TY  - JOUR
AU  - Azanjac, Natalija
AU  - Milisavljević, Mira
AU  - Stanović, Stefan
AU  - Kojić, Milorad
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S1568786424000855
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2403
AB  - To identify new molecular components of the Brh2-governed homologous recombination (HR)-network in the highly radiation-resistant fungus Ustilago maydis, we undertook a genetic screen for suppressors of blm-KR hydroxyurea (HU)-sensitivity. Twenty DNA-damage sensitive mutants were obtained, three of which showing slow-growth phenotypes. Focusing on the “normally” growing candidates we identified five mutations, two in previously well-defined genes (Rec2 and Rad51) and the remaining three in completely uncharacterized genes (named Rec3, Bls9 and Zdr1). A common feature among these novel factors is their prominent role in DNA repair. Rec3 contains the P-loop NTPase domain which is most similar to that found in U. maydis Rec2 protein, and like Rec2, Rec3 plays critical roles in induced allelic recombination, is crucial for completion of meiosis, and with regard to DNA repair Δrec3 and Δrec2 are epistatic to one another. Importantly, overexpression of Brh2 in Δrec3 can effectively restore DNA-damage resistance, indicating a close functional connection between Brh2 and Rec3. The Bls9 does not seem to have any convincing domains that would give a clue as to its function. Nevertheless, we present evidence that, besides being involved in DNA-repair, Bls9 is also necessary for HR between chromosome homologs. Moreover, Δbls9 showed epistasis with Δbrh2 with respect to killing by DNA-damaging agents. Both, Rec3 and Bls9, play an important role in protecting the genome from mutations. Zdr1 is Cys2-His2 zinc finger (C2H2-ZF) protein, whose loss does not cause a detectable change in HR. Also, the functions of both Bls9 and Zdr1 genes are dispensable in meiosis and sporulation. However, Zdr1 appears to have overlapping activities with Blm and Mus81 in protecting the organism from methyl methanesulfonate- and diepoxybutane-induced DNA-damage. Finally, while deletion of Rec3 and Zdr1 can suppress HU-sensitivity of blm-KR, Δgen1, and Δmus81 mutants, interestingly loss of Bls9 does not rescue HU-sensitivity of Δgen1.
T2  - DNA Repair
T1  - Suppressors of Blm-deficiency identify three novel proteins that facilitate DNA repair in Ustilago maydis
SP  - 103709
VL  - 140
DO  - 10.1016/j.dnarep.2024.103709
ER  - 

@article{
author = "Azanjac, Natalija and Milisavljević, Mira and Stanović, Stefan and Kojić, Milorad",
year = "2024",
abstract = "To identify new molecular components of the Brh2-governed homologous recombination (HR)-network in the highly radiation-resistant fungus Ustilago maydis, we undertook a genetic screen for suppressors of blm-KR hydroxyurea (HU)-sensitivity. Twenty DNA-damage sensitive mutants were obtained, three of which showing slow-growth phenotypes. Focusing on the “normally” growing candidates we identified five mutations, two in previously well-defined genes (Rec2 and Rad51) and the remaining three in completely uncharacterized genes (named Rec3, Bls9 and Zdr1). A common feature among these novel factors is their prominent role in DNA repair. Rec3 contains the P-loop NTPase domain which is most similar to that found in U. maydis Rec2 protein, and like Rec2, Rec3 plays critical roles in induced allelic recombination, is crucial for completion of meiosis, and with regard to DNA repair Δrec3 and Δrec2 are epistatic to one another. Importantly, overexpression of Brh2 in Δrec3 can effectively restore DNA-damage resistance, indicating a close functional connection between Brh2 and Rec3. The Bls9 does not seem to have any convincing domains that would give a clue as to its function. Nevertheless, we present evidence that, besides being involved in DNA-repair, Bls9 is also necessary for HR between chromosome homologs. Moreover, Δbls9 showed epistasis with Δbrh2 with respect to killing by DNA-damaging agents. Both, Rec3 and Bls9, play an important role in protecting the genome from mutations. Zdr1 is Cys2-His2 zinc finger (C2H2-ZF) protein, whose loss does not cause a detectable change in HR. Also, the functions of both Bls9 and Zdr1 genes are dispensable in meiosis and sporulation. However, Zdr1 appears to have overlapping activities with Blm and Mus81 in protecting the organism from methyl methanesulfonate- and diepoxybutane-induced DNA-damage. Finally, while deletion of Rec3 and Zdr1 can suppress HU-sensitivity of blm-KR, Δgen1, and Δmus81 mutants, interestingly loss of Bls9 does not rescue HU-sensitivity of Δgen1.",
journal = "DNA Repair",
title = "Suppressors of Blm-deficiency identify three novel proteins that facilitate DNA repair in Ustilago maydis",
pages = "103709",
volume = "140",
doi = "10.1016/j.dnarep.2024.103709"
}

Azanjac, N., Milisavljević, M., Stanović, S.,& Kojić, M.. (2024). Suppressors of Blm-deficiency identify three novel proteins that facilitate DNA repair in Ustilago maydis. in DNA Repair, 140, 103709.
https://doi.org/10.1016/j.dnarep.2024.103709

Azanjac N, Milisavljević M, Stanović S, Kojić M. Suppressors of Blm-deficiency identify three novel proteins that facilitate DNA repair in Ustilago maydis. in DNA Repair. 2024;140:103709.
doi:10.1016/j.dnarep.2024.103709 .

Azanjac, Natalija, Milisavljević, Mira, Stanović, Stefan, Kojić, Milorad, "Suppressors of Blm-deficiency identify three novel proteins that facilitate DNA repair in Ustilago maydis" in DNA Repair, 140 (2024):103709,
https://doi.org/10.1016/j.dnarep.2024.103709 . .

TY  - JOUR
AU  - Nikolić, Ivana
AU  - Milisavljević, Mira
AU  - Timotijević, Gordana
PY  - 2024
UR  - https://www.mdpi.com/1422-0067/25/12/6291
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2399
AB  - Oxidative stress represents a critical facet of the array of abiotic stresses affecting crop growth and yield. In this paper, we investigated the potential differences in the functions of two highly homologous Arabidopsis DSS1 proteins in terms of maintaining genome integrity and response to oxidative stress. In the context of homologous recombination (HR), it was shown that overexpressing AtDSS1(I) using a functional complementation test increases the resistance of the Δdss1 mutant of Ustilago maydis to genotoxic agents. This indicates its conserved role in DNA repair via HR. To investigate the global transcriptome changes occurring in dss1 plant mutant lines, gene expression analysis was conducted using Illumina RNA sequencing technology. Individual RNA libraries were constructed from three total RNA samples isolated from dss1(I), dss1(V), and wild-type (WT) plants under hydrogen peroxide-induced stress. RNA-Seq data analysis and real-time PCR identification revealed major changes in gene expression between mutant lines and WT, while the dss1(I) and dss1(V) mutant lines exhibited analogous transcription profiles. The Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed significantly enriched metabolic pathways. Notably, genes associated with HR were upregulated in dss1 mutants compared to the WT. Otherwise, genes of the metabolic pathway responsible for the synthesis of secondary metabolites were downregulated in both dss1 mutant lines. These findings highlight the importance of understanding the molecular mechanisms of plant responses to oxidative stress.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Assessing Transcriptomic Responses to Oxidative Stress: Contrasting Wild-Type Arabidopsis Seedlings with dss1(I) and dss1(V) Gene Knockout Mutants
IS  - 12
SP  - 6291
VL  - 25
DO  - 10.3390/ijms25126291
ER  - 

@article{
author = "Nikolić, Ivana and Milisavljević, Mira and Timotijević, Gordana",
year = "2024",
abstract = "Oxidative stress represents a critical facet of the array of abiotic stresses affecting crop growth and yield. In this paper, we investigated the potential differences in the functions of two highly homologous Arabidopsis DSS1 proteins in terms of maintaining genome integrity and response to oxidative stress. In the context of homologous recombination (HR), it was shown that overexpressing AtDSS1(I) using a functional complementation test increases the resistance of the Δdss1 mutant of Ustilago maydis to genotoxic agents. This indicates its conserved role in DNA repair via HR. To investigate the global transcriptome changes occurring in dss1 plant mutant lines, gene expression analysis was conducted using Illumina RNA sequencing technology. Individual RNA libraries were constructed from three total RNA samples isolated from dss1(I), dss1(V), and wild-type (WT) plants under hydrogen peroxide-induced stress. RNA-Seq data analysis and real-time PCR identification revealed major changes in gene expression between mutant lines and WT, while the dss1(I) and dss1(V) mutant lines exhibited analogous transcription profiles. The Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed significantly enriched metabolic pathways. Notably, genes associated with HR were upregulated in dss1 mutants compared to the WT. Otherwise, genes of the metabolic pathway responsible for the synthesis of secondary metabolites were downregulated in both dss1 mutant lines. These findings highlight the importance of understanding the molecular mechanisms of plant responses to oxidative stress.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Assessing Transcriptomic Responses to Oxidative Stress: Contrasting Wild-Type Arabidopsis Seedlings with dss1(I) and dss1(V) Gene Knockout Mutants",
number = "12",
pages = "6291",
volume = "25",
doi = "10.3390/ijms25126291"
}

Nikolić, I., Milisavljević, M.,& Timotijević, G.. (2024). Assessing Transcriptomic Responses to Oxidative Stress: Contrasting Wild-Type Arabidopsis Seedlings with dss1(I) and dss1(V) Gene Knockout Mutants. in International Journal of Molecular Sciences
MDPI., 25(12), 6291.
https://doi.org/10.3390/ijms25126291

Nikolić I, Milisavljević M, Timotijević G. Assessing Transcriptomic Responses to Oxidative Stress: Contrasting Wild-Type Arabidopsis Seedlings with dss1(I) and dss1(V) Gene Knockout Mutants. in International Journal of Molecular Sciences. 2024;25(12):6291.
doi:10.3390/ijms25126291 .

Nikolić, Ivana, Milisavljević, Mira, Timotijević, Gordana, "Assessing Transcriptomic Responses to Oxidative Stress: Contrasting Wild-Type Arabidopsis Seedlings with dss1(I) and dss1(V) Gene Knockout Mutants" in International Journal of Molecular Sciences, 25, no. 12 (2024):6291,
https://doi.org/10.3390/ijms25126291 . .

TY  - JOUR
AU  - Adžić, Slađan
AU  - Pavlović, Nenad
AU  - Girek, Zdenka
AU  - Milisavljević, Mira
AU  - Ugrinović, Milan
AU  - Živković, Ivana
AU  - Đurić, Nenad
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2210
AB  - The expression of genes that induce the transformation of meristems into the reproductive stage in oilseed rape is realized inconditions of low positive temperatures for a certain period of time. Such a flowering process is called the vernalization pathway.A four-factor field trial with 6 genotypes of head cabbage was set up at the Institute of Vegetable Crops in Smederevska Palanka,of which three parental genotypes were divergent by geographical origin: Scc, B and N, and three more F1 hybrids were selectedby diallel crossing: Scc x B, Scc x N and B x N. In order to achieve a different vegetative stage, seedlings were sown at three sowingdates: August 15th, September 1st and September 15th. Transplanting was done on October 20th. The results of sowing headcabbage within the sowing period for oilseed rape were the induction of the flower mechanism, the absence of the head formationphenophase, and the realization of a stable seed yield. The experiment was performed in vivo in the control version and in thetreatment with gibberellic acid – GA3. The influence of all four factors: season, genotype, sowing date and GA3 treatment showedstatistical significance for the yield components as well as for the yield itself and seed quality. The three seasons in which theexperiment was evaluated differed in temperature during overwintering: 2010/2011 was moderately cold, 2011/2012 wasextremely cold, while 2012/2013 was warm. In the cold season, the seed yield was low, and reduced to the biologicalmaintenance of the species, while the highest seed yield was achieved in the third – warm (2012/2013) season in the first sowingperiod. The experiment also confirmed the existence of an identical flower mechanism in the species Brassica napus L. andBrassica oleracea var. capitata L.
AB  - Експресија гена који индукују трансформацију меристема у репродуктивни стадијум код уљане репице остварује се уусловима ниских позитивних температура у извесном трајању. Такав процес цветања назива се вернализациони пут. УИнституту за повртарство из Смедеревске Паланке постављен је четворофакторски пољски оглед са 6 генотипова купусаглавичара, од којих су три родитељска генотипа дивергентна по географском пореклу: Scc, B и N, а од њих је диалелнимукрштањем селекционисано још три F1 хибрида: Scc x B, Scc x N, и B x N. У циљу постизања различитог вегетативногстадијума расада, сетва је вршена у три рока сетве: 15. август, 1. септембар и 15. септембар. Расађивање је вршено 20.октобра. Резултат сетве купуса главичара у року сетве за уљану репицу била је индукција цветног механизма, изостанакфенофазе формирања главице и реализација стабилног приноса семена. Оглед је постављен in vivo у контролној верзијии у третману гиберелинском киселином – GA3. Утицај сва четири фактора: сезоне, генотипа, рока сетве и третмана GA3показао је статистичку значајност на особине компоненте приноса, као и сам принос и квалитет семена. Три сезоне укојима је оглед евалуиран биле су температурно различите у периоду презимљавања: 2010/2011 је била умеренохладна, 2011/2012 је била изразито хладна, док је 2012/2013 била топла. У температурно хладној сезони принос семенабио је низак, сведен на биолошко одржавање врсте, док је највећи принос семена остварен у трећој – топлој (2012/2013)сезони истраживања у првом року сетве. Оглед је потврдио и постојање идентичног цветног механизма код врстаBrassica napus L. и Brassica oleracea var. capitata L.Кључне речи: купус главичар, уљана репица, вернализација
PB  - Univerzitet u Kragujevcu Agronomski fakultet u Čačku
T2  - Acta Agriculturae Serbica
T1  - Cabbage (Brassica oleracea var. capitata L.) grown under the conditions of the life cycle of winter oilseed rape (Brassica napus L.) in order to achieve a stable seed yield
EP  - 12
IS  - 55
SP  - 3
VL  - 28
DO  - 10.5937/AASer2355003A
ER  - 

