TY  - CONF
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Morina, Filis
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović Jovanović, Sonja
PY  - 2023
UR  - https://thessinppo2023.com/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1850
AB  - Ramonda serbica Panc. is a resurrection plant that can survive long periods of desiccation and
fully restores its metabolic functions just one day after watering. The aim of this study was to
identify key candidates and metabolic pathways involved in R. serbica desiccation tolerance.
We combined differential transcriptomics and proteomics with the analysis of phenolics,
sugars, cell wall polymers and photosynthetic electron transport (PET) chain. TMT-based
proteomic analysis allowed the relative quantification of 1192 different protein groups, 408 of
which were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost
all differentially abundant proteins and transcripts related to photosynthetic processes were
downregulated in DL. Chlorophyll fluorescence measurements showed a shift from linear PET

to cyclic electron transport (CET). The levels of H2O2-scavenging enzymes, ascorbate-
glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase

(SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ZnSOD
isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs;
mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation led to cell wall
remodelling related to GLP-derived H2O2/HO●

activity and pectin demethylesterification. This
comprehensive study contributes to understanding the role and regulation of important
metabolic pathways during desiccation with the final aim to help improving the drought
tolerance in crops.
PB  - International Plant Proteomics Organization (INPPO)
C3  - 5th International Conference on Plant Proteomics
T1  - Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation  tolerance in Ramonda serbica Panc.
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1850
ER  - 

@conference{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja",
year = "2023",
abstract = "Ramonda serbica Panc. is a resurrection plant that can survive long periods of desiccation and
fully restores its metabolic functions just one day after watering. The aim of this study was to
identify key candidates and metabolic pathways involved in R. serbica desiccation tolerance.
We combined differential transcriptomics and proteomics with the analysis of phenolics,
sugars, cell wall polymers and photosynthetic electron transport (PET) chain. TMT-based
proteomic analysis allowed the relative quantification of 1192 different protein groups, 408 of
which were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost
all differentially abundant proteins and transcripts related to photosynthetic processes were
downregulated in DL. Chlorophyll fluorescence measurements showed a shift from linear PET

to cyclic electron transport (CET). The levels of H2O2-scavenging enzymes, ascorbate-
glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase

(SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ZnSOD
isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs;
mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation led to cell wall
remodelling related to GLP-derived H2O2/HO●

activity and pectin demethylesterification. This
comprehensive study contributes to understanding the role and regulation of important
metabolic pathways during desiccation with the final aim to help improving the drought
tolerance in crops.",
publisher = "International Plant Proteomics Organization (INPPO)",
journal = "5th International Conference on Plant Proteomics",
title = "Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation  tolerance in Ramonda serbica Panc.",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1850"
}

Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2023). Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation  tolerance in Ramonda serbica Panc.. in 5th International Conference on Plant Proteomics
International Plant Proteomics Organization (INPPO)..
https://hdl.handle.net/21.15107/rcub_imagine_1850

Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović Jovanović S. Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation  tolerance in Ramonda serbica Panc.. in 5th International Conference on Plant Proteomics. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_1850 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Integrative transcriptomic and TMT-based proteomic analysis reveals the desiccation  tolerance in Ramonda serbica Panc." in 5th International Conference on Plant Proteomics (2023),
https://hdl.handle.net/21.15107/rcub_imagine_1850 .

