TY  - JOUR
AU  - Malagurski, Ivana
AU  - Lazić, Jelena
AU  - Ilić-Tomić, Tatjana
AU  - Salevic, Ana
AU  - Guzik, Maciej
AU  - Krzan, Marcel
AU  - Nikodinović-Runić, Jasmina
AU  - Ponjavić, Marijana
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0141813024058938
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2500
AB  - Synthetic materials alternatives are crucial for reaching sustainable development goals and waste reduction. Biomaterials and biomolecules obtained through bacterial fermentation offer a viable solution. Double-layer active UV-blocking material composed of bacterial nanocellulose as an inner layer and poly(hydroxyoctanoic acid) containing prodigiosin as an active compound was produced by layer-by-layer deposition. This study referred the new material consisted of the three components produced in sustainable manner, by bacterial activity: bacterial bio-pigment prodigiosin, bacterial nanocellulose and poly(hydroytoctanoate) - biopolymer obtained by microbial fermentations. Prior the final double layer film was produced, PHO films containing different PG concentrations as a layer in charge of the bioactivity (0.2, 0.5 and 1 wt%) was casted and systematically characterized (FTIR, DSC, XRD, wettability, SEM, transparency, mechanical tests) to optimize their properties. The formulation with the best UV-blocking properties and less toxicity effect tested using MRC5 cells was chosen as an outer layer in double-layer films production. Water contact angle measurements confirmed that hydrophilic – hydrophobic double layer film was obtained with the improved mechanical properties in comparison to the native BNC. Migration test indicated release of PG in all tested media as a consequence of bilayer formulation, while the PG release from PHO in 10 % ethanol was not detected. All findings from the study suggested this activated, UV-blocking material as a candidate with excellent potential in packaging industry.
T2  - International Journal of Biological Macromolecules
T1  - Double layer bacterial nanocellulose - poly(hydroxyoctanoate) film activated by prodigiosin as sustainable, transparent, UV-blocking material
SP  - 135087
VL  - 279
DO  - 10.1016/j.ijbiomac.2024.135087
ER  - 

@article{
author = "Malagurski, Ivana and Lazić, Jelena and Ilić-Tomić, Tatjana and Salevic, Ana and Guzik, Maciej and Krzan, Marcel and Nikodinović-Runić, Jasmina and Ponjavić, Marijana",
year = "2024",
abstract = "Synthetic materials alternatives are crucial for reaching sustainable development goals and waste reduction. Biomaterials and biomolecules obtained through bacterial fermentation offer a viable solution. Double-layer active UV-blocking material composed of bacterial nanocellulose as an inner layer and poly(hydroxyoctanoic acid) containing prodigiosin as an active compound was produced by layer-by-layer deposition. This study referred the new material consisted of the three components produced in sustainable manner, by bacterial activity: bacterial bio-pigment prodigiosin, bacterial nanocellulose and poly(hydroytoctanoate) - biopolymer obtained by microbial fermentations. Prior the final double layer film was produced, PHO films containing different PG concentrations as a layer in charge of the bioactivity (0.2, 0.5 and 1 wt%) was casted and systematically characterized (FTIR, DSC, XRD, wettability, SEM, transparency, mechanical tests) to optimize their properties. The formulation with the best UV-blocking properties and less toxicity effect tested using MRC5 cells was chosen as an outer layer in double-layer films production. Water contact angle measurements confirmed that hydrophilic – hydrophobic double layer film was obtained with the improved mechanical properties in comparison to the native BNC. Migration test indicated release of PG in all tested media as a consequence of bilayer formulation, while the PG release from PHO in 10 % ethanol was not detected. All findings from the study suggested this activated, UV-blocking material as a candidate with excellent potential in packaging industry.",
journal = "International Journal of Biological Macromolecules",
title = "Double layer bacterial nanocellulose - poly(hydroxyoctanoate) film activated by prodigiosin as sustainable, transparent, UV-blocking material",
pages = "135087",
volume = "279",
doi = "10.1016/j.ijbiomac.2024.135087"
}

Malagurski, I., Lazić, J., Ilić-Tomić, T., Salevic, A., Guzik, M., Krzan, M., Nikodinović-Runić, J.,& Ponjavić, M.. (2024). Double layer bacterial nanocellulose - poly(hydroxyoctanoate) film activated by prodigiosin as sustainable, transparent, UV-blocking material. in International Journal of Biological Macromolecules, 279, 135087.
https://doi.org/10.1016/j.ijbiomac.2024.135087

Malagurski I, Lazić J, Ilić-Tomić T, Salevic A, Guzik M, Krzan M, Nikodinović-Runić J, Ponjavić M. Double layer bacterial nanocellulose - poly(hydroxyoctanoate) film activated by prodigiosin as sustainable, transparent, UV-blocking material. in International Journal of Biological Macromolecules. 2024;279:135087.
doi:10.1016/j.ijbiomac.2024.135087 .

Malagurski, Ivana, Lazić, Jelena, Ilić-Tomić, Tatjana, Salevic, Ana, Guzik, Maciej, Krzan, Marcel, Nikodinović-Runić, Jasmina, Ponjavić, Marijana, "Double layer bacterial nanocellulose - poly(hydroxyoctanoate) film activated by prodigiosin as sustainable, transparent, UV-blocking material" in International Journal of Biological Macromolecules, 279 (2024):135087,
https://doi.org/10.1016/j.ijbiomac.2024.135087 . .

TY  - JOUR
AU  - Skibiński, Szymon
AU  - Czechowska, Joanna P.
AU  - Milivojević, Dušan
AU  - Nikodinović-Runić, Jasmina
AU  - Guzik, Maciej
AU  - Zima, Aneta
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0272884224020467
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2390
AB  - The risk of bacterial infections is a significant challenge faced frequently in the use of implants or scaffolds for bone regeneration. Therefore, this study focusses on the development and characterisation of novel β tricalcium phosphate (βTCP) scaffolds co-doped with silver and silicon, along with composites coated with antibiotic-loaded poly(3-hydroxybutyrate) (P(3HB)) layers. The successful incorporation of silver and silicon dopants while maintaining the formation of βTCP phase was confirmed using X-ray fluorescence (XRF), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Raman spectroscopy, and scanning electron microscopy/energy-dispersive X-ray spectroscopy (SEM/EDS) analysis. The developed materials demonstrated comparable total and open porosity (∼64–70 vol%), suggesting high interconnectivity between pores conducive to nutrient transport and tissue repair. The increase in compressive strength was achieved for both doped (4.73 ± 0.79 MPa) and P(3HB) coated (5.79 ± 0.92 MPa) scaffolds due to the fine bioceramic microstructure and polymeric coating. Silver and silicon-modified βTCP demonstrated enhanced growth inhibition of Gram-negative (Pseudomonas aeruginosa, Escherichia coli) and Gram-positive (Staphylococcus aureus) bacterial strains in vitro compared to the pure βTCP. P(3HB) coatings, enriched with gentamicin or ceftazidime, exhibited burst and sustained release of the antibiotics from the scaffolds up to 120 h further intensifying the bacteria-killing capability, with evident inhibition zones observed in vitro. Moreover, the composites exhibited apatite-forming ability, suggesting their bioactive potential. In vivo evaluation using Caenorhabditis elegans demonstrated the lack of toxicity of the tested materials. The simultaneous incorporation of the dopants and antibiotic-loaded P(3HB) coatings not only offer a dual antibacterial approach but may also facilitate bone regeneration. However, further in vitro and in vivo investigations are needed to assess their potential in clinical application.
PB  - Elsevier
T2  - Ceramics International
T2  - Ceramics InternationalCeramics International
T1  - Silver and silicon doped βTCP scaffolds with gentamicin or ceftazidime loaded P(3HB) coatings as multifunctional biomaterials for bone regeneration
VL  - n/a
DO  - 10.1016/j.ceramint.2024.05.178
ER  - 

