TY  - CONF
AU  - Mesaroš, Aleksandra
AU  - Atanasković, Iva
AU  - Jakovljević, Stefan
AU  - Stević, Tatjana
AU  - Dinić, Miroslav
AU  - Lozo, Jelena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2152
AB  - Introduction: Hydrogen cyanide (HCN) is a volatile secondary metabolite synthesized by some bacteria, and this ability enablestheir activity against various pathogens. The aim of thisstudy wasto identify
HCN-producing bacteria and investigate their biocontrol potential.
Methods: Three HCN-producing strains were detected in a collection of bell pepper plant isolates using
a semi-quantitative assay with picric acid. The presence of hcnABC operon genes was confirmed by PCR.
The biological control potential of the HCN-producing strains wastested against three fungal (Fusarium
oxysporum, Rhizoctonia solani, Verticillium dahliae) and eight bacterial (genera Xanthomonas,
Pseudomonas and Clavibacter) pathogens of bell pepper plants in a split-section Petri dish experiment.
The potential nematocidal activity was demonstrated by using the Caenorhabditis elegans AU37 strain,
with temperature-sensitive sterility and enhanced sensitivity to pathogens.
Results: Detailed characterization of 300 isolates from our collection revealed that we have three different HCN-producing strains identified as Bacillussubtilis, Pseudomonas moraviensis, and P. putida, with
P. putida A32 being the most potent. Thisstrain is used for the deletion of the hcnB gene to confirm HCN
as a biocontrol agent.
Conclusion: The HCN-producing strains showed biocontrol potential against bacteria, fungi, and nematodes. It is concluded that the biological control activity isthe result of a volatile metabolite diffusing
through the air. Our future exp
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Determination of hydrogen cyanide producing strains as potential biocontrol agents
EP  - 116
SP  - 116
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2152
ER  - 

@conference{
author = "Mesaroš, Aleksandra and Atanasković, Iva and Jakovljević, Stefan and Stević, Tatjana and Dinić, Miroslav and Lozo, Jelena",
year = "2023",
abstract = "Introduction: Hydrogen cyanide (HCN) is a volatile secondary metabolite synthesized by some bacteria, and this ability enablestheir activity against various pathogens. The aim of thisstudy wasto identify
HCN-producing bacteria and investigate their biocontrol potential.
Methods: Three HCN-producing strains were detected in a collection of bell pepper plant isolates using
a semi-quantitative assay with picric acid. The presence of hcnABC operon genes was confirmed by PCR.
The biological control potential of the HCN-producing strains wastested against three fungal (Fusarium
oxysporum, Rhizoctonia solani, Verticillium dahliae) and eight bacterial (genera Xanthomonas,
Pseudomonas and Clavibacter) pathogens of bell pepper plants in a split-section Petri dish experiment.
The potential nematocidal activity was demonstrated by using the Caenorhabditis elegans AU37 strain,
with temperature-sensitive sterility and enhanced sensitivity to pathogens.
Results: Detailed characterization of 300 isolates from our collection revealed that we have three different HCN-producing strains identified as Bacillussubtilis, Pseudomonas moraviensis, and P. putida, with
P. putida A32 being the most potent. Thisstrain is used for the deletion of the hcnB gene to confirm HCN
as a biocontrol agent.
Conclusion: The HCN-producing strains showed biocontrol potential against bacteria, fungi, and nematodes. It is concluded that the biological control activity isthe result of a volatile metabolite diffusing
through the air. Our future exp",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Determination of hydrogen cyanide producing strains as potential biocontrol agents",
pages = "116-116",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2152"
}

Mesaroš, A., Atanasković, I., Jakovljević, S., Stević, T., Dinić, M.,& Lozo, J.. (2023). Determination of hydrogen cyanide producing strains as potential biocontrol agents. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 116-116.
https://hdl.handle.net/21.15107/rcub_imagine_2152

Mesaroš A, Atanasković I, Jakovljević S, Stević T, Dinić M, Lozo J. Determination of hydrogen cyanide producing strains as potential biocontrol agents. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:116-116.
https://hdl.handle.net/21.15107/rcub_imagine_2152 .

Mesaroš, Aleksandra, Atanasković, Iva, Jakovljević, Stefan, Stević, Tatjana, Dinić, Miroslav, Lozo, Jelena, "Determination of hydrogen cyanide producing strains as potential biocontrol agents" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):116-116,
https://hdl.handle.net/21.15107/rcub_imagine_2152 .

TY  - JOUR
AU  - Lozo, Jelena
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1503
AB  - Microorganisms isolated from various traditionally fermented food products prepared in households without commercial starter cultures are designated as natural isolates. In addition, this term is also used for microorganisms collected from various natural habitats or products (silage, soil, manure, plant and animal material, etc.) that do not contain any commercial starters or bacterial formulations. They are characterized by unique traits that are the result of the selective pressure of environmental conditions, as well as interactions with other organisms. The synthesis of antimicrobial molecules, including bacteriocins, is an evolutionary advantage and an adaptive feature that sets them apart from other microorganisms from a common environment. This review aims to underline the knowledge of bacteriocins produced by natural isolates, with a particular emphasis on the most common location of their genes and operons, plasmids, and the importance of the relationship between the plasmidome and the adaptive potential of the isolate. Applications of bacteriocins, ranging from natural food preservatives to supplements and drugs in pharmacology and medicine, will also be addressed. The latest challenges faced by researchers in isolating new natural isolates with desired characteristics will be discussed, as well as the production of new antimicrobials, nearly one century since the first discovery of colicins in 1925.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Natural bacterial isolates as an inexhaustible source of new bacteriocins
EP  - 492
IS  - 2
SP  - 477
VL  - 105
DO  - 10.1007/s00253-020-11063-3
ER  - 

@article{
author = "Lozo, Jelena and Topisirović, Ljubiša and Kojić, Milan",
year = "2021",
abstract = "Microorganisms isolated from various traditionally fermented food products prepared in households without commercial starter cultures are designated as natural isolates. In addition, this term is also used for microorganisms collected from various natural habitats or products (silage, soil, manure, plant and animal material, etc.) that do not contain any commercial starters or bacterial formulations. They are characterized by unique traits that are the result of the selective pressure of environmental conditions, as well as interactions with other organisms. The synthesis of antimicrobial molecules, including bacteriocins, is an evolutionary advantage and an adaptive feature that sets them apart from other microorganisms from a common environment. This review aims to underline the knowledge of bacteriocins produced by natural isolates, with a particular emphasis on the most common location of their genes and operons, plasmids, and the importance of the relationship between the plasmidome and the adaptive potential of the isolate. Applications of bacteriocins, ranging from natural food preservatives to supplements and drugs in pharmacology and medicine, will also be addressed. The latest challenges faced by researchers in isolating new natural isolates with desired characteristics will be discussed, as well as the production of new antimicrobials, nearly one century since the first discovery of colicins in 1925.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Natural bacterial isolates as an inexhaustible source of new bacteriocins",
pages = "492-477",
number = "2",
volume = "105",
doi = "10.1007/s00253-020-11063-3"
}

Lozo, J., Topisirović, L.,& Kojić, M.. (2021). Natural bacterial isolates as an inexhaustible source of new bacteriocins. in Applied Microbiology and Biotechnology
Springer, New York., 105(2), 477-492.
https://doi.org/10.1007/s00253-020-11063-3

Lozo J, Topisirović L, Kojić M. Natural bacterial isolates as an inexhaustible source of new bacteriocins. in Applied Microbiology and Biotechnology. 2021;105(2):477-492.
doi:10.1007/s00253-020-11063-3 .

Lozo, Jelena, Topisirović, Ljubiša, Kojić, Milan, "Natural bacterial isolates as an inexhaustible source of new bacteriocins" in Applied Microbiology and Biotechnology, 105, no. 2 (2021):477-492,
https://doi.org/10.1007/s00253-020-11063-3 . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Lukić, Jovanka
AU  - Lozo, Jelena
AU  - Fira, Đorđe
AU  - Jovčić, Branko
AU  - Strahinić, Ivana
AU  - Begović, Jelena
AU  - Popović, Nikola
AU  - Miljković, Marija
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
AU  - Golić, Nataša
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1353
AB  - The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.
PB  - Elsevier, Amsterdam
T2  - Food Research International
T1  - Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties
VL  - 136
DO  - 10.1016/j.foodres.2020.109494
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Tolinački, Maja and Živković, Milica and Lukić, Jovanka and Lozo, Jelena and Fira, Đorđe and Jovčić, Branko and Strahinić, Ivana and Begović, Jelena and Popović, Nikola and Miljković, Marija and Kojić, Milan and Topisirović, Ljubiša and Golić, Nataša",
year = "2020",
abstract = "The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.",
publisher = "Elsevier, Amsterdam",
journal = "Food Research International",
title = "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties",
volume = "136",
doi = "10.1016/j.foodres.2020.109494"
}

Terzić-Vidojević, A., Veljović, K., Tolinački, M., Živković, M., Lukić, J., Lozo, J., Fira, Đ., Jovčić, B., Strahinić, I., Begović, J., Popović, N., Miljković, M., Kojić, M., Topisirović, L.,& Golić, N.. (2020). Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International
Elsevier, Amsterdam., 136.
https://doi.org/10.1016/j.foodres.2020.109494

Terzić-Vidojević A, Veljović K, Tolinački M, Živković M, Lukić J, Lozo J, Fira Đ, Jovčić B, Strahinić I, Begović J, Popović N, Miljković M, Kojić M, Topisirović L, Golić N. Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International. 2020;136.
doi:10.1016/j.foodres.2020.109494 .

Terzić-Vidojević, Amarela, Veljović, Katarina, Tolinački, Maja, Živković, Milica, Lukić, Jovanka, Lozo, Jelena, Fira, Đorđe, Jovčić, Branko, Strahinić, Ivana, Begović, Jelena, Popović, Nikola, Miljković, Marija, Kojić, Milan, Topisirović, Ljubiša, Golić, Nataša, "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties" in Food Research International, 136 (2020),
https://doi.org/10.1016/j.foodres.2020.109494 . .

TY  - JOUR
AU  - Klisurić, Olivera R.
AU  - Armaković, Sanja J.
AU  - Armaković, Stevan
AU  - Marković, Sanja
AU  - Todorović, Tamara R.
AU  - Portalone, Gustavo
AU  - Novović, Katarina
AU  - Lozo, Jelena
AU  - Filipović, Nenad R.
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1405
AB  - In this work pharmaceutical application of focused library of six quinoline-based chalcogensemicarbazones (QBCs) was tested through determination of their antimicrobial activity against twenty-eight Gram-negative and Gram-positive strains from different origin. Pharmacokinetic properties have been assessed by the analysis of frequently employed drug likeness parameters. Computational study has been complemented with calculation of their global and local reactive properties, within the framework of density functional theory (DFT). Among other information, DFT calculations helped us to locate the most reactive sites of investigated QBCs and to identify their sensitivity towards the oxidation.
PB  - Elsevier, Amsterdam
T2  - Journal of Molecular Structure
T1  - Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones
VL  - 1203
DO  - 10.1016/j.molstruc.2019.127482
ER  - 

@article{
author = "Klisurić, Olivera R. and Armaković, Sanja J. and Armaković, Stevan and Marković, Sanja and Todorović, Tamara R. and Portalone, Gustavo and Novović, Katarina and Lozo, Jelena and Filipović, Nenad R.",
year = "2020",
abstract = "In this work pharmaceutical application of focused library of six quinoline-based chalcogensemicarbazones (QBCs) was tested through determination of their antimicrobial activity against twenty-eight Gram-negative and Gram-positive strains from different origin. Pharmacokinetic properties have been assessed by the analysis of frequently employed drug likeness parameters. Computational study has been complemented with calculation of their global and local reactive properties, within the framework of density functional theory (DFT). Among other information, DFT calculations helped us to locate the most reactive sites of investigated QBCs and to identify their sensitivity towards the oxidation.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Molecular Structure",
title = "Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones",
volume = "1203",
doi = "10.1016/j.molstruc.2019.127482"
}

Klisurić, O. R., Armaković, S. J., Armaković, S., Marković, S., Todorović, T. R., Portalone, G., Novović, K., Lozo, J.,& Filipović, N. R.. (2020). Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones. in Journal of Molecular Structure
Elsevier, Amsterdam., 1203.
https://doi.org/10.1016/j.molstruc.2019.127482

Klisurić OR, Armaković SJ, Armaković S, Marković S, Todorović TR, Portalone G, Novović K, Lozo J, Filipović NR. Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones. in Journal of Molecular Structure. 2020;1203.
doi:10.1016/j.molstruc.2019.127482 .

Klisurić, Olivera R., Armaković, Sanja J., Armaković, Stevan, Marković, Sanja, Todorović, Tamara R., Portalone, Gustavo, Novović, Katarina, Lozo, Jelena, Filipović, Nenad R., "Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones" in Journal of Molecular Structure, 1203 (2020),
https://doi.org/10.1016/j.molstruc.2019.127482 . .

