TY  - CONF
AU  - Sourkouni, G.
AU  - Kalogirou, Ch.
AU  - Papadimitriou, N.
AU  - Nenadović, Marija
AU  - Ponjavić, Marijana
AU  - Argirusis, N.
AU  - Pandis, P.
AU  - Rajasekaran, D.
AU  - Padamati, R.
AU  - Ferraro, A.
AU  - Nikodinović-Runić, Jasmina
AU  - Argirusis, Chr.
PY  - 2023
UR  - http://chania2023.uest.gr/poster.html
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2024
AB  - Plastics are artificial synthetic organic polymers that have been used in every area of daily life. However, because of their slow degradation rate, their use
is contentious. The treatment of the surface of the sample is considered necessary as enzymatic or bacterial attach is not possible, if the plastic surface
environment is not ideal. The main topic of this work is the investigation of the effect of atmospheric dielectric barrier discharge (DBD) plasma on the near
surface structure of polylactic acid (PLA) samples, which, in turn, can promote the adhesion of enzymes or bacteria for further biodegradation. In general,
plasma processes can already be considered as inherently environmental technologies.
Plasma processes enable resource saving through high energy utilization efficiency and thus, are environ-mentally friendly technologies. Atmospheric
pressure discharges (APDs) are useful because of their specific advantages over low-pressure ones. They do not need expensive vacuum equipment, and
generate nonthermal plasmas, which are more suitable for assembly line processes. Hence, this category of discharges has significant industrial
applications. The use of a dielectric barrier in the discharge gap helps prevent spark formation. DBDs exhibit two major discharge modes: filamentary and
glow (homogeneous). The glow discharge mode has obvious advantages over the filamentary one for applications such as treatment of surfaces and
deposition of thin films. Glow mode discharges with average power densities comparable to those of filamentary discharges are of enormous interest for
applications in which reliable control is required.
Here we will present the increased adhesion of bacteria strains on DBD plasma treated PLA foils which can lead to a better degradation of the PLA. X-ray
photoelectron spectroscopy (XPS) measurements of the foils prior to and after the treatment proved the changes on the polymer surface. A short
discussion of the possibilities the treatment opens is given.
C3  - 10th International Conference on Sustainable Solid Waste Management Chania
T1  - Plasma assisted bio-degradation  of poly-lactic acid (PLA)
VL  - 10
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2024
ER  - 

@conference{
author = "Sourkouni, G. and Kalogirou, Ch. and Papadimitriou, N. and Nenadović, Marija and Ponjavić, Marijana and Argirusis, N. and Pandis, P. and Rajasekaran, D. and Padamati, R. and Ferraro, A. and Nikodinović-Runić, Jasmina and Argirusis, Chr.",
year = "2023",
abstract = "Plastics are artificial synthetic organic polymers that have been used in every area of daily life. However, because of their slow degradation rate, their use
is contentious. The treatment of the surface of the sample is considered necessary as enzymatic or bacterial attach is not possible, if the plastic surface
environment is not ideal. The main topic of this work is the investigation of the effect of atmospheric dielectric barrier discharge (DBD) plasma on the near
surface structure of polylactic acid (PLA) samples, which, in turn, can promote the adhesion of enzymes or bacteria for further biodegradation. In general,
plasma processes can already be considered as inherently environmental technologies.
Plasma processes enable resource saving through high energy utilization efficiency and thus, are environ-mentally friendly technologies. Atmospheric
pressure discharges (APDs) are useful because of their specific advantages over low-pressure ones. They do not need expensive vacuum equipment, and
generate nonthermal plasmas, which are more suitable for assembly line processes. Hence, this category of discharges has significant industrial
applications. The use of a dielectric barrier in the discharge gap helps prevent spark formation. DBDs exhibit two major discharge modes: filamentary and
glow (homogeneous). The glow discharge mode has obvious advantages over the filamentary one for applications such as treatment of surfaces and
deposition of thin films. Glow mode discharges with average power densities comparable to those of filamentary discharges are of enormous interest for
applications in which reliable control is required.
Here we will present the increased adhesion of bacteria strains on DBD plasma treated PLA foils which can lead to a better degradation of the PLA. X-ray
photoelectron spectroscopy (XPS) measurements of the foils prior to and after the treatment proved the changes on the polymer surface. A short
discussion of the possibilities the treatment opens is given.",
journal = "10th International Conference on Sustainable Solid Waste Management Chania",
title = "Plasma assisted bio-degradation  of poly-lactic acid (PLA)",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2024"
}

