TY  - CONF
AU  - Obradović, Bojana
AU  - Stojkovska, Jasmina
AU  - Zvicer, Jovana
AU  - Milivojević, Milena
AU  - Janković, Radmila
AU  - Dragoj, Miodrag
AU  - Jančić, Ivan
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1265
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2365
AB  - Development of novel, effective, and safe anti-tumor drugs is still a slow and cumbersome process, which is often attributed to weaknesses of current preclinical assays and low correlation of the preclinical in vitro and in vivo data with the results obtained in clinical trials. Consequently, there is a clear need for development of more reliable in vitro three dimensional (3D) tumor models, which will capture key features of the in vivo tumor cell microenvironment and provide drug testing results relevant for human patients. The aim of the project “Biomimetic tumor engineering to enhance drug discovery – BioengineeredTumor” funded by the Science Fund of the Republic of Serbia is to develop 2 novel, simple and robust 3D models for cultures of carcinoma and osteosarcoma cells by applying systematic and integrated methodology to comprehensively define the key model components. In specific, the aim is to use different human and animal cancer cell lines in conjunction with alginate-based biomaterials as artificial extracellular matrices imitating tumor environments and to cultivate the obtained constructs in perfusion bioreactors providing enhanced transport of nutrients, gases and biochemical signals to the cells as well as adequate levels of hydrodynamic shear stresses. Thus, the strategic goal is to establish an adaptable platform suited to the use by scientists without technical expertise for long-term in vitro studies of cancer cells for applications in anti-cancer drug discovery and validation, development of personalized medical treatments, and cancer research.
C3  - Hemijska industrija (Chemical Industry)
T1  - Biomimetic tumor engineering to enhance drug discovery - BioengineeredTumor
EP  - 22
IS  - 1S
SP  - 22
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2365
ER  - 

@conference{
author = "Obradović, Bojana and Stojkovska, Jasmina and Zvicer, Jovana and Milivojević, Milena and Janković, Radmila and Dragoj, Miodrag and Jančić, Ivan",
year = "2024",
abstract = "Development of novel, effective, and safe anti-tumor drugs is still a slow and cumbersome process, which is often attributed to weaknesses of current preclinical assays and low correlation of the preclinical in vitro and in vivo data with the results obtained in clinical trials. Consequently, there is a clear need for development of more reliable in vitro three dimensional (3D) tumor models, which will capture key features of the in vivo tumor cell microenvironment and provide drug testing results relevant for human patients. The aim of the project “Biomimetic tumor engineering to enhance drug discovery – BioengineeredTumor” funded by the Science Fund of the Republic of Serbia is to develop 2 novel, simple and robust 3D models for cultures of carcinoma and osteosarcoma cells by applying systematic and integrated methodology to comprehensively define the key model components. In specific, the aim is to use different human and animal cancer cell lines in conjunction with alginate-based biomaterials as artificial extracellular matrices imitating tumor environments and to cultivate the obtained constructs in perfusion bioreactors providing enhanced transport of nutrients, gases and biochemical signals to the cells as well as adequate levels of hydrodynamic shear stresses. Thus, the strategic goal is to establish an adaptable platform suited to the use by scientists without technical expertise for long-term in vitro studies of cancer cells for applications in anti-cancer drug discovery and validation, development of personalized medical treatments, and cancer research.",
journal = "Hemijska industrija (Chemical Industry)",
title = "Biomimetic tumor engineering to enhance drug discovery - BioengineeredTumor",
pages = "22-22",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2365"
}

Obradović, B., Stojkovska, J., Zvicer, J., Milivojević, M., Janković, R., Dragoj, M.,& Jančić, I.. (2024). Biomimetic tumor engineering to enhance drug discovery - BioengineeredTumor. in Hemijska industrija (Chemical Industry), 78(1S), 22-22.
https://hdl.handle.net/21.15107/rcub_imagine_2365

Obradović B, Stojkovska J, Zvicer J, Milivojević M, Janković R, Dragoj M, Jančić I. Biomimetic tumor engineering to enhance drug discovery - BioengineeredTumor. in Hemijska industrija (Chemical Industry). 2024;78(1S):22-22.
https://hdl.handle.net/21.15107/rcub_imagine_2365 .

