TY  - JOUR
AU  - Soković Bajić, Svetlana
AU  - Mihajlović, Sanja B.
AU  - Radojević, Dušan
AU  - Popović, Dušanka
AU  - Đokić, Jelena
AU  - Stanisavljević, Suzana
AU  - Lazarević, Milica N.
AU  - Miljković, Djordje M.
AU  - Ruas-Madiedo, Patricia
AU  - Golić, Nataša
AU  - Tolinački, Maja
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1386
AB  - Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system. The aim of this work was to study the probiotic effect of gamma-aminobutyric acid (GABA)-producer Lactobacillus brevis BGZLS10-17 on experimental autoimmune encephalomyelitis (EAE), an experimental animal MS model. Clinical EAE symptoms were monitored in Dark Agouti (DA) rats treated with L. brevis BGZLS10-17 strain, or supernatant obtained from 48 h culture of L. brevis BGZLS10-17 cultivated in growth medium with or without GABA precursor monosodium glutamate (MSG). The results revealed that oral administration of L. brevis BGZLS10-17 alleviates the symptoms of EAE in DA rats. Namely, treatment with BGZLS10-17 and the supernatant of the strain cultivated in medium with MSG delayed the onset, shortened the duration, and reduced the intensity of the disease in the period when the EAE symptoms in controls were most pronounced. The probiotic treated animals were completely recovered after forty days, unlike the control animals. The results indicate that supplementation with live strain or with supernatant containing GABA produced by L. brevis BGZLS10-17 could alleviate the EAE symptoms. However, the use of L. brevis BGZLS10-17 in functional food as probiotic for autoimmune diseases should be tested in clinical trials.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Characterization of pH resistance and the proteolytic activity of GABA producing Lactobacillus brevis BGZLS10-17 in preparation of fermented milk beverage and the effects on the symptoms of the experimental autoimmune encephalomyelitis
EP  - 176
IS  - 2
SP  - 163
VL  - 85
DO  - 10.2298/JSC190716094S
ER  - 

@article{
author = "Soković Bajić, Svetlana and Mihajlović, Sanja B. and Radojević, Dušan and Popović, Dušanka and Đokić, Jelena and Stanisavljević, Suzana and Lazarević, Milica N. and Miljković, Djordje M. and Ruas-Madiedo, Patricia and Golić, Nataša and Tolinački, Maja",
year = "2020",
abstract = "Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system. The aim of this work was to study the probiotic effect of gamma-aminobutyric acid (GABA)-producer Lactobacillus brevis BGZLS10-17 on experimental autoimmune encephalomyelitis (EAE), an experimental animal MS model. Clinical EAE symptoms were monitored in Dark Agouti (DA) rats treated with L. brevis BGZLS10-17 strain, or supernatant obtained from 48 h culture of L. brevis BGZLS10-17 cultivated in growth medium with or without GABA precursor monosodium glutamate (MSG). The results revealed that oral administration of L. brevis BGZLS10-17 alleviates the symptoms of EAE in DA rats. Namely, treatment with BGZLS10-17 and the supernatant of the strain cultivated in medium with MSG delayed the onset, shortened the duration, and reduced the intensity of the disease in the period when the EAE symptoms in controls were most pronounced. The probiotic treated animals were completely recovered after forty days, unlike the control animals. The results indicate that supplementation with live strain or with supernatant containing GABA produced by L. brevis BGZLS10-17 could alleviate the EAE symptoms. However, the use of L. brevis BGZLS10-17 in functional food as probiotic for autoimmune diseases should be tested in clinical trials.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Characterization of pH resistance and the proteolytic activity of GABA producing Lactobacillus brevis BGZLS10-17 in preparation of fermented milk beverage and the effects on the symptoms of the experimental autoimmune encephalomyelitis",
pages = "176-163",
number = "2",
volume = "85",
doi = "10.2298/JSC190716094S"
}

Soković Bajić, S., Mihajlović, S. B., Radojević, D., Popović, D., Đokić, J., Stanisavljević, S., Lazarević, M. N., Miljković, D. M., Ruas-Madiedo, P., Golić, N.,& Tolinački, M.. (2020). Characterization of pH resistance and the proteolytic activity of GABA producing Lactobacillus brevis BGZLS10-17 in preparation of fermented milk beverage and the effects on the symptoms of the experimental autoimmune encephalomyelitis. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 85(2), 163-176.
https://doi.org/10.2298/JSC190716094S

Soković Bajić S, Mihajlović SB, Radojević D, Popović D, Đokić J, Stanisavljević S, Lazarević MN, Miljković DM, Ruas-Madiedo P, Golić N, Tolinački M. Characterization of pH resistance and the proteolytic activity of GABA producing Lactobacillus brevis BGZLS10-17 in preparation of fermented milk beverage and the effects on the symptoms of the experimental autoimmune encephalomyelitis. in Journal of the Serbian Chemical Society. 2020;85(2):163-176.
doi:10.2298/JSC190716094S .

Soković Bajić, Svetlana, Mihajlović, Sanja B., Radojević, Dušan, Popović, Dušanka, Đokić, Jelena, Stanisavljević, Suzana, Lazarević, Milica N., Miljković, Djordje M., Ruas-Madiedo, Patricia, Golić, Nataša, Tolinački, Maja, "Characterization of pH resistance and the proteolytic activity of GABA producing Lactobacillus brevis BGZLS10-17 in preparation of fermented milk beverage and the effects on the symptoms of the experimental autoimmune encephalomyelitis" in Journal of the Serbian Chemical Society, 85, no. 2 (2020):163-176,
https://doi.org/10.2298/JSC190716094S . .