@article{
author = "Skibiński, Szymon and Czechowska, Joanna P. and Milivojević, Dušan and Nikodinović-Runić, Jasmina and Guzik, Maciej and Zima, Aneta",
year = "2024",
abstract = "The risk of bacterial infections is a significant challenge faced frequently in the use of implants or scaffolds for bone regeneration. Therefore, this study focusses on the development and characterisation of novel β tricalcium phosphate (βTCP) scaffolds co-doped with silver and silicon, along with composites coated with antibiotic-loaded poly(3-hydroxybutyrate) (P(3HB)) layers. The successful incorporation of silver and silicon dopants while maintaining the formation of βTCP phase was confirmed using X-ray fluorescence (XRF), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Raman spectroscopy, and scanning electron microscopy/energy-dispersive X-ray spectroscopy (SEM/EDS) analysis. The developed materials demonstrated comparable total and open porosity (∼64–70 vol%), suggesting high interconnectivity between pores conducive to nutrient transport and tissue repair. The increase in compressive strength was achieved for both doped (4.73 ± 0.79 MPa) and P(3HB) coated (5.79 ± 0.92 MPa) scaffolds due to the fine bioceramic microstructure and polymeric coating. Silver and silicon-modified βTCP demonstrated enhanced growth inhibition of Gram-negative (Pseudomonas aeruginosa, Escherichia coli) and Gram-positive (Staphylococcus aureus) bacterial strains in vitro compared to the pure βTCP. P(3HB) coatings, enriched with gentamicin or ceftazidime, exhibited burst and sustained release of the antibiotics from the scaffolds up to 120 h further intensifying the bacteria-killing capability, with evident inhibition zones observed in vitro. Moreover, the composites exhibited apatite-forming ability, suggesting their bioactive potential. In vivo evaluation using Caenorhabditis elegans demonstrated the lack of toxicity of the tested materials. The simultaneous incorporation of the dopants and antibiotic-loaded P(3HB) coatings not only offer a dual antibacterial approach but may also facilitate bone regeneration. However, further in vitro and in vivo investigations are needed to assess their potential in clinical application.",
publisher = "Elsevier",
journal = "Ceramics International, Ceramics InternationalCeramics International",
title = "Silver and silicon doped βTCP scaffolds with gentamicin or ceftazidime loaded P(3HB) coatings as multifunctional biomaterials for bone regeneration",
volume = "n/a",
doi = "10.1016/j.ceramint.2024.05.178"
}

Skibiński, S., Czechowska, J. P., Milivojević, D., Nikodinović-Runić, J., Guzik, M.,& Zima, A.. (2024). Silver and silicon doped βTCP scaffolds with gentamicin or ceftazidime loaded P(3HB) coatings as multifunctional biomaterials for bone regeneration. in Ceramics International
Elsevier., n/a.
https://doi.org/10.1016/j.ceramint.2024.05.178

Skibiński S, Czechowska JP, Milivojević D, Nikodinović-Runić J, Guzik M, Zima A. Silver and silicon doped βTCP scaffolds with gentamicin or ceftazidime loaded P(3HB) coatings as multifunctional biomaterials for bone regeneration. in Ceramics International. 2024;n/a.
doi:10.1016/j.ceramint.2024.05.178 .

Skibiński, Szymon, Czechowska, Joanna P., Milivojević, Dušan, Nikodinović-Runić, Jasmina, Guzik, Maciej, Zima, Aneta, "Silver and silicon doped βTCP scaffolds with gentamicin or ceftazidime loaded P(3HB) coatings as multifunctional biomaterials for bone regeneration" in Ceramics International, n/a (2024),
https://doi.org/10.1016/j.ceramint.2024.05.178 . .

TY  - JOUR
AU  - Pantelić, Brana
AU  - Škaro Bogojević, Sanja
AU  - Milivojević, Dušan
AU  - Ilić-Tomić, Tatjana
AU  - Lončarević, Branka
AU  - Beskoski, Vladimir
AU  - Maslak, Veselin
AU  - Guzik, Maciej
AU  - Makryniotis, Konstantinos
AU  - Taxeidis, George
AU  - Siaperas, Romanos
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.mdpi.com/2073-4344/13/2/278
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1790
AB  - Polyurethanes (PUs) are an exceedingly heterogeneous group of plastic polymers, widely used in a variety of industries from construction to medical implants. In the past decades, we have witnessed the accumulation of PU waste and its detrimental environmental impacts. PUs have been identified as one of the most toxic polymers leaching hazardous compounds derived both from the polymer itself and the additives used in production. Further environmental impact assessment, identification and characterization of substances derived from PU materials and establishing efficient degradation strategies are crucial. Thus, a selection of eight synthetic model compounds which represent partial PU hydrolysis products were synthesized and characterized both in terms of toxicity and suitability to be used as substrates for the identification of novel biocatalysts for PU biodegradation. Overall, the compounds exhibited low in vitro cytotoxicity against a healthy human fibroblast cell line and virtually no toxic effect on the nematode Caenorhabditis elegans up to 500 µg mL−1, and two of the substrates showed moderate aquatic ecotoxicity with EC50 values 53 µg mL−1 and 45 µg mL−1, respectively, on Aliivibrio fischeri. The compounds were successfully applied to study the mechanism of ester and urethane bond cleaving preference of known plastic-degrading enzymes and were used to single out a novel PU-degrading biocatalyst, Amycolatopsis mediterranei ISP5501, among 220 microbial strains. A. mediterranei ISP5501 can also degrade commercially available polyether and polyester PU materials, reducing the average molecular number of the polymer up to 13.5%. This study uncovered a biocatalyst capable of degrading different types of PUs and identified potential enzymes responsible as a key step in developing biotechnological process for PU waste treatment options.
T2  - Catalysts
T2  - Catalysts
T1  - Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts
IS  - 2
SP  - 278
VL  - 13
DO  - 10.3390/catal13020278
ER  - 

@article{
author = "Pantelić, Brana and Škaro Bogojević, Sanja and Milivojević, Dušan and Ilić-Tomić, Tatjana and Lončarević, Branka and Beskoski, Vladimir and Maslak, Veselin and Guzik, Maciej and Makryniotis, Konstantinos and Taxeidis, George and Siaperas, Romanos and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Polyurethanes (PUs) are an exceedingly heterogeneous group of plastic polymers, widely used in a variety of industries from construction to medical implants. In the past decades, we have witnessed the accumulation of PU waste and its detrimental environmental impacts. PUs have been identified as one of the most toxic polymers leaching hazardous compounds derived both from the polymer itself and the additives used in production. Further environmental impact assessment, identification and characterization of substances derived from PU materials and establishing efficient degradation strategies are crucial. Thus, a selection of eight synthetic model compounds which represent partial PU hydrolysis products were synthesized and characterized both in terms of toxicity and suitability to be used as substrates for the identification of novel biocatalysts for PU biodegradation. Overall, the compounds exhibited low in vitro cytotoxicity against a healthy human fibroblast cell line and virtually no toxic effect on the nematode Caenorhabditis elegans up to 500 µg mL−1, and two of the substrates showed moderate aquatic ecotoxicity with EC50 values 53 µg mL−1 and 45 µg mL−1, respectively, on Aliivibrio fischeri. The compounds were successfully applied to study the mechanism of ester and urethane bond cleaving preference of known plastic-degrading enzymes and were used to single out a novel PU-degrading biocatalyst, Amycolatopsis mediterranei ISP5501, among 220 microbial strains. A. mediterranei ISP5501 can also degrade commercially available polyether and polyester PU materials, reducing the average molecular number of the polymer up to 13.5%. This study uncovered a biocatalyst capable of degrading different types of PUs and identified potential enzymes responsible as a key step in developing biotechnological process for PU waste treatment options.",
journal = "Catalysts, Catalysts",
title = "Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts",
number = "2",
pages = "278",
volume = "13",
doi = "10.3390/catal13020278"
}

Pantelić, B., Škaro Bogojević, S., Milivojević, D., Ilić-Tomić, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E.,& Nikodinović-Runić, J.. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. in Catalysts, 13(2), 278.
https://doi.org/10.3390/catal13020278

Pantelić B, Škaro Bogojević S, Milivojević D, Ilić-Tomić T, Lončarević B, Beskoski V, Maslak V, Guzik M, Makryniotis K, Taxeidis G, Siaperas R, Topakas E, Nikodinović-Runić J. Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. in Catalysts. 2023;13(2):278.
doi:10.3390/catal13020278 .

Pantelić, Brana, Škaro Bogojević, Sanja, Milivojević, Dušan, Ilić-Tomić, Tatjana, Lončarević, Branka, Beskoski, Vladimir, Maslak, Veselin, Guzik, Maciej, Makryniotis, Konstantinos, Taxeidis, George, Siaperas, Romanos, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts" in Catalysts, 13, no. 2 (2023):278,
https://doi.org/10.3390/catal13020278 . .

