TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Mark, Kevin J.
AU  - Dragičević, Sandra
AU  - Babić, Tamara
AU  - Milošević, Katarina
AU  - Nestorović, Branimir
AU  - Beskoski, Vladimir
PY  - 2023
UR  - https://www.eurekaselect.com/article/129606
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2058
AB  - Background: Leukocytes are key cellular effectors of inflammation in asthma and understandingtheir function in this disease is of crucial importance. Blood leukocytes reflect the actions oftheir counterparts in the lungs and they can be obtained through minimal invasive procedures as partof the peripheral blood.Objective: The aim of the study was to identify proteins in blood leukocyte proteomes that respond toex vivo treatment by prednisone in order to pinpoint candidates for predictive biomarkers in corticosteroid-responsive asthma.Methods: The study included five children diagnosed with asthma and five healthy children. After theex vivo treatment of blood samples with prednisone, lysis of erythrocytes was performed and proteinswere extracted from the remaining leukocytes by ultrasonic disintegration. Protein extracts were analyzedby reversed phase nano-liquidchromatography–tandem mass spectrometry (LC–MS/MS).Results: The stimulation of asthmatics' leukocytes with prednisone has led to an increase in the levelsof FYB (fold change 3.4) and LYZ (fold change 2.2) with a statistical significance of p
T2  - Current Proteomics
T1  - Pilot Study of the Ex Vivo Blood Leukocytes’ Proteomic Response to Prednisone Stimulation in Corticosteroid-responsive Asthma
EP  - 18
IS  - 1
SP  - 12
VL  - 20
DO  - 10.2174/1570164620666230220112500
ER  - 

@article{
author = "Nikolić, Aleksandra and Mark, Kevin J. and Dragičević, Sandra and Babić, Tamara and Milošević, Katarina and Nestorović, Branimir and Beskoski, Vladimir",
year = "2023",
abstract = "Background: Leukocytes are key cellular effectors of inflammation in asthma and understandingtheir function in this disease is of crucial importance. Blood leukocytes reflect the actions oftheir counterparts in the lungs and they can be obtained through minimal invasive procedures as partof the peripheral blood.Objective: The aim of the study was to identify proteins in blood leukocyte proteomes that respond toex vivo treatment by prednisone in order to pinpoint candidates for predictive biomarkers in corticosteroid-responsive asthma.Methods: The study included five children diagnosed with asthma and five healthy children. After theex vivo treatment of blood samples with prednisone, lysis of erythrocytes was performed and proteinswere extracted from the remaining leukocytes by ultrasonic disintegration. Protein extracts were analyzedby reversed phase nano-liquidchromatography–tandem mass spectrometry (LC–MS/MS).Results: The stimulation of asthmatics' leukocytes with prednisone has led to an increase in the levelsof FYB (fold change 3.4) and LYZ (fold change 2.2) with a statistical significance of p",
journal = "Current Proteomics",
title = "Pilot Study of the Ex Vivo Blood Leukocytes’ Proteomic Response to Prednisone Stimulation in Corticosteroid-responsive Asthma",
pages = "18-12",
number = "1",
volume = "20",
doi = "10.2174/1570164620666230220112500"
}

Nikolić, A., Mark, K. J., Dragičević, S., Babić, T., Milošević, K., Nestorović, B.,& Beskoski, V.. (2023). Pilot Study of the Ex Vivo Blood Leukocytes’ Proteomic Response to Prednisone Stimulation in Corticosteroid-responsive Asthma. in Current Proteomics, 20(1), 12-18.
https://doi.org/10.2174/1570164620666230220112500

Nikolić A, Mark KJ, Dragičević S, Babić T, Milošević K, Nestorović B, Beskoski V. Pilot Study of the Ex Vivo Blood Leukocytes’ Proteomic Response to Prednisone Stimulation in Corticosteroid-responsive Asthma. in Current Proteomics. 2023;20(1):12-18.
doi:10.2174/1570164620666230220112500 .

Nikolić, Aleksandra, Mark, Kevin J., Dragičević, Sandra, Babić, Tamara, Milošević, Katarina, Nestorović, Branimir, Beskoski, Vladimir, "Pilot Study of the Ex Vivo Blood Leukocytes’ Proteomic Response to Prednisone Stimulation in Corticosteroid-responsive Asthma" in Current Proteomics, 20, no. 1 (2023):12-18,
https://doi.org/10.2174/1570164620666230220112500 . .