Sourkouni, G., Kalogirou, Ch., Papadimitriou, N., Nenadović, M., Ponjavić, M., Argirusis, N., Pandis, P., Rajasekaran, D., Padamati, R., Ferraro, A., Nikodinović-Runić, J.,& Argirusis, Chr.. (2023). Plasma assisted bio-degradation  of poly-lactic acid (PLA). in 10th International Conference on Sustainable Solid Waste Management Chania, 10.
https://hdl.handle.net/21.15107/rcub_imagine_2024

Sourkouni G, Kalogirou C, Papadimitriou N, Nenadović M, Ponjavić M, Argirusis N, Pandis P, Rajasekaran D, Padamati R, Ferraro A, Nikodinović-Runić J, Argirusis C. Plasma assisted bio-degradation  of poly-lactic acid (PLA). in 10th International Conference on Sustainable Solid Waste Management Chania. 2023;10.
https://hdl.handle.net/21.15107/rcub_imagine_2024 .

Sourkouni, G., Kalogirou, Ch., Papadimitriou, N., Nenadović, Marija, Ponjavić, Marijana, Argirusis, N., Pandis, P., Rajasekaran, D., Padamati, R., Ferraro, A., Nikodinović-Runić, Jasmina, Argirusis, Chr., "Plasma assisted bio-degradation  of poly-lactic acid (PLA)" in 10th International Conference on Sustainable Solid Waste Management Chania, 10 (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2024 .

TY  - CONF
AU  - Milovanović, Jelena
AU  - Nenadović, Marija
AU  - Pantelić, Brana
AU  - Ponjavić,  Marijana
AU  - Sourkouni, Georgia
AU  - Kalogirou, Charalampia
AU  - Argirusis, Christos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://esabweb.org/E_CONGRESS/Poster+Programme/Day/Online/All+day/Enhanced+enzymatic+depolymerization+of+polylactic+acid+%28PLA%29+through+plasma+pretreatment+and+subsequent+conversion+to+biopolymer.html
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2232
AB  - Polylactic acid (PLA) serves as a bio-based alternative to fossil-based single-use plastics, biodegrading at high temperatures (58°C) and humidity during industrial composting. Despite enzymes' ability to catalyze reactions at near-ambient temperatures, polymer rigidity can impede efficient depolymerization. To address these challenges, we conducted a study of enzymatic PLA degradation at 42°C combined with green plasma pretreatment to help disrupt the crystalline regions within the polymer. Here we report the effect of length of plasma pretreatment on the rate of PLA degradation by enzyme mix containing commercial enzymes with reported PLA degrading activity. Results indicate that a 5-minute plasma pretreatment significantly enhances enzymatic degradation, with a 16% weight loss achieved in 4 weeks—a two-fold increase compared to untreated PLA. Furthermore, we report the valorization of PLA into bacterial nanocellulose after enzymatic hydrolysis of the samples.
PB  - European Society of Applied Biocatalysis
C3  - ESAB E-Congress
T1  - Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2232
ER  - 

@conference{
author = "Milovanović, Jelena and Nenadović, Marija and Pantelić, Brana and Ponjavić,  Marijana and Sourkouni, Georgia and Kalogirou, Charalampia and Argirusis, Christos and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Polylactic acid (PLA) serves as a bio-based alternative to fossil-based single-use plastics, biodegrading at high temperatures (58°C) and humidity during industrial composting. Despite enzymes' ability to catalyze reactions at near-ambient temperatures, polymer rigidity can impede efficient depolymerization. To address these challenges, we conducted a study of enzymatic PLA degradation at 42°C combined with green plasma pretreatment to help disrupt the crystalline regions within the polymer. Here we report the effect of length of plasma pretreatment on the rate of PLA degradation by enzyme mix containing commercial enzymes with reported PLA degrading activity. Results indicate that a 5-minute plasma pretreatment significantly enhances enzymatic degradation, with a 16% weight loss achieved in 4 weeks—a two-fold increase compared to untreated PLA. Furthermore, we report the valorization of PLA into bacterial nanocellulose after enzymatic hydrolysis of the samples.",
publisher = "European Society of Applied Biocatalysis",
journal = "ESAB E-Congress",
title = "Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2232"
}

Milovanović, J., Nenadović, M., Pantelić, B., Ponjavić, M., Sourkouni, G., Kalogirou, C., Argirusis, C.,& Nikodinović-Runić, J.. (2023). Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer. in ESAB E-Congress
European Society of Applied Biocatalysis..
https://hdl.handle.net/21.15107/rcub_imagine_2232

Milovanović J, Nenadović M, Pantelić B, Ponjavić M, Sourkouni G, Kalogirou C, Argirusis C, Nikodinović-Runić J. Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer. in ESAB E-Congress. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2232 .

