TY  - JOUR
AU  - Popović, Nikola
AU  - Terzić-Vidojević, Amarela
AU  - Brdarić, Emilija
AU  - Bajić, Svetlana Soković
AU  - Đokić, Jelena
AU  - Živković, Milica
AU  - Veljović, Katarina
PY  - 2023
UR  - https://aseestant.ceon.rs/index.php/arhfarm/article/view/47047
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2296
AB  - One of the major genera of the lactic acid bacteria family, Enterococcus sp., has a controversial status, reflected in the fact that enterococci are utilized as starter cultures and probiotics, in addition to being known to cause nosocomial infections. The qualified presumption of the safety list and the widely acknowledged safe status for Enterococcus species are absent. Rich sources of Enterococcus faecium species with possible probiotic characteristics can be found in artisanal dairy products, typically made from raw milk. To further understand the probiotic potential and health-promoting effects, this study looked at the presence of virulence factors and adhesion properties of En. faecium isolated from artisanal dairy products from Western Balkan countries.
PB  - Centre for Evaluation in Education and Science
T2  - Archives of Pharmacy
T1  - Probiotic Potential of Dairy Western Balkan Countries Enterococcus faecium strains
T1  - Probiotički potencijal sojeva Enterococcus faecium izolovanih iz mlečnih proizvoda sa područja Zapadnog Balkana
EP  - 570
IS  - Notebook 6
SP  - 554
VL  - 73
DO  - 10.5937/arhfarm73-47047
ER  - 

@article{
author = "Popović, Nikola and Terzić-Vidojević, Amarela and Brdarić, Emilija and Bajić, Svetlana Soković and Đokić, Jelena and Živković, Milica and Veljović, Katarina",
year = "2023",
abstract = "One of the major genera of the lactic acid bacteria family, Enterococcus sp., has a controversial status, reflected in the fact that enterococci are utilized as starter cultures and probiotics, in addition to being known to cause nosocomial infections. The qualified presumption of the safety list and the widely acknowledged safe status for Enterococcus species are absent. Rich sources of Enterococcus faecium species with possible probiotic characteristics can be found in artisanal dairy products, typically made from raw milk. To further understand the probiotic potential and health-promoting effects, this study looked at the presence of virulence factors and adhesion properties of En. faecium isolated from artisanal dairy products from Western Balkan countries.",
publisher = "Centre for Evaluation in Education and Science",
journal = "Archives of Pharmacy",
title = "Probiotic Potential of Dairy Western Balkan Countries Enterococcus faecium strains, Probiotički potencijal sojeva Enterococcus faecium izolovanih iz mlečnih proizvoda sa područja Zapadnog Balkana",
pages = "570-554",
number = "Notebook 6",
volume = "73",
doi = "10.5937/arhfarm73-47047"
}

Popović, N., Terzić-Vidojević, A., Brdarić, E., Bajić, S. S., Đokić, J., Živković, M.,& Veljović, K.. (2023). Probiotic Potential of Dairy Western Balkan Countries Enterococcus faecium strains. in Archives of Pharmacy
Centre for Evaluation in Education and Science., 73(Notebook 6), 554-570.
https://doi.org/10.5937/arhfarm73-47047

Popović N, Terzić-Vidojević A, Brdarić E, Bajić SS, Đokić J, Živković M, Veljović K. Probiotic Potential of Dairy Western Balkan Countries Enterococcus faecium strains. in Archives of Pharmacy. 2023;73(Notebook 6):554-570.
doi:10.5937/arhfarm73-47047 .

Popović, Nikola, Terzić-Vidojević, Amarela, Brdarić, Emilija, Bajić, Svetlana Soković, Đokić, Jelena, Živković, Milica, Veljović, Katarina, "Probiotic Potential of Dairy Western Balkan Countries Enterococcus faecium strains" in Archives of Pharmacy, 73, no. Notebook 6 (2023):554-570,
https://doi.org/10.5937/arhfarm73-47047 . .

TY  - JOUR
AU  - Popović, Nikola
AU  - Stevanović, Dušan
AU  - Radojević, Dušan
AU  - Veljović, Katarina
AU  - Đokić, Jelena
AU  - Golić, Nataša
AU  - Terzić-Vidojević, Amarela
PY  - 2023
UR  - https://www.mdpi.com/2076-2607/11/12/2844
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2209
AB  - This study aimed to explore the probiogenomic characteristics of artisanal bacteriocin-producing Enterococcus faecium BGZLM1-5 and its potential application in reducing Listeria monocytogenes in a milk model. The BGZLM1-5 strain was isolated from raw cow’s milk from households in the Zlatar Mountain region. The whole genome sequencing approach and bioinformatics analyses reveal that the strain BGZLM1-5 is non-pathogenic to humans. Bacteriocin-containing supernatant was thermally stable and antimicrobial activity retained 75% of the initial activity compared with that of the control after treatment at 90 °C for 30 min. Antimicrobial activity maintained relative stability at pH 3–11 and retained 62.5% of the initial activity compared with that of the control after treatment at pH 1, 2, and 12. The highest activity of the partially purified bacteriocin was obtained after precipitation at 40% saturation with ammonium sulfate and further purification by mixing with chloroform. Applying 3% and 5% (v/v) of the bacteriocin-containing supernatant and 0.5% (v/v) of the partially purified bacteriocin decreased the viable number of L. monocytogenes ATCC19111 after three days of milk storage by 23.5%, 63.5%, and 58.9%, respectively.
T2  - Microorganisms
T1  - Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing Enterococcus faecium BGZLM1-5 in the Reduction in the Abundance of Listeria monocytogenes ATCC19111 in a Milk Model
IS  - 12
SP  - 2844
VL  - 11
DO  - 10.3390/microorganisms11122844
ER  - 

@article{
author = "Popović, Nikola and Stevanović, Dušan and Radojević, Dušan and Veljović, Katarina and Đokić, Jelena and Golić, Nataša and Terzić-Vidojević, Amarela",
year = "2023",
abstract = "This study aimed to explore the probiogenomic characteristics of artisanal bacteriocin-producing Enterococcus faecium BGZLM1-5 and its potential application in reducing Listeria monocytogenes in a milk model. The BGZLM1-5 strain was isolated from raw cow’s milk from households in the Zlatar Mountain region. The whole genome sequencing approach and bioinformatics analyses reveal that the strain BGZLM1-5 is non-pathogenic to humans. Bacteriocin-containing supernatant was thermally stable and antimicrobial activity retained 75% of the initial activity compared with that of the control after treatment at 90 °C for 30 min. Antimicrobial activity maintained relative stability at pH 3–11 and retained 62.5% of the initial activity compared with that of the control after treatment at pH 1, 2, and 12. The highest activity of the partially purified bacteriocin was obtained after precipitation at 40% saturation with ammonium sulfate and further purification by mixing with chloroform. Applying 3% and 5% (v/v) of the bacteriocin-containing supernatant and 0.5% (v/v) of the partially purified bacteriocin decreased the viable number of L. monocytogenes ATCC19111 after three days of milk storage by 23.5%, 63.5%, and 58.9%, respectively.",
journal = "Microorganisms",
title = "Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing Enterococcus faecium BGZLM1-5 in the Reduction in the Abundance of Listeria monocytogenes ATCC19111 in a Milk Model",
number = "12",
pages = "2844",
volume = "11",
doi = "10.3390/microorganisms11122844"
}

Popović, N., Stevanović, D., Radojević, D., Veljović, K., Đokić, J., Golić, N.,& Terzić-Vidojević, A.. (2023). Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing Enterococcus faecium BGZLM1-5 in the Reduction in the Abundance of Listeria monocytogenes ATCC19111 in a Milk Model. in Microorganisms, 11(12), 2844.
https://doi.org/10.3390/microorganisms11122844

Popović N, Stevanović D, Radojević D, Veljović K, Đokić J, Golić N, Terzić-Vidojević A. Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing Enterococcus faecium BGZLM1-5 in the Reduction in the Abundance of Listeria monocytogenes ATCC19111 in a Milk Model. in Microorganisms. 2023;11(12):2844.
doi:10.3390/microorganisms11122844 .

Popović, Nikola, Stevanović, Dušan, Radojević, Dušan, Veljović, Katarina, Đokić, Jelena, Golić, Nataša, Terzić-Vidojević, Amarela, "Insight into the Postbiotic Potential of the Autochthonous Bacteriocin-Producing Enterococcus faecium BGZLM1-5 in the Reduction in the Abundance of Listeria monocytogenes ATCC19111 in a Milk Model" in Microorganisms, 11, no. 12 (2023):2844,
https://doi.org/10.3390/microorganisms11122844 . .

TY  - JOUR
AU  - Veljović, Katarina
AU  - Tesevic, Vele
AU  - Mitrović, Hristina
AU  - Stanković, Marija
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S2210803323000295
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2092
AB  - IntroductionOregano essential oil (OEO) is one of the most widely used essential oils worldwide due to its huge therapeutic benefits. Nevertheless, data on the effects of the endemic species Origanum minutiflorum, also known as wild or Turkish oregano, is scarce. On the other hand, various chronic lung diseases, characterised by persistent inflammation, oxidative stress, and common bacterial infections, do not have effective pharmacological therapy. Hence, the aim of this study is to examine the effects of wild oregano essential oil (WOEO) on human bronchial epithelial cells and lung pathogens.MethodsWe provided a detailed chemical composition of WOEO using GC-MS and GC-FID analysis. Anti-inflammatory effects of WOEO were analysed using the qRT-PCR and ELISA methods, while antioxidative properties were examined by using the dichlorofluorescein assay in BEAS-2B cells. Antibacterial activity was tested on lung pathogens by using the agar diffusion assay.ResultsThe major constituents of WOEO, analysed in this study, were carvacrol, linalool, p-cymene, γ-terpinene, and (E)-caryophyllene. We found that treatment with WOEO attenuated LPS-induced IL8 gene expression and hydrogen peroxide-induced oxidative stress in BEAS-2B cells. Moreover, WOEO showed an inhibitory effect on pathogenic bacteria Acinetobacter baumannii and highly resistant Klebsiella pneumoniae commonly seen in healthcare-associated pneumonia.ConclusionOur work presents new insights into the anti-inflammatory, antioxidative, and antimicrobial properties of WOEO which may be used as a simple and local treatment in various chronic lung diseases.
T2  - Journal of Herbal Medicine
T1  - Essential oil of Origanum minutiflorum exhibits anti-inflammatory and antioxidative effects in human bronchial cells and antimicrobial activity on lung pathogens
SP  - 100651
VL  - 39
DO  - 10.1016/j.hermed.2023.100651
ER  - 

@article{
author = "Veljović, Katarina and Tesevic, Vele and Mitrović, Hristina and Stanković, Marija",
year = "2023",
abstract = "IntroductionOregano essential oil (OEO) is one of the most widely used essential oils worldwide due to its huge therapeutic benefits. Nevertheless, data on the effects of the endemic species Origanum minutiflorum, also known as wild or Turkish oregano, is scarce. On the other hand, various chronic lung diseases, characterised by persistent inflammation, oxidative stress, and common bacterial infections, do not have effective pharmacological therapy. Hence, the aim of this study is to examine the effects of wild oregano essential oil (WOEO) on human bronchial epithelial cells and lung pathogens.MethodsWe provided a detailed chemical composition of WOEO using GC-MS and GC-FID analysis. Anti-inflammatory effects of WOEO were analysed using the qRT-PCR and ELISA methods, while antioxidative properties were examined by using the dichlorofluorescein assay in BEAS-2B cells. Antibacterial activity was tested on lung pathogens by using the agar diffusion assay.ResultsThe major constituents of WOEO, analysed in this study, were carvacrol, linalool, p-cymene, γ-terpinene, and (E)-caryophyllene. We found that treatment with WOEO attenuated LPS-induced IL8 gene expression and hydrogen peroxide-induced oxidative stress in BEAS-2B cells. Moreover, WOEO showed an inhibitory effect on pathogenic bacteria Acinetobacter baumannii and highly resistant Klebsiella pneumoniae commonly seen in healthcare-associated pneumonia.ConclusionOur work presents new insights into the anti-inflammatory, antioxidative, and antimicrobial properties of WOEO which may be used as a simple and local treatment in various chronic lung diseases.",
journal = "Journal of Herbal Medicine",
title = "Essential oil of Origanum minutiflorum exhibits anti-inflammatory and antioxidative effects in human bronchial cells and antimicrobial activity on lung pathogens",
pages = "100651",
volume = "39",
doi = "10.1016/j.hermed.2023.100651"
}

Veljović, K., Tesevic, V., Mitrović, H.,& Stanković, M.. (2023). Essential oil of Origanum minutiflorum exhibits anti-inflammatory and antioxidative effects in human bronchial cells and antimicrobial activity on lung pathogens. in Journal of Herbal Medicine, 39, 100651.
https://doi.org/10.1016/j.hermed.2023.100651

Veljović K, Tesevic V, Mitrović H, Stanković M. Essential oil of Origanum minutiflorum exhibits anti-inflammatory and antioxidative effects in human bronchial cells and antimicrobial activity on lung pathogens. in Journal of Herbal Medicine. 2023;39:100651.
doi:10.1016/j.hermed.2023.100651 .