@article{
author = "Adžić, Slađan and Pavlović, Nenad and Girek, Zdenka and Milisavljević, Mira and Ugrinović, Milan and Živković, Ivana and Đurić, Nenad",
year = "2023",
abstract = "The expression of genes that induce the transformation of meristems into the reproductive stage in oilseed rape is realized inconditions of low positive temperatures for a certain period of time. Such a flowering process is called the vernalization pathway.A four-factor field trial with 6 genotypes of head cabbage was set up at the Institute of Vegetable Crops in Smederevska Palanka,of which three parental genotypes were divergent by geographical origin: Scc, B and N, and three more F1 hybrids were selectedby diallel crossing: Scc x B, Scc x N and B x N. In order to achieve a different vegetative stage, seedlings were sown at three sowingdates: August 15th, September 1st and September 15th. Transplanting was done on October 20th. The results of sowing headcabbage within the sowing period for oilseed rape were the induction of the flower mechanism, the absence of the head formationphenophase, and the realization of a stable seed yield. The experiment was performed in vivo in the control version and in thetreatment with gibberellic acid – GA3. The influence of all four factors: season, genotype, sowing date and GA3 treatment showedstatistical significance for the yield components as well as for the yield itself and seed quality. The three seasons in which theexperiment was evaluated differed in temperature during overwintering: 2010/2011 was moderately cold, 2011/2012 wasextremely cold, while 2012/2013 was warm. In the cold season, the seed yield was low, and reduced to the biologicalmaintenance of the species, while the highest seed yield was achieved in the third – warm (2012/2013) season in the first sowingperiod. The experiment also confirmed the existence of an identical flower mechanism in the species Brassica napus L. andBrassica oleracea var. capitata L., Експресија гена који индукују трансформацију меристема у репродуктивни стадијум код уљане репице остварује се уусловима ниских позитивних температура у извесном трајању. Такав процес цветања назива се вернализациони пут. УИнституту за повртарство из Смедеревске Паланке постављен је четворофакторски пољски оглед са 6 генотипова купусаглавичара, од којих су три родитељска генотипа дивергентна по географском пореклу: Scc, B и N, а од њих је диалелнимукрштањем селекционисано још три F1 хибрида: Scc x B, Scc x N, и B x N. У циљу постизања различитог вегетативногстадијума расада, сетва је вршена у три рока сетве: 15. август, 1. септембар и 15. септембар. Расађивање је вршено 20.октобра. Резултат сетве купуса главичара у року сетве за уљану репицу била је индукција цветног механизма, изостанакфенофазе формирања главице и реализација стабилног приноса семена. Оглед је постављен in vivo у контролној верзијии у третману гиберелинском киселином – GA3. Утицај сва четири фактора: сезоне, генотипа, рока сетве и третмана GA3показао је статистичку значајност на особине компоненте приноса, као и сам принос и квалитет семена. Три сезоне укојима је оглед евалуиран биле су температурно различите у периоду презимљавања: 2010/2011 је била умеренохладна, 2011/2012 је била изразито хладна, док је 2012/2013 била топла. У температурно хладној сезони принос семенабио је низак, сведен на биолошко одржавање врсте, док је највећи принос семена остварен у трећој – топлој (2012/2013)сезони истраживања у првом року сетве. Оглед је потврдио и постојање идентичног цветног механизма код врстаBrassica napus L. и Brassica oleracea var. capitata L.Кључне речи: купус главичар, уљана репица, вернализација",
publisher = "Univerzitet u Kragujevcu Agronomski fakultet u Čačku",
journal = "Acta Agriculturae Serbica",
title = "Cabbage (Brassica oleracea var. capitata L.) grown under the conditions of the life cycle of winter oilseed rape (Brassica napus L.) in order to achieve a stable seed yield",
pages = "12-3",
number = "55",
volume = "28",
doi = "10.5937/AASer2355003A"
}

Adžić, S., Pavlović, N., Girek, Z., Milisavljević, M., Ugrinović, M., Živković, I.,& Đurić, N.. (2023). Cabbage (Brassica oleracea var. capitata L.) grown under the conditions of the life cycle of winter oilseed rape (Brassica napus L.) in order to achieve a stable seed yield. in Acta Agriculturae Serbica
Univerzitet u Kragujevcu Agronomski fakultet u Čačku., 28(55), 3-12.
https://doi.org/10.5937/AASer2355003A

Adžić S, Pavlović N, Girek Z, Milisavljević M, Ugrinović M, Živković I, Đurić N. Cabbage (Brassica oleracea var. capitata L.) grown under the conditions of the life cycle of winter oilseed rape (Brassica napus L.) in order to achieve a stable seed yield. in Acta Agriculturae Serbica. 2023;28(55):3-12.
doi:10.5937/AASer2355003A .

Adžić, Slađan, Pavlović, Nenad, Girek, Zdenka, Milisavljević, Mira, Ugrinović, Milan, Živković, Ivana, Đurić, Nenad, "Cabbage (Brassica oleracea var. capitata L.) grown under the conditions of the life cycle of winter oilseed rape (Brassica napus L.) in order to achieve a stable seed yield" in Acta Agriculturae Serbica, 28, no. 55 (2023):3-12,
https://doi.org/10.5937/AASer2355003A . .

TY  - JOUR
AU  - Nikolić, Ivana
AU  - Samardžić, Jelena
AU  - Stevanović, Strahinja
AU  - Miljuš-Đukić, Jovanka
AU  - Milisavljević, Mira
AU  - Timotijević, Gordana
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/3/2442
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1883
AB  - Global climate change has a detrimental effect on plant growth and health, causing serious losses in agriculture. Investigation of the molecular mechanisms of plant responses to various environmental pressures and the generation of plants tolerant to abiotic stress are imperative to modern plant science. In this paper, we focus on the application of the well-established technology CRISPR/Cas9 genome editing to better understand the functioning of the intrinsically disordered protein DSS1 in plant response to oxidative stress. The Arabidopsis genome contains two highly homologous DSS1 genes, AtDSS1(I) and AtDSS1(V). This study was designed to identify the functional differences between AtDSS1s, focusing on their potential roles in oxidative stress. We generated single dss1(I) and dss1(V) mutant lines of both Arabidopsis DSS1 genes using CRISPR/Cas9 technology. The homozygous mutant lines with large indels (dss1(I)del25 and dss1(V)ins18) were phenotypically characterized during plant development and their sensitivity to oxidative stress was analyzed. The characterization of mutant lines revealed differences in root and stem lengths, and rosette area size. Plants with a disrupted AtDSS1(V) gene exhibited lower survival rates and increased levels of oxidized proteins in comparison to WT plants exposed to oxidative stress induced by hydrogen peroxide. In this work, the dss1 double mutant was not obtained due to embryonic lethality. These results suggest that the DSS1(V) protein could be an important molecular component in plant abiotic stress response
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - CRISPR/Cas9-Targeted Disruption of Two Highly Homologous Arabidopsis thaliana DSS1 Genes with Roles in Development and the Oxidative Stress Response
IS  - 3
SP  - 2442
VL  - 24
DO  - 10.3390/ijms24032442
ER  - 

@article{
author = "Nikolić, Ivana and Samardžić, Jelena and Stevanović, Strahinja and Miljuš-Đukić, Jovanka and Milisavljević, Mira and Timotijević, Gordana",
year = "2023",
abstract = "Global climate change has a detrimental effect on plant growth and health, causing serious losses in agriculture. Investigation of the molecular mechanisms of plant responses to various environmental pressures and the generation of plants tolerant to abiotic stress are imperative to modern plant science. In this paper, we focus on the application of the well-established technology CRISPR/Cas9 genome editing to better understand the functioning of the intrinsically disordered protein DSS1 in plant response to oxidative stress. The Arabidopsis genome contains two highly homologous DSS1 genes, AtDSS1(I) and AtDSS1(V). This study was designed to identify the functional differences between AtDSS1s, focusing on their potential roles in oxidative stress. We generated single dss1(I) and dss1(V) mutant lines of both Arabidopsis DSS1 genes using CRISPR/Cas9 technology. The homozygous mutant lines with large indels (dss1(I)del25 and dss1(V)ins18) were phenotypically characterized during plant development and their sensitivity to oxidative stress was analyzed. The characterization of mutant lines revealed differences in root and stem lengths, and rosette area size. Plants with a disrupted AtDSS1(V) gene exhibited lower survival rates and increased levels of oxidized proteins in comparison to WT plants exposed to oxidative stress induced by hydrogen peroxide. In this work, the dss1 double mutant was not obtained due to embryonic lethality. These results suggest that the DSS1(V) protein could be an important molecular component in plant abiotic stress response",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "CRISPR/Cas9-Targeted Disruption of Two Highly Homologous Arabidopsis thaliana DSS1 Genes with Roles in Development and the Oxidative Stress Response",
number = "3",
pages = "2442",
volume = "24",
doi = "10.3390/ijms24032442"
}

Nikolić, I., Samardžić, J., Stevanović, S., Miljuš-Đukić, J., Milisavljević, M.,& Timotijević, G.. (2023). CRISPR/Cas9-Targeted Disruption of Two Highly Homologous Arabidopsis thaliana DSS1 Genes with Roles in Development and the Oxidative Stress Response. in International Journal of Molecular Sciences, 24(3), 2442.
https://doi.org/10.3390/ijms24032442

Nikolić I, Samardžić J, Stevanović S, Miljuš-Đukić J, Milisavljević M, Timotijević G. CRISPR/Cas9-Targeted Disruption of Two Highly Homologous Arabidopsis thaliana DSS1 Genes with Roles in Development and the Oxidative Stress Response. in International Journal of Molecular Sciences. 2023;24(3):2442.
doi:10.3390/ijms24032442 .

Nikolić, Ivana, Samardžić, Jelena, Stevanović, Strahinja, Miljuš-Đukić, Jovanka, Milisavljević, Mira, Timotijević, Gordana, "CRISPR/Cas9-Targeted Disruption of Two Highly Homologous Arabidopsis thaliana DSS1 Genes with Roles in Development and the Oxidative Stress Response" in International Journal of Molecular Sciences, 24, no. 3 (2023):2442,
https://doi.org/10.3390/ijms24032442 . .

TY  - CONF
AU  - Azanjac, Natalija
AU  - Kojić, Milorad
AU  - Milisavljević, Mira
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2161
AB  - Homologous recombination (HR) is one of the most accurate mechanisms of preserving genome
integrity by precise repair of double strand breaks as the most deleterious type of DNA damage.
Mechanisms of HR are mostly studied in yeast which, unlike higher eukaryotes employs RAD52 as a
HR mediator, instead of BRCA2.
Ustilago maydis is a unicellular phytopathogen characterized by extreme radiation resistance
dependent on BRCA2-driven HR. The focus of our research is to uncover novel cellular factors that
regulate HR, by isolating suppressors of blm in U. maydis.
We have identified 3 new factors of unknown functions, as well as Rad55 and Mph. All mutations
suppress HU sensitivity of blm. Presence of truncated proteins caused by point mutations that
introduce the specific premature STOP codon and complete deletions of UMAG_01566 and
UMAG_01667, both lead to genotoxins sensitivity and altered growth rates on HU to a various extent.
Mutation in UMAG_03150 leads to slow growth which can be suppressed by truncated UMAG_01566.
Mitotic or meiotic recombination is also affected in some of the mutants.
We assume that these novel factors can provide insights into HR regulation, interactions among HR
participants and relations to other cellular processes.
PB  - Francis Crick Institute
C3  - Fusion Conferences
T1  - NOVEL CELLULAR FACTORS INVOLVED IN REGULATION OF BRCA2-DRIVEN HOMOLOGOUS RECOMBINATION IN USTILAGO MAYDIS
EP  - 89
SP  - 89
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2161
ER  - 

@conference{
author = "Azanjac, Natalija and Kojić, Milorad and Milisavljević, Mira",
year = "2023",
abstract = "Homologous recombination (HR) is one of the most accurate mechanisms of preserving genome
integrity by precise repair of double strand breaks as the most deleterious type of DNA damage.
Mechanisms of HR are mostly studied in yeast which, unlike higher eukaryotes employs RAD52 as a
HR mediator, instead of BRCA2.
Ustilago maydis is a unicellular phytopathogen characterized by extreme radiation resistance
dependent on BRCA2-driven HR. The focus of our research is to uncover novel cellular factors that
regulate HR, by isolating suppressors of blm in U. maydis.
We have identified 3 new factors of unknown functions, as well as Rad55 and Mph. All mutations
suppress HU sensitivity of blm. Presence of truncated proteins caused by point mutations that
introduce the specific premature STOP codon and complete deletions of UMAG_01566 and
UMAG_01667, both lead to genotoxins sensitivity and altered growth rates on HU to a various extent.
Mutation in UMAG_03150 leads to slow growth which can be suppressed by truncated UMAG_01566.
Mitotic or meiotic recombination is also affected in some of the mutants.
We assume that these novel factors can provide insights into HR regulation, interactions among HR
participants and relations to other cellular processes.",
publisher = "Francis Crick Institute",
journal = "Fusion Conferences",
title = "NOVEL CELLULAR FACTORS INVOLVED IN REGULATION OF BRCA2-DRIVEN HOMOLOGOUS RECOMBINATION IN USTILAGO MAYDIS",
pages = "89-89",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2161"
}

Azanjac, N., Kojić, M.,& Milisavljević, M.. (2023). NOVEL CELLULAR FACTORS INVOLVED IN REGULATION OF BRCA2-DRIVEN HOMOLOGOUS RECOMBINATION IN USTILAGO MAYDIS. in Fusion Conferences
Francis Crick Institute., 89-89.
https://hdl.handle.net/21.15107/rcub_imagine_2161

Azanjac N, Kojić M, Milisavljević M. NOVEL CELLULAR FACTORS INVOLVED IN REGULATION OF BRCA2-DRIVEN HOMOLOGOUS RECOMBINATION IN USTILAGO MAYDIS. in Fusion Conferences. 2023;:89-89.
https://hdl.handle.net/21.15107/rcub_imagine_2161 .

Azanjac, Natalija, Kojić, Milorad, Milisavljević, Mira, "NOVEL CELLULAR FACTORS INVOLVED IN REGULATION OF BRCA2-DRIVEN HOMOLOGOUS RECOMBINATION IN USTILAGO MAYDIS" in Fusion Conferences (2023):89-89,
https://hdl.handle.net/21.15107/rcub_imagine_2161 .

