TY  - CONF
AU  - Petrović, Jelena
AU  - Pańczyszyn, Elżbieta
AU  - Corazzari, Marco
AU  - Banićević, Ivana
AU  - Milivojević, Milena
AU  - Bojić, Luka
AU  - Stevanović, Milena
AU  - Dragoj, Miodrag
AU  - Pešić, Milica
AU  - Janković, Radmila
AU  - Obradović, Bojana
AU  - Stojkovska, Jasmina
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1264
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2364
AB  - The slow advance in anticancer drug development can be attributed to the limitations of conventional models, predominantly monolayer cell (2D) cultures and animal models, which inadequately recapitulate the complex nature of human malignant tumors. Three-dimensional (3D) in vitro models are invaluable tools in drug screening; however, creating a universal model for all cancer types poses challenges due to the diverse nature of cancers. The aim of this work was to develop a single, versatile model using alginate microfibers to accommodate cultivation of various cancer cells.
C3  - Hemijska industrija (Chemical Industry)
T1  - Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model
EP  - 21
IS  - 1S
SP  - 21
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2364
ER  - 

@conference{
author = "Petrović, Jelena and Pańczyszyn, Elżbieta and Corazzari, Marco and Banićević, Ivana and Milivojević, Milena and Bojić, Luka and Stevanović, Milena and Dragoj, Miodrag and Pešić, Milica and Janković, Radmila and Obradović, Bojana and Stojkovska, Jasmina",
year = "2024",
abstract = "The slow advance in anticancer drug development can be attributed to the limitations of conventional models, predominantly monolayer cell (2D) cultures and animal models, which inadequately recapitulate the complex nature of human malignant tumors. Three-dimensional (3D) in vitro models are invaluable tools in drug screening; however, creating a universal model for all cancer types poses challenges due to the diverse nature of cancers. The aim of this work was to develop a single, versatile model using alginate microfibers to accommodate cultivation of various cancer cells.",
journal = "Hemijska industrija (Chemical Industry)",
title = "Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model",
pages = "21-21",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2364"
}

Petrović, J., Pańczyszyn, E., Corazzari, M., Banićević, I., Milivojević, M., Bojić, L., Stevanović, M., Dragoj, M., Pešić, M., Janković, R., Obradović, B.,& Stojkovska, J.. (2024). Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model. in Hemijska industrija (Chemical Industry), 78(1S), 21-21.
https://hdl.handle.net/21.15107/rcub_imagine_2364

Petrović J, Pańczyszyn E, Corazzari M, Banićević I, Milivojević M, Bojić L, Stevanović M, Dragoj M, Pešić M, Janković R, Obradović B, Stojkovska J. Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model. in Hemijska industrija (Chemical Industry). 2024;78(1S):21-21.
https://hdl.handle.net/21.15107/rcub_imagine_2364 .

Petrović, Jelena, Pańczyszyn, Elżbieta, Corazzari, Marco, Banićević, Ivana, Milivojević, Milena, Bojić, Luka, Stevanović, Milena, Dragoj, Miodrag, Pešić, Milica, Janković, Radmila, Obradović, Bojana, Stojkovska, Jasmina, "Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):21-21,
https://hdl.handle.net/21.15107/rcub_imagine_2364 .

TY  - CONF
AU  - Banićević, Ivana
AU  - Milošević, Mia
AU  - Petrović, Jelena
AU  - Menshikh, Ksenia
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Janković, Radmila
AU  - Cochis, Andrea
AU  - Bella, Elena Della
AU  - Stoddart, Martin
AU  - Rimondini, Lia
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1261
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2363
AB  - Comprehensive research, particularly in evaluating drug efficacy, still heavily relies on the results obtained by the utilization of cell monolayers and animals. However, the inherent limitations of these models such as their physiological disparities from humans pose significant obstacles to acquiring reliable results thus impeding further scientific progression. To address this challenge, 3D in vitro cell culture models emerged as physiologically relevant models having the potential to enhance research and drug discovery. Our study aimed to develop a 3D in vitro cell culture model based on bone-like scaffolds in conjunction with a perfusion bioreactor (“3D Perfuse”, Innovation Center FTM, Belgrade, Serbia) for studying both physiological and pathological (i.e. tumors) bone conditions.
C3  - Hemijska industrija (Chemical Industry)
T1  - A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research
EP  - 19
IS  - 1S
SP  - 19
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2363
ER  - 