TY  - JOUR
AU  - Milošević, Katarina
AU  - Rsovac, Snežana
AU  - Nestorović, Branimir
AU  - Nikolić, Aleksandra
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2319
AB  - INTRODUCTION: Several studies have identified neuromuscular blocking agents as the most common cause of
anaphylaxis during general anesthesia. The reported data vary considerably between countries.
AIM: In this study we have investigated anaphylactic reactions to neuromuscular blocking agents, general
anaesthetics and latex in children from Serbia.
METHODS: This case series study has encompassed hypersensitivity testing in 78 children over a period of twelve
consecutive months. The diagnostic protocol consisted of a case history, immunoglobulin E (IgE) testing and skin
tests for hypersensitivity to neuromuscular blocking agents, general anesthetics and latex.
RESULTS: Of 78 children, 63% were hypersensitive to neuromuscular blocking agents, 28% to latex, 13% to
hypnotics, 10% to opioids and 15% to coloids. Sixty cases (77%) of anaphylaxis were IgE-mediated. Among
children who underwent more than one intervention, the presence of cross-sensibilisation for neuromuscular
blocking agents commercially available in Serbia was identified in 77% of cases.
CONCLUSION: Neuromuscular blocking agents were the most frequent causal agents of anaphylaxis in Serbian
children, as verified by skin tests and specific IgE tests. High frequencies of allergies to neuromuscular blocking
agents and latex obtained in this study underscore the importance of carrying out an allergy study to detect an
allergic cause in perioperative reactions
AB  - UVOD: Nekoliko studija je identifikovalo neuromuskularne blokatore kao najčešći uzrok anafilakse tokom opšte
anestezije, ali prijavljeni podaci znatno variraju između zemalja.
CILJ: U ovoj studiji ispitivane su anafilaktičke reakcije na neuromuskularne blokatore, opšte anestetike i lateks
kod dece iz Srbije.
METODE: Ova studija serije slučajeva obuhvatila je testiranje preosetljivosti kod 78 dece tokom perioda od
dvanaest uzastopnih meseci. Dijagnostički protokol se sastojao od istorije slučajeva, testiranja imunoglobulina E
(IgE) i kožnih testova za preosetljivost na neuromuskularne blokatore, opšte anestetike i lateks.
REZULTATI: Od 78 ispitivane dece, 63% je preosetljivo na neuromuskularne blokatore, 28% na lateks, 13% na
hipnotike, 10% na opioide i 15% na koloide. U 60 slučajeva (77%) anafilaksija je posredovana IgE-om. Među
decom koja su prošla više od jedne intervencije, prisustvo unakrsne senzibilizacije za neuromuskularne bokatore
komercijalno dostupne u Srbiji identifikovano je u 77% slučajeva.
ZAKLJUČAK: Neuromuskularni blokatori najčešći su uzročni agensi anafilaksije kod srpske dece, što je potvrđeno
testovima kože i specifičnim IgE testovima. Visoke učestalosti alergija na neuromuskularne blokatore i lateks
dobijene u ovoj studiji naglašavaju važnost sprovođenja studija alergije kako bi se otkrili alergijski uzroci u
perioperativnim reakcijama.
PB  - Novi Sad : Udruženje Medicina danas"
T2  - Medicina danas
T1  - Serbian study of perioperative anaphylaxis in children
T1  - Perioperativna anafilaksa kod dece u Srbiji
EP  - 180
IS  - 10-12
SP  - 175
VL  - 17
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2319
ER  - 

@article{
author = "Milošević, Katarina and Rsovac, Snežana and Nestorović, Branimir and Nikolić, Aleksandra",
year = "2018",
abstract = "INTRODUCTION: Several studies have identified neuromuscular blocking agents as the most common cause of
anaphylaxis during general anesthesia. The reported data vary considerably between countries.
AIM: In this study we have investigated anaphylactic reactions to neuromuscular blocking agents, general
anaesthetics and latex in children from Serbia.
METHODS: This case series study has encompassed hypersensitivity testing in 78 children over a period of twelve
consecutive months. The diagnostic protocol consisted of a case history, immunoglobulin E (IgE) testing and skin
tests for hypersensitivity to neuromuscular blocking agents, general anesthetics and latex.
RESULTS: Of 78 children, 63% were hypersensitive to neuromuscular blocking agents, 28% to latex, 13% to
hypnotics, 10% to opioids and 15% to coloids. Sixty cases (77%) of anaphylaxis were IgE-mediated. Among
children who underwent more than one intervention, the presence of cross-sensibilisation for neuromuscular
blocking agents commercially available in Serbia was identified in 77% of cases.
CONCLUSION: Neuromuscular blocking agents were the most frequent causal agents of anaphylaxis in Serbian
children, as verified by skin tests and specific IgE tests. High frequencies of allergies to neuromuscular blocking
agents and latex obtained in this study underscore the importance of carrying out an allergy study to detect an
allergic cause in perioperative reactions, UVOD: Nekoliko studija je identifikovalo neuromuskularne blokatore kao najčešći uzrok anafilakse tokom opšte
anestezije, ali prijavljeni podaci znatno variraju između zemalja.
CILJ: U ovoj studiji ispitivane su anafilaktičke reakcije na neuromuskularne blokatore, opšte anestetike i lateks
kod dece iz Srbije.
METODE: Ova studija serije slučajeva obuhvatila je testiranje preosetljivosti kod 78 dece tokom perioda od
dvanaest uzastopnih meseci. Dijagnostički protokol se sastojao od istorije slučajeva, testiranja imunoglobulina E
(IgE) i kožnih testova za preosetljivost na neuromuskularne blokatore, opšte anestetike i lateks.
REZULTATI: Od 78 ispitivane dece, 63% je preosetljivo na neuromuskularne blokatore, 28% na lateks, 13% na
hipnotike, 10% na opioide i 15% na koloide. U 60 slučajeva (77%) anafilaksija je posredovana IgE-om. Među
decom koja su prošla više od jedne intervencije, prisustvo unakrsne senzibilizacije za neuromuskularne bokatore
komercijalno dostupne u Srbiji identifikovano je u 77% slučajeva.
ZAKLJUČAK: Neuromuskularni blokatori najčešći su uzročni agensi anafilaksije kod srpske dece, što je potvrđeno
testovima kože i specifičnim IgE testovima. Visoke učestalosti alergija na neuromuskularne blokatore i lateks
dobijene u ovoj studiji naglašavaju važnost sprovođenja studija alergije kako bi se otkrili alergijski uzroci u
perioperativnim reakcijama.",
publisher = "Novi Sad : Udruženje Medicina danas"",
journal = "Medicina danas",
title = "Serbian study of perioperative anaphylaxis in children, Perioperativna anafilaksa kod dece u Srbiji",
pages = "180-175",
number = "10-12",
volume = "17",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2319"
}

Milošević, K., Rsovac, S., Nestorović, B.,& Nikolić, A.. (2018). Serbian study of perioperative anaphylaxis in children. in Medicina danas
Novi Sad : Udruženje Medicina danas"., 17(10-12), 175-180.
https://hdl.handle.net/21.15107/rcub_imagine_2319

Milošević K, Rsovac S, Nestorović B, Nikolić A. Serbian study of perioperative anaphylaxis in children. in Medicina danas. 2018;17(10-12):175-180.
https://hdl.handle.net/21.15107/rcub_imagine_2319 .

