions or agents such as filamentous phage infection, ethanol treatment, osmotic shock, heat shock, and prolonged incubation in stationary phase. Transcription of the psp operon is driven from a sigma(54) promoter and stimulated by integration host factor. We report here the identification of a transcriptional activator gene, designated pspF, which controls expression of the psp operon in E. coli. The pspF gene was identified by random miniTn10-tet transposon mutagenesis. Insertion of the transposon into the pspF gene abolished sigma(54)-dependent induction of the psp operon. The pspF gene is closely linked to the psp operon and is divergently transcribed from one major and two minor sigma(70) promoters. pspF encodes a 37-kDa protein which belongs to the enhancer-binding protein family of sigma(54) transcriptional activators. PspF contains a catalytic domain, which in other sigma(54) activators mould be the central domain, and a C-terminal DNA-binding domain but entirely lacks an N-terminal regulatory domain and is constitutively active. The insertion mutant pspF::mTn10-tet (pspF(877)) encodes a truncated protein (PspF Delta HTH) that lacks the DNA-binding helix-turn-helix (HTH) motif. Although the central catalytic domain is intact, PspF Delta HTH at physiological concentration cannot activate psp expression. In the absence of inducing stimuli, multicopy-plasmid-borne PspF or PspF Delta HTH overcomes repression of the psp operon mediated by the negative regulator PspA.", publisher = "Amer Soc Microbiology, Washington", journal = "Journal of Bacteriology", title = "Identification, nucleotide sequence, and characterization of PspF, the transcriptional activator of the Escherichia coli stress-induced psp operon", pages = "1945-1936", number = "7", volume = "178", doi = "10.1128/jb.178.7.1936-1945.1996" }
Jovanović, G., Weiner, L.,& Model, P.. (1996). Identification, nucleotide sequence, and characterization of PspF, the transcriptional activator of the Escherichia coli stress-induced psp operon. in Journal of Bacteriology Amer Soc Microbiology, Washington., 178(7), 1936-1945. https://doi.org/10.1128/jb.178.7.1936-1945.1996
Jovanović G, Weiner L, Model P. Identification, nucleotide sequence, and characterization of PspF, the transcriptional activator of the Escherichia coli stress-induced psp operon. in Journal of Bacteriology. 1996;178(7):1936-1945. doi:10.1128/jb.178.7.1936-1945.1996 .
Jovanović, Goran, Weiner, L, Model, P, "Identification, nucleotide sequence, and characterization of PspF, the transcriptional activator of the Escherichia coli stress-induced psp operon" in Journal of Bacteriology, 178, no. 7 (1996):1936-1945, https://doi.org/10.1128/jb.178.7.1936-1945.1996 . .