TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Soković Bajić, Svetlana
AU  - Jovanović, Živko
AU  - Matić, Ivana
AU  - Tolinački, Maja
AU  - Popović, Dušanka
AU  - Popović, Nikola
AU  - Terzić-Vidojević, Amarela
AU  - Golić, Nataša
AU  - Beskoski, Vladimir
AU  - Samardžić, Jelena
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1337
AB  - In this study, for the first time, the comprehensive analysis of antiproliferative and antioxidant activities of ramson, followed by the analysis of its associated microbiota and health-promoting effects of lactic acid bacteria (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant with a long history of use in traditional medicine due to its antimicrobial and antioxidant activity. In this study the influence of in vitro gastrointestinal digestion on the cytotoxic activity of A. ursinum extracts against human malignant cell lines was demonstrated. Seven sulfur compounds, the degradation products of thiosulfinates, including diallyl disulfide were shown to inhibit proliferation of malignant cells by inducing accumulation within G2/M phase as well as to induce apoptosis through activation of caspase-3 and mitochondrial signaling pathway. Further, the A. ursinum microbiota, particularly LAB with potential probiotic effects, was analyzed by culture-dependent method and culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The obtained results revealed that the most abundant genera were Streptococcus, Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the presence of two PFGE pulsotypes. The probiotic potential of the strain L. fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed that both strains are safe for human use, successfully survive the simulated gastrointestinal conditions, have potential to transiently colonize the gastrointestinal tract (GIT) and have a protective immunomodulatory effect, inducing the production of proinflammatory cytokine IL17 and regulatory cytokine IL10, while decreasing the production of proinflammatory cytokine IFN-gamma. In conclusion, the results of this study suggest that consumption of A. ursinum might have health-promoting properties, including anticancer effects, while L. fermentum strains isolated from A. ursinum leaves could be used as probiotics for human consumption.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix
VL  - 11
DO  - 10.3389/fmicb.2020.601616
ER  - 

@article{
author = "Stanisavljević, Nemanja and Soković Bajić, Svetlana and Jovanović, Živko and Matić, Ivana and Tolinački, Maja and Popović, Dušanka and Popović, Nikola and Terzić-Vidojević, Amarela and Golić, Nataša and Beskoski, Vladimir and Samardžić, Jelena",
year = "2020",
abstract = "In this study, for the first time, the comprehensive analysis of antiproliferative and antioxidant activities of ramson, followed by the analysis of its associated microbiota and health-promoting effects of lactic acid bacteria (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant with a long history of use in traditional medicine due to its antimicrobial and antioxidant activity. In this study the influence of in vitro gastrointestinal digestion on the cytotoxic activity of A. ursinum extracts against human malignant cell lines was demonstrated. Seven sulfur compounds, the degradation products of thiosulfinates, including diallyl disulfide were shown to inhibit proliferation of malignant cells by inducing accumulation within G2/M phase as well as to induce apoptosis through activation of caspase-3 and mitochondrial signaling pathway. Further, the A. ursinum microbiota, particularly LAB with potential probiotic effects, was analyzed by culture-dependent method and culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The obtained results revealed that the most abundant genera were Streptococcus, Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the presence of two PFGE pulsotypes. The probiotic potential of the strain L. fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed that both strains are safe for human use, successfully survive the simulated gastrointestinal conditions, have potential to transiently colonize the gastrointestinal tract (GIT) and have a protective immunomodulatory effect, inducing the production of proinflammatory cytokine IL17 and regulatory cytokine IL10, while decreasing the production of proinflammatory cytokine IFN-gamma. In conclusion, the results of this study suggest that consumption of A. ursinum might have health-promoting properties, including anticancer effects, while L. fermentum strains isolated from A. ursinum leaves could be used as probiotics for human consumption.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix",
volume = "11",
doi = "10.3389/fmicb.2020.601616"
}

Stanisavljević, N., Soković Bajić, S., Jovanović, Ž., Matić, I., Tolinački, M., Popović, D., Popović, N., Terzić-Vidojević, A., Golić, N., Beskoski, V.,& Samardžić, J.. (2020). Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 11.
https://doi.org/10.3389/fmicb.2020.601616

Stanisavljević N, Soković Bajić S, Jovanović Ž, Matić I, Tolinački M, Popović D, Popović N, Terzić-Vidojević A, Golić N, Beskoski V, Samardžić J. Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix. in Frontiers in Microbiology. 2020;11.
doi:10.3389/fmicb.2020.601616 .

Stanisavljević, Nemanja, Soković Bajić, Svetlana, Jovanović, Živko, Matić, Ivana, Tolinački, Maja, Popović, Dušanka, Popović, Nikola, Terzić-Vidojević, Amarela, Golić, Nataša, Beskoski, Vladimir, Samardžić, Jelena, "Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix" in Frontiers in Microbiology, 11 (2020),
https://doi.org/10.3389/fmicb.2020.601616 . .

TY  - JOUR
AU  - Selaković, Sara D.
AU  - Stanisavljević, Nemanja
AU  - Vujić, Vukica D.
AU  - Rubinjoni, Luka Z.
AU  - Jovanović, Živko
AU  - Radović, Svetlana R.
AU  - Cvetković, Dragana D.
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1096
AB  - Interactions between plants and herbivorous insects can be shaped by light environment, resulting in differential herbivory in sun and shade. In dioecious species, the combination of plant sex and light-induced changes in defense traits and nutritive value can alter the patterns of foliar damage. We explored the combined effects of light environment and plant sex on natural herbivore damage and plant traits in the dioecious understory forb Mercurialis perennis on Mt. Kopaonik (Serbia). The role of plant traits in predicting the extent of damage was examined as well. Male and female plants from contrasting light environments (shade vs. sun) were analyzed with respect to leaf damage, as well as plant morphological and biochemical traits (size, specific leaf area, carbon-based defensive compounds and nutritional quality). We found prominent differences in herbivore damage between sun and shade conditions, but not between the sexes. Plants from the sun-exposed site had a significantly larger leaf area removed. The specific leaf area co-varied with herbivore damage in an inverse fashion, while leaf nutritional value had a moderate effect. Contrasting light conditions influenced the patterns of intersexual differences in the contents of condensed tannins and soluble proteins, with females exhibiting higher trait values. We found that factors defining risk of consumption were related to plant morphological traits and nutritive value rather than to chemical defenses.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Light and sex interplay: differential herbivore damage in sun and shade in dioecious Mercurialis perennis
EP  - 479
IS  - 3
SP  - 469
VL  - 70
DO  - 10.2298/ABS171207007S
ER  - 

@article{
author = "Selaković, Sara D. and Stanisavljević, Nemanja and Vujić, Vukica D. and Rubinjoni, Luka Z. and Jovanović, Živko and Radović, Svetlana R. and Cvetković, Dragana D.",
year = "2018",
abstract = "Interactions between plants and herbivorous insects can be shaped by light environment, resulting in differential herbivory in sun and shade. In dioecious species, the combination of plant sex and light-induced changes in defense traits and nutritive value can alter the patterns of foliar damage. We explored the combined effects of light environment and plant sex on natural herbivore damage and plant traits in the dioecious understory forb Mercurialis perennis on Mt. Kopaonik (Serbia). The role of plant traits in predicting the extent of damage was examined as well. Male and female plants from contrasting light environments (shade vs. sun) were analyzed with respect to leaf damage, as well as plant morphological and biochemical traits (size, specific leaf area, carbon-based defensive compounds and nutritional quality). We found prominent differences in herbivore damage between sun and shade conditions, but not between the sexes. Plants from the sun-exposed site had a significantly larger leaf area removed. The specific leaf area co-varied with herbivore damage in an inverse fashion, while leaf nutritional value had a moderate effect. Contrasting light conditions influenced the patterns of intersexual differences in the contents of condensed tannins and soluble proteins, with females exhibiting higher trait values. We found that factors defining risk of consumption were related to plant morphological traits and nutritive value rather than to chemical defenses.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Light and sex interplay: differential herbivore damage in sun and shade in dioecious Mercurialis perennis",
pages = "479-469",
number = "3",
volume = "70",
doi = "10.2298/ABS171207007S"
}

Selaković, S. D., Stanisavljević, N., Vujić, V. D., Rubinjoni, L. Z., Jovanović, Ž., Radović, S. R.,& Cvetković, D. D.. (2018). Light and sex interplay: differential herbivore damage in sun and shade in dioecious Mercurialis perennis. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 70(3), 469-479.
https://doi.org/10.2298/ABS171207007S

Selaković SD, Stanisavljević N, Vujić VD, Rubinjoni LZ, Jovanović Ž, Radović SR, Cvetković DD. Light and sex interplay: differential herbivore damage in sun and shade in dioecious Mercurialis perennis. in Archives of Biological Sciences. 2018;70(3):469-479.
doi:10.2298/ABS171207007S .

Selaković, Sara D., Stanisavljević, Nemanja, Vujić, Vukica D., Rubinjoni, Luka Z., Jovanović, Živko, Radović, Svetlana R., Cvetković, Dragana D., "Light and sex interplay: differential herbivore damage in sun and shade in dioecious Mercurialis perennis" in Archives of Biological Sciences, 70, no. 3 (2018):469-479,
https://doi.org/10.2298/ABS171207007S . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Savić, Jelena
AU  - Jovanović, Živko
AU  - Miljuš-Đukić, Jovanka
AU  - Sencanski, Jelena
AU  - Simonović, Mladen
AU  - Radović, Svetlana
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1181
AB  - This study investigated the role of antioxidant system of Alyssum markgrafii, during long-term exposure to 0.5 or 1 mM NiCl2 x 6H(2)O in vitro. Applied methodology included sample preparation protocol which reduces oxidation of key metabolites along with novel luminescent method and well-established photometric procedures. During 5-week treatments, plants accumulated 1121 and 2470 ppm of Ni2+ respectively, followed by severe growth retardation, chlorophyll degradation and peroxidation of lipids. These effects were more pronounced after 1 mM Ni2+ treatment and additionally accompanied by increased water loss. Activities of luminol-converting peroxidases and glutathione reductase upon 0.5 mM treatment were increased while catalase and superoxide dismutase were diminished. The fact that these two groups of enzymes run in antiparallel might suggest functional redistribution between antioxidant enzymes rather than orchestrated action to prevent oxidative damage. Total antioxidant capacity (TAC) was also increased after 0.5 mM treatment which coincided with increased GR activity and elevated glutathione content indicating this low molecular weight antioxidant as an important factor associated with nickel tolerance. This study also emphasizes the possible important role of luminol-converting peroxidases in nickel hyperaccumulation, although they are not considered as antioxidant enzymes sensu stricto since some of them can also produce reactive oxygen species as well.
PB  - Springer Heidelberg, Heidelberg
T2  - Acta Physiologiae Plantarum
T1  - Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro
IS  - 6
VL  - 40
DO  - 10.1007/s11738-018-2677-9
ER  - 

@article{
author = "Stanisavljević, Nemanja and Savić, Jelena and Jovanović, Živko and Miljuš-Đukić, Jovanka and Sencanski, Jelena and Simonović, Mladen and Radović, Svetlana and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2018",
abstract = "This study investigated the role of antioxidant system of Alyssum markgrafii, during long-term exposure to 0.5 or 1 mM NiCl2 x 6H(2)O in vitro. Applied methodology included sample preparation protocol which reduces oxidation of key metabolites along with novel luminescent method and well-established photometric procedures. During 5-week treatments, plants accumulated 1121 and 2470 ppm of Ni2+ respectively, followed by severe growth retardation, chlorophyll degradation and peroxidation of lipids. These effects were more pronounced after 1 mM Ni2+ treatment and additionally accompanied by increased water loss. Activities of luminol-converting peroxidases and glutathione reductase upon 0.5 mM treatment were increased while catalase and superoxide dismutase were diminished. The fact that these two groups of enzymes run in antiparallel might suggest functional redistribution between antioxidant enzymes rather than orchestrated action to prevent oxidative damage. Total antioxidant capacity (TAC) was also increased after 0.5 mM treatment which coincided with increased GR activity and elevated glutathione content indicating this low molecular weight antioxidant as an important factor associated with nickel tolerance. This study also emphasizes the possible important role of luminol-converting peroxidases in nickel hyperaccumulation, although they are not considered as antioxidant enzymes sensu stricto since some of them can also produce reactive oxygen species as well.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Acta Physiologiae Plantarum",
title = "Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro",
number = "6",
volume = "40",
doi = "10.1007/s11738-018-2677-9"
}

Stanisavljević, N., Savić, J., Jovanović, Ž., Miljuš-Đukić, J., Sencanski, J., Simonović, M., Radović, S., Vinterhalter, D.,& Vinterhalter, B.. (2018). Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro. in Acta Physiologiae Plantarum
Springer Heidelberg, Heidelberg., 40(6).
https://doi.org/10.1007/s11738-018-2677-9

Stanisavljević N, Savić J, Jovanović Ž, Miljuš-Đukić J, Sencanski J, Simonović M, Radović S, Vinterhalter D, Vinterhalter B. Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro. in Acta Physiologiae Plantarum. 2018;40(6).
doi:10.1007/s11738-018-2677-9 .