TY  - JOUR
AU  - Mijatović, Sanja A.
AU  - Timotijević, Gordana
AU  - Miljković, Djordje M.
AU  - Radović, Julijana M.
AU  - Maksimović-Ivanić, Danijela
AU  - Dekanski, Dragana P.
AU  - Stošić-Grujičić, Stanislava
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/486
AB  - Various constituents of the olive tree (Olea europaea) have been traditionally used in the treatment of infection, inflammation, prevention of chronic diseases, cardiovascular disorders and cancer. The anticancer potential of dry olive leaf extract (DOLE) represents the net effect of multilevel interactions between different biologically active compounds from the extract, cancer cells and conventional therapy. In this context, it was of primary interest to evaluate the influence of DOLE on progression of the highly malignant, immuno-and chemoresistant type of skin cancer-melanoma. DOLE significantly inhibited proliferation and subsequently restricted clonogenicity of the B16 mouse melanoma cell line in vitro. Moreover, late phase tumor treatment with DOLE significantly reduced tumor volume in a syngeneic strain of mice. DOLE-treated B16 cells were blocked in the G(0)/G(1) phase of the cell cycle, underwent early apoptosis and died by late necrosis. At the molecular level, the dying process started as caspase dependent, but finalized as caspase independent. In concordance, overexpression of antiapoptotic members of the Bcl-2 family, Bcl-2 and Bcl-XL, and diminished expression of their natural antagonists, Bim and p53, were observed. Despite molecular suppression of the proapoptotic process, DOLE successfully promoted cell death mainly through disruption of cell membrane integrity and late caspase-independent fragmentation of genetic material. Taken together, the results of this study indicate that DOLE possesses strong antimelanoma potential. When DOLE was applied in combination with different chemotherapeutics, various outcomes, including synergy and antagonism, were observed. This requires caution in the use of the extract as a supplementary antitumor therapeutic.
PB  - Wiley, Hoboken
T2  - International Journal of Cancer
T1  - Multiple antimelanoma potential of dry olive leaf extract
EP  - 1965
IS  - 8
SP  - 1955
VL  - 128
DO  - 10.1002/ijc.25526
ER  - 

@article{
author = "Mijatović, Sanja A. and Timotijević, Gordana and Miljković, Djordje M. and Radović, Julijana M. and Maksimović-Ivanić, Danijela and Dekanski, Dragana P. and Stošić-Grujičić, Stanislava",
year = "2011",
abstract = "Various constituents of the olive tree (Olea europaea) have been traditionally used in the treatment of infection, inflammation, prevention of chronic diseases, cardiovascular disorders and cancer. The anticancer potential of dry olive leaf extract (DOLE) represents the net effect of multilevel interactions between different biologically active compounds from the extract, cancer cells and conventional therapy. In this context, it was of primary interest to evaluate the influence of DOLE on progression of the highly malignant, immuno-and chemoresistant type of skin cancer-melanoma. DOLE significantly inhibited proliferation and subsequently restricted clonogenicity of the B16 mouse melanoma cell line in vitro. Moreover, late phase tumor treatment with DOLE significantly reduced tumor volume in a syngeneic strain of mice. DOLE-treated B16 cells were blocked in the G(0)/G(1) phase of the cell cycle, underwent early apoptosis and died by late necrosis. At the molecular level, the dying process started as caspase dependent, but finalized as caspase independent. In concordance, overexpression of antiapoptotic members of the Bcl-2 family, Bcl-2 and Bcl-XL, and diminished expression of their natural antagonists, Bim and p53, were observed. Despite molecular suppression of the proapoptotic process, DOLE successfully promoted cell death mainly through disruption of cell membrane integrity and late caspase-independent fragmentation of genetic material. Taken together, the results of this study indicate that DOLE possesses strong antimelanoma potential. When DOLE was applied in combination with different chemotherapeutics, various outcomes, including synergy and antagonism, were observed. This requires caution in the use of the extract as a supplementary antitumor therapeutic.",
publisher = "Wiley, Hoboken",
journal = "International Journal of Cancer",
title = "Multiple antimelanoma potential of dry olive leaf extract",
pages = "1965-1955",
number = "8",
volume = "128",
doi = "10.1002/ijc.25526"
}

Mijatović, S. A., Timotijević, G., Miljković, D. M., Radović, J. M., Maksimović-Ivanić, D., Dekanski, D. P.,& Stošić-Grujičić, S.. (2011). Multiple antimelanoma potential of dry olive leaf extract. in International Journal of Cancer
Wiley, Hoboken., 128(8), 1955-1965.
https://doi.org/10.1002/ijc.25526

Mijatović SA, Timotijević G, Miljković DM, Radović JM, Maksimović-Ivanić D, Dekanski DP, Stošić-Grujičić S. Multiple antimelanoma potential of dry olive leaf extract. in International Journal of Cancer. 2011;128(8):1955-1965.
doi:10.1002/ijc.25526 .

Mijatović, Sanja A., Timotijević, Gordana, Miljković, Djordje M., Radović, Julijana M., Maksimović-Ivanić, Danijela, Dekanski, Dragana P., Stošić-Grujičić, Stanislava, "Multiple antimelanoma potential of dry olive leaf extract" in International Journal of Cancer, 128, no. 8 (2011):1955-1965,
https://doi.org/10.1002/ijc.25526 . .