@conference{
author = "Banićević, Ivana and Milošević, Mia and Petrović, Jelena and Menshikh, Ksenia and Milivojević, Milena and Stevanović, Milena and Janković, Radmila and Cochis, Andrea and Bella, Elena Della and Stoddart, Martin and Rimondini, Lia and Stojkovska, Jasmina and Obradović, Bojana",
year = "2024",
abstract = "Comprehensive research, particularly in evaluating drug efficacy, still heavily relies on the results obtained by the utilization of cell monolayers and animals. However, the inherent limitations of these models such as their physiological disparities from humans pose significant obstacles to acquiring reliable results thus impeding further scientific progression. To address this challenge, 3D in vitro cell culture models emerged as physiologically relevant models having the potential to enhance research and drug discovery. Our study aimed to develop a 3D in vitro cell culture model based on bone-like scaffolds in conjunction with a perfusion bioreactor (“3D Perfuse”, Innovation Center FTM, Belgrade, Serbia) for studying both physiological and pathological (i.e. tumors) bone conditions.",
journal = "Hemijska industrija (Chemical Industry)",
title = "A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research",
pages = "19-19",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2363"
}

Banićević, I., Milošević, M., Petrović, J., Menshikh, K., Milivojević, M., Stevanović, M., Janković, R., Cochis, A., Bella, E. D., Stoddart, M., Rimondini, L., Stojkovska, J.,& Obradović, B.. (2024). A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research. in Hemijska industrija (Chemical Industry), 78(1S), 19-19.
https://hdl.handle.net/21.15107/rcub_imagine_2363

Banićević I, Milošević M, Petrović J, Menshikh K, Milivojević M, Stevanović M, Janković R, Cochis A, Bella ED, Stoddart M, Rimondini L, Stojkovska J, Obradović B. A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research. in Hemijska industrija (Chemical Industry). 2024;78(1S):19-19.
https://hdl.handle.net/21.15107/rcub_imagine_2363 .

Banićević, Ivana, Milošević, Mia, Petrović, Jelena, Menshikh, Ksenia, Milivojević, Milena, Stevanović, Milena, Janković, Radmila, Cochis, Andrea, Bella, Elena Della, Stoddart, Martin, Rimondini, Lia, Stojkovska, Jasmina, Obradović, Bojana, "A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):19-19,
https://hdl.handle.net/21.15107/rcub_imagine_2363 .

TY  - CONF
AU  - Bojić, Luka
AU  - Pejić, Jelena
AU  - Stojkovska, Jasmina
AU  - Stevanović, Milena
AU  - Medić, Aleksandra
AU  - Petrović, Isidora
AU  - Milivojević, Milena
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1262
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2366
AB  - Osteosarcoma (OS) is a highly aggressive primary malignant bone tumor that most commonly affects children, adolescents, and young adults. The standard treatment for OS consists of surgical resection and chemotherapy, whereas radiation therapy is recommended for the unresectable tumor. Due to its easy metastasis and recurrence, the 5-year overall survival rate is only 66.5 %. Thus, there is a critical need to recognize the molecular mechanisms underlying OS development and pathogenesis. Traditionally, two-dimensional (2D) cells are widely used in cancer biology and pre-clinical studies. However, 2D models are unable to mimic cell–cell and cell-extracellular matrix interactions which are crucial for adequate cellular function. Three-dimensional (3D) model systems are able to recapitulate key features of human cancer and are recognized as a promising platform for fundamental and translational research. In the present work, we established an osteosarcoma 3D model based on alginate microbeads and studied the effect of combined treatment with doxorubicin (Doxo), widely used chemotherapeutic, and quercetin (Quer), a plant pigment with anticancer properties, on OS model systems.
C3  - Hemijska industrija (Chemical Industry)
T1  - Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems
EP  - 20
IS  - 1S
SP  - 20
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2366
ER  - 

@conference{
author = "Bojić, Luka and Pejić, Jelena and Stojkovska, Jasmina and Stevanović, Milena and Medić, Aleksandra and Petrović, Isidora and Milivojević, Milena",
year = "2024",
abstract = "Osteosarcoma (OS) is a highly aggressive primary malignant bone tumor that most commonly affects children, adolescents, and young adults. The standard treatment for OS consists of surgical resection and chemotherapy, whereas radiation therapy is recommended for the unresectable tumor. Due to its easy metastasis and recurrence, the 5-year overall survival rate is only 66.5 %. Thus, there is a critical need to recognize the molecular mechanisms underlying OS development and pathogenesis. Traditionally, two-dimensional (2D) cells are widely used in cancer biology and pre-clinical studies. However, 2D models are unable to mimic cell–cell and cell-extracellular matrix interactions which are crucial for adequate cellular function. Three-dimensional (3D) model systems are able to recapitulate key features of human cancer and are recognized as a promising platform for fundamental and translational research. In the present work, we established an osteosarcoma 3D model based on alginate microbeads and studied the effect of combined treatment with doxorubicin (Doxo), widely used chemotherapeutic, and quercetin (Quer), a plant pigment with anticancer properties, on OS model systems.",
journal = "Hemijska industrija (Chemical Industry)",
title = "Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems",
pages = "20-20",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2366"
}

