TY  - JOUR
AU  - Pekmezović, Marina
AU  - Aleksić, Ivana
AU  - Barac, Aleksandra
AU  - Arsić-Arsenijević, Valentina
AU  - Vasiljević, Branka
AU  - Nikodinović-Runić, Jasmina
AU  - Šenerović, Lidija
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/953
AB  - Mixed microbial infections caused by Pseudomonas aeruginosa and pathogenic fungi are commonly found in patients with chronic infections and constitute a significant health care burden. The aim of this study was to address the potential polymicrobial antibiofilm activity of pompia and grapefruit essential oils (EOs). The mechanism of antimicrobial activity of EOs was analysed. EOs of pompia and grapefruit inhibited fungal growth with MIC concentrations between 50 and 250 mg L-1, whereas no effect on P. aeruginosa growth was observed. Both citrus EOs inhibited formation of bacterial and fungal monomicrobial biofilms in concentrations of 50 mg L-1 and were efficient in potentiating the activity of clinically used antimicrobials in vitro. The concentration of 10 mg L-1 EOs inhibited mixed biofilm formation composed of P. aeruginosa and Aspergillus fumigatus or Scedosporium apiospermum. Citrus EOs affected quorum sensing in P. aeruginosa and caused fast permeabilisation of Candida albicans membrane. Pompia and grapefruit EOs potently inhibited biofilm formation and could be used for the control of common polymicrobial infections.
PB  - Oxford Univ Press, Oxford
T2  - Pathogens and Disease
T1  - Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species
IS  - 8
VL  - 74
DO  - 10.1093/femspd/ftw102
ER  - 

@article{
author = "Pekmezović, Marina and Aleksić, Ivana and Barac, Aleksandra and Arsić-Arsenijević, Valentina and Vasiljević, Branka and Nikodinović-Runić, Jasmina and Šenerović, Lidija",
year = "2016",
abstract = "Mixed microbial infections caused by Pseudomonas aeruginosa and pathogenic fungi are commonly found in patients with chronic infections and constitute a significant health care burden. The aim of this study was to address the potential polymicrobial antibiofilm activity of pompia and grapefruit essential oils (EOs). The mechanism of antimicrobial activity of EOs was analysed. EOs of pompia and grapefruit inhibited fungal growth with MIC concentrations between 50 and 250 mg L-1, whereas no effect on P. aeruginosa growth was observed. Both citrus EOs inhibited formation of bacterial and fungal monomicrobial biofilms in concentrations of 50 mg L-1 and were efficient in potentiating the activity of clinically used antimicrobials in vitro. The concentration of 10 mg L-1 EOs inhibited mixed biofilm formation composed of P. aeruginosa and Aspergillus fumigatus or Scedosporium apiospermum. Citrus EOs affected quorum sensing in P. aeruginosa and caused fast permeabilisation of Candida albicans membrane. Pompia and grapefruit EOs potently inhibited biofilm formation and could be used for the control of common polymicrobial infections.",
publisher = "Oxford Univ Press, Oxford",
journal = "Pathogens and Disease",
title = "Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species",
number = "8",
volume = "74",
doi = "10.1093/femspd/ftw102"
}

Pekmezović, M., Aleksić, I., Barac, A., Arsić-Arsenijević, V., Vasiljević, B., Nikodinović-Runić, J.,& Šenerović, L.. (2016). Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species. in Pathogens and Disease
Oxford Univ Press, Oxford., 74(8).
https://doi.org/10.1093/femspd/ftw102

Pekmezović M, Aleksić I, Barac A, Arsić-Arsenijević V, Vasiljević B, Nikodinović-Runić J, Šenerović L. Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species. in Pathogens and Disease. 2016;74(8).
doi:10.1093/femspd/ftw102 .

Pekmezović, Marina, Aleksić, Ivana, Barac, Aleksandra, Arsić-Arsenijević, Valentina, Vasiljević, Branka, Nikodinović-Runić, Jasmina, Šenerović, Lidija, "Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species" in Pathogens and Disease, 74, no. 8 (2016),
https://doi.org/10.1093/femspd/ftw102 . .