TY  - DATA
AU  - Pantelić, Brana
AU  - Škaro Bogojević, Sanja
AU  - Milivojević, Dušan
AU  - Ilić-Tomić, Tatjana
AU  - Lončarević, Branka
AU  - Beskoski, Vladimir
AU  - Maslak, Veselin
AU  - Guzik, Maciej
AU  - Makryniotis, Konstantinos
AU  - Taxeidis, George
AU  - Siaperas, Romanos
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.mdpi.com/2073-4344/13/2/278
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1792
T2  - Catalysts
T1  - Supporting information: Pantelic, B., Skaro Bogojevic, S., Milivojevic, D., Ilic-Tomic, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E., & Nikodinovic-Runic, J. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. Catalysts, 13(2), Art. 2. https://doi.org/10.3390/catal13020278
IS  - 2
SP  - 278
VL  - 13
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1792
ER  - 

@misc{
author = "Pantelić, Brana and Škaro Bogojević, Sanja and Milivojević, Dušan and Ilić-Tomić, Tatjana and Lončarević, Branka and Beskoski, Vladimir and Maslak, Veselin and Guzik, Maciej and Makryniotis, Konstantinos and Taxeidis, George and Siaperas, Romanos and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2023",
journal = "Catalysts",
title = "Supporting information: Pantelic, B., Skaro Bogojevic, S., Milivojevic, D., Ilic-Tomic, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E., & Nikodinovic-Runic, J. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. Catalysts, 13(2), Art. 2. https://doi.org/10.3390/catal13020278",
number = "2",
pages = "278",
volume = "13",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1792"
}

Pantelić, B., Škaro Bogojević, S., Milivojević, D., Ilić-Tomić, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E.,& Nikodinović-Runić, J.. (2023). Supporting information: Pantelic, B., Skaro Bogojevic, S., Milivojevic, D., Ilic-Tomic, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E., & Nikodinovic-Runic, J. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. Catalysts, 13(2), Art. 2. https://doi.org/10.3390/catal13020278. in Catalysts, 13(2), 278.
https://hdl.handle.net/21.15107/rcub_imagine_1792

Pantelić B, Škaro Bogojević S, Milivojević D, Ilić-Tomić T, Lončarević B, Beskoski V, Maslak V, Guzik M, Makryniotis K, Taxeidis G, Siaperas R, Topakas E, Nikodinović-Runić J. Supporting information: Pantelic, B., Skaro Bogojevic, S., Milivojevic, D., Ilic-Tomic, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E., & Nikodinovic-Runic, J. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. Catalysts, 13(2), Art. 2. https://doi.org/10.3390/catal13020278. in Catalysts. 2023;13(2):278.
https://hdl.handle.net/21.15107/rcub_imagine_1792 .

Pantelić, Brana, Škaro Bogojević, Sanja, Milivojević, Dušan, Ilić-Tomić, Tatjana, Lončarević, Branka, Beskoski, Vladimir, Maslak, Veselin, Guzik, Maciej, Makryniotis, Konstantinos, Taxeidis, George, Siaperas, Romanos, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Supporting information: Pantelic, B., Skaro Bogojevic, S., Milivojevic, D., Ilic-Tomic, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E., & Nikodinovic-Runic, J. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. Catalysts, 13(2), Art. 2. https://doi.org/10.3390/catal13020278" in Catalysts, 13, no. 2 (2023):278,
https://hdl.handle.net/21.15107/rcub_imagine_1792 .

TY  - DATA
AU  - Solarz, Daria
AU  - Witko, Tomasz
AU  - Karcz, Robert
AU  - Malagurski, Ivana
AU  - Ponjavić, Marijana
AU  - Levic, Steva
AU  - Nešić, Aleksandra
AU  - Guzik, Maciej
AU  - Savić, Sanja
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://pubs.rsc.org/en/content/articlelanding/2023/ra/d3ra03021k
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2060
AB  - Polyhydroxyoctanoate, as a biocompatible and biodegradable biopolymer, represents an ideal candidate for biomedical applications. However, physical properties make it unsuitable for electrospinning, currently the most widely used technique for fabrication of fibrous scaffolds. To overcome this, it was blended with polylactic acid and polymer blend fibrous biomaterials were produced by electrospinning. The obtained PLA/PHO fibers were cylindrical, smaller in size, more hydrophilic and had a higher degree of biopolymer crystallinity and more favorable mechanical properties in comparison to the pure PLA sample. Cytotoxicity evaluation with human lung fibroblasts (MRC5 cells) combined with confocal microscopy were used to visualize mouse embryonic fibroblasts (MEF 3T3 cell line) migration and distribution showed that PLA/PHO samples support exceptional cell adhesion and viability, indicating excellent biocompatibility. The obtained results suggest that PLA/PHO fibrous biomaterials can be potentially used as biocompatible, biomimetic scaffolds for tissue engineering applications.
T2  - RSC Advances
T1  - Supplementary data for article: Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavić, M., Levic, S., Nešić, A., Guzik, M., Savić, S.,& Nikodinović-Runić, J.. (2023). Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances, 13(34), 24112-24128. https://doi.org/10.1039/D3RA03021K
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2060
ER  - 

@misc{
author = "Solarz, Daria and Witko, Tomasz and Karcz, Robert and Malagurski, Ivana and Ponjavić, Marijana and Levic, Steva and Nešić, Aleksandra and Guzik, Maciej and Savić, Sanja and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Polyhydroxyoctanoate, as a biocompatible and biodegradable biopolymer, represents an ideal candidate for biomedical applications. However, physical properties make it unsuitable for electrospinning, currently the most widely used technique for fabrication of fibrous scaffolds. To overcome this, it was blended with polylactic acid and polymer blend fibrous biomaterials were produced by electrospinning. The obtained PLA/PHO fibers were cylindrical, smaller in size, more hydrophilic and had a higher degree of biopolymer crystallinity and more favorable mechanical properties in comparison to the pure PLA sample. Cytotoxicity evaluation with human lung fibroblasts (MRC5 cells) combined with confocal microscopy were used to visualize mouse embryonic fibroblasts (MEF 3T3 cell line) migration and distribution showed that PLA/PHO samples support exceptional cell adhesion and viability, indicating excellent biocompatibility. The obtained results suggest that PLA/PHO fibrous biomaterials can be potentially used as biocompatible, biomimetic scaffolds for tissue engineering applications.",
journal = "RSC Advances",
title = "Supplementary data for article: Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavić, M., Levic, S., Nešić, A., Guzik, M., Savić, S.,& Nikodinović-Runić, J.. (2023). Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances, 13(34), 24112-24128. https://doi.org/10.1039/D3RA03021K",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2060"
}

Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavić, M., Levic, S., Nešić, A., Guzik, M., Savić, S.,& Nikodinović-Runić, J.. (2023). Supplementary data for article: Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavić, M., Levic, S., Nešić, A., Guzik, M., Savić, S.,& Nikodinović-Runić, J.. (2023). Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances, 13(34), 24112-24128. https://doi.org/10.1039/D3RA03021K. in RSC Advances.
https://hdl.handle.net/21.15107/rcub_imagine_2060

Solarz D, Witko T, Karcz R, Malagurski I, Ponjavić M, Levic S, Nešić A, Guzik M, Savić S, Nikodinović-Runić J. Supplementary data for article: Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavić, M., Levic, S., Nešić, A., Guzik, M., Savić, S.,& Nikodinović-Runić, J.. (2023). Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances, 13(34), 24112-24128. https://doi.org/10.1039/D3RA03021K. in RSC Advances. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2060 .

Solarz, Daria, Witko, Tomasz, Karcz, Robert, Malagurski, Ivana, Ponjavić, Marijana, Levic, Steva, Nešić, Aleksandra, Guzik, Maciej, Savić, Sanja, Nikodinović-Runić, Jasmina, "Supplementary data for article: Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavić, M., Levic, S., Nešić, A., Guzik, M., Savić, S.,& Nikodinović-Runić, J.. (2023). Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances, 13(34), 24112-24128. https://doi.org/10.1039/D3RA03021K" in RSC Advances (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2060 .

TY  - JOUR
AU  - Solarz, Daria
AU  - Witko, Tomasz
AU  - Karcz, Robert
AU  - Malagurski, Ivana
AU  - Ponjavić, Marijana
AU  - Levic, Steva
AU  - Nešić, Aleksandra
AU  - Guzik, Maciej
AU  - Savić, Sanja
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://pubs.rsc.org/en/content/articlelanding/2023/ra/d3ra03021k
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2059
AB  - Polyhydroxyoctanoate, as a biocompatible and biodegradable biopolymer, represents an ideal candidate for biomedical applications. However, physical properties make it unsuitable for electrospinning, currently the most widely used technique for fabrication of fibrous scaffolds. To overcome this, it was blended with polylactic acid and polymer blend fibrous biomaterials were produced by electrospinning. The obtained PLA/PHO fibers were cylindrical, smaller in size, more hydrophilic and had a higher degree of biopolymer crystallinity and more favorable mechanical properties in comparison to the pure PLA sample. Cytotoxicity evaluation with human lung fibroblasts (MRC5 cells) combined with confocal microscopy were used to visualize mouse embryonic fibroblasts (MEF 3T3 cell line) migration and distribution showed that PLA/PHO samples support exceptional cell adhesion and viability, indicating excellent biocompatibility. The obtained results suggest that PLA/PHO fibrous biomaterials can be potentially used as biocompatible, biomimetic scaffolds for tissue engineering applications.
T2  - RSC Advances
T1  - Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications
EP  - 24128
IS  - 34
SP  - 24112
VL  - 13
DO  - 10.1039/D3RA03021K
ER  - 

@article{
author = "Solarz, Daria and Witko, Tomasz and Karcz, Robert and Malagurski, Ivana and Ponjavić, Marijana and Levic, Steva and Nešić, Aleksandra and Guzik, Maciej and Savić, Sanja and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Polyhydroxyoctanoate, as a biocompatible and biodegradable biopolymer, represents an ideal candidate for biomedical applications. However, physical properties make it unsuitable for electrospinning, currently the most widely used technique for fabrication of fibrous scaffolds. To overcome this, it was blended with polylactic acid and polymer blend fibrous biomaterials were produced by electrospinning. The obtained PLA/PHO fibers were cylindrical, smaller in size, more hydrophilic and had a higher degree of biopolymer crystallinity and more favorable mechanical properties in comparison to the pure PLA sample. Cytotoxicity evaluation with human lung fibroblasts (MRC5 cells) combined with confocal microscopy were used to visualize mouse embryonic fibroblasts (MEF 3T3 cell line) migration and distribution showed that PLA/PHO samples support exceptional cell adhesion and viability, indicating excellent biocompatibility. The obtained results suggest that PLA/PHO fibrous biomaterials can be potentially used as biocompatible, biomimetic scaffolds for tissue engineering applications.",
journal = "RSC Advances",
title = "Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications",
pages = "24128-24112",
number = "34",
volume = "13",
doi = "10.1039/D3RA03021K"
}

Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavić, M., Levic, S., Nešić, A., Guzik, M., Savić, S.,& Nikodinović-Runić, J.. (2023). Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances, 13(34), 24112-24128.
https://doi.org/10.1039/D3RA03021K

Solarz D, Witko T, Karcz R, Malagurski I, Ponjavić M, Levic S, Nešić A, Guzik M, Savić S, Nikodinović-Runić J. Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances. 2023;13(34):24112-24128.
doi:10.1039/D3RA03021K .

