TY  - JOUR
AU  - Taxeidis, George
AU  - Nikolaivits, Efstratios
AU  - Siaperas, Romanos
AU  - Gkountela, Christina
AU  - Vouyiouka, Stamatina
AU  - Pantelić, Brana
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0269749123004621
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1797
AB  - The uncontrollable disposal of plastic waste has raised the concern of the scientific community, which tries to face this environmental burden by discovering and applying new techniques. Regarding the biotechnology field, several important microorganisms possessing the necessary enzymatic arsenal to utilize recalcitrant synthetic polymers as an energy source have been discovered. In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based polyurethane (PU) and low-density polyethylene (LDPE). For this, ImpranIil® DLN-SD and a mixture of long-chain alkanes were used as sole carbon sources, indicating not only the most promising strains in agar plate screening but also inducing the secretion of depolymerizing enzymatic activities, useful for polymer degradation. The agar plate screening revealed three fungal strains belonging to Fusarium and Aspergillus genera, whose secretome was further studied for its ability to degrade the aforementioned non-treated polymers. Specifically for ether-based PU, the secretome of a Fusarium species reduced the sample mass and the average molecular weight of the polymer by 24.5 and 20.4%, respectively, while the secretome of an Aspergillus species caused changes in the molecular structure of LDPE, as evidenced by FTIR. The proteomics analysis revealed that the enzymatic activities induced in presence of Impranil® DLN-SD can be associated with urethane bond cleavage, a fact which was also supported by the observed degradation of the ether-based PU. Although, the mechanism of LDPE degradation was not completely elucidated, the presence of oxidative enzymes could be the main factor contributing to polymer modification.
T2  - Environmental Pollution
T1  - Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes
SP  - 121460
VL  - 325
DO  - 10.1016/j.envpol.2023.121460
ER  - 

@article{
author = "Taxeidis, George and Nikolaivits, Efstratios and Siaperas, Romanos and Gkountela, Christina and Vouyiouka, Stamatina and Pantelić, Brana and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
year = "2023",
abstract = "The uncontrollable disposal of plastic waste has raised the concern of the scientific community, which tries to face this environmental burden by discovering and applying new techniques. Regarding the biotechnology field, several important microorganisms possessing the necessary enzymatic arsenal to utilize recalcitrant synthetic polymers as an energy source have been discovered. In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based polyurethane (PU) and low-density polyethylene (LDPE). For this, ImpranIil® DLN-SD and a mixture of long-chain alkanes were used as sole carbon sources, indicating not only the most promising strains in agar plate screening but also inducing the secretion of depolymerizing enzymatic activities, useful for polymer degradation. The agar plate screening revealed three fungal strains belonging to Fusarium and Aspergillus genera, whose secretome was further studied for its ability to degrade the aforementioned non-treated polymers. Specifically for ether-based PU, the secretome of a Fusarium species reduced the sample mass and the average molecular weight of the polymer by 24.5 and 20.4%, respectively, while the secretome of an Aspergillus species caused changes in the molecular structure of LDPE, as evidenced by FTIR. The proteomics analysis revealed that the enzymatic activities induced in presence of Impranil® DLN-SD can be associated with urethane bond cleavage, a fact which was also supported by the observed degradation of the ether-based PU. Although, the mechanism of LDPE degradation was not completely elucidated, the presence of oxidative enzymes could be the main factor contributing to polymer modification.",
journal = "Environmental Pollution",
title = "Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes",
pages = "121460",
volume = "325",
doi = "10.1016/j.envpol.2023.121460"
}

Taxeidis, G., Nikolaivits, E., Siaperas, R., Gkountela, C., Vouyiouka, S., Pantelić, B., Nikodinović-Runić, J.,& Topakas, E.. (2023). Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes. in Environmental Pollution, 325, 121460.
https://doi.org/10.1016/j.envpol.2023.121460

Taxeidis G, Nikolaivits E, Siaperas R, Gkountela C, Vouyiouka S, Pantelić B, Nikodinović-Runić J, Topakas E. Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes. in Environmental Pollution. 2023;325:121460.
doi:10.1016/j.envpol.2023.121460 .

