TY  - CONF
AU  - Pavić, Aleksandar
AU  - Đuriš, Jelena
AU  - Vukotić, Goran
AU  - Obradović, Mina
AU  - Plačkić, Nikola
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2379
AB  - Fungal infections, once considered a rare disease,
have become an everyday problem in modern
societies, posing major challenges to global
health. It is estimated that more than one billion
people are affected by fungal infections and 1.6
million people succumb to these diseases every
year. Of the 600 species of fungi capable of causing
infections in humans, species of the genus
Candida cause more than 85% of infections, especially
C. albicans, which has become a serious
threat to human health in immunocompromised
and immunosuppressed individuals. Unfortunately,
the current arsenal of clinical drugs relies
on only four classes of approved drugs (polyenes,
azoles, echinocandins and allylamines), which
are only partially effective, resulting in incomplete
eradication of the fungal infection. In
addition, the serious side effects, ranging from
systemic or organ-specific toxicity to poor bioavailability
and low activity, significantly hamper
the clinical use of antifungals. These problems
call for new effective and safe antifungal agents,but also for appropriate preclinical models to accurately
study potential adverse effects on the
human population and test their efficacy against
fungal infections. In this sense, zebrafish (Danio
rerio) embryos have become one of the most
powerful preclinical animal models in infection
biology and drug discovery, offering the unique
opportunity to simultaneously monitor the safety
and efficacy of the applied molecule in real
time. With the aim of providing a preclinical platform
for the identification of new safe antifungal
drugs to effectively control C. albicans infection,
we comprehensively tested the toxicity of 13
clinical antifungal drugs in the zebrafish embryo
model. The 21 toxicity endpoints, including
survival, teratogenicity, cardiotoxicity and hepatotoxicity,
were evaluated and compared with
adverse effects described in rats and humans. Of
the clinical drugs, the efficacy of fluconazole and
voriconazole was evaluated in the zebrafish - C.
albicans model of systemic and wound biofilm
infection.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - EMPOWERING ANTIFUNGAL DRUGS DISCOVERY THROUGH THE ZEBRAFISH-INFECTIOUS DISEASES MODELLING
EP  - 140
SP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2379
ER  - 

@conference{
author = "Pavić, Aleksandar and Đuriš, Jelena and Vukotić, Goran and Obradović, Mina and Plačkić, Nikola",
year = "2024",
abstract = "Fungal infections, once considered a rare disease,
have become an everyday problem in modern
societies, posing major challenges to global
health. It is estimated that more than one billion
people are affected by fungal infections and 1.6
million people succumb to these diseases every
year. Of the 600 species of fungi capable of causing
infections in humans, species of the genus
Candida cause more than 85% of infections, especially
C. albicans, which has become a serious
threat to human health in immunocompromised
and immunosuppressed individuals. Unfortunately,
the current arsenal of clinical drugs relies
on only four classes of approved drugs (polyenes,
azoles, echinocandins and allylamines), which
are only partially effective, resulting in incomplete
eradication of the fungal infection. In
addition, the serious side effects, ranging from
systemic or organ-specific toxicity to poor bioavailability
and low activity, significantly hamper
the clinical use of antifungals. These problems
call for new effective and safe antifungal agents,but also for appropriate preclinical models to accurately
study potential adverse effects on the
human population and test their efficacy against
fungal infections. In this sense, zebrafish (Danio
rerio) embryos have become one of the most
powerful preclinical animal models in infection
biology and drug discovery, offering the unique
opportunity to simultaneously monitor the safety
and efficacy of the applied molecule in real
time. With the aim of providing a preclinical platform
for the identification of new safe antifungal
drugs to effectively control C. albicans infection,
we comprehensively tested the toxicity of 13
clinical antifungal drugs in the zebrafish embryo
model. The 21 toxicity endpoints, including
survival, teratogenicity, cardiotoxicity and hepatotoxicity,
were evaluated and compared with
adverse effects described in rats and humans. Of
the clinical drugs, the efficacy of fluconazole and
voriconazole was evaluated in the zebrafish - C.
albicans model of systemic and wound biofilm
infection.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "EMPOWERING ANTIFUNGAL DRUGS DISCOVERY THROUGH THE ZEBRAFISH-INFECTIOUS DISEASES MODELLING",
pages = "140-140",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2379"
}

Pavić, A., Đuriš, J., Vukotić, G., Obradović, M.,& Plačkić, N.. (2024). EMPOWERING ANTIFUNGAL DRUGS DISCOVERY THROUGH THE ZEBRAFISH-INFECTIOUS DISEASES MODELLING. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 140-140.
https://hdl.handle.net/21.15107/rcub_imagine_2379

Pavić A, Đuriš J, Vukotić G, Obradović M, Plačkić N. EMPOWERING ANTIFUNGAL DRUGS DISCOVERY THROUGH THE ZEBRAFISH-INFECTIOUS DISEASES MODELLING. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:140-140.
https://hdl.handle.net/21.15107/rcub_imagine_2379 .

Pavić, Aleksandar, Đuriš, Jelena, Vukotić, Goran, Obradović, Mina, Plačkić, Nikola, "EMPOWERING ANTIFUNGAL DRUGS DISCOVERY THROUGH THE ZEBRAFISH-INFECTIOUS DISEASES MODELLING" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):140-140,
https://hdl.handle.net/21.15107/rcub_imagine_2379 .

TY  - CONF
AU  - Plačkić, Nikola
AU  - Kljajević, Nemanja
AU  - Obradović, Mina
AU  - Kekić, Dušan
AU  - Gajić, Ina
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2383
AB  - Anti-microbial drug resistance (AMR) is one of
the global health threats caused by the misuse
of drugs typically used to treat microbial
infections in humans, animals and plants. AMR
in nosocomial infections not only significantly
hinders treatment and endangers the patients’
lives, but also elevates the costs of healthcare.
Multiple research approaches have been initiated
to combat AMR, and one promising method
is bacteriophage therapy. Bacteriophages (phages)
are viruses that naturally exploit bacteria as
their hosts for replication and can cause cell lysis,
which makes them promising candidates for
treating the infections that do not respond to
conventional antibiotic therapies. In this study,
we screened wastewater samples from four
different collectors in Belgrade urban area for
bacteriophages active against clinically isolated
strains of two biofilm-producing bacteria that
readily persist in hospital environment - Klebsiella
pneumoniae (6 strains) and Pseudomonas aeruginosa
(2 strains). Wastewaters were screened
for phage presence through phage enrichment
process, in which bacteria were grown in a mixture
of water samples and nutrient-rich broth.
Obtained cultures were screened for antimicrobial
activity against the respective host strains,
and candidates were subjected to a first-round
plaque assay to detect the phages. Finally, the
activity of all the candidates was tested against
all strains of the same species to gain the first insight
into their host range. We discovered 20 potentially
distinct bacteriophages active against
K. pneumoniae strains and two potentially different
candidates targeting P. aeruginosa. Notably,
one phage exhibited activity against all tested K.
pneumoniae strains, and four were active against
5 out of 6 tested strains. Among 22 candidates in
total, five showed depolymerizing activity, indicating
promise in combating biofilm formation.
Currently, isolation of new phages, as well as purification
and host range analysis is underway for
several candidates targeting K. pneumoniae and
two targeting P. aeruginosa strains.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - NOVEL BACTERIOPHAGE ISOLATION FROM BELGRADE WASTEWATERS
EP  - 148
SP  - 148
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2383
ER  - 

@conference{
author = "Plačkić, Nikola and Kljajević, Nemanja and Obradović, Mina and Kekić, Dušan and Gajić, Ina and Stanisavljević, Nemanja and Vukotić, Goran",
year = "2024",
abstract = "Anti-microbial drug resistance (AMR) is one of
the global health threats caused by the misuse
of drugs typically used to treat microbial
infections in humans, animals and plants. AMR
in nosocomial infections not only significantly
hinders treatment and endangers the patients’
lives, but also elevates the costs of healthcare.
Multiple research approaches have been initiated
to combat AMR, and one promising method
is bacteriophage therapy. Bacteriophages (phages)
are viruses that naturally exploit bacteria as
their hosts for replication and can cause cell lysis,
which makes them promising candidates for
treating the infections that do not respond to
conventional antibiotic therapies. In this study,
we screened wastewater samples from four
different collectors in Belgrade urban area for
bacteriophages active against clinically isolated
strains of two biofilm-producing bacteria that
readily persist in hospital environment - Klebsiella
pneumoniae (6 strains) and Pseudomonas aeruginosa
(2 strains). Wastewaters were screened
for phage presence through phage enrichment
process, in which bacteria were grown in a mixture
of water samples and nutrient-rich broth.
Obtained cultures were screened for antimicrobial
activity against the respective host strains,
and candidates were subjected to a first-round
plaque assay to detect the phages. Finally, the
activity of all the candidates was tested against
all strains of the same species to gain the first insight
into their host range. We discovered 20 potentially
distinct bacteriophages active against
K. pneumoniae strains and two potentially different
candidates targeting P. aeruginosa. Notably,
one phage exhibited activity against all tested K.
pneumoniae strains, and four were active against
5 out of 6 tested strains. Among 22 candidates in
total, five showed depolymerizing activity, indicating
promise in combating biofilm formation.
Currently, isolation of new phages, as well as purification
and host range analysis is underway for
several candidates targeting K. pneumoniae and
two targeting P. aeruginosa strains.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "NOVEL BACTERIOPHAGE ISOLATION FROM BELGRADE WASTEWATERS",
pages = "148-148",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2383"
}

Plačkić, N., Kljajević, N., Obradović, M., Kekić, D., Gajić, I., Stanisavljević, N.,& Vukotić, G.. (2024). NOVEL BACTERIOPHAGE ISOLATION FROM BELGRADE WASTEWATERS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 148-148.
https://hdl.handle.net/21.15107/rcub_imagine_2383

Plačkić N, Kljajević N, Obradović M, Kekić D, Gajić I, Stanisavljević N, Vukotić G. NOVEL BACTERIOPHAGE ISOLATION FROM BELGRADE WASTEWATERS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:148-148.
https://hdl.handle.net/21.15107/rcub_imagine_2383 .

Plačkić, Nikola, Kljajević, Nemanja, Obradović, Mina, Kekić, Dušan, Gajić, Ina, Stanisavljević, Nemanja, Vukotić, Goran, "NOVEL BACTERIOPHAGE ISOLATION FROM BELGRADE WASTEWATERS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):148-148,
https://hdl.handle.net/21.15107/rcub_imagine_2383 .

TY  - CONF
AU  - Vukotić, Goran
AU  - Obradović, Mina
AU  - Plačkić, Nikola
AU  - Kljajević, Nemanja
AU  - Pavić, Aleksandar
AU  - Kekić, Dušan
AU  - Gajić, Ina
AU  - Kojić, Milan
AU  - Stanisavljević, Nemanja
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2375
AB  - Antimicrobial resistance (AMR) arises when
bacteria and other microbes stop responding
to medications. AMR is now recognized as one
of serious global health threats, repeatedly
appearing in the World Health Organization’s
(WHO) lists of urgent global health challenges,
including the 2024 list. It is taking a fatal toll
– nearly 5 million deaths globally per year are
associated with AMR, encompassing 1.27 million
directly attributed to AMR. The COVID-19
pandemic paved the way for aggravation of
bacterial AMR – primarily due to enhancement
in unspecific and unjustified prescription and
use of broad-spectrum antibiotics, resulting in
what is now recognized as „silent pandemic of
AMR“. Bacteriophages (phages) are natural and
specific predators of bacteria - viruses that can
infect, replicate inside and lyse arguably any
bacteria. Their therapeutic potential is being
hastily evaluated through different approaches:
in silico, in vitro, ex vivo and in vivo – in laboratory
animals as well as in human case and clinical
studies. Although the results are promising,bacteria rapidly develop resistance against
phages, which why the isolation and research
of new phages is needed. Our work is concentrated
on three bacterial species for which critical
priority by WHO has been declared – carbapenem-
resistant Acinetobacter baumannii,
Pseudomonas aeruginosa and Klebsiella pneumoniae.
Twenty distinct pathogenic strains of
A. baumannii, 6 K. pneumoniae and 6 P. aeruginosa
were used as targets for bacteriophage
isolation, and total of 14, 22 and 8 potentially
distinct phages were collected, respectively. All
strains were nosocomial isolates obtained from
various tissues, including from terminally ill patients.
Six phages were characterized in detail.
In particular, phage vB_AbaM_ISTD was applied
against A. baumannii in zebrafish embryo
model of systemic infection, and demonstrated
powerful therapeutic potential, eradicating the
infection. Interestingly, its DNA was characterized
with highly modified thymidine (amassing
1228 Da), making it the largest non-canonical
deoxynucleoside reported so far.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE
EP  - 121
SP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2375
ER  - 

@conference{
author = "Vukotić, Goran and Obradović, Mina and Plačkić, Nikola and Kljajević, Nemanja and Pavić, Aleksandar and Kekić, Dušan and Gajić, Ina and Kojić, Milan and Stanisavljević, Nemanja",
year = "2024",
abstract = "Antimicrobial resistance (AMR) arises when
bacteria and other microbes stop responding
to medications. AMR is now recognized as one
of serious global health threats, repeatedly
appearing in the World Health Organization’s
(WHO) lists of urgent global health challenges,
including the 2024 list. It is taking a fatal toll
– nearly 5 million deaths globally per year are
associated with AMR, encompassing 1.27 million
directly attributed to AMR. The COVID-19
pandemic paved the way for aggravation of
bacterial AMR – primarily due to enhancement
in unspecific and unjustified prescription and
use of broad-spectrum antibiotics, resulting in
what is now recognized as „silent pandemic of
AMR“. Bacteriophages (phages) are natural and
specific predators of bacteria - viruses that can
infect, replicate inside and lyse arguably any
bacteria. Their therapeutic potential is being
hastily evaluated through different approaches:
in silico, in vitro, ex vivo and in vivo – in laboratory
animals as well as in human case and clinical
studies. Although the results are promising,bacteria rapidly develop resistance against
phages, which why the isolation and research
of new phages is needed. Our work is concentrated
on three bacterial species for which critical
priority by WHO has been declared – carbapenem-
resistant Acinetobacter baumannii,
Pseudomonas aeruginosa and Klebsiella pneumoniae.
Twenty distinct pathogenic strains of
A. baumannii, 6 K. pneumoniae and 6 P. aeruginosa
were used as targets for bacteriophage
isolation, and total of 14, 22 and 8 potentially
distinct phages were collected, respectively. All
strains were nosocomial isolates obtained from
various tissues, including from terminally ill patients.
Six phages were characterized in detail.
In particular, phage vB_AbaM_ISTD was applied
against A. baumannii in zebrafish embryo
model of systemic infection, and demonstrated
powerful therapeutic potential, eradicating the
infection. Interestingly, its DNA was characterized
with highly modified thymidine (amassing
1228 Da), making it the largest non-canonical
deoxynucleoside reported so far.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE",
pages = "121-121",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2375"
}

Vukotić, G., Obradović, M., Plačkić, N., Kljajević, N., Pavić, A., Kekić, D., Gajić, I., Kojić, M.,& Stanisavljević, N.. (2024). BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 121-121.
https://hdl.handle.net/21.15107/rcub_imagine_2375

Vukotić G, Obradović M, Plačkić N, Kljajević N, Pavić A, Kekić D, Gajić I, Kojić M, Stanisavljević N. BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:121-121.
https://hdl.handle.net/21.15107/rcub_imagine_2375 .

