TY  - JOUR
AU  - Vicentić, Jelena Marjanovic
AU  - Drakulić, Danijela
AU  - Garcia, Idoia
AU  - Vuković, Vladanka
AU  - Aldaz, Paula
AU  - Puskas, Nela
AU  - Nikolić, Igor
AU  - Tasić, Goran
AU  - Raicević, Savo
AU  - Garros-Regulez, Laura
AU  - Sampron, Nicolas
AU  - Atkinson, Michael J.
AU  - Anastasov, Nataša
AU  - Matheu, Ander
AU  - Stevanović, Milena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1285
AB  - PurposeGlioblastoma is the most common and lethal adult brain tumor. Despite current therapeutic strategies, including surgery, radiation and chemotherapy, the median survival of glioblastoma patients is 15months. The development of this tumor depends on a sub-population of glioblastoma stem cells governing tumor propagation and therapy resistance. SOX3 plays a role in both normal neural development and carcinogenesis. However, little is known about its role in glioblastoma. Thus, the aim of this work was to elucidate the role of SOX3 in glioblastoma.MethodsSOX3 expression was assessed using real-time quantitative PCR (RT-qPCR), Western blotting and immunohistochemistry. MTT, immunocytochemistry and Transwell assays were used to evaluate the effects of exogenous SOX3 overexpression on the viability, proliferation, migration and invasion of glioblastoma cells, respectively. The expression of Hedgehog signaling pathway components and autophagy markers was assessed using RT-qPCR and Western blot analyses, respectively.ResultsHigher levels of SOX3 expression were detected in a subset of primary glioblastoma samples compared to those in non-tumoral brain tissues. Exogenous overexpression of this gene was found to increase the proliferation, viability, migration and invasion of glioblastoma cells. We also found that SOX3 up-regulation was accompanied by an enhanced activity of the Hedgehog signaling pathway and by suppression of autophagy in glioblastoma cells. Additionally, we found that SOX3 expression was elevated in patient-derived glioblastoma stem cells, as well as in oncospheres derived from glioblastoma cell lines, compared to their differentiated counterparts, implying that SOX3 expression is associated with the undifferentiated state of glioblastoma cells.ConclusionFrom our data we conclude that SOX3 can promote the malignant behavior of glioblastoma cells.
PB  - Springer, Dordrecht
T2  - Cellular Oncology
T1  - SOX3 can promote the malignant behavior of glioblastoma cells
EP  - 54
IS  - 1
SP  - 41
VL  - 42
DO  - 10.1007/s13402-018-0405-5
ER  - 

@article{
author = "Vicentić, Jelena Marjanovic and Drakulić, Danijela and Garcia, Idoia and Vuković, Vladanka and Aldaz, Paula and Puskas, Nela and Nikolić, Igor and Tasić, Goran and Raicević, Savo and Garros-Regulez, Laura and Sampron, Nicolas and Atkinson, Michael J. and Anastasov, Nataša and Matheu, Ander and Stevanović, Milena",
year = "2019",
abstract = "PurposeGlioblastoma is the most common and lethal adult brain tumor. Despite current therapeutic strategies, including surgery, radiation and chemotherapy, the median survival of glioblastoma patients is 15months. The development of this tumor depends on a sub-population of glioblastoma stem cells governing tumor propagation and therapy resistance. SOX3 plays a role in both normal neural development and carcinogenesis. However, little is known about its role in glioblastoma. Thus, the aim of this work was to elucidate the role of SOX3 in glioblastoma.MethodsSOX3 expression was assessed using real-time quantitative PCR (RT-qPCR), Western blotting and immunohistochemistry. MTT, immunocytochemistry and Transwell assays were used to evaluate the effects of exogenous SOX3 overexpression on the viability, proliferation, migration and invasion of glioblastoma cells, respectively. The expression of Hedgehog signaling pathway components and autophagy markers was assessed using RT-qPCR and Western blot analyses, respectively.ResultsHigher levels of SOX3 expression were detected in a subset of primary glioblastoma samples compared to those in non-tumoral brain tissues. Exogenous overexpression of this gene was found to increase the proliferation, viability, migration and invasion of glioblastoma cells. We also found that SOX3 up-regulation was accompanied by an enhanced activity of the Hedgehog signaling pathway and by suppression of autophagy in glioblastoma cells. Additionally, we found that SOX3 expression was elevated in patient-derived glioblastoma stem cells, as well as in oncospheres derived from glioblastoma cell lines, compared to their differentiated counterparts, implying that SOX3 expression is associated with the undifferentiated state of glioblastoma cells.ConclusionFrom our data we conclude that SOX3 can promote the malignant behavior of glioblastoma cells.",
publisher = "Springer, Dordrecht",
journal = "Cellular Oncology",
title = "SOX3 can promote the malignant behavior of glioblastoma cells",
pages = "54-41",
number = "1",
volume = "42",
doi = "10.1007/s13402-018-0405-5"
}