Veljović, Katarina, Tesevic, Vele, Mitrović, Hristina, Stanković, Marija, "Essential oil of Origanum minutiflorum exhibits anti-inflammatory and antioxidative effects in human bronchial cells and antimicrobial activity on lung pathogens" in Journal of Herbal Medicine, 39 (2023):100651,
https://doi.org/10.1016/j.hermed.2023.100651 . .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Jakovljević, Stefan
AU  - Popović, Nikola
AU  - Radojević, Dušan
AU  - Veljović, Katarina
AU  - Golić, Nataša
AU  - Terzić-Vidojević, Amarela
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2091
AB  - Yogurt represent one of the oldest fermented foods containing viable lactic acid bacteria and many bioactive compounds that could exhibit beneficial effects on human health and train our immune system to better respond to invading pathogens. Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus are commonly used for yogurt preparation under controlled temperature and environmental conditions. In this study, we investigated probiotic features of S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 strains isolated from artisanal sour milk and yogurt by using Caenorhabditis elegans as an in vivo model system. Further, we evaluated content of total fat, saturated fatty acids, proteins, and lactose, as well as vitamins and AA of yogurt prepared from above-mentioned starter cultures during 21 d of storage at 4°C to get insights of final product stability. We showed that S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 strains applied in combination upregulated the expression of autophagy-related genes in C. elegans. Beside autophagy, we observed activation of TIR-1-dependent transcription of lysozyme-like antimicrobial genes involved in the immune defense of C. elegans. Upregulation of these genes strongly correlates with an increase in the longevity of the worms fed with yogurt culture bacteria. Further, we showed that yogurt prepared with S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21, as a final product, is rich with vitamin B2 and dominant AA known by their prolongevity properties. Taken together, our study pointed to the beneficial features of the tested starter cultures and yogurt and highlighted their potential to be used as a fermented food with added-value properties.
PB  - Elsevier
PB  - American Dairy Science Association
T2  - Journal of Dairy Science
T2  - Journal of Dairy ScienceJournal of Dairy Science
T1  - Stability and bioactive compounds assessment of yogurt containing novel natural starter cultures with the ability to promote longevity in Caenorhabditis elegans
EP  - 7460
IS  - 11
SP  - 7447
VL  - 106
DO  - 10.3168/jds.2023-23342
ER  - 

@article{
author = "Dinić, Miroslav and Jakovljević, Stefan and Popović, Nikola and Radojević, Dušan and Veljović, Katarina and Golić, Nataša and Terzić-Vidojević, Amarela",
year = "2023",
abstract = "Yogurt represent one of the oldest fermented foods containing viable lactic acid bacteria and many bioactive compounds that could exhibit beneficial effects on human health and train our immune system to better respond to invading pathogens. Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus are commonly used for yogurt preparation under controlled temperature and environmental conditions. In this study, we investigated probiotic features of S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 strains isolated from artisanal sour milk and yogurt by using Caenorhabditis elegans as an in vivo model system. Further, we evaluated content of total fat, saturated fatty acids, proteins, and lactose, as well as vitamins and AA of yogurt prepared from above-mentioned starter cultures during 21 d of storage at 4°C to get insights of final product stability. We showed that S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 strains applied in combination upregulated the expression of autophagy-related genes in C. elegans. Beside autophagy, we observed activation of TIR-1-dependent transcription of lysozyme-like antimicrobial genes involved in the immune defense of C. elegans. Upregulation of these genes strongly correlates with an increase in the longevity of the worms fed with yogurt culture bacteria. Further, we showed that yogurt prepared with S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21, as a final product, is rich with vitamin B2 and dominant AA known by their prolongevity properties. Taken together, our study pointed to the beneficial features of the tested starter cultures and yogurt and highlighted their potential to be used as a fermented food with added-value properties.",
publisher = "Elsevier, American Dairy Science Association",
journal = "Journal of Dairy Science, Journal of Dairy ScienceJournal of Dairy Science",
title = "Stability and bioactive compounds assessment of yogurt containing novel natural starter cultures with the ability to promote longevity in Caenorhabditis elegans",
pages = "7460-7447",
number = "11",
volume = "106",
doi = "10.3168/jds.2023-23342"
}

Dinić, M., Jakovljević, S., Popović, N., Radojević, D., Veljović, K., Golić, N.,& Terzić-Vidojević, A.. (2023). Stability and bioactive compounds assessment of yogurt containing novel natural starter cultures with the ability to promote longevity in Caenorhabditis elegans. in Journal of Dairy Science
Elsevier., 106(11), 7447-7460.
https://doi.org/10.3168/jds.2023-23342

Dinić M, Jakovljević S, Popović N, Radojević D, Veljović K, Golić N, Terzić-Vidojević A. Stability and bioactive compounds assessment of yogurt containing novel natural starter cultures with the ability to promote longevity in Caenorhabditis elegans. in Journal of Dairy Science. 2023;106(11):7447-7460.
doi:10.3168/jds.2023-23342 .

Dinić, Miroslav, Jakovljević, Stefan, Popović, Nikola, Radojević, Dušan, Veljović, Katarina, Golić, Nataša, Terzić-Vidojević, Amarela, "Stability and bioactive compounds assessment of yogurt containing novel natural starter cultures with the ability to promote longevity in Caenorhabditis elegans" in Journal of Dairy Science, 106, no. 11 (2023):7447-7460,
https://doi.org/10.3168/jds.2023-23342 . .

TY  - JOUR
AU  - Mitrović, Miloš
AU  - Stanković-Popović, Verica
AU  - Tolinački, Maja
AU  - Golić, Nataša
AU  - Soković Bajić, Svetlana
AU  - Veljović, Katarina
AU  - Nastasijević, Branislav
AU  - Soldatović, Ivan
AU  - Svorcan, Petar
AU  - Dimković, Nada
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S1051227622001522
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1753
AB  - ObjectiveAltering dysbiotic gut flora through synbiotic supplementation has recently been recognized as a potential treatment strategy to reduce the levels of gut-derived uremic toxins and decrease inflammation. Assessing its efficacy and safety has been the main goal of our randomized, double-blind, placebo-controlled study.MethodsA total of 34 nondialyzed chronic kidney disease patients, aged ≥18 years, with an estimated glomerular filtration rate between 15 and 45 mL/minute, were randomized either to an intervention group (n = 17), receiving synbiotic (Lactobacillus acidophilus, Lactobacillus casei, and Bifidobacterium lactis, 32 billion colony forming units per day plus 3.2 g of inulin), or control group (n = 17), receiving placebo during 12 weeks. The impact of treatment on the dynamic of serum levels of gut-derived uremic toxins, total serum indoxyl sulfate, p-cresyl sulfate, and trimethylamine N-oxide, was defined as the primary outcome of the study. Secondary outcomes included changes in the stool microbiome, serum interleukin-6 levels, high-sensitivity C-reactive protein, estimated glomerular filtration rate, albuminuria, diet, gastrointestinal symptom dynamics, and safety. Serum levels of uremic toxins were determined using ultraperformance liquid chromatography. The stool microbiome analysis was performed using the 16S ribosomal ribonucleic acid gene sequencing approach.ResultsSynbiotic treatment significantly modified gut microbiome with Bifidobacteria, Lactobacillus, and Subdoligranulum genera enrichment and consequently reduced serum level of indoxyl sulfate (ΔIS –21.5% vs. 5.3%, P < .001), improved estimated glomerular filtration rate (ΔeGFR 12% vs. 8%, P = .029), and decreased level of high-sensitivity C-reactive protein (–39.5 vs. –8.5%, P < .001) in treated patients. Two patients of the intervention arm complained of increased flatulence. No other safety issues were noted.ConclusionSynbiotics could be available, safe, and an effective therapeutic strategy we could use in daily practice in order to decrease levels of uremic toxins and microinflammation in chronic kidney disease patients.
T2  - Journal of Renal Nutrition
T2  - Journal of Renal NutritionJournal of Renal Nutrition
T1  - The impact of synbiotic treatment on the levels of gut-derived uremic zoxins, inflammation, and gut microbiome of chronic kidney disease patients - a randomized trial
EP  - 288
IS  - 2
SP  - 278
VL  - 33
DO  - 10.1053/j.jrn.2022.07.008
ER  - 

@article{
author = "Mitrović, Miloš and Stanković-Popović, Verica and Tolinački, Maja and Golić, Nataša and Soković Bajić, Svetlana and Veljović, Katarina and Nastasijević, Branislav and Soldatović, Ivan and Svorcan, Petar and Dimković, Nada",
year = "2023",
abstract = "ObjectiveAltering dysbiotic gut flora through synbiotic supplementation has recently been recognized as a potential treatment strategy to reduce the levels of gut-derived uremic toxins and decrease inflammation. Assessing its efficacy and safety has been the main goal of our randomized, double-blind, placebo-controlled study.MethodsA total of 34 nondialyzed chronic kidney disease patients, aged ≥18 years, with an estimated glomerular filtration rate between 15 and 45 mL/minute, were randomized either to an intervention group (n = 17), receiving synbiotic (Lactobacillus acidophilus, Lactobacillus casei, and Bifidobacterium lactis, 32 billion colony forming units per day plus 3.2 g of inulin), or control group (n = 17), receiving placebo during 12 weeks. The impact of treatment on the dynamic of serum levels of gut-derived uremic toxins, total serum indoxyl sulfate, p-cresyl sulfate, and trimethylamine N-oxide, was defined as the primary outcome of the study. Secondary outcomes included changes in the stool microbiome, serum interleukin-6 levels, high-sensitivity C-reactive protein, estimated glomerular filtration rate, albuminuria, diet, gastrointestinal symptom dynamics, and safety. Serum levels of uremic toxins were determined using ultraperformance liquid chromatography. The stool microbiome analysis was performed using the 16S ribosomal ribonucleic acid gene sequencing approach.ResultsSynbiotic treatment significantly modified gut microbiome with Bifidobacteria, Lactobacillus, and Subdoligranulum genera enrichment and consequently reduced serum level of indoxyl sulfate (ΔIS –21.5% vs. 5.3%, P < .001), improved estimated glomerular filtration rate (ΔeGFR 12% vs. 8%, P = .029), and decreased level of high-sensitivity C-reactive protein (–39.5 vs. –8.5%, P < .001) in treated patients. Two patients of the intervention arm complained of increased flatulence. No other safety issues were noted.ConclusionSynbiotics could be available, safe, and an effective therapeutic strategy we could use in daily practice in order to decrease levels of uremic toxins and microinflammation in chronic kidney disease patients.",
journal = "Journal of Renal Nutrition, Journal of Renal NutritionJournal of Renal Nutrition",
title = "The impact of synbiotic treatment on the levels of gut-derived uremic zoxins, inflammation, and gut microbiome of chronic kidney disease patients - a randomized trial",
pages = "288-278",
number = "2",
volume = "33",
doi = "10.1053/j.jrn.2022.07.008"
}

Mitrović, M., Stanković-Popović, V., Tolinački, M., Golić, N., Soković Bajić, S., Veljović, K., Nastasijević, B., Soldatović, I., Svorcan, P.,& Dimković, N.. (2023). The impact of synbiotic treatment on the levels of gut-derived uremic zoxins, inflammation, and gut microbiome of chronic kidney disease patients - a randomized trial. in Journal of Renal Nutrition, 33(2), 278-288.
https://doi.org/10.1053/j.jrn.2022.07.008

Mitrović M, Stanković-Popović V, Tolinački M, Golić N, Soković Bajić S, Veljović K, Nastasijević B, Soldatović I, Svorcan P, Dimković N. The impact of synbiotic treatment on the levels of gut-derived uremic zoxins, inflammation, and gut microbiome of chronic kidney disease patients - a randomized trial. in Journal of Renal Nutrition. 2023;33(2):278-288.
doi:10.1053/j.jrn.2022.07.008 .

Mitrović, Miloš, Stanković-Popović, Verica, Tolinački, Maja, Golić, Nataša, Soković Bajić, Svetlana, Veljović, Katarina, Nastasijević, Branislav, Soldatović, Ivan, Svorcan, Petar, Dimković, Nada, "The impact of synbiotic treatment on the levels of gut-derived uremic zoxins, inflammation, and gut microbiome of chronic kidney disease patients - a randomized trial" in Journal of Renal Nutrition, 33, no. 2 (2023):278-288,
https://doi.org/10.1053/j.jrn.2022.07.008 . .

TY  - JOUR
AU  - Babić, Mirjana
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Golić, Nataša
AU  - Radojković, Dragica
AU  - Stanković, Marija
PY  - 2023
UR  - https://doi.org/10.1093/jambio/lxad257
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2204
AB  - Chronic lung diseases are a major and increasing global health problem, commonly caused by cigarette smoke. We aimed to explore the antioxidant effects of lactic acid bacteria (LAB) against cigarette smoke in bronchial epithelial cells.The antioxidant effects of 21 heat-killed (HK) LAB strains were tested in cigarette smoke stimulated BEAS-2B cells and 3-D bronchospheres organoids. We showed that HK Lactiplantibacillus plantarum BGPKM22 possesses antioxidant activity against cigarette smoke, resistance to hydrogen peroxide, and free radical neutralizing activity. We demonstrated that HK BGPKM22 inhibited cigarette smoke induced expression of the Aryl hydrocarbon receptor (AhR) and Nuclear factor erythroid 2 related factor 2 (Nrf2) genes. The cell-free supernatant (SN) of BGPKM22 fully confirmed the effects of HK BGPKM22.For the first time, we revealed that HK and SN of L. plantarum BGPKM22 possess antioxidant activity and modulate AhR and Nrf2 gene expression in bronchial epithelial cells exposed to cigarette smoke.
T2  - Journal of Applied Microbiology
T1  - Antioxidant effect of lactic acid bacteria in human bronchial epithelial cells exposed to cigarette smoke
SP  - lxad257
VL  - n/a
DO  - 10.1093/jambio/lxad257
ER  - 

@article{
author = "Babić, Mirjana and Veljović, Katarina and Popović, Nikola and Golić, Nataša and Radojković, Dragica and Stanković, Marija",
year = "2023",
abstract = "Chronic lung diseases are a major and increasing global health problem, commonly caused by cigarette smoke. We aimed to explore the antioxidant effects of lactic acid bacteria (LAB) against cigarette smoke in bronchial epithelial cells.The antioxidant effects of 21 heat-killed (HK) LAB strains were tested in cigarette smoke stimulated BEAS-2B cells and 3-D bronchospheres organoids. We showed that HK Lactiplantibacillus plantarum BGPKM22 possesses antioxidant activity against cigarette smoke, resistance to hydrogen peroxide, and free radical neutralizing activity. We demonstrated that HK BGPKM22 inhibited cigarette smoke induced expression of the Aryl hydrocarbon receptor (AhR) and Nuclear factor erythroid 2 related factor 2 (Nrf2) genes. The cell-free supernatant (SN) of BGPKM22 fully confirmed the effects of HK BGPKM22.For the first time, we revealed that HK and SN of L. plantarum BGPKM22 possess antioxidant activity and modulate AhR and Nrf2 gene expression in bronchial epithelial cells exposed to cigarette smoke.",
journal = "Journal of Applied Microbiology",
title = "Antioxidant effect of lactic acid bacteria in human bronchial epithelial cells exposed to cigarette smoke",
pages = "lxad257",
volume = "n/a",
doi = "10.1093/jambio/lxad257"
}

Babić, M., Veljović, K., Popović, N., Golić, N., Radojković, D.,& Stanković, M.. (2023). Antioxidant effect of lactic acid bacteria in human bronchial epithelial cells exposed to cigarette smoke. in Journal of Applied Microbiology, n/a, lxad257.
https://doi.org/10.1093/jambio/lxad257

Babić M, Veljović K, Popović N, Golić N, Radojković D, Stanković M. Antioxidant effect of lactic acid bacteria in human bronchial epithelial cells exposed to cigarette smoke. in Journal of Applied Microbiology. 2023;n/a:lxad257.
doi:10.1093/jambio/lxad257 .