Vukotić, Goran, Obradović, Mina, Plačkić, Nikola, Kljajević, Nemanja, Pavić, Aleksandar, Kekić, Dušan, Gajić, Ina, Kojić, Milan, Stanisavljević, Nemanja, "BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):121-121,
https://hdl.handle.net/21.15107/rcub_imagine_2375 .

TY  - CONF
AU  - Malešević, Milka
AU  - Stanisavljević, Nemanja
AU  - Rašić, Slađan
AU  - Vukotić, Goran
AU  - Gardijan, Lazar
AU  - Obradović, Mina
AU  - Kojić, Milan
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2150
AB  - Introduction: Brevibacillus laterosporus is a promising microbiological agent that can be used to prevent and control destructive diseases affecting honey bee colonies. In the presentstudy, the short-term
effect of the B. laterosporus BGSP11 bee diet on microbiota and mycobiota was investigated.
Methods: The honey bee diet was supplemented with spores of B. laterosporus BGSP11 at a concentration of 1×108 CFU/mL in sucrose solution. Metabarcoding analysis of the bee microbial community profile was performed based on 16S RNA (bacteriobiota) and Internally Transcribes Spacer (ITS) region
(mycobiota) obtained using MiSeq Illumina sequencing. The QIIME2 v2021.4 pipeline was used to analyze the obtained amplicon data library.
Results: The results show that the BGSP11 bee diet slightly altered the bee microbiota and did not lead
to potentially harmful changes in the bacterial microbiota. Moreover, it can potentially induce positive
changes, mainly reflected in the reduction of opportunistic bacteria. On the other hand, the treatment
had a greater effect on mycobiota. However, the changesin the bee mycobiome caused by the treatment
cannot be considered a priori as beneficial or harmful,since the interaction between the bee and its mycobiome is not sufficiently studied. The observed positive changes in the bee mycobiome are mainly
reflected in the reduction of phytopathogenic fungi that may affect the organoleptic and techno-functional properties of honey.
Conclusion: This pilot study suggests that the introduction of BGSP11 in beekeeping practice as a biological agent could be considered due to no harmful effects observed on the microbiota of bees.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Short-term effect of Brevibacillus laterosporus supplemented diet on worker honey bee microbiome
EP  - 112
SP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2150
ER  - 

@conference{
author = "Malešević, Milka and Stanisavljević, Nemanja and Rašić, Slađan and Vukotić, Goran and Gardijan, Lazar and Obradović, Mina and Kojić, Milan",
year = "2023",
abstract = "Introduction: Brevibacillus laterosporus is a promising microbiological agent that can be used to prevent and control destructive diseases affecting honey bee colonies. In the presentstudy, the short-term
effect of the B. laterosporus BGSP11 bee diet on microbiota and mycobiota was investigated.
Methods: The honey bee diet was supplemented with spores of B. laterosporus BGSP11 at a concentration of 1×108 CFU/mL in sucrose solution. Metabarcoding analysis of the bee microbial community profile was performed based on 16S RNA (bacteriobiota) and Internally Transcribes Spacer (ITS) region
(mycobiota) obtained using MiSeq Illumina sequencing. The QIIME2 v2021.4 pipeline was used to analyze the obtained amplicon data library.
Results: The results show that the BGSP11 bee diet slightly altered the bee microbiota and did not lead
to potentially harmful changes in the bacterial microbiota. Moreover, it can potentially induce positive
changes, mainly reflected in the reduction of opportunistic bacteria. On the other hand, the treatment
had a greater effect on mycobiota. However, the changesin the bee mycobiome caused by the treatment
cannot be considered a priori as beneficial or harmful,since the interaction between the bee and its mycobiome is not sufficiently studied. The observed positive changes in the bee mycobiome are mainly
reflected in the reduction of phytopathogenic fungi that may affect the organoleptic and techno-functional properties of honey.
Conclusion: This pilot study suggests that the introduction of BGSP11 in beekeeping practice as a biological agent could be considered due to no harmful effects observed on the microbiota of bees.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Short-term effect of Brevibacillus laterosporus supplemented diet on worker honey bee microbiome",
pages = "112-112",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2150"
}

Malešević, M., Stanisavljević, N., Rašić, S., Vukotić, G., Gardijan, L., Obradović, M.,& Kojić, M.. (2023). Short-term effect of Brevibacillus laterosporus supplemented diet on worker honey bee microbiome. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 112-112.
https://hdl.handle.net/21.15107/rcub_imagine_2150

Malešević M, Stanisavljević N, Rašić S, Vukotić G, Gardijan L, Obradović M, Kojić M. Short-term effect of Brevibacillus laterosporus supplemented diet on worker honey bee microbiome. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:112-112.
https://hdl.handle.net/21.15107/rcub_imagine_2150 .

Malešević, Milka, Stanisavljević, Nemanja, Rašić, Slađan, Vukotić, Goran, Gardijan, Lazar, Obradović, Mina, Kojić, Milan, "Short-term effect of Brevibacillus laterosporus supplemented diet on worker honey bee microbiome" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):112-112,
https://hdl.handle.net/21.15107/rcub_imagine_2150 .

TY  - CONF
AU  - Obradović, Mina
AU  - Pavić, Aleksandra
AU  - Vukotić, Goran
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2137
AB  - Introduction: There is an urgent demand for the development of new therapeutic approachesto combat multidrug-resistant Acinetobacter baumannii, and bacteriophages appear to be a highly promising
solution. Phages are suitable to precisely target the infection-causing bacteria without disrupting the
beneficial microbiota. The zebrafish (Danio rerio) embryo model represents an insightful animal model
for preclinical studying of various infectious diseases and for discovery of novel safe and effective antimicrobial drugs.
Methods: Systemic bacterial infection was established by microinjection of 2000 cells of nosocomial
carbapenem-resistant A. baumannii strain 6077/12 into the bloodstream of 48 hour old zebrafish embryos. Infected embryos were treated by parenteral administration of 4 different doses (10, 50, 100, 500
PFU) of bacteriophage vB_AbaM_ISTD at 6 hours after infection (hpi). Efficacy of treatment was evaluated according to embryo survival, morphological malformations and bacterial burden (CFU) over a 3-
day period.
Results: A. baumannii-infected embryos treated with bacteriophage resulted with 100% survival rate,
while 70% of untreated embryos survived to 24 hpi and none to the end of the experiment. Viable bacterial cell count and embryo morphology observations indicated that the administered phage effectively reduced A. baumanii infection in vivo. The most effective dose was 500 PFU, decreasing the bacterial
load by 3.09 log units during 24 hpi, while lower bacteriophage doses(10, 50 and 100 PFU) produced less
prominent, but also significant bacterial reduction of 2.10, 2.19 and 2.67 log units, respectively.
Conclusion: Parentheral administration of phage ISTD demonstrated potent therapeutic activity against
A. baumannii infection in every investigated dose.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Unveiling therapeutic potential of bacteriophage treatment in acinetobacter baumannii-infected zebrafish embryo model
EP  - 83
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2137
ER  - 

@conference{
author = "Obradović, Mina and Pavić, Aleksandra and Vukotić, Goran",
year = "2023",
abstract = "Introduction: There is an urgent demand for the development of new therapeutic approachesto combat multidrug-resistant Acinetobacter baumannii, and bacteriophages appear to be a highly promising
solution. Phages are suitable to precisely target the infection-causing bacteria without disrupting the
beneficial microbiota. The zebrafish (Danio rerio) embryo model represents an insightful animal model
for preclinical studying of various infectious diseases and for discovery of novel safe and effective antimicrobial drugs.
Methods: Systemic bacterial infection was established by microinjection of 2000 cells of nosocomial
carbapenem-resistant A. baumannii strain 6077/12 into the bloodstream of 48 hour old zebrafish embryos. Infected embryos were treated by parenteral administration of 4 different doses (10, 50, 100, 500
PFU) of bacteriophage vB_AbaM_ISTD at 6 hours after infection (hpi). Efficacy of treatment was evaluated according to embryo survival, morphological malformations and bacterial burden (CFU) over a 3-
day period.
Results: A. baumannii-infected embryos treated with bacteriophage resulted with 100% survival rate,
while 70% of untreated embryos survived to 24 hpi and none to the end of the experiment. Viable bacterial cell count and embryo morphology observations indicated that the administered phage effectively reduced A. baumanii infection in vivo. The most effective dose was 500 PFU, decreasing the bacterial
load by 3.09 log units during 24 hpi, while lower bacteriophage doses(10, 50 and 100 PFU) produced less
prominent, but also significant bacterial reduction of 2.10, 2.19 and 2.67 log units, respectively.
Conclusion: Parentheral administration of phage ISTD demonstrated potent therapeutic activity against
A. baumannii infection in every investigated dose.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Unveiling therapeutic potential of bacteriophage treatment in acinetobacter baumannii-infected zebrafish embryo model",
pages = "83-83",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2137"
}

Obradović, M., Pavić, A.,& Vukotić, G.. (2023). Unveiling therapeutic potential of bacteriophage treatment in acinetobacter baumannii-infected zebrafish embryo model. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 83-83.
https://hdl.handle.net/21.15107/rcub_imagine_2137

Obradović M, Pavić A, Vukotić G. Unveiling therapeutic potential of bacteriophage treatment in acinetobacter baumannii-infected zebrafish embryo model. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:83-83.
https://hdl.handle.net/21.15107/rcub_imagine_2137 .

Obradović, Mina, Pavić, Aleksandra, Vukotić, Goran, "Unveiling therapeutic potential of bacteriophage treatment in acinetobacter baumannii-infected zebrafish embryo model" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):83-83,
https://hdl.handle.net/21.15107/rcub_imagine_2137 .

TY  - JOUR
AU  - Obradović, Mina
AU  - Malešević, Milka
AU  - Di Luca, Mariagrazia
AU  - Kekić, Dušan
AU  - Gajić, Ina
AU  - McAuliffe, Olivia
AU  - Neve, Horst
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Kojić, Milan
PY  - 2023
UR  - https://www.mdpi.com/1999-4915/15/3/628
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2065
AB  - Klebsiella pneumoniae is a global health threat and bacteriophages are a potential solution in combating pandrug-resistant K. pneumoniae infections. Two lytic phages, LASTA and SJM3, active against several pandrug-resistant, nosocomial strains of K. pneumoniae were isolated and characterized. Their host range is narrow and latent period is particularly long; however, their lysogenic nature was refuted using both bioinformatic and experimental approaches. Genome sequence analysis clustered them with only two other phages into the new genus Lastavirus. Genomes of LASTA and SJM3 differ in only 13 base pairs, mainly located in tail fiber genes. Individual phages, as well as their cocktail, demonstrated significant bacterial reduction capacity in a time-dependent manner, yielding up to 4 log reduction against planktonic, and up to 2.59 log on biofilm-embedded, cells. Bacteria emerging from the contact with the phages developed resistance and achieved numbers comparable to the growth control after 24 h. The resistance to the phage seems to be of a transient nature and varies significantly between the two phages, as resistance to LASTA remained constant while resensitization to SJM3 was more prominent. Albeit with very few differences, SJM3 performed better than LASTA overall; however, more investigation is needed in order to consider them for therapeutic application.
T2  - Viruses
T1  - Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae
IS  - 3
SP  - 628
VL  - 15
DO  - 10.3390/v15030628
ER  - 

@article{
author = "Obradović, Mina and Malešević, Milka and Di Luca, Mariagrazia and Kekić, Dušan and Gajić, Ina and McAuliffe, Olivia and Neve, Horst and Stanisavljević, Nemanja and Vukotić, Goran and Kojić, Milan",
year = "2023",
abstract = "Klebsiella pneumoniae is a global health threat and bacteriophages are a potential solution in combating pandrug-resistant K. pneumoniae infections. Two lytic phages, LASTA and SJM3, active against several pandrug-resistant, nosocomial strains of K. pneumoniae were isolated and characterized. Their host range is narrow and latent period is particularly long; however, their lysogenic nature was refuted using both bioinformatic and experimental approaches. Genome sequence analysis clustered them with only two other phages into the new genus Lastavirus. Genomes of LASTA and SJM3 differ in only 13 base pairs, mainly located in tail fiber genes. Individual phages, as well as their cocktail, demonstrated significant bacterial reduction capacity in a time-dependent manner, yielding up to 4 log reduction against planktonic, and up to 2.59 log on biofilm-embedded, cells. Bacteria emerging from the contact with the phages developed resistance and achieved numbers comparable to the growth control after 24 h. The resistance to the phage seems to be of a transient nature and varies significantly between the two phages, as resistance to LASTA remained constant while resensitization to SJM3 was more prominent. Albeit with very few differences, SJM3 performed better than LASTA overall; however, more investigation is needed in order to consider them for therapeutic application.",
journal = "Viruses",
title = "Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae",
number = "3",
pages = "628",
volume = "15",
doi = "10.3390/v15030628"
}

Obradović, M., Malešević, M., Di Luca, M., Kekić, D., Gajić, I., McAuliffe, O., Neve, H., Stanisavljević, N., Vukotić, G.,& Kojić, M.. (2023). Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae. in Viruses, 15(3), 628.
https://doi.org/10.3390/v15030628

Obradović M, Malešević M, Di Luca M, Kekić D, Gajić I, McAuliffe O, Neve H, Stanisavljević N, Vukotić G, Kojić M. Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae. in Viruses. 2023;15(3):628.
doi:10.3390/v15030628 .

Obradović, Mina, Malešević, Milka, Di Luca, Mariagrazia, Kekić, Dušan, Gajić, Ina, McAuliffe, Olivia, Neve, Horst, Stanisavljević, Nemanja, Vukotić, Goran, Kojić, Milan, "Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae" in Viruses, 15, no. 3 (2023):628,
https://doi.org/10.3390/v15030628 . .