Stanisavljević, Nemanja, Savić, Jelena, Jovanović, Živko, Miljuš-Đukić, Jovanka, Sencanski, Jelena, Simonović, Mladen, Radović, Svetlana, Vinterhalter, Dragan, Vinterhalter, Branka, "Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro" in Acta Physiologiae Plantarum, 40, no. 6 (2018),
https://doi.org/10.1007/s11738-018-2677-9 . .

TY  - JOUR
AU  - Selaković, Sara
AU  - Vujić, Vukica
AU  - Stanisavljević, Nemanja
AU  - Jovanović, Živko
AU  - Radović, Svetlana
AU  - Cvetković, Dragana
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1022
AB  - Plant-herbivore interactions can be mediated by plant apparency, defensive and nutritional quality traits that change through plant ontogeny, resulting in age-specific herbivory. In dioecious species, opposing allocation patterns in defense may lead to sex-biased herbivory. Here, we examine how onto stage and plant sex determine levels of herbivore damage in understory herb Mercurialis perennis under field conditions. We analyzed variation in plant size (height, total leaf area), physical (specific leaf area) and chemical (total phenolic and condensed tannins contents) defense, and nutritional quality (total water, soluble protein and nonstructural carbohydrate contents) during the shift from reproductive to post-reproductive stage. Furthermore, we explored correlations between the analyzed traits and levels of foliar damage. Post-reproductive plants had lower levels of chemical defense, and larger leaf area removed, in spite of having lower nutritive quality. Opposing patterns of intersexual differences were detected in protein and phenolic contents during reproductive stage, while in post-reproductive stage total leaf area was sexually dimorphic. Female-biased herbivory was apparent only after reproduction. Plant size parameters combined with condensed tannins content determined levels of foliar damage during post reproductive stage, while the only trait covarying with herbivory in reproductive stage was total nonstructural carbohydrate content. Our results support claims of optimal defense theory - sensitive stage of reproduction was better defended. We conclude that different combinations of plant traits mediated interactions with herbivores in mature stages. Differences in reproductive allocation between the sexes may not immediately translate into different levels of damage, stressing the need for considering different ontogenetic stages when exploring sex bias in herbivory.
PB  - Elsevier, Amsterdam
T2  - Acta Oecologica-International Journal of Ecology
T1  - Ontogenetic stage, plant vigor and sex mediate herbivory loads in a dioecious understory herb
EP  - 190
SP  - 184
VL  - 85
DO  - 10.1016/j.actao.2017.11.006
ER  - 

@article{
author = "Selaković, Sara and Vujić, Vukica and Stanisavljević, Nemanja and Jovanović, Živko and Radović, Svetlana and Cvetković, Dragana",
year = "2017",
abstract = "Plant-herbivore interactions can be mediated by plant apparency, defensive and nutritional quality traits that change through plant ontogeny, resulting in age-specific herbivory. In dioecious species, opposing allocation patterns in defense may lead to sex-biased herbivory. Here, we examine how onto stage and plant sex determine levels of herbivore damage in understory herb Mercurialis perennis under field conditions. We analyzed variation in plant size (height, total leaf area), physical (specific leaf area) and chemical (total phenolic and condensed tannins contents) defense, and nutritional quality (total water, soluble protein and nonstructural carbohydrate contents) during the shift from reproductive to post-reproductive stage. Furthermore, we explored correlations between the analyzed traits and levels of foliar damage. Post-reproductive plants had lower levels of chemical defense, and larger leaf area removed, in spite of having lower nutritive quality. Opposing patterns of intersexual differences were detected in protein and phenolic contents during reproductive stage, while in post-reproductive stage total leaf area was sexually dimorphic. Female-biased herbivory was apparent only after reproduction. Plant size parameters combined with condensed tannins content determined levels of foliar damage during post reproductive stage, while the only trait covarying with herbivory in reproductive stage was total nonstructural carbohydrate content. Our results support claims of optimal defense theory - sensitive stage of reproduction was better defended. We conclude that different combinations of plant traits mediated interactions with herbivores in mature stages. Differences in reproductive allocation between the sexes may not immediately translate into different levels of damage, stressing the need for considering different ontogenetic stages when exploring sex bias in herbivory.",
publisher = "Elsevier, Amsterdam",
journal = "Acta Oecologica-International Journal of Ecology",
title = "Ontogenetic stage, plant vigor and sex mediate herbivory loads in a dioecious understory herb",
pages = "190-184",
volume = "85",
doi = "10.1016/j.actao.2017.11.006"
}

Selaković, S., Vujić, V., Stanisavljević, N., Jovanović, Ž., Radović, S.,& Cvetković, D.. (2017). Ontogenetic stage, plant vigor and sex mediate herbivory loads in a dioecious understory herb. in Acta Oecologica-International Journal of Ecology
Elsevier, Amsterdam., 85, 184-190.
https://doi.org/10.1016/j.actao.2017.11.006

Selaković S, Vujić V, Stanisavljević N, Jovanović Ž, Radović S, Cvetković D. Ontogenetic stage, plant vigor and sex mediate herbivory loads in a dioecious understory herb. in Acta Oecologica-International Journal of Ecology. 2017;85:184-190.
doi:10.1016/j.actao.2017.11.006 .

Selaković, Sara, Vujić, Vukica, Stanisavljević, Nemanja, Jovanović, Živko, Radović, Svetlana, Cvetković, Dragana, "Ontogenetic stage, plant vigor and sex mediate herbivory loads in a dioecious understory herb" in Acta Oecologica-International Journal of Ecology, 85 (2017):184-190,
https://doi.org/10.1016/j.actao.2017.11.006 . .

TY  - JOUR
AU  - Mihailović, Nevena
AU  - Marković, Violeta
AU  - Matić, Ivana Z.
AU  - Stanisavljević, Nemanja
AU  - Jovanović, Živko
AU  - Trifunović, Snežana
AU  - Joksović, Ljubinka
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1058
AB  - Eight 1,3,4-oxadiazole derivatives containing phenolic acid moieties (7a-h) and eight of their diacylhydrazine precursors (6a-h) were synthesized, characterized using spectroscopic methods and examined by scavenging of stable DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals. The most potent phenolic 1,3,4-oxadiazoles showed better DPPH scavenging activity in comparison with their corresponding diacylhydrazine precursors as a result of participation of both aromatic rings and a 1,3,4-oxadiazole moiety in resonance stabilization of the formed phenoxyl radical. Four diacylhydrazines (6d, 6e, 6g, and 6h) and four 1,3,4-oxadiazoles (7d, 7e, 7g and 7h) with the best DPPH scavenging activity, were chosen for further evaluation of their antioxidant potential through various assays. The investigated compounds exerted pronounced ABTS radical scavenging capacity, moderate to good H2O2 scavenging properties and strong ferric ion reducing capacity. Further in vitro evaluation of the antioxidant properties of the most active compounds demonstrated their protective effects in normal lung fibroblasts MRC-5 against hydrogen peroxide induced oxidative stress. Diacylhydrazine 6h increased two times the activity of glutathione peroxidase in treated cells in comparison with a control sample and did not affect the superoxide dismutase activity.
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids
EP  - 8560
IS  - 14
SP  - 8550
VL  - 7
DO  - 10.1039/c6ra28787e
ER  - 

@article{
author = "Mihailović, Nevena and Marković, Violeta and Matić, Ivana Z. and Stanisavljević, Nemanja and Jovanović, Živko and Trifunović, Snežana and Joksović, Ljubinka",
year = "2017",
abstract = "Eight 1,3,4-oxadiazole derivatives containing phenolic acid moieties (7a-h) and eight of their diacylhydrazine precursors (6a-h) were synthesized, characterized using spectroscopic methods and examined by scavenging of stable DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals. The most potent phenolic 1,3,4-oxadiazoles showed better DPPH scavenging activity in comparison with their corresponding diacylhydrazine precursors as a result of participation of both aromatic rings and a 1,3,4-oxadiazole moiety in resonance stabilization of the formed phenoxyl radical. Four diacylhydrazines (6d, 6e, 6g, and 6h) and four 1,3,4-oxadiazoles (7d, 7e, 7g and 7h) with the best DPPH scavenging activity, were chosen for further evaluation of their antioxidant potential through various assays. The investigated compounds exerted pronounced ABTS radical scavenging capacity, moderate to good H2O2 scavenging properties and strong ferric ion reducing capacity. Further in vitro evaluation of the antioxidant properties of the most active compounds demonstrated their protective effects in normal lung fibroblasts MRC-5 against hydrogen peroxide induced oxidative stress. Diacylhydrazine 6h increased two times the activity of glutathione peroxidase in treated cells in comparison with a control sample and did not affect the superoxide dismutase activity.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids",
pages = "8560-8550",
number = "14",
volume = "7",
doi = "10.1039/c6ra28787e"
}

Mihailović, N., Marković, V., Matić, I. Z., Stanisavljević, N., Jovanović, Ž., Trifunović, S.,& Joksović, L.. (2017). Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids. in RSC Advances
Royal Soc Chemistry, Cambridge., 7(14), 8550-8560.
https://doi.org/10.1039/c6ra28787e

Mihailović N, Marković V, Matić IZ, Stanisavljević N, Jovanović Ž, Trifunović S, Joksović L. Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids. in RSC Advances. 2017;7(14):8550-8560.
doi:10.1039/c6ra28787e .

Mihailović, Nevena, Marković, Violeta, Matić, Ivana Z., Stanisavljević, Nemanja, Jovanović, Živko, Trifunović, Snežana, Joksović, Ljubinka, "Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids" in RSC Advances, 7, no. 14 (2017):8550-8560,
https://doi.org/10.1039/c6ra28787e . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Ilić, Marija D.
AU  - Matić, Ivana Z.
AU  - Jovanović, Živko
AU  - Cupić, Tihomir
AU  - Dabić, Dragana C.
AU  - Natić, Maja M.
AU  - Tesić, Zivoslav Lj.
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/905
AB  - To date little has been done on identification of major phenolic compounds responsible for anticancer and antioxidant properties of pea (Pisum sativum L.) seed coat extracts. In the present study, phenolic profile of the seed coat extracts from 10 differently colored European varieties has been determined using ultrahigh-performance liquid chromatography-linear trap quadrupole orbitrap mass spectrometer technique. Extracts of dark colored varieties with high total phenolic content (up to 46.56mg GAE/g) exhibited strong antioxidant activities (measured by 2,2-diphenyl-1-picrylhydrazyl or DPPH assay, and ferric ion reducing and ferrous ion chelating capacity assays) which could be attributed to presence of gallic acid, epigallocatechin, naringenin, and apigenin. The aqueous extracts of dark colored varieties exert concentration-dependent cytotoxic effects on all tested malignant cell lines (human colon adenocarcinoma LS174, human breast carcinoma MDA-MB-453, human lung carcinoma A594, and myelogenous leukemia K562). Correlation analysis revealed that intensities of cytotoxic activity of the extracts strongly correlated with contents of epigallocatechin and luteolin. Cell cycle analysis on LS174 cells in the presence of caspase-3 inhibitor points out that extracts may activate other cell death modalities besides caspase-3-dependent apoptosis. The study provides evidence that seed coat extracts of dark colored pea varieties might be used as potential cancer-chemopreventive and complementary agents in cancer therapy.
PB  - Routledge Journals, Taylor & Francis Ltd, Abingdon
T2  - Nutrition and Cancer-An International Journal
T1  - Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities
EP  - 1000
IS  - 6
SP  - 988
VL  - 68
DO  - 10.1080/01635581.2016.1190019
ER  - 

@article{
author = "Stanisavljević, Nemanja and Ilić, Marija D. and Matić, Ivana Z. and Jovanović, Živko and Cupić, Tihomir and Dabić, Dragana C. and Natić, Maja M. and Tesić, Zivoslav Lj.",
year = "2016",
abstract = "To date little has been done on identification of major phenolic compounds responsible for anticancer and antioxidant properties of pea (Pisum sativum L.) seed coat extracts. In the present study, phenolic profile of the seed coat extracts from 10 differently colored European varieties has been determined using ultrahigh-performance liquid chromatography-linear trap quadrupole orbitrap mass spectrometer technique. Extracts of dark colored varieties with high total phenolic content (up to 46.56mg GAE/g) exhibited strong antioxidant activities (measured by 2,2-diphenyl-1-picrylhydrazyl or DPPH assay, and ferric ion reducing and ferrous ion chelating capacity assays) which could be attributed to presence of gallic acid, epigallocatechin, naringenin, and apigenin. The aqueous extracts of dark colored varieties exert concentration-dependent cytotoxic effects on all tested malignant cell lines (human colon adenocarcinoma LS174, human breast carcinoma MDA-MB-453, human lung carcinoma A594, and myelogenous leukemia K562). Correlation analysis revealed that intensities of cytotoxic activity of the extracts strongly correlated with contents of epigallocatechin and luteolin. Cell cycle analysis on LS174 cells in the presence of caspase-3 inhibitor points out that extracts may activate other cell death modalities besides caspase-3-dependent apoptosis. The study provides evidence that seed coat extracts of dark colored pea varieties might be used as potential cancer-chemopreventive and complementary agents in cancer therapy.",
publisher = "Routledge Journals, Taylor & Francis Ltd, Abingdon",
journal = "Nutrition and Cancer-An International Journal",
title = "Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities",
pages = "1000-988",
number = "6",
volume = "68",
doi = "10.1080/01635581.2016.1190019"
}

Stanisavljević, N., Ilić, M. D., Matić, I. Z., Jovanović, Ž., Cupić, T., Dabić, D. C., Natić, M. M.,& Tesić, Z. Lj.. (2016). Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities. in Nutrition and Cancer-An International Journal
Routledge Journals, Taylor & Francis Ltd, Abingdon., 68(6), 988-1000.
https://doi.org/10.1080/01635581.2016.1190019

Stanisavljević N, Ilić MD, Matić IZ, Jovanović Ž, Cupić T, Dabić DC, Natić MM, Tesić ZL. Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities. in Nutrition and Cancer-An International Journal. 2016;68(6):988-1000.
doi:10.1080/01635581.2016.1190019 .

