TY  - JOUR
AU  - Prlainović, Nevena
AU  - Milovanović, Jelena
AU  - Milašinović, Nikola
AU  - Bezbradica, Dejan
AU  - Mijin, Dušan
PY  - 2024
UR  - https://doiserbia.nb.rs/Article.aspx?ID=0367-598X2400003P
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2311
AB  - Lipase-catalyzed organic reactions have been widely practiced in the past three decades. Especially interesting are insoluble/immobilized forms due to providing a possibility of facile use and recyclability, thus reducing process costs, and making the procedure more environmentally friendly. Carbon-based supports have been extensively exploited for this purpose, because of neutral and biodegradable nature and thermal and chemical stability. Their high specific surface area, characteristic surface morphology and lower mass transfer resistances play a vital role in the performance of the attached enzyme. This review paper presents an overview of the main aspects of lipase immobilized on multi-walled carbon nanotubes (MWCNTs). Moreover, different immobilization strategies to achieve a biocatalyst with improved performances are discussed. Furthermore, as lipases are considered to have high commercial worth for synthesis of valuable organic molecules, the second part of the paper is dedicated to the overview of the most important industrial sectors in which these nanobiocatalysts have been used. In specific, applications in biodiesel production, flavour ester synthesis and racemization are summarized.
AB  - Lipaze su poslednjih decenija široko rasprostanjeni katalizatoriu raznovrsnim organskim reakcijama. Posebno su interesantne u imobilisanom/nerastvornom obliku jer je na ovaj način olakšana njihova upotreba uz mogućnost recikliranja i ponovne upotrebe čime se smanjuju troškovi samog procesa i postupak je ekološki prihvatljiviji. Kao nosači za vezivanje nanomaterijali na bazi ugljenika, posebno ugljenične nanocevi, su našli primenu zbog svojih izuzetnih fizičkih, mehaničkih i hemijskih svojstava. Njihova velika specifična površina, karakteristična površinska morfologija i smanjen otpor prenosu mase igraju vitalnu ulogu u performansama vezanog enzima. Ovaj pregledni rad predstavlja prikaz glavnih aspekata lipaze imobilisane na višeslojne ugljenične nanocevi i različitih strategija imobilizacije za dobijanje biokatalizatora sa poboljšanim svojstvima. Takođe, kako su lipaze enzimi od velikog komercijalnog značaja za organsku sintezu i primenu u biotehnologiji, drugi deo rada posvećen je pregledu najvažnijih industrijskih sektora u kojima su ovi nanobiokatalizatori našli primenu. Shodno tome, dat je pregled proizvodnje biodizela, mirisnih estara i racemizacije
PB  - Association of the Chemical Engineers of Serbia
T2  - Hemijska industrija
T2  - Hemijska industrija
T1  - Multi-walled carbon nanotubes as lipase carriers for organic synthesis: current trends and recent update
T1  - Višeslojne ugljenične nanocevi kao nosač lipaze za organsku sintezu: pregled najnovijih trendova
IS  - n/a
DO  - 10.2298/HEMIND230614003P
ER  - 

@article{
author = "Prlainović, Nevena and Milovanović, Jelena and Milašinović, Nikola and Bezbradica, Dejan and Mijin, Dušan",
year = "2024",
abstract = "Lipase-catalyzed organic reactions have been widely practiced in the past three decades. Especially interesting are insoluble/immobilized forms due to providing a possibility of facile use and recyclability, thus reducing process costs, and making the procedure more environmentally friendly. Carbon-based supports have been extensively exploited for this purpose, because of neutral and biodegradable nature and thermal and chemical stability. Their high specific surface area, characteristic surface morphology and lower mass transfer resistances play a vital role in the performance of the attached enzyme. This review paper presents an overview of the main aspects of lipase immobilized on multi-walled carbon nanotubes (MWCNTs). Moreover, different immobilization strategies to achieve a biocatalyst with improved performances are discussed. Furthermore, as lipases are considered to have high commercial worth for synthesis of valuable organic molecules, the second part of the paper is dedicated to the overview of the most important industrial sectors in which these nanobiocatalysts have been used. In specific, applications in biodiesel production, flavour ester synthesis and racemization are summarized., Lipaze su poslednjih decenija široko rasprostanjeni katalizatoriu raznovrsnim organskim reakcijama. Posebno su interesantne u imobilisanom/nerastvornom obliku jer je na ovaj način olakšana njihova upotreba uz mogućnost recikliranja i ponovne upotrebe čime se smanjuju troškovi samog procesa i postupak je ekološki prihvatljiviji. Kao nosači za vezivanje nanomaterijali na bazi ugljenika, posebno ugljenične nanocevi, su našli primenu zbog svojih izuzetnih fizičkih, mehaničkih i hemijskih svojstava. Njihova velika specifična površina, karakteristična površinska morfologija i smanjen otpor prenosu mase igraju vitalnu ulogu u performansama vezanog enzima. Ovaj pregledni rad predstavlja prikaz glavnih aspekata lipaze imobilisane na višeslojne ugljenične nanocevi i različitih strategija imobilizacije za dobijanje biokatalizatora sa poboljšanim svojstvima. Takođe, kako su lipaze enzimi od velikog komercijalnog značaja za organsku sintezu i primenu u biotehnologiji, drugi deo rada posvećen je pregledu najvažnijih industrijskih sektora u kojima su ovi nanobiokatalizatori našli primenu. Shodno tome, dat je pregled proizvodnje biodizela, mirisnih estara i racemizacije",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Hemijska industrija, Hemijska industrija",
title = "Multi-walled carbon nanotubes as lipase carriers for organic synthesis: current trends and recent update, Višeslojne ugljenične nanocevi kao nosač lipaze za organsku sintezu: pregled najnovijih trendova",
number = "n/a",
doi = "10.2298/HEMIND230614003P"
}

Prlainović, N., Milovanović, J., Milašinović, N., Bezbradica, D.,& Mijin, D.. (2024). Multi-walled carbon nanotubes as lipase carriers for organic synthesis: current trends and recent update. in Hemijska industrija
Association of the Chemical Engineers of Serbia.(n/a).
https://doi.org/10.2298/HEMIND230614003P

Prlainović N, Milovanović J, Milašinović N, Bezbradica D, Mijin D. Multi-walled carbon nanotubes as lipase carriers for organic synthesis: current trends and recent update. in Hemijska industrija. 2024;(n/a).
doi:10.2298/HEMIND230614003P .

Prlainović, Nevena, Milovanović, Jelena, Milašinović, Nikola, Bezbradica, Dejan, Mijin, Dušan, "Multi-walled carbon nanotubes as lipase carriers for organic synthesis: current trends and recent update" in Hemijska industrija, no. n/a (2024),
https://doi.org/10.2298/HEMIND230614003P . .

TY  - CONF
AU  - Ponjavić, Marijana
AU  - Milovanović, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Đurašković, Nikola
AU  - Tica, Nedeljko
AU  - Živković, Irena
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2334
AB  - In this paper, the biodegradability of three SferiCorn™
biocomposites was investigated. Corn stover prepared through eco-friendly
washing and grinding to short fibers was used as reinforcement. Three different
biopolymers, corn starch, alginate and poly(3-hydroxybutyrate-co-3-
hydroxyvalerate) (PHBV), were used as matrices. The biodegradability of
prepared composites was investigated in a simulated soil burial test and the
samples were characterized in terms of weight loss and morphological changes. It
was shown that biodegradability can be tuned by biopolymers used as matrices.
Changes in the surface morphology after biodegradation of tested samples were
confirmed using scanning electron microscopy (SEM).
PB  - Čačak : Faculty of Agronomy
C3  - 2nd International symposium on biotechnology
T1  - Biodegradability assessment of corn stover reinforced composite materials with different matrix
EP  - 261
SP  - 225
DO  - 10.46793/SBT29
ER  - 

@conference{
author = "Ponjavić, Marijana and Milovanović, Jelena and Nikodinović-Runić, Jasmina and Đurašković, Nikola and Tica, Nedeljko and Živković, Irena",
year = "2024",
abstract = "In this paper, the biodegradability of three SferiCorn™
biocomposites was investigated. Corn stover prepared through eco-friendly
washing and grinding to short fibers was used as reinforcement. Three different
biopolymers, corn starch, alginate and poly(3-hydroxybutyrate-co-3-
hydroxyvalerate) (PHBV), were used as matrices. The biodegradability of
prepared composites was investigated in a simulated soil burial test and the
samples were characterized in terms of weight loss and morphological changes. It
was shown that biodegradability can be tuned by biopolymers used as matrices.
Changes in the surface morphology after biodegradation of tested samples were
confirmed using scanning electron microscopy (SEM).",
publisher = "Čačak : Faculty of Agronomy",
journal = "2nd International symposium on biotechnology",
title = "Biodegradability assessment of corn stover reinforced composite materials with different matrix",
pages = "261-225",
doi = "10.46793/SBT29"
}

Ponjavić, M., Milovanović, J., Nikodinović-Runić, J., Đurašković, N., Tica, N.,& Živković, I.. (2024). Biodegradability assessment of corn stover reinforced composite materials with different matrix. in 2nd International symposium on biotechnology
Čačak : Faculty of Agronomy., 225-261.
https://doi.org/10.46793/SBT29

Ponjavić M, Milovanović J, Nikodinović-Runić J, Đurašković N, Tica N, Živković I. Biodegradability assessment of corn stover reinforced composite materials with different matrix. in 2nd International symposium on biotechnology. 2024;:225-261.
doi:10.46793/SBT29 .

Ponjavić, Marijana, Milovanović, Jelena, Nikodinović-Runić, Jasmina, Đurašković, Nikola, Tica, Nedeljko, Živković, Irena, "Biodegradability assessment of corn stover reinforced composite materials with different matrix" in 2nd International symposium on biotechnology (2024):225-261,
https://doi.org/10.46793/SBT29 . .

TY  - JOUR
AU  - Lazić, Jelena
AU  - Filipović, Vuk
AU  - Pantelić, Lena
AU  - Milovanović, Jelena
AU  - Vojnović, Sandra
AU  - Nikodinović-Runić, Jasmina
PY  - 2024
UR  - https://www.frontiersin.org/articles/10.3389/fbioe.2024.1351583
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2387
AB  - Bacterial natural products (BNPs) have been and still are very important sources of leads for drug development and chemical novelty. The possibility to perform late-stage diversification of these biomolecules is an attractive approach to obtain novel structures by means other than total chemical synthesis or metal complexation reactions. Although biocatalysis is gaining popularity within the context of green chemistry, a vast majority of the sequenced genome data aimed at deciphering metabolic pathways for BNP biosynthesis, possibly containing additional tailoring enzymes, remains underexplored. In this review, we report a systematic overview of biotransformations of 21 molecules, which include derivatization by halogenation, esterification, reduction, oxidation, alkylation and nitration reactions, as well as degradation products as their sub-derivatives. These BNPs were grouped based on their biological activities into antibacterial (5), antifungal (5), anticancer (5), immunosuppressive (2) and quorum sensing modulating (4) compounds. This study summarized 73 derivatives and 16 degradation sub-derivatives originating from 12 BNPs. The remaining 9 BNPs have not been reported as substrates in biotransformation reactions. The highest number of 28 biocatalytic reactions was reported for the antibacterial drug vancomycin, followed by 18 reactions reported for the immunosuppressive drug rapamycin, suggesting that research focus to date was more on BNPs that were already in clinical use. The most common biocatalysts include oxidoreductases, transferases, lipases, isomerases and haloperoxidases. This review highlights biocatalytic routes for the late-stage diversification reactions of BNPs, which potentially help to recognize the structural optimizations of bioactive scaffolds for the generation of new biomolecules, eventually leading to drug development.
PB  - Frontiers
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Late-stage diversification of bacterial natural products through biocatalysis
VL  - 12
DO  - 10.3389/fbioe.2024.1351583
ER  - 

@article{
author = "Lazić, Jelena and Filipović, Vuk and Pantelić, Lena and Milovanović, Jelena and Vojnović, Sandra and Nikodinović-Runić, Jasmina",
year = "2024",
abstract = "Bacterial natural products (BNPs) have been and still are very important sources of leads for drug development and chemical novelty. The possibility to perform late-stage diversification of these biomolecules is an attractive approach to obtain novel structures by means other than total chemical synthesis or metal complexation reactions. Although biocatalysis is gaining popularity within the context of green chemistry, a vast majority of the sequenced genome data aimed at deciphering metabolic pathways for BNP biosynthesis, possibly containing additional tailoring enzymes, remains underexplored. In this review, we report a systematic overview of biotransformations of 21 molecules, which include derivatization by halogenation, esterification, reduction, oxidation, alkylation and nitration reactions, as well as degradation products as their sub-derivatives. These BNPs were grouped based on their biological activities into antibacterial (5), antifungal (5), anticancer (5), immunosuppressive (2) and quorum sensing modulating (4) compounds. This study summarized 73 derivatives and 16 degradation sub-derivatives originating from 12 BNPs. The remaining 9 BNPs have not been reported as substrates in biotransformation reactions. The highest number of 28 biocatalytic reactions was reported for the antibacterial drug vancomycin, followed by 18 reactions reported for the immunosuppressive drug rapamycin, suggesting that research focus to date was more on BNPs that were already in clinical use. The most common biocatalysts include oxidoreductases, transferases, lipases, isomerases and haloperoxidases. This review highlights biocatalytic routes for the late-stage diversification reactions of BNPs, which potentially help to recognize the structural optimizations of bioactive scaffolds for the generation of new biomolecules, eventually leading to drug development.",
publisher = "Frontiers",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Late-stage diversification of bacterial natural products through biocatalysis",
volume = "12",
doi = "10.3389/fbioe.2024.1351583"
}

Lazić, J., Filipović, V., Pantelić, L., Milovanović, J., Vojnović, S.,& Nikodinović-Runić, J.. (2024). Late-stage diversification of bacterial natural products through biocatalysis. in Frontiers in Bioengineering and Biotechnology
Frontiers., 12.
https://doi.org/10.3389/fbioe.2024.1351583

Lazić J, Filipović V, Pantelić L, Milovanović J, Vojnović S, Nikodinović-Runić J. Late-stage diversification of bacterial natural products through biocatalysis. in Frontiers in Bioengineering and Biotechnology. 2024;12.
doi:10.3389/fbioe.2024.1351583 .