Milošević, Katarina, Rsovac, Snežana, Nestorović, Branimir, Nikolić, Aleksandra, "Serbian study of perioperative anaphylaxis in children" in Medicina danas, 17, no. 10-12 (2018):175-180,
https://hdl.handle.net/21.15107/rcub_imagine_2319 .

TY  - JOUR
AU  - Dragičević, Sandra
AU  - Milosević, Katarina
AU  - Nestorović, Branimir
AU  - Nikolić, Aleksandra
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1018
AB  - Transforming growth factor beta 1 (TGFB1) is a multifunctional cytokine with a key role in asthma airway inflammation and remodeling. Since elevated levels of this cytokine in airways might be associated with response to asthma therapy, the aim of this study was to investigate whether the presence of the polymorphism C-509T in the promoter of the TGFB1 gene is associated with response to montelukast. A group of 102 asthmatic patients was genotyped for the presence of the C-509T polymorphism by DNA sequencing and subjected to induced sputum sampling. Cells from sputum samples and BEAS 2B cells were treated with montelukast and endogenous TGFB1 expression was measured by quantitative real-time polymerase chain reaction. The promoter activity was analyzed by luciferase assays in BEAS 2B cells transfected with constructs carrying variants -509C and -509T of the TGFB1 gene promoter. After treatment with montelukast, the decrease in TGFB1 gene expression was greater for the -509TT genotype (58.9%) than for the -509CC and -509CT genotypes (49.6% and 31.8%, respectively) (P=0.071). In BEAS 2B cells, expression of endogenous TGFB1 was reduced by about 27% after montelukast treatment, while luciferase activity of both promoter variants was increased after montelukast treatment (-509C allele: 48.3%, P=0.060; and -509T allele: 100.5%, P=0.062). A more intensive response was registered in the promoter containing the -509T allele, which had 135% higher activity than the -509C variant (P=0.035). This study showed that the presence of the -509T allele in the TGFB1 promoter might modulate effects of montelukast on TGFB1 gene expression, but future studies are necessary, taking into consideration other genetic and nongenetic factors. It is of potential importance for clinical management of asthma to clarify the influence of the C-509T polymorphism on the response to treatment with montelukast.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Pediatric Allergy Immunology and Pulmonology
T1  - Influence of the Polymorphism C-509T in the TGFB1 Gene Promoter on the Response to Montelukast
EP  - 245
IS  - 4
SP  - 239
VL  - 30
DO  - 10.1089/ped.2017.0770
ER  - 

@article{
author = "Dragičević, Sandra and Milosević, Katarina and Nestorović, Branimir and Nikolić, Aleksandra",
year = "2017",
abstract = "Transforming growth factor beta 1 (TGFB1) is a multifunctional cytokine with a key role in asthma airway inflammation and remodeling. Since elevated levels of this cytokine in airways might be associated with response to asthma therapy, the aim of this study was to investigate whether the presence of the polymorphism C-509T in the promoter of the TGFB1 gene is associated with response to montelukast. A group of 102 asthmatic patients was genotyped for the presence of the C-509T polymorphism by DNA sequencing and subjected to induced sputum sampling. Cells from sputum samples and BEAS 2B cells were treated with montelukast and endogenous TGFB1 expression was measured by quantitative real-time polymerase chain reaction. The promoter activity was analyzed by luciferase assays in BEAS 2B cells transfected with constructs carrying variants -509C and -509T of the TGFB1 gene promoter. After treatment with montelukast, the decrease in TGFB1 gene expression was greater for the -509TT genotype (58.9%) than for the -509CC and -509CT genotypes (49.6% and 31.8%, respectively) (P=0.071). In BEAS 2B cells, expression of endogenous TGFB1 was reduced by about 27% after montelukast treatment, while luciferase activity of both promoter variants was increased after montelukast treatment (-509C allele: 48.3%, P=0.060; and -509T allele: 100.5%, P=0.062). A more intensive response was registered in the promoter containing the -509T allele, which had 135% higher activity than the -509C variant (P=0.035). This study showed that the presence of the -509T allele in the TGFB1 promoter might modulate effects of montelukast on TGFB1 gene expression, but future studies are necessary, taking into consideration other genetic and nongenetic factors. It is of potential importance for clinical management of asthma to clarify the influence of the C-509T polymorphism on the response to treatment with montelukast.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Pediatric Allergy Immunology and Pulmonology",
title = "Influence of the Polymorphism C-509T in the TGFB1 Gene Promoter on the Response to Montelukast",
pages = "245-239",
number = "4",
volume = "30",
doi = "10.1089/ped.2017.0770"
}

Dragičević, S., Milosević, K., Nestorović, B.,& Nikolić, A.. (2017). Influence of the Polymorphism C-509T in the TGFB1 Gene Promoter on the Response to Montelukast. in Pediatric Allergy Immunology and Pulmonology
Mary Ann Liebert, Inc, New Rochelle., 30(4), 239-245.
https://doi.org/10.1089/ped.2017.0770

Dragičević S, Milosević K, Nestorović B, Nikolić A. Influence of the Polymorphism C-509T in the TGFB1 Gene Promoter on the Response to Montelukast. in Pediatric Allergy Immunology and Pulmonology. 2017;30(4):239-245.
doi:10.1089/ped.2017.0770 .