TY  - DATA
AU  - Obradović, Mina
AU  - Malešević, Milka
AU  - Di Luca, Mariagrazia
AU  - Kekić, Dušan
AU  - Gajić, Ina
AU  - McAuliffe, Olivia
AU  - Neve, Horst
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Kojić, Milan
PY  - 2023
UR  - https://www.mdpi.com/1999-4915/15/3/628
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2065
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2072
T2  - Viruses
T1  - Supplementary data for the article: Obradović, M., Malešević, M., Di Luca, M., Kekić, D., Gajić, I., McAuliffe, O., Neve, H., Stanisavljević, N., Vukotić, G.,& Kojić, M.. (2023). Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae. in Viruses, 15(3), 628. https://doi.org/10.3390/v15030628
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2072
ER  - 

@misc{
author = "Obradović, Mina and Malešević, Milka and Di Luca, Mariagrazia and Kekić, Dušan and Gajić, Ina and McAuliffe, Olivia and Neve, Horst and Stanisavljević, Nemanja and Vukotić, Goran and Kojić, Milan",
year = "2023",
journal = "Viruses",
title = "Supplementary data for the article: Obradović, M., Malešević, M., Di Luca, M., Kekić, D., Gajić, I., McAuliffe, O., Neve, H., Stanisavljević, N., Vukotić, G.,& Kojić, M.. (2023). Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae. in Viruses, 15(3), 628. https://doi.org/10.3390/v15030628",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2072"
}

Obradović, M., Malešević, M., Di Luca, M., Kekić, D., Gajić, I., McAuliffe, O., Neve, H., Stanisavljević, N., Vukotić, G.,& Kojić, M.. (2023). Supplementary data for the article: Obradović, M., Malešević, M., Di Luca, M., Kekić, D., Gajić, I., McAuliffe, O., Neve, H., Stanisavljević, N., Vukotić, G.,& Kojić, M.. (2023). Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae. in Viruses, 15(3), 628. https://doi.org/10.3390/v15030628. in Viruses.
https://hdl.handle.net/21.15107/rcub_imagine_2072

Obradović M, Malešević M, Di Luca M, Kekić D, Gajić I, McAuliffe O, Neve H, Stanisavljević N, Vukotić G, Kojić M. Supplementary data for the article: Obradović, M., Malešević, M., Di Luca, M., Kekić, D., Gajić, I., McAuliffe, O., Neve, H., Stanisavljević, N., Vukotić, G.,& Kojić, M.. (2023). Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae. in Viruses, 15(3), 628. https://doi.org/10.3390/v15030628. in Viruses. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2072 .

Obradović, Mina, Malešević, Milka, Di Luca, Mariagrazia, Kekić, Dušan, Gajić, Ina, McAuliffe, Olivia, Neve, Horst, Stanisavljević, Nemanja, Vukotić, Goran, Kojić, Milan, "Supplementary data for the article: Obradović, M., Malešević, M., Di Luca, M., Kekić, D., Gajić, I., McAuliffe, O., Neve, H., Stanisavljević, N., Vukotić, G.,& Kojić, M.. (2023). Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae. in Viruses, 15(3), 628. https://doi.org/10.3390/v15030628" in Viruses (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2072 .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Malešević, Milka
AU  - Rašić, Slađan
AU  - Vukotić, Goran
AU  - Gardijan, Lazar
AU  - Obradović, Mina
AU  - Kojić, Milan
PY  - 2023
UR  - https://doi.org/10.1080/00218839.2023.2244710
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2063
AB  - In the current study, honey bees’ diet was supplemented with spores of Brevibacillus laterosporus BGSP11 at concentration of 108 CFU/ml in sucrose solution and its short-term effects on their micro- and mycobiota have been analyzed using Illumina MiSeq sequencing. Obtained results indicate that this treatment does not lead to potentially harmful changes in the bacterial microbiome of worker bees, slightly affecting the composition of core microbiota. Moreover, several potentially beneficial changes have been observed. The treatment has led to a significant increase in the abundance of Snodgrassella alvi, and species from Lactobacillus and Bifidobacterium genera which play important roles in protection against several honey bee pathogens. Simultaneously, B. laterosporus enriched diet have led to almost complete eradication of Enterobacteriaceae family, the taxon that contains several putative pathogen species. On the other hand, the treatment affected mycobiota more profoundly, which was expected considering the greater instability compared to microbiota. Although the observed changes in honey bee mycobiome cannot be considered a priori beneficial or harmful, since the interaction between the bee and its mycobiome has not been sufficiently studied, certain beneficial consequences of the treatment have been observed. They are primarily reflected in the reduction of phytopathogenic fungi that can affect the organoleptic and techno-functional characteristics of honey. In addition, before introducing B. laterosporus in beekeeping practice as a biological agent for pathogen control it is necessary to perform more thorough studies of the impact on the honey bee microbiome, immune system, physiology and economic characteristics of honey bee colonies.
T2  - Journal of Apicultural Research
T1  - Short-term effects of Brevibacillus laterosporus supplemented diet on worker honey bee microbiome: a pilot study
DO  - 10.1080/00218839.2023.2244710
ER  - 

@article{
author = "Stanisavljević, Nemanja and Malešević, Milka and Rašić, Slađan and Vukotić, Goran and Gardijan, Lazar and Obradović, Mina and Kojić, Milan",
year = "2023",
abstract = "In the current study, honey bees’ diet was supplemented with spores of Brevibacillus laterosporus BGSP11 at concentration of 108 CFU/ml in sucrose solution and its short-term effects on their micro- and mycobiota have been analyzed using Illumina MiSeq sequencing. Obtained results indicate that this treatment does not lead to potentially harmful changes in the bacterial microbiome of worker bees, slightly affecting the composition of core microbiota. Moreover, several potentially beneficial changes have been observed. The treatment has led to a significant increase in the abundance of Snodgrassella alvi, and species from Lactobacillus and Bifidobacterium genera which play important roles in protection against several honey bee pathogens. Simultaneously, B. laterosporus enriched diet have led to almost complete eradication of Enterobacteriaceae family, the taxon that contains several putative pathogen species. On the other hand, the treatment affected mycobiota more profoundly, which was expected considering the greater instability compared to microbiota. Although the observed changes in honey bee mycobiome cannot be considered a priori beneficial or harmful, since the interaction between the bee and its mycobiome has not been sufficiently studied, certain beneficial consequences of the treatment have been observed. They are primarily reflected in the reduction of phytopathogenic fungi that can affect the organoleptic and techno-functional characteristics of honey. In addition, before introducing B. laterosporus in beekeeping practice as a biological agent for pathogen control it is necessary to perform more thorough studies of the impact on the honey bee microbiome, immune system, physiology and economic characteristics of honey bee colonies.",
journal = "Journal of Apicultural Research",
title = "Short-term effects of Brevibacillus laterosporus supplemented diet on worker honey bee microbiome: a pilot study",
doi = "10.1080/00218839.2023.2244710"
}

Stanisavljević, N., Malešević, M., Rašić, S., Vukotić, G., Gardijan, L., Obradović, M.,& Kojić, M.. (2023). Short-term effects of Brevibacillus laterosporus supplemented diet on worker honey bee microbiome: a pilot study. in Journal of Apicultural Research.
https://doi.org/10.1080/00218839.2023.2244710

Stanisavljević N, Malešević M, Rašić S, Vukotić G, Gardijan L, Obradović M, Kojić M. Short-term effects of Brevibacillus laterosporus supplemented diet on worker honey bee microbiome: a pilot study. in Journal of Apicultural Research. 2023;.
doi:10.1080/00218839.2023.2244710 .

Stanisavljević, Nemanja, Malešević, Milka, Rašić, Slađan, Vukotić, Goran, Gardijan, Lazar, Obradović, Mina, Kojić, Milan, "Short-term effects of Brevibacillus laterosporus supplemented diet on worker honey bee microbiome: a pilot study" in Journal of Apicultural Research (2023),
https://doi.org/10.1080/00218839.2023.2244710 . .

TY  - JOUR
AU  - Gardijan, Lazar
AU  - Miljković, Marija
AU  - Obradović, Mina
AU  - Borović, Branka
AU  - Vukotić, Goran
AU  - Jovanović, Goran
AU  - Kojić, Milan
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1603
AB  - Aims The aim of this study was to construct the improved pMAL expression vector to increase the efficacy of purification of small native peptides and their clear-cut separation from MBP tag. The modifications we introduced can be applied to many expression vectors. Methods and Results To improve the pMAL expression vector, we introduced the His(6) tag and the enterokinase cleavage site (Ek) downstream from the MBP tag and Xa cleavage site on the original vector. For cloning of a desired peptide DNA, the enterokinase site contains a unique BsaBI restriction site adjacent to the original multi-cloning site. This redesigned pMAL vector was optimized for the purification of cytoplasmic (pMALc5HisEk) and periplasmic (pMALp5HisEk) peptides. The purification of native and active peptide (P) was obtained following two-step affinity chromatography. In the first step, the entire MBP-His(6)-Ek-P fusion protein is purified using the Ni-NTA agarose column. This fusion protein was cleaved with active His(6) tagged enterokinase. In the second step, the further purification was performed by column containing the mixture of amylose and Ni-NTA agarose resins. This removes both the MBP-His(6) and His(6)-enterokinase leaving pure native protein in solution. These new vectors and the two-step purification protocol were successfully applied in purification of active native small antimicrobial peptides (AMPs), lactococcin A and human beta-defensin. Conclusions We constructed the improved pMAL expression vectors and established the pipeline and optimal conditions for their use in efficient purification of large amounts of active native small peptides. Significance and Impact of the Study Choice of expression vector impacts on the efficiency of expression and purification of desired proteins. The idea of redesigning pMAL vector was driven by the need for rapid purification of larger amounts of active native AMPs. This newly improved pMAL vector, the cloning strategy, expression conditions and two-step purification protocol represent a unique simple approach which can be applied in every laboratory.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Redesigned pMAL expression vector for easy and fast purification of active native antimicrobial peptides
EP  - 1013
IS  - 2
SP  - 1001
VL  - 133
DO  - 10.1111/jam.15623
ER  - 

@article{
author = "Gardijan, Lazar and Miljković, Marija and Obradović, Mina and Borović, Branka and Vukotić, Goran and Jovanović, Goran and Kojić, Milan",
year = "2022",
abstract = "Aims The aim of this study was to construct the improved pMAL expression vector to increase the efficacy of purification of small native peptides and their clear-cut separation from MBP tag. The modifications we introduced can be applied to many expression vectors. Methods and Results To improve the pMAL expression vector, we introduced the His(6) tag and the enterokinase cleavage site (Ek) downstream from the MBP tag and Xa cleavage site on the original vector. For cloning of a desired peptide DNA, the enterokinase site contains a unique BsaBI restriction site adjacent to the original multi-cloning site. This redesigned pMAL vector was optimized for the purification of cytoplasmic (pMALc5HisEk) and periplasmic (pMALp5HisEk) peptides. The purification of native and active peptide (P) was obtained following two-step affinity chromatography. In the first step, the entire MBP-His(6)-Ek-P fusion protein is purified using the Ni-NTA agarose column. This fusion protein was cleaved with active His(6) tagged enterokinase. In the second step, the further purification was performed by column containing the mixture of amylose and Ni-NTA agarose resins. This removes both the MBP-His(6) and His(6)-enterokinase leaving pure native protein in solution. These new vectors and the two-step purification protocol were successfully applied in purification of active native small antimicrobial peptides (AMPs), lactococcin A and human beta-defensin. Conclusions We constructed the improved pMAL expression vectors and established the pipeline and optimal conditions for their use in efficient purification of large amounts of active native small peptides. Significance and Impact of the Study Choice of expression vector impacts on the efficiency of expression and purification of desired proteins. The idea of redesigning pMAL vector was driven by the need for rapid purification of larger amounts of active native AMPs. This newly improved pMAL vector, the cloning strategy, expression conditions and two-step purification protocol represent a unique simple approach which can be applied in every laboratory.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Redesigned pMAL expression vector for easy and fast purification of active native antimicrobial peptides",
pages = "1013-1001",
number = "2",
volume = "133",
doi = "10.1111/jam.15623"
}

Gardijan, L., Miljković, M., Obradović, M., Borović, B., Vukotić, G., Jovanović, G.,& Kojić, M.. (2022). Redesigned pMAL expression vector for easy and fast purification of active native antimicrobial peptides. in Journal of Applied Microbiology
Wiley, Hoboken., 133(2), 1001-1013.
https://doi.org/10.1111/jam.15623

Gardijan L, Miljković M, Obradović M, Borović B, Vukotić G, Jovanović G, Kojić M. Redesigned pMAL expression vector for easy and fast purification of active native antimicrobial peptides. in Journal of Applied Microbiology. 2022;133(2):1001-1013.
doi:10.1111/jam.15623 .