TY  - CONF
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Milić, Dejana
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović Jovanović, Sonja
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2005
AB  - The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully
recovers metabolic functions within one day upon watering. We aimed to identify key
candidates and pathways involved in desiccation tolerance in R. serbica by employing a
systems biology approach, combining transcriptomics and proteomics.
A total of 68,694 differentially expressed genes (DEGs; p-value<0.005 and abs(log2FC)≥2) were
obtained in R. serbica leaves upon desiccation. Among them, 23,935 and 26,169 genes were
upregulated and downregulated in desiccated leaves (DL) and hydrated leaves (HL), respectively.
By differential TMT-based proteomic analysis 1192 different protein groups were identified
after filtering with at least two unique peptides per protein. In total, 229 protein groups
were more abundant in HL and 179 in DL (p-value<0.05 and abs(FC)≥1.3). The majority
of the DAPs and DEGs involved in photosynthesis, transport, secondary metabolism, and
signaling, were less abundant in DL. On the other hand, proteins and transcripts associated
with fermentation, N-metabolism, heme, protein synthesis, folding and assembly, C1-
metabolism, and late embryogenesis abundant proteins, were more accumulated in DL.
A poor correlation between proteomic and transcriptomic results was detected for mitochondrial
electron transport and ATP production, gluconeogenesis, glycolysis, tricarboxylic
acid cycle, and enzymatic H2O2 scavengers due to different mRNA half-life, protein turnover,
dynamic posttranscriptional and posttranslational modifications. Finally, desiccation tolerance
in R. serbica is a species-specific process orchestrated by several metabolic pathways
that are temporally and compartmentally regulated at several levels.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Integration of differential transcriptomic and proteomic data in hydrated and desiccated leaves of Ramonda serbica Panc.
EP  - 65
SP  - 65
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2005
ER  - 

@conference{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Milić, Dejana and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja",
year = "2023",
abstract = "The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully
recovers metabolic functions within one day upon watering. We aimed to identify key
candidates and pathways involved in desiccation tolerance in R. serbica by employing a
systems biology approach, combining transcriptomics and proteomics.
A total of 68,694 differentially expressed genes (DEGs; p-value<0.005 and abs(log2FC)≥2) were
obtained in R. serbica leaves upon desiccation. Among them, 23,935 and 26,169 genes were
upregulated and downregulated in desiccated leaves (DL) and hydrated leaves (HL), respectively.
By differential TMT-based proteomic analysis 1192 different protein groups were identified
after filtering with at least two unique peptides per protein. In total, 229 protein groups
were more abundant in HL and 179 in DL (p-value<0.05 and abs(FC)≥1.3). The majority
of the DAPs and DEGs involved in photosynthesis, transport, secondary metabolism, and
signaling, were less abundant in DL. On the other hand, proteins and transcripts associated
with fermentation, N-metabolism, heme, protein synthesis, folding and assembly, C1-
metabolism, and late embryogenesis abundant proteins, were more accumulated in DL.
A poor correlation between proteomic and transcriptomic results was detected for mitochondrial
electron transport and ATP production, gluconeogenesis, glycolysis, tricarboxylic
acid cycle, and enzymatic H2O2 scavengers due to different mRNA half-life, protein turnover,
dynamic posttranscriptional and posttranslational modifications. Finally, desiccation tolerance
in R. serbica is a species-specific process orchestrated by several metabolic pathways
that are temporally and compartmentally regulated at several levels.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Integration of differential transcriptomic and proteomic data in hydrated and desiccated leaves of Ramonda serbica Panc.",
pages = "65-65",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2005"
}

Vidović, M., Battisti, I., Pantelić, A., Milić, D., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2023). Integration of differential transcriptomic and proteomic data in hydrated and desiccated leaves of Ramonda serbica Panc.. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 65-65.
https://hdl.handle.net/21.15107/rcub_imagine_2005

Vidović M, Battisti I, Pantelić A, Milić D, Arrigoni G, Masi A, Veljović Jovanović S. Integration of differential transcriptomic and proteomic data in hydrated and desiccated leaves of Ramonda serbica Panc.. in 4th Belgrade Bioinformatics Conference. 2023;4:65-65.
https://hdl.handle.net/21.15107/rcub_imagine_2005 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Milić, Dejana, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Integration of differential transcriptomic and proteomic data in hydrated and desiccated leaves of Ramonda serbica Panc." in 4th Belgrade Bioinformatics Conference, 4 (2023):65-65,
https://hdl.handle.net/21.15107/rcub_imagine_2005 .

