SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line
Authors
Stanisavljević Ninković, DanijelaPetrović, Isidora
Vuković, Vladanka
Schwirtlich, Marija
Gredić, Marija
Stevanović, Milena
Popović, Jelena
Article (Published version)
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SOX14 is a member of the SOX family of transcription factors mainly involved in the regulation of neural development. Recently, it became evident that SOX14 is one of four hyper-methylated genes in cervical carcinoma, considered as a tumor suppressor candidate in this type of malignancy. In this paper we elucidated the role of SOX14 in the regulation of malignant properties of cervical carcinoma cells in vitro. Functional analysis performed in HeLa cells revealed that SOX14 overexpression decreased viability and promoted apoptosis through altering the expression of apoptosis related genes. Our results demonstrated that overexpression of SOX14 initiated accumulation of p53, demonstrating potential cross-talk between SOX14 and the p53 signaling pathway. Further analysis unambiguously showed that SOX14 triggered posttranslational modification of p53 protein, as detected by the significantly increased level of phospho-p53 (Ser-15) in SOX14-overexpressing HeLa cells. Moreover, the obtained ...results revealed that SOX14 activated p53 protein, which was confirmed by elevated p21 Waf1/Cip1, a well known target gene of p53. This study advances our understanding about the role of SOX14 and might explain the molecular mechanism by which this transcription factor could exert tumor suppressor properties in cervical carcinoma.
Source:
PLoS One, 2017, 12, 9Publisher:
- Public Library Science, San Francisco
Funding / projects:
- Studying signal transduction pathways and epigenetic mechanisms that control human SOX genes expression: further insight into their roles in cell fate determination and differentiation (RS-MESTD-Basic Research (BR or ON)-173051)
- Serbian Academy of Sciences and Arts (Project: "Studying the molecular mechanisms involved in maintaining pluripotency and differentiation of stem cells" [F 24]
- Northwestern University RHLCCC Flow Cytometry Facility
- Cancer Center Support Grant [NCI CA060553]
- NATIONAL CANCER INSTITUTE [P30CA060553] Funding Source: NIH RePORTER
DOI: 10.1371/journal.pone.0184686
ISSN: 1932-6203
PubMed: 28926586
WoS: 000411166600034
Scopus: 2-s2.0-85029782145
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Institution/Community
Institut za molekularnu genetiku i genetičko inženjerstvoTY - JOUR AU - Stanisavljević Ninković, Danijela AU - Petrović, Isidora AU - Vuković, Vladanka AU - Schwirtlich, Marija AU - Gredić, Marija AU - Stevanović, Milena AU - Popović, Jelena PY - 2017 UR - https://imagine.imgge.bg.ac.rs/handle/123456789/996 AB - SOX14 is a member of the SOX family of transcription factors mainly involved in the regulation of neural development. Recently, it became evident that SOX14 is one of four hyper-methylated genes in cervical carcinoma, considered as a tumor suppressor candidate in this type of malignancy. In this paper we elucidated the role of SOX14 in the regulation of malignant properties of cervical carcinoma cells in vitro. Functional analysis performed in HeLa cells revealed that SOX14 overexpression decreased viability and promoted apoptosis through altering the expression of apoptosis related genes. Our results demonstrated that overexpression of SOX14 initiated accumulation of p53, demonstrating potential cross-talk between SOX14 and the p53 signaling pathway. Further analysis unambiguously showed that SOX14 triggered posttranslational modification of p53 protein, as detected by the significantly increased level of phospho-p53 (Ser-15) in SOX14-overexpressing HeLa cells. Moreover, the obtained results revealed that SOX14 activated p53 protein, which was confirmed by elevated p21 Waf1/Cip1, a well known target gene of p53. This study advances our understanding about the role of SOX14 and might explain the molecular mechanism by which this transcription factor could exert tumor suppressor properties in cervical carcinoma. PB - Public Library Science, San Francisco T2 - PLoS One T1 - SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line IS - 9 VL - 12 DO - 10.1371/journal.pone.0184686 ER -
@article{ author = "Stanisavljević Ninković, Danijela and Petrović, Isidora and Vuković, Vladanka and Schwirtlich, Marija and Gredić, Marija and Stevanović, Milena and Popović, Jelena", year = "2017", abstract = "SOX14 is a member of the SOX family of transcription factors mainly involved in the regulation of neural development. Recently, it became evident that SOX14 is one of four hyper-methylated genes in cervical carcinoma, considered as a tumor suppressor candidate in this type of malignancy. In this paper we elucidated the role of SOX14 in the regulation of malignant properties of cervical carcinoma cells in vitro. Functional analysis performed in HeLa cells revealed that SOX14 overexpression decreased viability and promoted apoptosis through altering the expression of apoptosis related genes. Our results demonstrated that overexpression of SOX14 initiated accumulation of p53, demonstrating potential cross-talk between SOX14 and the p53 signaling pathway. Further analysis unambiguously showed that SOX14 triggered posttranslational modification of p53 protein, as detected by the significantly increased level of phospho-p53 (Ser-15) in SOX14-overexpressing HeLa cells. Moreover, the obtained results revealed that SOX14 activated p53 protein, which was confirmed by elevated p21 Waf1/Cip1, a well known target gene of p53. This study advances our understanding about the role of SOX14 and might explain the molecular mechanism by which this transcription factor could exert tumor suppressor properties in cervical carcinoma.", publisher = "Public Library Science, San Francisco", journal = "PLoS One", title = "SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line", number = "9", volume = "12", doi = "10.1371/journal.pone.0184686" }
Stanisavljević Ninković, D., Petrović, I., Vuković, V., Schwirtlich, M., Gredić, M., Stevanović, M.,& Popović, J.. (2017). SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line. in PLoS One Public Library Science, San Francisco., 12(9). https://doi.org/10.1371/journal.pone.0184686
Stanisavljević Ninković D, Petrović I, Vuković V, Schwirtlich M, Gredić M, Stevanović M, Popović J. SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line. in PLoS One. 2017;12(9). doi:10.1371/journal.pone.0184686 .
Stanisavljević Ninković, Danijela, Petrović, Isidora, Vuković, Vladanka, Schwirtlich, Marija, Gredić, Marija, Stevanović, Milena, Popović, Jelena, "SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line" in PLoS One, 12, no. 9 (2017), https://doi.org/10.1371/journal.pone.0184686 . .