Solarz, Daria, Witko, Tomasz, Karcz, Robert, Malagurski, Ivana, Ponjavić, Marijana, Levic, Steva, Nešić, Aleksandra, Guzik, Maciej, Savić, Sanja, Nikodinović-Runić, Jasmina, "Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications" in RSC Advances, 13, no. 34 (2023):24112-24128,
https://doi.org/10.1039/D3RA03021K . .

TY  - DATA
AU  - Snoch, Wojciech
AU  - Jarek, Ewelina
AU  - Milivojević, Dušan
AU  - Nikodinović-Runić, Jasmina
AU  - Guzik, Maciej
PY  - 2023
UR  - https://www.frontiersin.org/articles/10.3389/fbioe.2023.1112053
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1781
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Supplementary information for the article: Snoch, W., Jarek, E., Milivojevic, D., Nikodinovic-Runic, J., & Guzik, M. (2023). Physicochemical studies of novel sugar fatty acid esters based on (R)-3-hydroxylated acids derived from bacterial polyhydroxyalkanoates and their potential environmental impact. Frontiers in Bioengineering and Biotechnology, 11. https://www.frontiersin.org/articles/10.3389/fbioe.2023.1112053
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1781
ER  - 

@misc{
author = "Snoch, Wojciech and Jarek, Ewelina and Milivojević, Dušan and Nikodinović-Runić, Jasmina and Guzik, Maciej",
year = "2023",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Supplementary information for the article: Snoch, W., Jarek, E., Milivojevic, D., Nikodinovic-Runic, J., & Guzik, M. (2023). Physicochemical studies of novel sugar fatty acid esters based on (R)-3-hydroxylated acids derived from bacterial polyhydroxyalkanoates and their potential environmental impact. Frontiers in Bioengineering and Biotechnology, 11. https://www.frontiersin.org/articles/10.3389/fbioe.2023.1112053",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1781"
}

Snoch, W., Jarek, E., Milivojević, D., Nikodinović-Runić, J.,& Guzik, M.. (2023). Supplementary information for the article: Snoch, W., Jarek, E., Milivojevic, D., Nikodinovic-Runic, J., & Guzik, M. (2023). Physicochemical studies of novel sugar fatty acid esters based on (R)-3-hydroxylated acids derived from bacterial polyhydroxyalkanoates and their potential environmental impact. Frontiers in Bioengineering and Biotechnology, 11. https://www.frontiersin.org/articles/10.3389/fbioe.2023.1112053. in Frontiers in Bioengineering and Biotechnology.
https://hdl.handle.net/21.15107/rcub_imagine_1781

Snoch W, Jarek E, Milivojević D, Nikodinović-Runić J, Guzik M. Supplementary information for the article: Snoch, W., Jarek, E., Milivojevic, D., Nikodinovic-Runic, J., & Guzik, M. (2023). Physicochemical studies of novel sugar fatty acid esters based on (R)-3-hydroxylated acids derived from bacterial polyhydroxyalkanoates and their potential environmental impact. Frontiers in Bioengineering and Biotechnology, 11. https://www.frontiersin.org/articles/10.3389/fbioe.2023.1112053. in Frontiers in Bioengineering and Biotechnology. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_1781 .

Snoch, Wojciech, Jarek, Ewelina, Milivojević, Dušan, Nikodinović-Runić, Jasmina, Guzik, Maciej, "Supplementary information for the article: Snoch, W., Jarek, E., Milivojevic, D., Nikodinovic-Runic, J., & Guzik, M. (2023). Physicochemical studies of novel sugar fatty acid esters based on (R)-3-hydroxylated acids derived from bacterial polyhydroxyalkanoates and their potential environmental impact. Frontiers in Bioengineering and Biotechnology, 11. https://www.frontiersin.org/articles/10.3389/fbioe.2023.1112053" in Frontiers in Bioengineering and Biotechnology (2023),
https://hdl.handle.net/21.15107/rcub_imagine_1781 .

TY  - JOUR
AU  - Snoch, Wojciech
AU  - Jarek, Ewelina
AU  - Milivojević, Dušan
AU  - Nikodinović-Runić, Jasmina
AU  - Guzik, Maciej
PY  - 2023
UR  - https://www.frontiersin.org/articles/10.3389/fbioe.2023.1112053
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1780
AB  - Sugar fatty acids esters are popular compounds widely used in both the nutritional, cosmetic and pharmaceutical industries due to their amphiphilic structure and consequent ability to reduce the surface tension of solutions. Furthermore, an important aspect in the implementation of any additives and formulations is their environmental impact. The properties of the esters depend on the type of sugar used and the hydrophobic component. In this work, selected physicochemical properties of new sugar esters based on lactose, glucose and galactose and hydroxy acids derived from bacterial polyhydroxyalkanoates are shown for the first time. Values for critical aggregation concentration, surface activity and pH make it possible that these esters could compete with other commercially used esters of similar chemical structure. The investigated compounds showed moderate emulsion stabilization abilities presented on the example of water-oil systems containing squalene and body oil. Their potential environmental impact appears to be low, as the esters are not toxic to Caenorhabditis elegans even at concentrations much higher than the critical aggregation concentration.
T2  - Frontiers in Bioengineering and Biotechnology
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Physicochemical studies of novel sugar fatty acid esters based on (R)-3-hydroxylated acids derived from bacterial polyhydroxyalkanoates and their potential environmental impact
VL  - 11
DO  - 10.3389/fbioe.2023.1112053
ER  - 

@article{
author = "Snoch, Wojciech and Jarek, Ewelina and Milivojević, Dušan and Nikodinović-Runić, Jasmina and Guzik, Maciej",
year = "2023",
abstract = "Sugar fatty acids esters are popular compounds widely used in both the nutritional, cosmetic and pharmaceutical industries due to their amphiphilic structure and consequent ability to reduce the surface tension of solutions. Furthermore, an important aspect in the implementation of any additives and formulations is their environmental impact. The properties of the esters depend on the type of sugar used and the hydrophobic component. In this work, selected physicochemical properties of new sugar esters based on lactose, glucose and galactose and hydroxy acids derived from bacterial polyhydroxyalkanoates are shown for the first time. Values for critical aggregation concentration, surface activity and pH make it possible that these esters could compete with other commercially used esters of similar chemical structure. The investigated compounds showed moderate emulsion stabilization abilities presented on the example of water-oil systems containing squalene and body oil. Their potential environmental impact appears to be low, as the esters are not toxic to Caenorhabditis elegans even at concentrations much higher than the critical aggregation concentration.",
journal = "Frontiers in Bioengineering and Biotechnology, Frontiers in Bioengineering and Biotechnology",
title = "Physicochemical studies of novel sugar fatty acid esters based on (R)-3-hydroxylated acids derived from bacterial polyhydroxyalkanoates and their potential environmental impact",
volume = "11",
doi = "10.3389/fbioe.2023.1112053"
}

Snoch, W., Jarek, E., Milivojević, D., Nikodinović-Runić, J.,& Guzik, M.. (2023). Physicochemical studies of novel sugar fatty acid esters based on (R)-3-hydroxylated acids derived from bacterial polyhydroxyalkanoates and their potential environmental impact. in Frontiers in Bioengineering and Biotechnology, 11.
https://doi.org/10.3389/fbioe.2023.1112053

Snoch W, Jarek E, Milivojević D, Nikodinović-Runić J, Guzik M. Physicochemical studies of novel sugar fatty acid esters based on (R)-3-hydroxylated acids derived from bacterial polyhydroxyalkanoates and their potential environmental impact. in Frontiers in Bioengineering and Biotechnology. 2023;11.
doi:10.3389/fbioe.2023.1112053 .

Snoch, Wojciech, Jarek, Ewelina, Milivojević, Dušan, Nikodinović-Runić, Jasmina, Guzik, Maciej, "Physicochemical studies of novel sugar fatty acid esters based on (R)-3-hydroxylated acids derived from bacterial polyhydroxyalkanoates and their potential environmental impact" in Frontiers in Bioengineering and Biotechnology, 11 (2023),
https://doi.org/10.3389/fbioe.2023.1112053 . .