Lazić, Jelena, Filipović, Vuk, Pantelić, Lena, Milovanović, Jelena, Vojnović, Sandra, Nikodinović-Runić, Jasmina, "Late-stage diversification of bacterial natural products through biocatalysis" in Frontiers in Bioengineering and Biotechnology, 12 (2024),
https://doi.org/10.3389/fbioe.2024.1351583 . .

TY  - JOUR
AU  - Škaro Bogojević, Sanja
AU  - Perminova, D
AU  - Jaksic, J
AU  - Milcic, M
AU  - Medakovic, V
AU  - Milovanović, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Maslak, V
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0022286023023797
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2274
AB  - Poly(ethylene terephthalate) (PET) is widely used material in the healthcare due to its mechanical properties including resistance to chemicals and abrasion. However, it is susceptible to bacterial attachment and contamination. This study addresses some newly designed model compounds of PET with antimicrobial properties that could potentially be incorporated into PET materials. All compounds were synthesized for the first time by labeling an integral part of PET with chromophores in the form of esters of cinnamic and ferulic acids. After complete structural characterization, the effect of new compounds on microbial growth and communication (quorum sensing, QS) was analyzed and further investigated using molecular docking. The obtained results indicate that the introduction of chromophores that have one part of cinnamic acid enriched with a methoxy functional group in them acts as QS modulators. Moreover, compounds exhibited dose-dependent selectivity toward QS signaling pathways and the highest tested concentration of compounds showed Pseudomonas Quinolone Signal (PQS) inhibitory activity suggesting that these compounds have a potential effect on pyocyanin production. Docking studies demonstrated that compounds hold binding power to all four QS protein targets (LuxP, periplasmatic protein that binds AI-2 inducer and forms a complex able to transduce the autoinducer signal, RhIR protein that is a key QS transcriptional regulator that activates the genes involved in the synthesis of rhamnolipids and pyocyanin, AbaI protein that has a role in QS signal transduction, and LasR protein which is a key QS transcriptional regulator that activates transcription of genes coding for some virulence-associated traits) while the highest binding strength is observed with compounds 2 and 6 containing single cinnamic acid fragment, suggesting their further biomedical application.
T2  - Journal of Molecular Structure
T1  - Novel cinnamic acid-based PET derivatives as quorum sensing modulators
SP  - 137291
VL  - 1300
DO  - 10.1016/j.molstruc.2023.137291
ER  - 

@article{
author = "Škaro Bogojević, Sanja and Perminova, D and Jaksic, J and Milcic, M and Medakovic, V and Milovanović, Jelena and Nikodinović-Runić, Jasmina and Maslak, V",
year = "2024",
abstract = "Poly(ethylene terephthalate) (PET) is widely used material in the healthcare due to its mechanical properties including resistance to chemicals and abrasion. However, it is susceptible to bacterial attachment and contamination. This study addresses some newly designed model compounds of PET with antimicrobial properties that could potentially be incorporated into PET materials. All compounds were synthesized for the first time by labeling an integral part of PET with chromophores in the form of esters of cinnamic and ferulic acids. After complete structural characterization, the effect of new compounds on microbial growth and communication (quorum sensing, QS) was analyzed and further investigated using molecular docking. The obtained results indicate that the introduction of chromophores that have one part of cinnamic acid enriched with a methoxy functional group in them acts as QS modulators. Moreover, compounds exhibited dose-dependent selectivity toward QS signaling pathways and the highest tested concentration of compounds showed Pseudomonas Quinolone Signal (PQS) inhibitory activity suggesting that these compounds have a potential effect on pyocyanin production. Docking studies demonstrated that compounds hold binding power to all four QS protein targets (LuxP, periplasmatic protein that binds AI-2 inducer and forms a complex able to transduce the autoinducer signal, RhIR protein that is a key QS transcriptional regulator that activates the genes involved in the synthesis of rhamnolipids and pyocyanin, AbaI protein that has a role in QS signal transduction, and LasR protein which is a key QS transcriptional regulator that activates transcription of genes coding for some virulence-associated traits) while the highest binding strength is observed with compounds 2 and 6 containing single cinnamic acid fragment, suggesting their further biomedical application.",
journal = "Journal of Molecular Structure",
title = "Novel cinnamic acid-based PET derivatives as quorum sensing modulators",
pages = "137291",
volume = "1300",
doi = "10.1016/j.molstruc.2023.137291"
}

Škaro Bogojević, S., Perminova, D., Jaksic, J., Milcic, M., Medakovic, V., Milovanović, J., Nikodinović-Runić, J.,& Maslak, V.. (2024). Novel cinnamic acid-based PET derivatives as quorum sensing modulators. in Journal of Molecular Structure, 1300, 137291.
https://doi.org/10.1016/j.molstruc.2023.137291

Škaro Bogojević S, Perminova D, Jaksic J, Milcic M, Medakovic V, Milovanović J, Nikodinović-Runić J, Maslak V. Novel cinnamic acid-based PET derivatives as quorum sensing modulators. in Journal of Molecular Structure. 2024;1300:137291.
doi:10.1016/j.molstruc.2023.137291 .

Škaro Bogojević, Sanja, Perminova, D, Jaksic, J, Milcic, M, Medakovic, V, Milovanović, Jelena, Nikodinović-Runić, Jasmina, Maslak, V, "Novel cinnamic acid-based PET derivatives as quorum sensing modulators" in Journal of Molecular Structure, 1300 (2024):137291,
https://doi.org/10.1016/j.molstruc.2023.137291 . .

TY  - JOUR
AU  - Mitrović, Aleksandra
AU  - Milovanović, Jelena
AU  - Gurgul, Jacek
AU  - Žekić, Andrijana
AU  - Nikodinović-Runić, Jasmina
AU  - Maslak, Veselin
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0141022923001011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1984
AB  - We present a novel approach for the enzymatic functionalization of graphene, utilizing horseradish peroxidase (HPO) and laccase (LC) from Trametes versicolor. This study demonstrates, for the first time, the covalent modification of non-homogeneous graphene with a low surface-to-volume ratio, both in solution and on solid support. Through thermogravimetry analysis, we estimate the degree of functionalization to be 11% with HPO and 4% with LC, attributed to the varying redox potentials of the enzymes. This work highlights the potential of enzymatic reactions for tailored functionalization of graphene under mild conditions.
PB  - Elsevier
T2  - Enzyme and Microbial Technology
T1  - Enzymatic functionalization of liquid phase exfoliated graphene using horseradish peroxidase and laccase
SP  - 110293
VL  - 170
DO  - 10.1016/j.enzmictec.2023.110293
ER  - 

@article{
author = "Mitrović, Aleksandra and Milovanović, Jelena and Gurgul, Jacek and Žekić, Andrijana and Nikodinović-Runić, Jasmina and Maslak, Veselin",
year = "2023",
abstract = "We present a novel approach for the enzymatic functionalization of graphene, utilizing horseradish peroxidase (HPO) and laccase (LC) from Trametes versicolor. This study demonstrates, for the first time, the covalent modification of non-homogeneous graphene with a low surface-to-volume ratio, both in solution and on solid support. Through thermogravimetry analysis, we estimate the degree of functionalization to be 11% with HPO and 4% with LC, attributed to the varying redox potentials of the enzymes. This work highlights the potential of enzymatic reactions for tailored functionalization of graphene under mild conditions.",
publisher = "Elsevier",
journal = "Enzyme and Microbial Technology",
title = "Enzymatic functionalization of liquid phase exfoliated graphene using horseradish peroxidase and laccase",
pages = "110293",
volume = "170",
doi = "10.1016/j.enzmictec.2023.110293"
}

Mitrović, A., Milovanović, J., Gurgul, J., Žekić, A., Nikodinović-Runić, J.,& Maslak, V.. (2023). Enzymatic functionalization of liquid phase exfoliated graphene using horseradish peroxidase and laccase. in Enzyme and Microbial Technology
Elsevier., 170, 110293.
https://doi.org/10.1016/j.enzmictec.2023.110293

Mitrović A, Milovanović J, Gurgul J, Žekić A, Nikodinović-Runić J, Maslak V. Enzymatic functionalization of liquid phase exfoliated graphene using horseradish peroxidase and laccase. in Enzyme and Microbial Technology. 2023;170:110293.
doi:10.1016/j.enzmictec.2023.110293 .

Mitrović, Aleksandra, Milovanović, Jelena, Gurgul, Jacek, Žekić, Andrijana, Nikodinović-Runić, Jasmina, Maslak, Veselin, "Enzymatic functionalization of liquid phase exfoliated graphene using horseradish peroxidase and laccase" in Enzyme and Microbial Technology, 170 (2023):110293,
https://doi.org/10.1016/j.enzmictec.2023.110293 . .

TY  - CONF
AU  - Rajesekhran, Divya
AU  - Ponnupandian, Siva
AU  - Bhagabati, Purabi
AU  - Milovanović, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Babu, Ramesh
PY  - 2023
UR  - https://pps2023india.com/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2286
AB  - In a world where unsustainable anthropogenic activities are drastically affecting Earth’s
ecosystems and natural resources, threatening animal (including human) health and safety, the
sustainable management and use of resources, including preventing plastic pollution, have
become of paramount importance. Plastics, being at the core of many low cost and high
performing materials, are widely used despite being based on non-renewable petrochemicals.
Currently, in EU, only 7% of plastics are recycled out of 57.2 million tonnes produced, and
93% end up in incineration, landfills and oceans, releasing persistent, bio-accumulative, toxic
and hazardous chemicals.
Bio Innovation of a Circular Economy for Plastics 1 (BioICEP) an EU Horizon project focuses
on reducing the burden of plastic waste in the environment and also changing the linear value
chains of current plastic products. The project's overall objective is to demonstrate a seamless,
sustainable route to a circular economy for plastics by developing cost-effective and low-
energy demand processes for waste plastic biotransformation into high-value marketable
products such as biodegradable plastics. To address these challenges, the BioICEP project
focuses on developing physical, enzymatic and biocatalytic pre-treatments to convert the
mixed plastic waste to biodegradable plastics focusing on recycling and upcycling of the
products with a zero-waste and regenerative approach.
C3  - Polymer Processing Society Asia-Australasia Regional Conference
T1  - Circular Plastics: Upscaling the mixed plastic waste to biodegradable  plastics
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2286
ER  - 

@conference{
author = "Rajesekhran, Divya and Ponnupandian, Siva and Bhagabati, Purabi and Milovanović, Jelena and Nikodinović-Runić, Jasmina and Babu, Ramesh",
year = "2023",
abstract = "In a world where unsustainable anthropogenic activities are drastically affecting Earth’s
ecosystems and natural resources, threatening animal (including human) health and safety, the
sustainable management and use of resources, including preventing plastic pollution, have
become of paramount importance. Plastics, being at the core of many low cost and high
performing materials, are widely used despite being based on non-renewable petrochemicals.
Currently, in EU, only 7% of plastics are recycled out of 57.2 million tonnes produced, and
93% end up in incineration, landfills and oceans, releasing persistent, bio-accumulative, toxic
and hazardous chemicals.
Bio Innovation of a Circular Economy for Plastics 1 (BioICEP) an EU Horizon project focuses
on reducing the burden of plastic waste in the environment and also changing the linear value
chains of current plastic products. The project's overall objective is to demonstrate a seamless,
sustainable route to a circular economy for plastics by developing cost-effective and low-
energy demand processes for waste plastic biotransformation into high-value marketable
products such as biodegradable plastics. To address these challenges, the BioICEP project
focuses on developing physical, enzymatic and biocatalytic pre-treatments to convert the
mixed plastic waste to biodegradable plastics focusing on recycling and upcycling of the
products with a zero-waste and regenerative approach.",
journal = "Polymer Processing Society Asia-Australasia Regional Conference",
title = "Circular Plastics: Upscaling the mixed plastic waste to biodegradable  plastics",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2286"
}

Rajesekhran, D., Ponnupandian, S., Bhagabati, P., Milovanović, J., Nikodinović-Runić, J.,& Babu, R.. (2023). Circular Plastics: Upscaling the mixed plastic waste to biodegradable  plastics. in Polymer Processing Society Asia-Australasia Regional Conference.
https://hdl.handle.net/21.15107/rcub_imagine_2286

Rajesekhran D, Ponnupandian S, Bhagabati P, Milovanović J, Nikodinović-Runić J, Babu R. Circular Plastics: Upscaling the mixed plastic waste to biodegradable  plastics. in Polymer Processing Society Asia-Australasia Regional Conference. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2286 .

Rajesekhran, Divya, Ponnupandian, Siva, Bhagabati, Purabi, Milovanović, Jelena, Nikodinović-Runić, Jasmina, Babu, Ramesh, "Circular Plastics: Upscaling the mixed plastic waste to biodegradable  plastics" in Polymer Processing Society Asia-Australasia Regional Conference (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2286 .