Bojić, L., Pejić, J., Stojkovska, J., Stevanović, M., Medić, A., Petrović, I.,& Milivojević, M.. (2024). Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems. in Hemijska industrija (Chemical Industry), 78(1S), 20-20.
https://hdl.handle.net/21.15107/rcub_imagine_2366

Bojić L, Pejić J, Stojkovska J, Stevanović M, Medić A, Petrović I, Milivojević M. Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems. in Hemijska industrija (Chemical Industry). 2024;78(1S):20-20.
https://hdl.handle.net/21.15107/rcub_imagine_2366 .

Bojić, Luka, Pejić, Jelena, Stojkovska, Jasmina, Stevanović, Milena, Medić, Aleksandra, Petrović, Isidora, Milivojević, Milena, "Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):20-20,
https://hdl.handle.net/21.15107/rcub_imagine_2366 .

TY  - CONF
AU  - Obradović, Bojana
AU  - Stojkovska, Jasmina
AU  - Zvicer, Jovana
AU  - Milivojević, Milena
AU  - Janković, Radmila
AU  - Dragoj, Miodrag
AU  - Jančić, Ivan
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1265
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2365
AB  - Development of novel, effective, and safe anti-tumor drugs is still a slow and cumbersome process, which is often attributed to weaknesses of current preclinical assays and low correlation of the preclinical in vitro and in vivo data with the results obtained in clinical trials. Consequently, there is a clear need for development of more reliable in vitro three dimensional (3D) tumor models, which will capture key features of the in vivo tumor cell microenvironment and provide drug testing results relevant for human patients. The aim of the project “Biomimetic tumor engineering to enhance drug discovery – BioengineeredTumor” funded by the Science Fund of the Republic of Serbia is to develop 2 novel, simple and robust 3D models for cultures of carcinoma and osteosarcoma cells by applying systematic and integrated methodology to comprehensively define the key model components. In specific, the aim is to use different human and animal cancer cell lines in conjunction with alginate-based biomaterials as artificial extracellular matrices imitating tumor environments and to cultivate the obtained constructs in perfusion bioreactors providing enhanced transport of nutrients, gases and biochemical signals to the cells as well as adequate levels of hydrodynamic shear stresses. Thus, the strategic goal is to establish an adaptable platform suited to the use by scientists without technical expertise for long-term in vitro studies of cancer cells for applications in anti-cancer drug discovery and validation, development of personalized medical treatments, and cancer research.
C3  - Hemijska industrija (Chemical Industry)
T1  - Biomimetic tumor engineering to enhance drug discovery - BioengineeredTumor
EP  - 22
IS  - 1S
SP  - 22
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2365
ER  - 

@conference{
author = "Obradović, Bojana and Stojkovska, Jasmina and Zvicer, Jovana and Milivojević, Milena and Janković, Radmila and Dragoj, Miodrag and Jančić, Ivan",
year = "2024",
abstract = "Development of novel, effective, and safe anti-tumor drugs is still a slow and cumbersome process, which is often attributed to weaknesses of current preclinical assays and low correlation of the preclinical in vitro and in vivo data with the results obtained in clinical trials. Consequently, there is a clear need for development of more reliable in vitro three dimensional (3D) tumor models, which will capture key features of the in vivo tumor cell microenvironment and provide drug testing results relevant for human patients. The aim of the project “Biomimetic tumor engineering to enhance drug discovery – BioengineeredTumor” funded by the Science Fund of the Republic of Serbia is to develop 2 novel, simple and robust 3D models for cultures of carcinoma and osteosarcoma cells by applying systematic and integrated methodology to comprehensively define the key model components. In specific, the aim is to use different human and animal cancer cell lines in conjunction with alginate-based biomaterials as artificial extracellular matrices imitating tumor environments and to cultivate the obtained constructs in perfusion bioreactors providing enhanced transport of nutrients, gases and biochemical signals to the cells as well as adequate levels of hydrodynamic shear stresses. Thus, the strategic goal is to establish an adaptable platform suited to the use by scientists without technical expertise for long-term in vitro studies of cancer cells for applications in anti-cancer drug discovery and validation, development of personalized medical treatments, and cancer research.",
journal = "Hemijska industrija (Chemical Industry)",
title = "Biomimetic tumor engineering to enhance drug discovery - BioengineeredTumor",
pages = "22-22",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2365"
}