Gardijan, Lazar, Miljković, Marija, Obradović, Mina, Borović, Branka, Vukotić, Goran, Jovanović, Goran, Kojić, Milan, "Redesigned pMAL expression vector for easy and fast purification of active native antimicrobial peptides" in Journal of Applied Microbiology, 133, no. 2 (2022):1001-1013,
https://doi.org/10.1111/jam.15623 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Terzić-Vidojević, Amarela
AU  - Begović, Jelena
AU  - Golić, Nataša
AU  - Ljubobratović, Uros
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1504
AB  - This research aimed to test whether coating with Lactobacillus paracasei subsp. paracasei BGHN14 homogenate may increase soluble protein and amino acid amount on larval fish feed surface. Total amino acid and protein, as well as taste stimulating and growth promoting amino acid amounts were analyzed in coated feed samples. Results indicated that coating with BGHN14 homogenate increased the amount of soluble proteins and free Glycine on feed surface, as well as the availability of protein-bound (hydroxy-)Proline. This lab-scale research provides the basis for use of lactobacilli as resource efficient source of soluble nutrients for animal feedstuffs.
PB  - Central Fisheries Research Inst, Trabzon
T2  - Turkish Journal of Fisheries and Aquatic Sciences
T1  - Enrichment of Larval Fish Feed with Free Amino Acids and Proteins by Coating with Lactobacillus paracasei subsp. paracasei BGHN14 Homogenate
EP  - 573
IS  - 11
SP  - 569
VL  - 21
DO  - 10.4194/1303-2712-v21_11_05
ER  - 

@article{
author = "Lukić, Jovanka and Stanisavljević, Nemanja and Vukotić, Goran and Terzić-Vidojević, Amarela and Begović, Jelena and Golić, Nataša and Ljubobratović, Uros",
year = "2021",
abstract = "This research aimed to test whether coating with Lactobacillus paracasei subsp. paracasei BGHN14 homogenate may increase soluble protein and amino acid amount on larval fish feed surface. Total amino acid and protein, as well as taste stimulating and growth promoting amino acid amounts were analyzed in coated feed samples. Results indicated that coating with BGHN14 homogenate increased the amount of soluble proteins and free Glycine on feed surface, as well as the availability of protein-bound (hydroxy-)Proline. This lab-scale research provides the basis for use of lactobacilli as resource efficient source of soluble nutrients for animal feedstuffs.",
publisher = "Central Fisheries Research Inst, Trabzon",
journal = "Turkish Journal of Fisheries and Aquatic Sciences",
title = "Enrichment of Larval Fish Feed with Free Amino Acids and Proteins by Coating with Lactobacillus paracasei subsp. paracasei BGHN14 Homogenate",
pages = "573-569",
number = "11",
volume = "21",
doi = "10.4194/1303-2712-v21_11_05"
}

Lukić, J., Stanisavljević, N., Vukotić, G., Terzić-Vidojević, A., Begović, J., Golić, N.,& Ljubobratović, U.. (2021). Enrichment of Larval Fish Feed with Free Amino Acids and Proteins by Coating with Lactobacillus paracasei subsp. paracasei BGHN14 Homogenate. in Turkish Journal of Fisheries and Aquatic Sciences
Central Fisheries Research Inst, Trabzon., 21(11), 569-573.
https://doi.org/10.4194/1303-2712-v21_11_05

Lukić J, Stanisavljević N, Vukotić G, Terzić-Vidojević A, Begović J, Golić N, Ljubobratović U. Enrichment of Larval Fish Feed with Free Amino Acids and Proteins by Coating with Lactobacillus paracasei subsp. paracasei BGHN14 Homogenate. in Turkish Journal of Fisheries and Aquatic Sciences. 2021;21(11):569-573.
doi:10.4194/1303-2712-v21_11_05 .

Lukić, Jovanka, Stanisavljević, Nemanja, Vukotić, Goran, Terzić-Vidojević, Amarela, Begović, Jelena, Golić, Nataša, Ljubobratović, Uros, "Enrichment of Larval Fish Feed with Free Amino Acids and Proteins by Coating with Lactobacillus paracasei subsp. paracasei BGHN14 Homogenate" in Turkish Journal of Fisheries and Aquatic Sciences, 21, no. 11 (2021):569-573,
https://doi.org/10.4194/1303-2712-v21_11_05 . .

TY  - JOUR
AU  - Kekić, Dusan
AU  - Gajić, Ina
AU  - Opavski, Nataša
AU  - Kojić, Milan
AU  - Vukotić, Goran
AU  - Smitran, Aleksandra
AU  - Bosković, Lidija
AU  - Stojković, Marina
AU  - Ranin, Lazar
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1493
AB  - Group B Streptococcus (GBS) is a major cause of neonatal morbidity and mortality. Serbia has not fully implemented preventive measures against GBS neonatal diseases. Therefore, we aimed to assess the maternal GBS colonisation and invasive neonatal disease rate, to reveal the trends of antimicrobial resistance and serotype distribution of GBS from various patient groups. Randomly selected non-invasive (n=991) and all invasive GBS (n=80) collected throughout Serbia from 2015 to 2020 were tested for antimicrobial susceptibility, capsular typing, and hvgA detection. Overall, 877/5621 (15.6%) pregnant women were colonised with GBS. Invasive GBS infections incidence in infants (0.18/1000 live births) showed a decreasing trend (0.3 to 0.1/1000 live births). Type III was overrepresented in infants with invasive infections (n=35, 58.3%), whereas type V predominated among colonised adults (n=224, 25.5%) and those with noninvasive (n=37, 32.5%) and invasive infections (n=8, 40%). The hypervirulent clone III/ST17 was highly associated with invasive infections (n=28, 35%), particularly late-onset disease (n=9, 47.4%), showing an increase from 12.3 to 14.8%. The GBS resistance to erythromycin and clindamycin was 26.7% and 22.1%, respectively, with an upward trend. The emergence of the hypervirulent clone III/ST17 and the escalation in GBS resistance highlight an urgent need for continuous monitoring of GBS infections.
PB  - Nature Research, Berlin
T2  - Scientific Reports
T1  - Trends in molecular characteristics and antimicrobial resistance of group B streptococci: a multicenter study in Serbia, 2015-2020
IS  - 1
VL  - 11
DO  - 10.1038/s41598-020-79354-3
ER  - 

@article{
author = "Kekić, Dusan and Gajić, Ina and Opavski, Nataša and Kojić, Milan and Vukotić, Goran and Smitran, Aleksandra and Bosković, Lidija and Stojković, Marina and Ranin, Lazar",
year = "2021",
abstract = "Group B Streptococcus (GBS) is a major cause of neonatal morbidity and mortality. Serbia has not fully implemented preventive measures against GBS neonatal diseases. Therefore, we aimed to assess the maternal GBS colonisation and invasive neonatal disease rate, to reveal the trends of antimicrobial resistance and serotype distribution of GBS from various patient groups. Randomly selected non-invasive (n=991) and all invasive GBS (n=80) collected throughout Serbia from 2015 to 2020 were tested for antimicrobial susceptibility, capsular typing, and hvgA detection. Overall, 877/5621 (15.6%) pregnant women were colonised with GBS. Invasive GBS infections incidence in infants (0.18/1000 live births) showed a decreasing trend (0.3 to 0.1/1000 live births). Type III was overrepresented in infants with invasive infections (n=35, 58.3%), whereas type V predominated among colonised adults (n=224, 25.5%) and those with noninvasive (n=37, 32.5%) and invasive infections (n=8, 40%). The hypervirulent clone III/ST17 was highly associated with invasive infections (n=28, 35%), particularly late-onset disease (n=9, 47.4%), showing an increase from 12.3 to 14.8%. The GBS resistance to erythromycin and clindamycin was 26.7% and 22.1%, respectively, with an upward trend. The emergence of the hypervirulent clone III/ST17 and the escalation in GBS resistance highlight an urgent need for continuous monitoring of GBS infections.",
publisher = "Nature Research, Berlin",
journal = "Scientific Reports",
title = "Trends in molecular characteristics and antimicrobial resistance of group B streptococci: a multicenter study in Serbia, 2015-2020",
number = "1",
volume = "11",
doi = "10.1038/s41598-020-79354-3"
}

Kekić, D., Gajić, I., Opavski, N., Kojić, M., Vukotić, G., Smitran, A., Bosković, L., Stojković, M.,& Ranin, L.. (2021). Trends in molecular characteristics and antimicrobial resistance of group B streptococci: a multicenter study in Serbia, 2015-2020. in Scientific Reports
Nature Research, Berlin., 11(1).
https://doi.org/10.1038/s41598-020-79354-3

Kekić D, Gajić I, Opavski N, Kojić M, Vukotić G, Smitran A, Bosković L, Stojković M, Ranin L. Trends in molecular characteristics and antimicrobial resistance of group B streptococci: a multicenter study in Serbia, 2015-2020. in Scientific Reports. 2021;11(1).
doi:10.1038/s41598-020-79354-3 .

Kekić, Dusan, Gajić, Ina, Opavski, Nataša, Kojić, Milan, Vukotić, Goran, Smitran, Aleksandra, Bosković, Lidija, Stojković, Marina, Ranin, Lazar, "Trends in molecular characteristics and antimicrobial resistance of group B streptococci: a multicenter study in Serbia, 2015-2020" in Scientific Reports, 11, no. 1 (2021),
https://doi.org/10.1038/s41598-020-79354-3 . .

TY  - JOUR
AU  - Ljubobratović, Uros
AU  - Fazekas, Georgina
AU  - Koljukaj, Alan
AU  - Ristović, Tijana
AU  - Vass, Vivien
AU  - Ardo, Laszlo
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Pesić, Mirjana
AU  - Milincić, Danijel
AU  - Kostić, Aleksandar
AU  - Lukić, Jovanka
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1448
AB  - This study evaluated whether inert feed enriched with Lb. paracasei subsp. paracasei BGHN14 may be used as a weaning diet for first feeding pike-perch larvae. Three experimental groups were weaned from the start of exogenous feeding: two groups were given inert feed enriched with BGHN14 either via 12 h incubation with live BGHN14 cells or via coating with homogenized BGHN14 cells and one group was supplemented non-enriched inert feed. In all three groups Artemia was co-fed with inert feed during weaning. Control group larvae were fed Artemia exclusively during the treatment period. Treatment lasted fourteen days, starting from the 6th day post-hatch (DPH). Larval sampling was performed on the 20th DPH for gene expression and enzyme activity analysis. Larvae were also sampled on the 32nd DPH for morphometric and body composition analysis. Our results showed that weaning of first feeding pike-perch larvae was associated with an increase of fish condition (0.72 +/- 0.12-0.77 +/- 0.11 versus 0.67 +/- 0.11 in controls), but it suppressed skeleton development, according to Col1 mRNA expression (1 +/- 0.51-1.06 +/- 0.36 versus 2.07 +/- 0.53 in controls) and reduced fat deposition (1.25 +/- 0.23-1.49 +/- 0.33 versus 1.84 +/- 0.31% in controls). This presumably reflected lower availability of soluble proteins in microdiet as opposed to live food, along with high leaching rate of amino acids from solid feed particles, as reported in our previous studies. However, skeleton differentiation was not impaired in group weaned on BGHN14 homogenate coated feed (Col1 mRNA expression: 2.68 +/- 0.72), which was enriched in skeleton building and taste stimulating amino acids. These larvae were also presented with substantially higher length (15.28 +/- 2.55 versus 13.93 +/- 2.31 mm in controls) and weight (26.56 +/- 13.83 versus 21.03 +/- 11.25 mg in controls), which correlated with lower trypsin activity (1.06 +/- 0.13 versus 1.43 +/- 0.26 mU/mg of proteins in controls) and an increase of PLA2 to trypsin activity ratio (453.12 +/- 109.36 versus 264.84 +/- 69.03 in controls). Present study suggests that weaning of first feeding pike-perch larvae using BGHN14 homogenate coated microdiet supports skeleton development and improves fish growth.
PB  - Elsevier, Amsterdam
T2  - Aquaculture
T1  - Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding
VL  - 542
DO  - 10.1016/j.aquaculture.2021.736901
ER  - 

@article{
author = "Ljubobratović, Uros and Fazekas, Georgina and Koljukaj, Alan and Ristović, Tijana and Vass, Vivien and Ardo, Laszlo and Stanisavljević, Nemanja and Vukotić, Goran and Pesić, Mirjana and Milincić, Danijel and Kostić, Aleksandar and Lukić, Jovanka",
year = "2021",
abstract = "This study evaluated whether inert feed enriched with Lb. paracasei subsp. paracasei BGHN14 may be used as a weaning diet for first feeding pike-perch larvae. Three experimental groups were weaned from the start of exogenous feeding: two groups were given inert feed enriched with BGHN14 either via 12 h incubation with live BGHN14 cells or via coating with homogenized BGHN14 cells and one group was supplemented non-enriched inert feed. In all three groups Artemia was co-fed with inert feed during weaning. Control group larvae were fed Artemia exclusively during the treatment period. Treatment lasted fourteen days, starting from the 6th day post-hatch (DPH). Larval sampling was performed on the 20th DPH for gene expression and enzyme activity analysis. Larvae were also sampled on the 32nd DPH for morphometric and body composition analysis. Our results showed that weaning of first feeding pike-perch larvae was associated with an increase of fish condition (0.72 +/- 0.12-0.77 +/- 0.11 versus 0.67 +/- 0.11 in controls), but it suppressed skeleton development, according to Col1 mRNA expression (1 +/- 0.51-1.06 +/- 0.36 versus 2.07 +/- 0.53 in controls) and reduced fat deposition (1.25 +/- 0.23-1.49 +/- 0.33 versus 1.84 +/- 0.31% in controls). This presumably reflected lower availability of soluble proteins in microdiet as opposed to live food, along with high leaching rate of amino acids from solid feed particles, as reported in our previous studies. However, skeleton differentiation was not impaired in group weaned on BGHN14 homogenate coated feed (Col1 mRNA expression: 2.68 +/- 0.72), which was enriched in skeleton building and taste stimulating amino acids. These larvae were also presented with substantially higher length (15.28 +/- 2.55 versus 13.93 +/- 2.31 mm in controls) and weight (26.56 +/- 13.83 versus 21.03 +/- 11.25 mg in controls), which correlated with lower trypsin activity (1.06 +/- 0.13 versus 1.43 +/- 0.26 mU/mg of proteins in controls) and an increase of PLA2 to trypsin activity ratio (453.12 +/- 109.36 versus 264.84 +/- 69.03 in controls). Present study suggests that weaning of first feeding pike-perch larvae using BGHN14 homogenate coated microdiet supports skeleton development and improves fish growth.",
publisher = "Elsevier, Amsterdam",
journal = "Aquaculture",
title = "Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding",
volume = "542",
doi = "10.1016/j.aquaculture.2021.736901"
}

Ljubobratović, U., Fazekas, G., Koljukaj, A., Ristović, T., Vass, V., Ardo, L., Stanisavljević, N., Vukotić, G., Pesić, M., Milincić, D., Kostić, A.,& Lukić, J.. (2021). Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding. in Aquaculture
Elsevier, Amsterdam., 542.
https://doi.org/10.1016/j.aquaculture.2021.736901

Ljubobratović U, Fazekas G, Koljukaj A, Ristović T, Vass V, Ardo L, Stanisavljević N, Vukotić G, Pesić M, Milincić D, Kostić A, Lukić J. Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding. in Aquaculture. 2021;542.
doi:10.1016/j.aquaculture.2021.736901 .

Ljubobratović, Uros, Fazekas, Georgina, Koljukaj, Alan, Ristović, Tijana, Vass, Vivien, Ardo, Laszlo, Stanisavljević, Nemanja, Vukotić, Goran, Pesić, Mirjana, Milincić, Danijel, Kostić, Aleksandar, Lukić, Jovanka, "Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding" in Aquaculture, 542 (2021),
https://doi.org/10.1016/j.aquaculture.2021.736901 . .