TY  - CONF
AU  - Milovanović, Jelena
AU  - Nenadović, Marija
AU  - Pantelić, Brana
AU  - Ponjavić,  Marijana
AU  - Sourkouni, Georgia
AU  - Kalogirou, Charalampia
AU  - Argirusis, Christos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://esabweb.org/E_CONGRESS/Poster+Programme/Day/Online/All+day/Enhanced+enzymatic+depolymerization+of+polylactic+acid+%28PLA%29+through+plasma+pretreatment+and+subsequent+conversion+to+biopolymer.html
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2232
AB  - Polylactic acid (PLA) serves as a bio-based alternative to fossil-based single-use plastics, biodegrading at high temperatures (58°C) and humidity during industrial composting. Despite enzymes' ability to catalyze reactions at near-ambient temperatures, polymer rigidity can impede efficient depolymerization. To address these challenges, we conducted a study of enzymatic PLA degradation at 42°C combined with green plasma pretreatment to help disrupt the crystalline regions within the polymer. Here we report the effect of length of plasma pretreatment on the rate of PLA degradation by enzyme mix containing commercial enzymes with reported PLA degrading activity. Results indicate that a 5-minute plasma pretreatment significantly enhances enzymatic degradation, with a 16% weight loss achieved in 4 weeks—a two-fold increase compared to untreated PLA. Furthermore, we report the valorization of PLA into bacterial nanocellulose after enzymatic hydrolysis of the samples.
PB  - European Society of Applied Biocatalysis
C3  - ESAB E-Congress
T1  - Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2232
ER  - 

@conference{
author = "Milovanović, Jelena and Nenadović, Marija and Pantelić, Brana and Ponjavić,  Marijana and Sourkouni, Georgia and Kalogirou, Charalampia and Argirusis, Christos and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Polylactic acid (PLA) serves as a bio-based alternative to fossil-based single-use plastics, biodegrading at high temperatures (58°C) and humidity during industrial composting. Despite enzymes' ability to catalyze reactions at near-ambient temperatures, polymer rigidity can impede efficient depolymerization. To address these challenges, we conducted a study of enzymatic PLA degradation at 42°C combined with green plasma pretreatment to help disrupt the crystalline regions within the polymer. Here we report the effect of length of plasma pretreatment on the rate of PLA degradation by enzyme mix containing commercial enzymes with reported PLA degrading activity. Results indicate that a 5-minute plasma pretreatment significantly enhances enzymatic degradation, with a 16% weight loss achieved in 4 weeks—a two-fold increase compared to untreated PLA. Furthermore, we report the valorization of PLA into bacterial nanocellulose after enzymatic hydrolysis of the samples.",
publisher = "European Society of Applied Biocatalysis",
journal = "ESAB E-Congress",
title = "Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2232"
}

Milovanović, J., Nenadović, M., Pantelić, B., Ponjavić, M., Sourkouni, G., Kalogirou, C., Argirusis, C.,& Nikodinović-Runić, J.. (2023). Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer. in ESAB E-Congress
European Society of Applied Biocatalysis..
https://hdl.handle.net/21.15107/rcub_imagine_2232

Milovanović J, Nenadović M, Pantelić B, Ponjavić M, Sourkouni G, Kalogirou C, Argirusis C, Nikodinović-Runić J. Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer. in ESAB E-Congress. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2232 .

Milovanović, Jelena, Nenadović, Marija, Pantelić, Brana, Ponjavić,  Marijana, Sourkouni, Georgia, Kalogirou, Charalampia, Argirusis, Christos, Nikodinović-Runić, Jasmina, "Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer" in ESAB E-Congress (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2232 .

TY  - CONF
AU  - Nenadović, Marija
AU  - Pantelić, Brana
AU  - Lazić, Jelena
AU  - Maslak, Veselin
AU  - Nikodinović-Runić, Jasmina
AU  - Milovanović, Jelena
PY  - 2023
UR  - https://afea.eventsair.com/10th-conference-of-mikrobiokosmos/abstract-book
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2237
AB  - PHAs are naturally made microbial polyesters that
have been commercialized as biodegradable
plastics. However, it has been shown that these
materials are not so easily biodegraded in natural
environments [1]. PHA depolymerases are key PHA
degrading enzymes and their identification and
characterization is of great interest and importance.
Currently, screening is done on polymeric
substrates using techniques such as clear zone
assays on agar or weight loss measurements.
Results obtained using these different methods
cannot be directly compared, since they depend
highly on the polymer used, PHA granules
preparation and assay conditions [2].
In order to design a more specific test for the
determination of PHA depolymerase activity, we
synthesized 3-hyoxyalkanoate monomers (3-HA
monomer) and 3-hyoxyalkanoic acid dimers (3-HA
dimer) and their respective p-nitrophenyl esters,
allowing for spectrophotometric determination of
their activity [3]. Compounds were characterized
using N and FTIR. Para-nitrophenyl labeled
substrates were then used in the enzymatic activity
assay with the benchmark polyhyoxyoctanoate
(PHO) depolymerase from Pseudomonas
fluorescens GK13 expressed in Escherichia coli
CodonPlus-RIPL hosts. This activity was compared
to recombinantly expressed leaf-branch compost
cutinase (LCC cutinase) and
polyethyleneterephtalate (PET) hyolyzing esterase
from Ideonella sakaiensis (IsPETase). Our initial
results revealed increased specificity of PHO
depolymerase towards newly synthetized
substrates, suggesting their suitability for specific
screens and isolation of new mcl-PHA
depolymerases, as well as in high throughput
screening assays designed for guiding their
directed evolution.
C3  - 10th Conference of Mikrobiokosmos
T1  - Medium chain length polyhyoxyalkanoates (mcl-PHA) model compounds for the discovery of novel PHA depolymerases
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2237
ER  - 

@conference{
author = "Nenadović, Marija and Pantelić, Brana and Lazić, Jelena and Maslak, Veselin and Nikodinović-Runić, Jasmina and Milovanović, Jelena",
year = "2023",
abstract = "PHAs are naturally made microbial polyesters that
have been commercialized as biodegradable
plastics. However, it has been shown that these
materials are not so easily biodegraded in natural
environments [1]. PHA depolymerases are key PHA
degrading enzymes and their identification and
characterization is of great interest and importance.
Currently, screening is done on polymeric
substrates using techniques such as clear zone
assays on agar or weight loss measurements.
Results obtained using these different methods
cannot be directly compared, since they depend
highly on the polymer used, PHA granules
preparation and assay conditions [2].
In order to design a more specific test for the
determination of PHA depolymerase activity, we
synthesized 3-hyoxyalkanoate monomers (3-HA
monomer) and 3-hyoxyalkanoic acid dimers (3-HA
dimer) and their respective p-nitrophenyl esters,
allowing for spectrophotometric determination of
their activity [3]. Compounds were characterized
using N and FTIR. Para-nitrophenyl labeled
substrates were then used in the enzymatic activity
assay with the benchmark polyhyoxyoctanoate
(PHO) depolymerase from Pseudomonas
fluorescens GK13 expressed in Escherichia coli
CodonPlus-RIPL hosts. This activity was compared
to recombinantly expressed leaf-branch compost
cutinase (LCC cutinase) and
polyethyleneterephtalate (PET) hyolyzing esterase
from Ideonella sakaiensis (IsPETase). Our initial
results revealed increased specificity of PHO
depolymerase towards newly synthetized
substrates, suggesting their suitability for specific
screens and isolation of new mcl-PHA
depolymerases, as well as in high throughput
screening assays designed for guiding their
directed evolution.",
journal = "10th Conference of Mikrobiokosmos",
title = "Medium chain length polyhyoxyalkanoates (mcl-PHA) model compounds for the discovery of novel PHA depolymerases",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2237"
}

Nenadović, M., Pantelić, B., Lazić, J., Maslak, V., Nikodinović-Runić, J.,& Milovanović, J.. (2023). Medium chain length polyhyoxyalkanoates (mcl-PHA) model compounds for the discovery of novel PHA depolymerases. in 10th Conference of Mikrobiokosmos.
https://hdl.handle.net/21.15107/rcub_imagine_2237

Nenadović M, Pantelić B, Lazić J, Maslak V, Nikodinović-Runić J, Milovanović J. Medium chain length polyhyoxyalkanoates (mcl-PHA) model compounds for the discovery of novel PHA depolymerases. in 10th Conference of Mikrobiokosmos. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2237 .

Nenadović, Marija, Pantelić, Brana, Lazić, Jelena, Maslak, Veselin, Nikodinović-Runić, Jasmina, Milovanović, Jelena, "Medium chain length polyhyoxyalkanoates (mcl-PHA) model compounds for the discovery of novel PHA depolymerases" in 10th Conference of Mikrobiokosmos (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2237 .

TY  - CONF
AU  - Ćirić, Milica
AU  - Šaraba, Vladimir
AU  - Budin, Clémence
AU  - Boer de, Tjalf
AU  - Milovanović, Jelena
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2105
AB  - Three mineral water occurrences, captured by wells with a depth of 6.5-442.5 m and used for drinking
and bathing purposes, were sampled and cultivated under conditions favouring the growth of anaerobic,
microaerophilic or CO2 bacteria, in order to capture predominantly anaerobic portion of the bacteriome, which is
dominant in water and soils. Cultivated bacteria were identified by next-generation 16S sequencing and their
biotechnological potential in plastics and lignocellulose degradation was explored. Most abundant genera
detected in examined samples mainly belong to facultative anaerobes that are common representatives of water
and soil environments. In total, 17 genera were detected with a relative abundance over 1% in all three samples,
including Aeromonas, Exiguobacterium, Comamonas and Acinetobacter. Half of the screened isolates demonstrated
growth on at least one plastic or lignocellulosic polymer, with one isolate demonstrating growth on all tested
substrates, one demonstrating carboxymethyl cellulose- and one arabinoxylan-degrading ability. Some of the
representatives of genera identified with high relative abundance in mineral water samples, such as Aeromonas,
Klebsiella, Escherichia, Salmonella, Enterobacter, Pseudomonas and Staphylococcus, have been previously documented
to have pathogenic potential. Due to the use of investigated mineral waters for drinking and bathing, the health
risk from such bacteria in these occurrences needs to be continuously monitored, while, on the other hand,
mineral waters deserve special attention in the future from the aspect of screening for biotechnologically
relevant enzymes.
PB  - Kragujevac : Institute for Information Technologies
C3  - 2nd International Conference on Chemo and Bioinformatics
T1  - Bacteria in drinking and bathing mineral waters of Serbia with polymer-degrading potential
EP  - 195
SP  - 191
DO  - 10.46793/ICCBI23.091C
ER  - 

@conference{
author = "Ćirić, Milica and Šaraba, Vladimir and Budin, Clémence and Boer de, Tjalf and Milovanović, Jelena and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Three mineral water occurrences, captured by wells with a depth of 6.5-442.5 m and used for drinking
and bathing purposes, were sampled and cultivated under conditions favouring the growth of anaerobic,
microaerophilic or CO2 bacteria, in order to capture predominantly anaerobic portion of the bacteriome, which is
dominant in water and soils. Cultivated bacteria were identified by next-generation 16S sequencing and their
biotechnological potential in plastics and lignocellulose degradation was explored. Most abundant genera
detected in examined samples mainly belong to facultative anaerobes that are common representatives of water
and soil environments. In total, 17 genera were detected with a relative abundance over 1% in all three samples,
including Aeromonas, Exiguobacterium, Comamonas and Acinetobacter. Half of the screened isolates demonstrated
growth on at least one plastic or lignocellulosic polymer, with one isolate demonstrating growth on all tested
substrates, one demonstrating carboxymethyl cellulose- and one arabinoxylan-degrading ability. Some of the
representatives of genera identified with high relative abundance in mineral water samples, such as Aeromonas,
Klebsiella, Escherichia, Salmonella, Enterobacter, Pseudomonas and Staphylococcus, have been previously documented
to have pathogenic potential. Due to the use of investigated mineral waters for drinking and bathing, the health
risk from such bacteria in these occurrences needs to be continuously monitored, while, on the other hand,
mineral waters deserve special attention in the future from the aspect of screening for biotechnologically
relevant enzymes.",
publisher = "Kragujevac : Institute for Information Technologies",
journal = "2nd International Conference on Chemo and Bioinformatics",
title = "Bacteria in drinking and bathing mineral waters of Serbia with polymer-degrading potential",
pages = "195-191",
doi = "10.46793/ICCBI23.091C"
}

Ćirić, M., Šaraba, V., Budin, C., Boer de, T., Milovanović, J.,& Nikodinović-Runić, J.. (2023). Bacteria in drinking and bathing mineral waters of Serbia with polymer-degrading potential. in 2nd International Conference on Chemo and Bioinformatics
Kragujevac : Institute for Information Technologies., 191-195.
https://doi.org/10.46793/ICCBI23.091C

Ćirić M, Šaraba V, Budin C, Boer de T, Milovanović J, Nikodinović-Runić J. Bacteria in drinking and bathing mineral waters of Serbia with polymer-degrading potential. in 2nd International Conference on Chemo and Bioinformatics. 2023;:191-195.
doi:10.46793/ICCBI23.091C .

Ćirić, Milica, Šaraba, Vladimir, Budin, Clémence, Boer de, Tjalf, Milovanović, Jelena, Nikodinović-Runić, Jasmina, "Bacteria in drinking and bathing mineral waters of Serbia with polymer-degrading potential" in 2nd International Conference on Chemo and Bioinformatics (2023):191-195,
https://doi.org/10.46793/ICCBI23.091C . .