TY  - CONF
AU  - Nikolić, Ivana
AU  - Timotijević, Gordana
AU  - Samardžić, Jelena
AU  - Milisavljević, Mira
PY  - 2023
UR  - https://europlantbiology2023.org/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1972
AB  - DSS1 (deletion of split hand/split foot 1) is a highly conserved, eukaryotic, and multifunctional
protein. DSS1 as a small intrinsically disordered protein binds to multiple proteins when it
gains a final conformation. There are two highly homologous genes, DSS1(I) and DSS1(V) in the
Arabidopsis genome. Our aim is to examine Atdss1 mutants through oxidative stress. We
obtained separate stable lines of Arabidopsis containing mutations in DSS1s using
CRISPR/Cas9 technology. After H2O2 treatment, mutant seedlings showed increased
sensitivity to oxidative stress in comparison to WT plants. Transcriptome analysis showed
that dss1(I)del25 and dss1(V)ins18 mutations caused 2762 and 2335 differentially expressed
genes compared to WT under oxidative stress, respectively. We found that upregulated
expression was in genes involved in homologue recombination and RNA transport in both dss1
lines. The most downregulated genes are classified into flavonoid biosynthesis and MAPK
signaling pathway.
PB  - Plant Biology Europe
C3  - 14th International Conference of the French Society of Plant Biology
T1  - Transcriptome analysis of Atdss1 mutants in response to oxidative stress
EP  - 172
SP  - 172
VL  - 14
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1972
ER  - 

@conference{
author = "Nikolić, Ivana and Timotijević, Gordana and Samardžić, Jelena and Milisavljević, Mira",
year = "2023",
abstract = "DSS1 (deletion of split hand/split foot 1) is a highly conserved, eukaryotic, and multifunctional
protein. DSS1 as a small intrinsically disordered protein binds to multiple proteins when it
gains a final conformation. There are two highly homologous genes, DSS1(I) and DSS1(V) in the
Arabidopsis genome. Our aim is to examine Atdss1 mutants through oxidative stress. We
obtained separate stable lines of Arabidopsis containing mutations in DSS1s using
CRISPR/Cas9 technology. After H2O2 treatment, mutant seedlings showed increased
sensitivity to oxidative stress in comparison to WT plants. Transcriptome analysis showed
that dss1(I)del25 and dss1(V)ins18 mutations caused 2762 and 2335 differentially expressed
genes compared to WT under oxidative stress, respectively. We found that upregulated
expression was in genes involved in homologue recombination and RNA transport in both dss1
lines. The most downregulated genes are classified into flavonoid biosynthesis and MAPK
signaling pathway.",
publisher = "Plant Biology Europe",
journal = "14th International Conference of the French Society of Plant Biology",
title = "Transcriptome analysis of Atdss1 mutants in response to oxidative stress",
pages = "172-172",
volume = "14",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1972"
}

Nikolić, I., Timotijević, G., Samardžić, J.,& Milisavljević, M.. (2023). Transcriptome analysis of Atdss1 mutants in response to oxidative stress. in 14th International Conference of the French Society of Plant Biology
Plant Biology Europe., 14, 172-172.
https://hdl.handle.net/21.15107/rcub_imagine_1972

Nikolić I, Timotijević G, Samardžić J, Milisavljević M. Transcriptome analysis of Atdss1 mutants in response to oxidative stress. in 14th International Conference of the French Society of Plant Biology. 2023;14:172-172.
https://hdl.handle.net/21.15107/rcub_imagine_1972 .

Nikolić, Ivana, Timotijević, Gordana, Samardžić, Jelena, Milisavljević, Mira, "Transcriptome analysis of Atdss1 mutants in response to oxidative stress" in 14th International Conference of the French Society of Plant Biology, 14 (2023):172-172,
https://hdl.handle.net/21.15107/rcub_imagine_1972 .

TY  - CONF
AU  - Azanjac, Natalija
AU  - Kojić, Milorad
AU  - Milisavljević, Mira
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1750
AB  - Održavanje stabilnosti genoma je jedna od najvažnijih ćelijskih funkcija, koja
joj omogućava preživljavanje i deobu. Jedan od mehanizama popravke DNK je
homologna rekombinacija (HR), čija mašinerija omogućava preciznu popravku
dvolančanih prekida, kao najštetnijih oštećenja DNK, te narušavanje HR može
dovesti do genomske nestabilnosti, koja je u osnovi čitavog niza bolesti. Uprkos
opsežnim istraživanjima HR na različitim organizmima i dalje nisu detaljno
okarakterisane sve funkcije poznatih HR-faktora, a otkrivaju se i novi faktori
uključeni u ovaj proces. Kao pogodan model sistem za pronalaženje novih
ćelijskih faktora HR-e, koristimo jednoćelijsku bazidiomicetu Ustilago maydis,
koja je visoko rezistentna na zračenje, poseduje osnovnu HR-mašineriju sličnu
humanoj, sa konzerviranim BRCA2 ortologom (Brh2)1 i ima 30% gena nepoznate
funkcije. Dizajniran je specifični molekularno-genetički pristup – kroz
izolovanje supresora mutanta blm, što zapravo znači izolovanje funkcionalnih
partnera Blm helikaze, čija je aktivnost sinhronizovana sa procesom popravke
DNK putem HR.2 Do sada su izolovana tri nova supresora (bls2, bls9 i bls10),
generisani su pojedinačni mutanti i okarakterisan je njihov fenotip.
Identifikovani geni kodiraju hipotetičke proteine. Bls9 i bls10 su izrazito
senzitivni na genotoksične agense i imaju narušenu efikasnost HR, dok bls2 ima
usporen rast. Detaljnom molekularno-genetičkom i biohemijskom analizom će se
definisati uloge ovih faktora u HR.
AB  - Одржавање стабилности генома је једна од најважнијих ћелијских функција, која
јој омогућава преживљавање и деобу. Један од механизама поправке ДНК је
хомологна рекомбинација (ХР), чија машинерија омогућава прецизну поправку
дволанчаних прекида, као најштетнијих оштећења ДНК, те нарушавање ХР може
довести до геномске нестабилности, која је у основи читавог низа болести. Упркос
опсежним истраживањима ХР на различитим организмима и даље нису детаљно
окарактерисане све функције познатих ХР-фактора, а откривају се и нови фактори
укључени у овај процес. Као погодан модел систем за проналажење нових
ћелијских фактора ХР-е, користимо једноћелијску базидиомицету Ustilago maydis,
која је високо резистентна на зрачење, поседује основну ХР-машинерију сличну
хуманој, са конзервираним BRCA2 ортологом (Brh2)1 и има 30% гена непознате
функције. Дизајниран је специфични молекуларно-генетички приступ – кроз
изоловање супресора мутанта blm, што заправо значи изоловање функционалних
партнера Blm хеликазе, чија је активност синхронизована са процесом поправке
ДНК путем ХР.2 До сада су изолована три нова супресора (bls2, bls9 и bls10),
генерисани су појединачни мутанти и окарактерисан је њихов фенотип.
Идентификовани гени кодирају хипотетичке протеине. Bls9 и bls10 су изразито
сензитивни на генотоксичне агенсе и имају нарушену ефикасност ХР, док bls2 има
успорен раст. Детаљном молекуларно-генетичком и биохемијском анализом ће се
дефинисати улоге ових фактора у ХР.
PB  - Beograd : Srpsko biološko društvo
C3  - Treći kongres biologa Srbije
T1  - Novi ćelijski faktori u regulaciji BRCA2-zavisne rekombinacije u Ustilago maydis
T1  - Нови ћелијски фактори у регулацији BRCA2-зависне рекомбинације у Ustilago maydis
SP  - 269
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1750
ER  - 

@conference{
author = "Azanjac, Natalija and Kojić, Milorad and Milisavljević, Mira",
year = "2022",
abstract = "Održavanje stabilnosti genoma je jedna od najvažnijih ćelijskih funkcija, koja
joj omogućava preživljavanje i deobu. Jedan od mehanizama popravke DNK je
homologna rekombinacija (HR), čija mašinerija omogućava preciznu popravku
dvolančanih prekida, kao najštetnijih oštećenja DNK, te narušavanje HR može
dovesti do genomske nestabilnosti, koja je u osnovi čitavog niza bolesti. Uprkos
opsežnim istraživanjima HR na različitim organizmima i dalje nisu detaljno
okarakterisane sve funkcije poznatih HR-faktora, a otkrivaju se i novi faktori
uključeni u ovaj proces. Kao pogodan model sistem za pronalaženje novih
ćelijskih faktora HR-e, koristimo jednoćelijsku bazidiomicetu Ustilago maydis,
koja je visoko rezistentna na zračenje, poseduje osnovnu HR-mašineriju sličnu
humanoj, sa konzerviranim BRCA2 ortologom (Brh2)1 i ima 30% gena nepoznate
funkcije. Dizajniran je specifični molekularno-genetički pristup – kroz
izolovanje supresora mutanta blm, što zapravo znači izolovanje funkcionalnih
partnera Blm helikaze, čija je aktivnost sinhronizovana sa procesom popravke
DNK putem HR.2 Do sada su izolovana tri nova supresora (bls2, bls9 i bls10),
generisani su pojedinačni mutanti i okarakterisan je njihov fenotip.
Identifikovani geni kodiraju hipotetičke proteine. Bls9 i bls10 su izrazito
senzitivni na genotoksične agense i imaju narušenu efikasnost HR, dok bls2 ima
usporen rast. Detaljnom molekularno-genetičkom i biohemijskom analizom će se
definisati uloge ovih faktora u HR., Одржавање стабилности генома је једна од најважнијих ћелијских функција, која
јој омогућава преживљавање и деобу. Један од механизама поправке ДНК је
хомологна рекомбинација (ХР), чија машинерија омогућава прецизну поправку
дволанчаних прекида, као најштетнијих оштећења ДНК, те нарушавање ХР може
довести до геномске нестабилности, која је у основи читавог низа болести. Упркос
опсежним истраживањима ХР на различитим организмима и даље нису детаљно
окарактерисане све функције познатих ХР-фактора, а откривају се и нови фактори
укључени у овај процес. Као погодан модел систем за проналажење нових
ћелијских фактора ХР-е, користимо једноћелијску базидиомицету Ustilago maydis,
која је високо резистентна на зрачење, поседује основну ХР-машинерију сличну
хуманој, са конзервираним BRCA2 ортологом (Brh2)1 и има 30% гена непознате
функције. Дизајниран је специфични молекуларно-генетички приступ – кроз
изоловање супресора мутанта blm, што заправо значи изоловање функционалних
партнера Blm хеликазе, чија је активност синхронизована са процесом поправке
ДНК путем ХР.2 До сада су изолована три нова супресора (bls2, bls9 и bls10),
генерисани су појединачни мутанти и окарактерисан је њихов фенотип.
Идентификовани гени кодирају хипотетичке протеине. Bls9 и bls10 су изразито
сензитивни на генотоксичне агенсе и имају нарушену ефикасност ХР, док bls2 има
успорен раст. Детаљном молекуларно-генетичком и биохемијском анализом ће се
дефинисати улоге ових фактора у ХР.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Treći kongres biologa Srbije",
title = "Novi ćelijski faktori u regulaciji BRCA2-zavisne rekombinacije u Ustilago maydis, Нови ћелијски фактори у регулацији BRCA2-зависне рекомбинације у Ustilago maydis",
pages = "269",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1750"
}

Azanjac, N., Kojić, M.,& Milisavljević, M.. (2022). Novi ćelijski faktori u regulaciji BRCA2-zavisne rekombinacije u Ustilago maydis. in Treći kongres biologa Srbije
Beograd : Srpsko biološko društvo., 269.
https://hdl.handle.net/21.15107/rcub_imagine_1750

Azanjac N, Kojić M, Milisavljević M. Novi ćelijski faktori u regulaciji BRCA2-zavisne rekombinacije u Ustilago maydis. in Treći kongres biologa Srbije. 2022;:269.
https://hdl.handle.net/21.15107/rcub_imagine_1750 .

Azanjac, Natalija, Kojić, Milorad, Milisavljević, Mira, "Novi ćelijski faktori u regulaciji BRCA2-zavisne rekombinacije u Ustilago maydis" in Treći kongres biologa Srbije (2022):269,
https://hdl.handle.net/21.15107/rcub_imagine_1750 .