Stanisavljević, Nemanja, Ilić, Marija D., Matić, Ivana Z., Jovanović, Živko, Cupić, Tihomir, Dabić, Dragana C., Natić, Maja M., Tesić, Zivoslav Lj., "Identification of Phenolic Compounds from Seed Coats of Differently Colored European Varieties of Pea (Pisum sativum L.) and Characterization of Their Antioxidant and In Vitro Anticancer Activities" in Nutrition and Cancer-An International Journal, 68, no. 6 (2016):988-1000,
https://doi.org/10.1080/01635581.2016.1190019 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran 
AU  - Pastor, Ferenc T.
AU  - Suznjević, Desanka
AU  - Jovanović, Živko
AU  - Strahinić, Ivana
AU  - Fira, Đorđe
AU  - Radović, Svetlana S.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/845
AB  - Nine Lactobacillus strains known for surface proteinase activity were chosen from our collection and tested for their ability to grow in pea seed protein-based medium, and to hydrolyze purified pea proteins in order to produce peptides with antioxidant (AO) activity. Two strains, Lactobacillus rhamnosus BGT10 and Lactobacillus zeae LMG17315, exhibited strong proteolytic activity against pea proteins. The AO activity of the pea hydrolysate fraction, MW  lt  10 kDa, obtained by the fermentation of purified pea proteins with Lactobacillus rhamnosus BGT10, was tested by standard spectrophotometric assays (DPPH, ABTS, Fe3+-reducing capacity) and the recently developed direct current (DC) polarographic assay. The low molecular weight fraction of the obtained hydrolysate was separated using ion exchange chromatography, while the AO activity of eluted fractions was determined by means of a sensitive DC polarographic assay without previous concentration of samples. Results revealed that the fraction present in low abundance that contained basic peptides possessed the highest antioxidant activity. Based on the obtained results, it can be concluded that Lactobacillus rhamnosus BGT10 should be further investigated as a candidate strain for large-scale production of bioactive peptides from legume proteins.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria
EP  - 1042
IS  - 3
SP  - 1033
VL  - 67
DO  - 10.2298/ABS150130066S
ER  - 

@article{
author = "Stanisavljević, Nemanja and Vukotić, Goran  and Pastor, Ferenc T. and Suznjević, Desanka and Jovanović, Živko and Strahinić, Ivana and Fira, Đorđe and Radović, Svetlana S.",
year = "2015",
abstract = "Nine Lactobacillus strains known for surface proteinase activity were chosen from our collection and tested for their ability to grow in pea seed protein-based medium, and to hydrolyze purified pea proteins in order to produce peptides with antioxidant (AO) activity. Two strains, Lactobacillus rhamnosus BGT10 and Lactobacillus zeae LMG17315, exhibited strong proteolytic activity against pea proteins. The AO activity of the pea hydrolysate fraction, MW  lt  10 kDa, obtained by the fermentation of purified pea proteins with Lactobacillus rhamnosus BGT10, was tested by standard spectrophotometric assays (DPPH, ABTS, Fe3+-reducing capacity) and the recently developed direct current (DC) polarographic assay. The low molecular weight fraction of the obtained hydrolysate was separated using ion exchange chromatography, while the AO activity of eluted fractions was determined by means of a sensitive DC polarographic assay without previous concentration of samples. Results revealed that the fraction present in low abundance that contained basic peptides possessed the highest antioxidant activity. Based on the obtained results, it can be concluded that Lactobacillus rhamnosus BGT10 should be further investigated as a candidate strain for large-scale production of bioactive peptides from legume proteins.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria",
pages = "1042-1033",
number = "3",
volume = "67",
doi = "10.2298/ABS150130066S"
}

Stanisavljević, N., Vukotić, G., Pastor, F. T., Suznjević, D., Jovanović, Ž., Strahinić, I., Fira, Đ.,& Radović, S. S.. (2015). Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 67(3), 1033-1042.
https://doi.org/10.2298/ABS150130066S

Stanisavljević N, Vukotić G, Pastor FT, Suznjević D, Jovanović Ž, Strahinić I, Fira Đ, Radović SS. Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria. in Archives of Biological Sciences. 2015;67(3):1033-1042.
doi:10.2298/ABS150130066S .

Stanisavljević, Nemanja, Vukotić, Goran , Pastor, Ferenc T., Suznjević, Desanka, Jovanović, Živko, Strahinić, Ivana, Fira, Đorđe, Radović, Svetlana S., "Antioxidant activity of pea protein hydrolysates produced by batch fermentation with lactic acid bacteria" in Archives of Biological Sciences, 67, no. 3 (2015):1033-1042,
https://doi.org/10.2298/ABS150130066S . .

TY  - JOUR
AU  - Aleksić, Jelena M.
AU  - Banović Đeri, Bojana
AU  - Miljuš-Đukić, Jovanka
AU  - Jovanović, Živko
AU  - Mikić, Aleksandar
AU  - Cupina, Branko
AU  - Zlatković, Bojan
AU  - Anđelković, Snežana
AU  - Spanu, Ilaria
AU  - Jelić, Mihailo
AU  - Maksimović, Vesna R.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/834
AB  - Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important food and feed grain crop legume, they have not yet been used for comprehensive molecular characterization of this crop. We report a three-step procedure for rapid and cost-effective delineation and utilization of informative genomic nuclear SSRs for paralleled genotyping in faba bean suitable also for other species: (i) pre-selection of loci generating PCR products of expected lengths which are potentially polymorphic (achieved by PCR amplification in bulked samples); (ii) exclusion of loci burdened with persistent null alleles and multilocus amplification products (based on PCR amplification of pre-selected loci in individual genotypes), and (iii) multiplexing. We demonstrate also that genomic SSRs are promising molecular tools for molecular characterization of faba bean required also for crop improvement.
PB  - Czech Academy Agricultural Sciences, Prague
T2  - Czech Journal of Genetics and Plant Breeding
T1  - A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba
EP  - 39
IS  - 1
SP  - 36
VL  - 51
DO  - 10.17221/153/2014-CJGPB
ER  - 

@article{
author = "Aleksić, Jelena M. and Banović Đeri, Bojana and Miljuš-Đukić, Jovanka and Jovanović, Živko and Mikić, Aleksandar and Cupina, Branko and Zlatković, Bojan and Anđelković, Snežana and Spanu, Ilaria and Jelić, Mihailo and Maksimović, Vesna R.",
year = "2015",
abstract = "Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important food and feed grain crop legume, they have not yet been used for comprehensive molecular characterization of this crop. We report a three-step procedure for rapid and cost-effective delineation and utilization of informative genomic nuclear SSRs for paralleled genotyping in faba bean suitable also for other species: (i) pre-selection of loci generating PCR products of expected lengths which are potentially polymorphic (achieved by PCR amplification in bulked samples); (ii) exclusion of loci burdened with persistent null alleles and multilocus amplification products (based on PCR amplification of pre-selected loci in individual genotypes), and (iii) multiplexing. We demonstrate also that genomic SSRs are promising molecular tools for molecular characterization of faba bean required also for crop improvement.",
publisher = "Czech Academy Agricultural Sciences, Prague",
journal = "Czech Journal of Genetics and Plant Breeding",
title = "A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba",
pages = "39-36",
number = "1",
volume = "51",
doi = "10.17221/153/2014-CJGPB"
}

Aleksić, J. M., Banović Đeri, B., Miljuš-Đukić, J., Jovanović, Ž., Mikić, A., Cupina, B., Zlatković, B., Anđelković, S., Spanu, I., Jelić, M.,& Maksimović, V. R.. (2015). A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba. in Czech Journal of Genetics and Plant Breeding
Czech Academy Agricultural Sciences, Prague., 51(1), 36-39.
https://doi.org/10.17221/153/2014-CJGPB

Aleksić JM, Banović Đeri B, Miljuš-Đukić J, Jovanović Ž, Mikić A, Cupina B, Zlatković B, Anđelković S, Spanu I, Jelić M, Maksimović VR. A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba. in Czech Journal of Genetics and Plant Breeding. 2015;51(1):36-39.
doi:10.17221/153/2014-CJGPB .

Aleksić, Jelena M., Banović Đeri, Bojana, Miljuš-Đukić, Jovanka, Jovanović, Živko, Mikić, Aleksandar, Cupina, Branko, Zlatković, Bojan, Anđelković, Snežana, Spanu, Ilaria, Jelić, Mihailo, Maksimović, Vesna R., "A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba" in Czech Journal of Genetics and Plant Breeding, 51, no. 1 (2015):36-39,
https://doi.org/10.17221/153/2014-CJGPB . .

TY  - JOUR
AU  - Mikić, Aleksandar
AU  - Medović, Aleksandar
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/812
AB  - Bitter vetch (Vicia ervilia (L.) Willd.) was a part of the everyday diet of the Eurasian Neanderthal population and the modern human Palaeolithic hunter-gatherers at the end of the last Ice Age. The major criteria to determine the domestication in bitter vetch and other ancient grain legumes are non-dehiscent pods, larger seed size and smooth seed testa. Bitter vetch seeds were found among the earliest findings of cultivated crops at the site of Tell El-Kerkh, Syria, from 10th millennium BP. Along with cereals, pea and lentil, bitter vetch has become definitely associated with the start of the 'agricultural revolution' in the Old World. Bitter vetch entered Europe in its south-east regions and progressed into its interior via Danube. Its distribution was rapid, since the available evidence reveals its presence in remote places at similar periods. Recently the first success has been obtained in the extraction of ancient DNA from charred bitter vetch seeds. The linguistic evidence supports the fact that most of Eurasian peoples have their own words denoting bitter vetch, meaning that its cultivation preceded the diversification of their own proto-languages.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - A note on the earliest distribution, cultivation and genetic changes in bitter vetch (vicia ervilia) in ancient Europe
EP  - 11
IS  - 1
SP  - 1
VL  - 47
DO  - 10.2298/GENSR1501001M
ER  - 

@article{
author = "Mikić, Aleksandar and Medović, Aleksandar and Jovanović, Živko and Stanisavljević, Nemanja",
year = "2015",
abstract = "Bitter vetch (Vicia ervilia (L.) Willd.) was a part of the everyday diet of the Eurasian Neanderthal population and the modern human Palaeolithic hunter-gatherers at the end of the last Ice Age. The major criteria to determine the domestication in bitter vetch and other ancient grain legumes are non-dehiscent pods, larger seed size and smooth seed testa. Bitter vetch seeds were found among the earliest findings of cultivated crops at the site of Tell El-Kerkh, Syria, from 10th millennium BP. Along with cereals, pea and lentil, bitter vetch has become definitely associated with the start of the 'agricultural revolution' in the Old World. Bitter vetch entered Europe in its south-east regions and progressed into its interior via Danube. Its distribution was rapid, since the available evidence reveals its presence in remote places at similar periods. Recently the first success has been obtained in the extraction of ancient DNA from charred bitter vetch seeds. The linguistic evidence supports the fact that most of Eurasian peoples have their own words denoting bitter vetch, meaning that its cultivation preceded the diversification of their own proto-languages.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "A note on the earliest distribution, cultivation and genetic changes in bitter vetch (vicia ervilia) in ancient Europe",
pages = "11-1",
number = "1",
volume = "47",
doi = "10.2298/GENSR1501001M"
}

Mikić, A., Medović, A., Jovanović, Ž.,& Stanisavljević, N.. (2015). A note on the earliest distribution, cultivation and genetic changes in bitter vetch (vicia ervilia) in ancient Europe. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 47(1), 1-11.
https://doi.org/10.2298/GENSR1501001M

Mikić A, Medović A, Jovanović Ž, Stanisavljević N. A note on the earliest distribution, cultivation and genetic changes in bitter vetch (vicia ervilia) in ancient Europe. in Genetika-Belgrade. 2015;47(1):1-11.
doi:10.2298/GENSR1501001M .

