TY  - JOUR
AU  - Kotur, Nikola
AU  - Skakić, Anita
AU  - Klaassen, Kristel
AU  - Gašić, Vladimir
AU  - Zukić, Branka
AU  - Skodrić-Trifunović, Vesna
AU  - Stjepanović, Mihailo
AU  - Zivković, Zorica
AU  - Ostojić, Olivera
AU  - Stevanović, Goran
AU  - Lavadinović, Lidija
AU  - Pavlović, Sonja
AU  - Stanković, Biljana
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1439
AB  - Background: COVID-19 pandemic has proved to be an unrelenting health threat for more than a year now. The emerging amount of data indicates that vitamin D, zinc and selenium could be important for clinical presentation of COVID-19. Here, we investigated association of genetic variants related to the altered level and bioavailability of vitamin D, zinc and selenium with clinical severity of COVID-19. Methods: We analyzed variants in genes significant for the status of vitamin D (DHCR7/NADSYN1 rs12785878, GC rs2282679, CYP2R1 rs10741657, and VDR rs2228570), zinc (PPCDC rs2120019) and selenium (DMGDH rs17823744) in 120 Serbian adult and pediatric COVID-19 patients using allelic discrimination. Furthermore, we carried out comparative population genetic analysis among European and other worldwide populations to investigate variation in allelic frequencies of selected variants. Results: Study showed that DHCR7/NADSYN rs12785878 and CYP2R1 rs10741657 variants were associated with severe COVID-19 in adults (p = 0.03, p = 0.017, respectively); carriers of DHCR7/NADSYN TG+GG and CYP2R1 GG genotypes had 0.21 and 5.9 the odds for developing severe disease, OR 0.21 (0.05-0.9) and OR 5.9 (1.4-25.2), respectively. There were no associations between selected genetic variants and disease severity in pediatric patients. Comparative population genetic analysis revealed that Serbian population had the lowest frequency of CYP2R1 rs10741657 G allele compared to other non-Finish Europeans (0.58 compared to 0.69 and 0.66 in Spanish and Italian population, respectively), suggesting that other populations should also investigate the relationship of CYP2R1 variant and the COVID-19 disease course. Conclusion: The results of the study indicated that vitamin D related genetic variants were implicated in severe COVID-19 in adults. This could direct prevention strategies based on population specific nutrigenetic profiles.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Nutrition
T1  - Association of Vitamin D, Zinc and Selenium Related Genetic Variants With COVID-19 Disease Severity
VL  - 8
DO  - 10.3389/fnut.2021.689419
ER  - 

@article{
author = "Kotur, Nikola and Skakić, Anita and Klaassen, Kristel and Gašić, Vladimir and Zukić, Branka and Skodrić-Trifunović, Vesna and Stjepanović, Mihailo and Zivković, Zorica and Ostojić, Olivera and Stevanović, Goran and Lavadinović, Lidija and Pavlović, Sonja and Stanković, Biljana",
year = "2021",
abstract = "Background: COVID-19 pandemic has proved to be an unrelenting health threat for more than a year now. The emerging amount of data indicates that vitamin D, zinc and selenium could be important for clinical presentation of COVID-19. Here, we investigated association of genetic variants related to the altered level and bioavailability of vitamin D, zinc and selenium with clinical severity of COVID-19. Methods: We analyzed variants in genes significant for the status of vitamin D (DHCR7/NADSYN1 rs12785878, GC rs2282679, CYP2R1 rs10741657, and VDR rs2228570), zinc (PPCDC rs2120019) and selenium (DMGDH rs17823744) in 120 Serbian adult and pediatric COVID-19 patients using allelic discrimination. Furthermore, we carried out comparative population genetic analysis among European and other worldwide populations to investigate variation in allelic frequencies of selected variants. Results: Study showed that DHCR7/NADSYN rs12785878 and CYP2R1 rs10741657 variants were associated with severe COVID-19 in adults (p = 0.03, p = 0.017, respectively); carriers of DHCR7/NADSYN TG+GG and CYP2R1 GG genotypes had 0.21 and 5.9 the odds for developing severe disease, OR 0.21 (0.05-0.9) and OR 5.9 (1.4-25.2), respectively. There were no associations between selected genetic variants and disease severity in pediatric patients. Comparative population genetic analysis revealed that Serbian population had the lowest frequency of CYP2R1 rs10741657 G allele compared to other non-Finish Europeans (0.58 compared to 0.69 and 0.66 in Spanish and Italian population, respectively), suggesting that other populations should also investigate the relationship of CYP2R1 variant and the COVID-19 disease course. Conclusion: The results of the study indicated that vitamin D related genetic variants were implicated in severe COVID-19 in adults. This could direct prevention strategies based on population specific nutrigenetic profiles.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Nutrition",
title = "Association of Vitamin D, Zinc and Selenium Related Genetic Variants With COVID-19 Disease Severity",
volume = "8",
doi = "10.3389/fnut.2021.689419"
}