Babić, Mirjana, Veljović, Katarina, Popović, Nikola, Golić, Nataša, Radojković, Dragica, Stanković, Marija, "Antioxidant effect of lactic acid bacteria in human bronchial epithelial cells exposed to cigarette smoke" in Journal of Applied Microbiology, n/a (2023):lxad257,
https://doi.org/10.1093/jambio/lxad257 . .

TY  - JOUR
AU  - Stanković, Marija
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Kojić, Snežana
AU  - Dunjić Manevski, Sofija
AU  - Radojković, Dragica
AU  - Golić, Nataša
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1538
AB  - Bronchial epithelial cells are exposed to environmental influences, microbiota, and pathogens and also serve as a powerful effector that initiate and propagate inflammation by the release of proinflammatory mediators. Recent studies suggested that lung microbiota differ between inflammatory lung diseases and healthy lungs implicating their contribution in the modulation of lung immunity. Lactic acid bacteria (LAB) are natural inhabitants of healthy human lungs and also possess immunomodulatory effects, but so far, there are no studies investigating their anti-inflammatory potential in respiratory cells. In this study, we investigated immunomodulatory features of 21 natural LAB strains in lipopolysaccharide (LPS)-stimulated human bronchial epithelial cells (BEAS-2B). Our results show that several LAB strains reduced the expression of pro-inflammatory cytokine and chemokine genes. We also demonstrated that two LAB strains, Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22, effectively attenuated LPS-induced nuclear factor-kappa B (NF-kappa B) nuclear translocation. Moreover, BGZLS10-17 and BGPKM22 reduced the activation of p38, extracellular signal-related kinase (ERK), and c-Jun amino-terminal kinase (JNK) signaling cascade resulting in a reduction of pro-inflammatory mediator expressions in BEAS-2B cells. Collectively, the LAB strains BGZLS10-17 and BGPKM22 exhibited anti-inflammatory effects in BEAS-2B cells and could be employed to balance immune response in lungs and replenish diminished lung microbiota in chronic lung diseases.
PB  - MDPI, Basel
T2  - International Journal of Molecular Sciences
T1  - Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22 Exhibit Anti-Inflammatory Effect by Attenuation of NF-kappa B and MAPK Signaling in Human Bronchial Epithelial Cells
IS  - 10
VL  - 23
DO  - 10.3390/ijms23105547
ER  - 

@article{
author = "Stanković, Marija and Veljović, Katarina and Popović, Nikola and Kojić, Snežana and Dunjić Manevski, Sofija and Radojković, Dragica and Golić, Nataša",
year = "2022",
abstract = "Bronchial epithelial cells are exposed to environmental influences, microbiota, and pathogens and also serve as a powerful effector that initiate and propagate inflammation by the release of proinflammatory mediators. Recent studies suggested that lung microbiota differ between inflammatory lung diseases and healthy lungs implicating their contribution in the modulation of lung immunity. Lactic acid bacteria (LAB) are natural inhabitants of healthy human lungs and also possess immunomodulatory effects, but so far, there are no studies investigating their anti-inflammatory potential in respiratory cells. In this study, we investigated immunomodulatory features of 21 natural LAB strains in lipopolysaccharide (LPS)-stimulated human bronchial epithelial cells (BEAS-2B). Our results show that several LAB strains reduced the expression of pro-inflammatory cytokine and chemokine genes. We also demonstrated that two LAB strains, Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22, effectively attenuated LPS-induced nuclear factor-kappa B (NF-kappa B) nuclear translocation. Moreover, BGZLS10-17 and BGPKM22 reduced the activation of p38, extracellular signal-related kinase (ERK), and c-Jun amino-terminal kinase (JNK) signaling cascade resulting in a reduction of pro-inflammatory mediator expressions in BEAS-2B cells. Collectively, the LAB strains BGZLS10-17 and BGPKM22 exhibited anti-inflammatory effects in BEAS-2B cells and could be employed to balance immune response in lungs and replenish diminished lung microbiota in chronic lung diseases.",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22 Exhibit Anti-Inflammatory Effect by Attenuation of NF-kappa B and MAPK Signaling in Human Bronchial Epithelial Cells",
number = "10",
volume = "23",
doi = "10.3390/ijms23105547"
}

Stanković, M., Veljović, K., Popović, N., Kojić, S., Dunjić Manevski, S., Radojković, D.,& Golić, N.. (2022). Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22 Exhibit Anti-Inflammatory Effect by Attenuation of NF-kappa B and MAPK Signaling in Human Bronchial Epithelial Cells. in International Journal of Molecular Sciences
MDPI, Basel., 23(10).
https://doi.org/10.3390/ijms23105547

Stanković M, Veljović K, Popović N, Kojić S, Dunjić Manevski S, Radojković D, Golić N. Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22 Exhibit Anti-Inflammatory Effect by Attenuation of NF-kappa B and MAPK Signaling in Human Bronchial Epithelial Cells. in International Journal of Molecular Sciences. 2022;23(10).
doi:10.3390/ijms23105547 .

Stanković, Marija, Veljović, Katarina, Popović, Nikola, Kojić, Snežana, Dunjić Manevski, Sofija, Radojković, Dragica, Golić, Nataša, "Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22 Exhibit Anti-Inflammatory Effect by Attenuation of NF-kappa B and MAPK Signaling in Human Bronchial Epithelial Cells" in International Journal of Molecular Sciences, 23, no. 10 (2022),
https://doi.org/10.3390/ijms23105547 . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Tolinački, Maja
AU  - Golić, Nataša
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1464
AB  - The present study is focused on the safety, technological characteristics, and probiotic evaluation of Enterococcus species from different artisanal raw milk dairy products, mainly cheeses with ripening. Apart from proteolytic and lipolytic activities, most enterococci show the ability to metabolize citrate and convert it to various aromatic compounds. Long-ripened cheeses therefore have a specific flavor that makes them different from cheeses produced from thermally treated milk with commercial starter cultures. In addition, enterococci are producers of bacteriocins effective against spoilage and pathogenic bacteria, so they can be used as food preservatives. However, the use of enterococci in the dairy industry should be approached with caution. Although originating from food, enterococci strains may carry various virulence factors and antibiotic-resistance genes and can have many adverse effects on human health. Still, despite their controversial status, the use of enterococci in the food industry is not strictly regulated since the existence of these so-called desirable and undesirable traits in enterococci is a strain-dependent characteristic. To be specific, the results of many studies showed that there are some enterococci strains that are safe for use as starter cultures or as probiotics since they do not carry virulence factors and antibiotic-resistance genes. These strains even exhibit strong health-promoting effects such as stimulation of the immune response, anti-inflammatory activity, hypocholesterolemic action, and usefulness in prevention/treatment of some diseases.
PB  - MDPI, Basel
T2  - Foods
T1  - Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns
IS  - 11
VL  - 10
DO  - 10.3390/foods10112753
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Popović, Nikola and Tolinački, Maja and Golić, Nataša",
year = "2021",
abstract = "The present study is focused on the safety, technological characteristics, and probiotic evaluation of Enterococcus species from different artisanal raw milk dairy products, mainly cheeses with ripening. Apart from proteolytic and lipolytic activities, most enterococci show the ability to metabolize citrate and convert it to various aromatic compounds. Long-ripened cheeses therefore have a specific flavor that makes them different from cheeses produced from thermally treated milk with commercial starter cultures. In addition, enterococci are producers of bacteriocins effective against spoilage and pathogenic bacteria, so they can be used as food preservatives. However, the use of enterococci in the dairy industry should be approached with caution. Although originating from food, enterococci strains may carry various virulence factors and antibiotic-resistance genes and can have many adverse effects on human health. Still, despite their controversial status, the use of enterococci in the food industry is not strictly regulated since the existence of these so-called desirable and undesirable traits in enterococci is a strain-dependent characteristic. To be specific, the results of many studies showed that there are some enterococci strains that are safe for use as starter cultures or as probiotics since they do not carry virulence factors and antibiotic-resistance genes. These strains even exhibit strong health-promoting effects such as stimulation of the immune response, anti-inflammatory activity, hypocholesterolemic action, and usefulness in prevention/treatment of some diseases.",
publisher = "MDPI, Basel",
journal = "Foods",
title = "Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns",
number = "11",
volume = "10",
doi = "10.3390/foods10112753"
}

Terzić-Vidojević, A., Veljović, K., Popović, N., Tolinački, M.,& Golić, N.. (2021). Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns. in Foods
MDPI, Basel., 10(11).
https://doi.org/10.3390/foods10112753

Terzić-Vidojević A, Veljović K, Popović N, Tolinački M, Golić N. Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns. in Foods. 2021;10(11).
doi:10.3390/foods10112753 .

Terzić-Vidojević, Amarela, Veljović, Katarina, Popović, Nikola, Tolinački, Maja, Golić, Nataša, "Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns" in Foods, 10, no. 11 (2021),
https://doi.org/10.3390/foods10112753 . .

TY  - CONF
AU  - Blagojević, Veljko
AU  - Vujić, Vesna
AU  - Curuvija, Ivana
AU  - Veljović, Katarina
AU  - Soković Bajić, Svetlana
AU  - Stanojević, Stanislava
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1435
PB  - Wiley, Hoboken
C3  - European Journal of Immunology
T1  - Sex differences in the effects of early-life probiotic treatment on TNBS-induced colitis in rats
EP  - 311
SP  - 311
VL  - 51
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1435
ER  - 

@conference{
author = "Blagojević, Veljko and Vujić, Vesna and Curuvija, Ivana and Veljović, Katarina and Soković Bajić, Svetlana and Stanojević, Stanislava",
year = "2021",
publisher = "Wiley, Hoboken",
journal = "European Journal of Immunology",
title = "Sex differences in the effects of early-life probiotic treatment on TNBS-induced colitis in rats",
pages = "311-311",
volume = "51",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1435"
}

Blagojević, V., Vujić, V., Curuvija, I., Veljović, K., Soković Bajić, S.,& Stanojević, S.. (2021). Sex differences in the effects of early-life probiotic treatment on TNBS-induced colitis in rats. in European Journal of Immunology
Wiley, Hoboken., 51, 311-311.
https://hdl.handle.net/21.15107/rcub_imagine_1435

Blagojević V, Vujić V, Curuvija I, Veljović K, Soković Bajić S, Stanojević S. Sex differences in the effects of early-life probiotic treatment on TNBS-induced colitis in rats. in European Journal of Immunology. 2021;51:311-311.
https://hdl.handle.net/21.15107/rcub_imagine_1435 .

Blagojević, Veljko, Vujić, Vesna, Curuvija, Ivana, Veljović, Katarina, Soković Bajić, Svetlana, Stanojević, Stanislava, "Sex differences in the effects of early-life probiotic treatment on TNBS-induced colitis in rats" in European Journal of Immunology, 51 (2021):311-311,
https://hdl.handle.net/21.15107/rcub_imagine_1435 .