Obradović, B., Stojkovska, J., Zvicer, J., Milivojević, M., Janković, R., Dragoj, M.,& Jančić, I.. (2024). Biomimetic tumor engineering to enhance drug discovery - BioengineeredTumor. in Hemijska industrija (Chemical Industry), 78(1S), 22-22.
https://hdl.handle.net/21.15107/rcub_imagine_2365

Obradović B, Stojkovska J, Zvicer J, Milivojević M, Janković R, Dragoj M, Jančić I. Biomimetic tumor engineering to enhance drug discovery - BioengineeredTumor. in Hemijska industrija (Chemical Industry). 2024;78(1S):22-22.
https://hdl.handle.net/21.15107/rcub_imagine_2365 .

Obradović, Bojana, Stojkovska, Jasmina, Zvicer, Jovana, Milivojević, Milena, Janković, Radmila, Dragoj, Miodrag, Jančić, Ivan, "Biomimetic tumor engineering to enhance drug discovery - BioengineeredTumor" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):22-22,
https://hdl.handle.net/21.15107/rcub_imagine_2365 .

TY  - CONF
AU  - Pejić, Jelena
AU  - Mojsin, Marija
AU  - Stojkovska, Jasmina
AU  - Medić, Aleksandra
AU  - Petrović, Isidora
AU  - Stevanović, Milena
AU  - Obradović, Bojana
AU  - Milivojević, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2151
AB  - Introduction: The NT2/D1 embryonal carcinoma cell line represents a well-established in vitro model of
human neurogenesis. It’s widely used for studying neurodevelopmental processes, neurotoxicity, and
neurodegenerative disorders. The utilization of alginate fibers as a 3D cell culture system offers a biocompatible and structurally supportive environment for neural differentiation and maturation of cells,
making it a suitable tool for investigating neurodevelopmental processes.
Methods: In thisstudy, we evaluated the alginate microfibers as a 3D modelsystem for in vitro neural differentiation of NT2/D1 cells.We described the immobilization of NT2/D1 cellsin alginate microfibers and
the effect of propagation in this 3D model on morphological features, viability, and proliferation of immobilized cells. We also assessed the RA-induced initiation of neural differentiation of NT2/D1 cellsin alginate microfibers by comparison with the initiation of neural differentiation in adherent 2D cell culture.
Results: Our results showed that immobilized NT2/D1 acquired morphological features characteristic
of cells propagated in 3D model systems and retain viability, proliferative capacity, and ability to attach
to adherent surfaces. In addition, immobilized NT2/D1 cells preserved neural differentiation capacity.
Upon RA induction we detected a marked decrease in the expression of specific pluripotency-maintaining markers, SOX2, OCT4, and NANOG. Consecutively, the expression of early neural markers, SOX3,
PAX6, and miR219 was significantly increased.
Conclusion: Neural differentiation of NT2/D1 cellsimmobilized within alginate fibersrepresents a highly
promising 3D modelsystem forstudying human neurogenesis and offers a valuable platform forscreening the effect of drugs and bioactive compounds on human neural differentiation.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds
EP  - 113
SP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2151
ER  - 