Mikić, Aleksandar, Medović, Aleksandar, Jovanović, Živko, Stanisavljević, Nemanja, "A note on the earliest distribution, cultivation and genetic changes in bitter vetch (vicia ervilia) in ancient Europe" in Genetika-Belgrade, 47, no. 1 (2015):1-11,
https://doi.org/10.2298/GENSR1501001M . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Ilić, Marija
AU  - Jovanović, Živko
AU  - Cupić, Tihomir
AU  - Dabić, Dragana C.
AU  - Natić, Maja M.
AU  - Tesić, Zivoslav Lj.
AU  - Radović, Svetlana S.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/832
AB  - The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu's method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antioxidant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity
EP  - 840
IS  - 3
SP  - 829
VL  - 67
DO  - 10.2298/ABS141204042S
ER  - 

@article{
author = "Stanisavljević, Nemanja and Ilić, Marija and Jovanović, Živko and Cupić, Tihomir and Dabić, Dragana C. and Natić, Maja M. and Tesić, Zivoslav Lj. and Radović, Svetlana S.",
year = "2015",
abstract = "The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu's method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antioxidant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity",
pages = "840-829",
number = "3",
volume = "67",
doi = "10.2298/ABS141204042S"
}

Stanisavljević, N., Ilić, M., Jovanović, Ž., Cupić, T., Dabić, D. C., Natić, M. M., Tesić, Z. Lj.,& Radović, S. S.. (2015). Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 67(3), 829-840.
https://doi.org/10.2298/ABS141204042S

Stanisavljević N, Ilić M, Jovanović Ž, Cupić T, Dabić DC, Natić MM, Tesić ZL, Radović SS. Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity. in Archives of Biological Sciences. 2015;67(3):829-840.
doi:10.2298/ABS141204042S .

Stanisavljević, Nemanja, Ilić, Marija, Jovanović, Živko, Cupić, Tihomir, Dabić, Dragana C., Natić, Maja M., Tesić, Zivoslav Lj., Radović, Svetlana S., "Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity" in Archives of Biological Sciences, 67, no. 3 (2015):829-840,
https://doi.org/10.2298/ABS141204042S . .

TY  - JOUR
AU  - Aleksić, Jelena M.
AU  - Miljuš-Đukić, Jovanka
AU  - Jovanović, Živko
AU  - Tomić, Branko
AU  - Banović Đeri, Bojana
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/732
AB  - We performed in silico PCR analyses utilizing complete mitochondrial (mtDNA) genome sequences of faba bean (Vicia faba) and two related species, Vigna angularis and Vigna radiata, currently available in GenBank, to infer whether 15 published universal primer pairs for amplification of all 14 cis-spliced introns in genes of NADH subunits (nad genes) are suitable for V. faba and related species. Then, we tested via PCR reactions whether seven out of 15 primer pairs would generate PCR products suitable for further manipulation in 16 genotypes of V. faba representing all botanical varieties of this species (major, minor, equina and subsp. paucijuga) of various levels of improvement (traditional and improved cultivars) originating from Europe, Africa, Asia and south America. We provide new PCR primers for amplification of nad1 intron 2/3 in V. faba, and demonstrate intraspecific variability in primary nucleotide sequences at this locus. Based on outcomes of both in silico predictions and PCR amplification, we report a set of PCR primers for amplification of five introns in nad genes that are promising molecular tools for future phylogeographic and other studies in this species for which unambiguous data on wild ancestors, centre of origin and domestication are lacking.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Molecular tools for utilization of mitochondrial diversity in faba bean (vicia faba)
EP  - 762
IS  - 3
SP  - 745
VL  - 46
DO  - 10.2298/GENSR1403745A
ER  - 

@article{
author = "Aleksić, Jelena M. and Miljuš-Đukić, Jovanka and Jovanović, Živko and Tomić, Branko and Banović Đeri, Bojana",
year = "2014",
abstract = "We performed in silico PCR analyses utilizing complete mitochondrial (mtDNA) genome sequences of faba bean (Vicia faba) and two related species, Vigna angularis and Vigna radiata, currently available in GenBank, to infer whether 15 published universal primer pairs for amplification of all 14 cis-spliced introns in genes of NADH subunits (nad genes) are suitable for V. faba and related species. Then, we tested via PCR reactions whether seven out of 15 primer pairs would generate PCR products suitable for further manipulation in 16 genotypes of V. faba representing all botanical varieties of this species (major, minor, equina and subsp. paucijuga) of various levels of improvement (traditional and improved cultivars) originating from Europe, Africa, Asia and south America. We provide new PCR primers for amplification of nad1 intron 2/3 in V. faba, and demonstrate intraspecific variability in primary nucleotide sequences at this locus. Based on outcomes of both in silico predictions and PCR amplification, we report a set of PCR primers for amplification of five introns in nad genes that are promising molecular tools for future phylogeographic and other studies in this species for which unambiguous data on wild ancestors, centre of origin and domestication are lacking.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Molecular tools for utilization of mitochondrial diversity in faba bean (vicia faba)",
pages = "762-745",
number = "3",
volume = "46",
doi = "10.2298/GENSR1403745A"
}

Aleksić, J. M., Miljuš-Đukić, J., Jovanović, Ž., Tomić, B.,& Banović Đeri, B.. (2014). Molecular tools for utilization of mitochondrial diversity in faba bean (vicia faba). in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 46(3), 745-762.
https://doi.org/10.2298/GENSR1403745A

Aleksić JM, Miljuš-Đukić J, Jovanović Ž, Tomić B, Banović Đeri B. Molecular tools for utilization of mitochondrial diversity in faba bean (vicia faba). in Genetika-Belgrade. 2014;46(3):745-762.
doi:10.2298/GENSR1403745A .

Aleksić, Jelena M., Miljuš-Đukić, Jovanka, Jovanović, Živko, Tomić, Branko, Banović Đeri, Bojana, "Molecular tools for utilization of mitochondrial diversity in faba bean (vicia faba)" in Genetika-Belgrade, 46, no. 3 (2014):745-762,
https://doi.org/10.2298/GENSR1403745A . .

TY  - JOUR
AU  - Mikić, Aleksandar
AU  - Medović, Aleksandar
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/725
AB  - This short communication intends to discuss various models and ideas about the very beginnings of plant domestication in the Old World with a specific emphasis on pea (Pisum sativum L.) as one of the most significant crops in the human civilization. It presents a selection of some recent achievements and challenges in studying the earliest days of this important grain legume species by balanced contributions from archaeobotany, paleogenetics and historical linguistics. It will hopefully stimulate a fruitful interdisciplinary discussion and provide a basis for new experimental approaches.
PB  - Springer, Dordrecht
T2  - Genetic Resources and Crop Evolution
T1  - Integrating archaeobotany, paleogenetics and historical linguistics may cast more light onto crop domestication: the case of pea (Pisum sativum)
EP  - 892
IS  - 5
SP  - 887
VL  - 61
DO  - 10.1007/s10722-014-0102-9
ER  - 

@article{
author = "Mikić, Aleksandar and Medović, Aleksandar and Jovanović, Živko and Stanisavljević, Nemanja",
year = "2014",
abstract = "This short communication intends to discuss various models and ideas about the very beginnings of plant domestication in the Old World with a specific emphasis on pea (Pisum sativum L.) as one of the most significant crops in the human civilization. It presents a selection of some recent achievements and challenges in studying the earliest days of this important grain legume species by balanced contributions from archaeobotany, paleogenetics and historical linguistics. It will hopefully stimulate a fruitful interdisciplinary discussion and provide a basis for new experimental approaches.",
publisher = "Springer, Dordrecht",
journal = "Genetic Resources and Crop Evolution",
title = "Integrating archaeobotany, paleogenetics and historical linguistics may cast more light onto crop domestication: the case of pea (Pisum sativum)",
pages = "892-887",
number = "5",
volume = "61",
doi = "10.1007/s10722-014-0102-9"
}

Mikić, A., Medović, A., Jovanović, Ž.,& Stanisavljević, N.. (2014). Integrating archaeobotany, paleogenetics and historical linguistics may cast more light onto crop domestication: the case of pea (Pisum sativum). in Genetic Resources and Crop Evolution
Springer, Dordrecht., 61(5), 887-892.
https://doi.org/10.1007/s10722-014-0102-9

Mikić A, Medović A, Jovanović Ž, Stanisavljević N. Integrating archaeobotany, paleogenetics and historical linguistics may cast more light onto crop domestication: the case of pea (Pisum sativum). in Genetic Resources and Crop Evolution. 2014;61(5):887-892.
doi:10.1007/s10722-014-0102-9 .

Mikić, Aleksandar, Medović, Aleksandar, Jovanović, Živko, Stanisavljević, Nemanja, "Integrating archaeobotany, paleogenetics and historical linguistics may cast more light onto crop domestication: the case of pea (Pisum sativum)" in Genetic Resources and Crop Evolution, 61, no. 5 (2014):887-892,
https://doi.org/10.1007/s10722-014-0102-9 . .

TY  - JOUR
AU  - Smykal, Petr
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Zlatković, Bojan
AU  - Cupina, Branko
AU  - Đorđević, Vuk
AU  - Mikić, Aleksandar
AU  - Medović, Aleksandar
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/755
AB  - The development of agriculture was a key turning point in human history, a central part of which was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. First domesticated in the Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. In this study, we present a molecular analysis of ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. Four selected chloroplast DNA loci (trnSG, trnK, matK and rbcL) amplified in six fragments of 128-340 bp with a total length of 1,329 bp were successfully recovered in order to distinguish between cultivated and wild gathered pea. Based on identified mutations, the results showed that genuine aDNA was analyzed. Moreover, DNA analysis resulted in placing the ancient sample at an intermediate position between extant cultivated [Pisum sativum L. and wild P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.]. Consequently, based on a combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of colored flower and pigmented testa, similar to today's fodder pea (P. sativum subsp. sativum var. arvense (L.) Poir.), possibly of winter type. This is the first report of successful aDNA extraction and analysis from any legume species thus far. The implications for pea domestication are discussed here.
PB  - Springer, Dordrecht
T2  - Genetic Resources and Crop Evolution
T1  - A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication
EP  - 1544
IS  - 8
SP  - 1533
VL  - 61
DO  - 10.1007/s10722-014-0128-z
ER  - 

@article{
author = "Smykal, Petr and Jovanović, Živko and Stanisavljević, Nemanja and Zlatković, Bojan and Cupina, Branko and Đorđević, Vuk and Mikić, Aleksandar and Medović, Aleksandar",
year = "2014",
abstract = "The development of agriculture was a key turning point in human history, a central part of which was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. First domesticated in the Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. In this study, we present a molecular analysis of ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. Four selected chloroplast DNA loci (trnSG, trnK, matK and rbcL) amplified in six fragments of 128-340 bp with a total length of 1,329 bp were successfully recovered in order to distinguish between cultivated and wild gathered pea. Based on identified mutations, the results showed that genuine aDNA was analyzed. Moreover, DNA analysis resulted in placing the ancient sample at an intermediate position between extant cultivated [Pisum sativum L. and wild P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.]. Consequently, based on a combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of colored flower and pigmented testa, similar to today's fodder pea (P. sativum subsp. sativum var. arvense (L.) Poir.), possibly of winter type. This is the first report of successful aDNA extraction and analysis from any legume species thus far. The implications for pea domestication are discussed here.",
publisher = "Springer, Dordrecht",
journal = "Genetic Resources and Crop Evolution",
title = "A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication",
pages = "1544-1533",
number = "8",
volume = "61",
doi = "10.1007/s10722-014-0128-z"
}

Smykal, P., Jovanović, Ž., Stanisavljević, N., Zlatković, B., Cupina, B., Đorđević, V., Mikić, A.,& Medović, A.. (2014). A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication. in Genetic Resources and Crop Evolution
Springer, Dordrecht., 61(8), 1533-1544.
https://doi.org/10.1007/s10722-014-0128-z

Smykal P, Jovanović Ž, Stanisavljević N, Zlatković B, Cupina B, Đorđević V, Mikić A, Medović A. A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication. in Genetic Resources and Crop Evolution. 2014;61(8):1533-1544.
doi:10.1007/s10722-014-0128-z .

