TY  - CONF
AU  - Plačkić, Nikola
AU  - Kljajević, Nemanja
AU  - Obradović, Mina
AU  - Kekić, Dušan
AU  - Gajić, Ina
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2383
AB  - Anti-microbial drug resistance (AMR) is one of
the global health threats caused by the misuse
of drugs typically used to treat microbial
infections in humans, animals and plants. AMR
in nosocomial infections not only significantly
hinders treatment and endangers the patients’
lives, but also elevates the costs of healthcare.
Multiple research approaches have been initiated
to combat AMR, and one promising method
is bacteriophage therapy. Bacteriophages (phages)
are viruses that naturally exploit bacteria as
their hosts for replication and can cause cell lysis,
which makes them promising candidates for
treating the infections that do not respond to
conventional antibiotic therapies. In this study,
we screened wastewater samples from four
different collectors in Belgrade urban area for
bacteriophages active against clinically isolated
strains of two biofilm-producing bacteria that
readily persist in hospital environment - Klebsiella
pneumoniae (6 strains) and Pseudomonas aeruginosa
(2 strains). Wastewaters were screened
for phage presence through phage enrichment
process, in which bacteria were grown in a mixture
of water samples and nutrient-rich broth.
Obtained cultures were screened for antimicrobial
activity against the respective host strains,
and candidates were subjected to a first-round
plaque assay to detect the phages. Finally, the
activity of all the candidates was tested against
all strains of the same species to gain the first insight
into their host range. We discovered 20 potentially
distinct bacteriophages active against
K. pneumoniae strains and two potentially different
candidates targeting P. aeruginosa. Notably,
one phage exhibited activity against all tested K.
pneumoniae strains, and four were active against
5 out of 6 tested strains. Among 22 candidates in
total, five showed depolymerizing activity, indicating
promise in combating biofilm formation.
Currently, isolation of new phages, as well as purification
and host range analysis is underway for
several candidates targeting K. pneumoniae and
two targeting P. aeruginosa strains.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - NOVEL BACTERIOPHAGE ISOLATION FROM BELGRADE WASTEWATERS
EP  - 148
SP  - 148
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2383
ER  - 

@conference{
author = "Plačkić, Nikola and Kljajević, Nemanja and Obradović, Mina and Kekić, Dušan and Gajić, Ina and Stanisavljević, Nemanja and Vukotić, Goran",
year = "2024",
abstract = "Anti-microbial drug resistance (AMR) is one of
the global health threats caused by the misuse
of drugs typically used to treat microbial
infections in humans, animals and plants. AMR
in nosocomial infections not only significantly
hinders treatment and endangers the patients’
lives, but also elevates the costs of healthcare.
Multiple research approaches have been initiated
to combat AMR, and one promising method
is bacteriophage therapy. Bacteriophages (phages)
are viruses that naturally exploit bacteria as
their hosts for replication and can cause cell lysis,
which makes them promising candidates for
treating the infections that do not respond to
conventional antibiotic therapies. In this study,
we screened wastewater samples from four
different collectors in Belgrade urban area for
bacteriophages active against clinically isolated
strains of two biofilm-producing bacteria that
readily persist in hospital environment - Klebsiella
pneumoniae (6 strains) and Pseudomonas aeruginosa
(2 strains). Wastewaters were screened
for phage presence through phage enrichment
process, in which bacteria were grown in a mixture
of water samples and nutrient-rich broth.
Obtained cultures were screened for antimicrobial
activity against the respective host strains,
and candidates were subjected to a first-round
plaque assay to detect the phages. Finally, the
activity of all the candidates was tested against
all strains of the same species to gain the first insight
into their host range. We discovered 20 potentially
distinct bacteriophages active against
K. pneumoniae strains and two potentially different
candidates targeting P. aeruginosa. Notably,
one phage exhibited activity against all tested K.
pneumoniae strains, and four were active against
5 out of 6 tested strains. Among 22 candidates in
total, five showed depolymerizing activity, indicating
promise in combating biofilm formation.
Currently, isolation of new phages, as well as purification
and host range analysis is underway for
several candidates targeting K. pneumoniae and
two targeting P. aeruginosa strains.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "NOVEL BACTERIOPHAGE ISOLATION FROM BELGRADE WASTEWATERS",
pages = "148-148",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2383"
}