Vicentić, J. M., Drakulić, D., Garcia, I., Vuković, V., Aldaz, P., Puskas, N., Nikolić, I., Tasić, G., Raicević, S., Garros-Regulez, L., Sampron, N., Atkinson, M. J., Anastasov, N., Matheu, A.,& Stevanović, M.. (2019). SOX3 can promote the malignant behavior of glioblastoma cells. in Cellular Oncology
Springer, Dordrecht., 42(1), 41-54.
https://doi.org/10.1007/s13402-018-0405-5

Vicentić JM, Drakulić D, Garcia I, Vuković V, Aldaz P, Puskas N, Nikolić I, Tasić G, Raicević S, Garros-Regulez L, Sampron N, Atkinson MJ, Anastasov N, Matheu A, Stevanović M. SOX3 can promote the malignant behavior of glioblastoma cells. in Cellular Oncology. 2019;42(1):41-54.
doi:10.1007/s13402-018-0405-5 .

Vicentić, Jelena Marjanovic, Drakulić, Danijela, Garcia, Idoia, Vuković, Vladanka, Aldaz, Paula, Puskas, Nela, Nikolić, Igor, Tasić, Goran, Raicević, Savo, Garros-Regulez, Laura, Sampron, Nicolas, Atkinson, Michael J., Anastasov, Nataša, Matheu, Ander, Stevanović, Milena, "SOX3 can promote the malignant behavior of glioblastoma cells" in Cellular Oncology, 42, no. 1 (2019):41-54,
https://doi.org/10.1007/s13402-018-0405-5 . .

TY  - JOUR
AU  - Zaletel, Ivan
AU  - Schwirtlich, Marija
AU  - Perović, Milka
AU  - Jovanović, Mirna
AU  - Stevanović, Milena
AU  - Kanazir, Selma
AU  - Puskas, Nela
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1101
AB  - Dysregulation of neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus has been related to cognitive deficits and memory loss in neurodegenerative diseases, such as Alzheimer's disease (AD). Members of the B group of SOX transcription factors play critical roles in regulating neurogenesis in the embryonic and adult nervous system, including maintaining the multipotency, renewal, and cell fate decision of neural stem/progenitor cells. The aim of the present study was to evaluate the expression patterns of selected SOXB proteins in the SGZ, of 8-week-old male and female 5xFAD mice, which represent a transgenic model of AD with a severe and very early development of amyloid pathology Immunohistochemical analysis showed a significant decrease in the number of cells expressing SOX1, SOX2, and SOX21 transcription factors within the SGZ of 5xFAD mice in comparison to their non-transgenic counterparts which coincidences with reduced number of doublecortin immunoreactive immature neurons found in Tg males. Despite observed changes in expressional pattern of examined SOXB proteins, the proliferative capacity evaluated by the number of Ki-67 immunoreactive cells remained unaffected in transgenic mice of both genders. Based on our results, we suggest that SOXB proteins might be considered as new biomarkers for the detection of early impairments in adult neurogenesis in different animal models or/and new targets in human regenerative medicine.
PB  - IOS Press, Amsterdam
T2  - Journal of Alzheimers Disease
T1  - Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors
EP  - 976
IS  - 3
SP  - 963
VL  - 65
DO  - 10.3233/JAD-180277
ER  - 