TY  - JOUR
AU  - Popović, Nikola
AU  - Brdarić, Emilija
AU  - Đokić, Jelena
AU  - Dinić, Miroslav
AU  - Veljović, Katarina
AU  - Golić, Nataša
AU  - Terzić-Vidojević, Amarela
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1344
AB  - Yogurt is a traditional fermented dairy product, prepared with starter cultures containing Streptococcus thermophilus and Lactobacillus bulgaricus that has gained widespread consumer acceptance as a healthy food. It is widely accepted that yogurt cultures have been recognized as probiotics, due to their beneficial effects on human health. In this study, we have characterized technological and health-promoting properties of autochthonous strains S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 isolated from artisanal sour milk and yogurt, respectively, in order to be used as functional yogurt starter cultures. Both BGKMJ1-36 and BGVLJ1-21 strains have the ability to form curd after five hours at 42 degrees C, hydrolyze alpha(s1)-, beta-, and kappa- casein, and to show antimicrobial activity toward Listeria monocytogenes. The strain BGKMJ1-36 produces exopolysaccharides important for rheological properties of the yogurt. The colonies of BGKMJ1-36 and BGVLJ1-21 strains that successfully survived transit of the yogurt through simulated gastrointestinal tract conditions have been tested for adhesion to intestinal epithelial Caco-2 cells. The results reveal that both strains adhere to Caco-2 cells and significantly upregulate the expression of autophagy-, tight junction proteins-, and anti-microbial peptides-related genes. Hence, both strains may be interesting for use as a novel functional starter culture for production of added-value yogurt with health-promoting properties.
PB  - MDPI, Basel
T2  - Microorganisms
T1  - Yogurt Produced by Novel Natural Starter Cultures Improves Gut Epithelial Barrier In Vitro
IS  - 10
VL  - 8
DO  - 10.3390/microorganisms8101586
ER  - 

@article{
author = "Popović, Nikola and Brdarić, Emilija and Đokić, Jelena and Dinić, Miroslav and Veljović, Katarina and Golić, Nataša and Terzić-Vidojević, Amarela",
year = "2020",
abstract = "Yogurt is a traditional fermented dairy product, prepared with starter cultures containing Streptococcus thermophilus and Lactobacillus bulgaricus that has gained widespread consumer acceptance as a healthy food. It is widely accepted that yogurt cultures have been recognized as probiotics, due to their beneficial effects on human health. In this study, we have characterized technological and health-promoting properties of autochthonous strains S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 isolated from artisanal sour milk and yogurt, respectively, in order to be used as functional yogurt starter cultures. Both BGKMJ1-36 and BGVLJ1-21 strains have the ability to form curd after five hours at 42 degrees C, hydrolyze alpha(s1)-, beta-, and kappa- casein, and to show antimicrobial activity toward Listeria monocytogenes. The strain BGKMJ1-36 produces exopolysaccharides important for rheological properties of the yogurt. The colonies of BGKMJ1-36 and BGVLJ1-21 strains that successfully survived transit of the yogurt through simulated gastrointestinal tract conditions have been tested for adhesion to intestinal epithelial Caco-2 cells. The results reveal that both strains adhere to Caco-2 cells and significantly upregulate the expression of autophagy-, tight junction proteins-, and anti-microbial peptides-related genes. Hence, both strains may be interesting for use as a novel functional starter culture for production of added-value yogurt with health-promoting properties.",
publisher = "MDPI, Basel",
journal = "Microorganisms",
title = "Yogurt Produced by Novel Natural Starter Cultures Improves Gut Epithelial Barrier In Vitro",
number = "10",
volume = "8",
doi = "10.3390/microorganisms8101586"
}

Popović, N., Brdarić, E., Đokić, J., Dinić, M., Veljović, K., Golić, N.,& Terzić-Vidojević, A.. (2020). Yogurt Produced by Novel Natural Starter Cultures Improves Gut Epithelial Barrier In Vitro. in Microorganisms
MDPI, Basel., 8(10).
https://doi.org/10.3390/microorganisms8101586

Popović N, Brdarić E, Đokić J, Dinić M, Veljović K, Golić N, Terzić-Vidojević A. Yogurt Produced by Novel Natural Starter Cultures Improves Gut Epithelial Barrier In Vitro. in Microorganisms. 2020;8(10).
doi:10.3390/microorganisms8101586 .

Popović, Nikola, Brdarić, Emilija, Đokić, Jelena, Dinić, Miroslav, Veljović, Katarina, Golić, Nataša, Terzić-Vidojević, Amarela, "Yogurt Produced by Novel Natural Starter Cultures Improves Gut Epithelial Barrier In Vitro" in Microorganisms, 8, no. 10 (2020),
https://doi.org/10.3390/microorganisms8101586 . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Lukić, Jovanka
AU  - Lozo, Jelena
AU  - Fira, Đorđe
AU  - Jovčić, Branko
AU  - Strahinić, Ivana
AU  - Begović, Jelena
AU  - Popović, Nikola
AU  - Miljković, Marija
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
AU  - Golić, Nataša
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1353
AB  - The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.
PB  - Elsevier, Amsterdam
T2  - Food Research International
T1  - Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties
VL  - 136
DO  - 10.1016/j.foodres.2020.109494
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Tolinački, Maja and Živković, Milica and Lukić, Jovanka and Lozo, Jelena and Fira, Đorđe and Jovčić, Branko and Strahinić, Ivana and Begović, Jelena and Popović, Nikola and Miljković, Marija and Kojić, Milan and Topisirović, Ljubiša and Golić, Nataša",
year = "2020",
abstract = "The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.",
publisher = "Elsevier, Amsterdam",
journal = "Food Research International",
title = "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties",
volume = "136",
doi = "10.1016/j.foodres.2020.109494"
}

Terzić-Vidojević, A., Veljović, K., Tolinački, M., Živković, M., Lukić, J., Lozo, J., Fira, Đ., Jovčić, B., Strahinić, I., Begović, J., Popović, N., Miljković, M., Kojić, M., Topisirović, L.,& Golić, N.. (2020). Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International
Elsevier, Amsterdam., 136.
https://doi.org/10.1016/j.foodres.2020.109494

Terzić-Vidojević A, Veljović K, Tolinački M, Živković M, Lukić J, Lozo J, Fira Đ, Jovčić B, Strahinić I, Begović J, Popović N, Miljković M, Kojić M, Topisirović L, Golić N. Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International. 2020;136.
doi:10.1016/j.foodres.2020.109494 .

Terzić-Vidojević, Amarela, Veljović, Katarina, Tolinački, Maja, Živković, Milica, Lukić, Jovanka, Lozo, Jelena, Fira, Đorđe, Jovčić, Branko, Strahinić, Ivana, Begović, Jelena, Popović, Nikola, Miljković, Marija, Kojić, Milan, Topisirović, Ljubiša, Golić, Nataša, "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties" in Food Research International, 136 (2020),
https://doi.org/10.1016/j.foodres.2020.109494 . .

TY  - CONF
AU  - Veljović, Katarina
AU  - Stanković, Marija
AU  - Soković Bajić, Svetlana
AU  - Terzić-Vidojević, Amarela
AU  - Popović, Dušanka
AU  - Dinić, Miroslav
AU  - Golić, Nataša
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1394
PB  - Lippincott Williams & Wilkins, Philadelphia
C3  - Journal of Clinical Gastroenterology
T1  - Antioxidant Activity of Lactic Acid Bacteria as Potential Probiotics in Chronic Obstructive Pulmonary Disease (COPD)
EP  - S16
SP  - S16
VL  - 54
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1394
ER  - 

@conference{
author = "Veljović, Katarina and Stanković, Marija and Soković Bajić, Svetlana and Terzić-Vidojević, Amarela and Popović, Dušanka and Dinić, Miroslav and Golić, Nataša",
year = "2020",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Clinical Gastroenterology",
title = "Antioxidant Activity of Lactic Acid Bacteria as Potential Probiotics in Chronic Obstructive Pulmonary Disease (COPD)",
pages = "S16-S16",
volume = "54",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1394"
}

Veljović, K., Stanković, M., Soković Bajić, S., Terzić-Vidojević, A., Popović, D., Dinić, M.,& Golić, N.. (2020). Antioxidant Activity of Lactic Acid Bacteria as Potential Probiotics in Chronic Obstructive Pulmonary Disease (COPD). in Journal of Clinical Gastroenterology
Lippincott Williams & Wilkins, Philadelphia., 54, S16-S16.
https://hdl.handle.net/21.15107/rcub_imagine_1394

Veljović K, Stanković M, Soković Bajić S, Terzić-Vidojević A, Popović D, Dinić M, Golić N. Antioxidant Activity of Lactic Acid Bacteria as Potential Probiotics in Chronic Obstructive Pulmonary Disease (COPD). in Journal of Clinical Gastroenterology. 2020;54:S16-S16.
https://hdl.handle.net/21.15107/rcub_imagine_1394 .

Veljović, Katarina, Stanković, Marija, Soković Bajić, Svetlana, Terzić-Vidojević, Amarela, Popović, Dušanka, Dinić, Miroslav, Golić, Nataša, "Antioxidant Activity of Lactic Acid Bacteria as Potential Probiotics in Chronic Obstructive Pulmonary Disease (COPD)" in Journal of Clinical Gastroenterology, 54 (2020):S16-S16,
https://hdl.handle.net/21.15107/rcub_imagine_1394 .

TY  - JOUR
AU  - Stanisavljević, Suzana
AU  - Cepić, Aleksa
AU  - Bojić, Svetlana
AU  - Veljović, Katarina
AU  - Mihajlović, Sanja
AU  - Dedović, Neda
AU  - Jevtić, Bojan
AU  - Momcilović, Miljana
AU  - Lazarević, Milica
AU  - Mostarica-Stojković, Marija
AU  - Miljković, Đorđe
AU  - Golić, Nataša
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1272
AB  - Gut microbiota dysbiosis has been considered the essential element in the pathogenesis of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). Antibiotics were administered orally to Dark Agouti (DA) rats early in their life with the aim of perturbing gut microbiota and investigating the effects of such intervention on the course of EAE. As a result, the diversity of the gut microbiota was reduced under the influence of antibiotics. Mainly, Firmicutes and Actinobacteria were replaced by Proteobacteria and Bacteroidetes, while decreased proportions of Clostridia and Bacilli classes were accompanied by an increase in Gamma-Proteobacteria in antibiotic-treated animals. Interestingly, a notable decrease in the Helicobacteraceae, Spirochaetaceae and Turicibacteriaceae was scored in antibiotic-treated groups. Also, levels of short chain fatty acids were reduced in the faeces of antibiotic-treated rats. Consequently, aggravation of EAE, paralleled with stronger immune response in lymph nodes draining the site of immunization, and increased inflammation within the CNS, were observed in antibiotic-treated DA rats. Thus, the alteration of gut microbiota leads to an escalation of CNS-directed autoimmunity in DA rats. The results of this study indicate that antibiotic use in early life may have subsequent unfavourable effects on the regulation of the immune system.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats
SP  - 918
VL  - 9
DO  - 10.1038/s41598-018-37505-7
ER  - 

@article{
author = "Stanisavljević, Suzana and Cepić, Aleksa and Bojić, Svetlana and Veljović, Katarina and Mihajlović, Sanja and Dedović, Neda and Jevtić, Bojan and Momcilović, Miljana and Lazarević, Milica and Mostarica-Stojković, Marija and Miljković, Đorđe and Golić, Nataša",
year = "2019",
abstract = "Gut microbiota dysbiosis has been considered the essential element in the pathogenesis of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). Antibiotics were administered orally to Dark Agouti (DA) rats early in their life with the aim of perturbing gut microbiota and investigating the effects of such intervention on the course of EAE. As a result, the diversity of the gut microbiota was reduced under the influence of antibiotics. Mainly, Firmicutes and Actinobacteria were replaced by Proteobacteria and Bacteroidetes, while decreased proportions of Clostridia and Bacilli classes were accompanied by an increase in Gamma-Proteobacteria in antibiotic-treated animals. Interestingly, a notable decrease in the Helicobacteraceae, Spirochaetaceae and Turicibacteriaceae was scored in antibiotic-treated groups. Also, levels of short chain fatty acids were reduced in the faeces of antibiotic-treated rats. Consequently, aggravation of EAE, paralleled with stronger immune response in lymph nodes draining the site of immunization, and increased inflammation within the CNS, were observed in antibiotic-treated DA rats. Thus, the alteration of gut microbiota leads to an escalation of CNS-directed autoimmunity in DA rats. The results of this study indicate that antibiotic use in early life may have subsequent unfavourable effects on the regulation of the immune system.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats",
pages = "918",
volume = "9",
doi = "10.1038/s41598-018-37505-7"
}

Stanisavljević, S., Cepić, A., Bojić, S., Veljović, K., Mihajlović, S., Dedović, N., Jevtić, B., Momcilović, M., Lazarević, M., Mostarica-Stojković, M., Miljković, Đ.,& Golić, N.. (2019). Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats. in Scientific Reports
Nature Publishing Group, London., 9, 918.
https://doi.org/10.1038/s41598-018-37505-7

Stanisavljević S, Cepić A, Bojić S, Veljović K, Mihajlović S, Dedović N, Jevtić B, Momcilović M, Lazarević M, Mostarica-Stojković M, Miljković Đ, Golić N. Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats. in Scientific Reports. 2019;9:918.
doi:10.1038/s41598-018-37505-7 .

Stanisavljević, Suzana, Cepić, Aleksa, Bojić, Svetlana, Veljović, Katarina, Mihajlović, Sanja, Dedović, Neda, Jevtić, Bojan, Momcilović, Miljana, Lazarević, Milica, Mostarica-Stojković, Marija, Miljković, Đorđe, Golić, Nataša, "Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats" in Scientific Reports, 9 (2019):918,
https://doi.org/10.1038/s41598-018-37505-7 . .