TY  - CONF
AU  - Lazić, Jelena
AU  - Milovanović, Jelena
AU  - Anejević, Tina
AU  - Ašanin, Darko
AU  - Ilić-Tomić, Tatjana
AU  - Glišić, Biljana
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://afea.eventsair.com/10th-conference-of-mikrobiokosmos/abstract-book
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2235
AB  - Prodigiosin (PG, Fig. 1a) is a biologically active
pyrrolylpyrromethene alkaloid whose structure was
first confirmed in 1962 [1]. PG is commonly
produced by Gram-negative bacteria, such as
Serratia spp. and has an eco-physiological role [2].
Its biological activities were extensively researched,
and numerous pharmacological properties were
established, including anticancer and
immunosuppressive However, high cost of extraction and purification
still represent the bottleneck in the microbial
production of PG. Meat and fish processing wastes have high potential as raw materials for conversion
into useful products of higher value. In this study,
meat offcuts were assessed as the sole nutrient for
the fermentative production of PG from S.
marcescens. Using this substrate lowered the
cultivation medium cost and shortened the
fermentation time to 12 h, while allowing a
satisfying PG yield of 83.1 mg/L. The isolated PG
was used in one-step reactions with CuCl₂ or ZnCl₂
in terc-BuOH at 25 °C. The obtained [Cu(PG)Cl] (Fig.
1b) and [Zn(PG)₂] (Fig. 1c) complexes were
characterized by UV-Vis and IR spectroscopy and
their bioactivity potential was assessed.
Antimicrobial activity was assessed in a disc assay
against 4 human pathogens: Escherichia coli NCTC
9001, Pseudomonas aeruginosa ATCC 10332,
Staphylococcus aureus NCTC 6571, Candida
albicans ATCC 10231, but no effect was observed
for the tested concentrations of 200 μg per disc and
lower. However, the anticancer potential of the new
derivatives is promising and the bovine serum
albumin (BSA) binding study revealed that
complexes bind to BSA tightly and reversibly [4].
C3  - 10th Conference of Mikrobiokosmos
T1  - Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II)
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2235
ER  - 

@conference{
author = "Lazić, Jelena and Milovanović, Jelena and Anejević, Tina and Ašanin, Darko and Ilić-Tomić, Tatjana and Glišić, Biljana and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Prodigiosin (PG, Fig. 1a) is a biologically active
pyrrolylpyrromethene alkaloid whose structure was
first confirmed in 1962 [1]. PG is commonly
produced by Gram-negative bacteria, such as
Serratia spp. and has an eco-physiological role [2].
Its biological activities were extensively researched,
and numerous pharmacological properties were
established, including anticancer and
immunosuppressive However, high cost of extraction and purification
still represent the bottleneck in the microbial
production of PG. Meat and fish processing wastes have high potential as raw materials for conversion
into useful products of higher value. In this study,
meat offcuts were assessed as the sole nutrient for
the fermentative production of PG from S.
marcescens. Using this substrate lowered the
cultivation medium cost and shortened the
fermentation time to 12 h, while allowing a
satisfying PG yield of 83.1 mg/L. The isolated PG
was used in one-step reactions with CuCl₂ or ZnCl₂
in terc-BuOH at 25 °C. The obtained [Cu(PG)Cl] (Fig.
1b) and [Zn(PG)₂] (Fig. 1c) complexes were
characterized by UV-Vis and IR spectroscopy and
their bioactivity potential was assessed.
Antimicrobial activity was assessed in a disc assay
against 4 human pathogens: Escherichia coli NCTC
9001, Pseudomonas aeruginosa ATCC 10332,
Staphylococcus aureus NCTC 6571, Candida
albicans ATCC 10231, but no effect was observed
for the tested concentrations of 200 μg per disc and
lower. However, the anticancer potential of the new
derivatives is promising and the bovine serum
albumin (BSA) binding study revealed that
complexes bind to BSA tightly and reversibly [4].",
journal = "10th Conference of Mikrobiokosmos",
title = "Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II)",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2235"
}

Lazić, J., Milovanović, J., Anejević, T., Ašanin, D., Ilić-Tomić, T., Glišić, B.,& Nikodinović-Runić, J.. (2023). Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II). in 10th Conference of Mikrobiokosmos.
https://hdl.handle.net/21.15107/rcub_imagine_2235

Lazić J, Milovanović J, Anejević T, Ašanin D, Ilić-Tomić T, Glišić B, Nikodinović-Runić J. Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II). in 10th Conference of Mikrobiokosmos. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2235 .

Lazić, Jelena, Milovanović, Jelena, Anejević, Tina, Ašanin, Darko, Ilić-Tomić, Tatjana, Glišić, Biljana, Nikodinović-Runić, Jasmina, "Biotechnological production of bacterial pigment prodigiosin and bioactive properties of its metal complexes with Cu(II) and Zn(II)" in 10th Conference of Mikrobiokosmos (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2235 .

TY  - JOUR
AU  - Šaraba, Vladimir
AU  - Milovanović, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Budin, Clémence
AU  - de Boer, Tjalf
AU  - Ćirić, Milica
PY  - 2023
UR  - https://doi.org/10.1007/s00248-023-02278-8
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2062
AB  - The selected brackish groundwater occurrences in the geotectonic regions of Inner Dinarides of western Serbia (Obrenovačka Banja) and Serbian crystalline core (Lomnički Kiseljak and Velika Vrbnica) were sampled for isolation and identification of plastic- and lignocellulose-degrading bacteria, as well as for the assessment of their enzymatic potential. The examined occurrences belong to the cold and warm (subthermal), weakly alkaline, neutral, and weakly acidic groundwater, and their genetic types are HCO3–Na + K and HCO3–Ca, Mg. The most abundant genera identified by next-generation 16S sequencing of cultivated groundwater samples belong to Aeromonas and Exiguobacterium. Of isolates screened on plastic and lignocellulosic substrates, 85.3% demonstrated growth and/or degrading activity on at least one tested substrate, with 27.8% isolates degrading plastic substrate Impranil® DLN-SD (SD), 1.9% plastic substrate bis(2-hydroxyethyl)terephthalate, and 5.6% carboxymethyl cellulose (CMC). Isolates degrading SD that were identified by 16S rDNA sequencing belonged to genera Stenotrophomonas, Flavobacterium, Pantoea, Enterobacter, Pseudomonas, Serratia, Acinetobacter, and Proteus, while isolates degrading CMC belonged to genera Rhizobium and Shewanella. All investigated brackish groundwaters harbor bacteria with potential in degradation of plastics or cellulose. Taking into account that microplastics contamination of groundwater resources is becoming a significant problem, the finding of plastic-degrading bacteria may have potential in bioremediation treatments of polluted groundwater. Subterranean ecosystems, which are largely untapped resources of biotechnologically relevant enzymes, are not traditionally considered the environment of choice for screening for plastic- and cellulose-degrading bacteria and therefore deserve a special attention from this aspect.
T2  - Microbial Ecology
T2  - Microbial EcologyMicrob Ecol
T1  - Brackish Groundwaters Contain Plastic- and Cellulose-Degrading Bacteria
DO  - 10.1007/s00248-023-02278-8
ER  - 

@article{
author = "Šaraba, Vladimir and Milovanović, Jelena and Nikodinović-Runić, Jasmina and Budin, Clémence and de Boer, Tjalf and Ćirić, Milica",
year = "2023",
abstract = "The selected brackish groundwater occurrences in the geotectonic regions of Inner Dinarides of western Serbia (Obrenovačka Banja) and Serbian crystalline core (Lomnički Kiseljak and Velika Vrbnica) were sampled for isolation and identification of plastic- and lignocellulose-degrading bacteria, as well as for the assessment of their enzymatic potential. The examined occurrences belong to the cold and warm (subthermal), weakly alkaline, neutral, and weakly acidic groundwater, and their genetic types are HCO3–Na + K and HCO3–Ca, Mg. The most abundant genera identified by next-generation 16S sequencing of cultivated groundwater samples belong to Aeromonas and Exiguobacterium. Of isolates screened on plastic and lignocellulosic substrates, 85.3% demonstrated growth and/or degrading activity on at least one tested substrate, with 27.8% isolates degrading plastic substrate Impranil® DLN-SD (SD), 1.9% plastic substrate bis(2-hydroxyethyl)terephthalate, and 5.6% carboxymethyl cellulose (CMC). Isolates degrading SD that were identified by 16S rDNA sequencing belonged to genera Stenotrophomonas, Flavobacterium, Pantoea, Enterobacter, Pseudomonas, Serratia, Acinetobacter, and Proteus, while isolates degrading CMC belonged to genera Rhizobium and Shewanella. All investigated brackish groundwaters harbor bacteria with potential in degradation of plastics or cellulose. Taking into account that microplastics contamination of groundwater resources is becoming a significant problem, the finding of plastic-degrading bacteria may have potential in bioremediation treatments of polluted groundwater. Subterranean ecosystems, which are largely untapped resources of biotechnologically relevant enzymes, are not traditionally considered the environment of choice for screening for plastic- and cellulose-degrading bacteria and therefore deserve a special attention from this aspect.",
journal = "Microbial Ecology, Microbial EcologyMicrob Ecol",
title = "Brackish Groundwaters Contain Plastic- and Cellulose-Degrading Bacteria",
doi = "10.1007/s00248-023-02278-8"
}

Šaraba, V., Milovanović, J., Nikodinović-Runić, J., Budin, C., de Boer, T.,& Ćirić, M.. (2023). Brackish Groundwaters Contain Plastic- and Cellulose-Degrading Bacteria. in Microbial Ecology.
https://doi.org/10.1007/s00248-023-02278-8

Šaraba V, Milovanović J, Nikodinović-Runić J, Budin C, de Boer T, Ćirić M. Brackish Groundwaters Contain Plastic- and Cellulose-Degrading Bacteria. in Microbial Ecology. 2023;.
doi:10.1007/s00248-023-02278-8 .

Šaraba, Vladimir, Milovanović, Jelena, Nikodinović-Runić, Jasmina, Budin, Clémence, de Boer, Tjalf, Ćirić, Milica, "Brackish Groundwaters Contain Plastic- and Cellulose-Degrading Bacteria" in Microbial Ecology (2023),
https://doi.org/10.1007/s00248-023-02278-8 . .

TY  - JOUR
AU  - Pekmezović, Marina
AU  - Krusić, Melina Kalagasidis
AU  - Malagurski, Ivana
AU  - Milovanović, Jelena
AU  - Stepien, Karolina
AU  - Guzik, Maciej
AU  - Charifou, Romina
AU  - Babu, Ramesh
AU  - O'Connor, Kevin
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1440
AB  - Novel biodegradable and biocompatible formulations of "old" but "gold" drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (similar to 50 mu m). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.
PB  - MDPI, Basel
T2  - Antibiotics-Basel
T1  - Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency
IS  - 6
VL  - 10
DO  - 10.3390/antibiotics10060737
ER  - 

@article{
author = "Pekmezović, Marina and Krusić, Melina Kalagasidis and Malagurski, Ivana and Milovanović, Jelena and Stepien, Karolina and Guzik, Maciej and Charifou, Romina and Babu, Ramesh and O'Connor, Kevin and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "Novel biodegradable and biocompatible formulations of "old" but "gold" drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (similar to 50 mu m). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.",
publisher = "MDPI, Basel",
journal = "Antibiotics-Basel",
title = "Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency",
number = "6",
volume = "10",
doi = "10.3390/antibiotics10060737"
}

Pekmezović, M., Krusić, M. K., Malagurski, I., Milovanović, J., Stepien, K., Guzik, M., Charifou, R., Babu, R., O'Connor, K.,& Nikodinović-Runić, J.. (2021). Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency. in Antibiotics-Basel
MDPI, Basel., 10(6).
https://doi.org/10.3390/antibiotics10060737

Pekmezović M, Krusić MK, Malagurski I, Milovanović J, Stepien K, Guzik M, Charifou R, Babu R, O'Connor K, Nikodinović-Runić J. Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency. in Antibiotics-Basel. 2021;10(6).
doi:10.3390/antibiotics10060737 .

Pekmezović, Marina, Krusić, Melina Kalagasidis, Malagurski, Ivana, Milovanović, Jelena, Stepien, Karolina, Guzik, Maciej, Charifou, Romina, Babu, Ramesh, O'Connor, Kevin, Nikodinović-Runić, Jasmina, "Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency" in Antibiotics-Basel, 10, no. 6 (2021),
https://doi.org/10.3390/antibiotics10060737 . .

TY  - JOUR
AU  - Milovanović, Jelena
AU  - Gunduz, Miyase Gozde
AU  - Zerva, Anastasia
AU  - Petković, Milos
AU  - Beskoski, Vladimir
AU  - Thomaidis, Nikolaos S.
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1442
AB  - We describe herein the synthesis and laccase mediated oxidation of six novel 1,4-dihydropyridine (DHP)-based hexahydroquinolines (DHP1-DHP3) and decahydroacridines (DHP4-DHP6). We employed different laccase enzymes with varying redox potential to convert DHP1-DHP3 and DHP4-DHP6 to the corresponding pyridine-containing tetrahydroquinoline and octahydroacridine derivatives, respectively. Intensively coloured products were detected in all biocatalytic reactions using laccase from Trametes versicolor (TvLacc), possibly due to the presence of conjugated chromophores formed in products after oxidation. The NMR assessment confirmed that the oxidation product of DHP1 was its corresponding pyridine-bearing tetrahydroquinoline derivative. Laccase from Bacillus subtillis (BacillusLacc) was the most efficient enzyme for this group of substrates using HPLC assessment. Overall, it could be concluded that DHP2 and DHP5, bearing catecholic structures, were easily oxidized by all tested laccases, while DHP3 and DHP6 containing electron-withdrawing nitro-groups are not readily oxidized by laccases. DHP4 with decahydroacridine moiety consisting of three condensed six-membered rings that contribute not only to the volume but also to the higher redox potential of the substrate rendered this compound not to be biotransformed with any of the mentioned enzymes. Overall, we showed that multiple analytical approaches are needed in order to assess biocatalytical reactions.
PB  - MDPI, Basel
T2  - Catalysts
T1  - Synthesis and Laccase-Mediated Oxidation of New Condensed 1,4-Dihydropyridine Derivatives
IS  - 6
VL  - 11
DO  - 10.3390/catal11060727
ER  - 