TY  - JOUR
AU  - Malešević, Milka
AU  - Mirković, Nemanja
AU  - Lozo, Jelena
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1297
AB  - 16S rRNA gene-based metagenomic approach was used to assess the biodiversity of bacterial communities in the sediments of selected glacial lakes in the Western Balkans and to assess the impact of human population on these microbial communities. Sediment samples were collected from three glacial lakes, viz., Plav Lake (in a zone of the highest impact of human population), Black Lake (a zone of medium impact of human population), and Donje Bare Lake (a remote lake with minimal impact of human population). Canonical correlation analysis analysis indicated correlation between the distance of the lake from urbanized population and bacterial diversity in Donje Bare Lake sediment. Bacterial diversity of Black Lake sediment was correlated with high content of phosphorous and pH value. Chemical compounds exhibiting the most prominent correlation with bacterial diversity of Plav Lake were NH4-N, K2O, CaCo3, and total nitrogen . Additionally, CCA analysis indicated that population density was correlated with biodiversity of bacterial communities in Plav Lake sediment, which is the most exposed to human population. Multivariate regression revealed the highest correlation between the presence of Proteobacteria classes and population density and levels of NH4-N. The influence of human population was observed to be important for shaping the sediment communities in addition to biological and chemical factors.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Geomicrobiology Journal
T1  - Bacterial Diversity among the Sediments of Glacial Lakes in the Western Balkans: Exploring the Impact of Human Population
EP  - 270
IS  - 3
SP  - 261
VL  - 36
DO  - 10.1080/01490451.2018.1550128
ER  - 

@article{
author = "Malešević, Milka and Mirković, Nemanja and Lozo, Jelena and Novović, Katarina and Filipić, Brankica and Kojić, Milan and Jovčić, Branko",
year = "2019",
abstract = "16S rRNA gene-based metagenomic approach was used to assess the biodiversity of bacterial communities in the sediments of selected glacial lakes in the Western Balkans and to assess the impact of human population on these microbial communities. Sediment samples were collected from three glacial lakes, viz., Plav Lake (in a zone of the highest impact of human population), Black Lake (a zone of medium impact of human population), and Donje Bare Lake (a remote lake with minimal impact of human population). Canonical correlation analysis analysis indicated correlation between the distance of the lake from urbanized population and bacterial diversity in Donje Bare Lake sediment. Bacterial diversity of Black Lake sediment was correlated with high content of phosphorous and pH value. Chemical compounds exhibiting the most prominent correlation with bacterial diversity of Plav Lake were NH4-N, K2O, CaCo3, and total nitrogen . Additionally, CCA analysis indicated that population density was correlated with biodiversity of bacterial communities in Plav Lake sediment, which is the most exposed to human population. Multivariate regression revealed the highest correlation between the presence of Proteobacteria classes and population density and levels of NH4-N. The influence of human population was observed to be important for shaping the sediment communities in addition to biological and chemical factors.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Geomicrobiology Journal",
title = "Bacterial Diversity among the Sediments of Glacial Lakes in the Western Balkans: Exploring the Impact of Human Population",
pages = "270-261",
number = "3",
volume = "36",
doi = "10.1080/01490451.2018.1550128"
}

Malešević, M., Mirković, N., Lozo, J., Novović, K., Filipić, B., Kojić, M.,& Jovčić, B.. (2019). Bacterial Diversity among the Sediments of Glacial Lakes in the Western Balkans: Exploring the Impact of Human Population. in Geomicrobiology Journal
Taylor & Francis Inc, Philadelphia., 36(3), 261-270.
https://doi.org/10.1080/01490451.2018.1550128

Malešević M, Mirković N, Lozo J, Novović K, Filipić B, Kojić M, Jovčić B. Bacterial Diversity among the Sediments of Glacial Lakes in the Western Balkans: Exploring the Impact of Human Population. in Geomicrobiology Journal. 2019;36(3):261-270.
doi:10.1080/01490451.2018.1550128 .

Malešević, Milka, Mirković, Nemanja, Lozo, Jelena, Novović, Katarina, Filipić, Brankica, Kojić, Milan, Jovčić, Branko, "Bacterial Diversity among the Sediments of Glacial Lakes in the Western Balkans: Exploring the Impact of Human Population" in Geomicrobiology Journal, 36, no. 3 (2019):261-270,
https://doi.org/10.1080/01490451.2018.1550128 . .

TY  - JOUR
AU  - Miljković, Manja
AU  - Lozo, Jelena
AU  - Mirković, Nemanja
AU  - O'Connor, Paula M.
AU  - Malešević, Milka
AU  - Jovčić, Branko
AU  - Cotter, Paul D.
AU  - Kojić, Milan
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1124
AB  - The gene cluster responsible for the production of the aureocin A53-like bacteriocin, lactolisterin BU, is located on plasmid pBU6 in Lactococcus lactis subsp. lactis BGBU1-4. Heterologous expression of pBU6 confirmed that production and limited immunity to lactolisterin BU were provided by the plasmid. Comparative analysis of aureocin A53-like operons revealed that the structural genes shared a low level of identity, while other genes were without homology, indicating a different origin. Subcloning and expression of genes located downstream of the structural gene, IliBU, revealed that the lactolisterin BU cluster consists of four genes: the structural gene IliBU, the abcT gene encoding an ABC transporter, the accL gene encoding an accessory protein and the immL gene which provides limited immunity to lactolisterin BU. Reverse transcription analysis revealed that all genes were transcribed as one polycistronic mRNA. Attempts to split the lactolisterin BU operon, even when both parts were under control of the PlliBU promoter, were unsuccessful indicating that expression of lactolisterin BU is probably precisely regulated at the translational level by translational coupling and is possible only when all genes of the operon are in cis constellation. Two rho-independent transcription terminators were detected in the lactolisterin BU operon: the first in the intergenic region of the IliBU and abcT genes and the second at the end of operon. Deletion of the second transcription terminator did not influence production of the bacteriocin in lactococci.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Functional Characterization of the Lactolisterin BU Gene Cluster of Lactococcus lactis subsp. lactis BGBU1-4
VL  - 9
DO  - 10.3389/fmicb.2018.02774
ER  - 

@article{
author = "Miljković, Manja and Lozo, Jelena and Mirković, Nemanja and O'Connor, Paula M. and Malešević, Milka and Jovčić, Branko and Cotter, Paul D. and Kojić, Milan",
year = "2018",
abstract = "The gene cluster responsible for the production of the aureocin A53-like bacteriocin, lactolisterin BU, is located on plasmid pBU6 in Lactococcus lactis subsp. lactis BGBU1-4. Heterologous expression of pBU6 confirmed that production and limited immunity to lactolisterin BU were provided by the plasmid. Comparative analysis of aureocin A53-like operons revealed that the structural genes shared a low level of identity, while other genes were without homology, indicating a different origin. Subcloning and expression of genes located downstream of the structural gene, IliBU, revealed that the lactolisterin BU cluster consists of four genes: the structural gene IliBU, the abcT gene encoding an ABC transporter, the accL gene encoding an accessory protein and the immL gene which provides limited immunity to lactolisterin BU. Reverse transcription analysis revealed that all genes were transcribed as one polycistronic mRNA. Attempts to split the lactolisterin BU operon, even when both parts were under control of the PlliBU promoter, were unsuccessful indicating that expression of lactolisterin BU is probably precisely regulated at the translational level by translational coupling and is possible only when all genes of the operon are in cis constellation. Two rho-independent transcription terminators were detected in the lactolisterin BU operon: the first in the intergenic region of the IliBU and abcT genes and the second at the end of operon. Deletion of the second transcription terminator did not influence production of the bacteriocin in lactococci.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Functional Characterization of the Lactolisterin BU Gene Cluster of Lactococcus lactis subsp. lactis BGBU1-4",
volume = "9",
doi = "10.3389/fmicb.2018.02774"
}

Miljković, M., Lozo, J., Mirković, N., O'Connor, P. M., Malešević, M., Jovčić, B., Cotter, P. D.,& Kojić, M.. (2018). Functional Characterization of the Lactolisterin BU Gene Cluster of Lactococcus lactis subsp. lactis BGBU1-4. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fmicb.2018.02774

Miljković M, Lozo J, Mirković N, O'Connor PM, Malešević M, Jovčić B, Cotter PD, Kojić M. Functional Characterization of the Lactolisterin BU Gene Cluster of Lactococcus lactis subsp. lactis BGBU1-4. in Frontiers in Microbiology. 2018;9.
doi:10.3389/fmicb.2018.02774 .

Miljković, Manja, Lozo, Jelena, Mirković, Nemanja, O'Connor, Paula M., Malešević, Milka, Jovčić, Branko, Cotter, Paul D., Kojić, Milan, "Functional Characterization of the Lactolisterin BU Gene Cluster of Lactococcus lactis subsp. lactis BGBU1-4" in Frontiers in Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.02774 . .

TY  - JOUR
AU  - Lozo, Jelena
AU  - Mirković, Nemanja
AU  - O'Connor, Paula M.
AU  - Malešević, Milka
AU  - Miljković, Marija
AU  - Polović, Natalija
AU  - Jovčić, Branko
AU  - Cotter, Paul D.
AU  - Kojić, Milan
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1001
AB  - Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 produces a novel bacteriocin, lactolisterin BU, with strong antimicrobial activity against many species of Gram-positive bacteria, including important food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU was extracted from the cell surface of BGBU1-4 by 2-propanol and purified to homogeneity by C18 solid-phase extraction and reversed-phase high-performance liquid chromatography. The molecular mass of the purified lactolisterin BU was 5,160.94 Da, and an internal fragment, AVSWAWQH, as determined by N-terminal sequencing, showed low-level similarity to existing antimicrobial peptides. Curing and transformation experiments revealed the presence of a corresponding bacteriocin operon on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4. Analysis of the bacteriocin operon revealed a leaderless bacteriocin of 43 amino acids that exhibited similarity to bacteriocin BHT-B (63%) from Streptococcus ratti, a bacteriocin with analogy to aureocin A. IMPORTANCE Lactolisterin BU, a broad-spectrum leaderless bacteriocin produced by L. lactis subsp. lactis bv. diacetylactis BGBU1-4, expresses strong antimicrobial activity against food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU showed the highest similarity to aureocin-like bacteriocins produced by different bacteria. The operon for synthesis is located on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4, indicating possible horizontal transfer among producers.
PB  - Amer Soc Microbiology, Washington
T2  - Applied and Environmental Microbiology
T1  - Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4
IS  - 21
VL  - 83
DO  - 10.1128/AEM.01519-17
ER  - 

@article{
author = "Lozo, Jelena and Mirković, Nemanja and O'Connor, Paula M. and Malešević, Milka and Miljković, Marija and Polović, Natalija and Jovčić, Branko and Cotter, Paul D. and Kojić, Milan",
year = "2017",
abstract = "Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 produces a novel bacteriocin, lactolisterin BU, with strong antimicrobial activity against many species of Gram-positive bacteria, including important food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU was extracted from the cell surface of BGBU1-4 by 2-propanol and purified to homogeneity by C18 solid-phase extraction and reversed-phase high-performance liquid chromatography. The molecular mass of the purified lactolisterin BU was 5,160.94 Da, and an internal fragment, AVSWAWQH, as determined by N-terminal sequencing, showed low-level similarity to existing antimicrobial peptides. Curing and transformation experiments revealed the presence of a corresponding bacteriocin operon on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4. Analysis of the bacteriocin operon revealed a leaderless bacteriocin of 43 amino acids that exhibited similarity to bacteriocin BHT-B (63%) from Streptococcus ratti, a bacteriocin with analogy to aureocin A. IMPORTANCE Lactolisterin BU, a broad-spectrum leaderless bacteriocin produced by L. lactis subsp. lactis bv. diacetylactis BGBU1-4, expresses strong antimicrobial activity against food spoilage and foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, Bacillus spp., and streptococci. Lactolisterin BU showed the highest similarity to aureocin-like bacteriocins produced by different bacteria. The operon for synthesis is located on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4, indicating possible horizontal transfer among producers.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Applied and Environmental Microbiology",
title = "Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4",
number = "21",
volume = "83",
doi = "10.1128/AEM.01519-17"
}

Lozo, J., Mirković, N., O'Connor, P. M., Malešević, M., Miljković, M., Polović, N., Jovčić, B., Cotter, P. D.,& Kojić, M.. (2017). Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4. in Applied and Environmental Microbiology
Amer Soc Microbiology, Washington., 83(21).
https://doi.org/10.1128/AEM.01519-17

Lozo J, Mirković N, O'Connor PM, Malešević M, Miljković M, Polović N, Jovčić B, Cotter PD, Kojić M. Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4. in Applied and Environmental Microbiology. 2017;83(21).
doi:10.1128/AEM.01519-17 .