Taxeidis, George, Nikolaivits, Efstratios, Siaperas, Romanos, Gkountela, Christina, Vouyiouka, Stamatina, Pantelić, Brana, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes" in Environmental Pollution, 325 (2023):121460,
https://doi.org/10.1016/j.envpol.2023.121460 . .

TY  - JOUR
AU  - Taxeidis, George
AU  - Nikolaivits, Efstratios
AU  - Siaperas, Romanos
AU  - Gkountela, Christina
AU  - Vouyiouka, Stamatina
AU  - Pantelić, Brana
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0269749123004621
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1800
AB  - The uncontrollable disposal of plastic waste has raised the concern of the scientific community, which tries to face this environmental burden by discovering and applying new techniques. Regarding the biotechnology field, several important microorganisms possessing the necessary enzymatic arsenal to utilize recalcitrant synthetic polymers as an energy source have been discovered. In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based polyurethane (PU) and low-density polyethylene (LDPE). For this, ImpranIil® DLN-SD and a mixture of long-chain alkanes were used as sole carbon sources, indicating not only the most promising strains in agar plate screening but also inducing the secretion of depolymerizing enzymatic activities, useful for polymer degradation. The agar plate screening revealed three fungal strains belonging to Fusarium and Aspergillus genera, whose secretome was further studied for its ability to degrade the aforementioned non-treated polymers. Specifically for ether-based PU, the secretome of a Fusarium species reduced the sample mass and the average molecular weight of the polymer by 24.5 and 20.4%, respectively, while the secretome of an Aspergillus species caused changes in the molecular structure of LDPE, as evidenced by FTIR. The proteomics analysis revealed that the enzymatic activities induced in presence of Impranil® DLN-SD can be associated with urethane bond cleavage, a fact which was also supported by the observed degradation of the ether-based PU. Although, the mechanism of LDPE degradation was not completely elucidated, the presence of oxidative enzymes could be the main factor contributing to polymer modification.
T2  - Environmental Pollution
T1  - Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes
SP  - 121460
VL  - 325
DO  - 10.1016/j.envpol.2023.121460
ER  - 

@article{
author = "Taxeidis, George and Nikolaivits, Efstratios and Siaperas, Romanos and Gkountela, Christina and Vouyiouka, Stamatina and Pantelić, Brana and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
year = "2023",
abstract = "The uncontrollable disposal of plastic waste has raised the concern of the scientific community, which tries to face this environmental burden by discovering and applying new techniques. Regarding the biotechnology field, several important microorganisms possessing the necessary enzymatic arsenal to utilize recalcitrant synthetic polymers as an energy source have been discovered. In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based polyurethane (PU) and low-density polyethylene (LDPE). For this, ImpranIil® DLN-SD and a mixture of long-chain alkanes were used as sole carbon sources, indicating not only the most promising strains in agar plate screening but also inducing the secretion of depolymerizing enzymatic activities, useful for polymer degradation. The agar plate screening revealed three fungal strains belonging to Fusarium and Aspergillus genera, whose secretome was further studied for its ability to degrade the aforementioned non-treated polymers. Specifically for ether-based PU, the secretome of a Fusarium species reduced the sample mass and the average molecular weight of the polymer by 24.5 and 20.4%, respectively, while the secretome of an Aspergillus species caused changes in the molecular structure of LDPE, as evidenced by FTIR. The proteomics analysis revealed that the enzymatic activities induced in presence of Impranil® DLN-SD can be associated with urethane bond cleavage, a fact which was also supported by the observed degradation of the ether-based PU. Although, the mechanism of LDPE degradation was not completely elucidated, the presence of oxidative enzymes could be the main factor contributing to polymer modification.",
journal = "Environmental Pollution",
title = "Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes",
pages = "121460",
volume = "325",
doi = "10.1016/j.envpol.2023.121460"
}

Taxeidis, G., Nikolaivits, E., Siaperas, R., Gkountela, C., Vouyiouka, S., Pantelić, B., Nikodinović-Runić, J.,& Topakas, E.. (2023). Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes. in Environmental Pollution, 325, 121460.
https://doi.org/10.1016/j.envpol.2023.121460

Taxeidis G, Nikolaivits E, Siaperas R, Gkountela C, Vouyiouka S, Pantelić B, Nikodinović-Runić J, Topakas E. Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes. in Environmental Pollution. 2023;325:121460.
doi:10.1016/j.envpol.2023.121460 .

