EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells
2016
Аутори
Živković, MilicaMiljković, Marge S.
Ruas-Madiedo, Patricia
Markelić, Milica B.
Veljović, Katarina
Tolinački, Maja
Soković Bajić, Svetlana
Korac, Aleksandra
Golić, Nataša
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to determine the role of an exopolysaccharide produced by natural dairy isolate Lactobacillus paracasel subsp. paracasel BGSJ2-8, in the adhesion to intestinal epithelial cells and a decrease in Escherichia coil's association with Caco-2 cells. Annotation of the BGSJ2-8 genome showed the presence of a gene cluster, epsSJ, which encodes the biosynthesis of the strain-specific exopolysaccharide EPS-SJ, detected as two fractions (P1 and P2) by size exclusion chromatography (SEC) coupled with multi angle laser light scattering (MALLS) detection. SEC MALLS analysis revealed that an EPS-SJ(-) mutant (EPS7, obtained by insertion mutagenesis of the glps_2198 gene encoding primary glycosyltransferase) does not produce the P2 fraction of EPS-SJ. Transmission electron microscopy showed that EPS7 mutant has a thinner cell wall compared to the EPS-SJ(+) strain BGSJ2-83 (a plasmid free derivative of BGSJ2-8). Interestingly, strain BGSJ2-83 showed higher adhesion to Caco-2 e...pithelial intestinal cell line than the EPS7 mutant. Accordingly, BGSJ2-83 effectively reduced E. coil ATCC25922's association with Caco-2 cells, while EPS7 did not show statistically significant differences. In addition, the effect of EPS-SJ on the proliferation of lymphocytes in gastrointestinal associated lymphoid tissue (GALT) was tested and the results showed that the reduction of GALT lymphocyte proliferation was higher by BGSJ2-83 than by the mutant. To the best of our knowledge this is the first report indicating that the presence of EPS (EPS-SJ) on the surface of lactobacilli can improve communication between bacteria and intestinal epithelium, implying its possible role in gut colonization.
Кључне речи:
Lactobacillus paracasei / GALT lymphocytes / EPS-SJ / E. coil association / adhesionИзвор:
Frontiers in Microbiology, 2016, 7Издавач:
- Frontiers Media Sa, Lausanne
Финансирање / пројекти:
- Изучавање гена и молекуларних механизама у основи пробиотичке активности бактерија млечне киселине изолованих са подручја западног Балкана (RS-MESTD-Basic Research (BR or ON)-173019)
DOI: 10.3389/fmicb.2016.00286
ISSN: 1664-302X
WoS: 000371543300001
Scopus: 2-s2.0-84964375038
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Živković, Milica AU - Miljković, Marge S. AU - Ruas-Madiedo, Patricia AU - Markelić, Milica B. AU - Veljović, Katarina AU - Tolinački, Maja AU - Soković Bajić, Svetlana AU - Korac, Aleksandra AU - Golić, Nataša PY - 2016 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/989 AB - The aim of this study was to determine the role of an exopolysaccharide produced by natural dairy isolate Lactobacillus paracasel subsp. paracasel BGSJ2-8, in the adhesion to intestinal epithelial cells and a decrease in Escherichia coil's association with Caco-2 cells. Annotation of the BGSJ2-8 genome showed the presence of a gene cluster, epsSJ, which encodes the biosynthesis of the strain-specific exopolysaccharide EPS-SJ, detected as two fractions (P1 and P2) by size exclusion chromatography (SEC) coupled with multi angle laser light scattering (MALLS) detection. SEC MALLS analysis revealed that an EPS-SJ(-) mutant (EPS7, obtained by insertion mutagenesis of the glps_2198 gene encoding primary glycosyltransferase) does not produce the P2 fraction of EPS-SJ. Transmission electron microscopy showed that EPS7 mutant has a thinner cell wall compared to the EPS-SJ(+) strain BGSJ2-83 (a plasmid free derivative of BGSJ2-8). Interestingly, strain BGSJ2-83 showed higher adhesion to Caco-2 epithelial intestinal cell line than the EPS7 mutant. Accordingly, BGSJ2-83 