Lozo, Jelena, Mirković, Nemanja, O'Connor, Paula M., Malešević, Milka, Miljković, Marija, Polović, Natalija, Jovčić, Branko, Cotter, Paul D., Kojić, Milan, "Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp lactis bv. diacetylactis BGBU1-4" in Applied and Environmental Microbiology, 83, no. 21 (2017),
https://doi.org/10.1128/AEM.01519-17 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Jancić, I.
AU  - Mirković, N.
AU  - Bufan, B.
AU  - Đokić, Jelena
AU  - Milenković, M.
AU  - Begović, Jelena
AU  - Strahinić, Ivana
AU  - Lozo, Jelena
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1003
AB  - In the light of the increasing resistance of bacterial pathogens to antibiotics, one of the main global strategies in applied science is development of alternative treatments, which would be safe both for the host and from the environmental perspective. Accordingly, the aim of this study was to test whether two lactic acid bacteria (LAB) strains, Lactococcus lactis BGBU1-4 and Lactobacillus salivarius BGHO1, could be applied as safe supplements for Listeria infection. Two major research objectives were set: to compare the effects of BGBU1-4 and BGHO1 on early immune response in gut tissue of Wistar rats co-administered with Listeria monocytogenes ATCC19111 and next, to test how this applies to their usage as therapeutics in acute ATCC19111 infection. Intestinal villi (IV), Peyer's patches (PP) and mesenteric lymph nodes (MLN) were used for the analysis. The results showed that BGHO1 increased the mRNA expression of innate immune markers CD14, interleukin (IL)-1 beta and tumour necrosis factor (TNF)-alpha in PP and IV, and, in parallel, caused a decrease of listeriolysin O (LLO) mRNA expression in same tissues. In MLN of BGHO1 treated rats, LLO expression was increased, along with an increase of the expression of OX-62 mRNA and CD69, pointing to the activation of adaptive immunity. On the other hand, in BGBU1-4 treated rats, there was no reduction of LLO mRNA expression and no induction of innate immunity markers in intestinal tissue. Additionally, CD14 and IL-1 beta, as well as LLO, but not OX-62 mRNA and CD69 expression, were elevated in MLN of BGBU1-4 treated rats. However, when applied therapeutically, both, BGBU1-4 and BGHO1, lowered Listeria count in spleens of infected rats. Our results not only reveal the potential of LAB to ameliorate Listeria infections, but suggest different immunological effects of two different LAB strains, both of which could be effective in Listeria elimination.
PB  - Wageningen Academic Publishers, Wageningen
T2  - Beneficial Microbes
T1  - Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes
EP  - 822
IS  - 5
SP  - 809
VL  - 8
DO  - 10.3920/BM2017.0007
ER  - 

@article{
author = "Lukić, Jovanka and Jancić, I. and Mirković, N. and Bufan, B. and Đokić, Jelena and Milenković, M. and Begović, Jelena and Strahinić, Ivana and Lozo, Jelena",
year = "2017",
abstract = "In the light of the increasing resistance of bacterial pathogens to antibiotics, one of the main global strategies in applied science is development of alternative treatments, which would be safe both for the host and from the environmental perspective. Accordingly, the aim of this study was to test whether two lactic acid bacteria (LAB) strains, Lactococcus lactis BGBU1-4 and Lactobacillus salivarius BGHO1, could be applied as safe supplements for Listeria infection. Two major research objectives were set: to compare the effects of BGBU1-4 and BGHO1 on early immune response in gut tissue of Wistar rats co-administered with Listeria monocytogenes ATCC19111 and next, to test how this applies to their usage as therapeutics in acute ATCC19111 infection. Intestinal villi (IV), Peyer's patches (PP) and mesenteric lymph nodes (MLN) were used for the analysis. The results showed that BGHO1 increased the mRNA expression of innate immune markers CD14, interleukin (IL)-1 beta and tumour necrosis factor (TNF)-alpha in PP and IV, and, in parallel, caused a decrease of listeriolysin O (LLO) mRNA expression in same tissues. In MLN of BGHO1 treated rats, LLO expression was increased, along with an increase of the expression of OX-62 mRNA and CD69, pointing to the activation of adaptive immunity. On the other hand, in BGBU1-4 treated rats, there was no reduction of LLO mRNA expression and no induction of innate immunity markers in intestinal tissue. Additionally, CD14 and IL-1 beta, as well as LLO, but not OX-62 mRNA and CD69 expression, were elevated in MLN of BGBU1-4 treated rats. However, when applied therapeutically, both, BGBU1-4 and BGHO1, lowered Listeria count in spleens of infected rats. Our results not only reveal the potential of LAB to ameliorate Listeria infections, but suggest different immunological effects of two different LAB strains, both of which could be effective in Listeria elimination.",
publisher = "Wageningen Academic Publishers, Wageningen",
journal = "Beneficial Microbes",
title = "Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes",
pages = "822-809",
number = "5",
volume = "8",
doi = "10.3920/BM2017.0007"
}

Lukić, J., Jancić, I., Mirković, N., Bufan, B., Đokić, J., Milenković, M., Begović, J., Strahinić, I.,& Lozo, J.. (2017). Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes. in Beneficial Microbes
Wageningen Academic Publishers, Wageningen., 8(5), 809-822.
https://doi.org/10.3920/BM2017.0007

Lukić J, Jancić I, Mirković N, Bufan B, Đokić J, Milenković M, Begović J, Strahinić I, Lozo J. Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes. in Beneficial Microbes. 2017;8(5):809-822.
doi:10.3920/BM2017.0007 .

Lukić, Jovanka, Jancić, I., Mirković, N., Bufan, B., Đokić, Jelena, Milenković, M., Begović, Jelena, Strahinić, Ivana, Lozo, Jelena, "Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes" in Beneficial Microbes, 8, no. 5 (2017):809-822,
https://doi.org/10.3920/BM2017.0007 . .

TY  - JOUR
AU  - Biocanin, Marjan
AU  - Madi, Haowa
AU  - Vasiljević, Zorica
AU  - Kojić, Milan
AU  - Jovčić, Branko
AU  - Lozo, Jelena
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1020
AB  - Stenotrophomonas maltophilia, an opportunistic pathogen usually connected with healthcare-associated infections, is an environmental bacterium. Intrinsic resistance to multiple antibiotics, with different virulence determinants in the last decade classified this bacterium in the group of global multiple drug resistant (MDR) organism. S. maltophilia clinical isolates, were collected from tertiary care pediatric hospital in Belgrade, Serbia to investigate influence of different factors on biofilm formation, kinetics of biofilm formation for strong biofilm producers and effect of trimethoprim-sulfamethoxazole (TMP/SMX) on formed biofilm. Most of the isolates (89.8%) were able to form a biofilm. Analysis of biofilm formation in different growth conditions showed that changing of temeperature and pH had the stronggest effect on biofilm formation almost equally in group of cystic fibrosis (CF) and non-CF strains. TMP/SMX in concentration of 50 mu g/ml reduced completely 24 h old biofilms while concentration of 25 mu g/ml effects formed biofilms in a strain dependent manner. Among strains able to form strong biofilm CF isolates formed biofilm slower than non-CF isolates, while shaking conditions did not affect biofilm formation. Swimming motility was detected in both CF and non-CF isolates, however more motile strain formed stronger biofilms. This study suggests that temperature, pH and TMP/SMX had the strongest influence on biofilm formation in analyzed collection of S. maltophilia. A positive correlation between motility and strength of formed biofilm was demonstrated.
PB  - Polskie Towarzystwo Mikrobiologow-Polish Society Of Microbiologists, Warsaw
T2  - Polish Journal of Microbiology
T1  - Temperature, pH and Trimethoprim-Sulfamethoxazole Are Potent Inhibitors of Biofilm Formation by Stenotrophomonas maltophilia Clinical Isolates
EP  - 438
IS  - 4
SP  - 433
VL  - 66
DO  - 10.5604/01.3001.0010.6996
ER  - 

@article{
author = "Biocanin, Marjan and Madi, Haowa and Vasiljević, Zorica and Kojić, Milan and Jovčić, Branko and Lozo, Jelena",
year = "2017",
abstract = "Stenotrophomonas maltophilia, an opportunistic pathogen usually connected with healthcare-associated infections, is an environmental bacterium. Intrinsic resistance to multiple antibiotics, with different virulence determinants in the last decade classified this bacterium in the group of global multiple drug resistant (MDR) organism. S. maltophilia clinical isolates, were collected from tertiary care pediatric hospital in Belgrade, Serbia to investigate influence of different factors on biofilm formation, kinetics of biofilm formation for strong biofilm producers and effect of trimethoprim-sulfamethoxazole (TMP/SMX) on formed biofilm. Most of the isolates (89.8%) were able to form a biofilm. Analysis of biofilm formation in different growth conditions showed that changing of temeperature and pH had the stronggest effect on biofilm formation almost equally in group of cystic fibrosis (CF) and non-CF strains. TMP/SMX in concentration of 50 mu g/ml reduced completely 24 h old biofilms while concentration of 25 mu g/ml effects formed biofilms in a strain dependent manner. Among strains able to form strong biofilm CF isolates formed biofilm slower than non-CF isolates, while shaking conditions did not affect biofilm formation. Swimming motility was detected in both CF and non-CF isolates, however more motile strain formed stronger biofilms. This study suggests that temperature, pH and TMP/SMX had the strongest influence on biofilm formation in analyzed collection of S. maltophilia. A positive correlation between motility and strength of formed biofilm was demonstrated.",
publisher = "Polskie Towarzystwo Mikrobiologow-Polish Society Of Microbiologists, Warsaw",
journal = "Polish Journal of Microbiology",
title = "Temperature, pH and Trimethoprim-Sulfamethoxazole Are Potent Inhibitors of Biofilm Formation by Stenotrophomonas maltophilia Clinical Isolates",
pages = "438-433",
number = "4",
volume = "66",
doi = "10.5604/01.3001.0010.6996"
}

Biocanin, M., Madi, H., Vasiljević, Z., Kojić, M., Jovčić, B.,& Lozo, J.. (2017). Temperature, pH and Trimethoprim-Sulfamethoxazole Are Potent Inhibitors of Biofilm Formation by Stenotrophomonas maltophilia Clinical Isolates. in Polish Journal of Microbiology
Polskie Towarzystwo Mikrobiologow-Polish Society Of Microbiologists, Warsaw., 66(4), 433-438.
https://doi.org/10.5604/01.3001.0010.6996

Biocanin M, Madi H, Vasiljević Z, Kojić M, Jovčić B, Lozo J. Temperature, pH and Trimethoprim-Sulfamethoxazole Are Potent Inhibitors of Biofilm Formation by Stenotrophomonas maltophilia Clinical Isolates. in Polish Journal of Microbiology. 2017;66(4):433-438.
doi:10.5604/01.3001.0010.6996 .

Biocanin, Marjan, Madi, Haowa, Vasiljević, Zorica, Kojić, Milan, Jovčić, Branko, Lozo, Jelena, "Temperature, pH and Trimethoprim-Sulfamethoxazole Are Potent Inhibitors of Biofilm Formation by Stenotrophomonas maltophilia Clinical Isolates" in Polish Journal of Microbiology, 66, no. 4 (2017):433-438,
https://doi.org/10.5604/01.3001.0010.6996 . .

TY  - JOUR
AU  - Novović, Katarina
AU  - Vasiljević, Zorica
AU  - Kuzmanović, Milos
AU  - Lozo, Jelena
AU  - Begović, Jelena
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/957
AB  - New Delhi metallo--lactamase (NDM) is a serious challenge to the treatment of infections and public health. Serbia has been designated as an endemic region for isolates carrying the bla(NDM-1) gene, as well as one of several commonly proposed countries of origin. This is the first report of NDM-1-positive Escherichia coli from Serbia. A carbapenem-resistant clinical isolate of E. coli strain IMD989, isolated from the blood culture of a pediatric patient with leukemia, was subjected to antimicrobial susceptibility tests, molecular typing, and conjugation experiments. The strain exhibited resistance to meropenem and was classified as a novel sequence type, ST5123, belonging to E. coli phylogenetic group A. ST5123 showed similarity to veterinary isolates ST93 and ST3977. The bla(NDM-1) gene was detected by polymerase chain reaction (PCR) and sequencing. Cloning and sequencing of genomic clones confirmed that strain IMD989 produces an NDM-1 variant. Conjugation experiments, pulsed-field gel electrophoresis, and Southern blot hybridization revealed that bla(NDM-1) was located in IMD989 on a transmissible 80kb plasmid, designated as pIMD989. PCR analysis confirmed that pIMD989 belongs to the IncF plasmid family. Propagation of IMD989 and selected transconjugants carrying pIMD989 over 14 days in solid media with and without antibiotic selection showed that pIMD989 is a stable plasmid.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Microbial Drug Resistance
T1  - Novel E-coli ST5123 Containing bla(NDM-1) Carried by IncF Plasmid Isolated from a Pediatric Patient in Serbia
EP  - 711
IS  - 8
SP  - 707
VL  - 22
DO  - 10.1089/mdr.2015.0264
ER  - 

@article{
author = "Novović, Katarina and Vasiljević, Zorica and Kuzmanović, Milos and Lozo, Jelena and Begović, Jelena and Kojić, Milan and Jovčić, Branko",
year = "2016",
abstract = "New Delhi metallo--lactamase (NDM) is a serious challenge to the treatment of infections and public health. Serbia has been designated as an endemic region for isolates carrying the bla(NDM-1) gene, as well as one of several commonly proposed countries of origin. This is the first report of NDM-1-positive Escherichia coli from Serbia. A carbapenem-resistant clinical isolate of E. coli strain IMD989, isolated from the blood culture of a pediatric patient with leukemia, was subjected to antimicrobial susceptibility tests, molecular typing, and conjugation experiments. The strain exhibited resistance to meropenem and was classified as a novel sequence type, ST5123, belonging to E. coli phylogenetic group A. ST5123 showed similarity to veterinary isolates ST93 and ST3977. The bla(NDM-1) gene was detected by polymerase chain reaction (PCR) and sequencing. Cloning and sequencing of genomic clones confirmed that strain IMD989 produces an NDM-1 variant. Conjugation experiments, pulsed-field gel electrophoresis, and Southern blot hybridization revealed that bla(NDM-1) was located in IMD989 on a transmissible 80kb plasmid, designated as pIMD989. PCR analysis confirmed that pIMD989 belongs to the IncF plasmid family. Propagation of IMD989 and selected transconjugants carrying pIMD989 over 14 days in solid media with and without antibiotic selection showed that pIMD989 is a stable plasmid.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Microbial Drug Resistance",
title = "Novel E-coli ST5123 Containing bla(NDM-1) Carried by IncF Plasmid Isolated from a Pediatric Patient in Serbia",
pages = "711-707",
number = "8",
volume = "22",
doi = "10.1089/mdr.2015.0264"
}

Novović, K., Vasiljević, Z., Kuzmanović, M., Lozo, J., Begović, J., Kojić, M.,& Jovčić, B.. (2016). Novel E-coli ST5123 Containing bla(NDM-1) Carried by IncF Plasmid Isolated from a Pediatric Patient in Serbia. in Microbial Drug Resistance
Mary Ann Liebert, Inc, New Rochelle., 22(8), 707-711.
https://doi.org/10.1089/mdr.2015.0264

Novović K, Vasiljević Z, Kuzmanović M, Lozo J, Begović J, Kojić M, Jovčić B. Novel E-coli ST5123 Containing bla(NDM-1) Carried by IncF Plasmid Isolated from a Pediatric Patient in Serbia. in Microbial Drug Resistance. 2016;22(8):707-711.
doi:10.1089/mdr.2015.0264 .