TY  - JOUR
AU  - Popović, Nikola
AU  - Đokić, Jelena
AU  - Brdarić, Emilija
AU  - Dinić, Miroslav
AU  - Terzić-Vidojević, Amarela
AU  - Golić, Nataša
AU  - Veljović, Katarina
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1279
AB  - Listeria monocytogenes, the common foodborne pathogenic bacteria species, compromises the intestinal epithelial barrier, leading to development of the listeriosis, a severe disease especially among immunocompromised individuals. L. monocytogenes infection usually requires antibiotic treatment, however, excessive use of antibiotics promotes emergence of antibiotic resistance and the destruction of gut microbiota. Probiotics, including lactic acid bacteria (LAB), have been repeatedly proven as an alternative approach for the treatment of various infections. We have analyzed the potential of Enterococcus faecium BGPAS1-3, a dairy isolate exhibiting strong direct antilisterial effect, to modulate the response of differentiated Caco-2 intestinal epithelial cells to L. monocytogenes ATCC 19111 infection. We showed that the molecule with antilisterial effect is a bacterial cell-wall protein that is highly resistant to the high-temperature treatment. When we tested the antilisterial potential of heat-killed BGPAS1-3, we found that it could prevent tight junction disruption in differentiated Caco-2 monolayer infected with L. monocytogenes ATCC 19111, induce antilisterial host response mechanisms, and stimulate the production of protective TGF-beta in intestinal epithelial cells. We also showed that the modulation of MyD88 dependent TLR2 and TLR4 pathways by BGPAS1-3 are involved in host response against L. monocytogenes ATCC 19111. Since heat-killed BGPAS1-3 possess strong antilisterial effects, such postbiotic could be used as a controllable and safe therapeutic.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111
VL  - 10
DO  - 10.3389/fmicb.2019.00412
ER  - 

@article{
author = "Popović, Nikola and Đokić, Jelena and Brdarić, Emilija and Dinić, Miroslav and Terzić-Vidojević, Amarela and Golić, Nataša and Veljović, Katarina",
year = "2019",
abstract = "Listeria monocytogenes, the common foodborne pathogenic bacteria species, compromises the intestinal epithelial barrier, leading to development of the listeriosis, a severe disease especially among immunocompromised individuals. L. monocytogenes infection usually requires antibiotic treatment, however, excessive use of antibiotics promotes emergence of antibiotic resistance and the destruction of gut microbiota. Probiotics, including lactic acid bacteria (LAB), have been repeatedly proven as an alternative approach for the treatment of various infections. We have analyzed the potential of Enterococcus faecium BGPAS1-3, a dairy isolate exhibiting strong direct antilisterial effect, to modulate the response of differentiated Caco-2 intestinal epithelial cells to L. monocytogenes ATCC 19111 infection. We showed that the molecule with antilisterial effect is a bacterial cell-wall protein that is highly resistant to the high-temperature treatment. When we tested the antilisterial potential of heat-killed BGPAS1-3, we found that it could prevent tight junction disruption in differentiated Caco-2 monolayer infected with L. monocytogenes ATCC 19111, induce antilisterial host response mechanisms, and stimulate the production of protective TGF-beta in intestinal epithelial cells. We also showed that the modulation of MyD88 dependent TLR2 and TLR4 pathways by BGPAS1-3 are involved in host response against L. monocytogenes ATCC 19111. Since heat-killed BGPAS1-3 possess strong antilisterial effects, such postbiotic could be used as a controllable and safe therapeutic.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111",
volume = "10",
doi = "10.3389/fmicb.2019.00412"
}

Popović, N., Đokić, J., Brdarić, E., Dinić, M., Terzić-Vidojević, A., Golić, N.,& Veljović, K.. (2019). The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fmicb.2019.00412

Popović N, Đokić J, Brdarić E, Dinić M, Terzić-Vidojević A, Golić N, Veljović K. The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111. in Frontiers in Microbiology. 2019;10.
doi:10.3389/fmicb.2019.00412 .

Popović, Nikola, Đokić, Jelena, Brdarić, Emilija, Dinić, Miroslav, Terzić-Vidojević, Amarela, Golić, Nataša, Veljović, Katarina, "The Influence of Heat-Killed Enterococcus faecium BGPAS1-3 on the Tight Junction Protein Expression and Immune Function in Differentiated Caco-2 Cells Infected With Listeria monocytogenes ATCC 19111" in Frontiers in Microbiology, 10 (2019),
https://doi.org/10.3389/fmicb.2019.00412 . .

TY  - JOUR
AU  - Soković Bajić, Svetlana
AU  - Đokić, Jelena
AU  - Dinić, Miroslav
AU  - Veljović, Katarina
AU  - Golić, Nataša
AU  - Mihajlović, Sanja
AU  - Tolinački, Maja
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1298
AB  - Probiotic bacteria are recognized for their health-promoting properties, including maintenance of gut epithelial integrity and host immune system homeostasis. Taking into account the beneficial health-promoting effects of GABA, the presence of the gadB gene, encoding glutamate decarboxylase that converts L-glutamate to GABA, was analyzed in Lactic Acid Bacteria (LAB) natural isolates from Zlatar cheese. The results revealed that 52% of tested Lactobacillus spp. and 8% of Lactococcus spp. isolates harbor the gadB gene. Qualitative and quantitative analysis of GABA production performed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) revealed the highest GABA production by Lactobacillus brevis BGZLS10-17. Since high GABA-producing LAB natural isolates are the most valuable source of naturally produced GABA, the probiotic properties of BGZLS10-17 were characterized. This study demonstrated high adhesion of BGZLS10-17 strain to Caco2 cells and the ability to decrease the adhesion of Escherichia coli ATCC25922 and Salmonella enterica C29039. Treatment of differentiated Caco-2 cells monolayer with BGZLS10-17 supernatant containing GABA alleviated inflammation (production of IL-8) caused by IL-1 beta and significantly stimulated the expression of tight junction proteins (zonulin, occludin, and claudin 4), as well as the expression of TGF-b cytokine leading to the conclusion that immunosuppression and strengthening the tight junctions can have significant role in the maintenance of intestinal epithelial barrier integrity. Taken together the results obtained in this study support the idea that using of GABA producing BGZLS10-17 probiotic strain could be a good strategy to modulate immunological response in various inflammatory diseases, and at the same time, it could be a good candidate for adjunct starter culture for production of GABA-enriched dairy foods and beverages offering new perspectives in designing the novel functional foods.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - GABA-Producing Natural Dairy Isolate From Artisanal Zlatar Cheese Attenuates Gut Inflammation and Strengthens Gut Epithelial Barrier in vitro
VL  - 10
DO  - 10.3389/fmicb.2019.00527
ER  - 

@article{
author = "Soković Bajić, Svetlana and Đokić, Jelena and Dinić, Miroslav and Veljović, Katarina and Golić, Nataša and Mihajlović, Sanja and Tolinački, Maja",
year = "2019",
abstract = "Probiotic bacteria are recognized for their health-promoting properties, including maintenance of gut epithelial integrity and host immune system homeostasis. Taking into account the beneficial health-promoting effects of GABA, the presence of the gadB gene, encoding glutamate decarboxylase that converts L-glutamate to GABA, was analyzed in Lactic Acid Bacteria (LAB) natural isolates from Zlatar cheese. The results revealed that 52% of tested Lactobacillus spp. and 8% of Lactococcus spp. isolates harbor the gadB gene. Qualitative and quantitative analysis of GABA production performed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) revealed the highest GABA production by Lactobacillus brevis BGZLS10-17. Since high GABA-producing LAB natural isolates are the most valuable source of naturally produced GABA, the probiotic properties of BGZLS10-17 were characterized. This study demonstrated high adhesion of BGZLS10-17 strain to Caco2 cells and the ability to decrease the adhesion of Escherichia coli ATCC25922 and Salmonella enterica C29039. Treatment of differentiated Caco-2 cells monolayer with BGZLS10-17 supernatant containing GABA alleviated inflammation (production of IL-8) caused by IL-1 beta and significantly stimulated the expression of tight junction proteins (zonulin, occludin, and claudin 4), as well as the expression of TGF-b cytokine leading to the conclusion that immunosuppression and strengthening the tight junctions can have significant role in the maintenance of intestinal epithelial barrier integrity. Taken together the results obtained in this study support the idea that using of GABA producing BGZLS10-17 probiotic strain could be a good strategy to modulate immunological response in various inflammatory diseases, and at the same time, it could be a good candidate for adjunct starter culture for production of GABA-enriched dairy foods and beverages offering new perspectives in designing the novel functional foods.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "GABA-Producing Natural Dairy Isolate From Artisanal Zlatar Cheese Attenuates Gut Inflammation and Strengthens Gut Epithelial Barrier in vitro",
volume = "10",
doi = "10.3389/fmicb.2019.00527"
}

Soković Bajić, S., Đokić, J., Dinić, M., Veljović, K., Golić, N., Mihajlović, S.,& Tolinački, M.. (2019). GABA-Producing Natural Dairy Isolate From Artisanal Zlatar Cheese Attenuates Gut Inflammation and Strengthens Gut Epithelial Barrier in vitro. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fmicb.2019.00527

Soković Bajić S, Đokić J, Dinić M, Veljović K, Golić N, Mihajlović S, Tolinački M. GABA-Producing Natural Dairy Isolate From Artisanal Zlatar Cheese Attenuates Gut Inflammation and Strengthens Gut Epithelial Barrier in vitro. in Frontiers in Microbiology. 2019;10.
doi:10.3389/fmicb.2019.00527 .

Soković Bajić, Svetlana, Đokić, Jelena, Dinić, Miroslav, Veljović, Katarina, Golić, Nataša, Mihajlović, Sanja, Tolinački, Maja, "GABA-Producing Natural Dairy Isolate From Artisanal Zlatar Cheese Attenuates Gut Inflammation and Strengthens Gut Epithelial Barrier in vitro" in Frontiers in Microbiology, 10 (2019),
https://doi.org/10.3389/fmicb.2019.00527 . .

TY  - JOUR
AU  - Kulas, Jelena
AU  - Mirkov, Ivana
AU  - Tucović, Dina
AU  - Zolotarevski, Lidija
AU  - Glamoclija, Jasmina
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Golić, Nataša
AU  - Kataranovski, Milena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1299
AB  - Microbiota inhabiting mucosal tissues is involved in maintenance of their immune homeostasis. Growing body of evidence indicate that dysbiosis in gut influence immune responses at distal sites including lungs. There are also reports concerning gut involvement with pulmonary injury/inflammation in settings of respiratory viral and bacterial infections. The impact of infections with other microorganisms on gut homeostasis is not explored. In this study, the rat model of sublethal pulmonary infection with Aspergillus fumigants was used to investigate the effect of fungal respiratory infection on gut immune-mediated homeostasis. Signs of intestinal damage, intestinal and gut-draining lymphoid tissue cytokine responses and gut bacterial microbiota diversity were examined. Intestinal injury, inflammatory cell infiltration, as well as increased levels of intestinal interferon-gamma (IFN-gamma) and interleukin-17 (IL-17) (as opposed to unchanged levels of anti-inflammatory cytokine IL-10) during the two-week period depict intestinal inflammation in rats with pulmonary A. fumigates infection. It could not be ascribed to the fungus as it was not detected in the intestine of infected rats. Increased production of pro-inflammatory cytokines by major gut-draining mesenteric lymph nodes point to these lymphoid organs as places of generation of cytokine-producing cells. No changes in spleen or systemic cytokine responses was observed, showing lack of the effects of pulmonary A. fumigatus infection outside mucosal immune system. Drop of intestinal bacterial microbiota diversity (disappearance of several bacterial bands) was noted early in infection with normalization starting from day seven. From day three, appearance of new bacterial bands (unique to infected individuals, not present in controls) was seen, and some of them are pathogens. Alterations in intestinal bacterial community might have affected intestinal immune tolerance contributing to inflammation. Disruption of gut homeostasis during pulmonary infection might render gastrointestinal tract more susceptible to variety of physiological and pathological stimuli. Data which showed for the first time gut involvement with pulmonary infection with A. fumigatus provide the baseline for future studies of the impact of fungal lung infections to gut homeostasis, particularly in individuals susceptible to these infections.
PB  - Elsevier Gmbh, Munich
T2  - Immunobiology
T1  - Pulmonary Aspergillus fumigatus infection in rats affects gastrointestinal homeostasis
EP  - 123
IS  - 1
SP  - 116
VL  - 224
DO  - 10.1016/j.imbio.2018.10.001
ER  - 

@article{
author = "Kulas, Jelena and Mirkov, Ivana and Tucović, Dina and Zolotarevski, Lidija and Glamoclija, Jasmina and Veljović, Katarina and Tolinački, Maja and Golić, Nataša and Kataranovski, Milena",
year = "2019",
abstract = "Microbiota inhabiting mucosal tissues is involved in maintenance of their immune homeostasis. Growing body of evidence indicate that dysbiosis in gut influence immune responses at distal sites including lungs. There are also reports concerning gut involvement with pulmonary injury/inflammation in settings of respiratory viral and bacterial infections. The impact of infections with other microorganisms on gut homeostasis is not explored. In this study, the rat model of sublethal pulmonary infection with Aspergillus fumigants was used to investigate the effect of fungal respiratory infection on gut immune-mediated homeostasis. Signs of intestinal damage, intestinal and gut-draining lymphoid tissue cytokine responses and gut bacterial microbiota diversity were examined. Intestinal injury, inflammatory cell infiltration, as well as increased levels of intestinal interferon-gamma (IFN-gamma) and interleukin-17 (IL-17) (as opposed to unchanged levels of anti-inflammatory cytokine IL-10) during the two-week period depict intestinal inflammation in rats with pulmonary A. fumigates infection. It could not be ascribed to the fungus as it was not detected in the intestine of infected rats. Increased production of pro-inflammatory cytokines by major gut-draining mesenteric lymph nodes point to these lymphoid organs as places of generation of cytokine-producing cells. No changes in spleen or systemic cytokine responses was observed, showing lack of the effects of pulmonary A. fumigatus infection outside mucosal immune system. Drop of intestinal bacterial microbiota diversity (disappearance of several bacterial bands) was noted early in infection with normalization starting from day seven. From day three, appearance of new bacterial bands (unique to infected individuals, not present in controls) was seen, and some of them are pathogens. Alterations in intestinal bacterial community might have affected intestinal immune tolerance contributing to inflammation. Disruption of gut homeostasis during pulmonary infection might render gastrointestinal tract more susceptible to variety of physiological and pathological stimuli. Data which showed for the first time gut involvement with pulmonary infection with A. fumigatus provide the baseline for future studies of the impact of fungal lung infections to gut homeostasis, particularly in individuals susceptible to these infections.",
publisher = "Elsevier Gmbh, Munich",
journal = "Immunobiology",
title = "Pulmonary Aspergillus fumigatus infection in rats affects gastrointestinal homeostasis",
pages = "123-116",
number = "1",
volume = "224",
doi = "10.1016/j.imbio.2018.10.001"
}

Kulas, J., Mirkov, I., Tucović, D., Zolotarevski, L., Glamoclija, J., Veljović, K., Tolinački, M., Golić, N.,& Kataranovski, M.. (2019). Pulmonary Aspergillus fumigatus infection in rats affects gastrointestinal homeostasis. in Immunobiology
Elsevier Gmbh, Munich., 224(1), 116-123.
https://doi.org/10.1016/j.imbio.2018.10.001

Kulas J, Mirkov I, Tucović D, Zolotarevski L, Glamoclija J, Veljović K, Tolinački M, Golić N, Kataranovski M. Pulmonary Aspergillus fumigatus infection in rats affects gastrointestinal homeostasis. in Immunobiology. 2019;224(1):116-123.
doi:10.1016/j.imbio.2018.10.001 .