Dragičević, Sandra, Milosević, Katarina, Nestorović, Branimir, Nikolić, Aleksandra, "Influence of the Polymorphism C-509T in the TGFB1 Gene Promoter on the Response to Montelukast" in Pediatric Allergy Immunology and Pulmonology, 30, no. 4 (2017):239-245,
https://doi.org/10.1089/ped.2017.0770 . .

TY  - JOUR
AU  - Rsovac, Snežana
AU  - Milosević, Katarina
AU  - Nestorović, Branimir
AU  - Nikolić, Aleksandra
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/786
AB  - Background. Mechanical ventilation is a frequently applied therapy in critically ill children and can be lifesaving in many cases. Clinical use of this technique has well documented benefits, but can be associated with different complications and adverse physiologic effects. Objectives. The aim of this study was to investigate the complications and clinical outcome of mechanical ventilation in Serbian pediatric patients. Material and Methods. The study encompassed 42 children with respiratory insufficiency that underwent mechanical ventilation during hospitalization over a period of 12 consecutive months. The influence of clinical and mechanical parameters on the occurrence of complications and clinical outcome were analyzed. Results. The patients were ventilated for a total of 432 days and a total of 61 complications were observed in 42 patients (97 complications per 1000 ventilation days). The most common complications associated with mechanical ventilation in Serbian pediatric patients with respiratory insufficiency were cardiovascular insufficiency (52.4%) and multiple organ failure (35.7%). High values of applied PIP ( gt 26 cm H2O), PEEP ( gt 6 cm H2O) and Tv ( gt 6 mL/kg) were associated with the occurrence of complications and negative clinical outcome. Conclusions. Complications of mechanical ventilation in the pediatric population occur frequently, but lower volumes/pressures of ventilation contribute to a decrease in incidence. Further studies are needed to examine associated risk factors and strategies to reduce the occurrence of complications and improve clinical outcome.
PB  - Wroclaw Medical Univ, Wroclaw
T2  - Advances in Clinical and Experimental Medicine
T1  - Complications of Mechanical Ventilation in Pediatric Patients in Serbia
EP  - 61
IS  - 1
SP  - 57
VL  - 23
DO  - 10.17219/acem/37022
ER  - 

@article{
author = "Rsovac, Snežana and Milosević, Katarina and Nestorović, Branimir and Nikolić, Aleksandra",
year = "2014",
abstract = "Background. Mechanical ventilation is a frequently applied therapy in critically ill children and can be lifesaving in many cases. Clinical use of this technique has well documented benefits, but can be associated with different complications and adverse physiologic effects. Objectives. The aim of this study was to investigate the complications and clinical outcome of mechanical ventilation in Serbian pediatric patients. Material and Methods. The study encompassed 42 children with respiratory insufficiency that underwent mechanical ventilation during hospitalization over a period of 12 consecutive months. The influence of clinical and mechanical parameters on the occurrence of complications and clinical outcome were analyzed. Results. The patients were ventilated for a total of 432 days and a total of 61 complications were observed in 42 patients (97 complications per 1000 ventilation days). The most common complications associated with mechanical ventilation in Serbian pediatric patients with respiratory insufficiency were cardiovascular insufficiency (52.4%) and multiple organ failure (35.7%). High values of applied PIP ( gt 26 cm H2O), PEEP ( gt 6 cm H2O) and Tv ( gt 6 mL/kg) were associated with the occurrence of complications and negative clinical outcome. Conclusions. Complications of mechanical ventilation in the pediatric population occur frequently, but lower volumes/pressures of ventilation contribute to a decrease in incidence. Further studies are needed to examine associated risk factors and strategies to reduce the occurrence of complications and improve clinical outcome.",
publisher = "Wroclaw Medical Univ, Wroclaw",
journal = "Advances in Clinical and Experimental Medicine",
title = "Complications of Mechanical Ventilation in Pediatric Patients in Serbia",
pages = "61-57",
number = "1",
volume = "23",
doi = "10.17219/acem/37022"
}

Rsovac, S., Milosević, K., Nestorović, B.,& Nikolić, A.. (2014). Complications of Mechanical Ventilation in Pediatric Patients in Serbia. in Advances in Clinical and Experimental Medicine
Wroclaw Medical Univ, Wroclaw., 23(1), 57-61.
https://doi.org/10.17219/acem/37022

Rsovac S, Milosević K, Nestorović B, Nikolić A. Complications of Mechanical Ventilation in Pediatric Patients in Serbia. in Advances in Clinical and Experimental Medicine. 2014;23(1):57-61.
doi:10.17219/acem/37022 .

Rsovac, Snežana, Milosević, Katarina, Nestorović, Branimir, Nikolić, Aleksandra, "Complications of Mechanical Ventilation in Pediatric Patients in Serbia" in Advances in Clinical and Experimental Medicine, 23, no. 1 (2014):57-61,
https://doi.org/10.17219/acem/37022 . .

TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Milosević, Katarina
AU  - Bošković, Srđan
AU  - Nestorović, Branimir
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/709
AB  - The aim of this study was to investigate polymorphisms in the promoter region of the neutrophil elastase (ELANE) gene as potential modulators of the therapeutic response in children with idiopathic bronchiectasis. The study included 48 children between 5 and 17 years old who were diagnosed with idiopathic bronchiectasis based on high-resolution computed tomography of the thorax. In all patients therapy included administration of antibiotics, anti-inflammatory drugs, expectorants, and postural drainage. Response to therapy was evaluated by the change in FeNO levels before and after administration of therapy. The ELANE promoter region polymorphisms were analyzed by PCR-direct DNA sequencing. According to the predicted activity of ELANE genotypes, subjects were divided into two groups: low/intermediate activity (n = 18) and high activity (n = 30). Subjects in the group with high-activity genotype had higher initial FeNO levels and this difference was statistically significant (t = 2.906; p = 0.006). The difference between FeNO levels before and after therapy was also statistically significantly higher in children with high-activity genotype (t = 3.329; p = 0.002). Statistically significant correlation was observed between the change in FeNO levels and ELANE genotypes (r = 0.350; p = 0.015). Children with high-activity genotype had higher initial FeNO levels and showed better response to therapy than children with low/intermediate-activity genotypes.
PB  - Springer, New York
T2  - Lung
T1  - Neutrophil Elastase Gene Polymorphisms: Modulators of Response to Therapy in Childhood Bronchiectasis?
EP  - 599
IS  - 4
SP  - 595
VL  - 192
DO  - 10.1007/s00408-014-9596-3
ER  - 

@article{
author = "Nikolić, Aleksandra and Milosević, Katarina and Bošković, Srđan and Nestorović, Branimir",
year = "2014",
abstract = "The aim of this study was to investigate polymorphisms in the promoter region of the neutrophil elastase (ELANE) gene as potential modulators of the therapeutic response in children with idiopathic bronchiectasis. The study included 48 children between 5 and 17 years old who were diagnosed with idiopathic bronchiectasis based on high-resolution computed tomography of the thorax. In all patients therapy included administration of antibiotics, anti-inflammatory drugs, expectorants, and postural drainage. Response to therapy was evaluated by the change in FeNO levels before and after administration of therapy. The ELANE promoter region polymorphisms were analyzed by PCR-direct DNA sequencing. According to the predicted activity of ELANE genotypes, subjects were divided into two groups: low/intermediate activity (n = 18) and high activity (n = 30). Subjects in the group with high-activity genotype had higher initial FeNO levels and this difference was statistically significant (t = 2.906; p = 0.006). The difference between FeNO levels before and after therapy was also statistically significantly higher in children with high-activity genotype (t = 3.329; p = 0.002). Statistically significant correlation was observed between the change in FeNO levels and ELANE genotypes (r = 0.350; p = 0.015). Children with high-activity genotype had higher initial FeNO levels and showed better response to therapy than children with low/intermediate-activity genotypes.",
publisher = "Springer, New York",
journal = "Lung",
title = "Neutrophil Elastase Gene Polymorphisms: Modulators of Response to Therapy in Childhood Bronchiectasis?",
pages = "599-595",
number = "4",
volume = "192",
doi = "10.1007/s00408-014-9596-3"
}

Nikolić, A., Milosević, K., Bošković, S.,& Nestorović, B.. (2014). Neutrophil Elastase Gene Polymorphisms: Modulators of Response to Therapy in Childhood Bronchiectasis?. in Lung
Springer, New York., 192(4), 595-599.
https://doi.org/10.1007/s00408-014-9596-3

Nikolić A, Milosević K, Bošković S, Nestorović B. Neutrophil Elastase Gene Polymorphisms: Modulators of Response to Therapy in Childhood Bronchiectasis?. in Lung. 2014;192(4):595-599.
doi:10.1007/s00408-014-9596-3 .

Nikolić, Aleksandra, Milosević, Katarina, Bošković, Srđan, Nestorović, Branimir, "Neutrophil Elastase Gene Polymorphisms: Modulators of Response to Therapy in Childhood Bronchiectasis?" in Lung, 192, no. 4 (2014):595-599,
https://doi.org/10.1007/s00408-014-9596-3 . .

TY  - JOUR
AU  - Nestorović, Branimir
AU  - Milosević, Katarina
AU  - Rsovac, Snežana
AU  - Nikolić, Aleksandra
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/636
AB  - A 36-year-old woman was stung in the right wrist by a bee, suffered typical anaphylaxis, and was transferred to a local hospital. After a few hours, which corresponded to late-onset reaction, she developed shortness of breath and weakness and was transferred to the emergency department, where the diagnosis of anaphylaxis was confirmed. Serum complement levels, components C3 and C4, were undetectable. Flexible bronchoscopy excluded lung hemorrhage. She was on the ventilator for 4 days and was fully awake during that time. After the treatment, her improvement was rapid, and she fully recovered. Three weeks after she had been stung by a bee, skin prick tests to bee, wasp, and yellow jacket venom were done, and all tests came back negative as well as IgE and IgG4 antibodies to the same venoms. Coagulation factors and the complement, including C1q inhibitor, were normal. Occurrence of complement activation and consumption could point to the immune complexes as basis of pathophysiological mechanism. It remains unclear why such a reaction would involve only the right lung and why no detectable immune complexes were discovered. The clinical picture in the presented case resembled acute respiratory distress syndrome, but the exact nature of lung consolidation remains puzzling. The most likely explanation is a very rare case of airway obstruction. To our knowledge, this is the only reported case of anaphylaxis associated with undetectable serum complement levels. The potential role and diagnostic significance of hypocomplementemia in cases of anaphylaxis should be further investigated.
PB  - W B Saunders Co-Elsevier Inc, Philadelphia
T2  - American Journal of Emergency Medicine
T1  - Anaphylaxis followed by unilateral lung opacity and hypocomplementemia in a young female
IS  - 11
VL  - 31
DO  - 10.1016/j.ajem.2013.06.025
ER  - 