Novović, Katarina, Vasiljević, Zorica, Kuzmanović, Milos, Lozo, Jelena, Begović, Jelena, Kojić, Milan, Jovčić, Branko, "Novel E-coli ST5123 Containing bla(NDM-1) Carried by IncF Plasmid Isolated from a Pediatric Patient in Serbia" in Microbial Drug Resistance, 22, no. 8 (2016):707-711,
https://doi.org/10.1089/mdr.2015.0264 . .

TY  - JOUR
AU  - Madi, Haowa
AU  - Lukić, Jovanka
AU  - Vasiljević, Zorica
AU  - Biocanin, Marjan
AU  - Kojić, Milan
AU  - Jovčić, Branko
AU  - Lozo, Jelena
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/923
AB  - Background Stenotrophomonas maltophilia is an environmental bacterium and an opportunistic pathogen usually associated with healthcare-associated infections, which has recently been recognized as a globally multi-drug resistant organism. The aim of this study was genotyping and physiological characterization of Stenotrophomonas maltophilia isolated in a large, tertiary care pediatric hospital in Belgrade, Serbia, hosting the national reference cystic fibrosis (CF) center for pediatric and adult patients. Methods We characterized 42 strains of cystic fibrosis (CF) and 46 strains of non-cystic fibrosis (non-CF) origin isolated from 2013 to 2015 in order to investigate their genetic relatedness and phenotypic traits. Genotyping was performed using sequencing of 16S rRNA gene, Pulse Field Gel Electrophoresis (PFGE) and Multi locus sequencing typing (MLST) analysis. Sensitivity to five relevant antimicrobial agents was determined, namely trimethoprim/sulfamethoxazole (TMP/SMX), chloramphenicol, ciprofloxacin, levofloxacin and tetracycline. Surface characteristics, motility, biofilm formation and adhesion to mucin were tested in all strains. Statistical approach was used to determine correlations between obtained results. Results Most of the isolates were not genetically related. Six new sequence types were determined. Strains were uniformly sensitive to all tested antimicrobial agents. The majority of isolates (89.8%) were able to form biofilm with almost equal representation in both CF and non-CF strains. Swimming motility was observed in all strains, while none of them exhibited swarming motility. Among strains able to adhere to mucin, no differences between CF and non-CF isolates were observed. Conclusions High genetic diversity among isolates implies the absence of clonal spread within the hospital. Positive correlation between motility, biofilm formation and adhesion to mucin was demonstrated. Biofilm formation and motility were more pronounced among non-CF than CF isolates.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Genotypic and Phenotypic Characterization of Stenotrophomonas maltophilia Strains from a Pediatric Tertiary Care Hospital in Serbia
IS  - 10
VL  - 11
DO  - 10.1371/journal.pone.0165660
ER  - 

@article{
author = "Madi, Haowa and Lukić, Jovanka and Vasiljević, Zorica and Biocanin, Marjan and Kojić, Milan and Jovčić, Branko and Lozo, Jelena",
year = "2016",
abstract = "Background Stenotrophomonas maltophilia is an environmental bacterium and an opportunistic pathogen usually associated with healthcare-associated infections, which has recently been recognized as a globally multi-drug resistant organism. The aim of this study was genotyping and physiological characterization of Stenotrophomonas maltophilia isolated in a large, tertiary care pediatric hospital in Belgrade, Serbia, hosting the national reference cystic fibrosis (CF) center for pediatric and adult patients. Methods We characterized 42 strains of cystic fibrosis (CF) and 46 strains of non-cystic fibrosis (non-CF) origin isolated from 2013 to 2015 in order to investigate their genetic relatedness and phenotypic traits. Genotyping was performed using sequencing of 16S rRNA gene, Pulse Field Gel Electrophoresis (PFGE) and Multi locus sequencing typing (MLST) analysis. Sensitivity to five relevant antimicrobial agents was determined, namely trimethoprim/sulfamethoxazole (TMP/SMX), chloramphenicol, ciprofloxacin, levofloxacin and tetracycline. Surface characteristics, motility, biofilm formation and adhesion to mucin were tested in all strains. Statistical approach was used to determine correlations between obtained results. Results Most of the isolates were not genetically related. Six new sequence types were determined. Strains were uniformly sensitive to all tested antimicrobial agents. The majority of isolates (89.8%) were able to form biofilm with almost equal representation in both CF and non-CF strains. Swimming motility was observed in all strains, while none of them exhibited swarming motility. Among strains able to adhere to mucin, no differences between CF and non-CF isolates were observed. Conclusions High genetic diversity among isolates implies the absence of clonal spread within the hospital. Positive correlation between motility, biofilm formation and adhesion to mucin was demonstrated. Biofilm formation and motility were more pronounced among non-CF than CF isolates.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Genotypic and Phenotypic Characterization of Stenotrophomonas maltophilia Strains from a Pediatric Tertiary Care Hospital in Serbia",
number = "10",
volume = "11",
doi = "10.1371/journal.pone.0165660"
}

Madi, H., Lukić, J., Vasiljević, Z., Biocanin, M., Kojić, M., Jovčić, B.,& Lozo, J.. (2016). Genotypic and Phenotypic Characterization of Stenotrophomonas maltophilia Strains from a Pediatric Tertiary Care Hospital in Serbia. in PLoS One
Public Library Science, San Francisco., 11(10).
https://doi.org/10.1371/journal.pone.0165660

Madi H, Lukić J, Vasiljević Z, Biocanin M, Kojić M, Jovčić B, Lozo J. Genotypic and Phenotypic Characterization of Stenotrophomonas maltophilia Strains from a Pediatric Tertiary Care Hospital in Serbia. in PLoS One. 2016;11(10).
doi:10.1371/journal.pone.0165660 .

Madi, Haowa, Lukić, Jovanka, Vasiljević, Zorica, Biocanin, Marjan, Kojić, Milan, Jovčić, Branko, Lozo, Jelena, "Genotypic and Phenotypic Characterization of Stenotrophomonas maltophilia Strains from a Pediatric Tertiary Care Hospital in Serbia" in PLoS One, 11, no. 10 (2016),
https://doi.org/10.1371/journal.pone.0165660 . .

TY  - JOUR
AU  - Cirković, Ivana
AU  - Bozić, Dragana D.
AU  - Draganić, Veselin
AU  - Lozo, Jelena
AU  - Berić, Tanja
AU  - Kojić, Milan
AU  - Arsić, Biljana
AU  - Garalejić, Eliana
AU  - Đukić, Slobodanka
AU  - Stanković, Slaviša
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/958
AB  - Background Coagulase negative staphylococci (CoNS) and Listeria monocytogenes have important roles in pathogenesis of various genital tract infections and fatal foetomaternal infections, respectively. The aim of our study was to investigate the inhibitory effects of two novel bacteriocins on biofilms of CoNS and L. monocytogenes genital isolates. Methods The effects of licheniocin 50.2 from Bacillus licheniformis VPS50.2 and crude extract of bacteriocins produced by Lactococcus lactis subsp. lactis biovar. diacetylactis BGBU1-4 (BGBU1-4 crude extract) were evaluated on biofilm formation and formed biofilms of eight CoNS (four S. epidermidis, two S. hominis, one S. lugdunensis and one S. haemolyticus) and 12 L. monocytogenes genital isolates. Results Licheniocin 50.2 and BGBU1-4 crude extract inhibited the growth of both CoNS and L. monocytogenes isolates, with MIC values in the range between 200-400 AU/ml for licheniocin 50.2 and 400-3200 AU/ml for BGBU1-4 crude extract. Subinhibitory concentrations (1/2 x and 1/4 x MIC) of licheniocin 50.2 inhibited biofilm formation by all CoNS isolates (p  lt  0.05, respectively), while BGBU1-4 crude extract inhibited biofilm formation by all L. monocytogenes isolates (p  lt  0.01 and p  lt  0.05, respectively). Both bacteriocins in concentrations of 100 AU/mL and 200 AU/mL reduced the amount of 24 h old CoNS and L. monocytogenes biofilms (p  lt  0.05, p  lt  0.01, p  lt  0.001). Conclusions This study suggests that novel bacteriocins have potential to be used for genital application, to prevent biofilm formation and/or to eradicate formed biofilms, and consequently reduce genital and neonatal infections by CoNS and L. monocytogenes.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates
IS  - 12
VL  - 11
DO  - 10.1371/journal.pone.0167995
ER  - 

@article{
author = "Cirković, Ivana and Bozić, Dragana D. and Draganić, Veselin and Lozo, Jelena and Berić, Tanja and Kojić, Milan and Arsić, Biljana and Garalejić, Eliana and Đukić, Slobodanka and Stanković, Slaviša",
year = "2016",
abstract = "Background Coagulase negative staphylococci (CoNS) and Listeria monocytogenes have important roles in pathogenesis of various genital tract infections and fatal foetomaternal infections, respectively. The aim of our study was to investigate the inhibitory effects of two novel bacteriocins on biofilms of CoNS and L. monocytogenes genital isolates. Methods The effects of licheniocin 50.2 from Bacillus licheniformis VPS50.2 and crude extract of bacteriocins produced by Lactococcus lactis subsp. lactis biovar. diacetylactis BGBU1-4 (BGBU1-4 crude extract) were evaluated on biofilm formation and formed biofilms of eight CoNS (four S. epidermidis, two S. hominis, one S. lugdunensis and one S. haemolyticus) and 12 L. monocytogenes genital isolates. Results Licheniocin 50.2 and BGBU1-4 crude extract inhibited the growth of both CoNS and L. monocytogenes isolates, with MIC values in the range between 200-400 AU/ml for licheniocin 50.2 and 400-3200 AU/ml for BGBU1-4 crude extract. Subinhibitory concentrations (1/2 x and 1/4 x MIC) of licheniocin 50.2 inhibited biofilm formation by all CoNS isolates (p  lt  0.05, respectively), while BGBU1-4 crude extract inhibited biofilm formation by all L. monocytogenes isolates (p  lt  0.01 and p  lt  0.05, respectively). Both bacteriocins in concentrations of 100 AU/mL and 200 AU/mL reduced the amount of 24 h old CoNS and L. monocytogenes biofilms (p  lt  0.05, p  lt  0.01, p  lt  0.001). Conclusions This study suggests that novel bacteriocins have potential to be used for genital application, to prevent biofilm formation and/or to eradicate formed biofilms, and consequently reduce genital and neonatal infections by CoNS and L. monocytogenes.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates",
number = "12",
volume = "11",
doi = "10.1371/journal.pone.0167995"
}

Cirković, I., Bozić, D. D., Draganić, V., Lozo, J., Berić, T., Kojić, M., Arsić, B., Garalejić, E., Đukić, S.,& Stanković, S.. (2016). Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates. in PLoS One
Public Library Science, San Francisco., 11(12).
https://doi.org/10.1371/journal.pone.0167995

Cirković I, Bozić DD, Draganić V, Lozo J, Berić T, Kojić M, Arsić B, Garalejić E, Đukić S, Stanković S. Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates. in PLoS One. 2016;11(12).
doi:10.1371/journal.pone.0167995 .

Cirković, Ivana, Bozić, Dragana D., Draganić, Veselin, Lozo, Jelena, Berić, Tanja, Kojić, Milan, Arsić, Biljana, Garalejić, Eliana, Đukić, Slobodanka, Stanković, Slaviša, "Licheniocin 50.2 and Bacteriocins from Lactococcus lactis subsp lactis biovar. diacetylactis BGBU1-4 Inhibit Biofilms of Coagulase Negative Staphylococci and Listeria monocytogenes Clinical Isolates" in PLoS One, 11, no. 12 (2016),
https://doi.org/10.1371/journal.pone.0167995 . .

TY  - JOUR
AU  - Mirković, Nemanja
AU  - Radulović, Zorica
AU  - Uzelac, Gordana
AU  - Lozo, Jelena
AU  - Obradović, Dragojlo
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/870
AB  - Lactococcus locus ssp. lactis BGBM50, a producer of lactococcin G and aggregation-promoting factor, was isolated from selected lactic acid bacteria taken from semi-hard cheese traditionally produced in the village Zanjic, Montenegro. Strain BGBM50 harbours a number of plasmids of different sizes. Plasmid curing experiments showed that genes for bacteriocin production are located on pBM140, a plasmid 140 kb in length. PCR analysis with primers specific for lactococcin Q and G genes gave fragment of the expected size. In addition, after plasmid curing of strain BGBM50, different derivatives with altered phenotypes were obtained, among them BGBM50-34 strain, which retained bacteriocin synthesis but had enhanced aggregation ability.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain
EP  - 242
IS  - 2
SP  - 237
VL  - 53
DO  - 10.17113/ftb.53.02.15.3846
ER  - 

@article{
author = "Mirković, Nemanja and Radulović, Zorica and Uzelac, Gordana and Lozo, Jelena and Obradović, Dragojlo and Topisirović, Ljubiša and Kojić, Milan",
year = "2015",
abstract = "Lactococcus locus ssp. lactis BGBM50, a producer of lactococcin G and aggregation-promoting factor, was isolated from selected lactic acid bacteria taken from semi-hard cheese traditionally produced in the village Zanjic, Montenegro. Strain BGBM50 harbours a number of plasmids of different sizes. Plasmid curing experiments showed that genes for bacteriocin production are located on pBM140, a plasmid 140 kb in length. PCR analysis with primers specific for lactococcin Q and G genes gave fragment of the expected size. In addition, after plasmid curing of strain BGBM50, different derivatives with altered phenotypes were obtained, among them BGBM50-34 strain, which retained bacteriocin synthesis but had enhanced aggregation ability.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain",
pages = "242-237",
number = "2",
volume = "53",
doi = "10.17113/ftb.53.02.15.3846"
}

Mirković, N., Radulović, Z., Uzelac, G., Lozo, J., Obradović, D., Topisirović, L.,& Kojić, M.. (2015). Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain. in Food Technology and Biotechnology
University of Zagreb., 53(2), 237-242.
https://doi.org/10.17113/ftb.53.02.15.3846

Mirković N, Radulović Z, Uzelac G, Lozo J, Obradović D, Topisirović L, Kojić M. Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain. in Food Technology and Biotechnology. 2015;53(2):237-242.
doi:10.17113/ftb.53.02.15.3846 .