Smykal, Petr, Jovanović, Živko, Stanisavljević, Nemanja, Zlatković, Bojan, Cupina, Branko, Đorđević, Vuk, Mikić, Aleksandar, Medović, Aleksandar, "A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication" in Genetic Resources and Crop Evolution, 61, no. 8 (2014):1533-1544,
https://doi.org/10.1007/s10722-014-0128-z . .

TY  - JOUR
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Mikić, Aleksandar
AU  - Radović, Svetlana
AU  - Maksimović, Vesna R.
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/742
AB  - MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at post-transcriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that they could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame reflects the changes as close as possible to the changes where water stress causes visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target - genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. To the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) did not change in roots and shoots of water-stressed plants, compared to control (well) hydrated plants. This suggests that COX5b is not the target of miR398, or that its expression is regulated by some other mechanism. P-1B-ATPase expression increased during water deficit only in the shoots of pea; in the roots there were no changes in expression. Our results help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
T2  - Plant Physiology and Biochemistry
T1  - Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.)
EP  - 31
SP  - 26
VL  - 83
DO  - 10.1016/j.plaphy.2014.07.008
ER  - 

@article{
author = "Jovanović, Živko and Stanisavljević, Nemanja and Mikić, Aleksandar and Radović, Svetlana and Maksimović, Vesna R.",
year = "2014",
abstract = "MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at post-transcriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that they could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame reflects the changes as close as possible to the changes where water stress causes visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target - genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. To the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) did not change in roots and shoots of water-stressed plants, compared to control (well) hydrated plants. This suggests that COX5b is not the target of miR398, or that its expression is regulated by some other mechanism. P-1B-ATPase expression increased during water deficit only in the shoots of pea; in the roots there were no changes in expression. Our results help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris",
journal = "Plant Physiology and Biochemistry",
title = "Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.)",
pages = "31-26",
volume = "83",
doi = "10.1016/j.plaphy.2014.07.008"
}

Jovanović, Ž., Stanisavljević, N., Mikić, A., Radović, S.,& Maksimović, V. R.. (2014). Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.). in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Paris., 83, 26-31.
https://doi.org/10.1016/j.plaphy.2014.07.008

Jovanović Ž, Stanisavljević N, Mikić A, Radović S, Maksimović VR. Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.). in Plant Physiology and Biochemistry. 2014;83:26-31.
doi:10.1016/j.plaphy.2014.07.008 .

Jovanović, Živko, Stanisavljević, Nemanja, Mikić, Aleksandar, Radović, Svetlana, Maksimović, Vesna R., "Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.)" in Plant Physiology and Biochemistry, 83 (2014):26-31,
https://doi.org/10.1016/j.plaphy.2014.07.008 . .

TY  - JOUR
AU  - Rat, M.
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Radak, B.
AU  - Bokić, B.
AU  - Radović, S.
AU  - Anačkov, G.
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/758
AB  - We report an efficient, simple and cost-effective protocol for the isolation of genomic DNA from Ornithogalum species. Our modification of the standard CTAB protocol includes two polyphenol adsorbents (insoluble PVPP and activated charcoal), high NaCl concentrations (4 M) for removing polysaccharides, and addition of phenol to remove proteins and other contaminants. DNA yield obtained with our protocol was 223 and 312 μg DNA g-1 of dry leaf tissue. The absorbance ratio 260/280 nm was 1.879 (O. refractum) and 1.753 (O. sibthorpii), and the absorbance ratio 260/230 nm was 1.779 (O. refractum) and 1.545 (O. sibthorpii), revealing lack of contamination. PCR amplifications of one nuclear marker (26S rDNA) indicated that this DNA isolation protocol may be used for Ornithogalum plants containing many interfering compounds for further analyses in population genetics and phylogeographic studies.
PB  - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
T2  - Botanica Serbica
T1  - A simple and efficient DNA isolation method for Ornithogalum L. species (Hyacinthaceae, Asparagales)
EP  - 190
IS  - 1
SP  - 185
VL  - 38
UR  - https://hdl.handle.net/21.15107/rcub_imagine_758
ER  - 

@article{
author = "Rat, M. and Jovanović, Živko and Stanisavljević, Nemanja and Radak, B. and Bokić, B. and Radović, S. and Anačkov, G.",
year = "2014",
abstract = "We report an efficient, simple and cost-effective protocol for the isolation of genomic DNA from Ornithogalum species. Our modification of the standard CTAB protocol includes two polyphenol adsorbents (insoluble PVPP and activated charcoal), high NaCl concentrations (4 M) for removing polysaccharides, and addition of phenol to remove proteins and other contaminants. DNA yield obtained with our protocol was 223 and 312 μg DNA g-1 of dry leaf tissue. The absorbance ratio 260/280 nm was 1.879 (O. refractum) and 1.753 (O. sibthorpii), and the absorbance ratio 260/230 nm was 1.779 (O. refractum) and 1.545 (O. sibthorpii), revealing lack of contamination. PCR amplifications of one nuclear marker (26S rDNA) indicated that this DNA isolation protocol may be used for Ornithogalum plants containing many interfering compounds for further analyses in population genetics and phylogeographic studies.",
publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd",
journal = "Botanica Serbica",
title = "A simple and efficient DNA isolation method for Ornithogalum L. species (Hyacinthaceae, Asparagales)",
pages = "190-185",
number = "1",
volume = "38",
url = "https://hdl.handle.net/21.15107/rcub_imagine_758"
}

Rat, M., Jovanović, Ž., Stanisavljević, N., Radak, B., Bokić, B., Radović, S.,& Anačkov, G.. (2014). A simple and efficient DNA isolation method for Ornithogalum L. species (Hyacinthaceae, Asparagales). in Botanica Serbica
Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 38(1), 185-190.
https://hdl.handle.net/21.15107/rcub_imagine_758

Rat M, Jovanović Ž, Stanisavljević N, Radak B, Bokić B, Radović S, Anačkov G. A simple and efficient DNA isolation method for Ornithogalum L. species (Hyacinthaceae, Asparagales). in Botanica Serbica. 2014;38(1):185-190.
https://hdl.handle.net/21.15107/rcub_imagine_758 .

Rat, M., Jovanović, Živko, Stanisavljević, Nemanja, Radak, B., Bokić, B., Radović, S., Anačkov, G., "A simple and efficient DNA isolation method for Ornithogalum L. species (Hyacinthaceae, Asparagales)" in Botanica Serbica, 38, no. 1 (2014):185-190,
https://hdl.handle.net/21.15107/rcub_imagine_758 .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Jovanović, Živko
AU  - Cupić, Tihomir
AU  - Lukić, Jovanka
AU  - Miljus Dukić, Jovanka
AU  - Radović, Svetlana
AU  - Mikić, Aleksandar
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/626
AB  - Antioxidant activities were studied in methanolic and water extracts of nonprocessed, cooked and in vitro enzymatically digested seed flour, as well as in total protein hydrolysates and small peptide fractions ( lt 3 and  lt 10kDa) of three pea and five grass pea cultivars. The antioxidative properties were determined by three spectrophotometric methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, Folin-Ciocalteu (FC) reducing capacity assay and H2O2 scavenging. We also applied one luminometric assay for hydroxyl radical scavenging. The study showed that cooking and enzymatic digestion strongly enhanced the release of phenolic compounds in methanolic extracts of four analysed cultivars. Scavenging activity against DPPH radical, hydroxyl radical and hydrogen peroxide was increased in majority of analysed flour specimens subjected to processing. Our findings indicate that, besides the phenolic compounds, the small peptide fraction, especially the MW  lt 3kDa, in methanolic and aqueous extracts of cooked and digested seed flour significantly contribute to free radical and hydrogen peroxide scavenging activity in all investigated cultivars. Our data strongly suggest that simple cooking treatment and in vitro digestion of seed flour applied prior to extraction with methanol could improve antioxidative activity of obtained extracts.
PB  - Wiley-Blackwell, Hoboken
T2  - International Journal of Food Science and Technology
T1  - Extractability of antioxidants from legume seed flour after cooking and in vitro gastrointestinal digestion in comparison with methanolic extraction of the unprocessed flour
EP  - 2104
IS  - 10
SP  - 2096
VL  - 48
DO  - 10.1111/ijfs.12191
ER  - 

@article{
author = "Stanisavljević, Nemanja and Jovanović, Živko and Cupić, Tihomir and Lukić, Jovanka and Miljus Dukić, Jovanka and Radović, Svetlana and Mikić, Aleksandar",
year = "2013",
abstract = "Antioxidant activities were studied in methanolic and water extracts of nonprocessed, cooked and in vitro enzymatically digested seed flour, as well as in total protein hydrolysates and small peptide fractions ( lt 3 and  lt 10kDa) of three pea and five grass pea cultivars. The antioxidative properties were determined by three spectrophotometric methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, Folin-Ciocalteu (FC) reducing capacity assay and H2O2 scavenging. We also applied one luminometric assay for hydroxyl radical scavenging. The study showed that cooking and enzymatic digestion strongly enhanced the release of phenolic compounds in methanolic extracts of four analysed cultivars. Scavenging activity against DPPH radical, hydroxyl radical and hydrogen peroxide was increased in majority of analysed flour specimens subjected to processing. Our findings indicate that, besides the phenolic compounds, the small peptide fraction, especially the MW  lt 3kDa, in methanolic and aqueous extracts of cooked and digested seed flour significantly contribute to free radical and hydrogen peroxide scavenging activity in all investigated cultivars. Our data strongly suggest that simple cooking treatment and in vitro digestion of seed flour applied prior to extraction with methanol could improve antioxidative activity of obtained extracts.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "International Journal of Food Science and Technology",
title = "Extractability of antioxidants from legume seed flour after cooking and in vitro gastrointestinal digestion in comparison with methanolic extraction of the unprocessed flour",
pages = "2104-2096",
number = "10",
volume = "48",
doi = "10.1111/ijfs.12191"
}

Stanisavljević, N., Jovanović, Ž., Cupić, T., Lukić, J., Miljus Dukić, J., Radović, S.,& Mikić, A.. (2013). Extractability of antioxidants from legume seed flour after cooking and in vitro gastrointestinal digestion in comparison with methanolic extraction of the unprocessed flour. in International Journal of Food Science and Technology
Wiley-Blackwell, Hoboken., 48(10), 2096-2104.
https://doi.org/10.1111/ijfs.12191

Stanisavljević N, Jovanović Ž, Cupić T, Lukić J, Miljus Dukić J, Radović S, Mikić A. Extractability of antioxidants from legume seed flour after cooking and in vitro gastrointestinal digestion in comparison with methanolic extraction of the unprocessed flour. in International Journal of Food Science and Technology. 2013;48(10):2096-2104.
doi:10.1111/ijfs.12191 .

Stanisavljević, Nemanja, Jovanović, Živko, Cupić, Tihomir, Lukić, Jovanka, Miljus Dukić, Jovanka, Radović, Svetlana, Mikić, Aleksandar, "Extractability of antioxidants from legume seed flour after cooking and in vitro gastrointestinal digestion in comparison with methanolic extraction of the unprocessed flour" in International Journal of Food Science and Technology, 48, no. 10 (2013):2096-2104,
https://doi.org/10.1111/ijfs.12191 . .