Plačkić, N., Kljajević, N., Obradović, M., Kekić, D., Gajić, I., Stanisavljević, N.,& Vukotić, G.. (2024). NOVEL BACTERIOPHAGE ISOLATION FROM BELGRADE WASTEWATERS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 148-148.
https://hdl.handle.net/21.15107/rcub_imagine_2383

Plačkić N, Kljajević N, Obradović M, Kekić D, Gajić I, Stanisavljević N, Vukotić G. NOVEL BACTERIOPHAGE ISOLATION FROM BELGRADE WASTEWATERS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:148-148.
https://hdl.handle.net/21.15107/rcub_imagine_2383 .

Plačkić, Nikola, Kljajević, Nemanja, Obradović, Mina, Kekić, Dušan, Gajić, Ina, Stanisavljević, Nemanja, Vukotić, Goran, "NOVEL BACTERIOPHAGE ISOLATION FROM BELGRADE WASTEWATERS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):148-148,
https://hdl.handle.net/21.15107/rcub_imagine_2383 .

TY  - JOUR
AU  - Miloradović, Zorana
AU  - Kovačević, Jovana
AU  - Miočionović, Jelena
AU  - Đekić, Ilija
AU  - Kljajević, Nemanja
AU  - Smigić, Nada
PY  - 2024
UR  - https://www.cell.com/heliyon/abstract/S2405-8440(24)03473-X
UR  - http://www.ncbi.nlm.nih.gov/pubmed/38187278
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2343
AB  - The objective of this study was to identify the requirements needed for selling dairy products through e-commerce, as well as current gaps and challenges that exist for small scale dairy processors (SSDPs), and need to be addressed in order to comply with those requirements. A mixed method research design was used for training needs assessment. Qualitative (in-depth interview with 7 online platform representatives (OPRs)) and quantitative approach (survey questionnaire with 58 SSDPs) were conducted. Interview transcripts were coded and codes were grouped into seven themes. Hierarchical cluster analysis was applied to 146 answers from 58 SSDPs. They were divided into 4 clusters. Mean sums of responses between clusters were compared by Mann-Whitney U test. OPRs suggested that SSDPs should be provided with tools and resources to help them achieve food safety and quality targets, as well as practical knowledge and skills. They reported that it is crucial to find a solution for the cold chain transportation, for maintaining consistent product quality. Survey results showed that SSDPs use kitchen equipment (79.3%) and kitchen cleaning products (81.0%) for dairy processing. In total, 43.1% process raw milk and only 24.1% have product label on the package. Only members of cluster 3 and 4 sell their products online (73.7% and 90.0%, respectively), mostly using their own social media platforms (57.9% and 60.0%, respectively), transporting products to end buyers by themselves in hand refrigerators (47.4% and 70.0%, respectively). By analyzing the differences among clusters of SSDPs, trainings can be tailored to the characteristics and knowledge gaps of each group.
PB  - CellPress
T2  - Heliyon
T2  - HeliyonHeliyon
T1  - E-commerce readiness and training needs of small-scale dairy processors in Serbia: Understanding barriers and knowledge gaps
IS  - 6
VL  - 10
DO  - 10.1016/j.heliyon.2024.e27442
ER  - 