TY  - JOUR
AU  - Pekmezović, Marina
AU  - Krusić, Melina Kalagasidis
AU  - Malagurski, Ivana
AU  - Milovanović, Jelena
AU  - Stepien, Karolina
AU  - Guzik, Maciej
AU  - Charifou, Romina
AU  - Babu, Ramesh
AU  - O'Connor, Kevin
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1440
AB  - Novel biodegradable and biocompatible formulations of "old" but "gold" drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (similar to 50 mu m). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.
PB  - MDPI, Basel
T2  - Antibiotics-Basel
T1  - Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency
IS  - 6
VL  - 10
DO  - 10.3390/antibiotics10060737
ER  - 

@article{
author = "Pekmezović, Marina and Krusić, Melina Kalagasidis and Malagurski, Ivana and Milovanović, Jelena and Stepien, Karolina and Guzik, Maciej and Charifou, Romina and Babu, Ramesh and O'Connor, Kevin and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "Novel biodegradable and biocompatible formulations of "old" but "gold" drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (similar to 50 mu m). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.",
publisher = "MDPI, Basel",
journal = "Antibiotics-Basel",
title = "Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency",
number = "6",
volume = "10",
doi = "10.3390/antibiotics10060737"
}

Pekmezović, M., Krusić, M. K., Malagurski, I., Milovanović, J., Stepien, K., Guzik, M., Charifou, R., Babu, R., O'Connor, K.,& Nikodinović-Runić, J.. (2021). Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency. in Antibiotics-Basel
MDPI, Basel., 10(6).
https://doi.org/10.3390/antibiotics10060737

Pekmezović M, Krusić MK, Malagurski I, Milovanović J, Stepien K, Guzik M, Charifou R, Babu R, O'Connor K, Nikodinović-Runić J. Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency. in Antibiotics-Basel. 2021;10(6).
doi:10.3390/antibiotics10060737 .

Pekmezović, Marina, Krusić, Melina Kalagasidis, Malagurski, Ivana, Milovanović, Jelena, Stepien, Karolina, Guzik, Maciej, Charifou, Romina, Babu, Ramesh, O'Connor, Kevin, Nikodinović-Runić, Jasmina, "Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency" in Antibiotics-Basel, 10, no. 6 (2021),
https://doi.org/10.3390/antibiotics10060737 . .

TY  - JOUR
AU  - Milovanović, Jelena
AU  - Škaro Bogojević, Sanja
AU  - Šenerović, Lidija
AU  - Vasiljević, Branka
AU  - Guzik, Maciej
AU  - Kenny, Shane T.
AU  - Maslak, Veselin
AU  - Nikodinović-Runić, Jasmina
AU  - O'Connor, Kevin 
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/974
AB  - A library of 18 different compounds was synthesized starting from (R)-3-hydroxyoctanoic acid which is derived from the bacterial polymer polyhydroxyalkanoate (PHA). Ten derivatives, including halo and unsaturated methyl and benzyl esters, were synthesized and characterized for the first time. Given that (R)-3-hydroxyalkanoic acids are known to have biological activity, the new compounds were evaluated for antimicrobial activity and in vitro antiproliferative effect with mammalian cell lines. The presence of the carboxylic group was essential for the antimicrobial activity, with minimal inhibitory concentrations against a panel of bacteria (Gram-positive and Gram-negative) and fungi (Candida albicans and Microsporum gypseum) in the range 2.8-7.0 mM and 0.1-6.3 mM, respectively. 3-Halogenated octanoic acids exhibited the ability to inhibit C. albicans hyphae formation. In addition, (R)-3-hydroxyoctanoic and (E)-oct-2-enoic acids inhibited quorum sensing-regulated pyocyanin production in the opportunistic pathogen Pseudomonas aeruginosa PAO1. Generally, derivatives did not inhibit mammalian cell proliferation even at 3-mM concentrations, while only (E)-oct-2-enoic and 3-oxooctanoic acid had IC50 values of 1.7 and 1.6 mM with the human lung fibroblast cell line.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds
EP  - 172
IS  - 1
SP  - 161
VL  - 100
DO  - 10.1007/s00253-015-6984-4
ER  - 

@article{
author = "Milovanović, Jelena and Škaro Bogojević, Sanja and Šenerović, Lidija and Vasiljević, Branka and Guzik, Maciej and Kenny, Shane T. and Maslak, Veselin and Nikodinović-Runić, Jasmina and O'Connor, Kevin ",
year = "2016",
abstract = "A library of 18 different compounds was synthesized starting from (R)-3-hydroxyoctanoic acid which is derived from the bacterial polymer polyhydroxyalkanoate (PHA). Ten derivatives, including halo and unsaturated methyl and benzyl esters, were synthesized and characterized for the first time. Given that (R)-3-hydroxyalkanoic acids are known to have biological activity, the new compounds were evaluated for antimicrobial activity and in vitro antiproliferative effect with mammalian cell lines. The presence of the carboxylic group was essential for the antimicrobial activity, with minimal inhibitory concentrations against a panel of bacteria (Gram-positive and Gram-negative) and fungi (Candida albicans and Microsporum gypseum) in the range 2.8-7.0 mM and 0.1-6.3 mM, respectively. 3-Halogenated octanoic acids exhibited the ability to inhibit C. albicans hyphae formation. In addition, (R)-3-hydroxyoctanoic and (E)-oct-2-enoic acids inhibited quorum sensing-regulated pyocyanin production in the opportunistic pathogen Pseudomonas aeruginosa PAO1. Generally, derivatives did not inhibit mammalian cell proliferation even at 3-mM concentrations, while only (E)-oct-2-enoic and 3-oxooctanoic acid had IC50 values of 1.7 and 1.6 mM with the human lung fibroblast cell line.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds",
pages = "172-161",
number = "1",
volume = "100",
doi = "10.1007/s00253-015-6984-4"
}

Milovanović, J., Škaro Bogojević, S., Šenerović, L., Vasiljević, B., Guzik, M., Kenny, S. T., Maslak, V., Nikodinović-Runić, J.,& O'Connor, K.. (2016). Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds. in Applied Microbiology and Biotechnology
Springer, New York., 100(1), 161-172.
https://doi.org/10.1007/s00253-015-6984-4

Milovanović J, Škaro Bogojević S, Šenerović L, Vasiljević B, Guzik M, Kenny ST, Maslak V, Nikodinović-Runić J, O'Connor K. Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds. in Applied Microbiology and Biotechnology. 2016;100(1):161-172.
doi:10.1007/s00253-015-6984-4 .

Milovanović, Jelena, Škaro Bogojević, Sanja, Šenerović, Lidija, Vasiljević, Branka, Guzik, Maciej, Kenny, Shane T., Maslak, Veselin, Nikodinović-Runić, Jasmina, O'Connor, Kevin , "Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds" in Applied Microbiology and Biotechnology, 100, no. 1 (2016):161-172,
https://doi.org/10.1007/s00253-015-6984-4 . .

TY  - JOUR
AU  - Szwej, Emilia
AU  - Devocelle, Marc
AU  - Kenny, Shane
AU  - Guzik, Maciej
AU  - O'Connor, Stephen
AU  - Nikodinović-Runić, Jasmina
AU  - Milovanović, Jelena
AU  - Maslak, Veselin
AU  - Byrne, Annete T.
AU  - Gallagher, William M.
AU  - Zulian, Qun Ren
AU  - Zinn, Manfred
AU  - O'Connor, Kevin 
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/877
AB  - Conjugation of DP18L peptide with (R)-3-hydroxydecanoic acid, derived from the biopolymer polyhydroxyalkanoate, enhances its anti-cancer activity (O'Connor et al., 2013. Biomaterials 34, 2710-2718). However, it is unknown if other (R)-3-hydroxyalkanoic acids (R3HA5) can enhance peptide activity, if chain length affects enhancement, and what effect R3HA5 have on peptide structure. Here we show that the degree of enhancement of peptide (DP18L) anti-cancer activity by R3HA5 is carbon chain length dependent. In all but one example the R3HA conjugated peptides were more active against cancer cells than the unconjugated peptides. However, R3HA5 with 9 and 10 carbons were most effective at improving DPI 8L activity. DPI 8L peptide variant DPI 7L, missing a hydrophobic amino acid (leucine residue 4) exhibited lower efficacy against MiaPaCa cells. Circular dichroism analysis showed DP17L had a lower alpha helix content and the conjugation of any R3HA ((R)-3-hydroxyhexanoic acid to (R)-3-hydroxydodecanoic acid) to DPI 7L returned the helix content back to levels of DPI 8L. However (R)-3-hydroxyhexanoic did not enhance the anti-cancer activity of DPI 7L and at least 7 carbons were needed in the R3HA to enhance activity of D17L. DP17L needs a longer chain R3HA to achieve the same activity as DP18L conjugated to an R3HA. As a first step to assess the synthetic potential of polyhydroxyalkanoate derived R3HA5, (R)-3-hydroxydecanoic acid was synthetically converted to (+/-)3-chlorodecanoic acid, which when conjugated to DP18L improved its antiproliferative activity against MiaPaCa cells.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Biotechnology
T1  - The chain length of biologically produced (R)-3-hydroxyalkanoic acid affects biological activity and structure of anti-cancer peptides
EP  - 12
SP  - 7
VL  - 204
DO  - 10.1016/j.jbiotec.2015.02.036
ER  - 