TY  - CONF
AU  - Stanovčić, Stefan
AU  - Malešević, Jelena
AU  - Milisavljević, Mira
AU  - Kojić, Milorad
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1749
AB  - Promenljivost životne sredine je konstantna, te su živa bića morala razviti
različite strategije odgovora na nepovoljne sredinske uslove. Ove strategije se
mogu svrstati u dve glavne grupe: a) mehanizmi rezistencije/tolerancije i b)
mehanizmi obnove nakon jakog stresa. Efikasan mehanizam obnove populacije
nakon snažnog oksidativnog stresa je okarakterisan u jednoćelijskoj
bazidiomiceti, Ustilago maydis i zasniva se na proliferaciji preživelih ćelija
na račun biomolekula oslobođenih iz oštećenih i umrlih ćelija.1 Fenomen je
nazvan RUS (od engl. Repopulation under starvation, RUS) i do sada je
identifikovano 10 ćelijskih faktora uključenih u ovaj proces. Ova gljiva se
karakteriše i visokim stepenom otpornosti na UV i jonizujuće zračenje u čijoj
osnovi leži efikasna popravka DNK oštećenja. Interesantno je da su neki od
otkrivenih RUS faktora uključeni i u proces zaštite integriteta genoma.2 U
cilju otkrivanja novih faktora čija je funkcija vezana i za obnovu populacije i za
stabilnost genoma, primenjena su dva metodološka pristupa: pretraživanje
mutanata i analiza transkriptoma. Ovim pristupima je identifikovano 15
kandidata, a preliminarna analiza je potvrdila da su ovi ćelijski faktori
neophodni za oba procesa. Za dalji rad su selektovani kandidati sa
najizraženijim fenotipom i kroz molekularno-genetičke i biohemijske analize
će biti detaljnije okarakterisane njihove ćelijske funkcije.
AB  - Променљивост животне средине је константна, те су жива бића морала развити
различите стратегије одговора на неповољне срединске услове. Ове стратегије се
могу сврстати у две главне групе: а) механизми резистенције/толеранције и б)
механизми обнове након јаког стреса. Ефикасан механизам обнове популације
након снажног оксидативног стреса је окарактерисан у једноћелијској
базидиомицети, Ustilago maydis и заснива се на пролиферацији преживелих ћелија
на рачун биомолекула ослобођених из оштећених и умрлих ћелија.1 Феномен је
назван RUS (од енгл. Repopulation under starvation, RUS) и до сада је
идентификовано 10 ћелијских фактора укључених у овај процес. Ова гљива се
карактерише и високим степеном отпорности на УВ и јонизујуће зрачење у чијој
основи лежи ефикасна поправка ДНК оштећења. Интересантно је да су неки од
откривених РУС фактора укључени и у процес заштите интегритета генома.2 У
циљу откривања нових фактора чија је функција везана и за обнову популације и за
стабилност генома, примењена су два методолошка приступа: претраживање
мутаната и анализа транскриптома. Овим приступима је идентификовано 15
кандидата, а прелиминарна анализа је потврдила да су ови ћелијски фактори
неопходни за оба процеса. За даљи рад су селектовани кандидати са
најизраженијим фенотипом и кроз молекуларно-генетичке и биохемијске анализе
ће бити детаљније окарактерисане њихове ћелијске функције.
PB  - Beograd : Srpsko biološko društvo
C3  - Treći kongres biologa Srbije
T1  - Obnova ćelijske populacije Ustilago maydis nakon stresa i veza sa zaštitom genoma – novi ćelijski faktori
T1  - Обнова ћелијске популације Ustilago maydis након стреса и веза са заштитом генома – нови ћелијски фактори
SP  - 272
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1749
ER  - 

@conference{
author = "Stanovčić, Stefan and Malešević, Jelena and Milisavljević, Mira and Kojić, Milorad",
year = "2022",
abstract = "Promenljivost životne sredine je konstantna, te su živa bića morala razviti
različite strategije odgovora na nepovoljne sredinske uslove. Ove strategije se
mogu svrstati u dve glavne grupe: a) mehanizmi rezistencije/tolerancije i b)
mehanizmi obnove nakon jakog stresa. Efikasan mehanizam obnove populacije
nakon snažnog oksidativnog stresa je okarakterisan u jednoćelijskoj
bazidiomiceti, Ustilago maydis i zasniva se na proliferaciji preživelih ćelija
na račun biomolekula oslobođenih iz oštećenih i umrlih ćelija.1 Fenomen je
nazvan RUS (od engl. Repopulation under starvation, RUS) i do sada je
identifikovano 10 ćelijskih faktora uključenih u ovaj proces. Ova gljiva se
karakteriše i visokim stepenom otpornosti na UV i jonizujuće zračenje u čijoj
osnovi leži efikasna popravka DNK oštećenja. Interesantno je da su neki od
otkrivenih RUS faktora uključeni i u proces zaštite integriteta genoma.2 U
cilju otkrivanja novih faktora čija je funkcija vezana i za obnovu populacije i za
stabilnost genoma, primenjena su dva metodološka pristupa: pretraživanje
mutanata i analiza transkriptoma. Ovim pristupima je identifikovano 15
kandidata, a preliminarna analiza je potvrdila da su ovi ćelijski faktori
neophodni za oba procesa. Za dalji rad su selektovani kandidati sa
najizraženijim fenotipom i kroz molekularno-genetičke i biohemijske analize
će biti detaljnije okarakterisane njihove ćelijske funkcije., Променљивост животне средине је константна, те су жива бића морала развити
различите стратегије одговора на неповољне срединске услове. Ове стратегије се
могу сврстати у две главне групе: а) механизми резистенције/толеранције и б)
механизми обнове након јаког стреса. Ефикасан механизам обнове популације
након снажног оксидативног стреса је окарактерисан у једноћелијској
базидиомицети, Ustilago maydis и заснива се на пролиферацији преживелих ћелија
на рачун биомолекула ослобођених из оштећених и умрлих ћелија.1 Феномен је
назван RUS (од енгл. Repopulation under starvation, RUS) и до сада је
идентификовано 10 ћелијских фактора укључених у овај процес. Ова гљива се
карактерише и високим степеном отпорности на УВ и јонизујуће зрачење у чијој
основи лежи ефикасна поправка ДНК оштећења. Интересантно је да су неки од
откривених РУС фактора укључени и у процес заштите интегритета генома.2 У
циљу откривања нових фактора чија је функција везана и за обнову популације и за
стабилност генома, примењена су два методолошка приступа: претраживање
мутаната и анализа транскриптома. Овим приступима је идентификовано 15
кандидата, а прелиминарна анализа је потврдила да су ови ћелијски фактори
неопходни за оба процеса. За даљи рад су селектовани кандидати са
најизраженијим фенотипом и кроз молекуларно-генетичке и биохемијске анализе
ће бити детаљније окарактерисане њихове ћелијске функције.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Treći kongres biologa Srbije",
title = "Obnova ćelijske populacije Ustilago maydis nakon stresa i veza sa zaštitom genoma – novi ćelijski faktori, Обнова ћелијске популације Ustilago maydis након стреса и веза са заштитом генома – нови ћелијски фактори",
pages = "272",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1749"
}

Stanovčić, S., Malešević, J., Milisavljević, M.,& Kojić, M.. (2022). Obnova ćelijske populacije Ustilago maydis nakon stresa i veza sa zaštitom genoma – novi ćelijski faktori. in Treći kongres biologa Srbije
Beograd : Srpsko biološko društvo., 272.
https://hdl.handle.net/21.15107/rcub_imagine_1749

Stanovčić S, Malešević J, Milisavljević M, Kojić M. Obnova ćelijske populacije Ustilago maydis nakon stresa i veza sa zaštitom genoma – novi ćelijski faktori. in Treći kongres biologa Srbije. 2022;:272.
https://hdl.handle.net/21.15107/rcub_imagine_1749 .

Stanovčić, Stefan, Malešević, Jelena, Milisavljević, Mira, Kojić, Milorad, "Obnova ćelijske populacije Ustilago maydis nakon stresa i veza sa zaštitom genoma – novi ćelijski faktori" in Treći kongres biologa Srbije (2022):272,
https://hdl.handle.net/21.15107/rcub_imagine_1749 .

TY  - JOUR
AU  - Malešević, Jelena
AU  - Kojić, Milorad
AU  - Stanovčić, Stefan
AU  - Azanjac, Natalija
AU  - Milisavljević, Mira
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1518
AB  - Much headway has been made in understanding the numerous strategies that enable microorganisms to counteract various types of environmental stress, but little is known about how microbial populations recover after a massive death caused by exposure to extreme conditions. Using the yeast-like fungus Ustilago maydis as a model, our recent post-stress regrowth under starvation (RUS) studies have demonstrated that this organism reconstitutes devastated populations with remarkable efficiency. Subsequently, we have identified four RUS-gene products. Two of these, Did4 and Tbp1, play parallel roles in protecting the genome. To identify additional molecular components, we took a molecular-genetic and a transcriptomic approach. By employing a simple and novel screening method, we identified five RUS-deficient mutants (snf8, slm1, vrg4, snf5, hsf1), three of which (snf8, slm1, and hsf1) displayed sensitivity to different genotoxic agents, indicating that the corresponding gene products have roles in genome protection. The global transcriptomic changes of cells grown in supernatants derived from peroxide-treated cell suspensions revealed sets of uniquely expressed genes. Importantly, among the genes induced by the substrates was Chk1, which encodes a protein kinase required for checkpoint-mediated cell cycle arrest in response to DNA damage. Mutants of U. maydis deleted of Chk1 are severely incapacitated in RUS.
PB  - MDPI, Basel
T2  - Journal of Fungi
T1  - Identification of Genes Promoting Growth of Ustilago maydis on Biomolecules Released from Cells Killed by Oxidation
IS  - 9
VL  - 8
DO  - 10.3390/jof8090957
ER  - 

@article{
author = "Malešević, Jelena and Kojić, Milorad and Stanovčić, Stefan and Azanjac, Natalija and Milisavljević, Mira",
year = "2022",
abstract = "Much headway has been made in understanding the numerous strategies that enable microorganisms to counteract various types of environmental stress, but little is known about how microbial populations recover after a massive death caused by exposure to extreme conditions. Using the yeast-like fungus Ustilago maydis as a model, our recent post-stress regrowth under starvation (RUS) studies have demonstrated that this organism reconstitutes devastated populations with remarkable efficiency. Subsequently, we have identified four RUS-gene products. Two of these, Did4 and Tbp1, play parallel roles in protecting the genome. To identify additional molecular components, we took a molecular-genetic and a transcriptomic approach. By employing a simple and novel screening method, we identified five RUS-deficient mutants (snf8, slm1, vrg4, snf5, hsf1), three of which (snf8, slm1, and hsf1) displayed sensitivity to different genotoxic agents, indicating that the corresponding gene products have roles in genome protection. The global transcriptomic changes of cells grown in supernatants derived from peroxide-treated cell suspensions revealed sets of uniquely expressed genes. Importantly, among the genes induced by the substrates was Chk1, which encodes a protein kinase required for checkpoint-mediated cell cycle arrest in response to DNA damage. Mutants of U. maydis deleted of Chk1 are severely incapacitated in RUS.",
publisher = "MDPI, Basel",
journal = "Journal of Fungi",
title = "Identification of Genes Promoting Growth of Ustilago maydis on Biomolecules Released from Cells Killed by Oxidation",
number = "9",
volume = "8",
doi = "10.3390/jof8090957"
}

Malešević, J., Kojić, M., Stanovčić, S., Azanjac, N.,& Milisavljević, M.. (2022). Identification of Genes Promoting Growth of Ustilago maydis on Biomolecules Released from Cells Killed by Oxidation. in Journal of Fungi
MDPI, Basel., 8(9).
https://doi.org/10.3390/jof8090957

Malešević J, Kojić M, Stanovčić S, Azanjac N, Milisavljević M. Identification of Genes Promoting Growth of Ustilago maydis on Biomolecules Released from Cells Killed by Oxidation. in Journal of Fungi. 2022;8(9).
doi:10.3390/jof8090957 .

Malešević, Jelena, Kojić, Milorad, Stanovčić, Stefan, Azanjac, Natalija, Milisavljević, Mira, "Identification of Genes Promoting Growth of Ustilago maydis on Biomolecules Released from Cells Killed by Oxidation" in Journal of Fungi, 8, no. 9 (2022),
https://doi.org/10.3390/jof8090957 . .

TY  - JOUR
AU  - Malešević, Jelena
AU  - Kojić, Milorad
AU  - Milisavljević, Mira
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1495
AB  - Ustilago maydis and Saccharomyces cerevisiae differ considerably in their response to water-transfer treatments. When stationary phase cells were transferred to pure water and incubated under limited supply of oxygen, the U. maydis cells suffered a catastrophic loss of viability while the S. cerevisiae population was virtually unaffected by the treatment. The major factor underlying the death of the U. maydis cells under those conditions was an oxygen-consuming cellular activity that generated a hypoxic environment, thereby inducing oxidative stress and accumulation of reactive oxygen species, which resulted in lethality. Importantly, a small residue of U. maydis cells that did survive was able to resume growth and repopulate up to the initial culture density when sufficient aeration was restored. The regrowth was dependent on the cellular factors (Adr1, Did4, Kel1, and Tbp1), previously identified as required for repopulation, after killing with hydrogen peroxide. Surprisingly, the survivors were also able to resume growth under apparently hypoxic conditions, indicating that these remnant cells likely switched to a fermentative mode of growth. We discuss the findings in terms of their possible relevance to the eco-evolutionary adaptation of U. maydis to risky environments.
PB  - MDPI, Basel
T2  - Journal of Fungi
T1  - Self-Generated Hypoxia Leads to Oxidative Stress and Massive Death in Ustilago maydis Populations under Extreme Starvation and Oxygen-Limited Conditions
IS  - 2
VL  - 7
DO  - 10.3390/jof7020092
ER  - 

@article{
author = "Malešević, Jelena and Kojić, Milorad and Milisavljević, Mira",
year = "2021",
abstract = "Ustilago maydis and Saccharomyces cerevisiae differ considerably in their response to water-transfer treatments. When stationary phase cells were transferred to pure water and incubated under limited supply of oxygen, the U. maydis cells suffered a catastrophic loss of viability while the S. cerevisiae population was virtually unaffected by the treatment. The major factor underlying the death of the U. maydis cells under those conditions was an oxygen-consuming cellular activity that generated a hypoxic environment, thereby inducing oxidative stress and accumulation of reactive oxygen species, which resulted in lethality. Importantly, a small residue of U. maydis cells that did survive was able to resume growth and repopulate up to the initial culture density when sufficient aeration was restored. The regrowth was dependent on the cellular factors (Adr1, Did4, Kel1, and Tbp1), previously identified as required for repopulation, after killing with hydrogen peroxide. Surprisingly, the survivors were also able to resume growth under apparently hypoxic conditions, indicating that these remnant cells likely switched to a fermentative mode of growth. We discuss the findings in terms of their possible relevance to the eco-evolutionary adaptation of U. maydis to risky environments.",
publisher = "MDPI, Basel",
journal = "Journal of Fungi",
title = "Self-Generated Hypoxia Leads to Oxidative Stress and Massive Death in Ustilago maydis Populations under Extreme Starvation and Oxygen-Limited Conditions",
number = "2",
volume = "7",
doi = "10.3390/jof7020092"
}

Malešević, J., Kojić, M.,& Milisavljević, M.. (2021). Self-Generated Hypoxia Leads to Oxidative Stress and Massive Death in Ustilago maydis Populations under Extreme Starvation and Oxygen-Limited Conditions. in Journal of Fungi
MDPI, Basel., 7(2).
https://doi.org/10.3390/jof7020092

Malešević J, Kojić M, Milisavljević M. Self-Generated Hypoxia Leads to Oxidative Stress and Massive Death in Ustilago maydis Populations under Extreme Starvation and Oxygen-Limited Conditions. in Journal of Fungi. 2021;7(2).
doi:10.3390/jof7020092 .