TY  - JOUR
AU  - Vukotić, Goran
AU  - Obradović, Mina
AU  - Novović, Katarina
AU  - Di Luca, Mariagrazia
AU  - Jovčić, Branko
AU  - Fira, Đorđe
AU  - Neve, Horst
AU  - Kojić, Milan
AU  - McAuliffe, Olivia
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1355
AB  - Acinetobacter baumanniiis a leading cause of healthcare-associated infections worldwide. Its various intrinsic and acquired mechanisms of antibiotic resistance make the therapeutic challenge even more serious. One of the promising alternative treatments that is increasingly highlighted is phage therapy, the therapeutic use of bacteriophages to treat bacterial infections. Two phages active against nosocomial carbapenem-resistantA. baumanniistrain 6077/12, vB_AbaM_ISTD, and vB_AbaM_NOVI, were isolated from Belgrade wastewaters, purified, and concentrated using CsCl gradient ultracentrifugation. The phages were screened against 103 clinical isolates ofA. baumanniifrom a laboratory collection and characterized based on plaque and virion morphology, host range, adsorption rate, and one-step growth curve. Given that phage ISTD showed a broader host range, better adsorption rate, shorter latent period, and larger burst size, its ability to lyse planktonic and biofilm-embedded cells was tested in detail. Phage ISTD yielded a 3.5- and 2-log reduction in planktonic and biofilm-associated viable bacterial cell count, respectively, but the effect was time-dependent. Both phages produced growing turbid halos around plaques indicating the synthesis of depolymerases, enzymes capable of degrading bacterial exopolysaccharides. Halos tested positive for presence of phages in the proximity of the plaque, but not further from the plaque, which indicates that the observed halo enlargement is a consequence of enzyme diffusion through the agar, independently of the phages. This notion was also supported by the growing halos induced by phage preparations applied on pregrown bacterial lawns, indicating that depolymerizing effect was achieved also on non-dividing sensitive cells. Overall, good rates of growth, fast adsorption rate, broad host range, and high depolymerizing activity, as well as antibacterial effectiveness against planktonic and biofilm-associated bacteria, make these phages good candidates for potential application in combatingA. baumanniiinfections.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Medicine
T1  - Characterization, Antibiofilm, and Depolymerizing Activity of Two Phages Active on Carbapenem-ResistantAcinetobacter baumannii
VL  - 7
DO  - 10.3389/fmed.2020.00426
ER  - 

@article{
author = "Vukotić, Goran and Obradović, Mina and Novović, Katarina and Di Luca, Mariagrazia and Jovčić, Branko and Fira, Đorđe and Neve, Horst and Kojić, Milan and McAuliffe, Olivia",
year = "2020",
abstract = "Acinetobacter baumanniiis a leading cause of healthcare-associated infections worldwide. Its various intrinsic and acquired mechanisms of antibiotic resistance make the therapeutic challenge even more serious. One of the promising alternative treatments that is increasingly highlighted is phage therapy, the therapeutic use of bacteriophages to treat bacterial infections. Two phages active against nosocomial carbapenem-resistantA. baumanniistrain 6077/12, vB_AbaM_ISTD, and vB_AbaM_NOVI, were isolated from Belgrade wastewaters, purified, and concentrated using CsCl gradient ultracentrifugation. The phages were screened against 103 clinical isolates ofA. baumanniifrom a laboratory collection and characterized based on plaque and virion morphology, host range, adsorption rate, and one-step growth curve. Given that phage ISTD showed a broader host range, better adsorption rate, shorter latent period, and larger burst size, its ability to lyse planktonic and biofilm-embedded cells was tested in detail. Phage ISTD yielded a 3.5- and 2-log reduction in planktonic and biofilm-associated viable bacterial cell count, respectively, but the effect was time-dependent. Both phages produced growing turbid halos around plaques indicating the synthesis of depolymerases, enzymes capable of degrading bacterial exopolysaccharides. Halos tested positive for presence of phages in the proximity of the plaque, but not further from the plaque, which indicates that the observed halo enlargement is a consequence of enzyme diffusion through the agar, independently of the phages. This notion was also supported by the growing halos induced by phage preparations applied on pregrown bacterial lawns, indicating that depolymerizing effect was achieved also on non-dividing sensitive cells. Overall, good rates of growth, fast adsorption rate, broad host range, and high depolymerizing activity, as well as antibacterial effectiveness against planktonic and biofilm-associated bacteria, make these phages good candidates for potential application in combatingA. baumanniiinfections.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Medicine",
title = "Characterization, Antibiofilm, and Depolymerizing Activity of Two Phages Active on Carbapenem-ResistantAcinetobacter baumannii",
volume = "7",
doi = "10.3389/fmed.2020.00426"
}

Vukotić, G., Obradović, M., Novović, K., Di Luca, M., Jovčić, B., Fira, Đ., Neve, H., Kojić, M.,& McAuliffe, O.. (2020). Characterization, Antibiofilm, and Depolymerizing Activity of Two Phages Active on Carbapenem-ResistantAcinetobacter baumannii. in Frontiers in Medicine
Frontiers Media Sa, Lausanne., 7.
https://doi.org/10.3389/fmed.2020.00426

Vukotić G, Obradović M, Novović K, Di Luca M, Jovčić B, Fira Đ, Neve H, Kojić M, McAuliffe O. Characterization, Antibiofilm, and Depolymerizing Activity of Two Phages Active on Carbapenem-ResistantAcinetobacter baumannii. in Frontiers in Medicine. 2020;7.
doi:10.3389/fmed.2020.00426 .

Vukotić, Goran, Obradović, Mina, Novović, Katarina, Di Luca, Mariagrazia, Jovčić, Branko, Fira, Đorđe, Neve, Horst, Kojić, Milan, McAuliffe, Olivia, "Characterization, Antibiofilm, and Depolymerizing Activity of Two Phages Active on Carbapenem-ResistantAcinetobacter baumannii" in Frontiers in Medicine, 7 (2020),
https://doi.org/10.3389/fmed.2020.00426 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Kosanović, Dejana
AU  - Terzić-Vidojević, Amarela
AU  - Begović, Jelena
AU  - Golić, Nataša
AU  - Jeney, Galina
AU  - Ljubobratović, Uros
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1382
AB  - This study aimed to analyze an impact of Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 supplemented to Anemia franciscana cultivation medium on biochemical profile of hatched nauplii. Impacts of different BGHO1:BGGO6-55 ratios (75:25, 50:50 and 25:75) and their total concentrations (250 and 500 g/kg of Anemia cysts) at different timings of bacteria application (pre- and post-hatching) were examined. The effects were evaluated by quantification of naupliar peptide, soluble protein, phospho-, neutral and unsaturated lipid content. Uni and multifactorial analysis of variance (ANOVA) were used to estimate the effects of treatments relative to control and to model factor interactions, respectively Statistical analysis indicated that posthatching application of 75:25 strain ratio at high dose was associated with an increase of neutral lipid amount. Furthermore, factor interaction profiling identified positive correlation of lacto-bacilli concentration with the level of free amino acids/short peptides, phospho-, neutral and unsaturated lipids, but only at 50:50 strain ratio combination. Application of lower lactobacilli dose at 75:25 strain ratio caused an increase of soluble protein and phospholipid amount. Hypothetically, graded response of Artemia nauplii to lactobacilli supplementation was induced with different BGHO1 doses. It assumedly ranged from stress-response protein synthesis at lower doses to membrane permeability alterations and triglyceride-mediated defense mechanism activation at higher BGHO1 doses. In contrast to lactobacilli supplementation after Anemia hatching, pre-hatching application of lactobacilli was not associated with an increase of Anemia nutritive profile, though again there was a positive association of lactobacilli concentration with nutrient amount. This implicates interference of lactobacilli with the hatching process. Results presented here facilitate the design of future studies aiming to modify Artemia nutritive profile in accordance with nutritional demands of cultivated fish species.
PB  - Elsevier, Amsterdam
T2  - Animal Feed Science and Technology
T1  - Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner
VL  - 259
DO  - 10.1016/j.anifeedsci.2019.114356
ER  - 

@article{
author = "Lukić, Jovanka and Stanisavljević, Nemanja and Vukotić, Goran and Kosanović, Dejana and Terzić-Vidojević, Amarela and Begović, Jelena and Golić, Nataša and Jeney, Galina and Ljubobratović, Uros",
year = "2020",
abstract = "This study aimed to analyze an impact of Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 supplemented to Anemia franciscana cultivation medium on biochemical profile of hatched nauplii. Impacts of different BGHO1:BGGO6-55 ratios (75:25, 50:50 and 25:75) and their total concentrations (250 and 500 g/kg of Anemia cysts) at different timings of bacteria application (pre- and post-hatching) were examined. The effects were evaluated by quantification of naupliar peptide, soluble protein, phospho-, neutral and unsaturated lipid content. Uni and multifactorial analysis of variance (ANOVA) were used to estimate the effects of treatments relative to control and to model factor interactions, respectively Statistical analysis indicated that posthatching application of 75:25 strain ratio at high dose was associated with an increase of neutral lipid amount. Furthermore, factor interaction profiling identified positive correlation of lacto-bacilli concentration with the level of free amino acids/short peptides, phospho-, neutral and unsaturated lipids, but only at 50:50 strain ratio combination. Application of lower lactobacilli dose at 75:25 strain ratio caused an increase of soluble protein and phospholipid amount. Hypothetically, graded response of Artemia nauplii to lactobacilli supplementation was induced with different BGHO1 doses. It assumedly ranged from stress-response protein synthesis at lower doses to membrane permeability alterations and triglyceride-mediated defense mechanism activation at higher BGHO1 doses. In contrast to lactobacilli supplementation after Anemia hatching, pre-hatching application of lactobacilli was not associated with an increase of Anemia nutritive profile, though again there was a positive association of lactobacilli concentration with nutrient amount. This implicates interference of lactobacilli with the hatching process. Results presented here facilitate the design of future studies aiming to modify Artemia nutritive profile in accordance with nutritional demands of cultivated fish species.",
publisher = "Elsevier, Amsterdam",
journal = "Animal Feed Science and Technology",
title = "Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner",
volume = "259",
doi = "10.1016/j.anifeedsci.2019.114356"
}

Lukić, J., Stanisavljević, N., Vukotić, G., Kosanović, D., Terzić-Vidojević, A., Begović, J., Golić, N., Jeney, G.,& Ljubobratović, U.. (2020). Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner. in Animal Feed Science and Technology
Elsevier, Amsterdam., 259.
https://doi.org/10.1016/j.anifeedsci.2019.114356

Lukić J, Stanisavljević N, Vukotić G, Kosanović D, Terzić-Vidojević A, Begović J, Golić N, Jeney G, Ljubobratović U. Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner. in Animal Feed Science and Technology. 2020;259.
doi:10.1016/j.anifeedsci.2019.114356 .

Lukić, Jovanka, Stanisavljević, Nemanja, Vukotić, Goran, Kosanović, Dejana, Terzić-Vidojević, Amarela, Begović, Jelena, Golić, Nataša, Jeney, Galina, Ljubobratović, Uros, "Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner" in Animal Feed Science and Technology, 259 (2020),
https://doi.org/10.1016/j.anifeedsci.2019.114356 . .

TY  - JOUR
AU  - Ljubobratović, Uros
AU  - Kosanović, Dejana
AU  - Demeny, Ferenc Zoltan
AU  - Krajcsovics, Adrienn
AU  - Vukotić, Goran
AU  - Stanisavljević, Nemanja
AU  - Golić, Nataša
AU  - Jeney, Galina
AU  - Lukić, Jovanka
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1402
AB  - Weaning to inert diet in intensively reared pike-perch larvae is confronted with significant fish loss, which prevents successful commercialization of pike-perch production. Achievement of satisfactory feed quality and effective assimilation of nutrients by larval fish is the major challenge in larval production process. Aim of this study was to evaluate whether treatment of live and inert feed with lactobacilli could alleviate growth retardation associated with early weaning of pike-perch reared in recirculating system. Weaning started on 18th day post-hatching (DPH) either as sudden weaning (SW) or by co-administration of Anemia for six days (gradual weaning, GW). Prior to administration to fish, Anemia was treated with Lactobacillus salivarius BGHO1/Lb. reuteri BGGO6-55, while inert feed was treated with Lb. paracasei subsp. paracasei BGHN14/Lb. rhamnosus BGT10. Treatment with lactobacilli slightly raised neutral lipid level in Anemia nauplii, but significantly reduced their content in dry feed. Fish were sampled on the 24th DPH. Survival, morphometric indices, skeleton differentiation, digestive enzyme activity and opportunistic pathogenic bacteria level were assessed in whole fish specimens. GW fish were presented with better survival, body growth and phospholipase A2 (PLA2) activity. Alongside, Vibrio spp. growth was suppressed in these fish and skeleton development was improved, according to Alizarin Red staining and ColIA1/Sparc mRNA expression data. Lactobacilli application in GW fish correlated with an increase of survival, condition factor and growth rate, according to trypsin and chymotrypsin activities, indicating better utilization of dietary proteins for muscle building. In SW fish, lactobacilli elevated chymotrypsin activity, PLA2 to lipase activity ratio and improved survival and ossification, as evident from Alizarin Red staining and ColIA1/Sparc mRNA expression. This indicated improved fatty acid absorption and control of metamorphosis process. Furthermore, lactobacilli suppressed Vibrio spp. growth in SW fish. Aside from demonstrating the ability of lactobacilli to aid weaning in pike-perch larvae, this study indicated that different types of food treatment may direct fish growth in a predictable manner, allowing further cost-effective improvements of larval pike-perch rearing in intensive system.
PB  - Elsevier, Amsterdam
T2  - Aquaculture
T1  - The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae
VL  - 516
DO  - 10.1016/j.aquaculture.2019.734608
ER  - 

@article{
author = "Ljubobratović, Uros and Kosanović, Dejana and Demeny, Ferenc Zoltan and Krajcsovics, Adrienn and Vukotić, Goran and Stanisavljević, Nemanja and Golić, Nataša and Jeney, Galina and Lukić, Jovanka",
year = "2020",
abstract = "Weaning to inert diet in intensively reared pike-perch larvae is confronted with significant fish loss, which prevents successful commercialization of pike-perch production. Achievement of satisfactory feed quality and effective assimilation of nutrients by larval fish is the major challenge in larval production process. Aim of this study was to evaluate whether treatment of live and inert feed with lactobacilli could alleviate growth retardation associated with early weaning of pike-perch reared in recirculating system. Weaning started on 18th day post-hatching (DPH) either as sudden weaning (SW) or by co-administration of Anemia for six days (gradual weaning, GW). Prior to administration to fish, Anemia was treated with Lactobacillus salivarius BGHO1/Lb. reuteri BGGO6-55, while inert feed was treated with Lb. paracasei subsp. paracasei BGHN14/Lb. rhamnosus BGT10. Treatment with lactobacilli slightly raised neutral lipid level in Anemia nauplii, but significantly reduced their content in dry feed. Fish were sampled on the 24th DPH. Survival, morphometric indices, skeleton differentiation, digestive enzyme activity and opportunistic pathogenic bacteria level were assessed in whole fish specimens. GW fish were presented with better survival, body growth and phospholipase A2 (PLA2) activity. Alongside, Vibrio spp. growth was suppressed in these fish and skeleton development was improved, according to Alizarin Red staining and ColIA1/Sparc mRNA expression data. Lactobacilli application in GW fish correlated with an increase of survival, condition factor and growth rate, according to trypsin and chymotrypsin activities, indicating better utilization of dietary proteins for muscle building. In SW fish, lactobacilli elevated chymotrypsin activity, PLA2 to lipase activity ratio and improved survival and ossification, as evident from Alizarin Red staining and ColIA1/Sparc mRNA expression. This indicated improved fatty acid absorption and control of metamorphosis process. Furthermore, lactobacilli suppressed Vibrio spp. growth in SW fish. Aside from demonstrating the ability of lactobacilli to aid weaning in pike-perch larvae, this study indicated that different types of food treatment may direct fish growth in a predictable manner, allowing further cost-effective improvements of larval pike-perch rearing in intensive system.",
publisher = "Elsevier, Amsterdam",
journal = "Aquaculture",
title = "The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae",
volume = "516",
doi = "10.1016/j.aquaculture.2019.734608"
}

Ljubobratović, U., Kosanović, D., Demeny, F. Z., Krajcsovics, A., Vukotić, G., Stanisavljević, N., Golić, N., Jeney, G.,& Lukić, J.. (2020). The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae. in Aquaculture
Elsevier, Amsterdam., 516.
https://doi.org/10.1016/j.aquaculture.2019.734608

Ljubobratović U, Kosanović D, Demeny FZ, Krajcsovics A, Vukotić G, Stanisavljević N, Golić N, Jeney G, Lukić J. The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae. in Aquaculture. 2020;516.
doi:10.1016/j.aquaculture.2019.734608 .