@article{
author = "Miloradović, Zorana and Kovačević, Jovana and Miočionović, Jelena and Đekić, Ilija and Kljajević, Nemanja and Smigić, Nada",
year = "2024",
abstract = "The objective of this study was to identify the requirements needed for selling dairy products through e-commerce, as well as current gaps and challenges that exist for small scale dairy processors (SSDPs), and need to be addressed in order to comply with those requirements. A mixed method research design was used for training needs assessment. Qualitative (in-depth interview with 7 online platform representatives (OPRs)) and quantitative approach (survey questionnaire with 58 SSDPs) were conducted. Interview transcripts were coded and codes were grouped into seven themes. Hierarchical cluster analysis was applied to 146 answers from 58 SSDPs. They were divided into 4 clusters. Mean sums of responses between clusters were compared by Mann-Whitney U test. OPRs suggested that SSDPs should be provided with tools and resources to help them achieve food safety and quality targets, as well as practical knowledge and skills. They reported that it is crucial to find a solution for the cold chain transportation, for maintaining consistent product quality. Survey results showed that SSDPs use kitchen equipment (79.3%) and kitchen cleaning products (81.0%) for dairy processing. In total, 43.1% process raw milk and only 24.1% have product label on the package. Only members of cluster 3 and 4 sell their products online (73.7% and 90.0%, respectively), mostly using their own social media platforms (57.9% and 60.0%, respectively), transporting products to end buyers by themselves in hand refrigerators (47.4% and 70.0%, respectively). By analyzing the differences among clusters of SSDPs, trainings can be tailored to the characteristics and knowledge gaps of each group.",
publisher = "CellPress",
journal = "Heliyon, HeliyonHeliyon",
title = "E-commerce readiness and training needs of small-scale dairy processors in Serbia: Understanding barriers and knowledge gaps",
number = "6",
volume = "10",
doi = "10.1016/j.heliyon.2024.e27442"
}

Miloradović, Z., Kovačević, J., Miočionović, J., Đekić, I., Kljajević, N.,& Smigić, N.. (2024). E-commerce readiness and training needs of small-scale dairy processors in Serbia: Understanding barriers and knowledge gaps. in Heliyon
CellPress., 10(6).
https://doi.org/10.1016/j.heliyon.2024.e27442

Miloradović Z, Kovačević J, Miočionović J, Đekić I, Kljajević N, Smigić N. E-commerce readiness and training needs of small-scale dairy processors in Serbia: Understanding barriers and knowledge gaps. in Heliyon. 2024;10(6).
doi:10.1016/j.heliyon.2024.e27442 .

Miloradović, Zorana, Kovačević, Jovana, Miočionović, Jelena, Đekić, Ilija, Kljajević, Nemanja, Smigić, Nada, "E-commerce readiness and training needs of small-scale dairy processors in Serbia: Understanding barriers and knowledge gaps" in Heliyon, 10, no. 6 (2024),
https://doi.org/10.1016/j.heliyon.2024.e27442 . .

TY  - CONF
AU  - Vukotić, Goran
AU  - Obradović, Mina
AU  - Plačkić, Nikola
AU  - Kljajević, Nemanja
AU  - Pavić, Aleksandar
AU  - Kekić, Dušan
AU  - Gajić, Ina
AU  - Kojić, Milan
AU  - Stanisavljević, Nemanja
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2375
AB  - Antimicrobial resistance (AMR) arises when
bacteria and other microbes stop responding
to medications. AMR is now recognized as one
of serious global health threats, repeatedly
appearing in the World Health Organization’s
(WHO) lists of urgent global health challenges,
including the 2024 list. It is taking a fatal toll
– nearly 5 million deaths globally per year are
associated with AMR, encompassing 1.27 million
directly attributed to AMR. The COVID-19
pandemic paved the way for aggravation of
bacterial AMR – primarily due to enhancement
in unspecific and unjustified prescription and
use of broad-spectrum antibiotics, resulting in
what is now recognized as „silent pandemic of
AMR“. Bacteriophages (phages) are natural and
specific predators of bacteria - viruses that can
infect, replicate inside and lyse arguably any
bacteria. Their therapeutic potential is being
hastily evaluated through different approaches:
in silico, in vitro, ex vivo and in vivo – in laboratory
animals as well as in human case and clinical
studies. Although the results are promising,bacteria rapidly develop resistance against
phages, which why the isolation and research
of new phages is needed. Our work is concentrated
on three bacterial species for which critical
priority by WHO has been declared – carbapenem-
resistant Acinetobacter baumannii,
Pseudomonas aeruginosa and Klebsiella pneumoniae.
Twenty distinct pathogenic strains of
A. baumannii, 6 K. pneumoniae and 6 P. aeruginosa
were used as targets for bacteriophage
isolation, and total of 14, 22 and 8 potentially
distinct phages were collected, respectively. All
strains were nosocomial isolates obtained from
various tissues, including from terminally ill patients.
Six phages were characterized in detail.
In particular, phage vB_AbaM_ISTD was applied
against A. baumannii in zebrafish embryo
model of systemic infection, and demonstrated
powerful therapeutic potential, eradicating the
infection. Interestingly, its DNA was characterized
with highly modified thymidine (amassing
1228 Da), making it the largest non-canonical
deoxynucleoside reported so far.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE
EP  - 121
SP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2375
ER  - 