Obradović, Bojana, Stojkovska, Jasmina, Zvicer, Jovana, Milivojević, Milena, Janković, Radmila, Dragoj, Miodrag, Jančić, Ivan, "Biomimetic tumor engineering to enhance drug discovery - BioengineeredTumor" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):22-22,
https://hdl.handle.net/21.15107/rcub_imagine_2365 .

TY  - CONF
AU  - Petrović, Jelena
AU  - Pańczyszyn, Elżbieta
AU  - Corazzari, Marco
AU  - Banićević, Ivana
AU  - Milivojević, Milena
AU  - Bojić, Luka
AU  - Stevanović, Milena
AU  - Dragoj, Miodrag
AU  - Pešić, Milica
AU  - Janković, Radmila
AU  - Obradović, Bojana
AU  - Stojkovska, Jasmina
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1264
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2364
AB  - The slow advance in anticancer drug development can be attributed to the limitations of conventional models, predominantly monolayer cell (2D) cultures and animal models, which inadequately recapitulate the complex nature of human malignant tumors. Three-dimensional (3D) in vitro models are invaluable tools in drug screening; however, creating a universal model for all cancer types poses challenges due to the diverse nature of cancers. The aim of this work was to develop a single, versatile model using alginate microfibers to accommodate cultivation of various cancer cells.
C3  - Hemijska industrija (Chemical Industry)
T1  - Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model
EP  - 21
IS  - 1S
SP  - 21
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2364
ER  - 

@conference{
author = "Petrović, Jelena and Pańczyszyn, Elżbieta and Corazzari, Marco and Banićević, Ivana and Milivojević, Milena and Bojić, Luka and Stevanović, Milena and Dragoj, Miodrag and Pešić, Milica and Janković, Radmila and Obradović, Bojana and Stojkovska, Jasmina",
year = "2024",
abstract = "The slow advance in anticancer drug development can be attributed to the limitations of conventional models, predominantly monolayer cell (2D) cultures and animal models, which inadequately recapitulate the complex nature of human malignant tumors. Three-dimensional (3D) in vitro models are invaluable tools in drug screening; however, creating a universal model for all cancer types poses challenges due to the diverse nature of cancers. The aim of this work was to develop a single, versatile model using alginate microfibers to accommodate cultivation of various cancer cells.",
journal = "Hemijska industrija (Chemical Industry)",
title = "Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model",
pages = "21-21",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2364"
}

Petrović, J., Pańczyszyn, E., Corazzari, M., Banićević, I., Milivojević, M., Bojić, L., Stevanović, M., Dragoj, M., Pešić, M., Janković, R., Obradović, B.,& Stojkovska, J.. (2024). Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model. in Hemijska industrija (Chemical Industry), 78(1S), 21-21.
https://hdl.handle.net/21.15107/rcub_imagine_2364

Petrović J, Pańczyszyn E, Corazzari M, Banićević I, Milivojević M, Bojić L, Stevanović M, Dragoj M, Pešić M, Janković R, Obradović B, Stojkovska J. Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model. in Hemijska industrija (Chemical Industry). 2024;78(1S):21-21.
https://hdl.handle.net/21.15107/rcub_imagine_2364 .

Petrović, Jelena, Pańczyszyn, Elżbieta, Corazzari, Marco, Banićević, Ivana, Milivojević, Milena, Bojić, Luka, Stevanović, Milena, Dragoj, Miodrag, Pešić, Milica, Janković, Radmila, Obradović, Bojana, Stojkovska, Jasmina, "Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):21-21,
https://hdl.handle.net/21.15107/rcub_imagine_2364 .