effectively reduced E. coil ATCC25922's association with Caco-2 cells, while EPS7 did not show statistically significant differences. In addition, the effect of EPS-SJ on the proliferation of lymphocytes in gastrointestinal associated lymphoid tissue (GALT) was tested and the results showed that the reduction of GALT lymphocyte proliferation was higher by BGSJ2-83 than by the mutant. To the best of our knowledge this is the first report indicating that the presence of EPS (EPS-SJ) on the surface of lactobacilli can improve communication between bacteria and intestinal epithelium, implying its possible role in gut colonization. PB - Frontiers Media Sa, Lausanne T2 - Frontiers in Microbiology T1 - EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells VL - 7 DO - 10.3389/fmicb.2016.00286 ER -
@article{ author = "Živković, Milica and Miljković, Marge S. and Ruas-Madiedo, Patricia and Markelić, Milica B. and Veljović, Katarina and Tolinački, Maja and Soković Bajić, Svetlana and Korac, Aleksandra and Golić, Nataša", year = "2016", abstract = "The aim of this study was to determine the role of an exopolysaccharide produced by natural dairy isolate Lactobacillus paracasel subsp. paracasel BGSJ2-8, in the adhesion to intestinal epithelial cells and a decrease in Escherichia coil's association with Caco-2 cells. Annotation of the BGSJ2-8 genome showed the presence of a gene cluster, epsSJ, which encodes the biosynthesis of the strain-specific exopolysaccharide EPS-SJ, detected as two fractions (P1 and P2) by size exclusion chromatography (SEC) coupled with multi angle laser light scattering (MALLS) detection. SEC MALLS analysis revealed that an EPS-SJ(-) mutant (EPS7, obtained by insertion mutagenesis of the glps_2198 gene encoding primary glycosyltransferase) does not produce the P2 fraction of EPS-SJ. Transmission electron microscopy showed that EPS7 mutant has a thinner cell wall compared to the EPS-SJ(+) strain BGSJ2-83 (a plasmid free derivative of BGSJ2-8). Interestingly, strain BGSJ2-83 showed higher adhesion to Caco-2 epithelial intestinal cell line than the EPS7 mutant. Accordingly, BGSJ2-83 effectively reduced E. coil ATCC25922's association with Caco-2 cells, while EPS7 did not show statistically significant differences. In addition, the effect of EPS-SJ on the proliferation of lymphocytes in gastrointestinal associated lymphoid tissue (GALT) was tested and the results showed that the reduction of GALT lymphocyte proliferation was higher by BGSJ2-83 than by the mutant. To the best of our knowledge this is the first report indicating that the presence of EPS (EPS-SJ) on the surface of lactobacilli can improve communication between bacteria and intestinal epithelium, implying its possible role in gut colonization.", publisher = "Frontiers Media Sa, Lausanne", journal = "Frontiers in Microbiology", title = "EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells", volume = "7", doi = "10.3389/fmicb.2016.00286" }
Živković, M., Miljković, M. S., Ruas-Madiedo, P., Markelić, M. B., Veljović, K., Tolinački, M., Soković Bajić, S., Korac, A.,& Golić, N.. (2016). EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells. in Frontiers in Microbiology Frontiers Media Sa, Lausanne., 7. https://doi.org/10.3389/fmicb.2016.00286
Živković M, Miljković MS, Ruas-Madiedo P, Markelić MB, Veljović K, Tolinački M, Soković Bajić S, Korac A, Golić N. EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells. in Frontiers in Microbiology. 2016;7. doi:10.3389/fmicb.2016.00286 .
Živković, Milica, Miljković, Marge S., Ruas-Madiedo, Patricia, Markelić, Milica B., Veljović, Katarina, Tolinački, Maja, Soković Bajić, Svetlana, Korac, Aleksandra, Golić, Nataša, "EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells" in Frontiers in Microbiology, 7 (2016), https://doi.org/10.3389/fmicb.2016.00286 . .