Milovanović, Jelena, Nenadović, Marija, Pantelić, Brana, Ponjavić,  Marijana, Sourkouni, Georgia, Kalogirou, Charalampia, Argirusis, Christos, Nikodinović-Runić, Jasmina, "Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer" in ESAB E-Congress (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2232 .

TY  - CONF
AU  - Nenadović, Marija
AU  - Pantelić, Brana
AU  - Lazić, Jelena
AU  - Maslak, Veselin
AU  - Nikodinović-Runić, Jasmina
AU  - Milovanović, Jelena
PY  - 2023
UR  - https://afea.eventsair.com/10th-conference-of-mikrobiokosmos/abstract-book
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2237
AB  - PHAs are naturally made microbial polyesters that
have been commercialized as biodegradable
plastics. However, it has been shown that these
materials are not so easily biodegraded in natural
environments [1]. PHA depolymerases are key PHA
degrading enzymes and their identification and
characterization is of great interest and importance.
Currently, screening is done on polymeric
substrates using techniques such as clear zone
assays on agar or weight loss measurements.
Results obtained using these different methods
cannot be directly compared, since they depend
highly on the polymer used, PHA granules
preparation and assay conditions [2].
In order to design a more specific test for the
determination of PHA depolymerase activity, we
synthesized 3-hyoxyalkanoate monomers (3-HA
monomer) and 3-hyoxyalkanoic acid dimers (3-HA
dimer) and their respective p-nitrophenyl esters,
allowing for spectrophotometric determination of
their activity [3]. Compounds were characterized
using N and FTIR. Para-nitrophenyl labeled
substrates were then used in the enzymatic activity
assay with the benchmark polyhyoxyoctanoate
(PHO) depolymerase from Pseudomonas
fluorescens GK13 expressed in Escherichia coli
CodonPlus-RIPL hosts. This activity was compared
to recombinantly expressed leaf-branch compost
cutinase (LCC cutinase) and
polyethyleneterephtalate (PET) hyolyzing esterase
from Ideonella sakaiensis (IsPETase). Our initial
results revealed increased specificity of PHO
depolymerase towards newly synthetized
substrates, suggesting their suitability for specific
screens and isolation of new mcl-PHA
depolymerases, as well as in high throughput
screening assays designed for guiding their
directed evolution.
C3  - 10th Conference of Mikrobiokosmos
T1  - Medium chain length polyhyoxyalkanoates (mcl-PHA) model compounds for the discovery of novel PHA depolymerases
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2237
ER  - 

@conference{
author = "Nenadović, Marija and Pantelić, Brana and Lazić, Jelena and Maslak, Veselin and Nikodinović-Runić, Jasmina and Milovanović, Jelena",
year = "2023",
abstract = "PHAs are naturally made microbial polyesters that
have been commercialized as biodegradable
plastics. However, it has been shown that these
materials are not so easily biodegraded in natural
environments [1]. PHA depolymerases are key PHA
degrading enzymes and their identification and
characterization is of great interest and importance.
Currently, screening is done on polymeric
substrates using techniques such as clear zone
assays on agar or weight loss measurements.
Results obtained using these different methods
cannot be directly compared, since they depend
highly on the polymer used, PHA granules
preparation and assay conditions [2].
In order to design a more specific test for the
determination of PHA depolymerase activity, we
synthesized 3-hyoxyalkanoate monomers (3-HA
monomer) and 3-hyoxyalkanoic acid dimers (3-HA
dimer) and their respective p-nitrophenyl esters,
allowing for spectrophotometric determination of
their activity [3]. Compounds were characterized
using N and FTIR. Para-nitrophenyl labeled
substrates were then used in the enzymatic activity
assay with the benchmark polyhyoxyoctanoate
(PHO) depolymerase from Pseudomonas
fluorescens GK13 expressed in Escherichia coli
CodonPlus-RIPL hosts. This activity was compared
to recombinantly expressed leaf-branch compost
cutinase (LCC cutinase) and
polyethyleneterephtalate (PET) hyolyzing esterase
from Ideonella sakaiensis (IsPETase). Our initial
results revealed increased specificity of PHO
depolymerase towards newly synthetized
substrates, suggesting their suitability for specific
screens and isolation of new mcl-PHA
depolymerases, as well as in high throughput
screening assays designed for guiding their
directed evolution.",
journal = "10th Conference of Mikrobiokosmos",
title = "Medium chain length polyhyoxyalkanoates (mcl-PHA) model compounds for the discovery of novel PHA depolymerases",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2237"
}

Nenadović, M., Pantelić, B., Lazić, J., Maslak, V., Nikodinović-Runić, J.,& Milovanović, J.. (2023). Medium chain length polyhyoxyalkanoates (mcl-PHA) model compounds for the discovery of novel PHA depolymerases. in 10th Conference of Mikrobiokosmos.
https://hdl.handle.net/21.15107/rcub_imagine_2237

Nenadović M, Pantelić B, Lazić J, Maslak V, Nikodinović-Runić J, Milovanović J. Medium chain length polyhyoxyalkanoates (mcl-PHA) model compounds for the discovery of novel PHA depolymerases. in 10th Conference of Mikrobiokosmos. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2237 .