TY  - CONF
AU  - Banićević, Ivana
AU  - Milošević, Mia
AU  - Petrović, Jelena
AU  - Menshikh, Ksenia
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Janković, Radmila
AU  - Cochis, Andrea
AU  - Bella, Elena Della
AU  - Stoddart, Martin
AU  - Rimondini, Lia
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1261
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2363
AB  - Comprehensive research, particularly in evaluating drug efficacy, still heavily relies on the results obtained by the utilization of cell monolayers and animals. However, the inherent limitations of these models such as their physiological disparities from humans pose significant obstacles to acquiring reliable results thus impeding further scientific progression. To address this challenge, 3D in vitro cell culture models emerged as physiologically relevant models having the potential to enhance research and drug discovery. Our study aimed to develop a 3D in vitro cell culture model based on bone-like scaffolds in conjunction with a perfusion bioreactor (“3D Perfuse”, Innovation Center FTM, Belgrade, Serbia) for studying both physiological and pathological (i.e. tumors) bone conditions.
C3  - Hemijska industrija (Chemical Industry)
T1  - A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research
EP  - 19
IS  - 1S
SP  - 19
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2363
ER  - 

@conference{
author = "Banićević, Ivana and Milošević, Mia and Petrović, Jelena and Menshikh, Ksenia and Milivojević, Milena and Stevanović, Milena and Janković, Radmila and Cochis, Andrea and Bella, Elena Della and Stoddart, Martin and Rimondini, Lia and Stojkovska, Jasmina and Obradović, Bojana",
year = "2024",
abstract = "Comprehensive research, particularly in evaluating drug efficacy, still heavily relies on the results obtained by the utilization of cell monolayers and animals. However, the inherent limitations of these models such as their physiological disparities from humans pose significant obstacles to acquiring reliable results thus impeding further scientific progression. To address this challenge, 3D in vitro cell culture models emerged as physiologically relevant models having the potential to enhance research and drug discovery. Our study aimed to develop a 3D in vitro cell culture model based on bone-like scaffolds in conjunction with a perfusion bioreactor (“3D Perfuse”, Innovation Center FTM, Belgrade, Serbia) for studying both physiological and pathological (i.e. tumors) bone conditions.",
journal = "Hemijska industrija (Chemical Industry)",
title = "A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research",
pages = "19-19",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2363"
}

Banićević, I., Milošević, M., Petrović, J., Menshikh, K., Milivojević, M., Stevanović, M., Janković, R., Cochis, A., Bella, E. D., Stoddart, M., Rimondini, L., Stojkovska, J.,& Obradović, B.. (2024). A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research. in Hemijska industrija (Chemical Industry), 78(1S), 19-19.
https://hdl.handle.net/21.15107/rcub_imagine_2363

Banićević I, Milošević M, Petrović J, Menshikh K, Milivojević M, Stevanović M, Janković R, Cochis A, Bella ED, Stoddart M, Rimondini L, Stojkovska J, Obradović B. A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research. in Hemijska industrija (Chemical Industry). 2024;78(1S):19-19.
https://hdl.handle.net/21.15107/rcub_imagine_2363 .

Banićević, Ivana, Milošević, Mia, Petrović, Jelena, Menshikh, Ksenia, Milivojević, Milena, Stevanović, Milena, Janković, Radmila, Cochis, Andrea, Bella, Elena Della, Stoddart, Martin, Rimondini, Lia, Stojkovska, Jasmina, Obradović, Bojana, "A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):19-19,
https://hdl.handle.net/21.15107/rcub_imagine_2363 .

