Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species
2016
Authors
Pekmezović, MarinaAleksić, Ivana
Barac, Aleksandra
Arsić-Arsenijević, Valentina
Vasiljević, Branka
Nikodinović-Runić, Jasmina
Šenerović, Lidija
Article (Published version)
Metadata
Show full item recordAbstract
Mixed microbial infections caused by Pseudomonas aeruginosa and pathogenic fungi are commonly found in patients with chronic infections and constitute a significant health care burden. The aim of this study was to address the potential polymicrobial antibiofilm activity of pompia and grapefruit essential oils (EOs). The mechanism of antimicrobial activity of EOs was analysed. EOs of pompia and grapefruit inhibited fungal growth with MIC concentrations between 50 and 250 mg L-1, whereas no effect on P. aeruginosa growth was observed. Both citrus EOs inhibited formation of bacterial and fungal monomicrobial biofilms in concentrations of 50 mg L-1 and were efficient in potentiating the activity of clinically used antimicrobials in vitro. The concentration of 10 mg L-1 EOs inhibited mixed biofilm formation composed of P. aeruginosa and Aspergillus fumigatus or Scedosporium apiospermum. Citrus EOs affected quorum sensing in P. aeruginosa and caused fast permeabilisation of Candida albicans ...membrane. Pompia and grapefruit EOs potently inhibited biofilm formation and could be used for the control of common polymicrobial infections.
Keywords:
quorum sensing / Pseudomonas aeruginosa / polymicrobial biofilms / essential oils / antifungalSource:
Pathogens and Disease, 2016, 74, 8Publisher:
- Oxford Univ Press, Oxford
Funding / projects:
- European Society of Clinical Microbiology and Infectious Diseases (ESCMID)
- Microbial diversity study and characterization of beneficial environmental microorganisms (RS-MESTD-Basic Research (BR or ON)-173048)
- Detection of early laboratory fungal biomarkers and it's importance for outcome of invasive fungal infections in Serbia (RS-MESTD-Basic Research (BR or ON)-175034)
DOI: 10.1093/femspd/ftw102
ISSN: 2049-632X
PubMed: 27702795
WoS: 000394690300013
Scopus: 2-s2.0-85019755424
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Pekmezović, Marina AU - Aleksić, Ivana AU - Barac, Aleksandra AU - Arsić-Arsenijević, Valentina AU - Vasiljević, Branka AU - Nikodinović-Runić, Jasmina AU - Šenerović, Lidija PY - 2016 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/953 AB - Mixed microbial infections caused by Pseudomonas aeruginosa and pathogenic fungi are commonly found in patients with chronic infections and constitute a significant health care burden. The aim of this study was to address the potential polymicrobial antibiofilm activity of pompia and grapefruit essential oils (EOs). The mechanism of antimicrobial activity of EOs was analysed. EOs of pompia and grapefruit inhibited fungal growth with MIC concentrations between 50 and 250 mg L-1, whereas no effect on P. aeruginosa growth was observed. Both citrus EOs inhibited formation of bacterial and fungal monomicrobial biofilms in concentrations of 50 mg L-1 and were efficient in potentiating the activity of clinically used antimicrobials in vitro. The concentration of 10 mg L-1 EOs inhibited mixed biofilm formation composed of P. aeruginosa and Aspergillus fumigatus or Scedosporium apiospermum. Citrus EOs affected quorum sensing in P. aeruginosa and caused fast permeabilisation of Candida albicans membrane. Pompia and grapefruit EOs potently inhibited biofilm formation and could be used for the control of common polymicrobial infections. PB - Oxford Univ Press, Oxford T2 - Pathogens and Disease T1 - Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species IS - 8 VL - 74 DO - 10.1093/femspd/ftw102 ER -
@article{ author = "Pekmezović, Marina and Aleksić, Ivana and Barac, Aleksandra and Arsić-Arsenijević, Valentina and Vasiljević, Branka and Nikodinović-Runić, Jasmina and Šenerović, Lidija", year = "2016", abstract = "Mixed microbial infections caused by Pseudomonas aeruginosa and pathogenic fungi are commonly found in patients with chronic infections and constitute a significant health care burden. The aim of this study was to address the potential polymicrobial antibiofilm activity of pompia and grapefruit essential oils (EOs). The mechanism of antimicrobial activity of EOs was analysed. EOs of pompia and grapefruit inhibited fungal growth with MIC concentrations between 50 and 250 mg L-1, whereas no effect on P. aeruginosa growth was observed. Both citrus EOs inhibited formation of bacterial and fungal monomicrobial biofilms in concentrations of 50 mg L-1 and were efficient in potentiating the activity of clinically used antimicrobials in vitro. The concentration of 10 mg L-1 EOs inhibited mixed biofilm formation composed of P. aeruginosa and Aspergillus fumigatus or Scedosporium apiospermum. Citrus EOs affected quorum sensing in P. aeruginosa and caused fast permeabilisation of Candida albicans membrane. Pompia and grapefruit EOs potently inhibited biofilm formation and could be used for the control of common polymicrobial infections.", publisher = "Oxford Univ Press, Oxford", journal = "Pathogens and Disease", title = "Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species", number = "8", volume = "74", doi = "10.1093/femspd/ftw102" }
Pekmezović, M., Aleksić, I., Barac, A., Arsić-Arsenijević, V., Vasiljević, B., Nikodinović-Runić, J.,& Šenerović, L.. (2016). Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species. in Pathogens and Disease Oxford Univ Press, Oxford., 74(8). https://doi.org/10.1093/femspd/ftw102
Pekmezović M, Aleksić I, Barac A, Arsić-Arsenijević V, Vasiljević B, Nikodinović-Runić J, Šenerović L. Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species. in Pathogens and Disease. 2016;74(8). doi:10.1093/femspd/ftw102 .
Pekmezović, Marina, Aleksić, Ivana, Barac, Aleksandra, Arsić-Arsenijević, Valentina, Vasiljević, Branka, Nikodinović-Runić, Jasmina, Šenerović, Lidija, "Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species" in Pathogens and Disease, 74, no. 8 (2016), https://doi.org/10.1093/femspd/ftw102 . .