@article{
author = "Szwej, Emilia and Devocelle, Marc and Kenny, Shane and Guzik, Maciej and O'Connor, Stephen and Nikodinović-Runić, Jasmina and Milovanović, Jelena and Maslak, Veselin and Byrne, Annete T. and Gallagher, William M. and Zulian, Qun Ren and Zinn, Manfred and O'Connor, Kevin ",
year = "2015",
abstract = "Conjugation of DP18L peptide with (R)-3-hydroxydecanoic acid, derived from the biopolymer polyhydroxyalkanoate, enhances its anti-cancer activity (O'Connor et al., 2013. Biomaterials 34, 2710-2718). However, it is unknown if other (R)-3-hydroxyalkanoic acids (R3HA5) can enhance peptide activity, if chain length affects enhancement, and what effect R3HA5 have on peptide structure. Here we show that the degree of enhancement of peptide (DP18L) anti-cancer activity by R3HA5 is carbon chain length dependent. In all but one example the R3HA conjugated peptides were more active against cancer cells than the unconjugated peptides. However, R3HA5 with 9 and 10 carbons were most effective at improving DPI 8L activity. DPI 8L peptide variant DPI 7L, missing a hydrophobic amino acid (leucine residue 4) exhibited lower efficacy against MiaPaCa cells. Circular dichroism analysis showed DP17L had a lower alpha helix content and the conjugation of any R3HA ((R)-3-hydroxyhexanoic acid to (R)-3-hydroxydodecanoic acid) to DPI 7L returned the helix content back to levels of DPI 8L. However (R)-3-hydroxyhexanoic did not enhance the anti-cancer activity of DPI 7L and at least 7 carbons were needed in the R3HA to enhance activity of D17L. DP17L needs a longer chain R3HA to achieve the same activity as DP18L conjugated to an R3HA. As a first step to assess the synthetic potential of polyhydroxyalkanoate derived R3HA5, (R)-3-hydroxydecanoic acid was synthetically converted to (+/-)3-chlorodecanoic acid, which when conjugated to DP18L improved its antiproliferative activity against MiaPaCa cells.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Biotechnology",
title = "The chain length of biologically produced (R)-3-hydroxyalkanoic acid affects biological activity and structure of anti-cancer peptides",
pages = "12-7",
volume = "204",
doi = "10.1016/j.jbiotec.2015.02.036"
}

Szwej, E., Devocelle, M., Kenny, S., Guzik, M., O'Connor, S., Nikodinović-Runić, J., Milovanović, J., Maslak, V., Byrne, A. T., Gallagher, W. M., Zulian, Q. R., Zinn, M.,& O'Connor, K.. (2015). The chain length of biologically produced (R)-3-hydroxyalkanoic acid affects biological activity and structure of anti-cancer peptides. in Journal of Biotechnology
Elsevier Science Bv, Amsterdam., 204, 7-12.
https://doi.org/10.1016/j.jbiotec.2015.02.036

Szwej E, Devocelle M, Kenny S, Guzik M, O'Connor S, Nikodinović-Runić J, Milovanović J, Maslak V, Byrne AT, Gallagher WM, Zulian QR, Zinn M, O'Connor K. The chain length of biologically produced (R)-3-hydroxyalkanoic acid affects biological activity and structure of anti-cancer peptides. in Journal of Biotechnology. 2015;204:7-12.
doi:10.1016/j.jbiotec.2015.02.036 .

Szwej, Emilia, Devocelle, Marc, Kenny, Shane, Guzik, Maciej, O'Connor, Stephen, Nikodinović-Runić, Jasmina, Milovanović, Jelena, Maslak, Veselin, Byrne, Annete T., Gallagher, William M., Zulian, Qun Ren, Zinn, Manfred, O'Connor, Kevin , "The chain length of biologically produced (R)-3-hydroxyalkanoic acid affects biological activity and structure of anti-cancer peptides" in Journal of Biotechnology, 204 (2015):7-12,
https://doi.org/10.1016/j.jbiotec.2015.02.036 . .

TY  - JOUR
AU  - Guzik, Maciej W.
AU  - Narancić, Tanja
AU  - Ilić-Tomić, Tatjana
AU  - Vojnović, Sandra
AU  - Kenny, Shane T.
AU  - Casey, William T.
AU  - Duane, Gearoid F.
AU  - Casey, Eoin
AU  - Woods, Trevor
AU  - Babu, Ramesh
AU  - Nikodinović-Runić, Jasmina
AU  - O'Connor, Kevin 
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/749
AB  - Diverse and elaborate pathways for nutrient utilization, as well as mechanisms to combat unfavourable nutrient conditions make Pseudomonas putida KT2440 a versatile micro-organism able to occupy a range of ecological niches. The fatty acid degradation pathway of P. putida is complex and correlated with biopolymer medium chain length polyhydroxyalkanoate (mcl-PHA) biosynthesis. Little is known about the second step of fatty acid degradation (beta-oxidation) in this strain. In silico analysis of its genome sequence revealed 21 putative acyl-CoA dehydrogenases (ACADs), four of which were functionally characterized through mutagenesis studies. Four mutants with insertionally inactivated ACADs (PP_1893, PP_2039, PP_2048 and PP_2437) grew and accumulated mcl-PHA on a range of fatty acids as the sole source of carbon and energy. Their ability to grow and accumulate biopolymer was differentially negatively affected on various fatty acids, in comparison to the wild-type strain. Inactive PP_2437 exhibited a pattern of reduced growth and PHA accumulation when fatty acids with lengths of 10 to 14 carbon chains were used as substrates. Recombinant expression and biochemical characterization of the purified protein allowed functional annotation in P. putida KT2440 as an ACAD showing clear preference for dodecanoyl-CoA ester as a substrate and optimum activity at 30 degrees C and pH 6.5-7.
PB  - Microbiology Soc, London
T2  - Microbiology-Sgm
T1  - Identification and characterization of an acyl-CoA dehydrogenase from Pseudomonas putida KT2440 that shows preference towards medium to long chain length fatty acids
EP  - 1771
SP  - 1760
VL  - 160
DO  - 10.1099/mic.0.078758-0
ER  - 

@article{
author = "Guzik, Maciej W. and Narancić, Tanja and Ilić-Tomić, Tatjana and Vojnović, Sandra and Kenny, Shane T. and Casey, William T. and Duane, Gearoid F. and Casey, Eoin and Woods, Trevor and Babu, Ramesh and Nikodinović-Runić, Jasmina and O'Connor, Kevin ",
year = "2014",
abstract = "Diverse and elaborate pathways for nutrient utilization, as well as mechanisms to combat unfavourable nutrient conditions make Pseudomonas putida KT2440 a versatile micro-organism able to occupy a range of ecological niches. The fatty acid degradation pathway of P. putida is complex and correlated with biopolymer medium chain length polyhydroxyalkanoate (mcl-PHA) biosynthesis. Little is known about the second step of fatty acid degradation (beta-oxidation) in this strain. In silico analysis of its genome sequence revealed 21 putative acyl-CoA dehydrogenases (ACADs), four of which were functionally characterized through mutagenesis studies. Four mutants with insertionally inactivated ACADs (PP_1893, PP_2039, PP_2048 and PP_2437) grew and accumulated mcl-PHA on a range of fatty acids as the sole source of carbon and energy. Their ability to grow and accumulate biopolymer was differentially negatively affected on various fatty acids, in comparison to the wild-type strain. Inactive PP_2437 exhibited a pattern of reduced growth and PHA accumulation when fatty acids with lengths of 10 to 14 carbon chains were used as substrates. Recombinant expression and biochemical characterization of the purified protein allowed functional annotation in P. putida KT2440 as an ACAD showing clear preference for dodecanoyl-CoA ester as a substrate and optimum activity at 30 degrees C and pH 6.5-7.",
publisher = "Microbiology Soc, London",
journal = "Microbiology-Sgm",
title = "Identification and characterization of an acyl-CoA dehydrogenase from Pseudomonas putida KT2440 that shows preference towards medium to long chain length fatty acids",
pages = "1771-1760",
volume = "160",
doi = "10.1099/mic.0.078758-0"
}

Guzik, M. W., Narancić, T., Ilić-Tomić, T., Vojnović, S., Kenny, S. T., Casey, W. T., Duane, G. F., Casey, E., Woods, T., Babu, R., Nikodinović-Runić, J.,& O'Connor, K.. (2014). Identification and characterization of an acyl-CoA dehydrogenase from Pseudomonas putida KT2440 that shows preference towards medium to long chain length fatty acids. in Microbiology-Sgm
Microbiology Soc, London., 160, 1760-1771.
https://doi.org/10.1099/mic.0.078758-0

Guzik MW, Narancić T, Ilić-Tomić T, Vojnović S, Kenny ST, Casey WT, Duane GF, Casey E, Woods T, Babu R, Nikodinović-Runić J, O'Connor K. Identification and characterization of an acyl-CoA dehydrogenase from Pseudomonas putida KT2440 that shows preference towards medium to long chain length fatty acids. in Microbiology-Sgm. 2014;160:1760-1771.
doi:10.1099/mic.0.078758-0 .