Mirković, Nemanja, Radulović, Zorica, Uzelac, Gordana, Lozo, Jelena, Obradović, Dragojlo, Topisirović, Ljubiša, Kojić, Milan, "Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp lactis BGBM50 Strain" in Food Technology and Biotechnology, 53, no. 2 (2015):237-242,
https://doi.org/10.17113/ftb.53.02.15.3846 . .

TY  - JOUR
AU  - Uzelac, Gordana
AU  - Miljković, Marija
AU  - Lozo, Jelena
AU  - Radulović, Zorica
AU  - Tošić, Nataša
AU  - Kojić, Milan
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/829
AB  - The production of LsbB, leaderless class II bacteriocin, is encoded by genes (lsbB and lmrB) located on plasmid pMN5 in Lactococcus lactis BGMN1-5. Heterologous expression of the lsbB gene using the pAZIL vector (pAZIL-lsbB) in L. lactis subsp. cremoris MG7284 resulted in a significant reduction (more than 30 times) of bacteriocin LsbB expression. Subcloning and deletion experiments with plasmid pMN5 revealed that full expression of LsbB requires the presence of a complete transcription terminator located downstream of the lsbB gene. RNA stability analysis revealed that the presence of a transcription terminator increased the RNA stability by three times and the expression of LsbB by 30 times. The study of the influence of transcription terminator on the expression of other bacteriocin genes (lcnB, for lactococcin B production) indicated that this translational terminator likely functions in a lsbB-specific manner rather than in a general manner.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Microbiological Research
T1  - Expression of bacteriocin LsbB is dependent on a transcription terminator
EP  - 53
SP  - 45
VL  - 179
DO  - 10.1016/j.micres.2015.06.011
ER  - 

@article{
author = "Uzelac, Gordana and Miljković, Marija and Lozo, Jelena and Radulović, Zorica and Tošić, Nataša and Kojić, Milan",
year = "2015",
abstract = "The production of LsbB, leaderless class II bacteriocin, is encoded by genes (lsbB and lmrB) located on plasmid pMN5 in Lactococcus lactis BGMN1-5. Heterologous expression of the lsbB gene using the pAZIL vector (pAZIL-lsbB) in L. lactis subsp. cremoris MG7284 resulted in a significant reduction (more than 30 times) of bacteriocin LsbB expression. Subcloning and deletion experiments with plasmid pMN5 revealed that full expression of LsbB requires the presence of a complete transcription terminator located downstream of the lsbB gene. RNA stability analysis revealed that the presence of a transcription terminator increased the RNA stability by three times and the expression of LsbB by 30 times. The study of the influence of transcription terminator on the expression of other bacteriocin genes (lcnB, for lactococcin B production) indicated that this translational terminator likely functions in a lsbB-specific manner rather than in a general manner.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Microbiological Research",
title = "Expression of bacteriocin LsbB is dependent on a transcription terminator",
pages = "53-45",
volume = "179",
doi = "10.1016/j.micres.2015.06.011"
}

Uzelac, G., Miljković, M., Lozo, J., Radulović, Z., Tošić, N.,& Kojić, M.. (2015). Expression of bacteriocin LsbB is dependent on a transcription terminator. in Microbiological Research
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 179, 45-53.
https://doi.org/10.1016/j.micres.2015.06.011

Uzelac G, Miljković M, Lozo J, Radulović Z, Tošić N, Kojić M. Expression of bacteriocin LsbB is dependent on a transcription terminator. in Microbiological Research. 2015;179:45-53.
doi:10.1016/j.micres.2015.06.011 .

Uzelac, Gordana, Miljković, Marija, Lozo, Jelena, Radulović, Zorica, Tošić, Nataša, Kojić, Milan, "Expression of bacteriocin LsbB is dependent on a transcription terminator" in Microbiological Research, 179 (2015):45-53,
https://doi.org/10.1016/j.micres.2015.06.011 . .

TY  - JOUR
AU  - Lozo, Jelena
AU  - Berić, Tanja
AU  - Terzić-Vidojević, Amarela
AU  - Stanković, Slaviša
AU  - Fira, Đorđe
AU  - Stanisavljević, L.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/817
AB  - Using cultivation-dependant method, we isolated 184 strains from fresh and old bee bread, pollen, larvae and adults of solitary bee Osmia cornuta. The 16S rDNA sequencing of 79 selected isolates gave the final species-specific identification of strains. Phylogenetic analysis indicated that microbiota isolated from five different sources were represented with 29 species within three different phyla, Firmicutes with 25 species, Actinobacteria with only one species and Proteobacteria with three species of Enterobacteriaceae. Bacterial biodiversity presented with Shannon-Wiener index (H') was highest in the alimentary tract of adults and old bee bread (H' = 2.43 and H' = 2.53, respectively) and in the same time no dominance of any species was scored. On the contrary, results obtained for Simpson index (D) showed that in pollen samples the dominant species was Pantoea agglomerans (D = 0.42) while in fresh bee bread that was Staphylococcus sp. (D = 0.27). We assume that microbial diversity detected in the tested samples of solitary bee O. cornuta probably come from environment.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Microbiota associated with pollen, bee bread, larvae and adults of solitary bee Osmia cornuta (Hymenoptera: Megachilidae)
EP  - 476
IS  - 4
SP  - 470
VL  - 105
DO  - 10.1017/S0007485315000292
ER  - 

@article{
author = "Lozo, Jelena and Berić, Tanja and Terzić-Vidojević, Amarela and Stanković, Slaviša and Fira, Đorđe and Stanisavljević, L.",
year = "2015",
abstract = "Using cultivation-dependant method, we isolated 184 strains from fresh and old bee bread, pollen, larvae and adults of solitary bee Osmia cornuta. The 16S rDNA sequencing of 79 selected isolates gave the final species-specific identification of strains. Phylogenetic analysis indicated that microbiota isolated from five different sources were represented with 29 species within three different phyla, Firmicutes with 25 species, Actinobacteria with only one species and Proteobacteria with three species of Enterobacteriaceae. Bacterial biodiversity presented with Shannon-Wiener index (H') was highest in the alimentary tract of adults and old bee bread (H' = 2.43 and H' = 2.53, respectively) and in the same time no dominance of any species was scored. On the contrary, results obtained for Simpson index (D) showed that in pollen samples the dominant species was Pantoea agglomerans (D = 0.42) while in fresh bee bread that was Staphylococcus sp. (D = 0.27). We assume that microbial diversity detected in the tested samples of solitary bee O. cornuta probably come from environment.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Microbiota associated with pollen, bee bread, larvae and adults of solitary bee Osmia cornuta (Hymenoptera: Megachilidae)",
pages = "476-470",
number = "4",
volume = "105",
doi = "10.1017/S0007485315000292"
}

Lozo, J., Berić, T., Terzić-Vidojević, A., Stanković, S., Fira, Đ.,& Stanisavljević, L.. (2015). Microbiota associated with pollen, bee bread, larvae and adults of solitary bee Osmia cornuta (Hymenoptera: Megachilidae). in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 105(4), 470-476.
https://doi.org/10.1017/S0007485315000292

Lozo J, Berić T, Terzić-Vidojević A, Stanković S, Fira Đ, Stanisavljević L. Microbiota associated with pollen, bee bread, larvae and adults of solitary bee Osmia cornuta (Hymenoptera: Megachilidae). in Bulletin of Entomological Research. 2015;105(4):470-476.
doi:10.1017/S0007485315000292 .

Lozo, Jelena, Berić, Tanja, Terzić-Vidojević, Amarela, Stanković, Slaviša, Fira, Đorđe, Stanisavljević, L., "Microbiota associated with pollen, bee bread, larvae and adults of solitary bee Osmia cornuta (Hymenoptera: Megachilidae)" in Bulletin of Entomological Research, 105, no. 4 (2015):470-476,
https://doi.org/10.1017/S0007485315000292 . .

TY  - JOUR
AU  - Berić, Tanja
AU  - Stanković, Slaviša
AU  - Draganić, V.
AU  - Kojić, Milan
AU  - Lozo, Jelena
AU  - Fira, Đorđe
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/792
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Novel antilisterial bacteriocin licheniocin 50.2 from Bacillus licheniformis VPS50.2 isolated from soil sample
EP  - 510
IS  - 3
SP  - 502
VL  - 116
DO  - 10.1111/jam.12393
ER  - 

@article{
author = "Berić, Tanja and Stanković, Slaviša and Draganić, V. and Kojić, Milan and Lozo, Jelena and Fira, Đorđe",
year = "2014",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Novel antilisterial bacteriocin licheniocin 50.2 from Bacillus licheniformis VPS50.2 isolated from soil sample",
pages = "510-502",
number = "3",
volume = "116",
doi = "10.1111/jam.12393"
}

Berić, T., Stanković, S., Draganić, V., Kojić, M., Lozo, J.,& Fira, Đ.. (2014). Novel antilisterial bacteriocin licheniocin 50.2 from Bacillus licheniformis VPS50.2 isolated from soil sample. in Journal of Applied Microbiology
Wiley, Hoboken., 116(3), 502-510.
https://doi.org/10.1111/jam.12393

Berić T, Stanković S, Draganić V, Kojić M, Lozo J, Fira Đ. Novel antilisterial bacteriocin licheniocin 50.2 from Bacillus licheniformis VPS50.2 isolated from soil sample. in Journal of Applied Microbiology. 2014;116(3):502-510.
doi:10.1111/jam.12393 .

Berić, Tanja, Stanković, Slaviša, Draganić, V., Kojić, Milan, Lozo, Jelena, Fira, Đorđe, "Novel antilisterial bacteriocin licheniocin 50.2 from Bacillus licheniformis VPS50.2 isolated from soil sample" in Journal of Applied Microbiology, 116, no. 3 (2014):502-510,
https://doi.org/10.1111/jam.12393 . .

TY  - JOUR
AU  - Uzelac, Gordana
AU  - Kojić, Milan
AU  - Lozo, Jelena
AU  - Aleksandrzak-Piekarczyk, Tamara
AU  - Gabrielsen, Christina
AU  - Kristensen, Tom
AU  - Nes, Ingolf F.
AU  - Diep, Dzung B.
AU  - Topisirović, Ljubiša
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/703
AB  - Lactococcus lactis subsp. lactis BGMN1-5 produces a leaderless class II bacteriocin called LsbB. To identify the receptor for LsbB, a cosmid library of the LsbB-sensitive strain BGMN1-596 was constructed. About 150 cosmid clones were individually isolated and transferred to LsbB-resistant mutants of BGMN1-596. Cosmid pAZILcos/MN2, carrying a 40-kb insert, was found to restore LsbB sensitivity in LsbB-resistant mutants. Further subcloning revealed that a 1.9-kb fragment, containing only one open reading frame, was sufficient to restore sensitivity. The fragment contains the gene yvjB coding for a Zn-dependent membrane-bound metallopeptidase, suggesting that this gene may serve as the receptor for LsbB. Further support for this notion derives from several independent experiments: (i) whole-genome sequencing confirmed that all LsbB-resistant mutants contain mutations in yvjB; (ii) disruption of yvjB by direct gene knockout rendered sensitive strains BGMN1-596 and IL1403 resistant to LsbB; and (iii) most compellingly, heterologous expression of yvjB in naturally resistant strains of other species, such as Lactobacillus paracasei and Enterococcus faecalis, also rendered them sensitive to the bacteriocin. To our knowledge, this is the first time a membrane-bound peptidase gene has been shown to be involved in bacteriocin sensitivity in target cells. We also demonstrated a novel successful approach for identifying bacteriocin receptors.
PB  - Amer Soc Microbiology, Washington
T2  - Journal of Bacteriology
T1  - A Zn-Dependent Metallopeptidase Is Responsible for Sensitivity to LsbB, a Class II Leaderless Bacteriocin of Lactococcus lactis subsp lactis BGMN1-5
EP  - 5621
IS  - 24
SP  - 5614
VL  - 195
DO  - 10.1128/JB.00859-13
ER  - 

@article{
author = "Uzelac, Gordana and Kojić, Milan and Lozo, Jelena and Aleksandrzak-Piekarczyk, Tamara and Gabrielsen, Christina and Kristensen, Tom and Nes, Ingolf F. and Diep, Dzung B. and Topisirović, Ljubiša",
year = "2013",
abstract = "Lactococcus lactis subsp. lactis BGMN1-5 produces a leaderless class II bacteriocin called LsbB. To identify the receptor for LsbB, a cosmid library of the LsbB-sensitive strain BGMN1-596 was constructed. About 150 cosmid clones were individually isolated and transferred to LsbB-resistant mutants of BGMN1-596. Cosmid pAZILcos/MN2, carrying a 40-kb insert, was found to restore LsbB sensitivity in LsbB-resistant mutants. Further subcloning revealed that a 1.9-kb fragment, containing only one open reading frame, was sufficient to restore sensitivity. The fragment contains the gene yvjB coding for a Zn-dependent membrane-bound metallopeptidase, suggesting that this gene may serve as the receptor for LsbB. Further support for this notion derives from several independent experiments: (i) whole-genome sequencing confirmed that all LsbB-resistant mutants contain mutations in yvjB; (ii) disruption of yvjB by direct gene knockout rendered sensitive strains BGMN1-596 and IL1403 resistant to LsbB; and (iii) most compellingly, heterologous expression of yvjB in naturally resistant strains of other species, such as Lactobacillus paracasei and Enterococcus faecalis, also rendered them sensitive to the bacteriocin. To our knowledge, this is the first time a membrane-bound peptidase gene has been shown to be involved in bacteriocin sensitivity in target cells. We also demonstrated a novel successful approach for identifying bacteriocin receptors.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Journal of Bacteriology",
title = "A Zn-Dependent Metallopeptidase Is Responsible for Sensitivity to LsbB, a Class II Leaderless Bacteriocin of Lactococcus lactis subsp lactis BGMN1-5",
pages = "5621-5614",
number = "24",
volume = "195",
doi = "10.1128/JB.00859-13"
}

Uzelac, G., Kojić, M., Lozo, J., Aleksandrzak-Piekarczyk, T., Gabrielsen, C., Kristensen, T., Nes, I. F., Diep, D. B.,& Topisirović, L.. (2013). A Zn-Dependent Metallopeptidase Is Responsible for Sensitivity to LsbB, a Class II Leaderless Bacteriocin of Lactococcus lactis subsp lactis BGMN1-5. in Journal of Bacteriology
Amer Soc Microbiology, Washington., 195(24), 5614-5621.
https://doi.org/10.1128/JB.00859-13

Uzelac G, Kojić M, Lozo J, Aleksandrzak-Piekarczyk T, Gabrielsen C, Kristensen T, Nes IF, Diep DB, Topisirović L. A Zn-Dependent Metallopeptidase Is Responsible for Sensitivity to LsbB, a Class II Leaderless Bacteriocin of Lactococcus lactis subsp lactis BGMN1-5. in Journal of Bacteriology. 2013;195(24):5614-5621.
doi:10.1128/JB.00859-13 .