@conference{
author = "Vukotić, Goran and Obradović, Mina and Plačkić, Nikola and Kljajević, Nemanja and Pavić, Aleksandar and Kekić, Dušan and Gajić, Ina and Kojić, Milan and Stanisavljević, Nemanja",
year = "2024",
abstract = "Antimicrobial resistance (AMR) arises when
bacteria and other microbes stop responding
to medications. AMR is now recognized as one
of serious global health threats, repeatedly
appearing in the World Health Organization’s
(WHO) lists of urgent global health challenges,
including the 2024 list. It is taking a fatal toll
– nearly 5 million deaths globally per year are
associated with AMR, encompassing 1.27 million
directly attributed to AMR. The COVID-19
pandemic paved the way for aggravation of
bacterial AMR – primarily due to enhancement
in unspecific and unjustified prescription and
use of broad-spectrum antibiotics, resulting in
what is now recognized as „silent pandemic of
AMR“. Bacteriophages (phages) are natural and
specific predators of bacteria - viruses that can
infect, replicate inside and lyse arguably any
bacteria. Their therapeutic potential is being
hastily evaluated through different approaches:
in silico, in vitro, ex vivo and in vivo – in laboratory
animals as well as in human case and clinical
studies. Although the results are promising,bacteria rapidly develop resistance against
phages, which why the isolation and research
of new phages is needed. Our work is concentrated
on three bacterial species for which critical
priority by WHO has been declared – carbapenem-
resistant Acinetobacter baumannii,
Pseudomonas aeruginosa and Klebsiella pneumoniae.
Twenty distinct pathogenic strains of
A. baumannii, 6 K. pneumoniae and 6 P. aeruginosa
were used as targets for bacteriophage
isolation, and total of 14, 22 and 8 potentially
distinct phages were collected, respectively. All
strains were nosocomial isolates obtained from
various tissues, including from terminally ill patients.
Six phages were characterized in detail.
In particular, phage vB_AbaM_ISTD was applied
against A. baumannii in zebrafish embryo
model of systemic infection, and demonstrated
powerful therapeutic potential, eradicating the
infection. Interestingly, its DNA was characterized
with highly modified thymidine (amassing
1228 Da), making it the largest non-canonical
deoxynucleoside reported so far.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE",
pages = "121-121",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2375"
}

Vukotić, G., Obradović, M., Plačkić, N., Kljajević, N., Pavić, A., Kekić, D., Gajić, I., Kojić, M.,& Stanisavljević, N.. (2024). BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 121-121.
https://hdl.handle.net/21.15107/rcub_imagine_2375

Vukotić G, Obradović M, Plačkić N, Kljajević N, Pavić A, Kekić D, Gajić I, Kojić M, Stanisavljević N. BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:121-121.
https://hdl.handle.net/21.15107/rcub_imagine_2375 .

Vukotić, Goran, Obradović, Mina, Plačkić, Nikola, Kljajević, Nemanja, Pavić, Aleksandar, Kekić, Dušan, Gajić, Ina, Kojić, Milan, Stanisavljević, Nemanja, "BACTERIOPHAGES OF MULTIDRUG-RESISTANT NOSOCOMIAL PATHOGENS – BELGRADE EXPERIENCE" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):121-121,
https://hdl.handle.net/21.15107/rcub_imagine_2375 .