TY  - CONF
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Morina, Filis
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović Jovanović, Sonja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1847
AB  - Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a long
period and fully resume metabolic functions upon rewatering in a very short period, even within
48 h. The goal of this study was to identify key candidates and pathways involved in desiccation
tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,
proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of the
cell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chain
were performed. In total, 1192 different protein groups were quantified by TMT-based comparative
quantitative proteomics. Among them, 408 protein groups showed a statistically significant
difference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, the
majority of proteins related to photosynthetic processes were less abundant, while chlorophyll
fluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclic
electron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione
cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) were
reduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins
(GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),
were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wall
polymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/
HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific
process orchestrated by several metabolic pathways and regulatory networks acting at the transcript,
protein, metabolite and physiological levels.
PB  - Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting
T1  - Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1847
ER  - 

@conference{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja",
year = "2022",
abstract = "Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a long
period and fully resume metabolic functions upon rewatering in a very short period, even within
48 h. The goal of this study was to identify key candidates and pathways involved in desiccation
tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,
proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of the
cell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chain
were performed. In total, 1192 different protein groups were quantified by TMT-based comparative
quantitative proteomics. Among them, 408 protein groups showed a statistically significant
difference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, the
majority of proteins related to photosynthetic processes were less abundant, while chlorophyll
fluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclic
electron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione
cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) were
reduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins
(GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),
were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wall
polymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/
HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific
process orchestrated by several metabolic pathways and regulatory networks acting at the transcript,
protein, metabolite and physiological levels.",
publisher = "Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting",
title = "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects",
pages = "57",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1847"
}

Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2022). Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology and 23rd SPPS Meeting
Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade., 57.
https://hdl.handle.net/21.15107/rcub_imagine_1847

Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović Jovanović S. Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology and 23rd SPPS Meeting. 2022;:57.
https://hdl.handle.net/21.15107/rcub_imagine_1847 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects" in 4th International Conference on Plant Biology and 23rd SPPS Meeting (2022):57,
https://hdl.handle.net/21.15107/rcub_imagine_1847 .

TY  - JOUR
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Morina, Filis
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović-Jovanović, Sonja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1604
AB  - The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully recovers metabolic functions within one day upon watering. This study aimed to identify key candidates and pathways involved in desiccation tolerance in R. serbica. We combined differential transcriptomics and proteomics, phenolic and sugar analysis, FTIR analysis of the cell wall polymers, and detailed analysis of the photosynthetic electron transport (PET) chain. The proteomic analysis allowed the relative quantification of 1192 different protein groups, of which 408 were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost all differentially abundant proteins related to photosynthetic processes were less abundant, while chlorophyll fluorescence measurements implied shifting from linear PET to cyclic electron transport (CET). The levels of H2O2 scavenging enzymes, ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase (SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ ZnSOD isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation provoked cell wall remodeling related to GLP-derived H2O2/HO center dot activity and pectin demethylesterification. This comprehensive study contributes to understanding the role and regulation of the main metabolic pathways during desiccation aiming at crop drought tolerance improvement.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study
IS  - 9
VL  - 11
DO  - 10.3390/plants11091199
ER  - 

@article{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović-Jovanović, Sonja",
year = "2022",
abstract = "The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully recovers metabolic functions within one day upon watering. This study aimed to identify key candidates and pathways involved in desiccation tolerance in R. serbica. We combined differential transcriptomics and proteomics, phenolic and sugar analysis, FTIR analysis of the cell wall polymers, and detailed analysis of the photosynthetic electron transport (PET) chain. The proteomic analysis allowed the relative quantification of 1192 different protein groups, of which 408 were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost all differentially abundant proteins related to photosynthetic processes were less abundant, while chlorophyll fluorescence measurements implied shifting from linear PET to cyclic electron transport (CET). The levels of H2O2 scavenging enzymes, ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase (SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ ZnSOD isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation provoked cell wall remodeling related to GLP-derived H2O2/HO center dot activity and pectin demethylesterification. This comprehensive study contributes to understanding the role and regulation of the main metabolic pathways during desiccation aiming at crop drought tolerance improvement.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study",
number = "9",
volume = "11",
doi = "10.3390/plants11091199"
}

Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović-Jovanović, S.. (2022). Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study. in Plants-Basel
MDPI, Basel., 11(9).
https://doi.org/10.3390/plants11091199

Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović-Jovanović S. Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study. in Plants-Basel. 2022;11(9).
doi:10.3390/plants11091199 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović-Jovanović, Sonja, "Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study" in Plants-Basel, 11, no. 9 (2022),
https://doi.org/10.3390/plants11091199 . .