TY  - CONF
AU  - Pantović, Isidora
AU  - Živić, Katarina
AU  - Boljević, Ivana
AU  - Nedeljković, Milica
AU  - Janković, Radmila
AU  - Tanić, Miljana
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2046
AB  - Serbia has one of the world’s highest incidences and mortality rates of ovarian cancer.
Germline or somatic mutations in BRCA1 and BRCA2 genes, such as single nucleotide
variants (SNVs), indels, insertions, deletions, commonly lead to development of breast
and ovary cancer. Targeted therapy with PARP inhibitors is the current standard of care for
serous epithelial BRCA-mutated ovarian cancer and depends on the accurate detection of
mutations in these genes.
In this study, a subset of patient specimens from Institute of Oncology and Radiology
were sequenced on MiSeq Illumina sequencer, raw data were analysed bioinformatically,
which included checking quality control of raw FASTQ sequences, trimming, mapping
them on reference genome(hg19), target coverage quality control and variant calling. We
tested various variant calling tools including Mutect2, GATK HaplotypeCaller, FreeBayes,
VarDict and MuSe callers. We evaluated the relative performance- concordance rate, false
positive and false negative rates between the callers for SNV/indel detection in BRCA1
and BRCA2 genes.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Evaluation of variant calling tools for detection of SNVs in BRCA1 and BRCA2 genes in patients from the Institute of Oncology and Radiology of Serbia
EP  - 101
SP  - 101
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2046
ER  - 

@conference{
author = "Pantović, Isidora and Živić, Katarina and Boljević, Ivana and Nedeljković, Milica and Janković, Radmila and Tanić, Miljana",
year = "2023",
abstract = "Serbia has one of the world’s highest incidences and mortality rates of ovarian cancer.
Germline or somatic mutations in BRCA1 and BRCA2 genes, such as single nucleotide
variants (SNVs), indels, insertions, deletions, commonly lead to development of breast
and ovary cancer. Targeted therapy with PARP inhibitors is the current standard of care for
serous epithelial BRCA-mutated ovarian cancer and depends on the accurate detection of
mutations in these genes.
In this study, a subset of patient specimens from Institute of Oncology and Radiology
were sequenced on MiSeq Illumina sequencer, raw data were analysed bioinformatically,
which included checking quality control of raw FASTQ sequences, trimming, mapping
them on reference genome(hg19), target coverage quality control and variant calling. We
tested various variant calling tools including Mutect2, GATK HaplotypeCaller, FreeBayes,
VarDict and MuSe callers. We evaluated the relative performance- concordance rate, false
positive and false negative rates between the callers for SNV/indel detection in BRCA1
and BRCA2 genes.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Evaluation of variant calling tools for detection of SNVs in BRCA1 and BRCA2 genes in patients from the Institute of Oncology and Radiology of Serbia",
pages = "101-101",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2046"
}

Pantović, I., Živić, K., Boljević, I., Nedeljković, M., Janković, R.,& Tanić, M.. (2023). Evaluation of variant calling tools for detection of SNVs in BRCA1 and BRCA2 genes in patients from the Institute of Oncology and Radiology of Serbia. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 101-101.
https://hdl.handle.net/21.15107/rcub_imagine_2046

Pantović I, Živić K, Boljević I, Nedeljković M, Janković R, Tanić M. Evaluation of variant calling tools for detection of SNVs in BRCA1 and BRCA2 genes in patients from the Institute of Oncology and Radiology of Serbia. in 4th Belgrade Bioinformatics Conference. 2023;4:101-101.
https://hdl.handle.net/21.15107/rcub_imagine_2046 .

Pantović, Isidora, Živić, Katarina, Boljević, Ivana, Nedeljković, Milica, Janković, Radmila, Tanić, Miljana, "Evaluation of variant calling tools for detection of SNVs in BRCA1 and BRCA2 genes in patients from the Institute of Oncology and Radiology of Serbia" in 4th Belgrade Bioinformatics Conference, 4 (2023):101-101,
https://hdl.handle.net/21.15107/rcub_imagine_2046 .