TY  - CONF
AU  - Matijašević, Danka
AU  - Pavlović, Vladimir
AU  - Kljajević, Nemanja
AU  - Gardijan, Lazar
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2367
AB  - In last decades, bacterial resistance to first choice
antibiotics has been drastically increasing, therefore,
the research of new antimicrobial substances
is of great importance. This rising problem
with bacterial resistance to existing antibiotics
affects not only the health care institutes but also
food plants. S. aureus and S. enteritidis pose a
number of challenges to the food industry and
cause foodborne illness in humans. In addition,
due to their favourable elemental composition,
oyster mushrooms (Pleurotus sp.) are a reservoir
of bioactive compounds that give them remarkable
antibacterial potential. P. ostreatus is of
great economic importance and is the second
most cultivated edible mushroom, therefore information
about its possible targets on bacterial
cells is of great importance for use as a dietary
supplement or medicinal purposes. The results of
the antibacterial assay showed that tested bacterial
strains were susceptible to the methanol extract of P. ostreatus (PoME), while microbicidal
activity was only detected against Gram-positive
bacteria. Scanning electron microscopy (SEM)
micrographs suggested that extract acted on
cytoplasmic membrane of S. aureus, while the
cell envelope of S. Enteritidis was the most likely
target. Natural extracts may outperform individual
bioactive compounds due to the synergistic
interaction between the metabolites, which can
enhance the effects of the individual components.
Extracts rich in antibacterials are emerging
as alternatives to synthetic antibiotics in the food
and health sectors. Among these, crude mushroom
extracts are particularly sought after for
their diverse bioactive ingredients, as they can
combat resistant strains of bacteria due to their
different targets and modes of action. PoME can
be used as an effective antimicrobial agent, suitable
for applications aiming to eradicate foodborne
pathogens, thus enhancing food safety.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - MORPHOLOGICAL CHANGES OF STAPHYLOCOCCUS AUREUS AND SALMONELLA ENTERITIDIS UPON EXPOSURE TO THE EXTRACT OBTAINED FROM PLEUROTUS OSTREATUS MUSHROOM
EP  - 37
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2367
ER  - 

@conference{
author = "Matijašević, Danka and Pavlović, Vladimir and Kljajević, Nemanja and Gardijan, Lazar",
year = "2024",
abstract = "In last decades, bacterial resistance to first choice
antibiotics has been drastically increasing, therefore,
the research of new antimicrobial substances
is of great importance. This rising problem
with bacterial resistance to existing antibiotics
affects not only the health care institutes but also
food plants. S. aureus and S. enteritidis pose a
number of challenges to the food industry and
cause foodborne illness in humans. In addition,
due to their favourable elemental composition,
oyster mushrooms (Pleurotus sp.) are a reservoir
of bioactive compounds that give them remarkable
antibacterial potential. P. ostreatus is of
great economic importance and is the second
most cultivated edible mushroom, therefore information
about its possible targets on bacterial
cells is of great importance for use as a dietary
supplement or medicinal purposes. The results of
the antibacterial assay showed that tested bacterial
strains were susceptible to the methanol extract of P. ostreatus (PoME), while microbicidal
activity was only detected against Gram-positive
bacteria. Scanning electron microscopy (SEM)
micrographs suggested that extract acted on
cytoplasmic membrane of S. aureus, while the
cell envelope of S. Enteritidis was the most likely
target. Natural extracts may outperform individual
bioactive compounds due to the synergistic
interaction between the metabolites, which can
enhance the effects of the individual components.
Extracts rich in antibacterials are emerging
as alternatives to synthetic antibiotics in the food
and health sectors. Among these, crude mushroom
extracts are particularly sought after for
their diverse bioactive ingredients, as they can
combat resistant strains of bacteria due to their
different targets and modes of action. PoME can
be used as an effective antimicrobial agent, suitable
for applications aiming to eradicate foodborne
pathogens, thus enhancing food safety.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "MORPHOLOGICAL CHANGES OF STAPHYLOCOCCUS AUREUS AND SALMONELLA ENTERITIDIS UPON EXPOSURE TO THE EXTRACT OBTAINED FROM PLEUROTUS OSTREATUS MUSHROOM",
pages = "37-37",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2367"
}

Matijašević, D., Pavlović, V., Kljajević, N.,& Gardijan, L.. (2024). MORPHOLOGICAL CHANGES OF STAPHYLOCOCCUS AUREUS AND SALMONELLA ENTERITIDIS UPON EXPOSURE TO THE EXTRACT OBTAINED FROM PLEUROTUS OSTREATUS MUSHROOM. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 37-37.
https://hdl.handle.net/21.15107/rcub_imagine_2367

Matijašević D, Pavlović V, Kljajević N, Gardijan L. MORPHOLOGICAL CHANGES OF STAPHYLOCOCCUS AUREUS AND SALMONELLA ENTERITIDIS UPON EXPOSURE TO THE EXTRACT OBTAINED FROM PLEUROTUS OSTREATUS MUSHROOM. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:37-37.
https://hdl.handle.net/21.15107/rcub_imagine_2367 .