Ljubobratović, Uros, Kosanović, Dejana, Demeny, Ferenc Zoltan, Krajcsovics, Adrienn, Vukotić, Goran, Stanisavljević, Nemanja, Golić, Nataša, Jeney, Galina, Lukić, Jovanka, "The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae" in Aquaculture, 516 (2020),
https://doi.org/10.1016/j.aquaculture.2019.734608 . .

TY  - JOUR
AU  - Pesić, Mirjana B.
AU  - Milincić, Danijel D.
AU  - Kostić, Aleksandar Z.
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Kojić, Milan
AU  - Gašić, Uroš
AU  - Barac, Miroljub B.
AU  - Stanojević, Sladana P.
AU  - Popović, Dusanka A.
AU  - Banjac, Nebojša R.
AU  - Tesić, Zivoslav Lj.
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1274
AB  - The aim of this study was to evaluate the effect of enriching a complex food matrix (FM) with grape extracts on polyphenol content, composition, bioaccessibility and antioxidant activity during digestion. The grape extracts and FM were separately tested under the same conditions as controls. The FM by itself contains a significant amount of phenolic acids and flavonols, influencing the final recovery of polyphenols from grape extracts. The FM significantly increased the total recovery of polyphenols after digestion of grape seed extracts compared to those digested without the FM; however, a low recovery of proantocyanidins and total flavonoids was observed. Digestive fluids and FM compounds significantly increased the total polyphenol content of grape digests and significantly contributed to their ABTS(center dot+) scavenging activity and ferrous-ion-chelating capacity. The present study suggested that enrichment of meat-and cereal-based products with grape polyphenol extracts could be a good strategy to formulate a healthier diet.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?
EP  - 44
SP  - 28
VL  - 284
DO  - 10.1016/j.foodchem.2019.01.107
ER  - 

@article{
author = "Pesić, Mirjana B. and Milincić, Danijel D. and Kostić, Aleksandar Z. and Stanisavljević, Nemanja and Vukotić, Goran and Kojić, Milan and Gašić, Uroš and Barac, Miroljub B. and Stanojević, Sladana P. and Popović, Dusanka A. and Banjac, Nebojša R. and Tesić, Zivoslav Lj.",
year = "2019",
abstract = "The aim of this study was to evaluate the effect of enriching a complex food matrix (FM) with grape extracts on polyphenol content, composition, bioaccessibility and antioxidant activity during digestion. The grape extracts and FM were separately tested under the same conditions as controls. The FM by itself contains a significant amount of phenolic acids and flavonols, influencing the final recovery of polyphenols from grape extracts. The FM significantly increased the total recovery of polyphenols after digestion of grape seed extracts compared to those digested without the FM; however, a low recovery of proantocyanidins and total flavonoids was observed. Digestive fluids and FM compounds significantly increased the total polyphenol content of grape digests and significantly contributed to their ABTS(center dot+) scavenging activity and ferrous-ion-chelating capacity. The present study suggested that enrichment of meat-and cereal-based products with grape polyphenol extracts could be a good strategy to formulate a healthier diet.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?",
pages = "44-28",
volume = "284",
doi = "10.1016/j.foodchem.2019.01.107"
}

Pesić, M. B., Milincić, D. D., Kostić, A. Z., Stanisavljević, N., Vukotić, G., Kojić, M., Gašić, U., Barac, M. B., Stanojević, S. P., Popović, D. A., Banjac, N. R.,& Tesić, Z. Lj.. (2019). In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?. in Food Chemistry
Elsevier Sci Ltd, Oxford., 284, 28-44.
https://doi.org/10.1016/j.foodchem.2019.01.107

Pesić MB, Milincić DD, Kostić AZ, Stanisavljević N, Vukotić G, Kojić M, Gašić U, Barac MB, Stanojević SP, Popović DA, Banjac NR, Tesić ZL. In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?. in Food Chemistry. 2019;284:28-44.
doi:10.1016/j.foodchem.2019.01.107 .

Pesić, Mirjana B., Milincić, Danijel D., Kostić, Aleksandar Z., Stanisavljević, Nemanja, Vukotić, Goran, Kojić, Milan, Gašić, Uroš, Barac, Miroljub B., Stanojević, Sladana P., Popović, Dusanka A., Banjac, Nebojša R., Tesić, Zivoslav Lj., "In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?" in Food Chemistry, 284 (2019):28-44,
https://doi.org/10.1016/j.foodchem.2019.01.107 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Vukotić, Goran
AU  - Stanisavljević, Nemanja
AU  - Kosanović, Dejana
AU  - Molnar, Zsuzsanna
AU  - Begović, Jelena
AU  - Terzić-Vidojević, Amarela
AU  - Jeney, Galina
AU  - Ljubobratović, Uros
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1207
AB  - The aim of this research was to improve nutritive value of fishmeal-based feed by lactobacilli in order to achieve satisfactory nutrient availability needed to support fish development. Feed was solid-state treated at a laboratory scale with the combination of Lactobacillus paracaseisubsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 in different experimental settings, which included the variation of strain ratio, total lactobacilli concentration, percentage of moisture and duration of incubation. Short peptides, soluble proteins, phospho-, neutral and unsaturated lipids were quantified. Differences among treated and control feeds were evaluated by Student t-test, while Gaussian process regression (GPR) modeling was employed to simulate the incubation process and define the optimal treatment combination in the context of overall feed nutritional profile. Treatment duration was shown to be the critical determinant of final outcome, either as single factor or via interaction with strain ratio. Optimal nutrient balance was achieved with 12 h incubation period, 260% moisture, 75:25 and 50:50 BGHN14:BGT10 ratios and 200 mg of lactobacilli per g of dry feed. This study should serve as the basis for large-scale tests which would simulate on-farm production of both fishmeal-based and unconventional, lower cost aquafeed with added value.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed
IS  - 7
VL  - 14
DO  - 10.1371/journal.pone.0219558
ER  - 

@article{
author = "Lukić, Jovanka and Vukotić, Goran and Stanisavljević, Nemanja and Kosanović, Dejana and Molnar, Zsuzsanna and Begović, Jelena and Terzić-Vidojević, Amarela and Jeney, Galina and Ljubobratović, Uros",
year = "2019",
abstract = "The aim of this research was to improve nutritive value of fishmeal-based feed by lactobacilli in order to achieve satisfactory nutrient availability needed to support fish development. Feed was solid-state treated at a laboratory scale with the combination of Lactobacillus paracaseisubsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 in different experimental settings, which included the variation of strain ratio, total lactobacilli concentration, percentage of moisture and duration of incubation. Short peptides, soluble proteins, phospho-, neutral and unsaturated lipids were quantified. Differences among treated and control feeds were evaluated by Student t-test, while Gaussian process regression (GPR) modeling was employed to simulate the incubation process and define the optimal treatment combination in the context of overall feed nutritional profile. Treatment duration was shown to be the critical determinant of final outcome, either as single factor or via interaction with strain ratio. Optimal nutrient balance was achieved with 12 h incubation period, 260% moisture, 75:25 and 50:50 BGHN14:BGT10 ratios and 200 mg of lactobacilli per g of dry feed. This study should serve as the basis for large-scale tests which would simulate on-farm production of both fishmeal-based and unconventional, lower cost aquafeed with added value.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed",
number = "7",
volume = "14",
doi = "10.1371/journal.pone.0219558"
}

Lukić, J., Vukotić, G., Stanisavljević, N., Kosanović, D., Molnar, Z., Begović, J., Terzić-Vidojević, A., Jeney, G.,& Ljubobratović, U.. (2019). Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed. in PLoS One
Public Library Science, San Francisco., 14(7).
https://doi.org/10.1371/journal.pone.0219558

Lukić J, Vukotić G, Stanisavljević N, Kosanović D, Molnar Z, Begović J, Terzić-Vidojević A, Jeney G, Ljubobratović U. Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed. in PLoS One. 2019;14(7).
doi:10.1371/journal.pone.0219558 .

Lukić, Jovanka, Vukotić, Goran, Stanisavljević, Nemanja, Kosanović, Dejana, Molnar, Zsuzsanna, Begović, Jelena, Terzić-Vidojević, Amarela, Jeney, Galina, Ljubobratović, Uros, "Solid state treatment with Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 improves nutrient bioavailability in granular fish feed" in PLoS One, 14, no. 7 (2019),
https://doi.org/10.1371/journal.pone.0219558 . .

TY  - JOUR
AU  - Vukotić, Goran
AU  - Polović, Natalija
AU  - Mirković, Nemanja
AU  - Jovčić, Branko
AU  - Stanisavljević, Nemanja
AU  - Fira, Đorđe
AU  - Kojić, Milan
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1251
AB  - In our previous study we demonstrated that proteinase PrtP is able to impair bacteriocin LcnB activity, despite being produced by the same organism and encoded by the same plasmid. However, precise mechanism of this action, i.e., the exact cleavage site within LcnB bacteriocin, as well as its effect on antimicrobial activity of the resulting peptide remained vague. Here we further explored the interplay between these two proteins and defined, using mass spectrometry, that this unusual hydrolysis indeed occurs in vivo, between the sixth and seventh amino acid on the N terminus of LcnB. To address whether the cleaved form of LcnB retains any level of activity, both recombinant and chemically synthesized variant of truncated LcnB were engineered and produced, but demonstrated no antimicrobial activity. When LcnB was recombinantly overexpressed and subjected to PrtP digestion, the change in its antimicrobial activity was monitored and the degradation products analyzed with reverse-phase high-pressure liquid chromatography. The results confirmed the inactivity of the truncated LcnB and additionally corroborated the PrtP cleavage site in LcnB bacteriocin. In addition, it was demonstrated that, once truncated, LcnB is not able to bind its receptor and is susceptible to additional hydrolysis. This is the first report on proteolytic inactivation of bacteriocins inside the same bacterial host.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. lactis BGMN1-501
VL  - 10
DO  - 10.3389/fmicb.2019.00874
ER  - 

@article{
author = "Vukotić, Goran and Polović, Natalija and Mirković, Nemanja and Jovčić, Branko and Stanisavljević, Nemanja and Fira, Đorđe and Kojić, Milan",
year = "2019",
abstract = "In our previous study we demonstrated that proteinase PrtP is able to impair bacteriocin LcnB activity, despite being produced by the same organism and encoded by the same plasmid. However, precise mechanism of this action, i.e., the exact cleavage site within LcnB bacteriocin, as well as its effect on antimicrobial activity of the resulting peptide remained vague. Here we further explored the interplay between these two proteins and defined, using mass spectrometry, that this unusual hydrolysis indeed occurs in vivo, between the sixth and seventh amino acid on the N terminus of LcnB. To address whether the cleaved form of LcnB retains any level of activity, both recombinant and chemically synthesized variant of truncated LcnB were engineered and produced, but demonstrated no antimicrobial activity. When LcnB was recombinantly overexpressed and subjected to PrtP digestion, the change in its antimicrobial activity was monitored and the degradation products analyzed with reverse-phase high-pressure liquid chromatography. The results confirmed the inactivity of the truncated LcnB and additionally corroborated the PrtP cleavage site in LcnB bacteriocin. In addition, it was demonstrated that, once truncated, LcnB is not able to bind its receptor and is susceptible to additional hydrolysis. This is the first report on proteolytic inactivation of bacteriocins inside the same bacterial host.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. lactis BGMN1-501",
volume = "10",
doi = "10.3389/fmicb.2019.00874"
}

Vukotić, G., Polović, N., Mirković, N., Jovčić, B., Stanisavljević, N., Fira, Đ.,& Kojić, M.. (2019). Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. lactis BGMN1-501. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fmicb.2019.00874

Vukotić G, Polović N, Mirković N, Jovčić B, Stanisavljević N, Fira Đ, Kojić M. Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. lactis BGMN1-501. in Frontiers in Microbiology. 2019;10.
doi:10.3389/fmicb.2019.00874 .

Vukotić, Goran, Polović, Natalija, Mirković, Nemanja, Jovčić, Branko, Stanisavljević, Nemanja, Fira, Đorđe, Kojić, Milan, "Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. lactis BGMN1-501" in Frontiers in Microbiology, 10 (2019),
https://doi.org/10.3389/fmicb.2019.00874 . .