@article{
author = "Nestorović, Branimir and Milosević, Katarina and Rsovac, Snežana and Nikolić, Aleksandra",
year = "2013",
abstract = "A 36-year-old woman was stung in the right wrist by a bee, suffered typical anaphylaxis, and was transferred to a local hospital. After a few hours, which corresponded to late-onset reaction, she developed shortness of breath and weakness and was transferred to the emergency department, where the diagnosis of anaphylaxis was confirmed. Serum complement levels, components C3 and C4, were undetectable. Flexible bronchoscopy excluded lung hemorrhage. She was on the ventilator for 4 days and was fully awake during that time. After the treatment, her improvement was rapid, and she fully recovered. Three weeks after she had been stung by a bee, skin prick tests to bee, wasp, and yellow jacket venom were done, and all tests came back negative as well as IgE and IgG4 antibodies to the same venoms. Coagulation factors and the complement, including C1q inhibitor, were normal. Occurrence of complement activation and consumption could point to the immune complexes as basis of pathophysiological mechanism. It remains unclear why such a reaction would involve only the right lung and why no detectable immune complexes were discovered. The clinical picture in the presented case resembled acute respiratory distress syndrome, but the exact nature of lung consolidation remains puzzling. The most likely explanation is a very rare case of airway obstruction. To our knowledge, this is the only reported case of anaphylaxis associated with undetectable serum complement levels. The potential role and diagnostic significance of hypocomplementemia in cases of anaphylaxis should be further investigated.",
publisher = "W B Saunders Co-Elsevier Inc, Philadelphia",
journal = "American Journal of Emergency Medicine",
title = "Anaphylaxis followed by unilateral lung opacity and hypocomplementemia in a young female",
number = "11",
volume = "31",
doi = "10.1016/j.ajem.2013.06.025"
}

Nestorović, B., Milosević, K., Rsovac, S.,& Nikolić, A.. (2013). Anaphylaxis followed by unilateral lung opacity and hypocomplementemia in a young female. in American Journal of Emergency Medicine
W B Saunders Co-Elsevier Inc, Philadelphia., 31(11).
https://doi.org/10.1016/j.ajem.2013.06.025

Nestorović B, Milosević K, Rsovac S, Nikolić A. Anaphylaxis followed by unilateral lung opacity and hypocomplementemia in a young female. in American Journal of Emergency Medicine. 2013;31(11).
doi:10.1016/j.ajem.2013.06.025 .

Nestorović, Branimir, Milosević, Katarina, Rsovac, Snežana, Nikolić, Aleksandra, "Anaphylaxis followed by unilateral lung opacity and hypocomplementemia in a young female" in American Journal of Emergency Medicine, 31, no. 11 (2013),
https://doi.org/10.1016/j.ajem.2013.06.025 . .

TY  - JOUR
AU  - Milosević, Katarina
AU  - Nikolić, Aleksandra
AU  - Divac Rankov, Aleksandra
AU  - Ljujić, Mila
AU  - Nestorović, Branimir
AU  - Radojković, Dragica
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/652
AB  - This study has investigated a potential role of common Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene variants in the etiology of noncystic fibrosis bronchiectasis in Serbian children. The study has encompassed 48 patients (19 male and 29 female, aged between 5 and 18 years, median age 10.6 +/- 3.3), diagnosed with idiopathic bronchiectasis based on high-resolution computed tomography of thorax and pathologic examination of lobectomy materials. The CFTR gene analysis was performed on genomic DNA extracted from peripheral blood samples of patients by polymerase chain reaction (PCR)-Mediated Site-Directed Mutagenesis method, Denaturing Gradient Gel Electrophoresis method, and DNA sequencing. Mutation c.1521_1523delCTT (F508del) was detected with an allelic frequency of 1.0%, and c.224G gt A (R75Q) variant. Carriers of c.1210-12T[5] (IVS8-5T) allele were significantly more common than in the general population (10.4% vs. 5.0%, P = 0.0302). The frequency of homozygotes for Met 470 allele was higher in patients than in the general population (33% vs. 20%), while heterozygotes for p. Met470Val were less frequent (31% vs. 50%), and this difference was statistically significant (P = 0.0222). The results obtained in this study indicate involvement of 2 common CFTR variants, c. 1210-12T[5] and c. 1408A, in idiopathic bronchiectasis in children, but this observation should be further confirmed by more extensive analysis of the CFTR gene in a larger group of patients.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Pediatric Allergy Immunology and Pulmonology
T1  - Analysis of CFTR Gene Variants in Idiopathic Bronchiectasis in Serbian Children
EP  - 98
IS  - 2
SP  - 93
VL  - 26
DO  - 10.1089/ped.2013.0238
ER  - 

@article{
author = "Milosević, Katarina and Nikolić, Aleksandra and Divac Rankov, Aleksandra and Ljujić, Mila and Nestorović, Branimir and Radojković, Dragica",
year = "2013",
abstract = "This study has investigated a potential role of common Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene variants in the etiology of noncystic fibrosis bronchiectasis in Serbian children. The study has encompassed 48 patients (19 male and 29 female, aged between 5 and 18 years, median age 10.6 +/- 3.3), diagnosed with idiopathic bronchiectasis based on high-resolution computed tomography of thorax and pathologic examination of lobectomy materials. The CFTR gene analysis was performed on genomic DNA extracted from peripheral blood samples of patients by polymerase chain reaction (PCR)-Mediated Site-Directed Mutagenesis method, Denaturing Gradient Gel Electrophoresis method, and DNA sequencing. Mutation c.1521_1523delCTT (F508del) was detected with an allelic frequency of 1.0%, and c.224G gt A (R75Q) variant. Carriers of c.1210-12T[5] (IVS8-5T) allele were significantly more common than in the general population (10.4% vs. 5.0%, P = 0.0302). The frequency of homozygotes for Met 470 allele was higher in patients than in the general population (33% vs. 20%), while heterozygotes for p. Met470Val were less frequent (31% vs. 50%), and this difference was statistically significant (P = 0.0222). The results obtained in this study indicate involvement of 2 common CFTR variants, c. 1210-12T[5] and c. 1408A, in idiopathic bronchiectasis in children, but this observation should be further confirmed by more extensive analysis of the CFTR gene in a larger group of patients.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Pediatric Allergy Immunology and Pulmonology",
title = "Analysis of CFTR Gene Variants in Idiopathic Bronchiectasis in Serbian Children",
pages = "98-93",
number = "2",
volume = "26",
doi = "10.1089/ped.2013.0238"
}