Kulas, Jelena, Mirkov, Ivana, Tucović, Dina, Zolotarevski, Lidija, Glamoclija, Jasmina, Veljović, Katarina, Tolinački, Maja, Golić, Nataša, Kataranovski, Milena, "Pulmonary Aspergillus fumigatus infection in rats affects gastrointestinal homeostasis" in Immunobiology, 224, no. 1 (2019):116-123,
https://doi.org/10.1016/j.imbio.2018.10.001 . .

TY  - JOUR
AU  - Popović, Nikola
AU  - Dinić, Miroslav
AU  - Tolinački, Maja
AU  - Mihajlović, Sanja
AU  - Terzić-Vidojević, Amarela
AU  - Bojić, Svetlana
AU  - Đokić, Jelena
AU  - Golić, Nataša
AU  - Veljović, Katarina
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1157
AB  - Enterococci have controversial status due to their emerging role in nosocomial infections and transmission of antibiotic resistance genes, while some enterococci strains are used as probiotics for humans and animals and starter cultures in dairy industry. In order to improve our understanding of factors involved in the safe use of enterococci as potential probiotics, the antibiotic susceptibility, virulence and probiotic traits of 75 dairy enterococci isolates belonging to Enterococcus durans (50), En. faeciurn (15), En. faecalis (6), En. italious (3), and En, hirae (1) were evaluated. The results revealed that ciprofloxacin resistance and biofilm formation are correlated with isolates originated from Golija mountain (Serbia), while gelatinase activity was more common in isolates from Prigorje region (Croatia), pointing to uncontrolled use of antibiotics and anthropogenic impact on dairy products" microbiota in these regions. The virulence genes were sporadically present in 13 selected dairy enterococci isolates. Interestingly, biofilm formation was correlated with higher ability of strains to reduce the adhesion of E. coil and Salmonella Enteritidis to HT29-MTX cells. To our knowledge this is the first study reporting the presence of the esp gene (previously correlated with pathogenesis) in dairy enterococci isolates, mostly associated with the genes involved in adhesion property. Hence, the results of this study revealed that the virulence genes are sporadically present in dairy isolates and more correlated to adhesion properties and biofilm formation, implicating their role in gut colonization rather than to the virulence traits.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates
VL  - 9
DO  - 10.3389/fmicb.2018.00078
ER  - 

@article{
author = "Popović, Nikola and Dinić, Miroslav and Tolinački, Maja and Mihajlović, Sanja and Terzić-Vidojević, Amarela and Bojić, Svetlana and Đokić, Jelena and Golić, Nataša and Veljović, Katarina",
year = "2018",
abstract = "Enterococci have controversial status due to their emerging role in nosocomial infections and transmission of antibiotic resistance genes, while some enterococci strains are used as probiotics for humans and animals and starter cultures in dairy industry. In order to improve our understanding of factors involved in the safe use of enterococci as potential probiotics, the antibiotic susceptibility, virulence and probiotic traits of 75 dairy enterococci isolates belonging to Enterococcus durans (50), En. faeciurn (15), En. faecalis (6), En. italious (3), and En, hirae (1) were evaluated. The results revealed that ciprofloxacin resistance and biofilm formation are correlated with isolates originated from Golija mountain (Serbia), while gelatinase activity was more common in isolates from Prigorje region (Croatia), pointing to uncontrolled use of antibiotics and anthropogenic impact on dairy products" microbiota in these regions. The virulence genes were sporadically present in 13 selected dairy enterococci isolates. Interestingly, biofilm formation was correlated with higher ability of strains to reduce the adhesion of E. coil and Salmonella Enteritidis to HT29-MTX cells. To our knowledge this is the first study reporting the presence of the esp gene (previously correlated with pathogenesis) in dairy enterococci isolates, mostly associated with the genes involved in adhesion property. Hence, the results of this study revealed that the virulence genes are sporadically present in dairy isolates and more correlated to adhesion properties and biofilm formation, implicating their role in gut colonization rather than to the virulence traits.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates",
volume = "9",
doi = "10.3389/fmicb.2018.00078"
}

Popović, N., Dinić, M., Tolinački, M., Mihajlović, S., Terzić-Vidojević, A., Bojić, S., Đokić, J., Golić, N.,& Veljović, K.. (2018). New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fmicb.2018.00078

Popović N, Dinić M, Tolinački M, Mihajlović S, Terzić-Vidojević A, Bojić S, Đokić J, Golić N, Veljović K. New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates. in Frontiers in Microbiology. 2018;9.
doi:10.3389/fmicb.2018.00078 .

Popović, Nikola, Dinić, Miroslav, Tolinački, Maja, Mihajlović, Sanja, Terzić-Vidojević, Amarela, Bojić, Svetlana, Đokić, Jelena, Golić, Nataša, Veljović, Katarina, "New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp Dairy Isolates" in Frontiers in Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.00078 . .

TY  - JOUR
AU  - Stanojević, Stanislava
AU  - Blagojević, Veljko
AU  - Curuvija, Ivana
AU  - Veljović, Katarina
AU  - Soković Bajić, Svetlana
AU  - Kotur-Stevuljević, Jelena
AU  - Bogdanović, Andrija
AU  - Petrović, Raisa
AU  - Vujnović, Ivana
AU  - Kovacević-Jovanović, Vesna
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1149
AB  - The current study investigated a potential modulating effect of orally applied Lactobacillus rhamnosus 64 (LB64) during the early postnatal period (day of life: similar to 3-30), during young adult period (day of life: 31-70) or throughout experiment, on parameters of trinitrobenzenesulfonic acid (TNBS)-induced colitis in adult rats. Treatment with LB64 during early postnatal, but not during young adult period reduced clinical damage score, neutrophil and macrophage infiltration into colon, the level of cytokine and myeloperoxidase (MPO) activity, but had no influence on other parameters of oxidative damage. Early postnatal treatment with LB64 also increased the diversity of fecal Bifidobacteria and Eubacteria, and improved maturation of ileal villi in 30-days old rats. When LB64 is applied during a critical period early in life, it affects immune system functioning of adults, probably by interactions with the mucosal immune system of the gastrointestinal tract that provides immune system maturation and shapes the overall immune response.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Functional Foods
T1  - Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis
EP  - 105
SP  - 92
VL  - 48
DO  - 10.1016/j.jff.2018.07.014
ER  - 

@article{
author = "Stanojević, Stanislava and Blagojević, Veljko and Curuvija, Ivana and Veljović, Katarina and Soković Bajić, Svetlana and Kotur-Stevuljević, Jelena and Bogdanović, Andrija and Petrović, Raisa and Vujnović, Ivana and Kovacević-Jovanović, Vesna",
year = "2018",
abstract = "The current study investigated a potential modulating effect of orally applied Lactobacillus rhamnosus 64 (LB64) during the early postnatal period (day of life: similar to 3-30), during young adult period (day of life: 31-70) or throughout experiment, on parameters of trinitrobenzenesulfonic acid (TNBS)-induced colitis in adult rats. Treatment with LB64 during early postnatal, but not during young adult period reduced clinical damage score, neutrophil and macrophage infiltration into colon, the level of cytokine and myeloperoxidase (MPO) activity, but had no influence on other parameters of oxidative damage. Early postnatal treatment with LB64 also increased the diversity of fecal Bifidobacteria and Eubacteria, and improved maturation of ileal villi in 30-days old rats. When LB64 is applied during a critical period early in life, it affects immune system functioning of adults, probably by interactions with the mucosal immune system of the gastrointestinal tract that provides immune system maturation and shapes the overall immune response.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Functional Foods",
title = "Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis",
pages = "105-92",
volume = "48",
doi = "10.1016/j.jff.2018.07.014"
}

Stanojević, S., Blagojević, V., Curuvija, I., Veljović, K., Soković Bajić, S., Kotur-Stevuljević, J., Bogdanović, A., Petrović, R., Vujnović, I.,& Kovacević-Jovanović, V.. (2018). Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis. in Journal of Functional Foods
Elsevier Science Bv, Amsterdam., 48, 92-105.
https://doi.org/10.1016/j.jff.2018.07.014

Stanojević S, Blagojević V, Curuvija I, Veljović K, Soković Bajić S, Kotur-Stevuljević J, Bogdanović A, Petrović R, Vujnović I, Kovacević-Jovanović V. Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis. in Journal of Functional Foods. 2018;48:92-105.
doi:10.1016/j.jff.2018.07.014 .

Stanojević, Stanislava, Blagojević, Veljko, Curuvija, Ivana, Veljović, Katarina, Soković Bajić, Svetlana, Kotur-Stevuljević, Jelena, Bogdanović, Andrija, Petrović, Raisa, Vujnović, Ivana, Kovacević-Jovanović, Vesna, "Oral treatment with Lactobacillus rhamnosus 64 during the early postnatal period improves the health of adult rats with TNBS-induced colitis" in Journal of Functional Foods, 48 (2018):92-105,
https://doi.org/10.1016/j.jff.2018.07.014 . .

TY  - JOUR
AU  - Veljović, Katarina
AU  - Dinić, Miroslav
AU  - Lukić, Jovanka
AU  - Mihajlović, Sanja
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Begović, Jelena
AU  - Mrvaljević, Igor
AU  - Golić, Nataša
AU  - Terzić-Vidojević, Amarela
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1031
AB  - The aim of this work was to design a novel mixed probiotic culture for piglets and to evaluate its beneficial effect on the piglets' gut health. The possible mechanisms of probiotic activity, such as adhesion, competitive pathogen exclusion and influence on gut microbiota diversity were determined. Mixed probiotic starter culture is composed of three thermophilic lactic acid bacteria (LAB) strains: Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44. The strains BGVLJ1-44 and BGRA43 showed good technological properties (fast milk curdling, strong proteolytic activity). In addition, the strain BGVLJ1-44 produces exopolysaccharide (EPS), BGHI14 is heterofermentative LAB strain with significant immunomodulatory effect, while the strain BGRA43 showed strong antimicrobial activity against different pathogens and exhibited significantly higher level of adhesion to Caco-2 cells comparing to other two strains. Both lactobacilli strains BGRA43 and BGHI14 (p  lt  0.05), as well as probiotic combination (p  lt  0.01) significantly reduced the adhesion of Escherichia coli ATCC25922 to Caco-2 cells, while the strains BGVLJ1-44 (p  lt  0.01) and BGRA43 (p  lt  0.05) significantly reduced adhesion of Salmonella 654/7E (veterinary isolate). The results of farm trial revealed that treatment of sows with new fermented dairy probiotic influenced the piglets' gut colonization with beneficial bacteria and reduced the number of enterobacteriaceae in litters from some treated sows (no significant due to high variability among animals). Finally, this is the first study reporting that the treatment of sows with probiotic combination resulted in the improved microbiota diversity in neonatal piglets.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows
VL  - 8
DO  - 10.3389/fmicb.2017.02028
ER  - 

@article{
author = "Veljović, Katarina and Dinić, Miroslav and Lukić, Jovanka and Mihajlović, Sanja and Tolinački, Maja and Živković, Milica and Begović, Jelena and Mrvaljević, Igor and Golić, Nataša and Terzić-Vidojević, Amarela",
year = "2017",
abstract = "The aim of this work was to design a novel mixed probiotic culture for piglets and to evaluate its beneficial effect on the piglets' gut health. The possible mechanisms of probiotic activity, such as adhesion, competitive pathogen exclusion and influence on gut microbiota diversity were determined. Mixed probiotic starter culture is composed of three thermophilic lactic acid bacteria (LAB) strains: Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44. The strains BGVLJ1-44 and BGRA43 showed good technological properties (fast milk curdling, strong proteolytic activity). In addition, the strain BGVLJ1-44 produces exopolysaccharide (EPS), BGHI14 is heterofermentative LAB strain with significant immunomodulatory effect, while the strain BGRA43 showed strong antimicrobial activity against different pathogens and exhibited significantly higher level of adhesion to Caco-2 cells comparing to other two strains. Both lactobacilli strains BGRA43 and BGHI14 (p  lt  0.05), as well as probiotic combination (p  lt  0.01) significantly reduced the adhesion of Escherichia coli ATCC25922 to Caco-2 cells, while the strains BGVLJ1-44 (p  lt  0.01) and BGRA43 (p  lt  0.05) significantly reduced adhesion of Salmonella 654/7E (veterinary isolate). The results of farm trial revealed that treatment of sows with new fermented dairy probiotic influenced the piglets' gut colonization with beneficial bacteria and reduced the number of enterobacteriaceae in litters from some treated sows (no significant due to high variability among animals). Finally, this is the first study reporting that the treatment of sows with probiotic combination resulted in the improved microbiota diversity in neonatal piglets.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows",
volume = "8",
doi = "10.3389/fmicb.2017.02028"
}

Veljović, K., Dinić, M., Lukić, J., Mihajlović, S., Tolinački, M., Živković, M., Begović, J., Mrvaljević, I., Golić, N.,& Terzić-Vidojević, A.. (2017). Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fmicb.2017.02028

Veljović K, Dinić M, Lukić J, Mihajlović S, Tolinački M, Živković M, Begović J, Mrvaljević I, Golić N, Terzić-Vidojević A. Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows. in Frontiers in Microbiology. 2017;8.
doi:10.3389/fmicb.2017.02028 .