TY  - JOUR
AU  - Jovanović, Ljubiša
AU  - Nikolić, Aleksandra
AU  - Dragičević, Sandra
AU  - Jović, Milena
AU  - Janković, Radmila
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2300
AB  - Aim To analyze the expression of autophagy markers p62,
LC3, and Beclin1 in ovarian cancer tissue and evaluate the
prognostic potential of these markers.
Methods The study enrolled 328 patients: 122 with epithelial ovarian carcinoma, 42 with atypical proliferative tumor, and 164 with benign epithelial ovarian tumor. The expression of p62, LC3, and Beclin1 was analyzed in central
and invasive tumor segments with immunohistochemistry
combined with tissue microarray. The expression levels of
the analyzed markers were correlated with relevant histopathology parameters.
Results The expression of all analyzed markers was most
remarkable in epithelial ovarian carcinoma. There was a
strong positive correlation between the expressions of p62
and LC3, while these two markers negatively correlated
with Beclin1. High-grade serous carcinoma had higher p62
and LC3 levels, and lower Beclin1 levels than other tumor
types. This expression profile was also observed in more
advanced tumor stages.
Conclusion Prominent p62 and LC3 expression in combination with weak Beclin1 expression in high-grade serous
carcinoma indicates potential for the application of autophagy inhibitors in patients with this tumor subtype.
PB  - Medicinska naklada (Croatia)
T2  - Croatian Medical Journal
T1  - Prognostic relevance of autophagy-related markers p62, LC3, and Beclin1 in ovarian cancer
EP  - 460
IS  - 5
SP  - 453
VL  - 63
DO  - 10.3325/cmj.2022.63.453
ER  - 

@article{
author = "Jovanović, Ljubiša and Nikolić, Aleksandra and Dragičević, Sandra and Jović, Milena and Janković, Radmila",
year = "2022",
abstract = "Aim To analyze the expression of autophagy markers p62,
LC3, and Beclin1 in ovarian cancer tissue and evaluate the
prognostic potential of these markers.
Methods The study enrolled 328 patients: 122 with epithelial ovarian carcinoma, 42 with atypical proliferative tumor, and 164 with benign epithelial ovarian tumor. The expression of p62, LC3, and Beclin1 was analyzed in central
and invasive tumor segments with immunohistochemistry
combined with tissue microarray. The expression levels of
the analyzed markers were correlated with relevant histopathology parameters.
Results The expression of all analyzed markers was most
remarkable in epithelial ovarian carcinoma. There was a
strong positive correlation between the expressions of p62
and LC3, while these two markers negatively correlated
with Beclin1. High-grade serous carcinoma had higher p62
and LC3 levels, and lower Beclin1 levels than other tumor
types. This expression profile was also observed in more
advanced tumor stages.
Conclusion Prominent p62 and LC3 expression in combination with weak Beclin1 expression in high-grade serous
carcinoma indicates potential for the application of autophagy inhibitors in patients with this tumor subtype.",
publisher = "Medicinska naklada (Croatia)",
journal = "Croatian Medical Journal",
title = "Prognostic relevance of autophagy-related markers p62, LC3, and Beclin1 in ovarian cancer",
pages = "460-453",
number = "5",
volume = "63",
doi = "10.3325/cmj.2022.63.453"
}

Jovanović, L., Nikolić, A., Dragičević, S., Jović, M.,& Janković, R.. (2022). Prognostic relevance of autophagy-related markers p62, LC3, and Beclin1 in ovarian cancer. in Croatian Medical Journal
Medicinska naklada (Croatia)., 63(5), 453-460.
https://doi.org/10.3325/cmj.2022.63.453

Jovanović L, Nikolić A, Dragičević S, Jović M, Janković R. Prognostic relevance of autophagy-related markers p62, LC3, and Beclin1 in ovarian cancer. in Croatian Medical Journal. 2022;63(5):453-460.
doi:10.3325/cmj.2022.63.453 .

Jovanović, Ljubiša, Nikolić, Aleksandra, Dragičević, Sandra, Jović, Milena, Janković, Radmila, "Prognostic relevance of autophagy-related markers p62, LC3, and Beclin1 in ovarian cancer" in Croatian Medical Journal, 63, no. 5 (2022):453-460,
https://doi.org/10.3325/cmj.2022.63.453 . .