@article{
author = "Milovanović, Jelena and Gunduz, Miyase Gozde and Zerva, Anastasia and Petković, Milos and Beskoski, Vladimir and Thomaidis, Nikolaos S. and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "We describe herein the synthesis and laccase mediated oxidation of six novel 1,4-dihydropyridine (DHP)-based hexahydroquinolines (DHP1-DHP3) and decahydroacridines (DHP4-DHP6). We employed different laccase enzymes with varying redox potential to convert DHP1-DHP3 and DHP4-DHP6 to the corresponding pyridine-containing tetrahydroquinoline and octahydroacridine derivatives, respectively. Intensively coloured products were detected in all biocatalytic reactions using laccase from Trametes versicolor (TvLacc), possibly due to the presence of conjugated chromophores formed in products after oxidation. The NMR assessment confirmed that the oxidation product of DHP1 was its corresponding pyridine-bearing tetrahydroquinoline derivative. Laccase from Bacillus subtillis (BacillusLacc) was the most efficient enzyme for this group of substrates using HPLC assessment. Overall, it could be concluded that DHP2 and DHP5, bearing catecholic structures, were easily oxidized by all tested laccases, while DHP3 and DHP6 containing electron-withdrawing nitro-groups are not readily oxidized by laccases. DHP4 with decahydroacridine moiety consisting of three condensed six-membered rings that contribute not only to the volume but also to the higher redox potential of the substrate rendered this compound not to be biotransformed with any of the mentioned enzymes. Overall, we showed that multiple analytical approaches are needed in order to assess biocatalytical reactions.",
publisher = "MDPI, Basel",
journal = "Catalysts",
title = "Synthesis and Laccase-Mediated Oxidation of New Condensed 1,4-Dihydropyridine Derivatives",
number = "6",
volume = "11",
doi = "10.3390/catal11060727"
}

Milovanović, J., Gunduz, M. G., Zerva, A., Petković, M., Beskoski, V., Thomaidis, N. S., Topakas, E.,& Nikodinović-Runić, J.. (2021). Synthesis and Laccase-Mediated Oxidation of New Condensed 1,4-Dihydropyridine Derivatives. in Catalysts
MDPI, Basel., 11(6).
https://doi.org/10.3390/catal11060727

Milovanović J, Gunduz MG, Zerva A, Petković M, Beskoski V, Thomaidis NS, Topakas E, Nikodinović-Runić J. Synthesis and Laccase-Mediated Oxidation of New Condensed 1,4-Dihydropyridine Derivatives. in Catalysts. 2021;11(6).
doi:10.3390/catal11060727 .

Milovanović, Jelena, Gunduz, Miyase Gozde, Zerva, Anastasia, Petković, Milos, Beskoski, Vladimir, Thomaidis, Nikolaos S., Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Synthesis and Laccase-Mediated Oxidation of New Condensed 1,4-Dihydropyridine Derivatives" in Catalysts, 11, no. 6 (2021),
https://doi.org/10.3390/catal11060727 . .

TY  - JOUR
AU  - Jeremić, Sanja
AU  - Milovanović, Jelena
AU  - Mojicević, Marija
AU  - Škaro Bogojević, Sanja
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1343
AB  - Plastic pollution is now considered one of the largest environmental threats facing humans and animals globally. Development of bioplastic materials may offer part of the solution as bioplastics include both nondegradable and biodegradable materials with both being important for sustainability. Bioplastic materials are currently being designed to encompass minimal carbon footprint, high recycling value and complete biodegradability. This review examines recent developments and trends in the field of bioplastic materials. A range of the most utilized bioplastic materials is presented (poly(lactic acid) (PLA), polyhydroxyalkanoate (PHA), starch, cellulose, bio-based poly(butylene succinate) (bio-PBS) and bio-polyethylene (bio-PE)) including their production, application and degradation options.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Understanding bioplastic materials - Current state and trends
EP  - 1538
IS  - 12
SP  - 1507
VL  - 85
DO  - 10.2298/JSC200720051J
ER  - 

@article{
author = "Jeremić, Sanja and Milovanović, Jelena and Mojicević, Marija and Škaro Bogojević, Sanja and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "Plastic pollution is now considered one of the largest environmental threats facing humans and animals globally. Development of bioplastic materials may offer part of the solution as bioplastics include both nondegradable and biodegradable materials with both being important for sustainability. Bioplastic materials are currently being designed to encompass minimal carbon footprint, high recycling value and complete biodegradability. This review examines recent developments and trends in the field of bioplastic materials. A range of the most utilized bioplastic materials is presented (poly(lactic acid) (PLA), polyhydroxyalkanoate (PHA), starch, cellulose, bio-based poly(butylene succinate) (bio-PBS) and bio-polyethylene (bio-PE)) including their production, application and degradation options.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Understanding bioplastic materials - Current state and trends",
pages = "1538-1507",
number = "12",
volume = "85",
doi = "10.2298/JSC200720051J"
}

Jeremić, S., Milovanović, J., Mojicević, M., Škaro Bogojević, S.,& Nikodinović-Runić, J.. (2020). Understanding bioplastic materials - Current state and trends. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 85(12), 1507-1538.
https://doi.org/10.2298/JSC200720051J

Jeremić S, Milovanović J, Mojicević M, Škaro Bogojević S, Nikodinović-Runić J. Understanding bioplastic materials - Current state and trends. in Journal of the Serbian Chemical Society. 2020;85(12):1507-1538.
doi:10.2298/JSC200720051J .

Jeremić, Sanja, Milovanović, Jelena, Mojicević, Marija, Škaro Bogojević, Sanja, Nikodinović-Runić, Jasmina, "Understanding bioplastic materials - Current state and trends" in Journal of the Serbian Chemical Society, 85, no. 12 (2020):1507-1538,
https://doi.org/10.2298/JSC200720051J . .

TY  - JOUR
AU  - Jaksić, Jovana
AU  - Ostojić, Sanja
AU  - Micić, Darko
AU  - Vujosević, Zorana Tokic
AU  - Milovanović, Jelena
AU  - Karkalić, Radovan
AU  - O'Connor, Kevin
AU  - Kenny, Shane T.
AU  - Casey, William
AU  - Nikodinović-Runić, Jasmina
AU  - Maslak, Veselin
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1399
AB  - In the present work, we describe the chemical synthesis of 3-HFAs as prominent derivatives of fatty acids and assess if they could be applied as phase change materials (PCM). In addition, 3-HFAs were obtained by depolymerization of a bacterial biopolymeric material, polyhydroxyalkanoate. Thermal properties of 3-hydoxyoctanoic, decanoic, and dodecanoic acids are reported for the first time. These materials showed the potential to be applied as PCM in temperature range from 33 degrees C to 66 degrees C. In order to expand the temperature range for application of 3-HFAs as PCM, eutectic mass ratios of three kinds of binary mixtures of 3-HFAs were calculated, and their properties were predicted using the Schroder-van Laar equation. Thermal properties of these mixtures were validated by differential scanning calorimetry (DSC) analysis. These results showed that eutectics considerably expanded the scope of applications of 3-HFAs as PCMs. 3-HFAs originating from biotechnologically obtained polyhydroxyalkanoates also showed potential to be applied in development of PCMs.
PB  - Hoboken : Wiley
T2  - International Journal of Energy Research
T1  - Thermal properties of 3-hydroxy fatty acids and their binary mixtures as phase change energy storage materials
EP  - 1302
IS  - 2
SP  - 1294
VL  - 44
DO  - 10.1002/er.4934
ER  - 

@article{
author = "Jaksić, Jovana and Ostojić, Sanja and Micić, Darko and Vujosević, Zorana Tokic and Milovanović, Jelena and Karkalić, Radovan and O'Connor, Kevin and Kenny, Shane T. and Casey, William and Nikodinović-Runić, Jasmina and Maslak, Veselin",
year = "2020",
abstract = "In the present work, we describe the chemical synthesis of 3-HFAs as prominent derivatives of fatty acids and assess if they could be applied as phase change materials (PCM). In addition, 3-HFAs were obtained by depolymerization of a bacterial biopolymeric material, polyhydroxyalkanoate. Thermal properties of 3-hydoxyoctanoic, decanoic, and dodecanoic acids are reported for the first time. These materials showed the potential to be applied as PCM in temperature range from 33 degrees C to 66 degrees C. In order to expand the temperature range for application of 3-HFAs as PCM, eutectic mass ratios of three kinds of binary mixtures of 3-HFAs were calculated, and their properties were predicted using the Schroder-van Laar equation. Thermal properties of these mixtures were validated by differential scanning calorimetry (DSC) analysis. These results showed that eutectics considerably expanded the scope of applications of 3-HFAs as PCMs. 3-HFAs originating from biotechnologically obtained polyhydroxyalkanoates also showed potential to be applied in development of PCMs.",
publisher = "Hoboken : Wiley",
journal = "International Journal of Energy Research",
title = "Thermal properties of 3-hydroxy fatty acids and their binary mixtures as phase change energy storage materials",
pages = "1302-1294",
number = "2",
volume = "44",
doi = "10.1002/er.4934"
}

Jaksić, J., Ostojić, S., Micić, D., Vujosević, Z. T., Milovanović, J., Karkalić, R., O'Connor, K., Kenny, S. T., Casey, W., Nikodinović-Runić, J.,& Maslak, V.. (2020). Thermal properties of 3-hydroxy fatty acids and their binary mixtures as phase change energy storage materials. in International Journal of Energy Research
Hoboken : Wiley., 44(2), 1294-1302.
https://doi.org/10.1002/er.4934

Jaksić J, Ostojić S, Micić D, Vujosević ZT, Milovanović J, Karkalić R, O'Connor K, Kenny ST, Casey W, Nikodinović-Runić J, Maslak V. Thermal properties of 3-hydroxy fatty acids and their binary mixtures as phase change energy storage materials. in International Journal of Energy Research. 2020;44(2):1294-1302.
doi:10.1002/er.4934 .

Jaksić, Jovana, Ostojić, Sanja, Micić, Darko, Vujosević, Zorana Tokic, Milovanović, Jelena, Karkalić, Radovan, O'Connor, Kevin, Kenny, Shane T., Casey, William, Nikodinović-Runić, Jasmina, Maslak, Veselin, "Thermal properties of 3-hydroxy fatty acids and their binary mixtures as phase change energy storage materials" in International Journal of Energy Research, 44, no. 2 (2020):1294-1302,
https://doi.org/10.1002/er.4934 . .

TY  - JOUR
AU  - Cerrone, Federico
AU  - Milovanović, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Walsh, Meg
AU  - Kenny, Shane T.
AU  - Babu, Ramesh
AU  - O'Connor, Kevin 
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1199
AB  - A sodium alkyl disulfate mixture (SADM) synthesised from microbially produced 3-hydroxy fatty acids methyl esters (HFAMEs), showed 13-fold surface tension decrease when compared with the reference surfactant sodium dodecyl sulfate (SDS). Polyhydroxyalkanoates, accumulated by bacteria intracellularly when supplied with a mixture of fatty acids derived from hydrolysed rapeseed oil, were isolated, depolymerised and methylated to produce HFAMEs in very high yield (90%). A sequential chemical reduction and sulfation of the HFAMEs produced the sodium alkyl disulfates in high yields ( gt  65%). SADM performs also 1.3-times better than dodecyl (1,3) disulfate, in surface tension tests. SADM shows also the formation of a specific critical micelle concentration (CMC) at a concentration 21-fold lower than SDS. The wettability of the SADM mixture is similar to SDS but the foaming volume of SADM is 1.5-fold higher. The foam is also more stable with its volume decreasing 3 times slower over time compared to SDS at their respective CMC values. Established sulfation technologies in chemical manufacturing could use the 3-hydroxy fatty acids methyl esters moiety (3-HFAME) given its origin from rapeseed oil and the extra OH residue on 3-position in the molecule, which affords the opportunity to produce disulfate surfactants with a proven superior performance to monosulphated surfactants. Thus, not only addressing environmental issues by avoiding threats of deforestation and monocultivation associated with palm oil use but also achieve a higher performance with lower use of surfactants.
PB  - Elsevier, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Novel sodium alkyl-1,3-disulfates, anionic biosurfactants produced from microbial polyesters
VL  - 182
DO  - 10.1016/j.colsurfb.2019.06.062
ER  - 

@article{
author = "Cerrone, Federico and Milovanović, Jelena and Nikodinović-Runić, Jasmina and Walsh, Meg and Kenny, Shane T. and Babu, Ramesh and O'Connor, Kevin ",
year = "2019",
abstract = "A sodium alkyl disulfate mixture (SADM) synthesised from microbially produced 3-hydroxy fatty acids methyl esters (HFAMEs), showed 13-fold surface tension decrease when compared with the reference surfactant sodium dodecyl sulfate (SDS). Polyhydroxyalkanoates, accumulated by bacteria intracellularly when supplied with a mixture of fatty acids derived from hydrolysed rapeseed oil, were isolated, depolymerised and methylated to produce HFAMEs in very high yield (90%). A sequential chemical reduction and sulfation of the HFAMEs produced the sodium alkyl disulfates in high yields ( gt  65%). SADM performs also 1.3-times better than dodecyl (1,3) disulfate, in surface tension tests. SADM shows also the formation of a specific critical micelle concentration (CMC) at a concentration 21-fold lower than SDS. The wettability of the SADM mixture is similar to SDS but the foaming volume of SADM is 1.5-fold higher. The foam is also more stable with its volume decreasing 3 times slower over time compared to SDS at their respective CMC values. Established sulfation technologies in chemical manufacturing could use the 3-hydroxy fatty acids methyl esters moiety (3-HFAME) given its origin from rapeseed oil and the extra OH residue on 3-position in the molecule, which affords the opportunity to produce disulfate surfactants with a proven superior performance to monosulphated surfactants. Thus, not only addressing environmental issues by avoiding threats of deforestation and monocultivation associated with palm oil use but also achieve a higher performance with lower use of surfactants.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Novel sodium alkyl-1,3-disulfates, anionic biosurfactants produced from microbial polyesters",
volume = "182",
doi = "10.1016/j.colsurfb.2019.06.062"
}

Cerrone, F., Milovanović, J., Nikodinović-Runić, J., Walsh, M., Kenny, S. T., Babu, R.,& O'Connor, K.. (2019). Novel sodium alkyl-1,3-disulfates, anionic biosurfactants produced from microbial polyesters. in Colloids and Surfaces B-Biointerfaces
Elsevier, Amsterdam., 182.
https://doi.org/10.1016/j.colsurfb.2019.06.062

Cerrone F, Milovanović J, Nikodinović-Runić J, Walsh M, Kenny ST, Babu R, O'Connor K. Novel sodium alkyl-1,3-disulfates, anionic biosurfactants produced from microbial polyesters. in Colloids and Surfaces B-Biointerfaces. 2019;182.
doi:10.1016/j.colsurfb.2019.06.062 .

