Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display
Апстракт
Microbial surface and secreted proteins (the secretome) contain a large number of proteins that interact with other microbes, host and/or environment. These proteins are exported by the coordinated activities of the protein secretion machinery present in the cell. A group of bacteriophage, called filamentous phage, have the ability to hijack bacterial protein secretion machinery in order to amplify and assemble via a secretion-like process. This ability has been harnessed in the use of filamentous phage of Escherichia coli in biotechnology applications, including screening large libraries of variants for binding to "bait" of interest, from tissues in vivo to pure proteins or even inorganic substrates. In this review we discuss the roles of secretome proteins in pathogenic and non-pathogenic bacteria and corresponding secretion pathways. We describe the basics of phage display technology and its variants applied to discovery of bacterial proteins that are implicated in colonization of h...ost tissues and pathogenesis, as well as vaccine candidates through filamentous phage display library screening. Secretome selection aided by next-generation sequence analysis was successfully applied for selective display of the secretome at a microbial community scale, the latter revealing the richness of secretome functions of interest and surprising versatility in filamentous phage display of secretome proteins from large number of Gram-negative as well as Gram-positive bacteria and archaea.
Кључне речи:
secretome / phage display / next generation sequencing / metagenomics / bacteriophage / adhesinsИзвор:
Frontiers in Microbiology, 2016, 7Издавач:
- Frontiers Media Sa, Lausanne
Финансирање / пројекти:
- New Zealand Ministry for Business, Innovation and Employment grant [C10X0803]
- Royal Society of New Zealand through a Marsden Fast Start grant [AGR1003]
- Commonwealth Ph.D. Fellowship
- Institute of Fundamental Sciences (Massey University) Ph.D. scholarship
- Massey University
DOI: 10.3389/fmicb.2016.00429
ISSN: 1664-302X
WoS: 000373591900001
Scopus: 2-s2.0-84966269370
Институција/група
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Gagić, Dragana AU - Ćirić, Milica AU - Wen, Wesley X. AU - Ng, Filomena AU - Rakonjac, Jasna PY - 2016 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/993 AB - Microbial surface and secreted proteins (the secretome) contain a large number of proteins that interact with other microbes, host and/or environment. These proteins are exported by the coordinated activities of the protein secretion machinery present in the cell. A group of bacteriophage, called filamentous phage, have the ability to hijack bacterial protein secretion machinery in order to amplify and assemble via a secretion-like process. This ability has been harnessed in the use of filamentous phage of Escherichia coli in biotechnology applications, including screening large libraries of variants for binding to "bait" of interest, from tissues in vivo to pure proteins or even inorganic substrates. In this review we discuss the roles of secretome proteins in pathogenic and non-pathogenic bacteria and corresponding secretion pathways. We describe the basics of phage display technology and its variants applied to discovery of bacterial proteins that are implicated in colonization of host tissues and pathogenesis, as well as vaccine candidates through filamentous phage display library screening. Secretome selection aided by next-generation sequence analysis was successfully applied for selective display of the secretome at a microbial community scale, the latter revealing the richness of secretome functions of interest and surprising versatility in filamentous phage display of secretome proteins from large number of Gram-negative as well as Gram-positive bacteria and archaea. PB - Frontiers Media Sa, Lausanne T2 - Frontiers in Microbiology T1 - Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display VL - 7 DO - 10.3389/fmicb.2016.00429 ER -
@article{ author = "Gagić, Dragana and Ćirić, Milica and Wen, Wesley X. and Ng, Filomena and Rakonjac, Jasna", year = "2016", abstract = "Microbial surface and secreted proteins (the secretome) contain a large number of proteins that interact with other microbes, host and/or environment. These proteins are exported by the coordinated activities of the protein secretion machinery present in the cell. A group of bacteriophage, called filamentous phage, have the ability to hijack bacterial protein secretion machinery in order to amplify and assemble via a secretion-like process. This ability has been harnessed in the use of filamentous phage of Escherichia coli in biotechnology applications, including screening large libraries of variants for binding to "bait" of interest, from tissues in vivo to pure proteins or even inorganic substrates. In this review we discuss the roles of secretome proteins in pathogenic and non-pathogenic bacteria and corresponding secretion pathways. We describe the basics of phage display technology and its variants applied to discovery of bacterial proteins that are implicated in colonization of host tissues and pathogenesis, as well as vaccine candidates through filamentous phage display library screening. Secretome selection aided by next-generation sequence analysis was successfully applied for selective display of the secretome at a microbial community scale, the latter revealing the richness of secretome functions of interest and surprising versatility in filamentous phage display of secretome proteins from large number of Gram-negative as well as Gram-positive bacteria and archaea.", publisher = "Frontiers Media Sa, Lausanne", journal = "Frontiers in Microbiology", title = "Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display", volume = "7", doi = "10.3389/fmicb.2016.00429" }
Gagić, D., Ćirić, M., Wen, W. X., Ng, F.,& Rakonjac, J.. (2016). Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display. in Frontiers in Microbiology Frontiers Media Sa, Lausanne., 7. https://doi.org/10.3389/fmicb.2016.00429
Gagić D, Ćirić M, Wen WX, Ng F, Rakonjac J. Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display. in Frontiers in Microbiology. 2016;7. doi:10.3389/fmicb.2016.00429 .
Gagić, Dragana, Ćirić, Milica, Wen, Wesley X., Ng, Filomena, Rakonjac, Jasna, "Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display" in Frontiers in Microbiology, 7 (2016), https://doi.org/10.3389/fmicb.2016.00429 . .