@conference{
author = "Pejić, Jelena and Mojsin, Marija and Stojkovska, Jasmina and Medić, Aleksandra and Petrović, Isidora and Stevanović, Milena and Obradović, Bojana and Milivojević, Milena",
year = "2023",
abstract = "Introduction: The NT2/D1 embryonal carcinoma cell line represents a well-established in vitro model of
human neurogenesis. It’s widely used for studying neurodevelopmental processes, neurotoxicity, and
neurodegenerative disorders. The utilization of alginate fibers as a 3D cell culture system offers a biocompatible and structurally supportive environment for neural differentiation and maturation of cells,
making it a suitable tool for investigating neurodevelopmental processes.
Methods: In thisstudy, we evaluated the alginate microfibers as a 3D modelsystem for in vitro neural differentiation of NT2/D1 cells.We described the immobilization of NT2/D1 cellsin alginate microfibers and
the effect of propagation in this 3D model on morphological features, viability, and proliferation of immobilized cells. We also assessed the RA-induced initiation of neural differentiation of NT2/D1 cellsin alginate microfibers by comparison with the initiation of neural differentiation in adherent 2D cell culture.
Results: Our results showed that immobilized NT2/D1 acquired morphological features characteristic
of cells propagated in 3D model systems and retain viability, proliferative capacity, and ability to attach
to adherent surfaces. In addition, immobilized NT2/D1 cells preserved neural differentiation capacity.
Upon RA induction we detected a marked decrease in the expression of specific pluripotency-maintaining markers, SOX2, OCT4, and NANOG. Consecutively, the expression of early neural markers, SOX3,
PAX6, and miR219 was significantly increased.
Conclusion: Neural differentiation of NT2/D1 cellsimmobilized within alginate fibersrepresents a highly
promising 3D modelsystem forstudying human neurogenesis and offers a valuable platform forscreening the effect of drugs and bioactive compounds on human neural differentiation.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds",
pages = "113-113",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2151"
}

Pejić, J., Mojsin, M., Stojkovska, J., Medić, A., Petrović, I., Stevanović, M., Obradović, B.,& Milivojević, M.. (2023). Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 113-113.
https://hdl.handle.net/21.15107/rcub_imagine_2151

Pejić J, Mojsin M, Stojkovska J, Medić A, Petrović I, Stevanović M, Obradović B, Milivojević M. Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:113-113.
https://hdl.handle.net/21.15107/rcub_imagine_2151 .

Pejić, Jelena, Mojsin, Marija, Stojkovska, Jasmina, Medić, Aleksandra, Petrović, Isidora, Stevanović, Milena, Obradović, Bojana, Milivojević, Milena, "Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):113-113,
https://hdl.handle.net/21.15107/rcub_imagine_2151 .

TY  - JOUR
AU  - Radonjić, Mia
AU  - Petrović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1594
AB  - A multidisciplinary approach based on experiments and mathematical modeling was used in biomimetic system development for three-dimensional (3D) cultures of cancer cells. Specifically, two cancer cell lines, human embryonic teratocarcinoma NT2/D1 and rat glioma C6, were immobilized in alginate microbeads and microfibers, respectively, and cultured under static and flow conditions in perfusion bioreactors. At the same time, chemical engineering methods were applied to explain the obtained results. The superficial medium velocity of 80 mu m s(-1) induced lower viability of NT2/D1 cells in superficial microbead zones, implying adverse effects of fluid shear stresses estimated as similar to 67 mPa. On the contrary, similar velocity (100 mu m s(-1)) enhanced the proliferation of C6 glioma cells within microfibers compared to static controls. An additional study of silver release from nanocomposite Ag/honey/alginate microfibers under perfusion indicated that the medium partially flows through the hydrogel (interstitial velocity of similar to 10 nm s(-1)). Thus, a diffusion-advection-reaction model described the mass transport to immobilized cells within microfibers. Substances with diffusion coefficients of similar to 10(-)(19)-10(-11) m(2) s(-)(1) are sufficiently supplied by diffusion only, while those with significantly lower diffusivities (similar to 10(-1)(9) m(2) s(-1)) require additional convective transport. The present study demonstrates the selection and contribution of chemical engineering methods in tumor model system development.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations
EP  - 223
IS  - 3
SP  - 211
VL  - 28
DO  - 10.2298/CICEQ210607033R
ER  - 

@article{
author = "Radonjić, Mia and Petrović, Jelena and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2022",
abstract = "A multidisciplinary approach based on experiments and mathematical modeling was used in biomimetic system development for three-dimensional (3D) cultures of cancer cells. Specifically, two cancer cell lines, human embryonic teratocarcinoma NT2/D1 and rat glioma C6, were immobilized in alginate microbeads and microfibers, respectively, and cultured under static and flow conditions in perfusion bioreactors. At the same time, chemical engineering methods were applied to explain the obtained results. The superficial medium velocity of 80 mu m s(-1) induced lower viability of NT2/D1 cells in superficial microbead zones, implying adverse effects of fluid shear stresses estimated as similar to 67 mPa. On the contrary, similar velocity (100 mu m s(-1)) enhanced the proliferation of C6 glioma cells within microfibers compared to static controls. An additional study of silver release from nanocomposite Ag/honey/alginate microfibers under perfusion indicated that the medium partially flows through the hydrogel (interstitial velocity of similar to 10 nm s(-1)). Thus, a diffusion-advection-reaction model described the mass transport to immobilized cells within microfibers. Substances with diffusion coefficients of similar to 10(-)(19)-10(-11) m(2) s(-)(1) are sufficiently supplied by diffusion only, while those with significantly lower diffusivities (similar to 10(-1)(9) m(2) s(-1)) require additional convective transport. The present study demonstrates the selection and contribution of chemical engineering methods in tumor model system development.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations",
pages = "223-211",
number = "3",
volume = "28",
doi = "10.2298/CICEQ210607033R"
}