TY  - CONF
AU  - Vidović, Marija
AU  - Battisti, Ilaria
AU  - Pantelić, Ana
AU  - Morina, Filis
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović Jovanović, Sonja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1833
AB  - Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a longperiod and fully resume metabolic functions upon rewatering in a very short period, even within48 h. The goal of this study was to identify key candidates and pathways involved in desiccation tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of thecell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chainwere performed. In total, 1192 different protein groups were quantified by TMT-based comparative quantitative proteomics. Among them, 408 protein groups showed a statistically significantdifference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, themajority of proteins related to photosynthetic processes were less abundant, while chlorophyllfluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclicelectron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) werereduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins (GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wallpolymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific process orchestrated by several metabolic pathways and regulatory networks acting at the transcript, protein, metabolite and physiological levels.
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting
T1  - Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects
EP  - 27
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1833
ER  - 

@conference{
author = "Vidović, Marija and Battisti, Ilaria and Pantelić, Ana and Morina, Filis and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja",
year = "2022",
abstract = "Ramonda serbica Panc. is a resurrection plant species that can survive desiccation for a longperiod and fully resume metabolic functions upon rewatering in a very short period, even within48 h. The goal of this study was to identify key candidates and pathways involved in desiccation tolerance in R. serbica. To achieve this, systems biology approach combining transcriptomics,proteomics, and analysis of specific metabolites was employed. In addition, FTIR analysis of thecell wall polymers and a detailed analysis of the photosynthetic electron transport (PET) chainwere performed. In total, 1192 different protein groups were quantified by TMT-based comparative quantitative proteomics. Among them, 408 protein groups showed a statistically significantdifference in abundance between hydrated (HL) and desiccated leaves (DL). Upon desiccation, themajority of proteins related to photosynthetic processes were less abundant, while chlorophyllfluorescence measurements implied shifting from linear photosynthetic transport (PET) to cyclicelectron transport (CET). The amounts of H2O2 scavenging enzymes, including ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn- superoxide dismutase (SOD) werereduced in DL. However, four Cu/ZnSOD isoforms, three polyphenol oxidases, six germin-like proteins (GLPs), and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins),were desiccation-inducible. Desiccation-induced cell wall remodelling by changes in cell wallpolymer composition might be linked with pectin demethylesterification and GLP-derived H2O2/HO•. Our study demonstrated that desiccation tolerance in R. serbica is a complex, species-specific process orchestrated by several metabolic pathways and regulatory networks acting at the transcript, protein, metabolite and physiological levels.",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting",
title = "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1833"
}

Vidović, M., Battisti, I., Pantelić, A., Morina, F., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2022). Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 27-27.
https://hdl.handle.net/21.15107/rcub_imagine_1833

Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Veljović Jovanović S. Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects. in 4th International Conference on Plant Biology and 23rd SPPS Meeting. 2022;:27-27.
https://hdl.handle.net/21.15107/rcub_imagine_1833 .

Vidović, Marija, Battisti, Ilaria, Pantelić, Ana, Morina, Filis, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Mechanisms of desiccation tolerance in Ramonda serbica Panc.: transcriptomic, proteomic, metabolomic, and photosynthetic aspects" in 4th International Conference on Plant Biology and 23rd SPPS Meeting (2022):27-27,
https://hdl.handle.net/21.15107/rcub_imagine_1833 .