Cerrone, Federico, Milovanović, Jelena, Nikodinović-Runić, Jasmina, Walsh, Meg, Kenny, Shane T., Babu, Ramesh, O'Connor, Kevin , "Novel sodium alkyl-1,3-disulfates, anionic biosurfactants produced from microbial polyesters" in Colloids and Surfaces B-Biointerfaces, 182 (2019),
https://doi.org/10.1016/j.colsurfb.2019.06.062 . .

TY  - JOUR
AU  - Jovanović, Milos
AU  - Milovanović, Jelena
AU  - O'Connor, Kevin
AU  - Blagojević, Stevan
AU  - Begović, Biljana 
AU  - Lukić, Vera
AU  - Nikodinović-Runić, Jasmina
AU  - Savić, Vladimir
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1258
AB  - Rhamnolipids are biodegradable low toxic biosurfactants which exert antimicrobial and anti-biofilm properties. They have attracted much attention recently due to potential applications in areas of bioremediation, therapeutics, cosmetics and agriculture, however, the full potential of these versatile molecules is yet to be explored. Based on the facts that many naturally occurring lipopeptides are potent antimicrobials, our study aimed to explore the potential of replacing rhamnose in rhamnolipids with amino acids thus creating lipopeptides that would mimic or enhance properties of the parent molecule. This would allow not only for more economical and greener production but also, due to the availability of structurally different amino acids, facile manipulation of physico-chemical and biological properties. Our synthetic efforts produced a library of 43 lipopeptides revealing biologically more potent molecules. The structural changes significantly increased, in particular, anti-biofilm properties against Candida albicans, although surface activity of the parent molecule was almost completely abolished. Our findings show that the most active compounds are leucine derivatives of 3-hydroxy acids containing benzylic ester functionality. The SAR study demonstrated a further increase in activity with aliphatic chain elongation. The most promising lipopeptides 15, 23 and 36 at 12.5 mu g/mL concentration allowed only 14.3%, 5.1% and 11.2% of biofilm formation, respectively after 24 h. These compounds inhibit biofilm formation by preventing adhesion of C. albicans to abiotic and biotic surfaces.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Bioorganic Chemistry
T1  - Rhamnolipid inspired lipopeptides effective in preventing adhesion and biofilm formation of Candida albicans
EP  - 217
SP  - 209
VL  - 87
DO  - 10.1016/j.bioorg.2019.03.023
ER  - 

@article{
author = "Jovanović, Milos and Milovanović, Jelena and O'Connor, Kevin and Blagojević, Stevan and Begović, Biljana  and Lukić, Vera and Nikodinović-Runić, Jasmina and Savić, Vladimir",
year = "2019",
abstract = "Rhamnolipids are biodegradable low toxic biosurfactants which exert antimicrobial and anti-biofilm properties. They have attracted much attention recently due to potential applications in areas of bioremediation, therapeutics, cosmetics and agriculture, however, the full potential of these versatile molecules is yet to be explored. Based on the facts that many naturally occurring lipopeptides are potent antimicrobials, our study aimed to explore the potential of replacing rhamnose in rhamnolipids with amino acids thus creating lipopeptides that would mimic or enhance properties of the parent molecule. This would allow not only for more economical and greener production but also, due to the availability of structurally different amino acids, facile manipulation of physico-chemical and biological properties. Our synthetic efforts produced a library of 43 lipopeptides revealing biologically more potent molecules. The structural changes significantly increased, in particular, anti-biofilm properties against Candida albicans, although surface activity of the parent molecule was almost completely abolished. Our findings show that the most active compounds are leucine derivatives of 3-hydroxy acids containing benzylic ester functionality. The SAR study demonstrated a further increase in activity with aliphatic chain elongation. The most promising lipopeptides 15, 23 and 36 at 12.5 mu g/mL concentration allowed only 14.3%, 5.1% and 11.2% of biofilm formation, respectively after 24 h. These compounds inhibit biofilm formation by preventing adhesion of C. albicans to abiotic and biotic surfaces.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Bioorganic Chemistry",
title = "Rhamnolipid inspired lipopeptides effective in preventing adhesion and biofilm formation of Candida albicans",
pages = "217-209",
volume = "87",
doi = "10.1016/j.bioorg.2019.03.023"
}

Jovanović, M., Milovanović, J., O'Connor, K., Blagojević, S., Begović, B., Lukić, V., Nikodinović-Runić, J.,& Savić, V.. (2019). Rhamnolipid inspired lipopeptides effective in preventing adhesion and biofilm formation of Candida albicans. in Bioorganic Chemistry
Academic Press Inc Elsevier Science, San Diego., 87, 209-217.
https://doi.org/10.1016/j.bioorg.2019.03.023

Jovanović M, Milovanović J, O'Connor K, Blagojević S, Begović B, Lukić V, Nikodinović-Runić J, Savić V. Rhamnolipid inspired lipopeptides effective in preventing adhesion and biofilm formation of Candida albicans. in Bioorganic Chemistry. 2019;87:209-217.
doi:10.1016/j.bioorg.2019.03.023 .

Jovanović, Milos, Milovanović, Jelena, O'Connor, Kevin, Blagojević, Stevan, Begović, Biljana , Lukić, Vera, Nikodinović-Runić, Jasmina, Savić, Vladimir, "Rhamnolipid inspired lipopeptides effective in preventing adhesion and biofilm formation of Candida albicans" in Bioorganic Chemistry, 87 (2019):209-217,
https://doi.org/10.1016/j.bioorg.2019.03.023 . .

TY  - JOUR
AU  - Spasić, Jelena
AU  - Mandić, Mina
AU  - Radivojević, Jelena
AU  - Jeremić, Sanja
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
AU  - Đokić, Lidija
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1134
AB  - Biocatalytic potential of Streptomyces strains isolated from the rhizosphere of plants and from mycorrhizosphere of fungi has been investigated. A total of 118 Streptomyces isolates were selected and functionally screened for 10 different biotechnologically important enzymatic activities: hydrolase (cellulase, cutinase, gelatinase, lipase, protease, polyhydroxyalkanoate (PHA) depolymerase), phenol oxidase and peroxidase (laccase, tyrosinase, and lignin peroxidase), and aminotransferase. Out of 118 tested Streptomyces spp., 90% showed at least one enzymatic activity. The most abundant were enzymes involved in the biomass degradation, as the production of cutinase, cellulase, and lignin peroxidase were detected in 31%, 40%, and 48% of the isolates, respectively. The improved specific activities of lipase (isolates BV315 and BV100) and tyrosinase (isolates BV87 and BV88) were shown in comparison with the industrially relevant activities of Pseudomonas strains. Plant rhizosphere soils were more prolific source of Streptomyces strains with biocatalytic potential in comparison with mycorrhizosphere soils. Overall, 284 enzyme activities among 118 Streptomyces isolates have been detected. This is the first comprehensive screening of Streptomyces isolates from rhizosphere and mycorrhizosphere soils for novel biocatalysts, showing that specific environmental habitats, such as rhizosphere soils, are treasure troves of Streptomyces with biocatalytic potential.
PB  - Wiley, Hoboken
T2  - Biotechnology and Applied Biochemistry
T1  - Biocatalytic potential of Streptomyces spp. isolates from rhizosphere of plants and mycorrhizosphere of fungi
EP  - 833
IS  - 6
SP  - 822
VL  - 65
DO  - 10.1002/bab.1664
ER  - 

@article{
author = "Spasić, Jelena and Mandić, Mina and Radivojević, Jelena and Jeremić, Sanja and Vasiljević, Branka and Nikodinović-Runić, Jasmina and Đokić, Lidija",
year = "2018",
abstract = "Biocatalytic potential of Streptomyces strains isolated from the rhizosphere of plants and from mycorrhizosphere of fungi has been investigated. A total of 118 Streptomyces isolates were selected and functionally screened for 10 different biotechnologically important enzymatic activities: hydrolase (cellulase, cutinase, gelatinase, lipase, protease, polyhydroxyalkanoate (PHA) depolymerase), phenol oxidase and peroxidase (laccase, tyrosinase, and lignin peroxidase), and aminotransferase. Out of 118 tested Streptomyces spp., 90% showed at least one enzymatic activity. The most abundant were enzymes involved in the biomass degradation, as the production of cutinase, cellulase, and lignin peroxidase were detected in 31%, 40%, and 48% of the isolates, respectively. The improved specific activities of lipase (isolates BV315 and BV100) and tyrosinase (isolates BV87 and BV88) were shown in comparison with the industrially relevant activities of Pseudomonas strains. Plant rhizosphere soils were more prolific source of Streptomyces strains with biocatalytic potential in comparison with mycorrhizosphere soils. Overall, 284 enzyme activities among 118 Streptomyces isolates have been detected. This is the first comprehensive screening of Streptomyces isolates from rhizosphere and mycorrhizosphere soils for novel biocatalysts, showing that specific environmental habitats, such as rhizosphere soils, are treasure troves of Streptomyces with biocatalytic potential.",
publisher = "Wiley, Hoboken",
journal = "Biotechnology and Applied Biochemistry",
title = "Biocatalytic potential of Streptomyces spp. isolates from rhizosphere of plants and mycorrhizosphere of fungi",
pages = "833-822",
number = "6",
volume = "65",
doi = "10.1002/bab.1664"
}

Spasić, J., Mandić, M., Radivojević, J., Jeremić, S., Vasiljević, B., Nikodinović-Runić, J.,& Đokić, L.. (2018). Biocatalytic potential of Streptomyces spp. isolates from rhizosphere of plants and mycorrhizosphere of fungi. in Biotechnology and Applied Biochemistry
Wiley, Hoboken., 65(6), 822-833.
https://doi.org/10.1002/bab.1664

Spasić J, Mandić M, Radivojević J, Jeremić S, Vasiljević B, Nikodinović-Runić J, Đokić L. Biocatalytic potential of Streptomyces spp. isolates from rhizosphere of plants and mycorrhizosphere of fungi. in Biotechnology and Applied Biochemistry. 2018;65(6):822-833.
doi:10.1002/bab.1664 .

Spasić, Jelena, Mandić, Mina, Radivojević, Jelena, Jeremić, Sanja, Vasiljević, Branka, Nikodinović-Runić, Jasmina, Đokić, Lidija, "Biocatalytic potential of Streptomyces spp. isolates from rhizosphere of plants and mycorrhizosphere of fungi" in Biotechnology and Applied Biochemistry, 65, no. 6 (2018):822-833,
https://doi.org/10.1002/bab.1664 . .

TY  - JOUR
AU  - Milovanović, Jelena
AU  - Škaro Bogojević, Sanja
AU  - Šenerović, Lidija
AU  - Vasiljević, Branka
AU  - Guzik, Maciej
AU  - Kenny, Shane T.
AU  - Maslak, Veselin
AU  - Nikodinović-Runić, Jasmina
AU  - O'Connor, Kevin 
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/974
AB  - A library of 18 different compounds was synthesized starting from (R)-3-hydroxyoctanoic acid which is derived from the bacterial polymer polyhydroxyalkanoate (PHA). Ten derivatives, including halo and unsaturated methyl and benzyl esters, were synthesized and characterized for the first time. Given that (R)-3-hydroxyalkanoic acids are known to have biological activity, the new compounds were evaluated for antimicrobial activity and in vitro antiproliferative effect with mammalian cell lines. The presence of the carboxylic group was essential for the antimicrobial activity, with minimal inhibitory concentrations against a panel of bacteria (Gram-positive and Gram-negative) and fungi (Candida albicans and Microsporum gypseum) in the range 2.8-7.0 mM and 0.1-6.3 mM, respectively. 3-Halogenated octanoic acids exhibited the ability to inhibit C. albicans hyphae formation. In addition, (R)-3-hydroxyoctanoic and (E)-oct-2-enoic acids inhibited quorum sensing-regulated pyocyanin production in the opportunistic pathogen Pseudomonas aeruginosa PAO1. Generally, derivatives did not inhibit mammalian cell proliferation even at 3-mM concentrations, while only (E)-oct-2-enoic and 3-oxooctanoic acid had IC50 values of 1.7 and 1.6 mM with the human lung fibroblast cell line.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds
EP  - 172
IS  - 1
SP  - 161
VL  - 100
DO  - 10.1007/s00253-015-6984-4
ER  - 

@article{
author = "Milovanović, Jelena and Škaro Bogojević, Sanja and Šenerović, Lidija and Vasiljević, Branka and Guzik, Maciej and Kenny, Shane T. and Maslak, Veselin and Nikodinović-Runić, Jasmina and O'Connor, Kevin ",
year = "2016",
abstract = "A library of 18 different compounds was synthesized starting from (R)-3-hydroxyoctanoic acid which is derived from the bacterial polymer polyhydroxyalkanoate (PHA). Ten derivatives, including halo and unsaturated methyl and benzyl esters, were synthesized and characterized for the first time. Given that (R)-3-hydroxyalkanoic acids are known to have biological activity, the new compounds were evaluated for antimicrobial activity and in vitro antiproliferative effect with mammalian cell lines. The presence of the carboxylic group was essential for the antimicrobial activity, with minimal inhibitory concentrations against a panel of bacteria (Gram-positive and Gram-negative) and fungi (Candida albicans and Microsporum gypseum) in the range 2.8-7.0 mM and 0.1-6.3 mM, respectively. 3-Halogenated octanoic acids exhibited the ability to inhibit C. albicans hyphae formation. In addition, (R)-3-hydroxyoctanoic and (E)-oct-2-enoic acids inhibited quorum sensing-regulated pyocyanin production in the opportunistic pathogen Pseudomonas aeruginosa PAO1. Generally, derivatives did not inhibit mammalian cell proliferation even at 3-mM concentrations, while only (E)-oct-2-enoic and 3-oxooctanoic acid had IC50 values of 1.7 and 1.6 mM with the human lung fibroblast cell line.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds",
pages = "172-161",
number = "1",
volume = "100",
doi = "10.1007/s00253-015-6984-4"
}

Milovanović, J., Škaro Bogojević, S., Šenerović, L., Vasiljević, B., Guzik, M., Kenny, S. T., Maslak, V., Nikodinović-Runić, J.,& O'Connor, K.. (2016). Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds. in Applied Microbiology and Biotechnology
Springer, New York., 100(1), 161-172.
https://doi.org/10.1007/s00253-015-6984-4

Milovanović J, Škaro Bogojević S, Šenerović L, Vasiljević B, Guzik M, Kenny ST, Maslak V, Nikodinović-Runić J, O'Connor K. Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds. in Applied Microbiology and Biotechnology. 2016;100(1):161-172.
doi:10.1007/s00253-015-6984-4 .