Veljović, Katarina, Dinić, Miroslav, Lukić, Jovanka, Mihajlović, Sanja, Tolinački, Maja, Živković, Milica, Begović, Jelena, Mrvaljević, Igor, Golić, Nataša, Terzić-Vidojević, Amarela, "Promotion of Early Gut Colonization by Probiotic Intervention on Microbiota Diversity in Pregnant Sows" in Frontiers in Microbiology, 8 (2017),
https://doi.org/10.3389/fmicb.2017.02028 . .

TY  - JOUR
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Miljković, Marija
AU  - Tolinački, Maja
AU  - Terzić-Vidojević, Amarela
AU  - Kojić, Milan
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/997
AB  - The understanding of mechanisms of interactions between various bacterial cell surface proteins and host receptors has become imperative for the study of the health promoting features of probiotic enterococci. This study, for the first time, describes a novel enterococcal aggregation protein, AggE, from Enterococcus faeciurn BGGO9-28, selected from a laboratory collection of enterococcal isolates with auto aggregation phenotypes. Among them, En. faecium BGG09-28 showed the strongest auto -aggregation, adhesion to components of ECM and biofilm formation. Novel aggregation promoting factor AggE, a protein of 178.1 kDa, belongs to the collagen -binding superfamily of proteins and shares similar architecture with previously discovered aggregation factors from lactic acid bacteria (LAB). Its expression in heterologous enterococcal and lactococcal hosts demonstrates that the aggE gene is sufficient for cell aggregation. The derivatives carrying aggE exhibited the ten times higher adhesion ability to collagen and fibronectin, possess about two times higher adhesion to mucin and contribute to the increase of biofilm formation, comparing to the control strains. Analysis for the presence of virulence factors (cytolysin and gelatinase production), antibiotic resistance (antibiotic susceptibility) and genes (cylA, egg, gelE, esp, hyiN, ace, efaks, and efagn) showed that BGG09-28 was sensitive to all tested antibiotics, without hemolytic or gelatinase activity. This strain does not carry any of the tested genes encoding for known virulence factors. Results showed that BGGO9-28 was resistant to low pH and high concentrations of bile salts. Also, it adhered strongly to the Caco-2 human epithelial cell line. In conclusion, the results of this study indicate that the presence of AggE protein on the cell surface in enterococci is a desirable probiotic feature.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Novel Aggregation Promoting Factor AggE Contributes to the Probiotic Properties of Enterococcus faecium BGGO9-28
VL  - 8
DO  - 10.3389/fmicb.2017.01843
ER  - 

@article{
author = "Veljović, Katarina and Popović, Nikola and Miljković, Marija and Tolinački, Maja and Terzić-Vidojević, Amarela and Kojić, Milan",
year = "2017",
abstract = "The understanding of mechanisms of interactions between various bacterial cell surface proteins and host receptors has become imperative for the study of the health promoting features of probiotic enterococci. This study, for the first time, describes a novel enterococcal aggregation protein, AggE, from Enterococcus faeciurn BGGO9-28, selected from a laboratory collection of enterococcal isolates with auto aggregation phenotypes. Among them, En. faecium BGG09-28 showed the strongest auto -aggregation, adhesion to components of ECM and biofilm formation. Novel aggregation promoting factor AggE, a protein of 178.1 kDa, belongs to the collagen -binding superfamily of proteins and shares similar architecture with previously discovered aggregation factors from lactic acid bacteria (LAB). Its expression in heterologous enterococcal and lactococcal hosts demonstrates that the aggE gene is sufficient for cell aggregation. The derivatives carrying aggE exhibited the ten times higher adhesion ability to collagen and fibronectin, possess about two times higher adhesion to mucin and contribute to the increase of biofilm formation, comparing to the control strains. Analysis for the presence of virulence factors (cytolysin and gelatinase production), antibiotic resistance (antibiotic susceptibility) and genes (cylA, egg, gelE, esp, hyiN, ace, efaks, and efagn) showed that BGG09-28 was sensitive to all tested antibiotics, without hemolytic or gelatinase activity. This strain does not carry any of the tested genes encoding for known virulence factors. Results showed that BGGO9-28 was resistant to low pH and high concentrations of bile salts. Also, it adhered strongly to the Caco-2 human epithelial cell line. In conclusion, the results of this study indicate that the presence of AggE protein on the cell surface in enterococci is a desirable probiotic feature.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Novel Aggregation Promoting Factor AggE Contributes to the Probiotic Properties of Enterococcus faecium BGGO9-28",
volume = "8",
doi = "10.3389/fmicb.2017.01843"
}

Veljović, K., Popović, N., Miljković, M., Tolinački, M., Terzić-Vidojević, A.,& Kojić, M.. (2017). Novel Aggregation Promoting Factor AggE Contributes to the Probiotic Properties of Enterococcus faecium BGGO9-28. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fmicb.2017.01843

Veljović K, Popović N, Miljković M, Tolinački M, Terzić-Vidojević A, Kojić M. Novel Aggregation Promoting Factor AggE Contributes to the Probiotic Properties of Enterococcus faecium BGGO9-28. in Frontiers in Microbiology. 2017;8.
doi:10.3389/fmicb.2017.01843 .

Veljović, Katarina, Popović, Nikola, Miljković, Marija, Tolinački, Maja, Terzić-Vidojević, Amarela, Kojić, Milan, "Novel Aggregation Promoting Factor AggE Contributes to the Probiotic Properties of Enterococcus faecium BGGO9-28" in Frontiers in Microbiology, 8 (2017),
https://doi.org/10.3389/fmicb.2017.01843 . .

TY  - JOUR
AU  - Golić, Nataša
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Đokić, Jelena
AU  - Strahinić, Ivana
AU  - Mrvaljević, Igor
AU  - Terzić-Vidojević, Amarela
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1091
AB  - Background: Genus Clostridium accompanies more than 200 known species and at least 30 among them are associated with human and animal diseases. At the moment, the treatment of clostridial infections is based on use of antibiotics. However, due to the European ban on the use of antibiotics in livestock production, novel therapeutic strategies for treatment of these hardly curable infections have been evaluated. Hence, in this study the antimicrobial effect of newly designed probiotic culture consisted of natural isolates Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44 against Clostridium difficile and Clostridium perfringens was analyzed. Results: The probiotic culture showed strong in vitro antimicrobial effect on C. difficile (human clinical isolate). In addition, individual strains and the probiotic combination exhibited immunomodulatory activity. The probiotic combination significantly increased the proliferation of GALT lymphocytes. At the other hand, none of the bacterial treatments (individual strains and the combination) induced the production of proinflammatory cytokines IL-6 and IL-1 beta by intestinal epithelial cells, Caco-2. Interestingly, Caco-2 cells exposed to the probiotic combination produced significantly elevated amount of TGF beta pointing to potential protecting effect of the probiotic. In addition, the results of field trial on spontaneously infected goats revealed reduction of C. perfringens in goats (below the detection threshold) after the probiotic treatment. Conclusions: The results of this study indicated that the novel probiotic deserves to be further investigated as a promising antimicrobial agent against C. difficile and C. perfringens.
PB  - BMC, London
T2  - BMC Microbiology
T1  - In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens
VL  - 17
DO  - 10.1186/s12866-017-1015-5
ER  - 

@article{
author = "Golić, Nataša and Veljović, Katarina and Popović, Nikola and Đokić, Jelena and Strahinić, Ivana and Mrvaljević, Igor and Terzić-Vidojević, Amarela",
year = "2017",
abstract = "Background: Genus Clostridium accompanies more than 200 known species and at least 30 among them are associated with human and animal diseases. At the moment, the treatment of clostridial infections is based on use of antibiotics. However, due to the European ban on the use of antibiotics in livestock production, novel therapeutic strategies for treatment of these hardly curable infections have been evaluated. Hence, in this study the antimicrobial effect of newly designed probiotic culture consisted of natural isolates Lactobacillus helveticus BGRA43, Lactobacillus fermentum BGHI14 and Streptococcus thermophilus BGVLJ1-44 against Clostridium difficile and Clostridium perfringens was analyzed. Results: The probiotic culture showed strong in vitro antimicrobial effect on C. difficile (human clinical isolate). In addition, individual strains and the probiotic combination exhibited immunomodulatory activity. The probiotic combination significantly increased the proliferation of GALT lymphocytes. At the other hand, none of the bacterial treatments (individual strains and the combination) induced the production of proinflammatory cytokines IL-6 and IL-1 beta by intestinal epithelial cells, Caco-2. Interestingly, Caco-2 cells exposed to the probiotic combination produced significantly elevated amount of TGF beta pointing to potential protecting effect of the probiotic. In addition, the results of field trial on spontaneously infected goats revealed reduction of C. perfringens in goats (below the detection threshold) after the probiotic treatment. Conclusions: The results of this study indicated that the novel probiotic deserves to be further investigated as a promising antimicrobial agent against C. difficile and C. perfringens.",
publisher = "BMC, London",
journal = "BMC Microbiology",
title = "In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens",
volume = "17",
doi = "10.1186/s12866-017-1015-5"
}

Golić, N., Veljović, K., Popović, N., Đokić, J., Strahinić, I., Mrvaljević, I.,& Terzić-Vidojević, A.. (2017). In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens. in BMC Microbiology
BMC, London., 17.
https://doi.org/10.1186/s12866-017-1015-5

Golić N, Veljović K, Popović N, Đokić J, Strahinić I, Mrvaljević I, Terzić-Vidojević A. In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens. in BMC Microbiology. 2017;17.
doi:10.1186/s12866-017-1015-5 .

Golić, Nataša, Veljović, Katarina, Popović, Nikola, Đokić, Jelena, Strahinić, Ivana, Mrvaljević, Igor, Terzić-Vidojević, Amarela, "In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens" in BMC Microbiology, 17 (2017),
https://doi.org/10.1186/s12866-017-1015-5 . .

TY  - JOUR
AU  - Živković, Milica
AU  - Miljković, Marge S.
AU  - Ruas-Madiedo, Patricia
AU  - Markelić, Milica B.
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Soković Bajić, Svetlana
AU  - Korac, Aleksandra
AU  - Golić, Nataša
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/989
AB  - The aim of this study was to determine the role of an exopolysaccharide produced by natural dairy isolate Lactobacillus paracasel subsp. paracasel BGSJ2-8, in the adhesion to intestinal epithelial cells and a decrease in Escherichia coil's association with Caco-2 cells. Annotation of the BGSJ2-8 genome showed the presence of a gene cluster, epsSJ, which encodes the biosynthesis of the strain-specific exopolysaccharide EPS-SJ, detected as two fractions (P1 and P2) by size exclusion chromatography (SEC) coupled with multi angle laser light scattering (MALLS) detection. SEC MALLS analysis revealed that an EPS-SJ(-) mutant (EPS7, obtained by insertion mutagenesis of the glps_2198 gene encoding primary glycosyltransferase) does not produce the P2 fraction of EPS-SJ. Transmission electron microscopy showed that EPS7 mutant has a thinner cell wall compared to the EPS-SJ(+) strain BGSJ2-83 (a plasmid free derivative of BGSJ2-8). Interestingly, strain BGSJ2-83 showed higher adhesion to Caco-2 epithelial intestinal cell line than the EPS7 mutant. Accordingly, BGSJ2-83 effectively reduced E. coil ATCC25922's association with Caco-2 cells, while EPS7 did not show statistically significant differences. In addition, the effect of EPS-SJ on the proliferation of lymphocytes in gastrointestinal associated lymphoid tissue (GALT) was tested and the results showed that the reduction of GALT lymphocyte proliferation was higher by BGSJ2-83 than by the mutant. To the best of our knowledge this is the first report indicating that the presence of EPS (EPS-SJ) on the surface of lactobacilli can improve communication between bacteria and intestinal epithelium, implying its possible role in gut colonization.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells
VL  - 7
DO  - 10.3389/fmicb.2016.00286
ER  - 

@article{
author = "Živković, Milica and Miljković, Marge S. and Ruas-Madiedo, Patricia and Markelić, Milica B. and Veljović, Katarina and Tolinački, Maja and Soković Bajić, Svetlana and Korac, Aleksandra and Golić, Nataša",
year = "2016",
abstract = "The aim of this study was to determine the role of an exopolysaccharide produced by natural dairy isolate Lactobacillus paracasel subsp. paracasel BGSJ2-8, in the adhesion to intestinal epithelial cells and a decrease in Escherichia coil's association with Caco-2 cells. Annotation of the BGSJ2-8 genome showed the presence of a gene cluster, epsSJ, which encodes the biosynthesis of the strain-specific exopolysaccharide EPS-SJ, detected as two fractions (P1 and P2) by size exclusion chromatography (SEC) coupled with multi angle laser light scattering (MALLS) detection. SEC MALLS analysis revealed that an EPS-SJ(-) mutant (EPS7, obtained by insertion mutagenesis of the glps_2198 gene encoding primary glycosyltransferase) does not produce the P2 fraction of EPS-SJ. Transmission electron microscopy showed that EPS7 mutant has a thinner cell wall compared to the EPS-SJ(+) strain BGSJ2-83 (a plasmid free derivative of BGSJ2-8). Interestingly, strain BGSJ2-83 showed higher adhesion to Caco-2 epithelial intestinal cell line than the EPS7 mutant. Accordingly, BGSJ2-83 effectively reduced E. coil ATCC25922's association with Caco-2 cells, while EPS7 did not show statistically significant differences. In addition, the effect of EPS-SJ on the proliferation of lymphocytes in gastrointestinal associated lymphoid tissue (GALT) was tested and the results showed that the reduction of GALT lymphocyte proliferation was higher by BGSJ2-83 than by the mutant. To the best of our knowledge this is the first report indicating that the presence of EPS (EPS-SJ) on the surface of lactobacilli can improve communication between bacteria and intestinal epithelium, implying its possible role in gut colonization.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells",
volume = "7",
doi = "10.3389/fmicb.2016.00286"
}

Živković, M., Miljković, M. S., Ruas-Madiedo, P., Markelić, M. B., Veljović, K., Tolinački, M., Soković Bajić, S., Korac, A.,& Golić, N.. (2016). EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 7.
https://doi.org/10.3389/fmicb.2016.00286

Živković M, Miljković MS, Ruas-Madiedo P, Markelić MB, Veljović K, Tolinački M, Soković Bajić S, Korac A, Golić N. EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells. in Frontiers in Microbiology. 2016;7.
doi:10.3389/fmicb.2016.00286 .