Malešević, Jelena, Kojić, Milorad, Milisavljević, Mira, "Self-Generated Hypoxia Leads to Oxidative Stress and Massive Death in Ustilago maydis Populations under Extreme Starvation and Oxygen-Limited Conditions" in Journal of Fungi, 7, no. 2 (2021),
https://doi.org/10.3390/jof7020092 . .

TY  - JOUR
AU  - Kojić, Milorad
AU  - Milisavljević, Mira
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1340
AB  - Microorganisms have an assortment of stress-response mechanisms that enable them to survive in the face of environmental stresses. However, with prolonged exposures to severe stresses adaptive stress responses ultimately fail, the affected populations may suffer a massive decline. Recovery of the population density in the aftermath of a massive death is a vital task. Our recent post-stress regrowth under starvation (RUS) studies prompted us to propose RUS as an adaptation for overcoming consequences of devastating environmental disturbances. RUS should be seen as an integral process having two major aspects: the stress-induced cellular auto-decomposition and the recycling of the released nutrients. Here, we summarized what is already known about RUS and suggest a number of questions that are key to understanding the molecular underpinnings of these two operations. We also interrogate the prospect that would conceptualize the auto-decomposition as a fitness-maximizing mechanism acting with the purpose of an expedient supply of nutrients. Two further things are of special note: given that some of the RUS-defective mutants are also impaired in DNA repair, RUS can serve as an important tool for uncovering new determinants operating, in some overlapping fashion, in the protection of genome integrity; also, RUS can serve as a new angle of approach that might, hopefully, assign roles to some of those (up to similar to 30%) of microbial genes that are of unknown function. More generally, understanding post-stress reconstitution and the underlying mechanisms is a necessary (complementing) part of any comprehensive picture of how microbes cope with very harsh environmental disturbances.
PB  - Wiley, Hoboken
T2  - Molecular Ecology
T1  - When disaster strikes: Reconstitution of population density by expansion of survivors
EP  - 4764
IS  - 24
SP  - 4757
VL  - 29
DO  - 10.1111/mec.15680
ER  - 

@article{
author = "Kojić, Milorad and Milisavljević, Mira",
year = "2020",
abstract = "Microorganisms have an assortment of stress-response mechanisms that enable them to survive in the face of environmental stresses. However, with prolonged exposures to severe stresses adaptive stress responses ultimately fail, the affected populations may suffer a massive decline. Recovery of the population density in the aftermath of a massive death is a vital task. Our recent post-stress regrowth under starvation (RUS) studies prompted us to propose RUS as an adaptation for overcoming consequences of devastating environmental disturbances. RUS should be seen as an integral process having two major aspects: the stress-induced cellular auto-decomposition and the recycling of the released nutrients. Here, we summarized what is already known about RUS and suggest a number of questions that are key to understanding the molecular underpinnings of these two operations. We also interrogate the prospect that would conceptualize the auto-decomposition as a fitness-maximizing mechanism acting with the purpose of an expedient supply of nutrients. Two further things are of special note: given that some of the RUS-defective mutants are also impaired in DNA repair, RUS can serve as an important tool for uncovering new determinants operating, in some overlapping fashion, in the protection of genome integrity; also, RUS can serve as a new angle of approach that might, hopefully, assign roles to some of those (up to similar to 30%) of microbial genes that are of unknown function. More generally, understanding post-stress reconstitution and the underlying mechanisms is a necessary (complementing) part of any comprehensive picture of how microbes cope with very harsh environmental disturbances.",
publisher = "Wiley, Hoboken",
journal = "Molecular Ecology",
title = "When disaster strikes: Reconstitution of population density by expansion of survivors",
pages = "4764-4757",
number = "24",
volume = "29",
doi = "10.1111/mec.15680"
}

Kojić, M.,& Milisavljević, M.. (2020). When disaster strikes: Reconstitution of population density by expansion of survivors. in Molecular Ecology
Wiley, Hoboken., 29(24), 4757-4764.
https://doi.org/10.1111/mec.15680

Kojić M, Milisavljević M. When disaster strikes: Reconstitution of population density by expansion of survivors. in Molecular Ecology. 2020;29(24):4757-4764.
doi:10.1111/mec.15680 .

Kojić, Milorad, Milisavljević, Mira, "When disaster strikes: Reconstitution of population density by expansion of survivors" in Molecular Ecology, 29, no. 24 (2020):4757-4764,
https://doi.org/10.1111/mec.15680 . .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Kojić, Milorad
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1392
AB  - The ability of Saccharomyces cerevisiae to reconstitute viability after strong peroxide-induced oxidative stress during liquid holding (LH) in non-nutrient medium has been compared with that of Ustilago maydis. It was found that like U. maydis, S. cerevisiae was capable of reconstituting viability through multiplication of the survivors. However, differences were observed in the pattern of their response: (i) the reconstitution of viability was slower in S. cerevisiae; (ii) before the viability was progressively increasing the treated samples of this fungus reproducibly passed through a phase of additional decrease of the surviving fraction and (iii) the final yields of viable cells attained in S. cerevisiae were below those achieved by U. maydis. The reason for the relative superiority of U. maydis is twofold: (1) early initiated and faster degradation and leakage of the intracellular biomolecules and (2) greater ability of U. maydis cells to recycle damaged and released intracellular compounds. Conceptually similar studies extended to another oxidative-stress-inducing condition, namely desiccation, indicated that the marked differences between these fungi in their patterns of the post-stress regrowth, cellular leakage and macromolecule decomposition are reproduced during LH of desiccated cells. The concordance of the findings obtained upon these two approaches was also corroborated by an analysis of the post-desiccation LH response of U. maydis mutants (adri, did4, kell and tbp1) that were previously identified as defective in post-peroxide LH restitution of viability. We discuss the findings in terms of their possible relevance to the mechanisms of the ecological and evolutionary adaptation of free-living microorganisms to fluctuating and severely inhospitable environments.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Fungal Genetics and Biology
T1  - A comparative study of liquid holding restitution of viability after oxidative stress in Ustilago maydis and Saccharomyces cerevisiae cell populations
VL  - 134
DO  - 10.1016/j.fgb.2019.103284
ER  - 

@article{
author = "Milisavljević, Mira and Kojić, Milorad",
year = "2020",
abstract = "The ability of Saccharomyces cerevisiae to reconstitute viability after strong peroxide-induced oxidative stress during liquid holding (LH) in non-nutrient medium has been compared with that of Ustilago maydis. It was found that like U. maydis, S. cerevisiae was capable of reconstituting viability through multiplication of the survivors. However, differences were observed in the pattern of their response: (i) the reconstitution of viability was slower in S. cerevisiae; (ii) before the viability was progressively increasing the treated samples of this fungus reproducibly passed through a phase of additional decrease of the surviving fraction and (iii) the final yields of viable cells attained in S. cerevisiae were below those achieved by U. maydis. The reason for the relative superiority of U. maydis is twofold: (1) early initiated and faster degradation and leakage of the intracellular biomolecules and (2) greater ability of U. maydis cells to recycle damaged and released intracellular compounds. Conceptually similar studies extended to another oxidative-stress-inducing condition, namely desiccation, indicated that the marked differences between these fungi in their patterns of the post-stress regrowth, cellular leakage and macromolecule decomposition are reproduced during LH of desiccated cells. The concordance of the findings obtained upon these two approaches was also corroborated by an analysis of the post-desiccation LH response of U. maydis mutants (adri, did4, kell and tbp1) that were previously identified as defective in post-peroxide LH restitution of viability. We discuss the findings in terms of their possible relevance to the mechanisms of the ecological and evolutionary adaptation of free-living microorganisms to fluctuating and severely inhospitable environments.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Fungal Genetics and Biology",
title = "A comparative study of liquid holding restitution of viability after oxidative stress in Ustilago maydis and Saccharomyces cerevisiae cell populations",
volume = "134",
doi = "10.1016/j.fgb.2019.103284"
}

Milisavljević, M.,& Kojić, M.. (2020). A comparative study of liquid holding restitution of viability after oxidative stress in Ustilago maydis and Saccharomyces cerevisiae cell populations. in Fungal Genetics and Biology
Academic Press Inc Elsevier Science, San Diego., 134.
https://doi.org/10.1016/j.fgb.2019.103284

Milisavljević M, Kojić M. A comparative study of liquid holding restitution of viability after oxidative stress in Ustilago maydis and Saccharomyces cerevisiae cell populations. in Fungal Genetics and Biology. 2020;134.
doi:10.1016/j.fgb.2019.103284 .

Milisavljević, Mira, Kojić, Milorad, "A comparative study of liquid holding restitution of viability after oxidative stress in Ustilago maydis and Saccharomyces cerevisiae cell populations" in Fungal Genetics and Biology, 134 (2020),
https://doi.org/10.1016/j.fgb.2019.103284 . .

TY  - JOUR
AU  - Kojić, Milorad
AU  - Milisavljević, Mira
AU  - Holloman, William K.
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1190
AB  - Cells maintain a small arsenal of resolving functions to process and eliminate complex DNA intermediates that result as a consequence of homologous recombination and distressed replication. Ordinarily the homologous recombination system serves as a high-fidelity mechanism to restore the integrity of a damaged genome, but in the absence of the appropriate resolving function it can turn DNA intermediates resulting from replication stress into pathological forms that are toxic to cells. Here we have investigated how the nucleases Mus81 and Gen1 and the helicase Blm contribute to survival after DNA damage or replication stress in Ustilago maydis cells with crippled yet homologous recombination-proficient forms of Brh2, the BRCA2 ortholog and primary Rad51 mediator. We found collaboration among the factors. Notable were three findings. First, the ability of Gen1 to rescue hydroxyurea sensitivity of dysfunctional Blm requires the absence of Mus81. Second, the response of mutants defective in Blm and Gen1 to hydroxyurea challenge is markedly similar suggesting cooperation of these factors in the same pathway. Third, the repair proficiency of Brh2 mutant variants deleted of its N-terminal DNA binding region requires not only Rad52 but also Gen1. and Mus81. We suggest these factors comprise a sub pathway for channeling repair when Brh2 is compromised in its interplay with DNA.
PB  - Elsevier Science Bv, Amsterdam
T2  - DNA Repair
T1  - Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis
EP  - 55
SP  - 47
VL  - 63
DO  - 10.1016/j.dnarep.2018.01.010
ER  - 

@article{
author = "Kojić, Milorad and Milisavljević, Mira and Holloman, William K.",
year = "2018",
abstract = "Cells maintain a small arsenal of resolving functions to process and eliminate complex DNA intermediates that result as a consequence of homologous recombination and distressed replication. Ordinarily the homologous recombination system serves as a high-fidelity mechanism to restore the integrity of a damaged genome, but in the absence of the appropriate resolving function it can turn DNA intermediates resulting from replication stress into pathological forms that are toxic to cells. Here we have investigated how the nucleases Mus81 and Gen1 and the helicase Blm contribute to survival after DNA damage or replication stress in Ustilago maydis cells with crippled yet homologous recombination-proficient forms of Brh2, the BRCA2 ortholog and primary Rad51 mediator. We found collaboration among the factors. Notable were three findings. First, the ability of Gen1 to rescue hydroxyurea sensitivity of dysfunctional Blm requires the absence of Mus81. Second, the response of mutants defective in Blm and Gen1 to hydroxyurea challenge is markedly similar suggesting cooperation of these factors in the same pathway. Third, the repair proficiency of Brh2 mutant variants deleted of its N-terminal DNA binding region requires not only Rad52 but also Gen1. and Mus81. We suggest these factors comprise a sub pathway for channeling repair when Brh2 is compromised in its interplay with DNA.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "DNA Repair",
title = "Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis",
pages = "55-47",
volume = "63",
doi = "10.1016/j.dnarep.2018.01.010"
}

Kojić, M., Milisavljević, M.,& Holloman, W. K.. (2018). Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis. in DNA Repair
Elsevier Science Bv, Amsterdam., 63, 47-55.
https://doi.org/10.1016/j.dnarep.2018.01.010

Kojić M, Milisavljević M, Holloman WK. Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis. in DNA Repair. 2018;63:47-55.
doi:10.1016/j.dnarep.2018.01.010 .

Kojić, Milorad, Milisavljević, Mira, Holloman, William K., "Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis" in DNA Repair, 63 (2018):47-55,
https://doi.org/10.1016/j.dnarep.2018.01.010 . .

TY  - JOUR
AU  - Kojić, Milorad
AU  - Milisavljević, Mira
AU  - Holloman, William K.
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1626
AB  - Cells maintain a small arsenal of resolving functions to process and eliminate complex DNA intermediates that result as a consequence of homologous recombination and distressed replication. Ordinarily the homologous recombination system serves as a high-fidelity mechanism to restore the integrity of a damaged genome, but in the absence of the appropriate resolving function it can turn DNA intermediates resulting from replication stress into pathological forms that are toxic to cells. Here we have investigated how the nucleases Mus81 and Gen1 and the helicase Blm contribute to survival after DNA damage or replication stress in Ustilago maydis cells with crippled yet homologous recombination-proficient forms of Brh2, the BRCA2 ortholog and primary Rad51 mediator. We found collaboration among the factors. Notable were three findings. First, the ability of Gen1 to rescue hydroxyurea sensitivity of dysfunctional Blm requires the absence of Mus81. Second, the response of mutants defective in Blm and Gen1 to hydroxyurea challenge is markedly similar suggesting cooperation of these factors in the same pathway. Third, the repair proficiency of Brh2 mutant variants deleted of its N-terminal DNA binding region requires not only Rad52 but also Gen1. and Mus81. We suggest these factors comprise a sub pathway for channeling repair when Brh2 is compromised in its interplay with DNA.
PB  - Elsevier Science Bv, Amsterdam
T2  - DNA Repair
T1  - Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis
EP  - 55
SP  - 47
VL  - 63
DO  - 10.1016/j.dnarep.2018.01.010
ER  - 

@article{
author = "Kojić, Milorad and Milisavljević, Mira and Holloman, William K.",
year = "2018",
abstract = "Cells maintain a small arsenal of resolving functions to process and eliminate complex DNA intermediates that result as a consequence of homologous recombination and distressed replication. Ordinarily the homologous recombination system serves as a high-fidelity mechanism to restore the integrity of a damaged genome, but in the absence of the appropriate resolving function it can turn DNA intermediates resulting from replication stress into pathological forms that are toxic to cells. Here we have investigated how the nucleases Mus81 and Gen1 and the helicase Blm contribute to survival after DNA damage or replication stress in Ustilago maydis cells with crippled yet homologous recombination-proficient forms of Brh2, the BRCA2 ortholog and primary Rad51 mediator. We found collaboration among the factors. Notable were three findings. First, the ability of Gen1 to rescue hydroxyurea sensitivity of dysfunctional Blm requires the absence of Mus81. Second, the response of mutants defective in Blm and Gen1 to hydroxyurea challenge is markedly similar suggesting cooperation of these factors in the same pathway. Third, the repair proficiency of Brh2 mutant variants deleted of its N-terminal DNA binding region requires not only Rad52 but also Gen1. and Mus81. We suggest these factors comprise a sub pathway for channeling repair when Brh2 is compromised in its interplay with DNA.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "DNA Repair",
title = "Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis",
pages = "55-47",
volume = "63",
doi = "10.1016/j.dnarep.2018.01.010"
}

Kojić, M., Milisavljević, M.,& Holloman, W. K.. (2018). Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis. in DNA Repair
Elsevier Science Bv, Amsterdam., 63, 47-55.
https://doi.org/10.1016/j.dnarep.2018.01.010

Kojić M, Milisavljević M, Holloman WK. Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis. in DNA Repair. 2018;63:47-55.
doi:10.1016/j.dnarep.2018.01.010 .