TY  - JOUR
AU  - Pesić, Mirjana B.
AU  - Milincić, Danijel D.
AU  - Kostić, Aleksandar Z.
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Kojić, Milan
AU  - Gašić, Uroš
AU  - Barac, Miroljub B.
AU  - Stanojević, Sladana P.
AU  - Popović, Dusanka A.
AU  - Banjac, Nebojša R.
AU  - Tesić, Zivoslav Lj.
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1609
AB  - The aim of this study was to evaluate the effect of enriching a complex food matrix (FM) with grape extracts on polyphenol content, composition, bioaccessibility and antioxidant activity during digestion. The grape extracts and FM were separately tested under the same conditions as controls. The FM by itself contains a significant amount of phenolic acids and flavonols, influencing the final recovery of polyphenols from grape extracts. The FM significantly increased the total recovery of polyphenols after digestion of grape seed extracts compared to those digested without the FM; however, a low recovery of proantocyanidins and total flavonoids was observed. Digestive fluids and FM compounds significantly increased the total polyphenol content of grape digests and significantly contributed to their ABTS(center dot+) scavenging activity and ferrous-ion-chelating capacity. The present study suggested that enrichment of meat-and cereal-based products with grape polyphenol extracts could be a good strategy to formulate a healthier diet.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?
EP  - 44
SP  - 28
VL  - 284
DO  - 10.1016/j.foodchem.2019.01.107
ER  - 

@article{
author = "Pesić, Mirjana B. and Milincić, Danijel D. and Kostić, Aleksandar Z. and Stanisavljević, Nemanja and Vukotić, Goran and Kojić, Milan and Gašić, Uroš and Barac, Miroljub B. and Stanojević, Sladana P. and Popović, Dusanka A. and Banjac, Nebojša R. and Tesić, Zivoslav Lj.",
year = "2019",
abstract = "The aim of this study was to evaluate the effect of enriching a complex food matrix (FM) with grape extracts on polyphenol content, composition, bioaccessibility and antioxidant activity during digestion. The grape extracts and FM were separately tested under the same conditions as controls. The FM by itself contains a significant amount of phenolic acids and flavonols, influencing the final recovery of polyphenols from grape extracts. The FM significantly increased the total recovery of polyphenols after digestion of grape seed extracts compared to those digested without the FM; however, a low recovery of proantocyanidins and total flavonoids was observed. Digestive fluids and FM compounds significantly increased the total polyphenol content of grape digests and significantly contributed to their ABTS(center dot+) scavenging activity and ferrous-ion-chelating capacity. The present study suggested that enrichment of meat-and cereal-based products with grape polyphenol extracts could be a good strategy to formulate a healthier diet.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?",
pages = "44-28",
volume = "284",
doi = "10.1016/j.foodchem.2019.01.107"
}

Pesić, M. B., Milincić, D. D., Kostić, A. Z., Stanisavljević, N., Vukotić, G., Kojić, M., Gašić, U., Barac, M. B., Stanojević, S. P., Popović, D. A., Banjac, N. R.,& Tesić, Z. Lj.. (2019). In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?. in Food Chemistry
Elsevier Sci Ltd, Oxford., 284, 28-44.
https://doi.org/10.1016/j.foodchem.2019.01.107

Pesić MB, Milincić DD, Kostić AZ, Stanisavljević N, Vukotić G, Kojić M, Gašić U, Barac MB, Stanojević SP, Popović DA, Banjac NR, Tesić ZL. In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?. in Food Chemistry. 2019;284:28-44.
doi:10.1016/j.foodchem.2019.01.107 .

Pesić, Mirjana B., Milincić, Danijel D., Kostić, Aleksandar Z., Stanisavljević, Nemanja, Vukotić, Goran, Kojić, Milan, Gašić, Uroš, Barac, Miroljub B., Stanojević, Sladana P., Popović, Dusanka A., Banjac, Nebojša R., Tesić, Zivoslav Lj., "In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?" in Food Chemistry, 284 (2019):28-44,
https://doi.org/10.1016/j.foodchem.2019.01.107 . .

TY  - JOUR
AU  - Miljković, Marija
AU  - Malešević, Milka
AU  - Filipić, Brankica
AU  - Vukotić, Goran
AU  - Kojić, Milan
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1156
AB  - Restriction enzymes are the main defence system against foreign DNA, in charge of preserving genome integrity. Lactococcus raffinolactis BGTRK10-1 expresses LraI Type II restriction-modification enzyme, whose activity is similar to that shown for EcoRI; LraI methyltransferase protects DNA from EcoRI cleavage. The gene encoding LraI endonuclease was cloned and overexpressed in E. coli. Purified enzyme showed the highest specific activity at lower temperatures (between 13 degrees C and 37 degrees C) and was stable after storage at -20 degrees C in 50% glycerol. The concentration of monovalent ions in the reaction buffer required for optimal activity of LraI restriction enzyme was 100 mM or higher. The recognition and cleavage sequence for LraI restriction enzyme was determined as 5'-G/AATTC-3', indicating that LraI restriction enzyme is an isoschizomer of EcoRI. In the reaction buffer with a lower salt concentration, LraI exhibits star activity and specifically recognizes and cuts another alternative sequence 5'-A/AATTC-3', leaving the same sticky ends on fragments as EcoRI, which makes them clonable into a linearized vector. Phylogenetic analysis based on sequence alignment pointed out the common origin of LraI restriction-modification system with previously described EcoRI-like restriction-modification systems.
PB  - Hindawi Ltd, London
T2  - Biomed Research International
T1  - LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
VL  - 2018
DO  - 10.1155/2018/5657085
ER  - 

@article{
author = "Miljković, Marija and Malešević, Milka and Filipić, Brankica and Vukotić, Goran and Kojić, Milan",
year = "2018",
abstract = "Restriction enzymes are the main defence system against foreign DNA, in charge of preserving genome integrity. Lactococcus raffinolactis BGTRK10-1 expresses LraI Type II restriction-modification enzyme, whose activity is similar to that shown for EcoRI; LraI methyltransferase protects DNA from EcoRI cleavage. The gene encoding LraI endonuclease was cloned and overexpressed in E. coli. Purified enzyme showed the highest specific activity at lower temperatures (between 13 degrees C and 37 degrees C) and was stable after storage at -20 degrees C in 50% glycerol. The concentration of monovalent ions in the reaction buffer required for optimal activity of LraI restriction enzyme was 100 mM or higher. The recognition and cleavage sequence for LraI restriction enzyme was determined as 5'-G/AATTC-3', indicating that LraI restriction enzyme is an isoschizomer of EcoRI. In the reaction buffer with a lower salt concentration, LraI exhibits star activity and specifically recognizes and cuts another alternative sequence 5'-A/AATTC-3', leaving the same sticky ends on fragments as EcoRI, which makes them clonable into a linearized vector. Phylogenetic analysis based on sequence alignment pointed out the common origin of LraI restriction-modification system with previously described EcoRI-like restriction-modification systems.",
publisher = "Hindawi Ltd, London",
journal = "Biomed Research International",
title = "LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity",
volume = "2018",
doi = "10.1155/2018/5657085"
}

Miljković, M., Malešević, M., Filipić, B., Vukotić, G.,& Kojić, M.. (2018). LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity. in Biomed Research International
Hindawi Ltd, London., 2018.
https://doi.org/10.1155/2018/5657085

Miljković M, Malešević M, Filipić B, Vukotić G, Kojić M. LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity. in Biomed Research International. 2018;2018.
doi:10.1155/2018/5657085 .

Miljković, Marija, Malešević, Milka, Filipić, Brankica, Vukotić, Goran, Kojić, Milan, "LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity" in Biomed Research International, 2018 (2018),
https://doi.org/10.1155/2018/5657085 . .

TY  - JOUR
AU  - Ljubobratović, Uros
AU  - Kosanović, Dejana
AU  - Vukotić, Goran
AU  - Molnar, Zsuzsanna
AU  - Stanisavljević, Nemanja
AU  - Ristović, Tijana
AU  - Peter, Geza
AU  - Lukić, Jovanka
AU  - Jeney, Galina
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1026
AB  - This research aimed to test the effects of lactobacilli, applied to cultured pike-perch, either through hydrolyzed OTOHIME fish diet, or through Artemia nauplii, on fish growth, microbiota balance and skeletal development. On the 12th Day Post Hatching (DPH) fish were divided into following treatment groups: two groups received the combination of OTOHIME and nauplii enriched either with Lactobacillus paracasei BGHN14 + Lactobacillus rhamnosus BGTH or with Lactobacillus reuteri BGGO6-55 + Lactobacillus salivarius BGHO1, and one group received OTOHIME hydrolyzed by BGHN14 + BGT10 and non-enriched nauplii. Control group received non-enriched nauplii and non-hydrolyzed OTOHIME. The treatment lasted 14 days and fish were sacrificed on the 26th DPH for the assessment of digestive enzyme activity and microbiota composition. Individual total lengths and individual body weights were recorded at the end of the treatments, on the 26th DPH, and also on the 45th DPH, in parallel with the evaluation of skeletal deformities and fish survival. Our results indicated positive effect of Anemia enriched with BGGO6-55 + BGHO1 on fish growth, skeletal development and trypsin to chymotrypsin activity ratio (T/C), as an indicator of protein digestibility. Hydrolysis of OTOHIME was also associated with better skeletal development, higher T/C values and lower levels of Aeromonas and Mycobacterium spp., which are important fish pathogens. Though additional testing in larger cohort studies is needed, these observations are promising in terms of usage of probiotics for improved environmentally friendly production of pike-perch in Recirculating Aquaculture System (RAS).
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): A pilot study
EP  - 462
SP  - 451
VL  - 115
DO  - 10.1016/j.rvsc.2017.07.018
ER  - 

@article{
author = "Ljubobratović, Uros and Kosanović, Dejana and Vukotić, Goran and Molnar, Zsuzsanna and Stanisavljević, Nemanja and Ristović, Tijana and Peter, Geza and Lukić, Jovanka and Jeney, Galina",
year = "2017",
abstract = "This research aimed to test the effects of lactobacilli, applied to cultured pike-perch, either through hydrolyzed OTOHIME fish diet, or through Artemia nauplii, on fish growth, microbiota balance and skeletal development. On the 12th Day Post Hatching (DPH) fish were divided into following treatment groups: two groups received the combination of OTOHIME and nauplii enriched either with Lactobacillus paracasei BGHN14 + Lactobacillus rhamnosus BGTH or with Lactobacillus reuteri BGGO6-55 + Lactobacillus salivarius BGHO1, and one group received OTOHIME hydrolyzed by BGHN14 + BGT10 and non-enriched nauplii. Control group received non-enriched nauplii and non-hydrolyzed OTOHIME. The treatment lasted 14 days and fish were sacrificed on the 26th DPH for the assessment of digestive enzyme activity and microbiota composition. Individual total lengths and individual body weights were recorded at the end of the treatments, on the 26th DPH, and also on the 45th DPH, in parallel with the evaluation of skeletal deformities and fish survival. Our results indicated positive effect of Anemia enriched with BGGO6-55 + BGHO1 on fish growth, skeletal development and trypsin to chymotrypsin activity ratio (T/C), as an indicator of protein digestibility. Hydrolysis of OTOHIME was also associated with better skeletal development, higher T/C values and lower levels of Aeromonas and Mycobacterium spp., which are important fish pathogens. Though additional testing in larger cohort studies is needed, these observations are promising in terms of usage of probiotics for improved environmentally friendly production of pike-perch in Recirculating Aquaculture System (RAS).",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): A pilot study",
pages = "462-451",
volume = "115",
doi = "10.1016/j.rvsc.2017.07.018"
}

Ljubobratović, U., Kosanović, D., Vukotić, G., Molnar, Z., Stanisavljević, N., Ristović, T., Peter, G., Lukić, J.,& Jeney, G.. (2017). Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): A pilot study. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 115, 451-462.
https://doi.org/10.1016/j.rvsc.2017.07.018

Ljubobratović U, Kosanović D, Vukotić G, Molnar Z, Stanisavljević N, Ristović T, Peter G, Lukić J, Jeney G. Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): A pilot study. in Research in Veterinary Science. 2017;115:451-462.
doi:10.1016/j.rvsc.2017.07.018 .

Ljubobratović, Uros, Kosanović, Dejana, Vukotić, Goran, Molnar, Zsuzsanna, Stanisavljević, Nemanja, Ristović, Tijana, Peter, Geza, Lukić, Jovanka, Jeney, Galina, "Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): A pilot study" in Research in Veterinary Science, 115 (2017):451-462,
https://doi.org/10.1016/j.rvsc.2017.07.018 . .

TY  - JOUR
AU  - Vukotić, Goran
AU  - Strahinić, Ivana
AU  - Begović, Jelena
AU  - Lukić, Jovanka
AU  - Kojić, Milan
AU  - Fira, Đorđe
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/992
AB  - Lactocepins or CEPs are large cell wall bound extracellular proteinases of lactic acid bacteria, involved in protein breakdown and utilization. They are responsible for many health-promoting traits of food products fermented with these organisms, but also essential for probiotic effects of certain strains. Different mesophilic strains selected within the species Lactobacillus zeae, Lb. casei, Lb. rhamnosus, and Lb. plantarum were analyzed for their proteolytic activity towards main fractions of milk proteins-caseins and whey proteins. The strains showing excellent proteolytic features were further examined for presence of corresponding proteinase gene(s). It was found that Lb. zeae LMG17315 possessed catalytic domains of three distinct proteinase genes, unique feature in Lb. casei group, which are similar but not identical to previously characterized prtP and prtR genes. Lb. casei neotype strain ATCC393 was also analysed and based on obtained results its reclassification in taxon Lb. zeae is supported. In addition, we report catalytic domain of prtR-type gene in Lb. plantarum LMG9208, which is first such report in this species, and it is first time that this gene is reported outside Lb. casei group.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Microbiology
T1  - Survey on proteolytic activity and diversity of proteinase genes in mesophilic lactobacilli
EP  - 41
IS  - 1
SP  - 33
VL  - 85
DO  - 10.1134/S002626171601015X
ER  - 

@article{
author = "Vukotić, Goran and Strahinić, Ivana and Begović, Jelena and Lukić, Jovanka and Kojić, Milan and Fira, Đorđe",
year = "2016",
abstract = "Lactocepins or CEPs are large cell wall bound extracellular proteinases of lactic acid bacteria, involved in protein breakdown and utilization. They are responsible for many health-promoting traits of food products fermented with these organisms, but also essential for probiotic effects of certain strains. Different mesophilic strains selected within the species Lactobacillus zeae, Lb. casei, Lb. rhamnosus, and Lb. plantarum were analyzed for their proteolytic activity towards main fractions of milk proteins-caseins and whey proteins. The strains showing excellent proteolytic features were further examined for presence of corresponding proteinase gene(s). It was found that Lb. zeae LMG17315 possessed catalytic domains of three distinct proteinase genes, unique feature in Lb. casei group, which are similar but not identical to previously characterized prtP and prtR genes. Lb. casei neotype strain ATCC393 was also analysed and based on obtained results its reclassification in taxon Lb. zeae is supported. In addition, we report catalytic domain of prtR-type gene in Lb. plantarum LMG9208, which is first such report in this species, and it is first time that this gene is reported outside Lb. casei group.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Microbiology",
title = "Survey on proteolytic activity and diversity of proteinase genes in mesophilic lactobacilli",
pages = "41-33",
number = "1",
volume = "85",
doi = "10.1134/S002626171601015X"
}

Vukotić, G., Strahinić, I., Begović, J., Lukić, J., Kojić, M.,& Fira, Đ.. (2016). Survey on proteolytic activity and diversity of proteinase genes in mesophilic lactobacilli. in Microbiology
Maik Nauka/Interperiodica/Springer, New York., 85(1), 33-41.
https://doi.org/10.1134/S002626171601015X

Vukotić G, Strahinić I, Begović J, Lukić J, Kojić M, Fira Đ. Survey on proteolytic activity and diversity of proteinase genes in mesophilic lactobacilli. in Microbiology. 2016;85(1):33-41.
doi:10.1134/S002626171601015X .