TY  - CONF
AU  - Vidović, Marija
AU  - Stevanović, Strahinja
AU  - Franchin, Cinzia
AU  - Battisti, Ilaria
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović Jovanović, Sonja
PY  - 2021
UR  - https://inppo.org/inppo2020/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1835
AB  - Resurrection plant Ramonda serbica Panc. survives desiccation for a long period and fully recovers metabolic functions already within one day upon watering [1]. Besides osmotic stress, desiccation provokes the accelerated generation of reactive oxygen species. The aim of our study was obtaining more insight into the mechanisms of desiccation tolerance in R. serbica by TMT labelled comparative quantitative proteomics of hydrated (HL) and desiccated leaves (DL). After de novo transcriptome analysis, 189456 transcripts with 189003 unigenes were annotated with seven common databases. Proteomic analysis allowed for the relative quantification of 895 different protein groups, 321 with a statistically significant difference in abundance between FL and DL. Among them, 25% referred to chloroplast and almost the same percentage were associated with desiccation and oxidative stress. Almost all differentially abundant proteins related to photosynthetic processes were down-regulated in DL, while those required for protein translation were more abundant in HL. Within differentially abundant proteins involved in antioxidative defence, the levels of enzymes involved in ascorbate-glutathione cycle, peroxiredoxins, Fe and Mn superoxide dismutase (SOD) were all reduced in DL, while germin-like proteins, three Cu/Zn SOD isoforms and polyphenol oxidases were more abundant in DL compared with HL. The protein family with the highest number of members showing the greatest accumulation upon desiccation comprised twenty different late embryogenesis abundant proteins (LEAPs), similarly as found by differential transcriptomic analysis. Taken together, our results imply a key role of LEAPs and Cu/Zn SOD in protective mechanism against desiccation in R. serbica, that may have significant implications on drought-related studies of crops grown in arid areas. This work was supported by the Science Fund of the Republic of Serbia (PROMIS project LEAPSyn-SCI, grant number 6039663). M.V. wishes to acknowledge the support of COST Action BM1405 for approving STSM in Padua during 2017 and 2018.
PB  - International Plant Proteomics Organization
C3  - The Fourth Conference of the International Plant Proteomics Organization
T1  - Twenty different late embryogenesis abundant proteins (LEAPs) accumulate in desiccated Ramonda serbica leaves
EP  - 44
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1835
ER  - 

@conference{
author = "Vidović, Marija and Stevanović, Strahinja and Franchin, Cinzia and Battisti, Ilaria and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja",
year = "2021",
abstract = "Resurrection plant Ramonda serbica Panc. survives desiccation for a long period and fully recovers metabolic functions already within one day upon watering [1]. Besides osmotic stress, desiccation provokes the accelerated generation of reactive oxygen species. The aim of our study was obtaining more insight into the mechanisms of desiccation tolerance in R. serbica by TMT labelled comparative quantitative proteomics of hydrated (HL) and desiccated leaves (DL). After de novo transcriptome analysis, 189456 transcripts with 189003 unigenes were annotated with seven common databases. Proteomic analysis allowed for the relative quantification of 895 different protein groups, 321 with a statistically significant difference in abundance between FL and DL. Among them, 25% referred to chloroplast and almost the same percentage were associated with desiccation and oxidative stress. Almost all differentially abundant proteins related to photosynthetic processes were down-regulated in DL, while those required for protein translation were more abundant in HL. Within differentially abundant proteins involved in antioxidative defence, the levels of enzymes involved in ascorbate-glutathione cycle, peroxiredoxins, Fe and Mn superoxide dismutase (SOD) were all reduced in DL, while germin-like proteins, three Cu/Zn SOD isoforms and polyphenol oxidases were more abundant in DL compared with HL. The protein family with the highest number of members showing the greatest accumulation upon desiccation comprised twenty different late embryogenesis abundant proteins (LEAPs), similarly as found by differential transcriptomic analysis. Taken together, our results imply a key role of LEAPs and Cu/Zn SOD in protective mechanism against desiccation in R. serbica, that may have significant implications on drought-related studies of crops grown in arid areas. This work was supported by the Science Fund of the Republic of Serbia (PROMIS project LEAPSyn-SCI, grant number 6039663). M.V. wishes to acknowledge the support of COST Action BM1405 for approving STSM in Padua during 2017 and 2018.",
publisher = "International Plant Proteomics Organization",
journal = "The Fourth Conference of the International Plant Proteomics Organization",
title = "Twenty different late embryogenesis abundant proteins (LEAPs) accumulate in desiccated Ramonda serbica leaves",
pages = "44-43",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1835"
}