@article{
author = "Zaletel, Ivan and Schwirtlich, Marija and Perović, Milka and Jovanović, Mirna and Stevanović, Milena and Kanazir, Selma and Puskas, Nela",
year = "2018",
abstract = "Dysregulation of neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus has been related to cognitive deficits and memory loss in neurodegenerative diseases, such as Alzheimer's disease (AD). Members of the B group of SOX transcription factors play critical roles in regulating neurogenesis in the embryonic and adult nervous system, including maintaining the multipotency, renewal, and cell fate decision of neural stem/progenitor cells. The aim of the present study was to evaluate the expression patterns of selected SOXB proteins in the SGZ, of 8-week-old male and female 5xFAD mice, which represent a transgenic model of AD with a severe and very early development of amyloid pathology Immunohistochemical analysis showed a significant decrease in the number of cells expressing SOX1, SOX2, and SOX21 transcription factors within the SGZ of 5xFAD mice in comparison to their non-transgenic counterparts which coincidences with reduced number of doublecortin immunoreactive immature neurons found in Tg males. Despite observed changes in expressional pattern of examined SOXB proteins, the proliferative capacity evaluated by the number of Ki-67 immunoreactive cells remained unaffected in transgenic mice of both genders. Based on our results, we suggest that SOXB proteins might be considered as new biomarkers for the detection of early impairments in adult neurogenesis in different animal models or/and new targets in human regenerative medicine.",
publisher = "IOS Press, Amsterdam",
journal = "Journal of Alzheimers Disease",
title = "Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors",
pages = "976-963",
number = "3",
volume = "65",
doi = "10.3233/JAD-180277"
}

Zaletel, I., Schwirtlich, M., Perović, M., Jovanović, M., Stevanović, M., Kanazir, S.,& Puskas, N.. (2018). Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors. in Journal of Alzheimers Disease
IOS Press, Amsterdam., 65(3), 963-976.
https://doi.org/10.3233/JAD-180277

Zaletel I, Schwirtlich M, Perović M, Jovanović M, Stevanović M, Kanazir S, Puskas N. Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors. in Journal of Alzheimers Disease. 2018;65(3):963-976.
doi:10.3233/JAD-180277 .

Zaletel, Ivan, Schwirtlich, Marija, Perović, Milka, Jovanović, Mirna, Stevanović, Milena, Kanazir, Selma, Puskas, Nela, "Early Impairments of Hippocampal Neurogenesis in 5xFAD Mouse Model of Alzheimer's Disease Are Associated with Altered Expression of SOXB Transcription Factors" in Journal of Alzheimers Disease, 65, no. 3 (2018):963-976,
https://doi.org/10.3233/JAD-180277 . .

TY  - JOUR
AU  - Rakocević, Jelena
AU  - Kojić, Snežana
AU  - Orlić, Dejan
AU  - Stanković, Goran
AU  - Ostojić, Miodrag
AU  - Petrović, Olga
AU  - Zaletel, Ivan
AU  - Puskas, Nela
AU  - Todorović, Vera
AU  - Labudović-Borović, Milica
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/979
AB  - Introduction: Angiogenesis is the growth of both new vascular and lymphatic blood vessels from the existing vasculature. During this process, blood endothelial cells (BECs) and lymphatic endothelial cells (LECs) express specific markers, which help their discrimination and easier identification. Since the coronary thrombi material aspirated from patients with ST-elevation myocardial infarction (STEMI) proved as good angiogenesis model, we investigated the expression of CD34 and CD31 as BECs markers, and D2-40, LYVE-1 and VEGFR3 as LEC markers in this material. Materials and methods: Aspirated thrombi were stained immunohistochemically for CD34, CD31, D2-40, LYVE-1 and VEGFR3. Organizational patterns of immunopositive cells were graded as single cells, clusters or microvessels. Double immunofluorescence for CD31, D2-40, LYVE-1 and VEGRF3 was done. Thrombi were also graded as fresh ( lt 1 day old), lytic (1-5 days old) and organized ( gt 5 days old). Results: Serial sections of aspirated thrombi showed concordant BEC and LEC markers immunopositivity. Double immunoflorescence proved co-expression of CD31 and LEC markers on the same cells. Cells expressing LEC markers organized in clusters and microvessels were mainly present in lytic and organized thrombi. Conclusion: Co-expression of BEC and LEC markers on the same non-tumorous cell during thrombus neovascularization indicates existing in vivo plasticity of endothelial cells under non-tumorous pathological conditions. It also points that CD34 and CD31 on one hand, and D2-40, LYVE-1 and VEGFR3 immunostaining on the other hand, cannot solely be a reliable indicators whether vessel is lymphatic or not.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Experimental and Molecular Pathology
T1  - Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction
EP  - 38
IS  - 1
SP  - 31
VL  - 100
DO  - 10.1016/j.yexmp.2015.11.028
ER  - 