TY  - JOUR
AU  - Miljuš-Đukić, Jovanka
AU  - Stanisavljević, Nemanja
AU  - Radović, S.
AU  - Jovanović, Živko
AU  - Mikić, A.
AU  - Maksimović, Vesna R.
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/688
AB  - Soil salinity is one of the major abiotic stresses leading to crops yield failure. For investigation of salinity response in pea, in vitro cultures of three pea species (Pisum arvense, P. sativum and P. fulvum) were induced. Regenerated shoots of these pea species were grown on a medium with moderate and high NaCl concentrations (75, 120, 150 and 200 mM NaCl) to compare the antioxidative capacity in response to salt stress. Growth reduction was observed in all species and it correlated with the increase of NaCl concentration. After four weeks of treatment, the crude tissue extracts were used for measuring the stress response parameters. Level of lipid peroxidation increased in all three species, but in P. fulvum the effect of NaCl was less pronounced. Total chlorophyll and phenolic content showed differences among the three species, while the DPPH-scavenging activity was the most evident in P. fulvum. The northern blot analysis of the miRNA398 expression showed a similar pattern between P. sativum and P. arvense but different to that of P. fulvum, as in P. fulvum miRNA 398 was only expressed at moderate NaCl concentration, with total inhibition at high concentrations. On the basis of these results it can be concluded that P. fulvum could represent a gene pool for improving P. sativum and P. arvense stress defence capacity.
T2  - Australian Journal of Crop Science
T1  - Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression
EP  - 2153
IS  - 13
SP  - 2145
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_imagine_688
ER  - 

@article{
author = "Miljuš-Đukić, Jovanka and Stanisavljević, Nemanja and Radović, S. and Jovanović, Živko and Mikić, A. and Maksimović, Vesna R.",
year = "2013",
abstract = "Soil salinity is one of the major abiotic stresses leading to crops yield failure. For investigation of salinity response in pea, in vitro cultures of three pea species (Pisum arvense, P. sativum and P. fulvum) were induced. Regenerated shoots of these pea species were grown on a medium with moderate and high NaCl concentrations (75, 120, 150 and 200 mM NaCl) to compare the antioxidative capacity in response to salt stress. Growth reduction was observed in all species and it correlated with the increase of NaCl concentration. After four weeks of treatment, the crude tissue extracts were used for measuring the stress response parameters. Level of lipid peroxidation increased in all three species, but in P. fulvum the effect of NaCl was less pronounced. Total chlorophyll and phenolic content showed differences among the three species, while the DPPH-scavenging activity was the most evident in P. fulvum. The northern blot analysis of the miRNA398 expression showed a similar pattern between P. sativum and P. arvense but different to that of P. fulvum, as in P. fulvum miRNA 398 was only expressed at moderate NaCl concentration, with total inhibition at high concentrations. On the basis of these results it can be concluded that P. fulvum could represent a gene pool for improving P. sativum and P. arvense stress defence capacity.",
journal = "Australian Journal of Crop Science",
title = "Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression",
pages = "2153-2145",
number = "13",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_imagine_688"
}

Miljuš-Đukić, J., Stanisavljević, N., Radović, S., Jovanović, Ž., Mikić, A.,& Maksimović, V. R.. (2013). Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression. in Australian Journal of Crop Science, 7(13), 2145-2153.
https://hdl.handle.net/21.15107/rcub_imagine_688

Miljuš-Đukić J, Stanisavljević N, Radović S, Jovanović Ž, Mikić A, Maksimović VR. Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression. in Australian Journal of Crop Science. 2013;7(13):2145-2153.
https://hdl.handle.net/21.15107/rcub_imagine_688 .

Miljuš-Đukić, Jovanka, Stanisavljević, Nemanja, Radović, S., Jovanović, Živko, Mikić, A., Maksimović, Vesna R., "Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression" in Australian Journal of Crop Science, 7, no. 13 (2013):2145-2153,
https://hdl.handle.net/21.15107/rcub_imagine_688 .

TY  - JOUR
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Mikić, A.
AU  - Radović, S.
AU  - Maksimović, Vesna R.
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/684
AB  - Protein pea (Pisum sativum L.) is an agronomic type of pea introduced in the region of modern Serbia in the early eighties of the last century. In this study, a new variety NS MRAZ developed by pedigree selection in 2011 was used. Two-week-old plants were subjected to drought stress by withholding irrigation for 7 and 10 days, and rehydrated for one day. Stress effects were monitored by determination of relative water content (RWC), lipid peroxidation and accumulation of reactive oxygen species (ROS).We isolated partial cDNA of Pisum sativum DREB2A, namely PsDREB2A (HM229349), which belongs to the DREB gene family. Bioinformatics analyses showed high similarity with DREB2A gene from model legume Medicago truncataula. The relationship between the expression profile of PsDREB2A gene and water stress was assayed by quantitative real time PCR in pea roots and leaves. According to obtained results, it is evident that loss of water content strongly induced accumulation of ROS and lipid peroxidation in pea plants. The expression of PsDREB2A in pea roots increased with water content decrease, reaching maximum after 10 days of dehydration (2 fold higher than in control plants). On the other hand, in the pea leaves, the highest level of expression was observed after 7 days of dehydration (60% higher than in control). Observed tissue-specific expression profile of PsDREB2A suggests complex regulation and the role of this transcription factor in pea drought response. In addition, we can conclude that this pea (var." NS MRAZ") is a drought sensitive plant.
T2  - Australian Journal of Crop Science
T1  - The expression of drought responsive element binding protein (DREB2A) related gene from pea (Oisum sativum L.) as affected by water stress
EP  - 1596
IS  - 10
SP  - 1590
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_imagine_684
ER  - 

@article{
author = "Jovanović, Živko and Stanisavljević, Nemanja and Mikić, A. and Radović, S. and Maksimović, Vesna R.",
year = "2013",
abstract = "Protein pea (Pisum sativum L.) is an agronomic type of pea introduced in the region of modern Serbia in the early eighties of the last century. In this study, a new variety NS MRAZ developed by pedigree selection in 2011 was used. Two-week-old plants were subjected to drought stress by withholding irrigation for 7 and 10 days, and rehydrated for one day. Stress effects were monitored by determination of relative water content (RWC), lipid peroxidation and accumulation of reactive oxygen species (ROS).We isolated partial cDNA of Pisum sativum DREB2A, namely PsDREB2A (HM229349), which belongs to the DREB gene family. Bioinformatics analyses showed high similarity with DREB2A gene from model legume Medicago truncataula. The relationship between the expression profile of PsDREB2A gene and water stress was assayed by quantitative real time PCR in pea roots and leaves. According to obtained results, it is evident that loss of water content strongly induced accumulation of ROS and lipid peroxidation in pea plants. The expression of PsDREB2A in pea roots increased with water content decrease, reaching maximum after 10 days of dehydration (2 fold higher than in control plants). On the other hand, in the pea leaves, the highest level of expression was observed after 7 days of dehydration (60% higher than in control). Observed tissue-specific expression profile of PsDREB2A suggests complex regulation and the role of this transcription factor in pea drought response. In addition, we can conclude that this pea (var." NS MRAZ") is a drought sensitive plant.",
journal = "Australian Journal of Crop Science",
title = "The expression of drought responsive element binding protein (DREB2A) related gene from pea (Oisum sativum L.) as affected by water stress",
pages = "1596-1590",
number = "10",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_imagine_684"
}

Jovanović, Ž., Stanisavljević, N., Mikić, A., Radović, S.,& Maksimović, V. R.. (2013). The expression of drought responsive element binding protein (DREB2A) related gene from pea (Oisum sativum L.) as affected by water stress. in Australian Journal of Crop Science, 7(10), 1590-1596.
https://hdl.handle.net/21.15107/rcub_imagine_684

Jovanović Ž, Stanisavljević N, Mikić A, Radović S, Maksimović VR. The expression of drought responsive element binding protein (DREB2A) related gene from pea (Oisum sativum L.) as affected by water stress. in Australian Journal of Crop Science. 2013;7(10):1590-1596.
https://hdl.handle.net/21.15107/rcub_imagine_684 .

Jovanović, Živko, Stanisavljević, Nemanja, Mikić, A., Radović, S., Maksimović, Vesna R., "The expression of drought responsive element binding protein (DREB2A) related gene from pea (Oisum sativum L.) as affected by water stress" in Australian Journal of Crop Science, 7, no. 10 (2013):1590-1596,
https://hdl.handle.net/21.15107/rcub_imagine_684 .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Savić, Jelena
AU  - Jovanović, Živko
AU  - Miljuš-Đukić, Jovanka
AU  - Radović, Svetlana
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/580
AB  - Shoot cultures of rare Balkan hyperaccumulating species Alyssum markgrafii were subjected to high nickel concentrations of 1, 3, 5, and 8 mM. The effects of graded nickel concentrations on toxicity, pigments, and several components of plant antioxidative defense system were characterized. Toxic effects of excess nickel were observed through slower growth and biomass decrease, together with increased reactive oxygen species (ROS) production and lipid peroxidation. Nickel exposure decreased chlorophyll a, b, total chlorophyll as well as carotenoid concentration. Addition of sodium benzoate, potent ROS scavenger, showed concentration-dependent disturbing effect on nickel hyperaccumulation, lowering the content of accumulated nickel in A. markgrafii shoots. General reducing power represented by low molecular weight antioxidants and phenols was inversely correlated with nickel concentration. Among the investigated antioxidative enzymes, POD seems to play important role in ROS level regulation upon excessive nickel in medium.
PB  - Springer Heidelberg, Heidelberg
T2  - Acta Physiologiae Plantarum
T1  - Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level
EP  - 2006
IS  - 5
SP  - 1997
VL  - 34
DO  - 10.1007/s11738-012-1001-3
ER  - 

@article{
author = "Stanisavljević, Nemanja and Savić, Jelena and Jovanović, Živko and Miljuš-Đukić, Jovanka and Radović, Svetlana and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2012",
abstract = "Shoot cultures of rare Balkan hyperaccumulating species Alyssum markgrafii were subjected to high nickel concentrations of 1, 3, 5, and 8 mM. The effects of graded nickel concentrations on toxicity, pigments, and several components of plant antioxidative defense system were characterized. Toxic effects of excess nickel were observed through slower growth and biomass decrease, together with increased reactive oxygen species (ROS) production and lipid peroxidation. Nickel exposure decreased chlorophyll a, b, total chlorophyll as well as carotenoid concentration. Addition of sodium benzoate, potent ROS scavenger, showed concentration-dependent disturbing effect on nickel hyperaccumulation, lowering the content of accumulated nickel in A. markgrafii shoots. General reducing power represented by low molecular weight antioxidants and phenols was inversely correlated with nickel concentration. Among the investigated antioxidative enzymes, POD seems to play important role in ROS level regulation upon excessive nickel in medium.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Acta Physiologiae Plantarum",
title = "Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level",
pages = "2006-1997",
number = "5",
volume = "34",
doi = "10.1007/s11738-012-1001-3"
}

Stanisavljević, N., Savić, J., Jovanović, Ž., Miljuš-Đukić, J., Radović, S., Vinterhalter, D.,& Vinterhalter, B.. (2012). Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level. in Acta Physiologiae Plantarum
Springer Heidelberg, Heidelberg., 34(5), 1997-2006.
https://doi.org/10.1007/s11738-012-1001-3

Stanisavljević N, Savić J, Jovanović Ž, Miljuš-Đukić J, Radović S, Vinterhalter D, Vinterhalter B. Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level. in Acta Physiologiae Plantarum. 2012;34(5):1997-2006.
doi:10.1007/s11738-012-1001-3 .

Stanisavljević, Nemanja, Savić, Jelena, Jovanović, Živko, Miljuš-Đukić, Jovanka, Radović, Svetlana, Vinterhalter, Dragan, Vinterhalter, Branka, "Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level" in Acta Physiologiae Plantarum, 34, no. 5 (2012):1997-2006,
https://doi.org/10.1007/s11738-012-1001-3 . .

