TY  - CONF
AU  - Lazić, Stefan
AU  - Stanisavljević Ninković, Danijela
AU  - Petrović, Isidora
AU  - Aleksandra, Medić
AU  - Milivojević, Milena
AU  - Bojić, Luka
AU  - Erceg, Slaven
AU  - Stevanović, Milena
AU  - Švirtlih, Marija
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2287
AB  - Brain trauma leads to the induction of neural stem cell proliferation and the migration
of young neurons to injured areas. However, these neurons are insufficient to fully
restore neuronal function due to the limited potential of adult neurogenesis. This study
aimed to investigate the effect of hypoxia, a condition that underlines a wide spectrum
of brain pathologies, on pluripotency and the capacity of stem cells to differentiate
into neural progenitors. We analyzed the expression of SOX genes and microRNAs as
they control a variety of cellular processes during neuronal differentiation, including
cell proliferation and cell fate determination. In vitro neuronal differentiation of
human embryonal carcinoma cell line NT2/D1 and induced pluripotent stem cells
were used as a model system of adult neurogenesis. Cobalt chloride was used to
induce hypoxia.
The results of the analysis showed that, following hypoxia, the efficiency of neuronal
induction was significantly decreased, that coincident with decline in mRNA
expression levels of SOXB and SOXC genes. In contrast to that, the expression level of
miR-21 was significantly increased.
Our findings advance the study of SOX TFs, miR-21, and their possible interplay in
ischemia-related pathologies, establishing them as prospective biomarkers and
possible targets for future diagnostic and therapeutic approaches.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of the Serbian Neuroscience Society
T1  - Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21
EP  - 96
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2287
ER  - 

@conference{
author = "Lazić, Stefan and Stanisavljević Ninković, Danijela and Petrović, Isidora and Aleksandra, Medić and Milivojević, Milena and Bojić, Luka and Erceg, Slaven and Stevanović, Milena and Švirtlih, Marija",
year = "2023",
abstract = "Brain trauma leads to the induction of neural stem cell proliferation and the migration
of young neurons to injured areas. However, these neurons are insufficient to fully
restore neuronal function due to the limited potential of adult neurogenesis. This study
aimed to investigate the effect of hypoxia, a condition that underlines a wide spectrum
of brain pathologies, on pluripotency and the capacity of stem cells to differentiate
into neural progenitors. We analyzed the expression of SOX genes and microRNAs as
they control a variety of cellular processes during neuronal differentiation, including
cell proliferation and cell fate determination. In vitro neuronal differentiation of
human embryonal carcinoma cell line NT2/D1 and induced pluripotent stem cells
were used as a model system of adult neurogenesis. Cobalt chloride was used to
induce hypoxia.
The results of the analysis showed that, following hypoxia, the efficiency of neuronal
induction was significantly decreased, that coincident with decline in mRNA
expression levels of SOXB and SOXC genes. In contrast to that, the expression level of
miR-21 was significantly increased.
Our findings advance the study of SOX TFs, miR-21, and their possible interplay in
ischemia-related pathologies, establishing them as prospective biomarkers and
possible targets for future diagnostic and therapeutic approaches.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of the Serbian Neuroscience Society",
title = "Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21",
pages = "96-96",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2287"
}

Lazić, S., Stanisavljević Ninković, D., Petrović, I., Aleksandra, M., Milivojević, M., Bojić, L., Erceg, S., Stevanović, M.,& Švirtlih, M.. (2023). Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21. in 8th Congress of the Serbian Neuroscience Society
Belgrade : Serbian Neuroscience Society., 96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2287

Lazić S, Stanisavljević Ninković D, Petrović I, Aleksandra M, Milivojević M, Bojić L, Erceg S, Stevanović M, Švirtlih M. Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21. in 8th Congress of the Serbian Neuroscience Society. 2023;:96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2287 .

Lazić, Stefan, Stanisavljević Ninković, Danijela, Petrović, Isidora, Aleksandra, Medić, Milivojević, Milena, Bojić, Luka, Erceg, Slaven, Stevanović, Milena, Švirtlih, Marija, "Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21" in 8th Congress of the Serbian Neuroscience Society (2023):96-96,
https://hdl.handle.net/21.15107/rcub_imagine_2287 .