TY  - JOUR
AU  - Pekmezović, M.
AU  - Rajković, K.
AU  - Barac, A.
AU  - Šenerović, Lidija
AU  - Arsić-Arsenijević, Valentina
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/809
AB  - The antifungal effect of essential oils (EOs) of Thymus vulgaris L. (EOT.vulgaris) and Cinnamomum cassia L. (EOC.cassia) against Aspergillus flavus spores was evaluated by determining minimum inhibitory concentration, minimum fungicidal concentrations and fungicidal kinetics. Kinetic model of fungicidal activity of individual EOT.vulgaris and EOC.cassia was developed and its parameters were used to make EO mixture with optimal ratio of individual EOs. Synergism and speed of fungicidal effect of EO mixtures against A. flavus spores were evaluated. Kinetic model revealed optimal ratio EOT.vulgaris: EOC.cassia as 14:1 in mixture. Observed result was validated by comparing fungicidal effect of this mixture to commonly used ratio 1:1 and intermediate 7:1 EO mixtures. All three mixtures showed partial fungicidal synergism, but the time point of fungicidal effect was different. The fastest fungicidal effect was observed in 14:1 EO mixture (after 90 mm), followed by 7:1 (110 min) and 1:1 (180 min). The difference in the speed of fungicidal effect could not be detected using standard microdilution susceptibility tests, which demonstrated the importance and usefulness of developed kinetic model for further investigations.
PB  - Elsevier Science Bv, Amsterdam
T2  - Biochemical Engineering Journal
T1  - Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect
EP  - 137
SP  - 131
VL  - 99
DO  - 10.1016/j.bej.2015.03.024
ER  - 

@article{
author = "Pekmezović, M. and Rajković, K. and Barac, A. and Šenerović, Lidija and Arsić-Arsenijević, Valentina",
year = "2015",
abstract = "The antifungal effect of essential oils (EOs) of Thymus vulgaris L. (EOT.vulgaris) and Cinnamomum cassia L. (EOC.cassia) against Aspergillus flavus spores was evaluated by determining minimum inhibitory concentration, minimum fungicidal concentrations and fungicidal kinetics. Kinetic model of fungicidal activity of individual EOT.vulgaris and EOC.cassia was developed and its parameters were used to make EO mixture with optimal ratio of individual EOs. Synergism and speed of fungicidal effect of EO mixtures against A. flavus spores were evaluated. Kinetic model revealed optimal ratio EOT.vulgaris: EOC.cassia as 14:1 in mixture. Observed result was validated by comparing fungicidal effect of this mixture to commonly used ratio 1:1 and intermediate 7:1 EO mixtures. All three mixtures showed partial fungicidal synergism, but the time point of fungicidal effect was different. The fastest fungicidal effect was observed in 14:1 EO mixture (after 90 mm), followed by 7:1 (110 min) and 1:1 (180 min). The difference in the speed of fungicidal effect could not be detected using standard microdilution susceptibility tests, which demonstrated the importance and usefulness of developed kinetic model for further investigations.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Biochemical Engineering Journal",
title = "Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect",
pages = "137-131",
volume = "99",
doi = "10.1016/j.bej.2015.03.024"
}

Pekmezović, M., Rajković, K., Barac, A., Šenerović, L.,& Arsić-Arsenijević, V.. (2015). Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect. in Biochemical Engineering Journal
Elsevier Science Bv, Amsterdam., 99, 131-137.
https://doi.org/10.1016/j.bej.2015.03.024

Pekmezović M, Rajković K, Barac A, Šenerović L, Arsić-Arsenijević V. Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect. in Biochemical Engineering Journal. 2015;99:131-137.
doi:10.1016/j.bej.2015.03.024 .

Pekmezović, M., Rajković, K., Barac, A., Šenerović, Lidija, Arsić-Arsenijević, Valentina, "Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect" in Biochemical Engineering Journal, 99 (2015):131-137,
https://doi.org/10.1016/j.bej.2015.03.024 . .