Uzelac, Gordana, Kojić, Milan, Lozo, Jelena, Aleksandrzak-Piekarczyk, Tamara, Gabrielsen, Christina, Kristensen, Tom, Nes, Ingolf F., Diep, Dzung B., Topisirović, Ljubiša, "A Zn-Dependent Metallopeptidase Is Responsible for Sensitivity to LsbB, a Class II Leaderless Bacteriocin of Lactococcus lactis subsp lactis BGMN1-5" in Journal of Bacteriology, 195, no. 24 (2013):5614-5621,
https://doi.org/10.1128/JB.00859-13 . .

TY  - JOUR
AU  - Golić, Nataša
AU  - Cadez, Neza
AU  - Terzić-Vidojević, Amarela
AU  - Suranska, Hana
AU  - Beganović, Jasna
AU  - Lozo, Jelena
AU  - Kos, Blazenka
AU  - Susković, Jagoda
AU  - Raspor, Peter
AU  - Topisirović, Ljubiša
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/614
AB  - The goal of this study was the characterisation of indigenous lactic acid bacteria (LAB) and yeasts isolated from nine white pickled (BG) and nine fresh soft (ZG) artisanal cheeses collected in Serbia and Croatia. While LAB were present in all of the cheeses collected, yeasts were found in all BG cheeses but only in three ZG cheese samples. High LAB and yeast species diversity was determined (average H-L' = 0.4 and H-Y' = 0.8, respectively). The predominant LAB species in white pickled (BC) cheeses were Lactococcus lactis, Lactobacillus plantarum, and Leuconostoc mesenteroides, while in fresh soft (ZG) cheeses the most dominant LAB species were L. lactis, Enterococcus faecalis, and Leuconostoc pseudomesenteroides. Among the 20 yeast species found, Debaryomyces hansenii, Candida zeylanoides, and Torulaspora delbrueckii were found to be predominant in BC cheeses, while Yarrowia lipolytica was predominant in ZG cheeses. The characterisation of metabolic and technological potentials revealed that 53.4% of LAB isolates produced antimicrobial compounds, 44.3% of LAB strains showed proteolytic activity, while most of the yeast species possessed either lipolytic or proteolytic activity. In conclusion, the results obtained in this study showed that the composition of LAB and yeast populations in white pickled and fresh soft cheeses is region specific. The knowledge gained in this study could eventually be used to select region specific LAB and yeast strains for the production of white pickled and fresh soft artisanal cheeses with geographically specific origins under controlled conditions.
PB  - Elsevier Science Bv, Amsterdam
T2  - International Journal of Food Microbiology
T1  - Evaluation of lactic acid bacteria and yeast diversity in traditional white pickled and fresh soft cheeses from the mountain regions of Serbia and lowland regions of Croatia
EP  - 300
IS  - 2
SP  - 294
VL  - 166
DO  - 10.1016/j.ijfoodmicro.2013.05.032
ER  - 

@article{
author = "Golić, Nataša and Cadez, Neza and Terzić-Vidojević, Amarela and Suranska, Hana and Beganović, Jasna and Lozo, Jelena and Kos, Blazenka and Susković, Jagoda and Raspor, Peter and Topisirović, Ljubiša",
year = "2013",
abstract = "The goal of this study was the characterisation of indigenous lactic acid bacteria (LAB) and yeasts isolated from nine white pickled (BG) and nine fresh soft (ZG) artisanal cheeses collected in Serbia and Croatia. While LAB were present in all of the cheeses collected, yeasts were found in all BG cheeses but only in three ZG cheese samples. High LAB and yeast species diversity was determined (average H-L' = 0.4 and H-Y' = 0.8, respectively). The predominant LAB species in white pickled (BC) cheeses were Lactococcus lactis, Lactobacillus plantarum, and Leuconostoc mesenteroides, while in fresh soft (ZG) cheeses the most dominant LAB species were L. lactis, Enterococcus faecalis, and Leuconostoc pseudomesenteroides. Among the 20 yeast species found, Debaryomyces hansenii, Candida zeylanoides, and Torulaspora delbrueckii were found to be predominant in BC cheeses, while Yarrowia lipolytica was predominant in ZG cheeses. The characterisation of metabolic and technological potentials revealed that 53.4% of LAB isolates produced antimicrobial compounds, 44.3% of LAB strains showed proteolytic activity, while most of the yeast species possessed either lipolytic or proteolytic activity. In conclusion, the results obtained in this study showed that the composition of LAB and yeast populations in white pickled and fresh soft cheeses is region specific. The knowledge gained in this study could eventually be used to select region specific LAB and yeast strains for the production of white pickled and fresh soft artisanal cheeses with geographically specific origins under controlled conditions.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Food Microbiology",
title = "Evaluation of lactic acid bacteria and yeast diversity in traditional white pickled and fresh soft cheeses from the mountain regions of Serbia and lowland regions of Croatia",
pages = "300-294",
number = "2",
volume = "166",
doi = "10.1016/j.ijfoodmicro.2013.05.032"
}

Golić, N., Cadez, N., Terzić-Vidojević, A., Suranska, H., Beganović, J., Lozo, J., Kos, B., Susković, J., Raspor, P.,& Topisirović, L.. (2013). Evaluation of lactic acid bacteria and yeast diversity in traditional white pickled and fresh soft cheeses from the mountain regions of Serbia and lowland regions of Croatia. in International Journal of Food Microbiology
Elsevier Science Bv, Amsterdam., 166(2), 294-300.
https://doi.org/10.1016/j.ijfoodmicro.2013.05.032

Golić N, Cadez N, Terzić-Vidojević A, Suranska H, Beganović J, Lozo J, Kos B, Susković J, Raspor P, Topisirović L. Evaluation of lactic acid bacteria and yeast diversity in traditional white pickled and fresh soft cheeses from the mountain regions of Serbia and lowland regions of Croatia. in International Journal of Food Microbiology. 2013;166(2):294-300.
doi:10.1016/j.ijfoodmicro.2013.05.032 .

Golić, Nataša, Cadez, Neza, Terzić-Vidojević, Amarela, Suranska, Hana, Beganović, Jasna, Lozo, Jelena, Kos, Blazenka, Susković, Jagoda, Raspor, Peter, Topisirović, Ljubiša, "Evaluation of lactic acid bacteria and yeast diversity in traditional white pickled and fresh soft cheeses from the mountain regions of Serbia and lowland regions of Croatia" in International Journal of Food Microbiology, 166, no. 2 (2013):294-300,
https://doi.org/10.1016/j.ijfoodmicro.2013.05.032 . .

TY  - JOUR
AU  - Strahinić, Ivana
AU  - Lozo, Jelena
AU  - Terzić-Vidojević, Amarela
AU  - Fira, Đorđe
AU  - Kojić, Milan
AU  - Golić, Nataša
AU  - Begović, Jelena
AU  - Topisirović, Ljubiša
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/632
AB  - Lactobacillus helveticus BGRA43 is a human intestinal isolate showing antimicrobial activity, amongst others, against Yersinia enterocolitica, Shigella sonnei, Shigella flexneri, and Streptococcus pneumoniae. BGRA43 produces PrtH proteinase with proteolytic activity on both casein and beta-lactoglobulin (BLG). BGRA43 is able to reduce the allergenicity of BLG. Bioactive peptides released in BGRA43 fermented milk are potent modulators of innate immunity by modulating the production of proinflammatory cytokines IL-6 and TNF-alpha. BGRA43 is able to survive in simulated gastric and intestinal conditions. The growth of BGRA43 in milk results in a fast acidification lowering the milk pH to 4.53 generating mild, homogeneous, and viscous yogurt-like product. The strain BGRA43 grows suitably in pure cow or goat's milk as well as in milk containing inulin or nutrim even when they are used as the sole carbon source. It is suggested that strain BGRA43 could be used as a single-strain culture for the preparation of yogurt-like products from bovine or caprine milk. Overall, L. helveticus BGRA43 could be considered as a potential probiotic candidate with appropriate technological properties attractive for the dairy industry.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus
VL  - 4
DO  - 10.3389/fmicb.2013.00002
ER  - 

@article{
author = "Strahinić, Ivana and Lozo, Jelena and Terzić-Vidojević, Amarela and Fira, Đorđe and Kojić, Milan and Golić, Nataša and Begović, Jelena and Topisirović, Ljubiša",
year = "2013",
abstract = "Lactobacillus helveticus BGRA43 is a human intestinal isolate showing antimicrobial activity, amongst others, against Yersinia enterocolitica, Shigella sonnei, Shigella flexneri, and Streptococcus pneumoniae. BGRA43 produces PrtH proteinase with proteolytic activity on both casein and beta-lactoglobulin (BLG). BGRA43 is able to reduce the allergenicity of BLG. Bioactive peptides released in BGRA43 fermented milk are potent modulators of innate immunity by modulating the production of proinflammatory cytokines IL-6 and TNF-alpha. BGRA43 is able to survive in simulated gastric and intestinal conditions. The growth of BGRA43 in milk results in a fast acidification lowering the milk pH to 4.53 generating mild, homogeneous, and viscous yogurt-like product. The strain BGRA43 grows suitably in pure cow or goat's milk as well as in milk containing inulin or nutrim even when they are used as the sole carbon source. It is suggested that strain BGRA43 could be used as a single-strain culture for the preparation of yogurt-like products from bovine or caprine milk. Overall, L. helveticus BGRA43 could be considered as a potential probiotic candidate with appropriate technological properties attractive for the dairy industry.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus",
volume = "4",
doi = "10.3389/fmicb.2013.00002"
}

Strahinić, I., Lozo, J., Terzić-Vidojević, A., Fira, Đ., Kojić, M., Golić, N., Begović, J.,& Topisirović, L.. (2013). Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 4.
https://doi.org/10.3389/fmicb.2013.00002

Strahinić I, Lozo J, Terzić-Vidojević A, Fira Đ, Kojić M, Golić N, Begović J, Topisirović L. Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus. in Frontiers in Microbiology. 2013;4.
doi:10.3389/fmicb.2013.00002 .

Strahinić, Ivana, Lozo, Jelena, Terzić-Vidojević, Amarela, Fira, Đorđe, Kojić, Milan, Golić, Nataša, Begović, Jelena, Topisirović, Ljubiša, "Technological and probiotic potential of BGRA43 a natural isolate of Lactobacillus helveticus" in Frontiers in Microbiology, 4 (2013),
https://doi.org/10.3389/fmicb.2013.00002 . .