Živković, Milica, Miljković, Marge S., Ruas-Madiedo, Patricia, Markelić, Milica B., Veljović, Katarina, Tolinački, Maja, Soković Bajić, Svetlana, Korac, Aleksandra, Golić, Nataša, "EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E-coil Association to Caco-2 Cells" in Frontiers in Microbiology, 7 (2016),
https://doi.org/10.3389/fmicb.2016.00286 . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Begović, Jelena
AU  - Filipić, Brankica
AU  - Popović, Dušanka
AU  - Tolinački, Maja
AU  - Miljković, Marija
AU  - Kojić, Milan
AU  - Golić, Nataša
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/820
AB  - Enterococci represent the most controversial group of dairy bacteria. They are found to be the main constituent of many traditional Mediterranean dairy products and contribute to their characteristic taste and flavor. On the other hand, during the last 50 years antibiotic resistant enterococci have emerged as leading causes of nosocomial infections worldwide. The aim of this study was to determine the diversity, technological properties, antibiotic susceptibility and virulence traits of 636 enterococci previously isolated from 55 artisan dairy products from 12 locations in the Western Balkan countries (WBC) of Serbia, Croatia and Bosnia and Herzegovina. All strains were identified both by microbiological and molecular methods. The predominant species was Enterococcus durans, followed by Enterococcus faecalis and Enterococcus faecium. Over 44% of the isolates were resistant to ciprofloxacin and erythromycin, while 26.2% of the isolates were multi resistant to three or more antibiotics belonging to different families. 185 isolates (29.1%) were susceptible to all 13 of the antibiotics tested. The antibiotic-susceptible isolates were further tested for possible virulence genes and the production of biogenic amines. Finally, five enterococci isolates were found to be antibiotic susceptible with good technological characteristics and without virulence traits or the ability to produce biogenic amines, making them possible candidates for biotechnological application as starter cultures in the dairy industry.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?
VL  - 6
DO  - 10.3389/fmicb.2015.00954
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Begović, Jelena and Filipić, Brankica and Popović, Dušanka and Tolinački, Maja and Miljković, Marija and Kojić, Milan and Golić, Nataša",
year = "2015",
abstract = "Enterococci represent the most controversial group of dairy bacteria. They are found to be the main constituent of many traditional Mediterranean dairy products and contribute to their characteristic taste and flavor. On the other hand, during the last 50 years antibiotic resistant enterococci have emerged as leading causes of nosocomial infections worldwide. The aim of this study was to determine the diversity, technological properties, antibiotic susceptibility and virulence traits of 636 enterococci previously isolated from 55 artisan dairy products from 12 locations in the Western Balkan countries (WBC) of Serbia, Croatia and Bosnia and Herzegovina. All strains were identified both by microbiological and molecular methods. The predominant species was Enterococcus durans, followed by Enterococcus faecalis and Enterococcus faecium. Over 44% of the isolates were resistant to ciprofloxacin and erythromycin, while 26.2% of the isolates were multi resistant to three or more antibiotics belonging to different families. 185 isolates (29.1%) were susceptible to all 13 of the antibiotics tested. The antibiotic-susceptible isolates were further tested for possible virulence genes and the production of biogenic amines. Finally, five enterococci isolates were found to be antibiotic susceptible with good technological characteristics and without virulence traits or the ability to produce biogenic amines, making them possible candidates for biotechnological application as starter cultures in the dairy industry.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?",
volume = "6",
doi = "10.3389/fmicb.2015.00954"
}

Terzić-Vidojević, A., Veljović, K., Begović, J., Filipić, B., Popović, D., Tolinački, M., Miljković, M., Kojić, M.,& Golić, N.. (2015). Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 6.
https://doi.org/10.3389/fmicb.2015.00954

Terzić-Vidojević A, Veljović K, Begović J, Filipić B, Popović D, Tolinački M, Miljković M, Kojić M, Golić N. Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?. in Frontiers in Microbiology. 2015;6.
doi:10.3389/fmicb.2015.00954 .

Terzić-Vidojević, Amarela, Veljović, Katarina, Begović, Jelena, Filipić, Brankica, Popović, Dušanka, Tolinački, Maja, Miljković, Marija, Kojić, Milan, Golić, Nataša, "Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?" in Frontiers in Microbiology, 6 (2015),
https://doi.org/10.3389/fmicb.2015.00954 . .

TY  - JOUR
AU  - Novović, Katarina
AU  - Filipić, B.
AU  - Veljović, Katarina
AU  - Begović, Jelena
AU  - Mirković, N.
AU  - Jovčić, Branko
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/893
AB  - New Delhi metallo-beta-lactamase-1 (NDM-1) will soon become the most commonly isolated and distributed metallo-beta-lactamase worldwide due to its rapid international dissemination and its ability to be expressed by numerous Gram-negative pathogens. NDM-positive bacteria pose a significant public health threat in the Indian subcontinent and the Balkans, which have been designated as endemic regions. Our study was focused on urban rivers, a lake and springheads as a potential source of NDM-1-producing strains in Serbia, but also as a source of other metallo-beta-lactamases and extended-spectrum beta-lactamase (ESBL) producing bacteria. A total of 69 beta-lactam resistant isolates, belonging to 12 bacterial genera, were collected from 8 out of 10 different locations in Belgrade, of which the most were from a popular recreational site, Ada Ciganlija Lake. Phenotypic tests revealed 7 (10.14%) ESBL-producing isolates and 39 (56.52%) isolates resistant to imipenem, of which 32 were positive for metallo-beta-lactamase (MBL) production. PCR and sequencing revealed the presence of genetic determinants for SHV (3 isolates), DHA-1 (1 isolate) and CMY-2 (1 isolate) beta-lactamases. However, we did not detect any NDM-1-producing strains (previously described cases of NDM-1 from Serbia were limited to Belgrade), so we propose that Serbian NDM-1 is in fact a transplant and a nosocomial, rather than an environmental, issue and that Serbia is not an endemic region for NDM-1.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned
EP  - 398
SP  - 393
VL  - 511
DO  - 10.1016/j.scitotenv.2014.12.072
ER  - 

@article{
author = "Novović, Katarina and Filipić, B. and Veljović, Katarina and Begović, Jelena and Mirković, N. and Jovčić, Branko",
year = "2015",
abstract = "New Delhi metallo-beta-lactamase-1 (NDM-1) will soon become the most commonly isolated and distributed metallo-beta-lactamase worldwide due to its rapid international dissemination and its ability to be expressed by numerous Gram-negative pathogens. NDM-positive bacteria pose a significant public health threat in the Indian subcontinent and the Balkans, which have been designated as endemic regions. Our study was focused on urban rivers, a lake and springheads as a potential source of NDM-1-producing strains in Serbia, but also as a source of other metallo-beta-lactamases and extended-spectrum beta-lactamase (ESBL) producing bacteria. A total of 69 beta-lactam resistant isolates, belonging to 12 bacterial genera, were collected from 8 out of 10 different locations in Belgrade, of which the most were from a popular recreational site, Ada Ciganlija Lake. Phenotypic tests revealed 7 (10.14%) ESBL-producing isolates and 39 (56.52%) isolates resistant to imipenem, of which 32 were positive for metallo-beta-lactamase (MBL) production. PCR and sequencing revealed the presence of genetic determinants for SHV (3 isolates), DHA-1 (1 isolate) and CMY-2 (1 isolate) beta-lactamases. However, we did not detect any NDM-1-producing strains (previously described cases of NDM-1 from Serbia were limited to Belgrade), so we propose that Serbian NDM-1 is in fact a transplant and a nosocomial, rather than an environmental, issue and that Serbia is not an endemic region for NDM-1.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned",
pages = "398-393",
volume = "511",
doi = "10.1016/j.scitotenv.2014.12.072"
}

Novović, K., Filipić, B., Veljović, K., Begović, J., Mirković, N.,& Jovčić, B.. (2015). Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned. in Science of the Total Environment
Elsevier, Amsterdam., 511, 393-398.
https://doi.org/10.1016/j.scitotenv.2014.12.072

Novović K, Filipić B, Veljović K, Begović J, Mirković N, Jovčić B. Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned. in Science of the Total Environment. 2015;511:393-398.
doi:10.1016/j.scitotenv.2014.12.072 .

Novović, Katarina, Filipić, B., Veljović, Katarina, Begović, Jelena, Mirković, N., Jovčić, Branko, "Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned" in Science of the Total Environment, 511 (2015):393-398,
https://doi.org/10.1016/j.scitotenv.2014.12.072 . .

TY  - JOUR
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Terzić-Vidojević, Amarela
AU  - Tolinački, Maja
AU  - Mihajlović, Sanja
AU  - Jovčić, Branko
AU  - Kojić, Milan
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/818
AB  - Despite the number of studies on antibiotic-resistant enterococci from Serbian clinical settings, there are no data about environmental contamination with these bacteria. Thus, this study investigated the prevalence of antibiotic-resistant enterococci in Belgrade, Serbia. Enterococcus species collected from ten surface water sites, including a lake, two major river systems, and springs, were tested. Among enterococci, we found single (21.7 %), double (17.4 %), and multiple antibiotic resistance patterns (56.3 %). Vancomycin-resistant strains were not found, indicating that their abundance in Belgrade is tightly linked to clinical settings. The multiple drug-resistant strains Enterococcus faecalis, Enterococcus faecium, and Enterococcus mundtii were frequently detected in the lake during the swimming season and in the rivers near industrial zones. We confirmed the presence of ermB, ermC, ant(6)-Ia, tetM, and tetL and mutations in gyrA genes. The phylogenetic analysis of 16S rRNA gene of E. faecium isolates that harbor esp gene classified them into two groups based on high-bootstraps scores in the tree analysis. Pulsed-field gel electrophoresis analysis of antibiotic-resistant enterococci revealed genomic similarity ranging from 75 to 100 %. This study indicates the importance of anthropogenic impact to the spread of antibiotic-resistant enterococci in environmental waters of Belgrade, Serbia.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia
IS  - 9
VL  - 187
DO  - 10.1007/s10661-015-4814-x
ER  - 

@article{
author = "Veljović, Katarina and Popović, Nikola and Terzić-Vidojević, Amarela and Tolinački, Maja and Mihajlović, Sanja and Jovčić, Branko and Kojić, Milan",
year = "2015",
abstract = "Despite the number of studies on antibiotic-resistant enterococci from Serbian clinical settings, there are no data about environmental contamination with these bacteria. Thus, this study investigated the prevalence of antibiotic-resistant enterococci in Belgrade, Serbia. Enterococcus species collected from ten surface water sites, including a lake, two major river systems, and springs, were tested. Among enterococci, we found single (21.7 %), double (17.4 %), and multiple antibiotic resistance patterns (56.3 %). Vancomycin-resistant strains were not found, indicating that their abundance in Belgrade is tightly linked to clinical settings. The multiple drug-resistant strains Enterococcus faecalis, Enterococcus faecium, and Enterococcus mundtii were frequently detected in the lake during the swimming season and in the rivers near industrial zones. We confirmed the presence of ermB, ermC, ant(6)-Ia, tetM, and tetL and mutations in gyrA genes. The phylogenetic analysis of 16S rRNA gene of E. faecium isolates that harbor esp gene classified them into two groups based on high-bootstraps scores in the tree analysis. Pulsed-field gel electrophoresis analysis of antibiotic-resistant enterococci revealed genomic similarity ranging from 75 to 100 %. This study indicates the importance of anthropogenic impact to the spread of antibiotic-resistant enterococci in environmental waters of Belgrade, Serbia.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia",
number = "9",
volume = "187",
doi = "10.1007/s10661-015-4814-x"
}

Veljović, K., Popović, N., Terzić-Vidojević, A., Tolinački, M., Mihajlović, S., Jovčić, B.,& Kojić, M.. (2015). Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia. in Environmental Monitoring and Assessment
Springer, Dordrecht., 187(9).
https://doi.org/10.1007/s10661-015-4814-x

Veljović K, Popović N, Terzić-Vidojević A, Tolinački M, Mihajlović S, Jovčić B, Kojić M. Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia. in Environmental Monitoring and Assessment. 2015;187(9).
doi:10.1007/s10661-015-4814-x .

Veljović, Katarina, Popović, Nikola, Terzić-Vidojević, Amarela, Tolinački, Maja, Mihajlović, Sanja, Jovčić, Branko, Kojić, Milan, "Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia" in Environmental Monitoring and Assessment, 187, no. 9 (2015),
https://doi.org/10.1007/s10661-015-4814-x . .