TY  - JOUR
AU  - Rajković, Katarina
AU  - Pekmezović, Marina
AU  - Barac, Aleksandra
AU  - Nikodinović-Runić, Jasmina
AU  - Arsić-Arsenijević, Valentina
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/859
AB  - Antifungal effect of individual thyme (Thymus vulgaris L) and cinnamon (Cinnamomum cassia L.) essential oils (EOs) and mixture of thereof on Aspergillus flavus spores was investigated. In order to optimize the process variables (time of action, concentration of individual or mixture EOs and their mass ratio) for the antifungal effect of EO mixture, two models were developed: the response surface methodology (RSM) and artificial neural network (ANN) combined with genetic algorithm (GA). In RSM model, three factors were involved in Box-Behnken design that was applied for the experiment. Based on the mean relative percent deviation (MRPD), both models provided a good quality prediction for the antifungal effect in terms of all three process variables. RSM and ANN-GA techniques predicted the 0.5% as an optimum percentage concentration of EO mixture in EOs mass ratio T. vulgaris:C. cassia 1:1, ensuring the highest antifungal effect of 95.8% and 96.4% after 65 min. Both models were found useful for the optimization of the antifungal effect in vitro. ANN-GA was found more accurate in comparison to RSM due to its lower value of MRPD. Therefore, ANN-GA can be generally used for optimization and prediction of antimicrobial effects of 605 and their mixtures.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Inhibitory effect of thyme and cinnamon essential oils on Aspergillus flavus: Optimization and activity prediction model development
EP  - 13
SP  - 7
VL  - 65
DO  - 10.1016/j.indcrop.2014.11.039
ER  - 

@article{
author = "Rajković, Katarina and Pekmezović, Marina and Barac, Aleksandra and Nikodinović-Runić, Jasmina and Arsić-Arsenijević, Valentina",
year = "2015",
abstract = "Antifungal effect of individual thyme (Thymus vulgaris L) and cinnamon (Cinnamomum cassia L.) essential oils (EOs) and mixture of thereof on Aspergillus flavus spores was investigated. In order to optimize the process variables (time of action, concentration of individual or mixture EOs and their mass ratio) for the antifungal effect of EO mixture, two models were developed: the response surface methodology (RSM) and artificial neural network (ANN) combined with genetic algorithm (GA). In RSM model, three factors were involved in Box-Behnken design that was applied for the experiment. Based on the mean relative percent deviation (MRPD), both models provided a good quality prediction for the antifungal effect in terms of all three process variables. RSM and ANN-GA techniques predicted the 0.5% as an optimum percentage concentration of EO mixture in EOs mass ratio T. vulgaris:C. cassia 1:1, ensuring the highest antifungal effect of 95.8% and 96.4% after 65 min. Both models were found useful for the optimization of the antifungal effect in vitro. ANN-GA was found more accurate in comparison to RSM due to its lower value of MRPD. Therefore, ANN-GA can be generally used for optimization and prediction of antimicrobial effects of 605 and their mixtures.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Inhibitory effect of thyme and cinnamon essential oils on Aspergillus flavus: Optimization and activity prediction model development",
pages = "13-7",
volume = "65",
doi = "10.1016/j.indcrop.2014.11.039"
}

Rajković, K., Pekmezović, M., Barac, A., Nikodinović-Runić, J.,& Arsić-Arsenijević, V.. (2015). Inhibitory effect of thyme and cinnamon essential oils on Aspergillus flavus: Optimization and activity prediction model development. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam., 65, 7-13.
https://doi.org/10.1016/j.indcrop.2014.11.039

Rajković K, Pekmezović M, Barac A, Nikodinović-Runić J, Arsić-Arsenijević V. Inhibitory effect of thyme and cinnamon essential oils on Aspergillus flavus: Optimization and activity prediction model development. in Industrial Crops and Products. 2015;65:7-13.
doi:10.1016/j.indcrop.2014.11.039 .

Rajković, Katarina, Pekmezović, Marina, Barac, Aleksandra, Nikodinović-Runić, Jasmina, Arsić-Arsenijević, Valentina, "Inhibitory effect of thyme and cinnamon essential oils on Aspergillus flavus: Optimization and activity prediction model development" in Industrial Crops and Products, 65 (2015):7-13,
https://doi.org/10.1016/j.indcrop.2014.11.039 . .