Kojić, Milorad, Milisavljević, Mira, Holloman, William K., "Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis" in DNA Repair, 63 (2018):47-55,
https://doi.org/10.1016/j.dnarep.2018.01.010 . .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Petković, Jelena
AU  - Samardžić, Jelena
AU  - Kojić, Milorad
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1186
AB  - After heavy exposure of Ustilago maydis cells to clastogens, a great increase in viability was observed if the treated cells were kept under starvation conditions This restitution of viability is based on cell multiplication at the expense of the intracellular compounds freed from the damaged cells. Analysis of the effect of the leaked material on the growth of undamaged cells revealed opposing biological activity, indicating that U. maydis must possess cellular mechanisms involved not only in reabsorption of the released compounds from external environment but also in contending with their treatment-induced toxicity. From a screen for mutants defective in the restitution of viability, we identified four genes (adr1, did4, kel1, and tbp1) that contribute to the process The mutants in did4, kel1, and tbp1 exhibited sensitivity to different genotoxic agents implying that the gene products are in some overlapping fashion involved in the protection of genome integrity The genetic determinants identified by our analysis have already been known to play roles in growth regulation, protein turnover, cytoskeleton structure, and transcription. We discuss ecological and evolutionary implications of these results.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations
VL  - 9
DO  - 10.3389/fmicb.2018.00990
ER  - 

@article{
author = "Milisavljević, Mira and Petković, Jelena and Samardžić, Jelena and Kojić, Milorad",
year = "2018",
abstract = "After heavy exposure of Ustilago maydis cells to clastogens, a great increase in viability was observed if the treated cells were kept under starvation conditions This restitution of viability is based on cell multiplication at the expense of the intracellular compounds freed from the damaged cells. Analysis of the effect of the leaked material on the growth of undamaged cells revealed opposing biological activity, indicating that U. maydis must possess cellular mechanisms involved not only in reabsorption of the released compounds from external environment but also in contending with their treatment-induced toxicity. From a screen for mutants defective in the restitution of viability, we identified four genes (adr1, did4, kel1, and tbp1) that contribute to the process The mutants in did4, kel1, and tbp1 exhibited sensitivity to different genotoxic agents implying that the gene products are in some overlapping fashion involved in the protection of genome integrity The genetic determinants identified by our analysis have already been known to play roles in growth regulation, protein turnover, cytoskeleton structure, and transcription. We discuss ecological and evolutionary implications of these results.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations",
volume = "9",
doi = "10.3389/fmicb.2018.00990"
}

Milisavljević, M., Petković, J., Samardžić, J.,& Kojić, M.. (2018). Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fmicb.2018.00990

Milisavljević M, Petković J, Samardžić J, Kojić M. Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations. in Frontiers in Microbiology. 2018;9.
doi:10.3389/fmicb.2018.00990 .

Milisavljević, Mira, Petković, Jelena, Samardžić, Jelena, Kojić, Milorad, "Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations" in Frontiers in Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.00990 . .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Zivković, S.
AU  - Pekmezović, M.
AU  - Stanković, Nada
AU  - Vojnović, Sandra
AU  - Vasiljević, Branka
AU  - Šenerović, Lidija
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/876
AB  - AimsThe aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range of fungal pathogens and its potential to control plant fungal diseases. Methods and ResultsThe antifungal activity of DDHR invitro was examined against common human and plant pathogenic fungi using a broth microdilution assay and a disk diffusion assay. Minimum inhibitory concentrations ranged from 125 to 35gml(-1). A radial growth inhibition assay showed that DDHR inhibited mycelia growth, inducing mycelial necrosis and affecting sporulation. During the invivo assay on apple fruits administration of DDHR 1h before fungal inoculation inhibited spreading of the infection. Importantly, DDHR exhibited no phytotoxic effects on the model plant, Capsicum annum, verified by the plant growth rate and chlorophyll content. ConclusionsDDHR inhibits growth of various plant pathogens invitro with the strongest activity against Alternaria alternata, Colletotrichum acutatum and Penicillium expansum, and protects apple fruits from decay. Significance and Impact of the StudyThis is the first report of the inhibitory effect of DDHR on important pathogenic fungal isolates. DDHR could be a good scaffold for developing new antifungal agents for fruit and vegetable protection.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin
EP  - 1434
IS  - 6
SP  - 1426
VL  - 118
DO  - 10.1111/jam.12811
ER  - 

@article{
author = "Milisavljević, Mira and Zivković, S. and Pekmezović, M. and Stanković, Nada and Vojnović, Sandra and Vasiljević, Branka and Šenerović, Lidija",
year = "2015",
abstract = "AimsThe aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range of fungal pathogens and its potential to control plant fungal diseases. Methods and ResultsThe antifungal activity of DDHR invitro was examined against common human and plant pathogenic fungi using a broth microdilution assay and a disk diffusion assay. Minimum inhibitory concentrations ranged from 125 to 35gml(-1). A radial growth inhibition assay showed that DDHR inhibited mycelia growth, inducing mycelial necrosis and affecting sporulation. During the invivo assay on apple fruits administration of DDHR 1h before fungal inoculation inhibited spreading of the infection. Importantly, DDHR exhibited no phytotoxic effects on the model plant, Capsicum annum, verified by the plant growth rate and chlorophyll content. ConclusionsDDHR inhibits growth of various plant pathogens invitro with the strongest activity against Alternaria alternata, Colletotrichum acutatum and Penicillium expansum, and protects apple fruits from decay. Significance and Impact of the StudyThis is the first report of the inhibitory effect of DDHR on important pathogenic fungal isolates. DDHR could be a good scaffold for developing new antifungal agents for fruit and vegetable protection.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin",
pages = "1434-1426",
number = "6",
volume = "118",
doi = "10.1111/jam.12811"
}

Milisavljević, M., Zivković, S., Pekmezović, M., Stanković, N., Vojnović, S., Vasiljević, B.,& Šenerović, L.. (2015). Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. in Journal of Applied Microbiology
Wiley, Hoboken., 118(6), 1426-1434.
https://doi.org/10.1111/jam.12811

Milisavljević M, Zivković S, Pekmezović M, Stanković N, Vojnović S, Vasiljević B, Šenerović L. Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. in Journal of Applied Microbiology. 2015;118(6):1426-1434.
doi:10.1111/jam.12811 .

Milisavljević, Mira, Zivković, S., Pekmezović, M., Stanković, Nada, Vojnović, Sandra, Vasiljević, Branka, Šenerović, Lidija, "Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin" in Journal of Applied Microbiology, 118, no. 6 (2015):1426-1434,
https://doi.org/10.1111/jam.12811 . .

TY  - JOUR
AU  - Kostić, Ljiljana
AU  - Nikolić, Nina
AU  - Samardžić, Jelena
AU  - Milisavljević, Mira
AU  - Maksimović, Vuk
AU  - Cakmak, Dragan
AU  - Manojlović, Dragan
AU  - Nikolić, Miroslav
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/890
AB  - We studied the effect of liming and P fertilization of extremely acid soil (accidently acidified by sulfidic mining waste) on P availability and the subsequent adaptive responses of wheat roots. The wheat plants were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil for sequential extraction of P fractions and determination of exchangeable Al. Root exudates were collected by pieces of paper for electrophoresis and subjected to HPLC analysis. Expression of organic anions and P-i transporter genes was analyzed by a real-time quantitative PCR. The concomitant application of lime with P fertilization increased the concentrations of plant-available P fractions in both rhizosphere and bulk compartments. The applied soil amendments strongly affected plant growth, biomass partitioning and shoot P accumulation. Liming enhanced root exudation of citrate in P unfertilized plants, while the high malate efflux was maintained until both P deficiency and Al toxicity were eliminated by the amendments. We showed the importance of liming for recovering of P acquisition potential of wheat roots, which can be strongly impaired in acid soils. Our results clearly demonstrated that P-deficient roots not subjected to Al stress in the limed soil can maintain high efflux of malate and even increase efflux of citrate along with the enhanced expression of related anion transporters (TaMATE1 and TaALMT1).
PB  - Springer, New York
T2  - Biology and Fertility of Soils
T1  - Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat
EP  - 298
IS  - 3
SP  - 289
VL  - 51
DO  - 10.1007/s00374-014-0975-y
ER  - 

@article{
author = "Kostić, Ljiljana and Nikolić, Nina and Samardžić, Jelena and Milisavljević, Mira and Maksimović, Vuk and Cakmak, Dragan and Manojlović, Dragan and Nikolić, Miroslav",
year = "2015",
abstract = "We studied the effect of liming and P fertilization of extremely acid soil (accidently acidified by sulfidic mining waste) on P availability and the subsequent adaptive responses of wheat roots. The wheat plants were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil for sequential extraction of P fractions and determination of exchangeable Al. Root exudates were collected by pieces of paper for electrophoresis and subjected to HPLC analysis. Expression of organic anions and P-i transporter genes was analyzed by a real-time quantitative PCR. The concomitant application of lime with P fertilization increased the concentrations of plant-available P fractions in both rhizosphere and bulk compartments. The applied soil amendments strongly affected plant growth, biomass partitioning and shoot P accumulation. Liming enhanced root exudation of citrate in P unfertilized plants, while the high malate efflux was maintained until both P deficiency and Al toxicity were eliminated by the amendments. We showed the importance of liming for recovering of P acquisition potential of wheat roots, which can be strongly impaired in acid soils. Our results clearly demonstrated that P-deficient roots not subjected to Al stress in the limed soil can maintain high efflux of malate and even increase efflux of citrate along with the enhanced expression of related anion transporters (TaMATE1 and TaALMT1).",
publisher = "Springer, New York",
journal = "Biology and Fertility of Soils",
title = "Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat",
pages = "298-289",
number = "3",
volume = "51",
doi = "10.1007/s00374-014-0975-y"
}

Kostić, L., Nikolić, N., Samardžić, J., Milisavljević, M., Maksimović, V., Cakmak, D., Manojlović, D.,& Nikolić, M.. (2015). Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat. in Biology and Fertility of Soils
Springer, New York., 51(3), 289-298.
https://doi.org/10.1007/s00374-014-0975-y

Kostić L, Nikolić N, Samardžić J, Milisavljević M, Maksimović V, Cakmak D, Manojlović D, Nikolić M. Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat. in Biology and Fertility of Soils. 2015;51(3):289-298.
doi:10.1007/s00374-014-0975-y .

Kostić, Ljiljana, Nikolić, Nina, Samardžić, Jelena, Milisavljević, Mira, Maksimović, Vuk, Cakmak, Dragan, Manojlović, Dragan, Nikolić, Miroslav, "Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat" in Biology and Fertility of Soils, 51, no. 3 (2015):289-298,
https://doi.org/10.1007/s00374-014-0975-y . .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Zivković, S.
AU  - Pekmezović, M.
AU  - Stanković, Nada
AU  - Vojnović, Sandra
AU  - Vasiljević, Branka
AU  - Šenerović, Lidija
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1608
AB  - AimsThe aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range of fungal pathogens and its potential to control plant fungal diseases. Methods and ResultsThe antifungal activity of DDHR invitro was examined against common human and plant pathogenic fungi using a broth microdilution assay and a disk diffusion assay. Minimum inhibitory concentrations ranged from 125 to 35gml(-1). A radial growth inhibition assay showed that DDHR inhibited mycelia growth, inducing mycelial necrosis and affecting sporulation. During the invivo assay on apple fruits administration of DDHR 1h before fungal inoculation inhibited spreading of the infection. Importantly, DDHR exhibited no phytotoxic effects on the model plant, Capsicum annum, verified by the plant growth rate and chlorophyll content. ConclusionsDDHR inhibits growth of various plant pathogens invitro with the strongest activity against Alternaria alternata, Colletotrichum acutatum and Penicillium expansum, and protects apple fruits from decay. Significance and Impact of the StudyThis is the first report of the inhibitory effect of DDHR on important pathogenic fungal isolates. DDHR could be a good scaffold for developing new antifungal agents for fruit and vegetable protection.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin
EP  - 1434
IS  - 6
SP  - 1426
VL  - 118
DO  - 10.1111/jam.12811
ER  - 

@article{
author = "Milisavljević, Mira and Zivković, S. and Pekmezović, M. and Stanković, Nada and Vojnović, Sandra and Vasiljević, Branka and Šenerović, Lidija",
year = "2015",
abstract = "AimsThe aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range of fungal pathogens and its potential to control plant fungal diseases. Methods and ResultsThe antifungal activity of DDHR invitro was examined against common human and plant pathogenic fungi using a broth microdilution assay and a disk diffusion assay. Minimum inhibitory concentrations ranged from 125 to 35gml(-1). A radial growth inhibition assay showed that DDHR inhibited mycelia growth, inducing mycelial necrosis and affecting sporulation. During the invivo assay on apple fruits administration of DDHR 1h before fungal inoculation inhibited spreading of the infection. Importantly, DDHR exhibited no phytotoxic effects on the model plant, Capsicum annum, verified by the plant growth rate and chlorophyll content. ConclusionsDDHR inhibits growth of various plant pathogens invitro with the strongest activity against Alternaria alternata, Colletotrichum acutatum and Penicillium expansum, and protects apple fruits from decay. Significance and Impact of the StudyThis is the first report of the inhibitory effect of DDHR on important pathogenic fungal isolates. DDHR could be a good scaffold for developing new antifungal agents for fruit and vegetable protection.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin",
pages = "1434-1426",
number = "6",
volume = "118",
doi = "10.1111/jam.12811"
}

Milisavljević, M., Zivković, S., Pekmezović, M., Stanković, N., Vojnović, S., Vasiljević, B.,& Šenerović, L.. (2015). Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. in Journal of Applied Microbiology
Wiley, Hoboken., 118(6), 1426-1434.
https://doi.org/10.1111/jam.12811

Milisavljević M, Zivković S, Pekmezović M, Stanković N, Vojnović S, Vasiljević B, Šenerović L. Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. in Journal of Applied Microbiology. 2015;118(6):1426-1434.
doi:10.1111/jam.12811 .