TY  - JOUR
AU  - Strahinić, Ivana
AU  - Lukić, Jovanka
AU  - Terzić-Vidojević, Amarela
AU  - Lozo, Jelena
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/669
AB  - Lactobacillus helveticus BGRA43 isolated from human intestines shows antimicrobial activity against foodborne pathogens and during fermentation in milk releases peptides with demonstrated anti-inflammatory properties. In this study, it was found that strain BGRA43 exhibits antimicrobial activity against human pathogens Yersinia enterocolitica, Shigella sonnei, S. flexneri and Streptococcus pneumoniae. Strain BGRA43 was able to survive in simulated gastric juice containing milk and retained cell number stability during the incubation in simulated intestinal conditions. In addition, LC/MS/MS analysis showed the ability of BGRA43 to hydrolyze beta-lactoglobulin. Abundant growth of strain BGRA43 occurred in the presence of prebiotics inulin or concentrated oat bran beta-glucan (Nutrim (R)), even when used as the sole carbon. source. Similarly, strain BGRA43 grew satisfactorily in pure cow's or goat's milk as well as in the milk containing inulin or Nutrim (R). Using the probiotic strain BGRA43 as a single starter strain, fermented milk products obtained from cow's or goat's milk with or without inulin or Nutrim (R) contained about 10(7) CFU/mL. The products were homogeneous and viscous and the best sensory scores were observed for fermented milk beverage made from reconstituted skimmed milk, whole cow's milk and whole goat's milk supplemented with 1 % inulin.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Use of Lactobacillus helveticus BGRA43 for Manufacturing Fermented Milk Products
EP  - 265
IS  - 2
SP  - 257
VL  - 51
UR  - https://hdl.handle.net/21.15107/rcub_imagine_669
ER  - 

@article{
author = "Strahinić, Ivana and Lukić, Jovanka and Terzić-Vidojević, Amarela and Lozo, Jelena and Kojić, Milan and Topisirović, Ljubiša",
year = "2013",
abstract = "Lactobacillus helveticus BGRA43 isolated from human intestines shows antimicrobial activity against foodborne pathogens and during fermentation in milk releases peptides with demonstrated anti-inflammatory properties. In this study, it was found that strain BGRA43 exhibits antimicrobial activity against human pathogens Yersinia enterocolitica, Shigella sonnei, S. flexneri and Streptococcus pneumoniae. Strain BGRA43 was able to survive in simulated gastric juice containing milk and retained cell number stability during the incubation in simulated intestinal conditions. In addition, LC/MS/MS analysis showed the ability of BGRA43 to hydrolyze beta-lactoglobulin. Abundant growth of strain BGRA43 occurred in the presence of prebiotics inulin or concentrated oat bran beta-glucan (Nutrim (R)), even when used as the sole carbon. source. Similarly, strain BGRA43 grew satisfactorily in pure cow's or goat's milk as well as in the milk containing inulin or Nutrim (R). Using the probiotic strain BGRA43 as a single starter strain, fermented milk products obtained from cow's or goat's milk with or without inulin or Nutrim (R) contained about 10(7) CFU/mL. The products were homogeneous and viscous and the best sensory scores were observed for fermented milk beverage made from reconstituted skimmed milk, whole cow's milk and whole goat's milk supplemented with 1 % inulin.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Use of Lactobacillus helveticus BGRA43 for Manufacturing Fermented Milk Products",
pages = "265-257",
number = "2",
volume = "51",
url = "https://hdl.handle.net/21.15107/rcub_imagine_669"
}

Strahinić, I., Lukić, J., Terzić-Vidojević, A., Lozo, J., Kojić, M.,& Topisirović, L.. (2013). Use of Lactobacillus helveticus BGRA43 for Manufacturing Fermented Milk Products. in Food Technology and Biotechnology
University of Zagreb., 51(2), 257-265.
https://hdl.handle.net/21.15107/rcub_imagine_669

Strahinić I, Lukić J, Terzić-Vidojević A, Lozo J, Kojić M, Topisirović L. Use of Lactobacillus helveticus BGRA43 for Manufacturing Fermented Milk Products. in Food Technology and Biotechnology. 2013;51(2):257-265.
https://hdl.handle.net/21.15107/rcub_imagine_669 .

Strahinić, Ivana, Lukić, Jovanka, Terzić-Vidojević, Amarela, Lozo, Jelena, Kojić, Milan, Topisirović, Ljubiša, "Use of Lactobacillus helveticus BGRA43 for Manufacturing Fermented Milk Products" in Food Technology and Biotechnology, 51, no. 2 (2013):257-265,
https://hdl.handle.net/21.15107/rcub_imagine_669 .

TY  - JOUR
AU  - Kozoderović, Gordana
AU  - Velhner, Maja
AU  - Jelesić, Zora
AU  - Golić, Nataša
AU  - Lozo, Jelena
AU  - Kehrenberg, Corinna
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/583
AB  - The prevalence of quinolone resistance was studied in Salmonella enterica serotype Enteritidis isolates collected during 2005-2010 in Southern Ba. cka County, Serbia. A total of 878 clinical isolates were examined, among which 19 (2.2%) nalidixic acid (NAL)-resistant S. Enteritidis were detected by selection on agar plates containing 64 mg/L NAL. Antimicrobial susceptibility of the isolates was tested by the agar dilution method. According to Clinical and Laboratory Standards Institute (CLSI) breakpoints, ciprofloxacin (CIP) resistance was not present in the strains. Multiple drug resistance was rare, and resistance to NAL was most often present as a single resistance property. All but one NAL-resistant S. Enteritidis showed reduced susceptibility to CIP [i.e. minimum inhibitory concentration (MIC)  gt = 0.125 mg/L]. This isolate of human origin had a CIP MIC of 0.064 mg/L and DNA sequencing revealed that it contained an Asp87Gly gyrA mutation. Most of the remaining isolates had MICs for NAL and CIP of 256 mg/L and 0.256 mg/L, respectively. Mutations in the Asp87 codon resulted in substitutions to Asn in most of the isolates, but Asp87Gly and Ser83Phe exchanges were also detected. No mutations were present in the gyrB, parC or parE genes. Although CIP resistance was absent, reduced susceptibility characterised by mutations in gyrA was apparent among S. Enteritidis isolates from Serbia.
PB  - Elsevier, Amsterdam
T2  - International Journal of Antimicrobial Agents
T1  - Prevalence of quinolone resistance and mutations in the topoisomerase genes in Salmonella enterica serotype Enteritidis isolates from Serbia
EP  - 457
IS  - 5
SP  - 455
VL  - 40
DO  - 10.1016/j.ijantimicag.2012.07.012
ER  - 

@article{
author = "Kozoderović, Gordana and Velhner, Maja and Jelesić, Zora and Golić, Nataša and Lozo, Jelena and Kehrenberg, Corinna",
year = "2012",
abstract = "The prevalence of quinolone resistance was studied in Salmonella enterica serotype Enteritidis isolates collected during 2005-2010 in Southern Ba. cka County, Serbia. A total of 878 clinical isolates were examined, among which 19 (2.2%) nalidixic acid (NAL)-resistant S. Enteritidis were detected by selection on agar plates containing 64 mg/L NAL. Antimicrobial susceptibility of the isolates was tested by the agar dilution method. According to Clinical and Laboratory Standards Institute (CLSI) breakpoints, ciprofloxacin (CIP) resistance was not present in the strains. Multiple drug resistance was rare, and resistance to NAL was most often present as a single resistance property. All but one NAL-resistant S. Enteritidis showed reduced susceptibility to CIP [i.e. minimum inhibitory concentration (MIC)  gt = 0.125 mg/L]. This isolate of human origin had a CIP MIC of 0.064 mg/L and DNA sequencing revealed that it contained an Asp87Gly gyrA mutation. Most of the remaining isolates had MICs for NAL and CIP of 256 mg/L and 0.256 mg/L, respectively. Mutations in the Asp87 codon resulted in substitutions to Asn in most of the isolates, but Asp87Gly and Ser83Phe exchanges were also detected. No mutations were present in the gyrB, parC or parE genes. Although CIP resistance was absent, reduced susceptibility characterised by mutations in gyrA was apparent among S. Enteritidis isolates from Serbia.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Antimicrobial Agents",
title = "Prevalence of quinolone resistance and mutations in the topoisomerase genes in Salmonella enterica serotype Enteritidis isolates from Serbia",
pages = "457-455",
number = "5",
volume = "40",
doi = "10.1016/j.ijantimicag.2012.07.012"
}

Kozoderović, G., Velhner, M., Jelesić, Z., Golić, N., Lozo, J.,& Kehrenberg, C.. (2012). Prevalence of quinolone resistance and mutations in the topoisomerase genes in Salmonella enterica serotype Enteritidis isolates from Serbia. in International Journal of Antimicrobial Agents
Elsevier, Amsterdam., 40(5), 455-457.
https://doi.org/10.1016/j.ijantimicag.2012.07.012

Kozoderović G, Velhner M, Jelesić Z, Golić N, Lozo J, Kehrenberg C. Prevalence of quinolone resistance and mutations in the topoisomerase genes in Salmonella enterica serotype Enteritidis isolates from Serbia. in International Journal of Antimicrobial Agents. 2012;40(5):455-457.
doi:10.1016/j.ijantimicag.2012.07.012 .

Kozoderović, Gordana, Velhner, Maja, Jelesić, Zora, Golić, Nataša, Lozo, Jelena, Kehrenberg, Corinna, "Prevalence of quinolone resistance and mutations in the topoisomerase genes in Salmonella enterica serotype Enteritidis isolates from Serbia" in International Journal of Antimicrobial Agents, 40, no. 5 (2012):455-457,
https://doi.org/10.1016/j.ijantimicag.2012.07.012 . .

TY  - JOUR
AU  - Tolinački, Maja
AU  - Lozo, Jelena
AU  - Veljović, Katarina
AU  - Kojić, Milan
AU  - Fira, Đorđe
AU  - Topisirović, Ljubiša
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/556
AB  - Cilj ove studije je izučavanje antimikrobnog potencijala 52 prirodna izolata vrste L. casei/paracasei. Učestalost gena koji kodiraju BacSJ (bacSJ2-8/bacSJ2-8i genski klaster), acidocin 8912 (acdT), ABC-transporter (abcT) i pomoćni protein (acc) su takođe izučavani. Genski klaster bacSJ2-8/bacSJ2-8i prisutan je kod 49 (94.23%), a acdT kod 41 (78.85%) od 52 testirana soja. Četrdeset sojeva (76.92%) poseduje oba analizirana gena. Interesantno je da samo 17 sojeva (32.69%) koji poseduju bacSJ2-8/bacSJ2-8i genski klaster i/ili acdT gen proizvode bakteriocine. Soj L. paracasei BGNK1-62 poseduje bacSJ2-8/bacSJ2-8i genski klaster, ali ne proizvodi bakteriocin BacSJ što je verovatno posledica nedostatka abcT i acc gena. Nakon transformacije soja BGNK1-62 konstruktom pA2A koji poseduje abcT i acc gene ostvarena je proizvodnja bakteriocina BacSJ. Osim toga, utvrđeno je da soj L. paracasei BGGR2-66 proizvodi nov bakteriocin označen kao BacGR, koji je biohemijski okarakterisan, a određena je i njegova N-terminalna sekvenca.
AB  - The aim of this study was to investigate the antimicrobial potential of 52 natural isolates of Lactobacillus casei/paracasei. The incidence of relevant genes encoding BacSJ (bacSJ2-8/bacSJ2-8i gene cluster), acidocin 8912 (acdT), ABC-transporter (abcT) and accessory protein (acc) was also studied. These genes were found to be widespread amongst the analyzed L. casei/paracasei strains. The bacSJ2-8/bacSJ2-8i gene cluster was present in 49 (94.23%) and acdT in 41 (78.85%) of the 52 tested strains. Forty of these strains (76.92%) harbored both analyzed genes. Interestingly, only 17 strains (32.69%) with the bacSJ2-8/bacSJ2-8i gene cluster and/or the acdT gene showed bacteriocin production. Strain L. paracasei BGNK1-62 contained the bacSJ2-8/bacSJ2-8i gene cluster, but did not produce bacteriocin BacSJ possibly due to absence of the abcT and acc genes. Hence, these genes were introduced into BGNK1-62 by transformation with constructed plasmid pA2A, after which BacSJ was produced. In addition, it was found that L. paracasei BGGR2-66 produced new bacteriocin designated as BacGR that was biochemically characterized and its N- terminal sequence was determined.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Izučavanje antimikrobnog potencijala prirodnih izolata Lactobacillus casei/paracasei grupe
T1  - Examination of antimicrobial potential in natural isolates of lactobacillus casei/paracasei group
EP  - 677
IS  - 3
SP  - 661
VL  - 44
DO  - 10.2298/GENSR1203661T
ER  - 

@article{
author = "Tolinački, Maja and Lozo, Jelena and Veljović, Katarina and Kojić, Milan and Fira, Đorđe and Topisirović, Ljubiša",
year = "2012",
abstract = "Cilj ove studije je izučavanje antimikrobnog potencijala 52 prirodna izolata vrste L. casei/paracasei. Učestalost gena koji kodiraju BacSJ (bacSJ2-8/bacSJ2-8i genski klaster), acidocin 8912 (acdT), ABC-transporter (abcT) i pomoćni protein (acc) su takođe izučavani. Genski klaster bacSJ2-8/bacSJ2-8i prisutan je kod 49 (94.23%), a acdT kod 41 (78.85%) od 52 testirana soja. Četrdeset sojeva (76.92%) poseduje oba analizirana gena. Interesantno je da samo 17 sojeva (32.69%) koji poseduju bacSJ2-8/bacSJ2-8i genski klaster i/ili acdT gen proizvode bakteriocine. Soj L. paracasei BGNK1-62 poseduje bacSJ2-8/bacSJ2-8i genski klaster, ali ne proizvodi bakteriocin BacSJ što je verovatno posledica nedostatka abcT i acc gena. Nakon transformacije soja BGNK1-62 konstruktom pA2A koji poseduje abcT i acc gene ostvarena je proizvodnja bakteriocina BacSJ. Osim toga, utvrđeno je da soj L. paracasei BGGR2-66 proizvodi nov bakteriocin označen kao BacGR, koji je biohemijski okarakterisan, a određena je i njegova N-terminalna sekvenca., The aim of this study was to investigate the antimicrobial potential of 52 natural isolates of Lactobacillus casei/paracasei. The incidence of relevant genes encoding BacSJ (bacSJ2-8/bacSJ2-8i gene cluster), acidocin 8912 (acdT), ABC-transporter (abcT) and accessory protein (acc) was also studied. These genes were found to be widespread amongst the analyzed L. casei/paracasei strains. The bacSJ2-8/bacSJ2-8i gene cluster was present in 49 (94.23%) and acdT in 41 (78.85%) of the 52 tested strains. Forty of these strains (76.92%) harbored both analyzed genes. Interestingly, only 17 strains (32.69%) with the bacSJ2-8/bacSJ2-8i gene cluster and/or the acdT gene showed bacteriocin production. Strain L. paracasei BGNK1-62 contained the bacSJ2-8/bacSJ2-8i gene cluster, but did not produce bacteriocin BacSJ possibly due to absence of the abcT and acc genes. Hence, these genes were introduced into BGNK1-62 by transformation with constructed plasmid pA2A, after which BacSJ was produced. In addition, it was found that L. paracasei BGGR2-66 produced new bacteriocin designated as BacGR that was biochemically characterized and its N- terminal sequence was determined.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Izučavanje antimikrobnog potencijala prirodnih izolata Lactobacillus casei/paracasei grupe, Examination of antimicrobial potential in natural isolates of lactobacillus casei/paracasei group",
pages = "677-661",
number = "3",
volume = "44",
doi = "10.2298/GENSR1203661T"
}

Tolinački, M., Lozo, J., Veljović, K., Kojić, M., Fira, Đ.,& Topisirović, L.. (2012). Izučavanje antimikrobnog potencijala prirodnih izolata Lactobacillus casei/paracasei grupe. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 44(3), 661-677.
https://doi.org/10.2298/GENSR1203661T

Tolinački M, Lozo J, Veljović K, Kojić M, Fira Đ, Topisirović L. Izučavanje antimikrobnog potencijala prirodnih izolata Lactobacillus casei/paracasei grupe. in Genetika-Belgrade. 2012;44(3):661-677.
doi:10.2298/GENSR1203661T .