Nenadović, Marija, Pantelić, Brana, Lazić, Jelena, Maslak, Veselin, Nikodinović-Runić, Jasmina, Milovanović, Jelena, "Medium chain length polyhyoxyalkanoates (mcl-PHA) model compounds for the discovery of novel PHA depolymerases" in 10th Conference of Mikrobiokosmos (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2237 .

TY  - JOUR
AU  - Ašanin, Darko
AU  - Andrejević, Tina
AU  - Nenadović, Marija
AU  - Rodić, Marko
AU  - Vojnović, Sandra
AU  - Djuran, Miloš
AU  - Glišić, Biljana
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0277538723003078
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2067
AB  - In the present study, synthesis of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine (1,6-naph), {[Ag(1,6-naph)(H2O)](BF4)}n (1) and [AuCl3(1,6-naph)] (2), was reported. The methods used for the structural characterization of a new compound 1 included IR, NMR (1H and 13C) and UV-Vis spectroscopy, cyclic voltammetry and single-crystal X-ray diffraction analysis. The crystallographic results showed that compound 1 represents silver(I) coordination polymer, in which 1,6-naph ligand acts as a bidentate bridging ligand connecting two Ag(I) ions via its N1 and N6 nitrogen atoms, while the third coordination site of the metal ion is occupied by the water oxygen atom, resulted in a T-shape geometry. Compounds 1 and 2 were evaluated in vitro for antimicrobial activity against five bacterial and two Candida species, while their cytotoxicity was tested on the normal human lung fibroblast cell line (MRC-5). Compound 1 has manifested a remarkable antifungal activity on both tested Candida strains (C. albicans and C. parapsilosis) with minimal inhibitory concentrations (MICs) of 1.43 and 11.38 µM (0.49 and 3.9 µg/mL), respectively, while no significant antimicrobial activity was observed for 2. Moreover, silver(I) coordination polymer 1 inhibits the hyphae formation of C. albicans at subinhibitory concentration. The binding affinity of both compounds 1 and 2 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy, indicating their ability to interact with these biomolecules, with compound 2 being more reactive.
T2  - Polyhedron
T1  - Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine
IS  - 1
SP  - 116585
VL  - 244
DO  - 10.1016/j.poly.2023.116585
ER  - 

@article{
author = "Ašanin, Darko and Andrejević, Tina and Nenadović, Marija and Rodić, Marko and Vojnović, Sandra and Djuran, Miloš and Glišić, Biljana",
year = "2023",
abstract = "In the present study, synthesis of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine (1,6-naph), {[Ag(1,6-naph)(H2O)](BF4)}n (1) and [AuCl3(1,6-naph)] (2), was reported. The methods used for the structural characterization of a new compound 1 included IR, NMR (1H and 13C) and UV-Vis spectroscopy, cyclic voltammetry and single-crystal X-ray diffraction analysis. The crystallographic results showed that compound 1 represents silver(I) coordination polymer, in which 1,6-naph ligand acts as a bidentate bridging ligand connecting two Ag(I) ions via its N1 and N6 nitrogen atoms, while the third coordination site of the metal ion is occupied by the water oxygen atom, resulted in a T-shape geometry. Compounds 1 and 2 were evaluated in vitro for antimicrobial activity against five bacterial and two Candida species, while their cytotoxicity was tested on the normal human lung fibroblast cell line (MRC-5). Compound 1 has manifested a remarkable antifungal activity on both tested Candida strains (C. albicans and C. parapsilosis) with minimal inhibitory concentrations (MICs) of 1.43 and 11.38 µM (0.49 and 3.9 µg/mL), respectively, while no significant antimicrobial activity was observed for 2. Moreover, silver(I) coordination polymer 1 inhibits the hyphae formation of C. albicans at subinhibitory concentration. The binding affinity of both compounds 1 and 2 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy, indicating their ability to interact with these biomolecules, with compound 2 being more reactive.",
journal = "Polyhedron",
title = "Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine",
number = "1",
pages = "116585",
volume = "244",
doi = "10.1016/j.poly.2023.116585"
}

Ašanin, D., Andrejević, T., Nenadović, M., Rodić, M., Vojnović, S., Djuran, M.,& Glišić, B.. (2023). Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine. in Polyhedron, 244(1), 116585.
https://doi.org/10.1016/j.poly.2023.116585

Ašanin D, Andrejević T, Nenadović M, Rodić M, Vojnović S, Djuran M, Glišić B. Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine. in Polyhedron. 2023;244(1):116585.
doi:10.1016/j.poly.2023.116585 .