Milovanović, Jelena, Škaro Bogojević, Sanja, Šenerović, Lidija, Vasiljević, Branka, Guzik, Maciej, Kenny, Shane T., Maslak, Veselin, Nikodinović-Runić, Jasmina, O'Connor, Kevin , "Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds" in Applied Microbiology and Biotechnology, 100, no. 1 (2016):161-172,
https://doi.org/10.1007/s00253-015-6984-4 . .

TY  - JOUR
AU  - Szwej, Emilia
AU  - Devocelle, Marc
AU  - Kenny, Shane
AU  - Guzik, Maciej
AU  - O'Connor, Stephen
AU  - Nikodinović-Runić, Jasmina
AU  - Milovanović, Jelena
AU  - Maslak, Veselin
AU  - Byrne, Annete T.
AU  - Gallagher, William M.
AU  - Zulian, Qun Ren
AU  - Zinn, Manfred
AU  - O'Connor, Kevin 
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/877
AB  - Conjugation of DP18L peptide with (R)-3-hydroxydecanoic acid, derived from the biopolymer polyhydroxyalkanoate, enhances its anti-cancer activity (O'Connor et al., 2013. Biomaterials 34, 2710-2718). However, it is unknown if other (R)-3-hydroxyalkanoic acids (R3HA5) can enhance peptide activity, if chain length affects enhancement, and what effect R3HA5 have on peptide structure. Here we show that the degree of enhancement of peptide (DP18L) anti-cancer activity by R3HA5 is carbon chain length dependent. In all but one example the R3HA conjugated peptides were more active against cancer cells than the unconjugated peptides. However, R3HA5 with 9 and 10 carbons were most effective at improving DPI 8L activity. DPI 8L peptide variant DPI 7L, missing a hydrophobic amino acid (leucine residue 4) exhibited lower efficacy against MiaPaCa cells. Circular dichroism analysis showed DP17L had a lower alpha helix content and the conjugation of any R3HA ((R)-3-hydroxyhexanoic acid to (R)-3-hydroxydodecanoic acid) to DPI 7L returned the helix content back to levels of DPI 8L. However (R)-3-hydroxyhexanoic did not enhance the anti-cancer activity of DPI 7L and at least 7 carbons were needed in the R3HA to enhance activity of D17L. DP17L needs a longer chain R3HA to achieve the same activity as DP18L conjugated to an R3HA. As a first step to assess the synthetic potential of polyhydroxyalkanoate derived R3HA5, (R)-3-hydroxydecanoic acid was synthetically converted to (+/-)3-chlorodecanoic acid, which when conjugated to DP18L improved its antiproliferative activity against MiaPaCa cells.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Biotechnology
T1  - The chain length of biologically produced (R)-3-hydroxyalkanoic acid affects biological activity and structure of anti-cancer peptides
EP  - 12
SP  - 7
VL  - 204
DO  - 10.1016/j.jbiotec.2015.02.036
ER  - 

@article{
author = "Szwej, Emilia and Devocelle, Marc and Kenny, Shane and Guzik, Maciej and O'Connor, Stephen and Nikodinović-Runić, Jasmina and Milovanović, Jelena and Maslak, Veselin and Byrne, Annete T. and Gallagher, William M. and Zulian, Qun Ren and Zinn, Manfred and O'Connor, Kevin ",
year = "2015",
abstract = "Conjugation of DP18L peptide with (R)-3-hydroxydecanoic acid, derived from the biopolymer polyhydroxyalkanoate, enhances its anti-cancer activity (O'Connor et al., 2013. Biomaterials 34, 2710-2718). However, it is unknown if other (R)-3-hydroxyalkanoic acids (R3HA5) can enhance peptide activity, if chain length affects enhancement, and what effect R3HA5 have on peptide structure. Here we show that the degree of enhancement of peptide (DP18L) anti-cancer activity by R3HA5 is carbon chain length dependent. In all but one example the R3HA conjugated peptides were more active against cancer cells than the unconjugated peptides. However, R3HA5 with 9 and 10 carbons were most effective at improving DPI 8L activity. DPI 8L peptide variant DPI 7L, missing a hydrophobic amino acid (leucine residue 4) exhibited lower efficacy against MiaPaCa cells. Circular dichroism analysis showed DP17L had a lower alpha helix content and the conjugation of any R3HA ((R)-3-hydroxyhexanoic acid to (R)-3-hydroxydodecanoic acid) to DPI 7L returned the helix content back to levels of DPI 8L. However (R)-3-hydroxyhexanoic did not enhance the anti-cancer activity of DPI 7L and at least 7 carbons were needed in the R3HA to enhance activity of D17L. DP17L needs a longer chain R3HA to achieve the same activity as DP18L conjugated to an R3HA. As a first step to assess the synthetic potential of polyhydroxyalkanoate derived R3HA5, (R)-3-hydroxydecanoic acid was synthetically converted to (+/-)3-chlorodecanoic acid, which when conjugated to DP18L improved its antiproliferative activity against MiaPaCa cells.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Biotechnology",
title = "The chain length of biologically produced (R)-3-hydroxyalkanoic acid affects biological activity and structure of anti-cancer peptides",
pages = "12-7",
volume = "204",
doi = "10.1016/j.jbiotec.2015.02.036"
}

Szwej, E., Devocelle, M., Kenny, S., Guzik, M., O'Connor, S., Nikodinović-Runić, J., Milovanović, J., Maslak, V., Byrne, A. T., Gallagher, W. M., Zulian, Q. R., Zinn, M.,& O'Connor, K.. (2015). The chain length of biologically produced (R)-3-hydroxyalkanoic acid affects biological activity and structure of anti-cancer peptides. in Journal of Biotechnology
Elsevier Science Bv, Amsterdam., 204, 7-12.
https://doi.org/10.1016/j.jbiotec.2015.02.036

Szwej E, Devocelle M, Kenny S, Guzik M, O'Connor S, Nikodinović-Runić J, Milovanović J, Maslak V, Byrne AT, Gallagher WM, Zulian QR, Zinn M, O'Connor K. The chain length of biologically produced (R)-3-hydroxyalkanoic acid affects biological activity and structure of anti-cancer peptides. in Journal of Biotechnology. 2015;204:7-12.
doi:10.1016/j.jbiotec.2015.02.036 .

Szwej, Emilia, Devocelle, Marc, Kenny, Shane, Guzik, Maciej, O'Connor, Stephen, Nikodinović-Runić, Jasmina, Milovanović, Jelena, Maslak, Veselin, Byrne, Annete T., Gallagher, William M., Zulian, Qun Ren, Zinn, Manfred, O'Connor, Kevin , "The chain length of biologically produced (R)-3-hydroxyalkanoic acid affects biological activity and structure of anti-cancer peptides" in Journal of Biotechnology, 204 (2015):7-12,
https://doi.org/10.1016/j.jbiotec.2015.02.036 . .

TY  - JOUR
AU  - Opsenica, Dejan M.
AU  - Milovanović, Jelena
AU  - Matić, Ivana Z.
AU  - Stajner, Tijana
AU  - Knezevic-Usaj, Slavica
AU  - Đurković-Đaković, Olgica
AU  - Solaja, Bogdan A.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/833
AB  - New cyclohexylidene 1,2,4,5-tetraoxanes with polar guanidine and urea based groups were synthesized and evaluated for their antimalarial activity against chloroquine resistant and susceptible Plasmodium falciparum strains. The derivatives showed moderate, nM range antimalarial activities and low cytotoxicity. The N-phenylurea derivative 24 exhibited the best resistance indices (RIW2 = 0.44, RITM91C235 = 0.80) and was not toxic against human normal peripheral blood mononuclear cells (IC50  gt  200 mu M). Seven derivatives were tested in vitro against four human cancer cell lines and they demonstrated high selectivity toward leukaemia K562 cells. One compound, derivative 21 with a primary amino group, was the first tetraoxane tested in vivo against Toxoplasma gondii as another apicomplexan parasite. Subcutaneous administration at a dose of 10 mg kg(-1) day(-1) for 8 days allowed the survival of 20 % of infected mice, thus demonstrating the high potential of tetraoxanes for the treatment of apicomplexan parasites.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Tetraoxanes as inhibitors of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii growth and anti-cancer molecules
EP  - +
IS  - 11
SP  - 1339
VL  - 80
DO  - 10.2298/JSC150430063O
ER  - 

@article{
author = "Opsenica, Dejan M. and Milovanović, Jelena and Matić, Ivana Z. and Stajner, Tijana and Knezevic-Usaj, Slavica and Đurković-Đaković, Olgica and Solaja, Bogdan A.",
year = "2015",
abstract = "New cyclohexylidene 1,2,4,5-tetraoxanes with polar guanidine and urea based groups were synthesized and evaluated for their antimalarial activity against chloroquine resistant and susceptible Plasmodium falciparum strains. The derivatives showed moderate, nM range antimalarial activities and low cytotoxicity. The N-phenylurea derivative 24 exhibited the best resistance indices (RIW2 = 0.44, RITM91C235 = 0.80) and was not toxic against human normal peripheral blood mononuclear cells (IC50  gt  200 mu M). Seven derivatives were tested in vitro against four human cancer cell lines and they demonstrated high selectivity toward leukaemia K562 cells. One compound, derivative 21 with a primary amino group, was the first tetraoxane tested in vivo against Toxoplasma gondii as another apicomplexan parasite. Subcutaneous administration at a dose of 10 mg kg(-1) day(-1) for 8 days allowed the survival of 20 % of infected mice, thus demonstrating the high potential of tetraoxanes for the treatment of apicomplexan parasites.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Tetraoxanes as inhibitors of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii growth and anti-cancer molecules",
pages = "+-1339",
number = "11",
volume = "80",
doi = "10.2298/JSC150430063O"
}

Opsenica, D. M., Milovanović, J., Matić, I. Z., Stajner, T., Knezevic-Usaj, S., Đurković-Đaković, O.,& Solaja, B. A.. (2015). Tetraoxanes as inhibitors of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii growth and anti-cancer molecules. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 80(11), 1339-+.
https://doi.org/10.2298/JSC150430063O

Opsenica DM, Milovanović J, Matić IZ, Stajner T, Knezevic-Usaj S, Đurković-Đaković O, Solaja BA. Tetraoxanes as inhibitors of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii growth and anti-cancer molecules. in Journal of the Serbian Chemical Society. 2015;80(11):1339-+.
doi:10.2298/JSC150430063O .

Opsenica, Dejan M., Milovanović, Jelena, Matić, Ivana Z., Stajner, Tijana, Knezevic-Usaj, Slavica, Đurković-Đaković, Olgica, Solaja, Bogdan A., "Tetraoxanes as inhibitors of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii growth and anti-cancer molecules" in Journal of the Serbian Chemical Society, 80, no. 11 (2015):1339-+,
https://doi.org/10.2298/JSC150430063O . .