Milosević, K., Nikolić, A., Divac Rankov, A., Ljujić, M., Nestorović, B.,& Radojković, D.. (2013). Analysis of CFTR Gene Variants in Idiopathic Bronchiectasis in Serbian Children. in Pediatric Allergy Immunology and Pulmonology
Mary Ann Liebert, Inc, New Rochelle., 26(2), 93-98.
https://doi.org/10.1089/ped.2013.0238

Milosević K, Nikolić A, Divac Rankov A, Ljujić M, Nestorović B, Radojković D. Analysis of CFTR Gene Variants in Idiopathic Bronchiectasis in Serbian Children. in Pediatric Allergy Immunology and Pulmonology. 2013;26(2):93-98.
doi:10.1089/ped.2013.0238 .

Milosević, Katarina, Nikolić, Aleksandra, Divac Rankov, Aleksandra, Ljujić, Mila, Nestorović, Branimir, Radojković, Dragica, "Analysis of CFTR Gene Variants in Idiopathic Bronchiectasis in Serbian Children" in Pediatric Allergy Immunology and Pulmonology, 26, no. 2 (2013):93-98,
https://doi.org/10.1089/ped.2013.0238 . .

TY  - CONF
AU  - Milosević, Katarina
AU  - Bošković, Srđan
AU  - Ljujić, Mila
AU  - Nestorović, Branimir
AU  - Nikolić, Aleksandra
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/675
PB  - European Respiratory Soc Journals Ltd, Sheffield
C3  - European Respiratory Journal
T1  - Neutrophil elastase gene polymorphisms as therapy modulators in childhood bronchiectasis
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_imagine_675
ER  - 

@conference{
author = "Milosević, Katarina and Bošković, Srđan and Ljujić, Mila and Nestorović, Branimir and Nikolić, Aleksandra",
year = "2013",
publisher = "European Respiratory Soc Journals Ltd, Sheffield",
journal = "European Respiratory Journal",
title = "Neutrophil elastase gene polymorphisms as therapy modulators in childhood bronchiectasis",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_imagine_675"
}

Milosević, K., Bošković, S., Ljujić, M., Nestorović, B.,& Nikolić, A.. (2013). Neutrophil elastase gene polymorphisms as therapy modulators in childhood bronchiectasis. in European Respiratory Journal
European Respiratory Soc Journals Ltd, Sheffield., 42.
https://hdl.handle.net/21.15107/rcub_imagine_675

Milosević K, Bošković S, Ljujić M, Nestorović B, Nikolić A. Neutrophil elastase gene polymorphisms as therapy modulators in childhood bronchiectasis. in European Respiratory Journal. 2013;42.
https://hdl.handle.net/21.15107/rcub_imagine_675 .

Milosević, Katarina, Bošković, Srđan, Ljujić, Mila, Nestorović, Branimir, Nikolić, Aleksandra, "Neutrophil elastase gene polymorphisms as therapy modulators in childhood bronchiectasis" in European Respiratory Journal, 42 (2013),
https://hdl.handle.net/21.15107/rcub_imagine_675 .

TY  - JOUR
AU  - Milosević, Katarina
AU  - Rsovac, Snežana
AU  - Nestorović, Branimir
AU  - Nikolić, Aleksandra
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/567
AB  - The aim of this study was to investigate response to salbutamol in children with acute asthma exacerbations in order to estimate the efficiency and safety of its administration in pediatric population. The study included 56 children with asthma (age range 5-12) who were diagnosed and treated over a period of two years. In all children, salbutamol was administered via nebulization, in doses of 1.25 mg in children aged 5-6 and 2.5 mg in children aged 7-12. Respiratory and blood parameters were monitored before and 30 minutes after salbutamol administration. In children with acute asthma exacerbations, respiratory frequency, potassium level, sodium level and PaCO, decreased significantly after salbutamol administration, while PEF, PEF%, glycemia, PaO2 and SatO(2) significantly increased. Hypokalemia due to salbutanzol effect occurred in 15 (26.8%) children. Most significant improvement in oxygenation, with increase of PEF, PEF%, PaO2 and SatO(2), after salbutamol administration was achieved in patients with mild acute exacerbations. Salbulanzol administered via nebulization leads to the occurrence of adverse metabolic elfe cts, hypokalemia and hyperglycemia. Howevez; it can be safely used in the treatment of acute asthma exacerbations and demonstrates high clinical efficacy in the management of acute bronchospasm. The most effective salbutamol treatment is achieved in patients with mild exacerbations.
T2  - Paediatria Croatica
T1  - Response to salbutamol in children with acute asthma exacerbations
EP  - 208
IS  - 3
SP  - 205
VL  - 56
UR  - https://hdl.handle.net/21.15107/rcub_imagine_567
ER  - 