Taxeidis, George, Nikolaivits, Efstratios, Siaperas, Romanos, Gkountela, Christina, Vouyiouka, Stamatina, Pantelić, Brana, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes" in Environmental Pollution, 325 (2023):121460,
https://doi.org/10.1016/j.envpol.2023.121460 . .

TY  - JOUR
AU  - Nikolaivits, Efstratios
AU  - Taxeidis, George
AU  - Gkountela, Christina
AU  - Vouyiouka, Stamatina
AU  - Maslak, Veselin
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1630
AB  - The uncontrolled release of plastics in the environment has rendered them ubiquitous around the planet, threatening the wildlife and human health. Biodegradation and valorization of plastics has emerged as an ecofriendly alternative to conventional management techniques. Discovery of novel polymer-degrading enzymes with diversified properties is hence an important task in order to explore different operational conditions for plastic-waste upcycling. In the present study, a barely studied psychrophilic enzyme (MoPE) from the Antractic bacterium Moraxella sp. was heterologously expressed, characterized and its potential in polymer degradation was further investigated. Based on its amino acid composition and structure, MoPE resembled PET-degrading enzymes, sharing features from both mesophilic and thermophilic homologues. MoPE hydrolyzes nonbiodegradable plastics, such as polyethylene terephthalate and polyurethane, as well as biodegradable
PB  - Elsevier, Amsterdam
T2  - Journal of Hazardous Materials
T1  - A polyesterase from the Antarctic bacterium Moraxella sp. degrades highly crystalline synthetic polymers
VL  - 434
DO  - 10.1016/j.jhazmat.2022.128900
ER  - 

@article{
author = "Nikolaivits, Efstratios and Taxeidis, George and Gkountela, Christina and Vouyiouka, Stamatina and Maslak, Veselin and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
year = "2022",
abstract = "The uncontrolled release of plastics in the environment has rendered them ubiquitous around the planet, threatening the wildlife and human health. Biodegradation and valorization of plastics has emerged as an ecofriendly alternative to conventional management techniques. Discovery of novel polymer-degrading enzymes with diversified properties is hence an important task in order to explore different operational conditions for plastic-waste upcycling. In the present study, a barely studied psychrophilic enzyme (MoPE) from the Antractic bacterium Moraxella sp. was heterologously expressed, characterized and its potential in polymer degradation was further investigated. Based on its amino acid composition and structure, MoPE resembled PET-degrading enzymes, sharing features from both mesophilic and thermophilic homologues. MoPE hydrolyzes nonbiodegradable plastics, such as polyethylene terephthalate and polyurethane, as well as biodegradable",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Hazardous Materials",
title = "A polyesterase from the Antarctic bacterium Moraxella sp. degrades highly crystalline synthetic polymers",
volume = "434",
doi = "10.1016/j.jhazmat.2022.128900"
}

Nikolaivits, E., Taxeidis, G., Gkountela, C., Vouyiouka, S., Maslak, V., Nikodinović-Runić, J.,& Topakas, E.. (2022). A polyesterase from the Antarctic bacterium Moraxella sp. degrades highly crystalline synthetic polymers. in Journal of Hazardous Materials
Elsevier, Amsterdam., 434.
https://doi.org/10.1016/j.jhazmat.2022.128900

Nikolaivits E, Taxeidis G, Gkountela C, Vouyiouka S, Maslak V, Nikodinović-Runić J, Topakas E. A polyesterase from the Antarctic bacterium Moraxella sp. degrades highly crystalline synthetic polymers. in Journal of Hazardous Materials. 2022;434.
doi:10.1016/j.jhazmat.2022.128900 .