Tolinački, Maja, Lozo, Jelena, Veljović, Katarina, Kojić, Milan, Fira, Đorđe, Topisirović, Ljubiša, "Izučavanje antimikrobnog potencijala prirodnih izolata Lactobacillus casei/paracasei grupe" in Genetika-Belgrade, 44, no. 3 (2012):661-677,
https://doi.org/10.2298/GENSR1203661T . .

TY  - JOUR
AU  - Lozo, Jelena
AU  - Strahinić, Ivana
AU  - Dalgalarrondo, Michele
AU  - Chobert, Jean-Marc
AU  - Haertle, Thomas
AU  - Topisirović, Ljubiša
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/515
AB  - Lactic acid bacteria contain different cell-envelope proteinases responsible for hydrolysis of caseins. Isolates of Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 harbour PrtP and PrtR proteinases, respectively, whereas the PrtH proteinase of Lactobacillus helveticus BGRA43 is similar to that of Lb. helveticus CNRZ32. Mass spectrometry analysis of the major peptides isolated by reversed phase-high performance liquid chromatography allowed the identification of 25, 22 and 17 peptides after beta-casein hydrolysis by strains BGHN14, BGRA43 and BGT10, respectively. Regardless of the type of proteinase, beta-casein was hydrolyzed preferentially after hydrophobic residues and glutamines ( Q). PrtP and PrtR proteinases preferentially targeted the C-terminus of beta-casein. In the case of PrtH proteinase, most of the peptides obtained were cleaved from N-terminus of this casein. Nine identical peptides were identified after hydrolysis with PrtP and PrtR, three after proteolysis with PrtR and PrtH, and two after hydrolysis with all three proteinases studied.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Dairy Journal
T1  - Comparative analysis of beta-casein proteolysis by PrtP proteinase from Lactobacillus paracasei subsp paracasei BGHN14, PrtR proteinase from Lactobacillus rhamnosus BGT10 and PrtH proteinase from Lactobacillus helveticus BGRA43
EP  - 868
IS  - 11
SP  - 863
VL  - 21
DO  - 10.1016/j.idairyj.2011.05.002
ER  - 

@article{
author = "Lozo, Jelena and Strahinić, Ivana and Dalgalarrondo, Michele and Chobert, Jean-Marc and Haertle, Thomas and Topisirović, Ljubiša",
year = "2011",
abstract = "Lactic acid bacteria contain different cell-envelope proteinases responsible for hydrolysis of caseins. Isolates of Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 harbour PrtP and PrtR proteinases, respectively, whereas the PrtH proteinase of Lactobacillus helveticus BGRA43 is similar to that of Lb. helveticus CNRZ32. Mass spectrometry analysis of the major peptides isolated by reversed phase-high performance liquid chromatography allowed the identification of 25, 22 and 17 peptides after beta-casein hydrolysis by strains BGHN14, BGRA43 and BGT10, respectively. Regardless of the type of proteinase, beta-casein was hydrolyzed preferentially after hydrophobic residues and glutamines ( Q). PrtP and PrtR proteinases preferentially targeted the C-terminus of beta-casein. In the case of PrtH proteinase, most of the peptides obtained were cleaved from N-terminus of this casein. Nine identical peptides were identified after hydrolysis with PrtP and PrtR, three after proteolysis with PrtR and PrtH, and two after hydrolysis with all three proteinases studied.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Dairy Journal",
title = "Comparative analysis of beta-casein proteolysis by PrtP proteinase from Lactobacillus paracasei subsp paracasei BGHN14, PrtR proteinase from Lactobacillus rhamnosus BGT10 and PrtH proteinase from Lactobacillus helveticus BGRA43",
pages = "868-863",
number = "11",
volume = "21",
doi = "10.1016/j.idairyj.2011.05.002"
}

Lozo, J., Strahinić, I., Dalgalarrondo, M., Chobert, J., Haertle, T.,& Topisirović, L.. (2011). Comparative analysis of beta-casein proteolysis by PrtP proteinase from Lactobacillus paracasei subsp paracasei BGHN14, PrtR proteinase from Lactobacillus rhamnosus BGT10 and PrtH proteinase from Lactobacillus helveticus BGRA43. in International Dairy Journal
Elsevier Sci Ltd, Oxford., 21(11), 863-868.
https://doi.org/10.1016/j.idairyj.2011.05.002

Lozo J, Strahinić I, Dalgalarrondo M, Chobert J, Haertle T, Topisirović L. Comparative analysis of beta-casein proteolysis by PrtP proteinase from Lactobacillus paracasei subsp paracasei BGHN14, PrtR proteinase from Lactobacillus rhamnosus BGT10 and PrtH proteinase from Lactobacillus helveticus BGRA43. in International Dairy Journal. 2011;21(11):863-868.
doi:10.1016/j.idairyj.2011.05.002 .

Lozo, Jelena, Strahinić, Ivana, Dalgalarrondo, Michele, Chobert, Jean-Marc, Haertle, Thomas, Topisirović, Ljubiša, "Comparative analysis of beta-casein proteolysis by PrtP proteinase from Lactobacillus paracasei subsp paracasei BGHN14, PrtR proteinase from Lactobacillus rhamnosus BGT10 and PrtH proteinase from Lactobacillus helveticus BGRA43" in International Dairy Journal, 21, no. 11 (2011):863-868,
https://doi.org/10.1016/j.idairyj.2011.05.002 . .

TY  - JOUR
AU  - Kojić, Milan
AU  - Jovčić, Branko
AU  - Strahinić, Ivana
AU  - Begović, Jelena
AU  - Lozo, Jelena
AU  - Veljović, Katarina
AU  - Topisirović, Ljubiša
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/501
AB  - Background: Aggregation may play a main role in the adhesion of bacteria to the gastrointestinal epithelium and their colonization ability, as well as in probiotic effects through co-aggregation with intestinal pathogens and their subsequent removal. The aggregation phenomenon in lactococci is directly associated with the sex factor and lactose plasmid co-integration event or duplication of the cell wall spanning (CWS) domain of PrtP proteinase. Results: Lactococcus lactis subsp. lactis BGKP1 was isolated from artisanal semi-hard homemade cheese and selected due to its strong auto-aggregation phenotype. Subsequently, non-aggregating derivative (Agg(-)) of BGKP1, designated as BGKP1-20, was isolated, too. Comparative analysis of cell surface proteins of BGKP1 and derivative BGKP1-20 revealed a protein of approximately 200 kDa only in the parental strain BGKP1. The gene involved in aggregation (aggL) was mapped on plasmid pKP1 (16.2 kb), cloned and expressed in homologous and heterologous lactococci and enterococci. This novel lactococcal aggregation protein was shown to be sufficient for cell aggregation in all tested hosts. In addition to the aggL gene, six more ORFs involved in replication (repB and repX), restriction and modification (hsdS), transposition (tnp) and possible interaction with mucin (mbpL) were also located on plasmid pKP1. Conclusion: AggL is a new protein belonging to the collagen-binding superfamily of proteins and is sufficient for cell aggregation in lactococci.
PB  - Biomed Central Ltd, London
T2  - BMC Microbiology
T1  - Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp lactis BGKP1
VL  - 11
DO  - 10.1186/1471-2180-11-265
ER  - 

@article{
author = "Kojić, Milan and Jovčić, Branko and Strahinić, Ivana and Begović, Jelena and Lozo, Jelena and Veljović, Katarina and Topisirović, Ljubiša",
year = "2011",
abstract = "Background: Aggregation may play a main role in the adhesion of bacteria to the gastrointestinal epithelium and their colonization ability, as well as in probiotic effects through co-aggregation with intestinal pathogens and their subsequent removal. The aggregation phenomenon in lactococci is directly associated with the sex factor and lactose plasmid co-integration event or duplication of the cell wall spanning (CWS) domain of PrtP proteinase. Results: Lactococcus lactis subsp. lactis BGKP1 was isolated from artisanal semi-hard homemade cheese and selected due to its strong auto-aggregation phenotype. Subsequently, non-aggregating derivative (Agg(-)) of BGKP1, designated as BGKP1-20, was isolated, too. Comparative analysis of cell surface proteins of BGKP1 and derivative BGKP1-20 revealed a protein of approximately 200 kDa only in the parental strain BGKP1. The gene involved in aggregation (aggL) was mapped on plasmid pKP1 (16.2 kb), cloned and expressed in homologous and heterologous lactococci and enterococci. This novel lactococcal aggregation protein was shown to be sufficient for cell aggregation in all tested hosts. In addition to the aggL gene, six more ORFs involved in replication (repB and repX), restriction and modification (hsdS), transposition (tnp) and possible interaction with mucin (mbpL) were also located on plasmid pKP1. Conclusion: AggL is a new protein belonging to the collagen-binding superfamily of proteins and is sufficient for cell aggregation in lactococci.",
publisher = "Biomed Central Ltd, London",
journal = "BMC Microbiology",
title = "Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp lactis BGKP1",
volume = "11",
doi = "10.1186/1471-2180-11-265"
}

Kojić, M., Jovčić, B., Strahinić, I., Begović, J., Lozo, J., Veljović, K.,& Topisirović, L.. (2011). Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp lactis BGKP1. in BMC Microbiology
Biomed Central Ltd, London., 11.
https://doi.org/10.1186/1471-2180-11-265

Kojić M, Jovčić B, Strahinić I, Begović J, Lozo J, Veljović K, Topisirović L. Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp lactis BGKP1. in BMC Microbiology. 2011;11.
doi:10.1186/1471-2180-11-265 .

Kojić, Milan, Jovčić, Branko, Strahinić, Ivana, Begović, Jelena, Lozo, Jelena, Veljović, Katarina, Topisirović, Ljubiša, "Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp lactis BGKP1" in BMC Microbiology, 11 (2011),
https://doi.org/10.1186/1471-2180-11-265 . .

TY  - JOUR
AU  - Tolinački, Maja
AU  - Kojić, Milan
AU  - Lozo, Jelena
AU  - Terzić-Vidojević, Amarela
AU  - Topisirović, Ljubiša
AU  - Fira, Đorđe
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/405
AB  - The strain Lactobacillus paracasei subsp. paracasei BGUB9 that was isolated from traditionally homemade hard cheese produces bacteriocin designated as BacUB9, with an approximate molecular mass of 4 kDa. Biochemical characterization and the antimicrobial activity test of BacUB9 were performed. The onset of BacUB9 biosynthesis was observed at the end of an exponential phase of growth. Bacteriocin UB9 retained the antimicrobial activity within the pH range from 1 to 10 and after treatment at 100oC for 30 min. The bacteriocin is susceptible to the activity of proteolytic enzymes. Bacteriocin BacUB9 has a very narrow antimicrobial spectrum, limited to several strains that belong to closely related species. The effect of BGUB9 on the growth of the strain Lactobacillus paracasei subsp. paracasei BGHN14 in a mixed culture was monitored. The mode of action of BacUB9 on the strain BGHN14 was identified as bacteriostatic. Plasmid curing results indicated that a plasmid, designated as pUB9, seemed to be responsible for both bacteriocin BacUB9 production and host immunity.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9
EP  - 899
IS  - 4
SP  - 889
VL  - 62
DO  - 10.2298/ABS1004889T
ER  - 

@article{
author = "Tolinački, Maja and Kojić, Milan and Lozo, Jelena and Terzić-Vidojević, Amarela and Topisirović, Ljubiša and Fira, Đorđe",
year = "2010",
abstract = "The strain Lactobacillus paracasei subsp. paracasei BGUB9 that was isolated from traditionally homemade hard cheese produces bacteriocin designated as BacUB9, with an approximate molecular mass of 4 kDa. Biochemical characterization and the antimicrobial activity test of BacUB9 were performed. The onset of BacUB9 biosynthesis was observed at the end of an exponential phase of growth. Bacteriocin UB9 retained the antimicrobial activity within the pH range from 1 to 10 and after treatment at 100oC for 30 min. The bacteriocin is susceptible to the activity of proteolytic enzymes. Bacteriocin BacUB9 has a very narrow antimicrobial spectrum, limited to several strains that belong to closely related species. The effect of BGUB9 on the growth of the strain Lactobacillus paracasei subsp. paracasei BGHN14 in a mixed culture was monitored. The mode of action of BacUB9 on the strain BGHN14 was identified as bacteriostatic. Plasmid curing results indicated that a plasmid, designated as pUB9, seemed to be responsible for both bacteriocin BacUB9 production and host immunity.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9",
pages = "899-889",
number = "4",
volume = "62",
doi = "10.2298/ABS1004889T"
}

Tolinački, M., Kojić, M., Lozo, J., Terzić-Vidojević, A., Topisirović, L.,& Fira, Đ.. (2010). Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(4), 889-899.
https://doi.org/10.2298/ABS1004889T

Tolinački M, Kojić M, Lozo J, Terzić-Vidojević A, Topisirović L, Fira Đ. Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9. in Archives of Biological Sciences. 2010;62(4):889-899.
doi:10.2298/ABS1004889T .

Tolinački, Maja, Kojić, Milan, Lozo, Jelena, Terzić-Vidojević, Amarela, Topisirović, Ljubiša, Fira, Đorđe, "Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. paracasei BGUB9" in Archives of Biological Sciences, 62, no. 4 (2010):889-899,
https://doi.org/10.2298/ABS1004889T . .