TY  - JOUR
AU  - Trudić, Branislav
AU  - Radović, Svetlana
AU  - Galović, Vladislava
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/546
AB  - Sve učestalije i intenzivnije promene faktora spoljašnje sredine deluju na biljke kao stres. Suočene sa ovim promenama biljke su razvile mehanizme tolerancije i adaptacije, koji im omogućavaju opstanak. Ovi mehanizmi funkcionišu na morfološkom, fiziološkom, biohemijskom i molekularnom nivou, međusobno su povezani, regulisani i usklađeni u integrisani sistem odgovora na promene u životnoj sredini. Mnogi ključni regulatorni i funkcionalni molekuli koji učestvuju u odgovoru na abiotički stres su identifikovani i time je otvorena mogućnost primene novih strategija u upravljanju stresom. Tema ovog rada su molekularni i biohemijski mehanizmi odgovora drvenastih vrsta biljaka na različite tipove abiotičkog stresa, sa posebnim osvrtom na topole.
AB  - More frequent and more intense changes in environmental factors act as a stress on the plants. Faced with these changes, plants have developed mechanisms of tolerance and adaptation, which enable them to survive. These mechanisms operate on the morphological, physiological, biochemical and molecular level, are interconnected, regulated and coordinated in an integrated system response to changes in the environment. Many key regulatory and functional molecules involved in the response to abiotic stress are identified, thus opening the possibility for implementation of new strategies in the stress management. This paper outlines the molecular and biochemical mechanisms of response of woody plants to different types of abiotic stress, with special emphasis on poplars.
PB  - Univerzitet u Novom Sadu - Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad
T2  - Topola
T1  - Molekularni mehanizmi odgovora drvenastih vrsta biljaka na abiotički stres
T1  - Molecular mechanisms of woody plant species abiotic stress response
EP  - 86
IS  - 189-190
SP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_imagine_546
ER  - 

@article{
author = "Trudić, Branislav and Radović, Svetlana and Galović, Vladislava and Jovanović, Živko and Stanisavljević, Nemanja",
year = "2012",
abstract = "Sve učestalije i intenzivnije promene faktora spoljašnje sredine deluju na biljke kao stres. Suočene sa ovim promenama biljke su razvile mehanizme tolerancije i adaptacije, koji im omogućavaju opstanak. Ovi mehanizmi funkcionišu na morfološkom, fiziološkom, biohemijskom i molekularnom nivou, međusobno su povezani, regulisani i usklađeni u integrisani sistem odgovora na promene u životnoj sredini. Mnogi ključni regulatorni i funkcionalni molekuli koji učestvuju u odgovoru na abiotički stres su identifikovani i time je otvorena mogućnost primene novih strategija u upravljanju stresom. Tema ovog rada su molekularni i biohemijski mehanizmi odgovora drvenastih vrsta biljaka na različite tipove abiotičkog stresa, sa posebnim osvrtom na topole., More frequent and more intense changes in environmental factors act as a stress on the plants. Faced with these changes, plants have developed mechanisms of tolerance and adaptation, which enable them to survive. These mechanisms operate on the morphological, physiological, biochemical and molecular level, are interconnected, regulated and coordinated in an integrated system response to changes in the environment. Many key regulatory and functional molecules involved in the response to abiotic stress are identified, thus opening the possibility for implementation of new strategies in the stress management. This paper outlines the molecular and biochemical mechanisms of response of woody plants to different types of abiotic stress, with special emphasis on poplars.",
publisher = "Univerzitet u Novom Sadu - Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad",
journal = "Topola",
title = "Molekularni mehanizmi odgovora drvenastih vrsta biljaka na abiotički stres, Molecular mechanisms of woody plant species abiotic stress response",
pages = "86-67",
number = "189-190",
url = "https://hdl.handle.net/21.15107/rcub_imagine_546"
}

Trudić, B., Radović, S., Galović, V., Jovanović, Ž.,& Stanisavljević, N.. (2012). Molekularni mehanizmi odgovora drvenastih vrsta biljaka na abiotički stres. in Topola
Univerzitet u Novom Sadu - Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad.(189-190), 67-86.
https://hdl.handle.net/21.15107/rcub_imagine_546

Trudić B, Radović S, Galović V, Jovanović Ž, Stanisavljević N. Molekularni mehanizmi odgovora drvenastih vrsta biljaka na abiotički stres. in Topola. 2012;(189-190):67-86.
https://hdl.handle.net/21.15107/rcub_imagine_546 .

Trudić, Branislav, Radović, Svetlana, Galović, Vladislava, Jovanović, Živko, Stanisavljević, Nemanja, "Molekularni mehanizmi odgovora drvenastih vrsta biljaka na abiotički stres" in Topola, no. 189-190 (2012):67-86,
https://hdl.handle.net/21.15107/rcub_imagine_546 .

TY  - JOUR
AU  - Medović, Aleksandar
AU  - Mikić, Aleksandar
AU  - Ćupina, Branko
AU  - Jovanović, Živko
AU  - Radović, Svetlana
AU  - Nikolić, Aleksandra
AU  - Stanisavljević, Nemanja
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/481
AB  - Arheološki lokalitet Hisar je gradinsko naselje Brnjičke kulturne grupe (gvozdeno doba I u Pomoravlju), podignuto na vrhu, odnosno na platou brda Hisar koje kontroliše zonu ušća Jablanice i Veternice u Južnu Moravu i najveći deo Leskovačke kotline. Prilikom arheoloških istraživanja 2005. godine u objektima koji se vezuju za Brnjica II a fazu (12. vek pre nove ere) pronađene su dve velike količine ugljenisanih semena graška (Pisum sativum L) i urova (Vicia ervilia (L) Willd). Zalihe ove dve zrnaste mahunarke bile su skoro čiste, sa malim procentom nečistoća koje su pre svega činili plodovi, pre svega žitarica, drugih mahunarki (sočivo - Lens culinaris Medik. i bob - Vicia faba L), ali i biljaka uljarica, što ukazuje da su grašak i urov bili odvojeno uzgajani, a potom i odvojeno skladišteni. Ovi nalazi, a pre svega nesvakidašnji nalaz zalihe graška, potvrđuju dugu tradiciju uzgajanja mahunarki u leskovačkom kraju.
AB  - A lucky find of 2,572 charred pea (Pisum sativum L) seeds in a single archaeobotanical sample from the hill fort settlement Hissar near Leskovac represents a unique example in Bronze / Iron Age research in South East Europe. Another mass storage of bitter vetch (Vicia ervilia (L) Willd) from the same site and period (Brnjica cultural group, beginning of the Iron Age in the Morava valley) confirms a long tradition of the city of Leskovac region as a pulse crop production centre in modern Serbia. Both pulse storages were almost pure with small amount of admixture from other crops mainly cereals, other pulses, such as lentil (Lens culinaris Medik) and broad bean (Vicia faba L), and oil/fibre plants. Both pulses were cultivated as main crops and were stored separately.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo prvi - dve zalihe ugljenisanih zrnastih mahunarki sa gradinskog naselja Hisar kod Leskovca, južno Pomoravlje
T1  - Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part one: Two charred pulse crop storages of the fortified hill fort settlement Hissar in Leskovac, South Serbia
EP  - 226
IS  - 1
SP  - 219
VL  - 48
DO  - 10.5937/ratpov1101219M
ER  - 

@article{
author = "Medović, Aleksandar and Mikić, Aleksandar and Ćupina, Branko and Jovanović, Živko and Radović, Svetlana and Nikolić, Aleksandra and Stanisavljević, Nemanja",
year = "2011",
abstract = "Arheološki lokalitet Hisar je gradinsko naselje Brnjičke kulturne grupe (gvozdeno doba I u Pomoravlju), podignuto na vrhu, odnosno na platou brda Hisar koje kontroliše zonu ušća Jablanice i Veternice u Južnu Moravu i najveći deo Leskovačke kotline. Prilikom arheoloških istraživanja 2005. godine u objektima koji se vezuju za Brnjica II a fazu (12. vek pre nove ere) pronađene su dve velike količine ugljenisanih semena graška (Pisum sativum L) i urova (Vicia ervilia (L) Willd). Zalihe ove dve zrnaste mahunarke bile su skoro čiste, sa malim procentom nečistoća koje su pre svega činili plodovi, pre svega žitarica, drugih mahunarki (sočivo - Lens culinaris Medik. i bob - Vicia faba L), ali i biljaka uljarica, što ukazuje da su grašak i urov bili odvojeno uzgajani, a potom i odvojeno skladišteni. Ovi nalazi, a pre svega nesvakidašnji nalaz zalihe graška, potvrđuju dugu tradiciju uzgajanja mahunarki u leskovačkom kraju., A lucky find of 2,572 charred pea (Pisum sativum L) seeds in a single archaeobotanical sample from the hill fort settlement Hissar near Leskovac represents a unique example in Bronze / Iron Age research in South East Europe. Another mass storage of bitter vetch (Vicia ervilia (L) Willd) from the same site and period (Brnjica cultural group, beginning of the Iron Age in the Morava valley) confirms a long tradition of the city of Leskovac region as a pulse crop production centre in modern Serbia. Both pulse storages were almost pure with small amount of admixture from other crops mainly cereals, other pulses, such as lentil (Lens culinaris Medik) and broad bean (Vicia faba L), and oil/fibre plants. Both pulses were cultivated as main crops and were stored separately.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo prvi - dve zalihe ugljenisanih zrnastih mahunarki sa gradinskog naselja Hisar kod Leskovca, južno Pomoravlje, Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part one: Two charred pulse crop storages of the fortified hill fort settlement Hissar in Leskovac, South Serbia",
pages = "226-219",
number = "1",
volume = "48",
doi = "10.5937/ratpov1101219M"
}

Medović, A., Mikić, A., Ćupina, B., Jovanović, Ž., Radović, S., Nikolić, A.,& Stanisavljević, N.. (2011). Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo prvi - dve zalihe ugljenisanih zrnastih mahunarki sa gradinskog naselja Hisar kod Leskovca, južno Pomoravlje. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 48(1), 219-226.
https://doi.org/10.5937/ratpov1101219M

Medović A, Mikić A, Ćupina B, Jovanović Ž, Radović S, Nikolić A, Stanisavljević N. Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo prvi - dve zalihe ugljenisanih zrnastih mahunarki sa gradinskog naselja Hisar kod Leskovca, južno Pomoravlje. in Ratarstvo i povrtarstvo. 2011;48(1):219-226.
doi:10.5937/ratpov1101219M .

Medović, Aleksandar, Mikić, Aleksandar, Ćupina, Branko, Jovanović, Živko, Radović, Svetlana, Nikolić, Aleksandra, Stanisavljević, Nemanja, "Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo prvi - dve zalihe ugljenisanih zrnastih mahunarki sa gradinskog naselja Hisar kod Leskovca, južno Pomoravlje" in Ratarstvo i povrtarstvo, 48, no. 1 (2011):219-226,
https://doi.org/10.5937/ratpov1101219M . .

TY  - JOUR
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Nikolić, Aleksandra
AU  - Medović, Aleksandar
AU  - Mikić, Aleksandar
AU  - Radović, Svetlana
AU  - Đorđević, Vuk
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/504
AB  - Primenom dve različite procedure ekstrahovana je drevna DNK iz ugljenisanih semena graška i urova starih 3200 godina sa lokaliteta Hisar kod Leskovca. Korišćena je modifikovana CTAB metoda i dobijena je relativno mala količina drevne DNK u poređenju sa drugom primenjenom metodom - komercijalno dostupnim kitom za izolaciju DNK. Nakon ekstrakcije primenjeno je umnožavanje celog genoma pomoću Phi29 DNK polimeraze. Umnožena DNK je korišćena za PCR reakciju primenom prajmera za 26S rDNK gen, koji je lociran u jedarnom genomu. Dobijen je fragment iste veličine kao i PCR fragment 26S rDNK savremenih srodnika graška i urova. Može se zaključiti da je moguće ekstrahovati drevnu DNK iz ugljenisanih semena graška i urova i koristiti je za dalje arheobotaničke analize na molekularnom nivou.
AB  - The extracts were prepared from the samples of 3,200-year-old charred pea and bitter vetch seeds from the site of Hissar near Leskovac, South Serbia, using two different DNA extraction procedures. We used CTAB method with some modification and obtained low quantity of ancient DNA in comparison with the second method used - commercial available kit. After the extraction, a whole genome amplification using Phi29 DNA polymerase was performed. The amplified DNAs were used for PCR reaction using primers for 26S rDNA gene, which is located on the nuclear genome. The single band corresponding to 26S rDNA fragment from modern relatives was obtained. We conclude that DNA from charred pea and vetch seed can be extracted and used for further archaeobotanical analysis at the molecular level.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo drugi - ekstrakcija drevne DNK iz ugljenisanih semena sa nalazišta Hisar u južnoj Srbiji
T1  - Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part two: Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia
EP  - 232
IS  - 1
SP  - 227
VL  - 48
DO  - 10.5937/ratpov1101227J
ER  - 

@article{
author = "Jovanović, Živko and Stanisavljević, Nemanja and Nikolić, Aleksandra and Medović, Aleksandar and Mikić, Aleksandar and Radović, Svetlana and Đorđević, Vuk",
year = "2011",
abstract = "Primenom dve različite procedure ekstrahovana je drevna DNK iz ugljenisanih semena graška i urova starih 3200 godina sa lokaliteta Hisar kod Leskovca. Korišćena je modifikovana CTAB metoda i dobijena je relativno mala količina drevne DNK u poređenju sa drugom primenjenom metodom - komercijalno dostupnim kitom za izolaciju DNK. Nakon ekstrakcije primenjeno je umnožavanje celog genoma pomoću Phi29 DNK polimeraze. Umnožena DNK je korišćena za PCR reakciju primenom prajmera za 26S rDNK gen, koji je lociran u jedarnom genomu. Dobijen je fragment iste veličine kao i PCR fragment 26S rDNK savremenih srodnika graška i urova. Može se zaključiti da je moguće ekstrahovati drevnu DNK iz ugljenisanih semena graška i urova i koristiti je za dalje arheobotaničke analize na molekularnom nivou., The extracts were prepared from the samples of 3,200-year-old charred pea and bitter vetch seeds from the site of Hissar near Leskovac, South Serbia, using two different DNA extraction procedures. We used CTAB method with some modification and obtained low quantity of ancient DNA in comparison with the second method used - commercial available kit. After the extraction, a whole genome amplification using Phi29 DNA polymerase was performed. The amplified DNAs were used for PCR reaction using primers for 26S rDNA gene, which is located on the nuclear genome. The single band corresponding to 26S rDNA fragment from modern relatives was obtained. We conclude that DNA from charred pea and vetch seed can be extracted and used for further archaeobotanical analysis at the molecular level.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo drugi - ekstrakcija drevne DNK iz ugljenisanih semena sa nalazišta Hisar u južnoj Srbiji, Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part two: Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia",
pages = "232-227",
number = "1",
volume = "48",
doi = "10.5937/ratpov1101227J"
}

Jovanović, Ž., Stanisavljević, N., Nikolić, A., Medović, A., Mikić, A., Radović, S.,& Đorđević, V.. (2011). Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo drugi - ekstrakcija drevne DNK iz ugljenisanih semena sa nalazišta Hisar u južnoj Srbiji. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 48(1), 227-232.
https://doi.org/10.5937/ratpov1101227J

Jovanović Ž, Stanisavljević N, Nikolić A, Medović A, Mikić A, Radović S, Đorđević V. Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo drugi - ekstrakcija drevne DNK iz ugljenisanih semena sa nalazišta Hisar u južnoj Srbiji. in Ratarstvo i povrtarstvo. 2011;48(1):227-232.
doi:10.5937/ratpov1101227J .