Guzik, Maciej W., Narancić, Tanja, Ilić-Tomić, Tatjana, Vojnović, Sandra, Kenny, Shane T., Casey, William T., Duane, Gearoid F., Casey, Eoin, Woods, Trevor, Babu, Ramesh, Nikodinović-Runić, Jasmina, O'Connor, Kevin , "Identification and characterization of an acyl-CoA dehydrogenase from Pseudomonas putida KT2440 that shows preference towards medium to long chain length fatty acids" in Microbiology-Sgm, 160 (2014):1760-1771,
https://doi.org/10.1099/mic.0.078758-0 . .

TY  - JOUR
AU  - Guzik, Maciej W.
AU  - Kenny, Shane T.
AU  - Duane, Gearoid F.
AU  - Casey, Eoin
AU  - Woods, Trevor
AU  - Babu, Ramesh
AU  - Nikodinović-Runić, Jasmina
AU  - Murray, Michael
AU  - O'Connor, Kevin 
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/784
AB  - A process for the conversion of post consumer (agricultural) polyethylene (PE) waste to the biodegradable polymer medium chain length polyhydroxyalkanoate (mcl-PHA) is reported here. The thermal treatment of PE in the absence of air (pyrolysis) generated a complex mixture of low molecular weight paraffins with carbon chain lengths from C8 to C32 (PE pyrolysis wax). Several bacterial strains were able to grow and produce PHA from this PE pyrolysis wax. The addition of biosurfactant (rhamnolipids) allowed for greater bacterial growth and PHA accumulation of the tested strains. Some strains were only capable of growth and PHA accumulation in the presence of the biosurfactant. Pseudomonas aeruginosa PAO-1 accumulated the highest level of PHA with almost 25 % of the cell dry weight as PHA when supplied with the PE pyrolysis wax in the presence of rhamnolipids. The change of nitrogen source from ammonium chloride to ammonium nitrate resulted in faster bacterial growth and the earlier onset of PHA accumulation. To our knowledge, this is the first report where PE is used as a starting material for production of a biodegradable polymer.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate
EP  - 4232
IS  - 9
SP  - 4223
VL  - 98
DO  - 10.1007/s00253-013-5489-2
ER  - 

@article{
author = "Guzik, Maciej W. and Kenny, Shane T. and Duane, Gearoid F. and Casey, Eoin and Woods, Trevor and Babu, Ramesh and Nikodinović-Runić, Jasmina and Murray, Michael and O'Connor, Kevin ",
year = "2014",
abstract = "A process for the conversion of post consumer (agricultural) polyethylene (PE) waste to the biodegradable polymer medium chain length polyhydroxyalkanoate (mcl-PHA) is reported here. The thermal treatment of PE in the absence of air (pyrolysis) generated a complex mixture of low molecular weight paraffins with carbon chain lengths from C8 to C32 (PE pyrolysis wax). Several bacterial strains were able to grow and produce PHA from this PE pyrolysis wax. The addition of biosurfactant (rhamnolipids) allowed for greater bacterial growth and PHA accumulation of the tested strains. Some strains were only capable of growth and PHA accumulation in the presence of the biosurfactant. Pseudomonas aeruginosa PAO-1 accumulated the highest level of PHA with almost 25 % of the cell dry weight as PHA when supplied with the PE pyrolysis wax in the presence of rhamnolipids. The change of nitrogen source from ammonium chloride to ammonium nitrate resulted in faster bacterial growth and the earlier onset of PHA accumulation. To our knowledge, this is the first report where PE is used as a starting material for production of a biodegradable polymer.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate",
pages = "4232-4223",
number = "9",
volume = "98",
doi = "10.1007/s00253-013-5489-2"
}

Guzik, M. W., Kenny, S. T., Duane, G. F., Casey, E., Woods, T., Babu, R., Nikodinović-Runić, J., Murray, M.,& O'Connor, K.. (2014). Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate. in Applied Microbiology and Biotechnology
Springer, New York., 98(9), 4223-4232.
https://doi.org/10.1007/s00253-013-5489-2

Guzik MW, Kenny ST, Duane GF, Casey E, Woods T, Babu R, Nikodinović-Runić J, Murray M, O'Connor K. Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate. in Applied Microbiology and Biotechnology. 2014;98(9):4223-4232.
doi:10.1007/s00253-013-5489-2 .

Guzik, Maciej W., Kenny, Shane T., Duane, Gearoid F., Casey, Eoin, Woods, Trevor, Babu, Ramesh, Nikodinović-Runić, Jasmina, Murray, Michael, O'Connor, Kevin , "Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate" in Applied Microbiology and Biotechnology, 98, no. 9 (2014):4223-4232,
https://doi.org/10.1007/s00253-013-5489-2 . .

TY  - JOUR
AU  - Casey, William T.
AU  - Nikodinović-Runić, Jasmina
AU  - Fonseca Garcia, Pilar
AU  - Guzik, Maciej W.
AU  - McGrath, John W.
AU  - Quinn, John P.
AU  - Cagney, Gerard
AU  - Auxiliadora Prieto, Maria
AU  - O'Connor, Kevin 
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/628
AB  - The primary enzyme involved in polyphosphate (polyP) synthesis, polyP kinase (ppk), has been deleted in Pseudomonas putidaKT2440. This has resulted in a threefold to sixfold reduction in polyhydroxyalkanoate (PHA) accumulation compared with the wild type under conditions of nitrogen limitation, with either temperature or oxidative (H2O2) stress, when grown on glucose. The accumulation of PHA by ppk mutant was the same as the wild type under nitrogen-limiting growth conditions. There was no difference in polyP levels between wild-type and ppk strains under all growth conditions tested. In the ppk mutant proteome, polyP kinase (PPK) was undetectable, but up-regulation of the polyp-associated proteins polyP adenosine triphosphate (ATP)/nicotinamide adenine dinucleotide (NAD) kinase (PpnK), a putative polyP adenosine monophosphate (AMP) phosphotransferase (PP_1752), and exopolyphosphatase was observed. ppk strain exhibited significantly retarded growth with glycerol as carbon and energy source (42h of lag period compared with 24h in wild-type strain) but similar growth to the wild-type strain with glucose. Analysis of gene transcription revealed downregulation of glycerol kinase and the glycerol facilitator respectively. Glycerol kinase protein expression was also downregulated in the ppk mutant. The deletion of ppk did not affect motility but reduced biofilm formation. Thus, the knockout of the ppk gene has resulted in a number of phenotypic changes to the mutant without affecting polyP accumulation.
PB  - Wiley, Hoboken
T2  - Environmental Microbiology Reports
T1  - The effect of polyphosphate kinase gene deletion on polyhydroxyalkanoate accumulation and carbon metabolism in Pseudomonas putida KT2440
EP  - 746
IS  - 5
SP  - 740
VL  - 5
DO  - 10.1111/1758-2229.12076
ER  - 

@article{
author = "Casey, William T. and Nikodinović-Runić, Jasmina and Fonseca Garcia, Pilar and Guzik, Maciej W. and McGrath, John W. and Quinn, John P. and Cagney, Gerard and Auxiliadora Prieto, Maria and O'Connor, Kevin ",
year = "2013",
abstract = "The primary enzyme involved in polyphosphate (polyP) synthesis, polyP kinase (ppk), has been deleted in Pseudomonas putidaKT2440. This has resulted in a threefold to sixfold reduction in polyhydroxyalkanoate (PHA) accumulation compared with the wild type under conditions of nitrogen limitation, with either temperature or oxidative (H2O2) stress, when grown on glucose. The accumulation of PHA by ppk mutant was the same as the wild type under nitrogen-limiting growth conditions. There was no difference in polyP levels between wild-type and ppk strains under all growth conditions tested. In the ppk mutant proteome, polyP kinase (PPK) was undetectable, but up-regulation of the polyp-associated proteins polyP adenosine triphosphate (ATP)/nicotinamide adenine dinucleotide (NAD) kinase (PpnK), a putative polyP adenosine monophosphate (AMP) phosphotransferase (PP_1752), and exopolyphosphatase was observed. ppk strain exhibited significantly retarded growth with glycerol as carbon and energy source (42h of lag period compared with 24h in wild-type strain) but similar growth to the wild-type strain with glucose. Analysis of gene transcription revealed downregulation of glycerol kinase and the glycerol facilitator respectively. Glycerol kinase protein expression was also downregulated in the ppk mutant. The deletion of ppk did not affect motility but reduced biofilm formation. Thus, the knockout of the ppk gene has resulted in a number of phenotypic changes to the mutant without affecting polyP accumulation.",
publisher = "Wiley, Hoboken",
journal = "Environmental Microbiology Reports",
title = "The effect of polyphosphate kinase gene deletion on polyhydroxyalkanoate accumulation and carbon metabolism in Pseudomonas putida KT2440",
pages = "746-740",
number = "5",
volume = "5",
doi = "10.1111/1758-2229.12076"
}