Matijašević, Danka, Pavlović, Vladimir, Kljajević, Nemanja, Gardijan, Lazar, "MORPHOLOGICAL CHANGES OF STAPHYLOCOCCUS AUREUS AND SALMONELLA ENTERITIDIS UPON EXPOSURE TO THE EXTRACT OBTAINED FROM PLEUROTUS OSTREATUS MUSHROOM" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):37-37,
https://hdl.handle.net/21.15107/rcub_imagine_2367 .

TY  - JOUR
AU  - Kljajević, Nemanja
AU  - Miloradović, Zorana
AU  - Miočinović, Jelena
AU  - Pavlović, Vladimir
AU  - Tomić, Nikola
AU  - Jovanović, Snežana
PY  - 2023
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/1471-0307.13042
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2328
AB  - Long-term frozen storage of caprine milk is an important way of overcoming seasonal production. White brined cheese, matured for 56 days, was made from frozen-stored caprine milk. Several properties of experimental and control cheese variants were investigated throughout the maturation period: physico-chemical, sensory, proteolytic (by urea-PAGE electrophoresis), texture attributes, microstructure (by scanning electron microscopy). Most of investigated properties were influenced by frozen storage and maturation period, but differences were less pronounced on the 56th day of maturation. Moreover, sensory analysis showed that both cheese variants were well accepted by consumers.
PB  - Wiley
T2  - International Journal of Dairy Technology
T2  - International Journal of Dairy Technology
T1  - Insights into proteolysis, textural evolution, microstructural changes and sensory attributes of white brined cheese from frozen-stored caprine milk
IS  - n/a
VL  - n/a
DO  - 10.1111/1471-0307.13042
ER  - 

@article{
author = "Kljajević, Nemanja and Miloradović, Zorana and Miočinović, Jelena and Pavlović, Vladimir and Tomić, Nikola and Jovanović, Snežana",
year = "2023",
abstract = "Long-term frozen storage of caprine milk is an important way of overcoming seasonal production. White brined cheese, matured for 56 days, was made from frozen-stored caprine milk. Several properties of experimental and control cheese variants were investigated throughout the maturation period: physico-chemical, sensory, proteolytic (by urea-PAGE electrophoresis), texture attributes, microstructure (by scanning electron microscopy). Most of investigated properties were influenced by frozen storage and maturation period, but differences were less pronounced on the 56th day of maturation. Moreover, sensory analysis showed that both cheese variants were well accepted by consumers.",
publisher = "Wiley",
journal = "International Journal of Dairy Technology, International Journal of Dairy Technology",
title = "Insights into proteolysis, textural evolution, microstructural changes and sensory attributes of white brined cheese from frozen-stored caprine milk",
number = "n/a",
volume = "n/a",
doi = "10.1111/1471-0307.13042"
}

Kljajević, N., Miloradović, Z., Miočinović, J., Pavlović, V., Tomić, N.,& Jovanović, S.. (2023). Insights into proteolysis, textural evolution, microstructural changes and sensory attributes of white brined cheese from frozen-stored caprine milk. in International Journal of Dairy Technology
Wiley., n/a(n/a).
https://doi.org/10.1111/1471-0307.13042

Kljajević N, Miloradović Z, Miočinović J, Pavlović V, Tomić N, Jovanović S. Insights into proteolysis, textural evolution, microstructural changes and sensory attributes of white brined cheese from frozen-stored caprine milk. in International Journal of Dairy Technology. 2023;n/a(n/a).
doi:10.1111/1471-0307.13042 .

Kljajević, Nemanja, Miloradović, Zorana, Miočinović, Jelena, Pavlović, Vladimir, Tomić, Nikola, Jovanović, Snežana, "Insights into proteolysis, textural evolution, microstructural changes and sensory attributes of white brined cheese from frozen-stored caprine milk" in International Journal of Dairy Technology, n/a, no. n/a (2023),
https://doi.org/10.1111/1471-0307.13042 . .