Ašanin, Darko, Andrejević, Tina, Nenadović, Marija, Rodić, Marko, Vojnović, Sandra, Djuran, Miloš, Glišić, Biljana, "Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine" in Polyhedron, 244, no. 1 (2023):116585,
https://doi.org/10.1016/j.poly.2023.116585 . .

TY  - CONF
AU  - Marija, Nenadović
AU  - Pantelić, Brana
AU  - Ponjavić, Marijana
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1916
AB  - Polyhydroxyalkanoates (PHA) are a green substitute for conventional plastics,
owing to their biological origin, biodegradability, biocompatibility and structural
diversity. However, environmental biodegradation of PHA is achieved in a time
frame of several months to several years, depending on environmental conditions,
and properties of both PHA and PHA degrading enzymes (PhaZ) [1]. Taking into
account the high production cost of PHA, landfilling at the end of life is not likely to
be cost-effective, so enzymatic biodegradation as an alternative offers an ecofriendly
bio-cyclable route to cost-effective PHA. Our study aims to tailor PhaZ
properties to create suitable biocatalysts for the industrially relevant time frame and
operating conditions. In order to do so, we decided to randomize PhaZ sequences
and functionally screen enzyme variants for accelerated PHA degradation and
improved biocatalyst stability. Up to this day, various phaZ genes have been mutated
solely for mechanistic purposes eg. Catalytic residue identification, and elucidation
of the substrate recognition process [2,3,4,5,6,7].
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
T1  - Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers
EP  - 196
SP  - 195
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1916
ER  - 

@conference{
author = "Marija, Nenadović and Pantelić, Brana and Ponjavić, Marijana and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Polyhydroxyalkanoates (PHA) are a green substitute for conventional plastics,
owing to their biological origin, biodegradability, biocompatibility and structural
diversity. However, environmental biodegradation of PHA is achieved in a time
frame of several months to several years, depending on environmental conditions,
and properties of both PHA and PHA degrading enzymes (PhaZ) [1]. Taking into
account the high production cost of PHA, landfilling at the end of life is not likely to
be cost-effective, so enzymatic biodegradation as an alternative offers an ecofriendly
bio-cyclable route to cost-effective PHA. Our study aims to tailor PhaZ
properties to create suitable biocatalysts for the industrially relevant time frame and
operating conditions. In order to do so, we decided to randomize PhaZ sequences
and functionally screen enzyme variants for accelerated PHA degradation and
improved biocatalyst stability. Up to this day, various phaZ genes have been mutated
solely for mechanistic purposes eg. Catalytic residue identification, and elucidation
of the substrate recognition process [2,3,4,5,6,7].",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023",
title = "Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers",
pages = "196-195",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1916"
}

Marija, N., Pantelić, B., Ponjavić, M.,& Nikodinović-Runić, J.. (2023). Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
Beograd : Srpsko hemijsko društvo., 9, 195-196.
https://hdl.handle.net/21.15107/rcub_imagine_1916

Marija N, Pantelić B, Ponjavić M, Nikodinović-Runić J. Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023. 2023;9:195-196.
https://hdl.handle.net/21.15107/rcub_imagine_1916 .

Marija, Nenadović, Pantelić, Brana, Ponjavić, Marijana, Nikodinović-Runić, Jasmina, "Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers" in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023, 9 (2023):195-196,
https://hdl.handle.net/21.15107/rcub_imagine_1916 .