TY  - CHAP
AU  - Ristić, Nina
AU  - Išić Denčić, Tijana
AU  - Janković, Radmila
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1715
AB  - Ćelije imunskog sistema imaju važnu ulogu u očuvanju barijere gastrointestinalnog trakta. Savremeni način
života, izloženost različitim alergenima i genetska predispozicija mogu da dovedu do narušavanja odnosa i
broja ćelija imunskog sistema, čime se remeti homeostaza na nivou crevne sluznice i omogućava razvoj bolesti.
Povećanje broja eozinofilnih leukocita u sluznici i narušavanje barijere gastrointestinalnog trakta karakteristika
je različitih primarnih i sekundarnih eozinofilnih gastrointestinalnih bolesti (EGIB). Usled čestog preklapanja
u histološkom nalazu klinički različitih entiteta (kao što su eozinofilni ezofagitis, gastroezofagusna
refluksna bolest i eozinofilni gastroentritis) postoji konstantna potreba za otkrivanjem novih markera diferencijacije,
kao i veze između kliničkih karakteristika i patofizioloških mehanizama bolesti.
Identifikacija EGIB transkriptoma i razlučivanje imunopatogeneze različitih EGIB oboljenja omogućava identifikaciju
novih molekularnih markera. Molekularno profilisanje gena specifičnih za određeno oboljenje
poboljšava dijagnozu i kliničko praćenje, omogućava brz odabir adekvatnog terapijskog pristupa, kao i personalizovani
medicinski pristup obolelima. Iako postoji značajan broj studija koje se bave validacijom novih
lekova kod EGIB obolelih, većina njih je još uvek u vidu preliminarnih istraživanja, te je neophodna dalja verifikacija
efekata ispitivanih lekova u kliničkoj praksi.
AB  - The cells of the immune system play an important role in preserving the barrier of the gastrointestinal tract.
Modern lifestyle, exposure to various allergens and genetic predisposition can lead to a disruption of the relationship
and the number of cells of the immune system, which disturb homeostasis at the level of the intestinal
mucosa and enables the development of the disease.
An increase in the number of eosinophilic leukocytes in the mucosa and disruption of the gastrointestinal
tract barrier is a characteristic of various primary and secondary eosinophilic gastrointestinal diseases (EGIB).
Due to the frequent overlap in the histological findings of clinically different entities (such as eosinophilic
esophagitis, gastroesophageal reflux disease and eosinophilic gastroentritis), there is a constant need to
discover new markers of differentiation, as well as the relationship between clinical characteristics and pathophysiological
mechanisms of the disease.
Identification of EGIB transcriptoma and differentiation of immunopathogenesis of different EGIB diseases
enables identification of new molecular markers. Molecular profiling of disease-specific genes improves diagnosis
and clinical monitoring, enables rapid selection of an adequate therapeutic approach, as well as a personalized
medical approach to patients. Although there are a significant number of studies regarding the
validation of new drugs in EGIB patients, most of them are still in the form of preliminary research, and further
verification of the effects of the tested drugs in clinical practice is necessary.
PB  - Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo
T2  - Trendovi u molekularnoj Biologiji
T1  - Diferencijalna dijagnoza eozinofilnog infiltrata u sluznici jednjaka primenom molekularno-bioloških metoda
T1  - Differential diagnosis of eosinophilic infiltrate in esophageal mucosa by applying molecular biology method
EP  - 106
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1715
ER  - 