Jovanović, Živko, Stanisavljević, Nemanja, Nikolić, Aleksandra, Medović, Aleksandar, Mikić, Aleksandar, Radović, Svetlana, Đorđević, Vuk, "Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo drugi - ekstrakcija drevne DNK iz ugljenisanih semena sa nalazišta Hisar u južnoj Srbiji" in Ratarstvo i povrtarstvo, 48, no. 1 (2011):227-232,
https://doi.org/10.5937/ratpov1101227J . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Nikolić, Dragana
AU  - Jovanović, Živko
AU  - Samardžić, Jelena
AU  - Radović, Svetlana R.
AU  - Maksimović, Vesna R.
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/528
AB  - Oxidative stress and antioxidative defense system activity were studied in buckwheat leaves after complete submergence and re-aeration. The levels of H2O2 and lipid peroxidation were found to be significantly higher in stressed than in untreated buckwheat leaves. Enzymes catalyzing the degradation of H2O2 and peroxides were shown to participate actively, whereas superoxide dismutase did not take part in the buckwheat leaf response to flooding stress. The most prominent increase in antioxidative enzyme activities was noticed upon return to air, when the strongest oxidative stress occurred and the need for antioxidative defense was the greatest.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Antioxidative enzymes in the response of buckwheat (Fagopyrum esculentum Moench) to complete submergence
EP  - 405
IS  - 2
SP  - 399
VL  - 63
DO  - 10.2298/ABS1102399S
ER  - 

@article{
author = "Stanisavljević, Nemanja and Nikolić, Dragana and Jovanović, Živko and Samardžić, Jelena and Radović, Svetlana R. and Maksimović, Vesna R.",
year = "2011",
abstract = "Oxidative stress and antioxidative defense system activity were studied in buckwheat leaves after complete submergence and re-aeration. The levels of H2O2 and lipid peroxidation were found to be significantly higher in stressed than in untreated buckwheat leaves. Enzymes catalyzing the degradation of H2O2 and peroxides were shown to participate actively, whereas superoxide dismutase did not take part in the buckwheat leaf response to flooding stress. The most prominent increase in antioxidative enzyme activities was noticed upon return to air, when the strongest oxidative stress occurred and the need for antioxidative defense was the greatest.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Antioxidative enzymes in the response of buckwheat (Fagopyrum esculentum Moench) to complete submergence",
pages = "405-399",
number = "2",
volume = "63",
doi = "10.2298/ABS1102399S"
}

Stanisavljević, N., Nikolić, D., Jovanović, Ž., Samardžić, J., Radović, S. R.,& Maksimović, V. R.. (2011). Antioxidative enzymes in the response of buckwheat (Fagopyrum esculentum Moench) to complete submergence. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(2), 399-405.
https://doi.org/10.2298/ABS1102399S

Stanisavljević N, Nikolić D, Jovanović Ž, Samardžić J, Radović SR, Maksimović VR. Antioxidative enzymes in the response of buckwheat (Fagopyrum esculentum Moench) to complete submergence. in Archives of Biological Sciences. 2011;63(2):399-405.
doi:10.2298/ABS1102399S .

Stanisavljević, Nemanja, Nikolić, Dragana, Jovanović, Živko, Samardžić, Jelena, Radović, Svetlana R., Maksimović, Vesna R., "Antioxidative enzymes in the response of buckwheat (Fagopyrum esculentum Moench) to complete submergence" in Archives of Biological Sciences, 63, no. 2 (2011):399-405,
https://doi.org/10.2298/ABS1102399S . .

TY  - JOUR
AU  - Samardžić, Jelena
AU  - Nikolić, Dragana
AU  - Timotijević, Gordana
AU  - Jovanović, Živko
AU  - Milisavljević, Mira
AU  - Maksimović, Vesna R.
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/436
AB  - Metallothionein type 3 (MT3) expression has previously been detected in leaves fruits and developing somatic embryos in different plant species However specific tissular and cellular localization of MT3 transcripts have remained unidentified In this study in situ RNA-RNA analysis revealed buckwheat metallothionein type 3 (FeMT3) transcript localization in vascular elements mesophyll and guard cells of leaves vascular tissue of roots and throughout the whole embryo Changes in FeMT3 mRNA levels in response to drought and oxidative stress as well as ROS scavenging abilities of the FeMT3 protein in yeast were also detected indicating possible involvement of FeMT3 in stress defense and ROS related cellular processes
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Plant Physiology
T1  - Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum)
EP  - 1411
IS  - 16
SP  - 1407
VL  - 167
DO  - 10.1016/j.jplph.2010.05.016
ER  - 

@article{
author = "Samardžić, Jelena and Nikolić, Dragana and Timotijević, Gordana and Jovanović, Živko and Milisavljević, Mira and Maksimović, Vesna R.",
year = "2010",
abstract = "Metallothionein type 3 (MT3) expression has previously been detected in leaves fruits and developing somatic embryos in different plant species However specific tissular and cellular localization of MT3 transcripts have remained unidentified In this study in situ RNA-RNA analysis revealed buckwheat metallothionein type 3 (FeMT3) transcript localization in vascular elements mesophyll and guard cells of leaves vascular tissue of roots and throughout the whole embryo Changes in FeMT3 mRNA levels in response to drought and oxidative stress as well as ROS scavenging abilities of the FeMT3 protein in yeast were also detected indicating possible involvement of FeMT3 in stress defense and ROS related cellular processes",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Plant Physiology",
title = "Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum)",
pages = "1411-1407",
number = "16",
volume = "167",
doi = "10.1016/j.jplph.2010.05.016"
}

Samardžić, J., Nikolić, D., Timotijević, G., Jovanović, Ž., Milisavljević, M.,& Maksimović, V. R.. (2010). Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum). in Journal of Plant Physiology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 167(16), 1407-1411.
https://doi.org/10.1016/j.jplph.2010.05.016

Samardžić J, Nikolić D, Timotijević G, Jovanović Ž, Milisavljević M, Maksimović VR. Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum). in Journal of Plant Physiology. 2010;167(16):1407-1411.
doi:10.1016/j.jplph.2010.05.016 .

Samardžić, Jelena, Nikolić, Dragana, Timotijević, Gordana, Jovanović, Živko, Milisavljević, Mira, Maksimović, Vesna R., "Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum)" in Journal of Plant Physiology, 167, no. 16 (2010):1407-1411,
https://doi.org/10.1016/j.jplph.2010.05.016 . .

TY  - JOUR
AU  - Miljuš-Đukić, Jovanka
AU  - Banović Đeri, Bojana
AU  - Jovanović, Živko
AU  - Majić, Dragana
AU  - Milisavljević, Mira
AU  - Samardžić, Jelena
AU  - Timotijević, Gordana
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/468
AB  - Progress in biotechnolosy and genetic engineering enabled production of genetically modified (GM) plants, with novel characteristics due to bacterial or unrelated plants genes introduced into their genomes, like tolerance to herbicides and resistance to various diseases. Yields from GM crops Yearly rise around the world, but the public concerns oil using them, and subsequently derived food and feed products, forced European Commission to regulate the usage of GM organisms (GMOs) by the EU Directive 2001/18/EC. The EU regulations 1829/2003 and 1830/2003 require food industry to label all products containing more than 0.9% GM DNA To meet the regulative demands it is necessary to detect the presence of GM event in food/feed by PCR techniques or ELISA immunological testing. In Serbia, not all EU member, the GMO law exists at the state level. Controls include a border network of phytosanitary and veterinary inspections authorized to sample imported food/feed, institutions authorized to check file food/feed quality on Serbian markets and prior to its exporting and laboratories for testing GM DNA presence in samples. At the time analyses presented in this paper were performed only Monsanto's Roundup Ready soybean meal was approved for putting oil the market. During the 2006, 2007, and 2008 different samples of food/feed were collected among Serbian retail markets and tested to the soybean Roundup Ready presence. Analyses done using qualitative and quantitative PCR methods showed high percent of Roundup Ready samples among food/feed with similar values on yearly level, while quantitative amount ranged from 0 to 50%.
T2  - Romanian Biotechnological Letters
T1  - Abundance of soybean Roundup Ready modification in food and feed samples from Serbian retail markets
EP  - 109
IS  - SUPPL.1
SP  - 102
VL  - 15
UR  - https://hdl.handle.net/21.15107/rcub_imagine_468
ER  - 

@article{
author = "Miljuš-Đukić, Jovanka and Banović Đeri, Bojana and Jovanović, Živko and Majić, Dragana and Milisavljević, Mira and Samardžić, Jelena and Timotijević, Gordana",
year = "2010",
abstract = "Progress in biotechnolosy and genetic engineering enabled production of genetically modified (GM) plants, with novel characteristics due to bacterial or unrelated plants genes introduced into their genomes, like tolerance to herbicides and resistance to various diseases. Yields from GM crops Yearly rise around the world, but the public concerns oil using them, and subsequently derived food and feed products, forced European Commission to regulate the usage of GM organisms (GMOs) by the EU Directive 2001/18/EC. The EU regulations 1829/2003 and 1830/2003 require food industry to label all products containing more than 0.9% GM DNA To meet the regulative demands it is necessary to detect the presence of GM event in food/feed by PCR techniques or ELISA immunological testing. In Serbia, not all EU member, the GMO law exists at the state level. Controls include a border network of phytosanitary and veterinary inspections authorized to sample imported food/feed, institutions authorized to check file food/feed quality on Serbian markets and prior to its exporting and laboratories for testing GM DNA presence in samples. At the time analyses presented in this paper were performed only Monsanto's Roundup Ready soybean meal was approved for putting oil the market. During the 2006, 2007, and 2008 different samples of food/feed were collected among Serbian retail markets and tested to the soybean Roundup Ready presence. Analyses done using qualitative and quantitative PCR methods showed high percent of Roundup Ready samples among food/feed with similar values on yearly level, while quantitative amount ranged from 0 to 50%.",
journal = "Romanian Biotechnological Letters",
title = "Abundance of soybean Roundup Ready modification in food and feed samples from Serbian retail markets",
pages = "109-102",
number = "SUPPL.1",
volume = "15",
url = "https://hdl.handle.net/21.15107/rcub_imagine_468"
}

Miljuš-Đukić, J., Banović Đeri, B., Jovanović, Ž., Majić, D., Milisavljević, M., Samardžić, J.,& Timotijević, G.. (2010). Abundance of soybean Roundup Ready modification in food and feed samples from Serbian retail markets. in Romanian Biotechnological Letters, 15(SUPPL.1), 102-109.
https://hdl.handle.net/21.15107/rcub_imagine_468

Miljuš-Đukić J, Banović Đeri B, Jovanović Ž, Majić D, Milisavljević M, Samardžić J, Timotijević G. Abundance of soybean Roundup Ready modification in food and feed samples from Serbian retail markets. in Romanian Biotechnological Letters. 2010;15(SUPPL.1):102-109.
https://hdl.handle.net/21.15107/rcub_imagine_468 .

Miljuš-Đukić, Jovanka, Banović Đeri, Bojana, Jovanović, Živko, Majić, Dragana, Milisavljević, Mira, Samardžić, Jelena, Timotijević, Gordana, "Abundance of soybean Roundup Ready modification in food and feed samples from Serbian retail markets" in Romanian Biotechnological Letters, 15, no. SUPPL.1 (2010):102-109,
https://hdl.handle.net/21.15107/rcub_imagine_468 .