@article{
author = "Rakocević, Jelena and Kojić, Snežana and Orlić, Dejan and Stanković, Goran and Ostojić, Miodrag and Petrović, Olga and Zaletel, Ivan and Puskas, Nela and Todorović, Vera and Labudović-Borović, Milica",
year = "2016",
abstract = "Introduction: Angiogenesis is the growth of both new vascular and lymphatic blood vessels from the existing vasculature. During this process, blood endothelial cells (BECs) and lymphatic endothelial cells (LECs) express specific markers, which help their discrimination and easier identification. Since the coronary thrombi material aspirated from patients with ST-elevation myocardial infarction (STEMI) proved as good angiogenesis model, we investigated the expression of CD34 and CD31 as BECs markers, and D2-40, LYVE-1 and VEGFR3 as LEC markers in this material. Materials and methods: Aspirated thrombi were stained immunohistochemically for CD34, CD31, D2-40, LYVE-1 and VEGFR3. Organizational patterns of immunopositive cells were graded as single cells, clusters or microvessels. Double immunofluorescence for CD31, D2-40, LYVE-1 and VEGRF3 was done. Thrombi were also graded as fresh ( lt 1 day old), lytic (1-5 days old) and organized ( gt 5 days old). Results: Serial sections of aspirated thrombi showed concordant BEC and LEC markers immunopositivity. Double immunoflorescence proved co-expression of CD31 and LEC markers on the same cells. Cells expressing LEC markers organized in clusters and microvessels were mainly present in lytic and organized thrombi. Conclusion: Co-expression of BEC and LEC markers on the same non-tumorous cell during thrombus neovascularization indicates existing in vivo plasticity of endothelial cells under non-tumorous pathological conditions. It also points that CD34 and CD31 on one hand, and D2-40, LYVE-1 and VEGFR3 immunostaining on the other hand, cannot solely be a reliable indicators whether vessel is lymphatic or not.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Experimental and Molecular Pathology",
title = "Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction",
pages = "38-31",
number = "1",
volume = "100",
doi = "10.1016/j.yexmp.2015.11.028"
}

Rakocević, J., Kojić, S., Orlić, D., Stanković, G., Ostojić, M., Petrović, O., Zaletel, I., Puskas, N., Todorović, V.,& Labudović-Borović, M.. (2016). Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction. in Experimental and Molecular Pathology
Academic Press Inc Elsevier Science, San Diego., 100(1), 31-38.
https://doi.org/10.1016/j.yexmp.2015.11.028

Rakocević J, Kojić S, Orlić D, Stanković G, Ostojić M, Petrović O, Zaletel I, Puskas N, Todorović V, Labudović-Borović M. Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction. in Experimental and Molecular Pathology. 2016;100(1):31-38.
doi:10.1016/j.yexmp.2015.11.028 .

Rakocević, Jelena, Kojić, Snežana, Orlić, Dejan, Stanković, Goran, Ostojić, Miodrag, Petrović, Olga, Zaletel, Ivan, Puskas, Nela, Todorović, Vera, Labudović-Borović, Milica, "Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction" in Experimental and Molecular Pathology, 100, no. 1 (2016):31-38,
https://doi.org/10.1016/j.yexmp.2015.11.028 . .