Radonjić, M., Petrović, J., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2022). Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations. in Chemical Industry & Chemical Engineering Quarterly
Savez hemijskih inženjera, Beograd., 28(3), 211-223.
https://doi.org/10.2298/CICEQ210607033R

Radonjić M, Petrović J, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations. in Chemical Industry & Chemical Engineering Quarterly. 2022;28(3):211-223.
doi:10.2298/CICEQ210607033R .

Radonjić, Mia, Petrović, Jelena, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations" in Chemical Industry & Chemical Engineering Quarterly, 28, no. 3 (2022):211-223,
https://doi.org/10.2298/CICEQ210607033R . .

TY  - CONF
AU  - Radonjić, Mia
AU  - Petrović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1586
PB  - Mary Ann Liebert, Inc, New Rochelle
C3  - Tissue Engineering Part A
T1  - Optimization of 3d cancer cell culture conditions by application of chemical engineering principles
EP  - S395
SP  - S395
VL  - 28
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1586
ER  - 

@conference{
author = "Radonjić, Mia and Petrović, Jelena and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2022",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Tissue Engineering Part A",
title = "Optimization of 3d cancer cell culture conditions by application of chemical engineering principles",
pages = "S395-S395",
volume = "28",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1586"
}

Radonjić, M., Petrović, J., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2022). Optimization of 3d cancer cell culture conditions by application of chemical engineering principles. in Tissue Engineering Part A
Mary Ann Liebert, Inc, New Rochelle., 28, S395-S395.
https://hdl.handle.net/21.15107/rcub_imagine_1586

Radonjić M, Petrović J, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Optimization of 3d cancer cell culture conditions by application of chemical engineering principles. in Tissue Engineering Part A. 2022;28:S395-S395.
https://hdl.handle.net/21.15107/rcub_imagine_1586 .

Radonjić, Mia, Petrović, Jelena, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Optimization of 3d cancer cell culture conditions by application of chemical engineering principles" in Tissue Engineering Part A, 28 (2022):S395-S395,
https://hdl.handle.net/21.15107/rcub_imagine_1586 .

TY  - JOUR
AU  - Stojkovska, Jasmina
AU  - Zvicer, Jovana
AU  - Milivojević, Milena
AU  - Petrović, Isidora
AU  - Stevanović, Milena
AU  - Obradović, Bojana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1363
AB  - Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska Industrija
T1  - Validation of a novel perfusion bioreactor system in cancer research
EP  - 196
IS  - 3
SP  - 187
VL  - 74
DO  - 10.2298/HEMIND200329015S
ER  - 

@article{
author = "Stojkovska, Jasmina and Zvicer, Jovana and Milivojević, Milena and Petrović, Isidora and Stevanović, Milena and Obradović, Bojana",
year = "2020",
abstract = "Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska Industrija",
title = "Validation of a novel perfusion bioreactor system in cancer research",
pages = "196-187",
number = "3",
volume = "74",
doi = "10.2298/HEMIND200329015S"
}

Stojkovska, J., Zvicer, J., Milivojević, M., Petrović, I., Stevanović, M.,& Obradović, B.. (2020). Validation of a novel perfusion bioreactor system in cancer research. in Hemijska Industrija
Savez hemijskih inženjera, Beograd., 74(3), 187-196.
https://doi.org/10.2298/HEMIND200329015S

Stojkovska J, Zvicer J, Milivojević M, Petrović I, Stevanović M, Obradović B. Validation of a novel perfusion bioreactor system in cancer research. in Hemijska Industrija. 2020;74(3):187-196.
doi:10.2298/HEMIND200329015S .

Stojkovska, Jasmina, Zvicer, Jovana, Milivojević, Milena, Petrović, Isidora, Stevanović, Milena, Obradović, Bojana, "Validation of a novel perfusion bioreactor system in cancer research" in Hemijska Industrija, 74, no. 3 (2020):187-196,
https://doi.org/10.2298/HEMIND200329015S . .