TY  - JOUR
AU  - Ašanin, Darko
AU  - Andrejević, Tina
AU  - Nenadović, Marija
AU  - Rodić, Marko
AU  - Vojnović, Sandra
AU  - Djuran, Miloš
AU  - Glišić, Biljana
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0277538723003078
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2076
AB  - In the present study, synthesis of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine (1,6-naph), {[Ag(1,6-naph)(H2O)](BF4)}n (1) and [AuCl3(1,6-naph)] (2), was reported. The methods used for the structural characterization of a new compound 1 included IR, NMR (1H and 13C) and UV-Vis spectroscopy, cyclic voltammetry and single-crystal X-ray diffraction analysis. The crystallographic results showed that compound 1 represents silver(I) coordination polymer, in which 1,6-naph ligand acts as a bidentate bridging ligand connecting two Ag(I) ions via its N1 and N6 nitrogen atoms, while the third coordination site of the metal ion is occupied by the water oxygen atom, resulted in a T-shape geometry. Compounds 1 and 2 were evaluated in vitro for antimicrobial activity against five bacterial and two Candida species, while their cytotoxicity was tested on the normal human lung fibroblast cell line (MRC-5). Compound 1 has manifested a remarkable antifungal activity on both tested Candida strains (C. albicans and C. parapsilosis) with minimal inhibitory concentrations (MICs) of 1.43 and 11.38 µM (0.49 and 3.9 µg/mL), respectively, while no significant antimicrobial activity was observed for 2. Moreover, silver(I) coordination polymer 1 inhibits the hyphae formation of C. albicans at subinhibitory concentration. The binding affinity of both compounds 1 and 2 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy, indicating their ability to interact with these biomolecules, with compound 2 being more reactive.
T2  - Polyhedron
T1  - Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine
IS  - 1
SP  - 116585
VL  - 244
DO  - 10.1016/j.poly.2023.116585
ER  - 

@article{
author = "Ašanin, Darko and Andrejević, Tina and Nenadović, Marija and Rodić, Marko and Vojnović, Sandra and Djuran, Miloš and Glišić, Biljana",
year = "2023",
abstract = "In the present study, synthesis of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine (1,6-naph), {[Ag(1,6-naph)(H2O)](BF4)}n (1) and [AuCl3(1,6-naph)] (2), was reported. The methods used for the structural characterization of a new compound 1 included IR, NMR (1H and 13C) and UV-Vis spectroscopy, cyclic voltammetry and single-crystal X-ray diffraction analysis. The crystallographic results showed that compound 1 represents silver(I) coordination polymer, in which 1,6-naph ligand acts as a bidentate bridging ligand connecting two Ag(I) ions via its N1 and N6 nitrogen atoms, while the third coordination site of the metal ion is occupied by the water oxygen atom, resulted in a T-shape geometry. Compounds 1 and 2 were evaluated in vitro for antimicrobial activity against five bacterial and two Candida species, while their cytotoxicity was tested on the normal human lung fibroblast cell line (MRC-5). Compound 1 has manifested a remarkable antifungal activity on both tested Candida strains (C. albicans and C. parapsilosis) with minimal inhibitory concentrations (MICs) of 1.43 and 11.38 µM (0.49 and 3.9 µg/mL), respectively, while no significant antimicrobial activity was observed for 2. Moreover, silver(I) coordination polymer 1 inhibits the hyphae formation of C. albicans at subinhibitory concentration. The binding affinity of both compounds 1 and 2 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy, indicating their ability to interact with these biomolecules, with compound 2 being more reactive.",
journal = "Polyhedron",
title = "Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine",
number = "1",
pages = "116585",
volume = "244",
doi = "10.1016/j.poly.2023.116585"
}

Ašanin, D., Andrejević, T., Nenadović, M., Rodić, M., Vojnović, S., Djuran, M.,& Glišić, B.. (2023). Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine. in Polyhedron, 244(1), 116585.
https://doi.org/10.1016/j.poly.2023.116585

Ašanin D, Andrejević T, Nenadović M, Rodić M, Vojnović S, Djuran M, Glišić B. Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine. in Polyhedron. 2023;244(1):116585.
doi:10.1016/j.poly.2023.116585 .

Ašanin, Darko, Andrejević, Tina, Nenadović, Marija, Rodić, Marko, Vojnović, Sandra, Djuran, Miloš, Glišić, Biljana, "Comparative study of antimicrobial potential and DNA/BSA binding affinity of silver(I) and gold(III) coordination compounds with 1,6-naphthyridine" in Polyhedron, 244, no. 1 (2023):116585,
https://doi.org/10.1016/j.poly.2023.116585 . .