Casey, W. T., Nikodinović-Runić, J., Fonseca Garcia, P., Guzik, M. W., McGrath, J. W., Quinn, J. P., Cagney, G., Auxiliadora Prieto, M.,& O'Connor, K.. (2013). The effect of polyphosphate kinase gene deletion on polyhydroxyalkanoate accumulation and carbon metabolism in Pseudomonas putida KT2440. in Environmental Microbiology Reports
Wiley, Hoboken., 5(5), 740-746.
https://doi.org/10.1111/1758-2229.12076

Casey WT, Nikodinović-Runić J, Fonseca Garcia P, Guzik MW, McGrath JW, Quinn JP, Cagney G, Auxiliadora Prieto M, O'Connor K. The effect of polyphosphate kinase gene deletion on polyhydroxyalkanoate accumulation and carbon metabolism in Pseudomonas putida KT2440. in Environmental Microbiology Reports. 2013;5(5):740-746.
doi:10.1111/1758-2229.12076 .

Casey, William T., Nikodinović-Runić, Jasmina, Fonseca Garcia, Pilar, Guzik, Maciej W., McGrath, John W., Quinn, John P., Cagney, Gerard, Auxiliadora Prieto, Maria, O'Connor, Kevin , "The effect of polyphosphate kinase gene deletion on polyhydroxyalkanoate accumulation and carbon metabolism in Pseudomonas putida KT2440" in Environmental Microbiology Reports, 5, no. 5 (2013):740-746,
https://doi.org/10.1111/1758-2229.12076 . .

TY  - JOUR
AU  - Nikodinović-Runić, Jasmina
AU  - Guzik, Maciej
AU  - Kenny, Shane T.
AU  - Babu, Ramesh
AU  - Werker, Alan
AU  - Connor, Kevin E. O.
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/650
AB  - Research into the production of biodegradable polymers has been driven by vision for the most part from changes in policy, in Europe and America. These policies have their origins in the Brundtland Report of 1987, which provides a platform for a more sustainable society. Biodegradable polymers are part of the emerging portfolio of renewable raw materials seeking to deliver environmental, social, and economic benefits. Polyhydroxyalkanoates (PHAs) are naturally-occurring biodegradable-polyesters accumulated by bacteria usually in response to inorganic nutrient limitation in the presence of excess carbon. Most of the early research into PHA accumulation and technology development for industrial-scale production was undertaken using virgin starting materials. For example, polyhydroxybutyrate and copolymers such as polyhydroxybutyrate-co-valerate are produced today at industrial scale from corn-derived glucose. However, in recent years, research has been undertaken to convert domestic and industrial wastes to PHA. These wastes in today's context are residuals seen by a growing body of stakeholders as platform resources for a biobased society. In the present review, we consider residuals from food, plastic, forest and lignocellulosic, and biodiesel manufacturing (glycerol). Thus, this review seeks to gain perspective of opportunities from literature reporting the production of PHA from carbon-rich residuals as feedstocks. A discussion on approaches and context for PHA production with reference to pure- and mixed-culture technologies is provided. Literature reports advocate results of the promise of waste conversion to PHA. However, the vast majority of studies on waste to PHA is at laboratory scale. The questions of surmounting the technical and political hurdles to industrialization are generally left unanswered. There are a limited number of studies that have progressed into fermentors and a dearth of pilot-scale demonstration. A number of fermentation studies show that biomass and PHA productivity can be increased, and sometimes dramatically, in a fermentor. The relevant application-specific properties of the polymers from the wastes studied and the effect of altered-waste composition on polymer properties are generally not well reported and would greatly benefit the progress of the research as high productivity is of limited value without the context of requisite case-specific polymer properties. The proposed use of a waste residual is advantageous from a life cycle viewpoint as it removes the direct or indirect effect of PHA production on land usage and food production. However, the question, of how economic drivers will promote or hinder advancements to demonstration scale, when wastes generally become understood as resources for a biobased society, hangs today in the balance due to a lack of shared vision and the legacy of mistakes made with first generation bioproducts.
PB  - Elsevier Academic Press Inc, San Diego
T2  - Advances in Applied Microbiology, Vol 84
T1  - Carbon-Rich Wastes as Feedstocks for Biodegradable Polymer (Polyhydroxyalkanoate) Production Using Bacteria
EP  - 200
SP  - 139
VL  - 84
DO  - 10.1016/B978-0-12-407673-0.00004-7
ER  - 

@article{
author = "Nikodinović-Runić, Jasmina and Guzik, Maciej and Kenny, Shane T. and Babu, Ramesh and Werker, Alan and Connor, Kevin E. O.",
year = "2013",
abstract = "Research into the production of biodegradable polymers has been driven by vision for the most part from changes in policy, in Europe and America. These policies have their origins in the Brundtland Report of 1987, which provides a platform for a more sustainable society. Biodegradable polymers are part of the emerging portfolio of renewable raw materials seeking to deliver environmental, social, and economic benefits. Polyhydroxyalkanoates (PHAs) are naturally-occurring biodegradable-polyesters accumulated by bacteria usually in response to inorganic nutrient limitation in the presence of excess carbon. Most of the early research into PHA accumulation and technology development for industrial-scale production was undertaken using virgin starting materials. For example, polyhydroxybutyrate and copolymers such as polyhydroxybutyrate-co-valerate are produced today at industrial scale from corn-derived glucose. However, in recent years, research has been undertaken to convert domestic and industrial wastes to PHA. These wastes in today's context are residuals seen by a growing body of stakeholders as platform resources for a biobased society. In the present review, we consider residuals from food, plastic, forest and lignocellulosic, and biodiesel manufacturing (glycerol). Thus, this review seeks to gain perspective of opportunities from literature reporting the production of PHA from carbon-rich residuals as feedstocks. A discussion on approaches and context for PHA production with reference to pure- and mixed-culture technologies is provided. Literature reports advocate results of the promise of waste conversion to PHA. However, the vast majority of studies on waste to PHA is at laboratory scale. The questions of surmounting the technical and political hurdles to industrialization are generally left unanswered. There are a limited number of studies that have progressed into fermentors and a dearth of pilot-scale demonstration. A number of fermentation studies show that biomass and PHA productivity can be increased, and sometimes dramatically, in a fermentor. The relevant application-specific properties of the polymers from the wastes studied and the effect of altered-waste composition on polymer properties are generally not well reported and would greatly benefit the progress of the research as high productivity is of limited value without the context of requisite case-specific polymer properties. The proposed use of a waste residual is advantageous from a life cycle viewpoint as it removes the direct or indirect effect of PHA production on land usage and food production. However, the question, of how economic drivers will promote or hinder advancements to demonstration scale, when wastes generally become understood as resources for a biobased society, hangs today in the balance due to a lack of shared vision and the legacy of mistakes made with first generation bioproducts.",
publisher = "Elsevier Academic Press Inc, San Diego",
journal = "Advances in Applied Microbiology, Vol 84",
title = "Carbon-Rich Wastes as Feedstocks for Biodegradable Polymer (Polyhydroxyalkanoate) Production Using Bacteria",
pages = "200-139",
volume = "84",
doi = "10.1016/B978-0-12-407673-0.00004-7"
}

Nikodinović-Runić, J., Guzik, M., Kenny, S. T., Babu, R., Werker, A.,& Connor, K. E. O.. (2013). Carbon-Rich Wastes as Feedstocks for Biodegradable Polymer (Polyhydroxyalkanoate) Production Using Bacteria. in Advances in Applied Microbiology, Vol 84
Elsevier Academic Press Inc, San Diego., 84, 139-200.
https://doi.org/10.1016/B978-0-12-407673-0.00004-7

Nikodinović-Runić J, Guzik M, Kenny ST, Babu R, Werker A, Connor KEO. Carbon-Rich Wastes as Feedstocks for Biodegradable Polymer (Polyhydroxyalkanoate) Production Using Bacteria. in Advances in Applied Microbiology, Vol 84. 2013;84:139-200.
doi:10.1016/B978-0-12-407673-0.00004-7 .

Nikodinović-Runić, Jasmina, Guzik, Maciej, Kenny, Shane T., Babu, Ramesh, Werker, Alan, Connor, Kevin E. O., "Carbon-Rich Wastes as Feedstocks for Biodegradable Polymer (Polyhydroxyalkanoate) Production Using Bacteria" in Advances in Applied Microbiology, Vol 84, 84 (2013):139-200,
https://doi.org/10.1016/B978-0-12-407673-0.00004-7 . .