Milisavljević, Mira, Zivković, S., Pekmezović, M., Stanković, Nada, Vojnović, Sandra, Vasiljević, Branka, Šenerović, Lidija, "Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin" in Journal of Applied Microbiology, 118, no. 6 (2015):1426-1434,
https://doi.org/10.1111/jam.12811 . .

TY  - JOUR
AU  - de Sena-Tomas, Carmen
AU  - Sutherland, Jeanette H.
AU  - Milisavljević, Mira
AU  - Nikolić, Dragana
AU  - Perez-Martin, Jose
AU  - Kojić, Milorad
AU  - Holloman, William K.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1624
AB  - Here we report identification of the lkh1 gene encoding a LAMMER kinase homolog (Lkh1) from a screen for DNA repair-deficient mutants in Ustilago maydis. The mutant allele isolated results from a mutation at glutamine codon 488 to a stop codon that would be predicted to lead to truncation of the carboxyterminal kinase domain of the protein. This mutant (lkh1(Q488*)) is highly sensitive to ultraviolet light, methyl methanesulfonate, and hydroxyurea. In contrast, a null mutant (lkh1 Delta) deleted of the entire lkh1 gene has a less severe phenotype. No epistasis was observed when an lkh1(Q488*) rad51 Delta double mutant was tested for genotoxin sensitivity. However, overexpressing the gene for Rad51, its regulator Brh2, or the Brh2 regulator Dss1 partially restored genotoxin resistance of the lkh1 Delta and lkh1(Q488*) mutants. Deletion of lkh1 in a chk1 Delta mutant enabled these double mutant cells to continue to cycle when challenged with hydroxyurea. lkh1 Delta and lkh1(Q488*) mutants were able to complete the meiotic process but exhibited reduced heteroallelic recombination and aberrant chromosome segregation. The observations suggest that Lkh1 serves in some aspect of cell cycle regulation after DNA damage or replication stress and that it also contributes to proper chromosome segregation in meiosis.
PB  - Amsterdam : Elsevier
T2  - DNA Repair
T1  - LAMMER kinase contributes to genome stability in Ustilago maydis
EP  - 77
SP  - 70
VL  - 33
DO  - 10.1016/j.dnarep.2015.05.011
ER  - 

@article{
author = "de Sena-Tomas, Carmen and Sutherland, Jeanette H. and Milisavljević, Mira and Nikolić, Dragana and Perez-Martin, Jose and Kojić, Milorad and Holloman, William K.",
year = "2015",
abstract = "Here we report identification of the lkh1 gene encoding a LAMMER kinase homolog (Lkh1) from a screen for DNA repair-deficient mutants in Ustilago maydis. The mutant allele isolated results from a mutation at glutamine codon 488 to a stop codon that would be predicted to lead to truncation of the carboxyterminal kinase domain of the protein. This mutant (lkh1(Q488*)) is highly sensitive to ultraviolet light, methyl methanesulfonate, and hydroxyurea. In contrast, a null mutant (lkh1 Delta) deleted of the entire lkh1 gene has a less severe phenotype. No epistasis was observed when an lkh1(Q488*) rad51 Delta double mutant was tested for genotoxin sensitivity. However, overexpressing the gene for Rad51, its regulator Brh2, or the Brh2 regulator Dss1 partially restored genotoxin resistance of the lkh1 Delta and lkh1(Q488*) mutants. Deletion of lkh1 in a chk1 Delta mutant enabled these double mutant cells to continue to cycle when challenged with hydroxyurea. lkh1 Delta and lkh1(Q488*) mutants were able to complete the meiotic process but exhibited reduced heteroallelic recombination and aberrant chromosome segregation. The observations suggest that Lkh1 serves in some aspect of cell cycle regulation after DNA damage or replication stress and that it also contributes to proper chromosome segregation in meiosis.",
publisher = "Amsterdam : Elsevier",
journal = "DNA Repair",
title = "LAMMER kinase contributes to genome stability in Ustilago maydis",
pages = "77-70",
volume = "33",
doi = "10.1016/j.dnarep.2015.05.011"
}

de Sena-Tomas, C., Sutherland, J. H., Milisavljević, M., Nikolić, D., Perez-Martin, J., Kojić, M.,& Holloman, W. K.. (2015). LAMMER kinase contributes to genome stability in Ustilago maydis. in DNA Repair
Amsterdam : Elsevier., 33, 70-77.
https://doi.org/10.1016/j.dnarep.2015.05.011

de Sena-Tomas C, Sutherland JH, Milisavljević M, Nikolić D, Perez-Martin J, Kojić M, Holloman WK. LAMMER kinase contributes to genome stability in Ustilago maydis. in DNA Repair. 2015;33:70-77.
doi:10.1016/j.dnarep.2015.05.011 .

de Sena-Tomas, Carmen, Sutherland, Jeanette H., Milisavljević, Mira, Nikolić, Dragana, Perez-Martin, Jose, Kojić, Milorad, Holloman, William K., "LAMMER kinase contributes to genome stability in Ustilago maydis" in DNA Repair, 33 (2015):70-77,
https://doi.org/10.1016/j.dnarep.2015.05.011 . .

TY  - JOUR
AU  - Timotijević, Gordana
AU  - Milisavljević, Mira
AU  - Nikolić, Dragana
AU  - Milovanović, Bosko M.
AU  - Nikolić, Dragana
AU  - Nikolić, Miroslav
AU  - Samardžić, Jelena
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/843
AB  - MicroRNAs (miRNAs) belong to the class of small non-coding RNAs which have important roles throughout development as well as in plant response to diverse environmental stresses. Some of plant miRNAs are essential for regulation and maintenance of nutritive homeostasis when nutrients are in excess or shortage comparing to optimal concentration for certain plant species. Better understanding of miRNAs functions implies development of efficient technology for profiling their gene expression. We set out to establish validate the methodology for miRNA gene expression analysis in cucumber grown under suboptimal mineral nutrient regimes, including iron deficiency. Reverse transcription by "stem-loop" primers in combination with Real time PCR method is one of potential approaches for quantification of miRNA gene expression. In this paper we presented a method for "stem loop" primer design specific for miR398, as well as reaction optimization and determination of Real time PCR efficiency. Proving the accuracy of this method was imperative as "stem loop" RT which consider separate transcription of target and endogenous control. The method was verified by comparison of the obtained data with results of miR398 expression achieved using a commercial kit based on simultaneous conversion of all RNAs in cDNAs.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis
EP  - 416
IS  - 2
SP  - 405
VL  - 47
DO  - 10.2298/GENSR1502405T
ER  - 

@article{
author = "Timotijević, Gordana and Milisavljević, Mira and Nikolić, Dragana and Milovanović, Bosko M. and Nikolić, Dragana and Nikolić, Miroslav and Samardžić, Jelena",
year = "2015",
abstract = "MicroRNAs (miRNAs) belong to the class of small non-coding RNAs which have important roles throughout development as well as in plant response to diverse environmental stresses. Some of plant miRNAs are essential for regulation and maintenance of nutritive homeostasis when nutrients are in excess or shortage comparing to optimal concentration for certain plant species. Better understanding of miRNAs functions implies development of efficient technology for profiling their gene expression. We set out to establish validate the methodology for miRNA gene expression analysis in cucumber grown under suboptimal mineral nutrient regimes, including iron deficiency. Reverse transcription by "stem-loop" primers in combination with Real time PCR method is one of potential approaches for quantification of miRNA gene expression. In this paper we presented a method for "stem loop" primer design specific for miR398, as well as reaction optimization and determination of Real time PCR efficiency. Proving the accuracy of this method was imperative as "stem loop" RT which consider separate transcription of target and endogenous control. The method was verified by comparison of the obtained data with results of miR398 expression achieved using a commercial kit based on simultaneous conversion of all RNAs in cDNAs.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis",
pages = "416-405",
number = "2",
volume = "47",
doi = "10.2298/GENSR1502405T"
}

Timotijević, G., Milisavljević, M., Nikolić, D., Milovanović, B. M., Nikolić, D., Nikolić, M.,& Samardžić, J.. (2015). Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 47(2), 405-416.
https://doi.org/10.2298/GENSR1502405T

Timotijević G, Milisavljević M, Nikolić D, Milovanović BM, Nikolić D, Nikolić M, Samardžić J. Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis. in Genetika-Belgrade. 2015;47(2):405-416.
doi:10.2298/GENSR1502405T .

Timotijević, Gordana, Milisavljević, Mira, Nikolić, Dragana, Milovanović, Bosko M., Nikolić, Dragana, Nikolić, Miroslav, Samardžić, Jelena, "Establishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysis" in Genetika-Belgrade, 47, no. 2 (2015):405-416,
https://doi.org/10.2298/GENSR1502405T . .

TY  - JOUR
AU  - de Sena-Tomas, Carmen
AU  - Sutherland, Jeanette H.
AU  - Milisavljević, Mira
AU  - Nikolić, Dragana
AU  - Perez-Martin, Jose
AU  - Kojić, Milorad
AU  - Holloman, William K.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/887
AB  - Here we report identification of the lkh1 gene encoding a LAMMER kinase homolog (Lkh1) from a screen for DNA repair-deficient mutants in Ustilago maydis. The mutant allele isolated results from a mutation at glutamine codon 488 to a stop codon that would be predicted to lead to truncation of the carboxyterminal kinase domain of the protein. This mutant (lkh1(Q488*)) is highly sensitive to ultraviolet light, methyl methanesulfonate, and hydroxyurea. In contrast, a null mutant (lkh1 Delta) deleted of the entire lkh1 gene has a less severe phenotype. No epistasis was observed when an lkh1(Q488*) rad51 Delta double mutant was tested for genotoxin sensitivity. However, overexpressing the gene for Rad51, its regulator Brh2, or the Brh2 regulator Dss1 partially restored genotoxin resistance of the lkh1 Delta and lkh1(Q488*) mutants. Deletion of lkh1 in a chk1 Delta mutant enabled these double mutant cells to continue to cycle when challenged with hydroxyurea. lkh1 Delta and lkh1(Q488*) mutants were able to complete the meiotic process but exhibited reduced heteroallelic recombination and aberrant chromosome segregation. The observations suggest that Lkh1 serves in some aspect of cell cycle regulation after DNA damage or replication stress and that it also contributes to proper chromosome segregation in meiosis.
PB  - Amsterdam : Elsevier
T2  - DNA Repair
T1  - LAMMER kinase contributes to genome stability in Ustilago maydis
EP  - 77
SP  - 70
VL  - 33
DO  - 10.1016/j.dnarep.2015.05.011
ER  - 

@article{
author = "de Sena-Tomas, Carmen and Sutherland, Jeanette H. and Milisavljević, Mira and Nikolić, Dragana and Perez-Martin, Jose and Kojić, Milorad and Holloman, William K.",
year = "2015",
abstract = "Here we report identification of the lkh1 gene encoding a LAMMER kinase homolog (Lkh1) from a screen for DNA repair-deficient mutants in Ustilago maydis. The mutant allele isolated results from a mutation at glutamine codon 488 to a stop codon that would be predicted to lead to truncation of the carboxyterminal kinase domain of the protein. This mutant (lkh1(Q488*)) is highly sensitive to ultraviolet light, methyl methanesulfonate, and hydroxyurea. In contrast, a null mutant (lkh1 Delta) deleted of the entire lkh1 gene has a less severe phenotype. No epistasis was observed when an lkh1(Q488*) rad51 Delta double mutant was tested for genotoxin sensitivity. However, overexpressing the gene for Rad51, its regulator Brh2, or the Brh2 regulator Dss1 partially restored genotoxin resistance of the lkh1 Delta and lkh1(Q488*) mutants. Deletion of lkh1 in a chk1 Delta mutant enabled these double mutant cells to continue to cycle when challenged with hydroxyurea. lkh1 Delta and lkh1(Q488*) mutants were able to complete the meiotic process but exhibited reduced heteroallelic recombination and aberrant chromosome segregation. The observations suggest that Lkh1 serves in some aspect of cell cycle regulation after DNA damage or replication stress and that it also contributes to proper chromosome segregation in meiosis.",
publisher = "Amsterdam : Elsevier",
journal = "DNA Repair",
title = "LAMMER kinase contributes to genome stability in Ustilago maydis",
pages = "77-70",
volume = "33",
doi = "10.1016/j.dnarep.2015.05.011"
}

de Sena-Tomas, C., Sutherland, J. H., Milisavljević, M., Nikolić, D., Perez-Martin, J., Kojić, M.,& Holloman, W. K.. (2015). LAMMER kinase contributes to genome stability in Ustilago maydis. in DNA Repair
Amsterdam : Elsevier., 33, 70-77.
https://doi.org/10.1016/j.dnarep.2015.05.011

de Sena-Tomas C, Sutherland JH, Milisavljević M, Nikolić D, Perez-Martin J, Kojić M, Holloman WK. LAMMER kinase contributes to genome stability in Ustilago maydis. in DNA Repair. 2015;33:70-77.
doi:10.1016/j.dnarep.2015.05.011 .