TY  - JOUR
AU  - Sourkouni, Georgia
AU  - Jeremić, Sanja
AU  - Kalogirou, Charalampia
AU  - Höfft, Oliver
AU  - Nenadović, Marija
AU  - Janković, Vukašin
AU  - Rajasekaran, Divya
AU  - Pandis, Pavlos
AU  - Padamati, Ramesh
AU  - Nikodinović-Runić, Jasmina
AU  - Argirusis, Christos
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1804
AB  - It is well acknowledged that microplastics are a major environmental problem and that the use of plastics, both petro- and bio- based, should be reduced. Nevertheless, it is also a necessity to reduce the amount of the already spread plastics. These cannot be easily degraded in the nature and accumulate in the food supply chain with major danger for animals and human life. It has been shown in the literature that advanced oxidation processes (AOPs) modify the surface of polylactic acid (PLA) materials in a way that bacteria more efficiently dock on their surface and eventually degrade them. In the present work we investigated the influence of different AOPs (ultrasounds, ultraviolet irradiation, and their combination) on the biodegradability of PLA films treated for different times between 1 and 6 h. The pre-treated samples have been degraded using a home model compost as well as a cocktail of commercial enzymes at mesophilic temperatures (37 °C and 42 °C, respectively). Degradation degree has been measured and degradation products have been identified. Excellent degradation of PLA films has been achieved with enzyme cocktail containing commercial alkaline proteases and lipases of up to 90% weight loss. For the first time, we also report valorization of PLA into bacterial nanocellulose after enzymatic hydrolysis of the samples.
T2  - World Journal of Microbiology and Biotechnology
T2  - World Journal of Microbiology and BiotechnologyWorld J Microbiol Biotechnol
T1  - Study of PLA pre-treatment, enzymatic and model-compost degradation, and valorization of degradation products to bacterial nanocellulose
IS  - 6
SP  - 161
VL  - 39
DO  - 10.1007/s11274-023-03605-4
ER  - 

@article{
author = "Sourkouni, Georgia and Jeremić, Sanja and Kalogirou, Charalampia and Höfft, Oliver and Nenadović, Marija and Janković, Vukašin and Rajasekaran, Divya and Pandis, Pavlos and Padamati, Ramesh and Nikodinović-Runić, Jasmina and Argirusis, Christos",
year = "2023",
abstract = "It is well acknowledged that microplastics are a major environmental problem and that the use of plastics, both petro- and bio- based, should be reduced. Nevertheless, it is also a necessity to reduce the amount of the already spread plastics. These cannot be easily degraded in the nature and accumulate in the food supply chain with major danger for animals and human life. It has been shown in the literature that advanced oxidation processes (AOPs) modify the surface of polylactic acid (PLA) materials in a way that bacteria more efficiently dock on their surface and eventually degrade them. In the present work we investigated the influence of different AOPs (ultrasounds, ultraviolet irradiation, and their combination) on the biodegradability of PLA films treated for different times between 1 and 6 h. The pre-treated samples have been degraded using a home model compost as well as a cocktail of commercial enzymes at mesophilic temperatures (37 °C and 42 °C, respectively). Degradation degree has been measured and degradation products have been identified. Excellent degradation of PLA films has been achieved with enzyme cocktail containing commercial alkaline proteases and lipases of up to 90% weight loss. For the first time, we also report valorization of PLA into bacterial nanocellulose after enzymatic hydrolysis of the samples.",
journal = "World Journal of Microbiology and Biotechnology, World Journal of Microbiology and BiotechnologyWorld J Microbiol Biotechnol",
title = "Study of PLA pre-treatment, enzymatic and model-compost degradation, and valorization of degradation products to bacterial nanocellulose",
number = "6",
pages = "161",
volume = "39",
doi = "10.1007/s11274-023-03605-4"
}

Sourkouni, G., Jeremić, S., Kalogirou, C., Höfft, O., Nenadović, M., Janković, V., Rajasekaran, D., Pandis, P., Padamati, R., Nikodinović-Runić, J.,& Argirusis, C.. (2023). Study of PLA pre-treatment, enzymatic and model-compost degradation, and valorization of degradation products to bacterial nanocellulose. in World Journal of Microbiology and Biotechnology, 39(6), 161.
https://doi.org/10.1007/s11274-023-03605-4

Sourkouni G, Jeremić S, Kalogirou C, Höfft O, Nenadović M, Janković V, Rajasekaran D, Pandis P, Padamati R, Nikodinović-Runić J, Argirusis C. Study of PLA pre-treatment, enzymatic and model-compost degradation, and valorization of degradation products to bacterial nanocellulose. in World Journal of Microbiology and Biotechnology. 2023;39(6):161.
doi:10.1007/s11274-023-03605-4 .

Sourkouni, Georgia, Jeremić, Sanja, Kalogirou, Charalampia, Höfft, Oliver, Nenadović, Marija, Janković, Vukašin, Rajasekaran, Divya, Pandis, Pavlos, Padamati, Ramesh, Nikodinović-Runić, Jasmina, Argirusis, Christos, "Study of PLA pre-treatment, enzymatic and model-compost degradation, and valorization of degradation products to bacterial nanocellulose" in World Journal of Microbiology and Biotechnology, 39, no. 6 (2023):161,
https://doi.org/10.1007/s11274-023-03605-4 . .