Vidović, M., Stevanović, S., Franchin, C., Battisti, I., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2021). Twenty different late embryogenesis abundant proteins (LEAPs) accumulate in desiccated Ramonda serbica leaves. in The Fourth Conference of the International Plant Proteomics Organization
International Plant Proteomics Organization., 43-44.
https://hdl.handle.net/21.15107/rcub_imagine_1835

Vidović M, Stevanović S, Franchin C, Battisti I, Arrigoni G, Masi A, Veljović Jovanović S. Twenty different late embryogenesis abundant proteins (LEAPs) accumulate in desiccated Ramonda serbica leaves. in The Fourth Conference of the International Plant Proteomics Organization. 2021;:43-44.
https://hdl.handle.net/21.15107/rcub_imagine_1835 .

Vidović, Marija, Stevanović, Strahinja, Franchin, Cinzia, Battisti, Ilaria, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Twenty different late embryogenesis abundant proteins (LEAPs) accumulate in desiccated Ramonda serbica leaves" in The Fourth Conference of the International Plant Proteomics Organization (2021):43-44,
https://hdl.handle.net/21.15107/rcub_imagine_1835 .

TY  - CONF
AU  - Stevanović, Strahinja
AU  - Franchin, Cinzia
AU  - Battisti, Ilaria
AU  - Arrigoni, Giorgio
AU  - Masi, Antonio
AU  - Veljović Jovanović, Sonja
PY  - 2021
UR  - https://inppo.org/inppo2020/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1857
AB  - Resurrection plant Ramonda serbica Panc. survives desiccation for a long period and fully recovers metabolic functions already within one day upon watering [1]. Besides osmotic stress, desiccation provokes the accelerated generation of reactive oxygen species. The aim of our study was obtaining more insight into the mechanisms of desiccation tolerance in R. serbica by TMT labelled comparative quantitative proteomics of hydrated (HL) and desiccated leaves (DL). After de novo transcriptome analysis, 189456 transcripts with 189003 unigenes were annotated with seven common databases. Proteomic analysis allowed for the relative quantification of 895 different protein groups, 321 with a statistically significant difference in abundance between FL and DL. Among them, 25% referred to chloroplast and almost the same percentage were associated with desiccation and oxidative stress. Almost all differentially abundant proteins related to photosynthetic processes were down-regulated in DL, while those required for protein translation were more abundant in HL. Within differentially abundant proteins involved in antioxidative defence, the levels of enzymes involved in ascorbate-glutathione cycle, peroxiredoxins, Fe and Mn superoxide dismutase (SOD) were all reduced in DL, while germin-like proteins, three Cu/Zn SOD isoforms and polyphenol oxidases were more abundant in DL compared with HL. The protein family with the highest number of members showing the greatest accumulation upon desiccation comprised twenty different late embryogenesis abundant proteins (LEAPs), similarly as found by differential transcriptomic analysis. Taken together, our results imply a key role of LEAPs and Cu/Zn SOD in protective mechanism against desiccation in R. serbica, that may have significant implications on drought-related studies of crops grown in arid areas. This work was supported by the Science Fund of the Republic of Serbia (PROMIS project LEAPSyn-SCI, grant number 6039663). M.V. wishes to acknowledge the support of COST Action BM1405 for approving STSM in Padua during 2017 and 2018.
PB  - International Plant Proteomics Organization
C3  - The Fourth Conference of the International Plant Proteomics Organization
T1  - Twenty different late embryogenesis abundant proteins (LEAPs) accumulate in desiccated Ramonda serbica leaves
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1857
ER  - 