Vukotić, Goran, Strahinić, Ivana, Begović, Jelena, Lukić, Jovanka, Kojić, Milan, Fira, Đorđe, "Survey on proteolytic activity and diversity of proteinase genes in mesophilic lactobacilli" in Microbiology, 85, no. 1 (2016):33-41,
https://doi.org/10.1134/S002626171601015X . .

TY  - JOUR
AU  - Vukotić, Goran
AU  - Matić, Ivana
AU  - Begović, Jelena
AU  - Besu, Irina
AU  - Kojić, Milan
AU  - Đokić, Jelena
AU  - Juranić, Zorica
AU  - Strahinić, Ivana
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/928
AB  - The level of humoral immunoreactivity to total cows' milk proteins (TCMP) in sera of patients suffering from recurrent oral ulcerations, gastrointestinal diseases or haematological malignancies was investigated. TCMP were also hydrolysed with two different species of lactobacilli and dramatic changes in the levels of specific IgG and IgE were found with statistically significant decreases in the levels of specific antibodies in sera from all patient groups. The levels below cut-off values of IgG specific for TCMP hydrolysates were detected in sera from all patients, while values of IgE for hydrolysates obtained with Lactobacillus helveticus BGRA43 and Lactobacillus zeae LMG17315were below cut-off in 85% and 97% of patients, respectively. Competitive ELISA confirmed the specificity of antibodies for immunogenic TCMP epitopes, demonstrating that lactobacilli hydrolyse TCMP by degrading immunogenic epitopes, and could therefore be used in processing of milk proteins to obtain products suitable for patients with altered immune response on TCMP.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Dairy Journal
T1  - Lactobacilli hydrolysis of cows' milk proteins abrogates their humoral immunoreactivity in patients with immune-mediated diseases
EP  - 7
SP  - 1
VL  - 63
DO  - 10.1016/j.idairyj.2016.07.009
ER  - 

@article{
author = "Vukotić, Goran and Matić, Ivana and Begović, Jelena and Besu, Irina and Kojić, Milan and Đokić, Jelena and Juranić, Zorica and Strahinić, Ivana",
year = "2016",
abstract = "The level of humoral immunoreactivity to total cows' milk proteins (TCMP) in sera of patients suffering from recurrent oral ulcerations, gastrointestinal diseases or haematological malignancies was investigated. TCMP were also hydrolysed with two different species of lactobacilli and dramatic changes in the levels of specific IgG and IgE were found with statistically significant decreases in the levels of specific antibodies in sera from all patient groups. The levels below cut-off values of IgG specific for TCMP hydrolysates were detected in sera from all patients, while values of IgE for hydrolysates obtained with Lactobacillus helveticus BGRA43 and Lactobacillus zeae LMG17315were below cut-off in 85% and 97% of patients, respectively. Competitive ELISA confirmed the specificity of antibodies for immunogenic TCMP epitopes, demonstrating that lactobacilli hydrolyse TCMP by degrading immunogenic epitopes, and could therefore be used in processing of milk proteins to obtain products suitable for patients with altered immune response on TCMP.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Dairy Journal",
title = "Lactobacilli hydrolysis of cows' milk proteins abrogates their humoral immunoreactivity in patients with immune-mediated diseases",
pages = "7-1",
volume = "63",
doi = "10.1016/j.idairyj.2016.07.009"
}

Vukotić, G., Matić, I., Begović, J., Besu, I., Kojić, M., Đokić, J., Juranić, Z.,& Strahinić, I.. (2016). Lactobacilli hydrolysis of cows' milk proteins abrogates their humoral immunoreactivity in patients with immune-mediated diseases. in International Dairy Journal
Elsevier Sci Ltd, Oxford., 63, 1-7.
https://doi.org/10.1016/j.idairyj.2016.07.009

Vukotić G, Matić I, Begović J, Besu I, Kojić M, Đokić J, Juranić Z, Strahinić I. Lactobacilli hydrolysis of cows' milk proteins abrogates their humoral immunoreactivity in patients with immune-mediated diseases. in International Dairy Journal. 2016;63:1-7.
doi:10.1016/j.idairyj.2016.07.009 .

Vukotić, Goran, Matić, Ivana, Begović, Jelena, Besu, Irina, Kojić, Milan, Đokić, Jelena, Juranić, Zorica, Strahinić, Ivana, "Lactobacilli hydrolysis of cows' milk proteins abrogates their humoral immunoreactivity in patients with immune-mediated diseases" in International Dairy Journal, 63 (2016):1-7,
https://doi.org/10.1016/j.idairyj.2016.07.009 . .

TY  - JOUR
AU  - Mirković, Nemanja
AU  - Polović, Natalija
AU  - Vukotić, Goran
AU  - Jovčić, Branko
AU  - Miljković, Marija
AU  - Radulović, Zorica
AU  - Diep, Dzung B.
AU  - Kojić, Milan
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/980
AB  - Bacteriocin producers normally possess dedicated immunity systems to protect themselves from their own bacteriocins. Lactococcus lactis strains LMG2081 and BGBM50 are known as lactococcin G producers. However, BGBM50 was sensitive to LMG2081, which indicated that LMG2081 might produce additional bacteriocins that are not present in BGBM50. Therefore, whole-genome sequencing of the two strains was performed, and a lantibiotic operon (called lctLMG) was identified in LMG2081 but not in BGBM50. The lctLMG operon contains six open reading frames; the first three genes, lmgA, lmgM, and lmgT, are involved in the biosynthesis and export of bacteriocin, while the other three genes, lmgF, lmgE, and lmgG, are involved in lantibiotic immunity. Mutational analysis confirmed that the lctLMG operon is responsible for the additional antimicrobial activity. Specifically, site-directed mutation within this operon rendered LMG2081 inactive toward BGBM50. Subsequent purification and electrospray ionization-time of flight mass spectrometric analysis confirmed that the lantibiotic bacteriocin called lacticin LMG is exported as a 25-amino-acid peptide. Lacticin LMG is highly similar to the lacticin 481 group. It is interesting that a bacteriocin producer produces two different classes of bacteriocins, whose operons are located in the chromosome and a plasmid.
PB  - Amer Soc Microbiology, Washington
T2  - Applied and Environmental Microbiology
T1  - Lactococcus lactis LMG2081 Produces Two Bacteriocins, a Nonlantibiotic and a Novel Lantibiotic
EP  - 2562
IS  - 8
SP  - 2555
VL  - 82
DO  - 10.1128/AEM.03988-15
ER  - 

@article{
author = "Mirković, Nemanja and Polović, Natalija and Vukotić, Goran and Jovčić, Branko and Miljković, Marija and Radulović, Zorica and Diep, Dzung B. and Kojić, Milan",
year = "2016",
abstract = "Bacteriocin producers normally possess dedicated immunity systems to protect themselves from their own bacteriocins. Lactococcus lactis strains LMG2081 and BGBM50 are known as lactococcin G producers. However, BGBM50 was sensitive to LMG2081, which indicated that LMG2081 might produce additional bacteriocins that are not present in BGBM50. Therefore, whole-genome sequencing of the two strains was performed, and a lantibiotic operon (called lctLMG) was identified in LMG2081 but not in BGBM50. The lctLMG operon contains six open reading frames; the first three genes, lmgA, lmgM, and lmgT, are involved in the biosynthesis and export of bacteriocin, while the other three genes, lmgF, lmgE, and lmgG, are involved in lantibiotic immunity. Mutational analysis confirmed that the lctLMG operon is responsible for the additional antimicrobial activity. Specifically, site-directed mutation within this operon rendered LMG2081 inactive toward BGBM50. Subsequent purification and electrospray ionization-time of flight mass spectrometric analysis confirmed that the lantibiotic bacteriocin called lacticin LMG is exported as a 25-amino-acid peptide. Lacticin LMG is highly similar to the lacticin 481 group. It is interesting that a bacteriocin producer produces two different classes of bacteriocins, whose operons are located in the chromosome and a plasmid.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Applied and Environmental Microbiology",
title = "Lactococcus lactis LMG2081 Produces Two Bacteriocins, a Nonlantibiotic and a Novel Lantibiotic",
pages = "2562-2555",
number = "8",
volume = "82",
doi = "10.1128/AEM.03988-15"
}

Mirković, N., Polović, N., Vukotić, G., Jovčić, B., Miljković, M., Radulović, Z., Diep, D. B.,& Kojić, M.. (2016). Lactococcus lactis LMG2081 Produces Two Bacteriocins, a Nonlantibiotic and a Novel Lantibiotic. in Applied and Environmental Microbiology
Amer Soc Microbiology, Washington., 82(8), 2555-2562.
https://doi.org/10.1128/AEM.03988-15

Mirković N, Polović N, Vukotić G, Jovčić B, Miljković M, Radulović Z, Diep DB, Kojić M. Lactococcus lactis LMG2081 Produces Two Bacteriocins, a Nonlantibiotic and a Novel Lantibiotic. in Applied and Environmental Microbiology. 2016;82(8):2555-2562.
doi:10.1128/AEM.03988-15 .

Mirković, Nemanja, Polović, Natalija, Vukotić, Goran, Jovčić, Branko, Miljković, Marija, Radulović, Zorica, Diep, Dzung B., Kojić, Milan, "Lactococcus lactis LMG2081 Produces Two Bacteriocins, a Nonlantibiotic and a Novel Lantibiotic" in Applied and Environmental Microbiology, 82, no. 8 (2016):2555-2562,
https://doi.org/10.1128/AEM.03988-15 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran 
AU  - Pastor, Ferenc T.
AU  - Suznjević, Desanka
AU  - Jovanović, Živko
AU  - Strahinić, Ivana
AU  - Fira, Đorđe
AU  - Radović, Svetlana S.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/845
AB  - Nine Lactobacillus strains known for surface proteinase activity were chosen from our collection and tested for their ability to grow in pea seed protein-based medium, and to hydrolyze purified pea proteins in order to produce peptides with antioxidant (AO) activity. Two strains, Lactobacillus rhamnosus BGT10 and Lactobacillus zeae LMG17315, exhibited strong proteolytic activity against pea proteins. The AO activity of the pea hydrolysate fraction, MW  lt  10 kDa, obtained by the fermentation of purified pea proteins with Lactobacillus rhamnosus BGT10, was tested by standard spectrophotometric assays (DPPH, ABTS, Fe3+-reducing capacity) and the recently developed direct current (DC) polarographic assay. The low molecular weight fraction of the obtained hydrolysate was separated using ion exchange chromatography, while the AO activity of eluted fractions was determined by means of a sensitive DC polarographic assay without previous concentration of samples. Results revealed that the fraction present in low abundance that contained basic peptides possessed the highest antioxidant activity. Based on the obtained results, it can be concluded that Lactobacillus rhamnosus BGT10 should be further investigated as a candidate strain for large-scale production of bioactive peptides from legume proteins.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria
EP  - 1042
IS  - 3
SP  - 1033
VL  - 67
DO  - 10.2298/ABS150130066S
ER  - 

@article{
author = "Stanisavljević, Nemanja and Vukotić, Goran  and Pastor, Ferenc T. and Suznjević, Desanka and Jovanović, Živko and Strahinić, Ivana and Fira, Đorđe and Radović, Svetlana S.",
year = "2015",
abstract = "Nine Lactobacillus strains known for surface proteinase activity were chosen from our collection and tested for their ability to grow in pea seed protein-based medium, and to hydrolyze purified pea proteins in order to produce peptides with antioxidant (AO) activity. Two strains, Lactobacillus rhamnosus BGT10 and Lactobacillus zeae LMG17315, exhibited strong proteolytic activity against pea proteins. The AO activity of the pea hydrolysate fraction, MW  lt  10 kDa, obtained by the fermentation of purified pea proteins with Lactobacillus rhamnosus BGT10, was tested by standard spectrophotometric assays (DPPH, ABTS, Fe3+-reducing capacity) and the recently developed direct current (DC) polarographic assay. The low molecular weight fraction of the obtained hydrolysate was separated using ion exchange chromatography, while the AO activity of eluted fractions was determined by means of a sensitive DC polarographic assay without previous concentration of samples. Results revealed that the fraction present in low abundance that contained basic peptides possessed the highest antioxidant activity. Based on the obtained results, it can be concluded that Lactobacillus rhamnosus BGT10 should be further investigated as a candidate strain for large-scale production of bioactive peptides from legume proteins.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria",
pages = "1042-1033",
number = "3",
volume = "67",
doi = "10.2298/ABS150130066S"
}

Stanisavljević, N., Vukotić, G., Pastor, F. T., Suznjević, D., Jovanović, Ž., Strahinić, I., Fira, Đ.,& Radović, S. S.. (2015). Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 67(3), 1033-1042.
https://doi.org/10.2298/ABS150130066S

Stanisavljević N, Vukotić G, Pastor FT, Suznjević D, Jovanović Ž, Strahinić I, Fira Đ, Radović SS. Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria. in Archives of Biological Sciences. 2015;67(3):1033-1042.
doi:10.2298/ABS150130066S .

Stanisavljević, Nemanja, Vukotić, Goran , Pastor, Ferenc T., Suznjević, Desanka, Jovanović, Živko, Strahinić, Ivana, Fira, Đorđe, Radović, Svetlana S., "Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria" in Archives of Biological Sciences, 67, no. 3 (2015):1033-1042,
https://doi.org/10.2298/ABS150130066S . .