Nikolaivits, Efstratios, Taxeidis, George, Gkountela, Christina, Vouyiouka, Stamatina, Maslak, Veselin, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "A polyesterase from the Antarctic bacterium Moraxella sp. degrades highly crystalline synthetic polymers" in Journal of Hazardous Materials, 434 (2022),
https://doi.org/10.1016/j.jhazmat.2022.128900 . .

TY  - JOUR
AU  - Nikolaivits, Efstratios
AU  - Taxeidis, George
AU  - Gkountela, Christina
AU  - Vouyiouka, Stamatina
AU  - Maslak, Veselin
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1564
AB  - The uncontrolled release of plastics in the environment has rendered them ubiquitous around the planet, threatening the wildlife and human health. Biodegradation and valorization of plastics has emerged as an ecofriendly alternative to conventional management techniques. Discovery of novel polymer-degrading enzymes with diversified properties is hence an important task in order to explore different operational conditions for plastic-waste upcycling. In the present study, a barely studied psychrophilic enzyme (MoPE) from the Antractic bacterium Moraxella sp. was heterologously expressed, characterized and its potential in polymer degradation was further investigated. Based on its amino acid composition and structure, MoPE resembled PET-degrading enzymes, sharing features from both mesophilic and thermophilic homologues. MoPE hydrolyzes nonbiodegradable plastics, such as polyethylene terephthalate and polyurethane, as well as biodegradable
PB  - Elsevier, Amsterdam
T2  - Journal of Hazardous Materials
T1  - A polyesterase from the Antarctic bacterium Moraxella sp. degrades highly crystalline synthetic polymers
VL  - 434
DO  - 10.1016/j.jhazmat.2022.128900
ER  - 

@article{
author = "Nikolaivits, Efstratios and Taxeidis, George and Gkountela, Christina and Vouyiouka, Stamatina and Maslak, Veselin and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
year = "2022",
abstract = "The uncontrolled release of plastics in the environment has rendered them ubiquitous around the planet, threatening the wildlife and human health. Biodegradation and valorization of plastics has emerged as an ecofriendly alternative to conventional management techniques. Discovery of novel polymer-degrading enzymes with diversified properties is hence an important task in order to explore different operational conditions for plastic-waste upcycling. In the present study, a barely studied psychrophilic enzyme (MoPE) from the Antractic bacterium Moraxella sp. was heterologously expressed, characterized and its potential in polymer degradation was further investigated. Based on its amino acid composition and structure, MoPE resembled PET-degrading enzymes, sharing features from both mesophilic and thermophilic homologues. MoPE hydrolyzes nonbiodegradable plastics, such as polyethylene terephthalate and polyurethane, as well as biodegradable",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Hazardous Materials",
title = "A polyesterase from the Antarctic bacterium Moraxella sp. degrades highly crystalline synthetic polymers",
volume = "434",
doi = "10.1016/j.jhazmat.2022.128900"
}

Nikolaivits, E., Taxeidis, G., Gkountela, C., Vouyiouka, S., Maslak, V., Nikodinović-Runić, J.,& Topakas, E.. (2022). A polyesterase from the Antarctic bacterium Moraxella sp. degrades highly crystalline synthetic polymers. in Journal of Hazardous Materials
Elsevier, Amsterdam., 434.
https://doi.org/10.1016/j.jhazmat.2022.128900

Nikolaivits E, Taxeidis G, Gkountela C, Vouyiouka S, Maslak V, Nikodinović-Runić J, Topakas E. A polyesterase from the Antarctic bacterium Moraxella sp. degrades highly crystalline synthetic polymers. in Journal of Hazardous Materials. 2022;434.
doi:10.1016/j.jhazmat.2022.128900 .

Nikolaivits, Efstratios, Taxeidis, George, Gkountela, Christina, Vouyiouka, Stamatina, Maslak, Veselin, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "A polyesterase from the Antarctic bacterium Moraxella sp. degrades highly crystalline synthetic polymers" in Journal of Hazardous Materials, 434 (2022),
https://doi.org/10.1016/j.jhazmat.2022.128900 . .