TY  - CONF
AU  - Janković, Vukašin
AU  - Nikodinović-Runić, Jasmina
AU  - Radetić, Maja
AU  - Marković, Darka
AU  - Stevanović, Milena
AU  - Nenadović, Marija
AU  - Ilić-Tomić, Tatjana
PY  - 2023
UR  - https://www.fems2023.org/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2026
AB  - Microorganisms, especially soil-dwelling Streptomyces, have the potential to both degrade
and colour a variety of textiles. Pigments from Streptomycetes could serve as colouring
agents for different natural and synthetic fabrics. Apart from pigments, Streptomyces can
produce a variety of enzymes. Several of these enzymes show favourable application in the
depolymerization of synthetic materials such as polyamide and polyurethane.
The aim of this study was the assessment of live interactions of pigmented Streptomyces
strains from the lab collection using polyamide (PA) and Polyamide/Elastane (PA/EA) knits
as substrates.
Cultivation of pigment-producing Streptomyces strains was done following the standard
microbiological protocols, using two different growth media with the addition of PA and
PA/EA knits into flasks. Cultures were incubated at 30°C for 7 and 14 days under static and
dynamic conditions. Materials were recovered and their colour coordinates, colour difference
(ΔE), and fastness were determined, and their surface changes were examined by Scanning
Electron Microscopy (SEM).
The incubation of knits with living bacterial cultures resulted in both live dyeing and
degradation, depending on the strain used. The intensity of color yield was larger under
dynamic culture conditions. Therefore, Streptomyces strains could be successfully applied in
the development of greener dyeing and degradation bioprocesses.
C3  - FEMS2023 Congress of European Microbiologists
T1  - Microbial live interactions with textiles
EP  - 835
EP  - 835
SP  - 835
VL  - 10
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2026
ER  - 

@conference{
author = "Janković, Vukašin and Nikodinović-Runić, Jasmina and Radetić, Maja and Marković, Darka and Stevanović, Milena and Nenadović, Marija and Ilić-Tomić, Tatjana",
year = "2023",
abstract = "Microorganisms, especially soil-dwelling Streptomyces, have the potential to both degrade
and colour a variety of textiles. Pigments from Streptomycetes could serve as colouring
agents for different natural and synthetic fabrics. Apart from pigments, Streptomyces can
produce a variety of enzymes. Several of these enzymes show favourable application in the
depolymerization of synthetic materials such as polyamide and polyurethane.
The aim of this study was the assessment of live interactions of pigmented Streptomyces
strains from the lab collection using polyamide (PA) and Polyamide/Elastane (PA/EA) knits
as substrates.
Cultivation of pigment-producing Streptomyces strains was done following the standard
microbiological protocols, using two different growth media with the addition of PA and
PA/EA knits into flasks. Cultures were incubated at 30°C for 7 and 14 days under static and
dynamic conditions. Materials were recovered and their colour coordinates, colour difference
(ΔE), and fastness were determined, and their surface changes were examined by Scanning
Electron Microscopy (SEM).
The incubation of knits with living bacterial cultures resulted in both live dyeing and
degradation, depending on the strain used. The intensity of color yield was larger under
dynamic culture conditions. Therefore, Streptomyces strains could be successfully applied in
the development of greener dyeing and degradation bioprocesses.",
journal = "FEMS2023 Congress of European Microbiologists",
title = "Microbial live interactions with textiles",
pages = "835-835-835",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2026"
}

Janković, V., Nikodinović-Runić, J., Radetić, M., Marković, D., Stevanović, M., Nenadović, M.,& Ilić-Tomić, T.. (2023). Microbial live interactions with textiles. in FEMS2023 Congress of European Microbiologists, 10, 835-835.
https://hdl.handle.net/21.15107/rcub_imagine_2026

Janković V, Nikodinović-Runić J, Radetić M, Marković D, Stevanović M, Nenadović M, Ilić-Tomić T. Microbial live interactions with textiles. in FEMS2023 Congress of European Microbiologists. 2023;10:835-835.
https://hdl.handle.net/21.15107/rcub_imagine_2026 .

Janković, Vukašin, Nikodinović-Runić, Jasmina, Radetić, Maja, Marković, Darka, Stevanović, Milena, Nenadović, Marija, Ilić-Tomić, Tatjana, "Microbial live interactions with textiles" in FEMS2023 Congress of European Microbiologists, 10 (2023):835-835,
https://hdl.handle.net/21.15107/rcub_imagine_2026 .