@inbook{
author = "Ristić, Nina and Išić Denčić, Tijana and Janković, Radmila",
year = "2021",
abstract = "Ćelije imunskog sistema imaju važnu ulogu u očuvanju barijere gastrointestinalnog trakta. Savremeni način
života, izloženost različitim alergenima i genetska predispozicija mogu da dovedu do narušavanja odnosa i
broja ćelija imunskog sistema, čime se remeti homeostaza na nivou crevne sluznice i omogućava razvoj bolesti.
Povećanje broja eozinofilnih leukocita u sluznici i narušavanje barijere gastrointestinalnog trakta karakteristika
je različitih primarnih i sekundarnih eozinofilnih gastrointestinalnih bolesti (EGIB). Usled čestog preklapanja
u histološkom nalazu klinički različitih entiteta (kao što su eozinofilni ezofagitis, gastroezofagusna
refluksna bolest i eozinofilni gastroentritis) postoji konstantna potreba za otkrivanjem novih markera diferencijacije,
kao i veze između kliničkih karakteristika i patofizioloških mehanizama bolesti.
Identifikacija EGIB transkriptoma i razlučivanje imunopatogeneze različitih EGIB oboljenja omogućava identifikaciju
novih molekularnih markera. Molekularno profilisanje gena specifičnih za određeno oboljenje
poboljšava dijagnozu i kliničko praćenje, omogućava brz odabir adekvatnog terapijskog pristupa, kao i personalizovani
medicinski pristup obolelima. Iako postoji značajan broj studija koje se bave validacijom novih
lekova kod EGIB obolelih, većina njih je još uvek u vidu preliminarnih istraživanja, te je neophodna dalja verifikacija
efekata ispitivanih lekova u kliničkoj praksi., The cells of the immune system play an important role in preserving the barrier of the gastrointestinal tract.
Modern lifestyle, exposure to various allergens and genetic predisposition can lead to a disruption of the relationship
and the number of cells of the immune system, which disturb homeostasis at the level of the intestinal
mucosa and enables the development of the disease.
An increase in the number of eosinophilic leukocytes in the mucosa and disruption of the gastrointestinal
tract barrier is a characteristic of various primary and secondary eosinophilic gastrointestinal diseases (EGIB).
Due to the frequent overlap in the histological findings of clinically different entities (such as eosinophilic
esophagitis, gastroesophageal reflux disease and eosinophilic gastroentritis), there is a constant need to
discover new markers of differentiation, as well as the relationship between clinical characteristics and pathophysiological
mechanisms of the disease.
Identification of EGIB transcriptoma and differentiation of immunopathogenesis of different EGIB diseases
enables identification of new molecular markers. Molecular profiling of disease-specific genes improves diagnosis
and clinical monitoring, enables rapid selection of an adequate therapeutic approach, as well as a personalized
medical approach to patients. Although there are a significant number of studies regarding the
validation of new drugs in EGIB patients, most of them are still in the form of preliminary research, and further
verification of the effects of the tested drugs in clinical practice is necessary.",
publisher = "Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo",
journal = "Trendovi u molekularnoj Biologiji",
booktitle = "Diferencijalna dijagnoza eozinofilnog infiltrata u sluznici jednjaka primenom molekularno-bioloških metoda, Differential diagnosis of eosinophilic infiltrate in esophageal mucosa by applying molecular biology method",
pages = "106-96",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1715"
}

Ristić, N., Išić Denčić, T.,& Janković, R.. (2021). Diferencijalna dijagnoza eozinofilnog infiltrata u sluznici jednjaka primenom molekularno-bioloških metoda. in Trendovi u molekularnoj Biologiji
Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo., 96-106.
https://hdl.handle.net/21.15107/rcub_imagine_1715

Ristić N, Išić Denčić T, Janković R. Diferencijalna dijagnoza eozinofilnog infiltrata u sluznici jednjaka primenom molekularno-bioloških metoda. in Trendovi u molekularnoj Biologiji. 2021;:96-106.
https://hdl.handle.net/21.15107/rcub_imagine_1715 .

Ristić, Nina, Išić Denčić, Tijana, Janković, Radmila, "Diferencijalna dijagnoza eozinofilnog infiltrata u sluznici jednjaka primenom molekularno-bioloških metoda" in Trendovi u molekularnoj Biologiji (2021):96-106,
https://hdl.handle.net/21.15107/rcub_imagine_1715 .