de Sena-Tomas, Carmen, Sutherland, Jeanette H., Milisavljević, Mira, Nikolić, Dragana, Perez-Martin, Jose, Kojić, Milorad, Holloman, William K., "LAMMER kinase contributes to genome stability in Ustilago maydis" in DNA Repair, 33 (2015):70-77,
https://doi.org/10.1016/j.dnarep.2015.05.011 . .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Timotijević, Gordana
AU  - Nikolić, Dragana
AU  - Samardžić, Jelena
AU  - Maksimović, Vesna R.
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/496
AB  - BY-2, ćelijska linija duvana, upotrebljena je za produkciju novog tipa aspartatne proteinaze heljde - FeAPL1-His6. Potvrđena je očekivana pepstatin-A senzitivna enzimska aktivnost ovog proteina na pH 3,0. Lokalizacija proteina FeAPL1-His6 je određena imunocitohemijski i uporednom analizom Western blot transformisanih ćelija BY-2 i njihovih protoplasta. Ovim metodama je utvrđeno da ispitivani protein ima ekstracelularnu lokalizaciju u ćelijskom zidu. Na osnovu dobijenih rezultata razmatrana je potencijalna funkcija proteaze FeAPL1.
AB  - The recombinant aspartic proteinase-like protein (FeAPL1-His6) was overexpressed in the tobacco BY-2 cell line and the expected pepstatin A-sensitive enzymatic activity was confirmed at pH 3.0. Immunocytochemistry and protein gel blot analysis of the transformed BY-2 cells and their protoplasts showed extracellular localization of rFeAPL1-His6 in the cell wall. Based on the obtained results, potential functions of FeAPL1 are discussed.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Ćelijska lokalizacija novog tipa aspartatne proteinaze heljde (FeAPL1) u transformisanim BY-2 ćelijama duvana
T1  - Cell wall localization of the aspartic proteinase from buckwheat (FeAPL1) over-expressed in tobacco BY-2 cells
EP  - 1236
IS  - 9
SP  - 1229
VL  - 76
DO  - 10.2298/JSC110120106M
ER  - 

@article{
author = "Milisavljević, Mira and Timotijević, Gordana and Nikolić, Dragana and Samardžić, Jelena and Maksimović, Vesna R.",
year = "2011",
abstract = "BY-2, ćelijska linija duvana, upotrebljena je za produkciju novog tipa aspartatne proteinaze heljde - FeAPL1-His6. Potvrđena je očekivana pepstatin-A senzitivna enzimska aktivnost ovog proteina na pH 3,0. Lokalizacija proteina FeAPL1-His6 je određena imunocitohemijski i uporednom analizom Western blot transformisanih ćelija BY-2 i njihovih protoplasta. Ovim metodama je utvrđeno da ispitivani protein ima ekstracelularnu lokalizaciju u ćelijskom zidu. Na osnovu dobijenih rezultata razmatrana je potencijalna funkcija proteaze FeAPL1., The recombinant aspartic proteinase-like protein (FeAPL1-His6) was overexpressed in the tobacco BY-2 cell line and the expected pepstatin A-sensitive enzymatic activity was confirmed at pH 3.0. Immunocytochemistry and protein gel blot analysis of the transformed BY-2 cells and their protoplasts showed extracellular localization of rFeAPL1-His6 in the cell wall. Based on the obtained results, potential functions of FeAPL1 are discussed.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Ćelijska lokalizacija novog tipa aspartatne proteinaze heljde (FeAPL1) u transformisanim BY-2 ćelijama duvana, Cell wall localization of the aspartic proteinase from buckwheat (FeAPL1) over-expressed in tobacco BY-2 cells",
pages = "1236-1229",
number = "9",
volume = "76",
doi = "10.2298/JSC110120106M"
}

Milisavljević, M., Timotijević, G., Nikolić, D., Samardžić, J.,& Maksimović, V. R.. (2011). Ćelijska lokalizacija novog tipa aspartatne proteinaze heljde (FeAPL1) u transformisanim BY-2 ćelijama duvana. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 76(9), 1229-1236.
https://doi.org/10.2298/JSC110120106M

Milisavljević M, Timotijević G, Nikolić D, Samardžić J, Maksimović VR. Ćelijska lokalizacija novog tipa aspartatne proteinaze heljde (FeAPL1) u transformisanim BY-2 ćelijama duvana. in Journal of the Serbian Chemical Society. 2011;76(9):1229-1236.
doi:10.2298/JSC110120106M .

Milisavljević, Mira, Timotijević, Gordana, Nikolić, Dragana, Samardžić, Jelena, Maksimović, Vesna R., "Ćelijska lokalizacija novog tipa aspartatne proteinaze heljde (FeAPL1) u transformisanim BY-2 ćelijama duvana" in Journal of the Serbian Chemical Society, 76, no. 9 (2011):1229-1236,
https://doi.org/10.2298/JSC110120106M . .

TY  - JOUR
AU  - Timotijević, Gordana
AU  - Milisavljević, Mira
AU  - Radović, Svetlana R.
AU  - Konstantinović, Miroslav M.
AU  - Maksimović, Vesna R.
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/409
AB  - The aspartic protease (FeAP9) gene from buckwheat resembles the exon-intron structure characteristic for typical aspartic proteinases, including the presence of the leader intron in the 5'-UTR. RT PCR experiments and gel protein blot analysis indicated that FeAP9 was present in all analyzed organs: developing seeds, seedlings, flowers, leaves, roots and stems. Using Real-time PCR, we found that FeAP9 expression is upregulated in buckwheat leaves under the influence of different abiotic stresses, including dark, drought and UV-B light, as well as wounding and salicylic acid.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Plant Physiology
T1  - Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat
EP  - 68
IS  - 1
SP  - 61
VL  - 167
DO  - 10.1016/j.jplph.2009.06.017
ER  - 

@article{
author = "Timotijević, Gordana and Milisavljević, Mira and Radović, Svetlana R. and Konstantinović, Miroslav M. and Maksimović, Vesna R.",
year = "2010",
abstract = "The aspartic protease (FeAP9) gene from buckwheat resembles the exon-intron structure characteristic for typical aspartic proteinases, including the presence of the leader intron in the 5'-UTR. RT PCR experiments and gel protein blot analysis indicated that FeAP9 was present in all analyzed organs: developing seeds, seedlings, flowers, leaves, roots and stems. Using Real-time PCR, we found that FeAP9 expression is upregulated in buckwheat leaves under the influence of different abiotic stresses, including dark, drought and UV-B light, as well as wounding and salicylic acid.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Plant Physiology",
title = "Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat",
pages = "68-61",
number = "1",
volume = "167",
doi = "10.1016/j.jplph.2009.06.017"
}

Timotijević, G., Milisavljević, M., Radović, S. R., Konstantinović, M. M.,& Maksimović, V. R.. (2010). Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat. in Journal of Plant Physiology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 167(1), 61-68.
https://doi.org/10.1016/j.jplph.2009.06.017

Timotijević G, Milisavljević M, Radović SR, Konstantinović MM, Maksimović VR. Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat. in Journal of Plant Physiology. 2010;167(1):61-68.
doi:10.1016/j.jplph.2009.06.017 .

Timotijević, Gordana, Milisavljević, Mira, Radović, Svetlana R., Konstantinović, Miroslav M., Maksimović, Vesna R., "Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat" in Journal of Plant Physiology, 167, no. 1 (2010):61-68,
https://doi.org/10.1016/j.jplph.2009.06.017 . .

TY  - JOUR
AU  - Samardžić, Jelena
AU  - Nikolić, Dragana
AU  - Timotijević, Gordana
AU  - Jovanović, Živko
AU  - Milisavljević, Mira
AU  - Maksimović, Vesna R.
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/436
AB  - Metallothionein type 3 (MT3) expression has previously been detected in leaves fruits and developing somatic embryos in different plant species However specific tissular and cellular localization of MT3 transcripts have remained unidentified In this study in situ RNA-RNA analysis revealed buckwheat metallothionein type 3 (FeMT3) transcript localization in vascular elements mesophyll and guard cells of leaves vascular tissue of roots and throughout the whole embryo Changes in FeMT3 mRNA levels in response to drought and oxidative stress as well as ROS scavenging abilities of the FeMT3 protein in yeast were also detected indicating possible involvement of FeMT3 in stress defense and ROS related cellular processes
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Plant Physiology
T1  - Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum)
EP  - 1411
IS  - 16
SP  - 1407
VL  - 167
DO  - 10.1016/j.jplph.2010.05.016
ER  - 

@article{
author = "Samardžić, Jelena and Nikolić, Dragana and Timotijević, Gordana and Jovanović, Živko and Milisavljević, Mira and Maksimović, Vesna R.",
year = "2010",
abstract = "Metallothionein type 3 (MT3) expression has previously been detected in leaves fruits and developing somatic embryos in different plant species However specific tissular and cellular localization of MT3 transcripts have remained unidentified In this study in situ RNA-RNA analysis revealed buckwheat metallothionein type 3 (FeMT3) transcript localization in vascular elements mesophyll and guard cells of leaves vascular tissue of roots and throughout the whole embryo Changes in FeMT3 mRNA levels in response to drought and oxidative stress as well as ROS scavenging abilities of the FeMT3 protein in yeast were also detected indicating possible involvement of FeMT3 in stress defense and ROS related cellular processes",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Plant Physiology",
title = "Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum)",
pages = "1411-1407",
number = "16",
volume = "167",
doi = "10.1016/j.jplph.2010.05.016"
}

Samardžić, J., Nikolić, D., Timotijević, G., Jovanović, Ž., Milisavljević, M.,& Maksimović, V. R.. (2010). Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum). in Journal of Plant Physiology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 167(16), 1407-1411.
https://doi.org/10.1016/j.jplph.2010.05.016

Samardžić J, Nikolić D, Timotijević G, Jovanović Ž, Milisavljević M, Maksimović VR. Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum). in Journal of Plant Physiology. 2010;167(16):1407-1411.
doi:10.1016/j.jplph.2010.05.016 .

Samardžić, Jelena, Nikolić, Dragana, Timotijević, Gordana, Jovanović, Živko, Milisavljević, Mira, Maksimović, Vesna R., "Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum)" in Journal of Plant Physiology, 167, no. 16 (2010):1407-1411,
https://doi.org/10.1016/j.jplph.2010.05.016 . .

TY  - JOUR
AU  - Timotijević, Gordana
AU  - Milisavljević, Mira
AU  - Radović, Svetlana R.
AU  - Konstantinović, M.M.
AU  - Maksimović, Vesna R.
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/429
AB  - Gen za aspartičnu proteinazu (FeAP12) je izolovan iz eDNA biblioteke semena heljde u razviću. Analiza izvedene amino kiselinske sekvence FeAP12 gena ukazuje na njenu visoku homologiju sa ostalim tipičnim biljnim aspartičnim proteinazama (AP) koje se odlikuju prisustvom biljno specifičnog inserta (plant specific insert PSI), jedinstvenog među AP. Pokazano je da gen FeAP12 nije eksprimiran u listu, korenu, stablu i cvetu, već da je iRNA za FeAP12 prisutna samo u semenu. Najveći nivo ekspresije ovog gena je uočen u ranim fazama razvića semena, što ukazuje na njegovu moguću ulogu u degradaciji nucelusa.
AB  - Aspartic proteinase gene (FeAP12) has been isolated from the cDNA library of developing buckwheat seeds. Analysis of its deduced amino acid sequence showed that it resembled the structure and shared high homology with typical plant aspartic proteinases (AP) characterized by the presence of a plant-specific insert (PSI), unique among APs. It was shown that FeAP12 mRNA was not present in the leaves, roots, steam and flowers, but was seed-specifically expressed. Moreover, the highest levels of FeAP12 expression were observed in the early stages of seed development, therefore suggesting its potential role in nucellar degradation.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench)
T1  - Seed-specific aspartic proteinase FeAP12 from buckwheat (Fagopyrum esculentum Moench)
EP  - 151
IS  - 1
SP  - 143
VL  - 62
DO  - 10.2298/ABS1001143T
ER  - 

@article{
author = "Timotijević, Gordana and Milisavljević, Mira and Radović, Svetlana R. and Konstantinović, M.M. and Maksimović, Vesna R.",
year = "2010",
abstract = "Gen za aspartičnu proteinazu (FeAP12) je izolovan iz eDNA biblioteke semena heljde u razviću. Analiza izvedene amino kiselinske sekvence FeAP12 gena ukazuje na njenu visoku homologiju sa ostalim tipičnim biljnim aspartičnim proteinazama (AP) koje se odlikuju prisustvom biljno specifičnog inserta (plant specific insert PSI), jedinstvenog među AP. Pokazano je da gen FeAP12 nije eksprimiran u listu, korenu, stablu i cvetu, već da je iRNA za FeAP12 prisutna samo u semenu. Najveći nivo ekspresije ovog gena je uočen u ranim fazama razvića semena, što ukazuje na njegovu moguću ulogu u degradaciji nucelusa., Aspartic proteinase gene (FeAP12) has been isolated from the cDNA library of developing buckwheat seeds. Analysis of its deduced amino acid sequence showed that it resembled the structure and shared high homology with typical plant aspartic proteinases (AP) characterized by the presence of a plant-specific insert (PSI), unique among APs. It was shown that FeAP12 mRNA was not present in the leaves, roots, steam and flowers, but was seed-specifically expressed. Moreover, the highest levels of FeAP12 expression were observed in the early stages of seed development, therefore suggesting its potential role in nucellar degradation.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench), Seed-specific aspartic proteinase FeAP12 from buckwheat (Fagopyrum esculentum Moench)",
pages = "151-143",
number = "1",
volume = "62",
doi = "10.2298/ABS1001143T"
}

Timotijević, G., Milisavljević, M., Radović, S. R., Konstantinović, M.M.,& Maksimović, V. R.. (2010). Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench). in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(1), 143-151.
https://doi.org/10.2298/ABS1001143T

Timotijević G, Milisavljević M, Radović SR, Konstantinović M, Maksimović VR. Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench). in Archives of Biological Sciences. 2010;62(1):143-151.
doi:10.2298/ABS1001143T .

Timotijević, Gordana, Milisavljević, Mira, Radović, Svetlana R., Konstantinović, M.M., Maksimović, Vesna R., "Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench)" in Archives of Biological Sciences, 62, no. 1 (2010):143-151,
https://doi.org/10.2298/ABS1001143T . .