Kotur, N., Skakić, A., Klaassen, K., Gašić, V., Zukić, B., Skodrić-Trifunović, V., Stjepanović, M., Zivković, Z., Ostojić, O., Stevanović, G., Lavadinović, L., Pavlović, S.,& Stanković, B.. (2021). Association of Vitamin D, Zinc and Selenium Related Genetic Variants With COVID-19 Disease Severity. in Frontiers in Nutrition
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fnut.2021.689419

Kotur N, Skakić A, Klaassen K, Gašić V, Zukić B, Skodrić-Trifunović V, Stjepanović M, Zivković Z, Ostojić O, Stevanović G, Lavadinović L, Pavlović S, Stanković B. Association of Vitamin D, Zinc and Selenium Related Genetic Variants With COVID-19 Disease Severity. in Frontiers in Nutrition. 2021;8.
doi:10.3389/fnut.2021.689419 .

Kotur, Nikola, Skakić, Anita, Klaassen, Kristel, Gašić, Vladimir, Zukić, Branka, Skodrić-Trifunović, Vesna, Stjepanović, Mihailo, Zivković, Zorica, Ostojić, Olivera, Stevanović, Goran, Lavadinović, Lidija, Pavlović, Sonja, Stanković, Biljana, "Association of Vitamin D, Zinc and Selenium Related Genetic Variants With COVID-19 Disease Severity" in Frontiers in Nutrition, 8 (2021),
https://doi.org/10.3389/fnut.2021.689419 . .

TY  - JOUR
AU  - Srzentić Dražilov, Sanja
AU  - Nikčević, Gordana
AU  - Spasovski, Dusko
AU  - Bascarević, Zoran
AU  - Zivković, Zorica
AU  - Terzić-Supić, Zorica
AU  - Matanović, Dragana
AU  - Đorđević, Valentina
AU  - Pavlović, Sonja
AU  - Spasovski, Vesna
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/796
AB  - Perthes disease is one of the most common forms of pediatric femoral head osteonecrosis with an unknown etiology. Coagulation factors were the first genetic factors suspected to have a role in the pathogenesis of this disease, but studies showed inconsistent results. It is described that inflammation is present during early stages of Perthes disease, but its genetic aspect has not been studied extensively. Little is known regarding the status of apoptotic factors during the repair process that leads to the occurrence of hip deformity in patients. Therefore, the aim of this study was to analyze major mediators involved in coagulation, inflammation, and apoptotic processes as possible causative factors of Perthes disease. The study cohort consisted of 37 patients. Gene variants of TNF-alpha, FV, FII, and MTHFR genes were determined by PCR-RFLP, while IL-3 and PAI-1 were genotyped by direct sequencing. The expression level of Bax, Bcl-2, Bcl2L12, Fas and FasL was analyzed by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) technique. Our results showed a significantly increased level of expression of pro-apoptotic factor Bax along with significantly higher Bax/Bcl-2 ratio in the patient group. Conclusion: The results presented indicate that apoptosis could be one of the factors contributing to the lack of balanced bone remodeling process in Perthes patients.
PB  - Springer, New York
T2  - European Journal of Pediatrics
T1  - Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience
EP  - 1092
IS  - 8
SP  - 1085
VL  - 174
DO  - 10.1007/s00431-015-2510-z
ER  - 