@article{
author = "Milosević, Katarina and Rsovac, Snežana and Nestorović, Branimir and Nikolić, Aleksandra",
year = "2012",
abstract = "The aim of this study was to investigate response to salbutamol in children with acute asthma exacerbations in order to estimate the efficiency and safety of its administration in pediatric population. The study included 56 children with asthma (age range 5-12) who were diagnosed and treated over a period of two years. In all children, salbutamol was administered via nebulization, in doses of 1.25 mg in children aged 5-6 and 2.5 mg in children aged 7-12. Respiratory and blood parameters were monitored before and 30 minutes after salbutamol administration. In children with acute asthma exacerbations, respiratory frequency, potassium level, sodium level and PaCO, decreased significantly after salbutamol administration, while PEF, PEF%, glycemia, PaO2 and SatO(2) significantly increased. Hypokalemia due to salbutanzol effect occurred in 15 (26.8%) children. Most significant improvement in oxygenation, with increase of PEF, PEF%, PaO2 and SatO(2), after salbutamol administration was achieved in patients with mild acute exacerbations. Salbulanzol administered via nebulization leads to the occurrence of adverse metabolic elfe cts, hypokalemia and hyperglycemia. Howevez; it can be safely used in the treatment of acute asthma exacerbations and demonstrates high clinical efficacy in the management of acute bronchospasm. The most effective salbutamol treatment is achieved in patients with mild exacerbations.",
journal = "Paediatria Croatica",
title = "Response to salbutamol in children with acute asthma exacerbations",
pages = "208-205",
number = "3",
volume = "56",
url = "https://hdl.handle.net/21.15107/rcub_imagine_567"
}

Milosević, K., Rsovac, S., Nestorović, B.,& Nikolić, A.. (2012). Response to salbutamol in children with acute asthma exacerbations. in Paediatria Croatica, 56(3), 205-208.
https://hdl.handle.net/21.15107/rcub_imagine_567

Milosević K, Rsovac S, Nestorović B, Nikolić A. Response to salbutamol in children with acute asthma exacerbations. in Paediatria Croatica. 2012;56(3):205-208.
https://hdl.handle.net/21.15107/rcub_imagine_567 .

Milosević, Katarina, Rsovac, Snežana, Nestorović, Branimir, Nikolić, Aleksandra, "Response to salbutamol in children with acute asthma exacerbations" in Paediatria Croatica, 56, no. 3 (2012):205-208,
https://hdl.handle.net/21.15107/rcub_imagine_567 .

TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Milosević, Katarina
AU  - Divac Rankov, Aleksandra
AU  - Ljujić, Mila
AU  - Grković, Slobodanka
AU  - Nestorović, Branimir
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/466
AB  - This paper reports a novel Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene variant, 1811+1G - gt  T, detected in a 5-year-old girl diagnosed with idiopathic disseminated bronchiectasis and negative sweat chloride test (17 mmol/L). The performed CFTR gene mutation analysis included detection of the F508del mutation, analysis of Tn polymorphism and screening of CFTR exons 3, 10 and 11. The CFTR gene screening has shown the altered band pattern in exon 11. The DNA sequencing of CFTR exon 11 revealed the presence of the novel sequence variation 1811+1G - gt  T in heterozygous state. This sequence variation was not found in any of 100 control alleles, analyzed by polymerase chain reaction - restriction fragment length polymorphism method. The novel sequence variation 1811+1G - gt  T is located at the splicing site at the boundary of exon 11 and intron 11 and might be either a sequence variation or a splicing site defect. lt /.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Fetal and Pediatric Pathology
T1  - Novel cftr gene sequence variation in Serbian patient with idiopathic disseminated bronchiectasis
EP  - 98
IS  - 2
SP  - 95
VL  - 29
DO  - 10.3109/15513811003620815
ER  - 

@article{
author = "Nikolić, Aleksandra and Milosević, Katarina and Divac Rankov, Aleksandra and Ljujić, Mila and Grković, Slobodanka and Nestorović, Branimir",
year = "2010",
abstract = "This paper reports a novel Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene variant, 1811+1G - gt  T, detected in a 5-year-old girl diagnosed with idiopathic disseminated bronchiectasis and negative sweat chloride test (17 mmol/L). The performed CFTR gene mutation analysis included detection of the F508del mutation, analysis of Tn polymorphism and screening of CFTR exons 3, 10 and 11. The CFTR gene screening has shown the altered band pattern in exon 11. The DNA sequencing of CFTR exon 11 revealed the presence of the novel sequence variation 1811+1G - gt  T in heterozygous state. This sequence variation was not found in any of 100 control alleles, analyzed by polymerase chain reaction - restriction fragment length polymorphism method. The novel sequence variation 1811+1G - gt  T is located at the splicing site at the boundary of exon 11 and intron 11 and might be either a sequence variation or a splicing site defect. lt /.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Fetal and Pediatric Pathology",
title = "Novel cftr gene sequence variation in Serbian patient with idiopathic disseminated bronchiectasis",
pages = "98-95",
number = "2",
volume = "29",
doi = "10.3109/15513811003620815"
}

Nikolić, A., Milosević, K., Divac Rankov, A., Ljujić, M., Grković, S.,& Nestorović, B.. (2010). Novel cftr gene sequence variation in Serbian patient with idiopathic disseminated bronchiectasis. in Fetal and Pediatric Pathology
Taylor & Francis Inc, Philadelphia., 29(2), 95-98.
https://doi.org/10.3109/15513811003620815

Nikolić A, Milosević K, Divac Rankov A, Ljujić M, Grković S, Nestorović B. Novel cftr gene sequence variation in Serbian patient with idiopathic disseminated bronchiectasis. in Fetal and Pediatric Pathology. 2010;29(2):95-98.
doi:10.3109/15513811003620815 .

Nikolić, Aleksandra, Milosević, Katarina, Divac Rankov, Aleksandra, Ljujić, Mila, Grković, Slobodanka, Nestorović, Branimir, "Novel cftr gene sequence variation in Serbian patient with idiopathic disseminated bronchiectasis" in Fetal and Pediatric Pathology, 29, no. 2 (2010):95-98,
https://doi.org/10.3109/15513811003620815 . .