TY  - JOUR
AU  - Jovanović, Predrag
AU  - Jeremić, Sanja
AU  - Đokić, Lidija
AU  - Savić, Vladimir
AU  - Milovanović, Jelena
AU  - Maslak, Veselin
AU  - Ivković, Branka
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/716
AB  - Chemoselective reduction of activated carbon-carbon double bond in conjugated nitroalkenes was achieved using Escherichia coli BL21(DE3) whole cells. Nine different substrates have been used furnishing the reduced products in moderate to good yields. 1-Nitro-4-phenyl-1,3-butadiene and (2-nitro-1-propenyl)benzene were successfully biotransformed with corresponding product yields of 54% and 45% respectively. Using this simple and environmentally friendly system 2-(2-nitropropyl)pyridine and 2-(2-nitropropyl)naphthalene were synthesized and characterized for the first time. High substrate conversion efficiency was coupled with low enantioselectivity, however 29% enantiomeric excess was detected in the case of 2-(2-nitropropyl)pyridine. It was shown that electronic properties of the aromatic ring, which affected polarity of the double bond, were not highly influential factors in the reduction process, but the presence of the nitro functionality was essential for the reaction to proceed. 1-Phenyl-4-nitro-1,3-butadiene could not be biotransformed by whole cells of Pseudomonas putida KT2440 or Bacillus subtilis 168 while it was successfully reduced by E. coli DH5 alpha but with lower efficiency in comparison to E. coli BL21(DE3). Knockout mutant affected in nemA gene coding for N-ethylmaleimide reductase (BL21 Delta nemA) could still catalyze bioreductions suggesting multiple active reductases within E. coli BL21(DE3) biocatalyst. The described biocatalytic reduction of substituted nitroalkenes provides an efficient route for the preparation of the corresponding nitroalkanes and introduces the new application of the strain traditionally utilized for recombinant protein expression.
PB  - Elsevier Science Inc, New York
T2  - Enzyme and Microbial Technology
T1  - Chemoselective biocatalytic reduction of conjugated nitroalkenes: New application for an Escherichia coli BL21(DE3) expression strain
EP  - 23
SP  - 16
VL  - 60
DO  - 10.1016/j.enzmictec.2014.03.010
ER  - 

@article{
author = "Jovanović, Predrag and Jeremić, Sanja and Đokić, Lidija and Savić, Vladimir and Milovanović, Jelena and Maslak, Veselin and Ivković, Branka and Vasiljević, Branka and Nikodinović-Runić, Jasmina",
year = "2014",
abstract = "Chemoselective reduction of activated carbon-carbon double bond in conjugated nitroalkenes was achieved using Escherichia coli BL21(DE3) whole cells. Nine different substrates have been used furnishing the reduced products in moderate to good yields. 1-Nitro-4-phenyl-1,3-butadiene and (2-nitro-1-propenyl)benzene were successfully biotransformed with corresponding product yields of 54% and 45% respectively. Using this simple and environmentally friendly system 2-(2-nitropropyl)pyridine and 2-(2-nitropropyl)naphthalene were synthesized and characterized for the first time. High substrate conversion efficiency was coupled with low enantioselectivity, however 29% enantiomeric excess was detected in the case of 2-(2-nitropropyl)pyridine. It was shown that electronic properties of the aromatic ring, which affected polarity of the double bond, were not highly influential factors in the reduction process, but the presence of the nitro functionality was essential for the reaction to proceed. 1-Phenyl-4-nitro-1,3-butadiene could not be biotransformed by whole cells of Pseudomonas putida KT2440 or Bacillus subtilis 168 while it was successfully reduced by E. coli DH5 alpha but with lower efficiency in comparison to E. coli BL21(DE3). Knockout mutant affected in nemA gene coding for N-ethylmaleimide reductase (BL21 Delta nemA) could still catalyze bioreductions suggesting multiple active reductases within E. coli BL21(DE3) biocatalyst. The described biocatalytic reduction of substituted nitroalkenes provides an efficient route for the preparation of the corresponding nitroalkanes and introduces the new application of the strain traditionally utilized for recombinant protein expression.",
publisher = "Elsevier Science Inc, New York",
journal = "Enzyme and Microbial Technology",
title = "Chemoselective biocatalytic reduction of conjugated nitroalkenes: New application for an Escherichia coli BL21(DE3) expression strain",
pages = "23-16",
volume = "60",
doi = "10.1016/j.enzmictec.2014.03.010"
}

Jovanović, P., Jeremić, S., Đokić, L., Savić, V., Milovanović, J., Maslak, V., Ivković, B., Vasiljević, B.,& Nikodinović-Runić, J.. (2014). Chemoselective biocatalytic reduction of conjugated nitroalkenes: New application for an Escherichia coli BL21(DE3) expression strain. in Enzyme and Microbial Technology
Elsevier Science Inc, New York., 60, 16-23.
https://doi.org/10.1016/j.enzmictec.2014.03.010

Jovanović P, Jeremić S, Đokić L, Savić V, Milovanović J, Maslak V, Ivković B, Vasiljević B, Nikodinović-Runić J. Chemoselective biocatalytic reduction of conjugated nitroalkenes: New application for an Escherichia coli BL21(DE3) expression strain. in Enzyme and Microbial Technology. 2014;60:16-23.
doi:10.1016/j.enzmictec.2014.03.010 .

Jovanović, Predrag, Jeremić, Sanja, Đokić, Lidija, Savić, Vladimir, Milovanović, Jelena, Maslak, Veselin, Ivković, Branka, Vasiljević, Branka, Nikodinović-Runić, Jasmina, "Chemoselective biocatalytic reduction of conjugated nitroalkenes: New application for an Escherichia coli BL21(DE3) expression strain" in Enzyme and Microbial Technology, 60 (2014):16-23,
https://doi.org/10.1016/j.enzmictec.2014.03.010 . .

TY  - JOUR
AU  - Milovanović, Jelena
AU  - Minovska, Gordana
AU  - Šenerović, Lidija
AU  - O'Connor, Kevin
AU  - Jovanović, Predrag
AU  - Savić, Vladimir
AU  - Tokić-Vujosević, Zorana
AU  - Nikodinović-Runić, Jasmina
AU  - Maslak, Veselin
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/759
AB  - Synthesis of gamma-nitroaldehydes from branched chain aldehydes and a range of alpha,beta-unsaturated nitroalkenes was achieved by a whole-cell biocatalytic reaction using 4-oxalocrotonate tautomerase as catalyst. Under mild conditions, cyclic and acyclic branched aldehydes were converted into synthetically valuable quaternary carbon containing gamma-nitroaldehydes. The yields of the desired products were influenced by reaction condition parameters such as organic solvent, temperature and pH. The whole-cell biocatalytic approach to the generation of alpha,alpha-substituted gamma-nitroaldehydes was compared to the organocatalytic approach involving the lithium salt of phenylalanine as a catalyst. As the resulting gamma-nitroaldehydes exhibited moderate antifungal activity and mild in vitro cytotoxicity against human fibroblasts (0.2-0.4 mM) they could further be examined as potentially useful pharmaceutical synthons.
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Synthesis of gamma-nitroaldehydes containing quaternary carbon in the alpha-position using a 4-oxalocrotonate tautomerase whole-cell biocatalyst
EP  - 60510
IS  - 105
SP  - 60502
VL  - 4
DO  - 10.1039/c4ra05517a
ER  - 

@article{
author = "Milovanović, Jelena and Minovska, Gordana and Šenerović, Lidija and O'Connor, Kevin and Jovanović, Predrag and Savić, Vladimir and Tokić-Vujosević, Zorana and Nikodinović-Runić, Jasmina and Maslak, Veselin",
year = "2014",
abstract = "Synthesis of gamma-nitroaldehydes from branched chain aldehydes and a range of alpha,beta-unsaturated nitroalkenes was achieved by a whole-cell biocatalytic reaction using 4-oxalocrotonate tautomerase as catalyst. Under mild conditions, cyclic and acyclic branched aldehydes were converted into synthetically valuable quaternary carbon containing gamma-nitroaldehydes. The yields of the desired products were influenced by reaction condition parameters such as organic solvent, temperature and pH. The whole-cell biocatalytic approach to the generation of alpha,alpha-substituted gamma-nitroaldehydes was compared to the organocatalytic approach involving the lithium salt of phenylalanine as a catalyst. As the resulting gamma-nitroaldehydes exhibited moderate antifungal activity and mild in vitro cytotoxicity against human fibroblasts (0.2-0.4 mM) they could further be examined as potentially useful pharmaceutical synthons.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Synthesis of gamma-nitroaldehydes containing quaternary carbon in the alpha-position using a 4-oxalocrotonate tautomerase whole-cell biocatalyst",
pages = "60510-60502",
number = "105",
volume = "4",
doi = "10.1039/c4ra05517a"
}

Milovanović, J., Minovska, G., Šenerović, L., O'Connor, K., Jovanović, P., Savić, V., Tokić-Vujosević, Z., Nikodinović-Runić, J.,& Maslak, V.. (2014). Synthesis of gamma-nitroaldehydes containing quaternary carbon in the alpha-position using a 4-oxalocrotonate tautomerase whole-cell biocatalyst. in RSC Advances
Royal Soc Chemistry, Cambridge., 4(105), 60502-60510.
https://doi.org/10.1039/c4ra05517a

Milovanović J, Minovska G, Šenerović L, O'Connor K, Jovanović P, Savić V, Tokić-Vujosević Z, Nikodinović-Runić J, Maslak V. Synthesis of gamma-nitroaldehydes containing quaternary carbon in the alpha-position using a 4-oxalocrotonate tautomerase whole-cell biocatalyst. in RSC Advances. 2014;4(105):60502-60510.
doi:10.1039/c4ra05517a .

Milovanović, Jelena, Minovska, Gordana, Šenerović, Lidija, O'Connor, Kevin, Jovanović, Predrag, Savić, Vladimir, Tokić-Vujosević, Zorana, Nikodinović-Runić, Jasmina, Maslak, Veselin, "Synthesis of gamma-nitroaldehydes containing quaternary carbon in the alpha-position using a 4-oxalocrotonate tautomerase whole-cell biocatalyst" in RSC Advances, 4, no. 105 (2014):60502-60510,
https://doi.org/10.1039/c4ra05517a . .

TY  - JOUR
AU  - Narancić, Tanja
AU  - Milovanović, Jelena
AU  - Jovanović, Predrag
AU  - Francuski, Djordje
AU  - Bigović, Miljan
AU  - Maslak, Veselin
AU  - Savić, Vladimir
AU  - Vasiljević, Branka
AU  - O'Connor, Kevin 
AU  - Nikodinović-Runić, Jasmina
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/656
AB  - A novel whole cell system based on recombinantly expressed 4-oxalocrotonate tautomerase (4-OT) was developed and shown to be an effective biocatalyst for the asymmetric Michael addition of acetaldehyde to beta-nitrostyrenes. Optimal ratio of substrates (2 mM beta-nitrostyrenes and 20 mM acetaldehyde) and biocatalyst of 5 g of cell dry weight of biocatalyst per liter was determined. Through further bioprocess improvement by sequential addition of substrate 10 mM nitrostyrene biotransformation was achieved within 150 min. Excellent enantioselectivity ( gt 99% ee) and product yields of up to 60% were obtained with beta-nitrostyrene substrate. The biotransformation product, 4-nitro-3-phenyl-butanal, was isolated from aqueous media and further transformed into the corresponding amino alcohol. The biocatalyst exhibited lower reaction rates with p-Cl-, o-Cl- and p-F-beta-nitrostyrenes with product yields of 38%, 51%, 31% and ee values of 84%, 88% and 94% respectively. The importance of the terminal,proline of 4-UT was confirmed by two proline enriched variants and homology modeling.
PB  - Elsevier Sci Ltd, Oxford
T2  - Bioresource Technology
T1  - Highly efficient Michael-type addition of acetaldehyde to beta-nitrostyrenes by whole resting cells of Escherichia coli expressing 4-oxalocrotonate tautomerase
EP  - 468
SP  - 462
VL  - 142
DO  - 10.1016/j.biortech.2013.05.074
ER  - 

@article{
author = "Narancić, Tanja and Milovanović, Jelena and Jovanović, Predrag and Francuski, Djordje and Bigović, Miljan and Maslak, Veselin and Savić, Vladimir and Vasiljević, Branka and O'Connor, Kevin  and Nikodinović-Runić, Jasmina",
year = "2013",
abstract = "A novel whole cell system based on recombinantly expressed 4-oxalocrotonate tautomerase (4-OT) was developed and shown to be an effective biocatalyst for the asymmetric Michael addition of acetaldehyde to beta-nitrostyrenes. Optimal ratio of substrates (2 mM beta-nitrostyrenes and 20 mM acetaldehyde) and biocatalyst of 5 g of cell dry weight of biocatalyst per liter was determined. Through further bioprocess improvement by sequential addition of substrate 10 mM nitrostyrene biotransformation was achieved within 150 min. Excellent enantioselectivity ( gt 99% ee) and product yields of up to 60% were obtained with beta-nitrostyrene substrate. The biotransformation product, 4-nitro-3-phenyl-butanal, was isolated from aqueous media and further transformed into the corresponding amino alcohol. The biocatalyst exhibited lower reaction rates with p-Cl-, o-Cl- and p-F-beta-nitrostyrenes with product yields of 38%, 51%, 31% and ee values of 84%, 88% and 94% respectively. The importance of the terminal,proline of 4-UT was confirmed by two proline enriched variants and homology modeling.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Bioresource Technology",
title = "Highly efficient Michael-type addition of acetaldehyde to beta-nitrostyrenes by whole resting cells of Escherichia coli expressing 4-oxalocrotonate tautomerase",
pages = "468-462",
volume = "142",
doi = "10.1016/j.biortech.2013.05.074"
}

Narancić, T., Milovanović, J., Jovanović, P., Francuski, D., Bigović, M., Maslak, V., Savić, V., Vasiljević, B., O'Connor, K.,& Nikodinović-Runić, J.. (2013). Highly efficient Michael-type addition of acetaldehyde to beta-nitrostyrenes by whole resting cells of Escherichia coli expressing 4-oxalocrotonate tautomerase. in Bioresource Technology
Elsevier Sci Ltd, Oxford., 142, 462-468.
https://doi.org/10.1016/j.biortech.2013.05.074

Narancić T, Milovanović J, Jovanović P, Francuski D, Bigović M, Maslak V, Savić V, Vasiljević B, O'Connor K, Nikodinović-Runić J. Highly efficient Michael-type addition of acetaldehyde to beta-nitrostyrenes by whole resting cells of Escherichia coli expressing 4-oxalocrotonate tautomerase. in Bioresource Technology. 2013;142:462-468.
doi:10.1016/j.biortech.2013.05.074 .

Narancić, Tanja, Milovanović, Jelena, Jovanović, Predrag, Francuski, Djordje, Bigović, Miljan, Maslak, Veselin, Savić, Vladimir, Vasiljević, Branka, O'Connor, Kevin , Nikodinović-Runić, Jasmina, "Highly efficient Michael-type addition of acetaldehyde to beta-nitrostyrenes by whole resting cells of Escherichia coli expressing 4-oxalocrotonate tautomerase" in Bioresource Technology, 142 (2013):462-468,
https://doi.org/10.1016/j.biortech.2013.05.074 . .