@article{
author = "Srzentić Dražilov, Sanja and Nikčević, Gordana and Spasovski, Dusko and Bascarević, Zoran and Zivković, Zorica and Terzić-Supić, Zorica and Matanović, Dragana and Đorđević, Valentina and Pavlović, Sonja and Spasovski, Vesna",
year = "2015",
abstract = "Perthes disease is one of the most common forms of pediatric femoral head osteonecrosis with an unknown etiology. Coagulation factors were the first genetic factors suspected to have a role in the pathogenesis of this disease, but studies showed inconsistent results. It is described that inflammation is present during early stages of Perthes disease, but its genetic aspect has not been studied extensively. Little is known regarding the status of apoptotic factors during the repair process that leads to the occurrence of hip deformity in patients. Therefore, the aim of this study was to analyze major mediators involved in coagulation, inflammation, and apoptotic processes as possible causative factors of Perthes disease. The study cohort consisted of 37 patients. Gene variants of TNF-alpha, FV, FII, and MTHFR genes were determined by PCR-RFLP, while IL-3 and PAI-1 were genotyped by direct sequencing. The expression level of Bax, Bcl-2, Bcl2L12, Fas and FasL was analyzed by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) technique. Our results showed a significantly increased level of expression of pro-apoptotic factor Bax along with significantly higher Bax/Bcl-2 ratio in the patient group. Conclusion: The results presented indicate that apoptosis could be one of the factors contributing to the lack of balanced bone remodeling process in Perthes patients.",
publisher = "Springer, New York",
journal = "European Journal of Pediatrics",
title = "Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience",
pages = "1092-1085",
number = "8",
volume = "174",
doi = "10.1007/s00431-015-2510-z"
}

Srzentić Dražilov, S., Nikčević, G., Spasovski, D., Bascarević, Z., Zivković, Z., Terzić-Supić, Z., Matanović, D., Đorđević, V., Pavlović, S.,& Spasovski, V.. (2015). Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience. in European Journal of Pediatrics
Springer, New York., 174(8), 1085-1092.
https://doi.org/10.1007/s00431-015-2510-z

Srzentić Dražilov S, Nikčević G, Spasovski D, Bascarević Z, Zivković Z, Terzić-Supić Z, Matanović D, Đorđević V, Pavlović S, Spasovski V. Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience. in European Journal of Pediatrics. 2015;174(8):1085-1092.
doi:10.1007/s00431-015-2510-z .

Srzentić Dražilov, Sanja, Nikčević, Gordana, Spasovski, Dusko, Bascarević, Zoran, Zivković, Zorica, Terzić-Supić, Zorica, Matanović, Dragana, Đorđević, Valentina, Pavlović, Sonja, Spasovski, Vesna, "Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience" in European Journal of Pediatrics, 174, no. 8 (2015):1085-1092,
https://doi.org/10.1007/s00431-015-2510-z . .

TY  - JOUR
AU  - Vukasinović, Zoran
AU  - Spasovski, Dusko
AU  - Kralj-Iglić, Veronika
AU  - Marinković-Erić, Jelena
AU  - Seslija, Igor
AU  - Zivković, Zorica
AU  - Spasovski, Vesna
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/616
AB  - We studied changes of contact stress distribution in the hip joint after Tonnis triple pelvic osteotomy applied in the treatment of dysplasia and hip joint incongruence in adolescents. In a group of 75 patients, 54 (72 %) female, who underwent surgery by triple pelvic osteotomy in adolescence for developmental disorder of the hip and avascular necrosis of the femoral head, a three-dimensional hip joint model was used based on the radiography of the pelvis with hips. The following biomechanical parameters were calculated: resultant hip force normalised to body weight (R/Wb), inclination of the resultant hip force (theta-R), the position of the stress pole (theta), peak contact hip stress (Pmax), and peak contact hip stress normalised to body weight (Pmax/Wb). Gait quality was also assessed. After surgery the Wiberg CE angle was increased by 17.85A degrees (114 %), resultant hip force normalised to body weight (R/Wb) was decreased by 0.107 (3.3 %), the position of the stress pole was shifted medially by 27.59A degrees (63.5 %), and peak contact hip stress normalised to body weight (Pmax/Wb) was decreased by 2249.74 (55.9 %). Waddling gait was reduced from 17 (23.9 %) to four cases (5.6 %). All changes were statistically highly significant (p  lt  0.01). The effect of Tonnis triple pelvic osteotomy lies in the improvement of stress distribution across the acetabular cartilage of the hip joint, thus slowing down the degenerative damage of the hip joint.
PB  - Springer, New York
T2  - International Orthopaedics
T1  - Impact of triple pelvic osteotomy on contact stress pressure distribution in the hip joint
EP  - 98
IS  - 1
SP  - 95
VL  - 37
DO  - 10.1007/s00264-012-1727-y
ER  - 

@article{
author = "Vukasinović, Zoran and Spasovski, Dusko and Kralj-Iglić, Veronika and Marinković-Erić, Jelena and Seslija, Igor and Zivković, Zorica and Spasovski, Vesna",
year = "2013",
abstract = "We studied changes of contact stress distribution in the hip joint after Tonnis triple pelvic osteotomy applied in the treatment of dysplasia and hip joint incongruence in adolescents. In a group of 75 patients, 54 (72 %) female, who underwent surgery by triple pelvic osteotomy in adolescence for developmental disorder of the hip and avascular necrosis of the femoral head, a three-dimensional hip joint model was used based on the radiography of the pelvis with hips. The following biomechanical parameters were calculated: resultant hip force normalised to body weight (R/Wb), inclination of the resultant hip force (theta-R), the position of the stress pole (theta), peak contact hip stress (Pmax), and peak contact hip stress normalised to body weight (Pmax/Wb). Gait quality was also assessed. After surgery the Wiberg CE angle was increased by 17.85A degrees (114 %), resultant hip force normalised to body weight (R/Wb) was decreased by 0.107 (3.3 %), the position of the stress pole was shifted medially by 27.59A degrees (63.5 %), and peak contact hip stress normalised to body weight (Pmax/Wb) was decreased by 2249.74 (55.9 %). Waddling gait was reduced from 17 (23.9 %) to four cases (5.6 %). All changes were statistically highly significant (p  lt  0.01). The effect of Tonnis triple pelvic osteotomy lies in the improvement of stress distribution across the acetabular cartilage of the hip joint, thus slowing down the degenerative damage of the hip joint.",
publisher = "Springer, New York",
journal = "International Orthopaedics",
title = "Impact of triple pelvic osteotomy on contact stress pressure distribution in the hip joint",
pages = "98-95",
number = "1",
volume = "37",
doi = "10.1007/s00264-012-1727-y"
}

Vukasinović, Z., Spasovski, D., Kralj-Iglić, V., Marinković-Erić, J., Seslija, I., Zivković, Z.,& Spasovski, V.. (2013). Impact of triple pelvic osteotomy on contact stress pressure distribution in the hip joint. in International Orthopaedics
Springer, New York., 37(1), 95-98.
https://doi.org/10.1007/s00264-012-1727-y

Vukasinović Z, Spasovski D, Kralj-Iglić V, Marinković-Erić J, Seslija I, Zivković Z, Spasovski V. Impact of triple pelvic osteotomy on contact stress pressure distribution in the hip joint. in International Orthopaedics. 2013;37(1):95-98.
doi:10.1007/s00264-012-1727-y .

Vukasinović, Zoran, Spasovski, Dusko, Kralj-Iglić, Veronika, Marinković-Erić, Jelena, Seslija, Igor, Zivković, Zorica, Spasovski, Vesna, "Impact of triple pelvic osteotomy on contact stress pressure distribution in the hip joint" in International Orthopaedics, 37, no. 1 (2013):95-98,
https://doi.org/10.1007/s00264-012-1727-y . .