@conference{
author = "Stevanović, Strahinja and Franchin, Cinzia and Battisti, Ilaria and Arrigoni, Giorgio and Masi, Antonio and Veljović Jovanović, Sonja",
year = "2021",
abstract = "Resurrection plant Ramonda serbica Panc. survives desiccation for a long period and fully recovers metabolic functions already within one day upon watering [1]. Besides osmotic stress, desiccation provokes the accelerated generation of reactive oxygen species. The aim of our study was obtaining more insight into the mechanisms of desiccation tolerance in R. serbica by TMT labelled comparative quantitative proteomics of hydrated (HL) and desiccated leaves (DL). After de novo transcriptome analysis, 189456 transcripts with 189003 unigenes were annotated with seven common databases. Proteomic analysis allowed for the relative quantification of 895 different protein groups, 321 with a statistically significant difference in abundance between FL and DL. Among them, 25% referred to chloroplast and almost the same percentage were associated with desiccation and oxidative stress. Almost all differentially abundant proteins related to photosynthetic processes were down-regulated in DL, while those required for protein translation were more abundant in HL. Within differentially abundant proteins involved in antioxidative defence, the levels of enzymes involved in ascorbate-glutathione cycle, peroxiredoxins, Fe and Mn superoxide dismutase (SOD) were all reduced in DL, while germin-like proteins, three Cu/Zn SOD isoforms and polyphenol oxidases were more abundant in DL compared with HL. The protein family with the highest number of members showing the greatest accumulation upon desiccation comprised twenty different late embryogenesis abundant proteins (LEAPs), similarly as found by differential transcriptomic analysis. Taken together, our results imply a key role of LEAPs and Cu/Zn SOD in protective mechanism against desiccation in R. serbica, that may have significant implications on drought-related studies of crops grown in arid areas. This work was supported by the Science Fund of the Republic of Serbia (PROMIS project LEAPSyn-SCI, grant number 6039663). M.V. wishes to acknowledge the support of COST Action BM1405 for approving STSM in Padua during 2017 and 2018.",
publisher = "International Plant Proteomics Organization",
journal = "The Fourth Conference of the International Plant Proteomics Organization",
title = "Twenty different late embryogenesis abundant proteins (LEAPs) accumulate in desiccated Ramonda serbica leaves",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1857"
}

Stevanović, S., Franchin, C., Battisti, I., Arrigoni, G., Masi, A.,& Veljović Jovanović, S.. (2021). Twenty different late embryogenesis abundant proteins (LEAPs) accumulate in desiccated Ramonda serbica leaves. in The Fourth Conference of the International Plant Proteomics Organization
International Plant Proteomics Organization..
https://hdl.handle.net/21.15107/rcub_imagine_1857

Stevanović S, Franchin C, Battisti I, Arrigoni G, Masi A, Veljović Jovanović S. Twenty different late embryogenesis abundant proteins (LEAPs) accumulate in desiccated Ramonda serbica leaves. in The Fourth Conference of the International Plant Proteomics Organization. 2021;.
https://hdl.handle.net/21.15107/rcub_imagine_1857 .

Stevanović, Strahinja, Franchin, Cinzia, Battisti, Ilaria, Arrigoni, Giorgio, Masi, Antonio, Veljović Jovanović, Sonja, "Twenty different late embryogenesis abundant proteins (LEAPs) accumulate in desiccated Ramonda serbica leaves" in The Fourth Conference of the International Plant Proteomics Organization (2021),
https://hdl.handle.net/21.15107/rcub_imagine_1857 .

TY  - DATA
AU  - Vidović, Marija
AU  - Franchin, Cinzia
AU  - Morina, Filis
AU  - Veljović-Jovanović, Sonja
AU  - Masi, Antonio
AU  - Arrigoni, Giorgio
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1837
PB  - SpringerLink
T2  - Analytical and Bioanalytical Chemistry
T1  - Supplementary data for the article: Vidović, M.; Franchin, C.; Morina, F.; Veljović-Jovanović, S.; Masi, A.; Arrigoni, G. Efficient Protein Extraction for Shotgun Proteomics from Hydrated and Desiccated Leaves of Resurrection Ramonda Serbica Plants. Anal Bioanal Chem 2020, 412 (30), 8299–8312. https://doi.org/10.1007/s00216-020-02965-2.
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1837
ER  - 

@misc{
author = "Vidović, Marija and Franchin, Cinzia and Morina, Filis and Veljović-Jovanović, Sonja and Masi, Antonio and Arrigoni, Giorgio",
year = "2020",
publisher = "SpringerLink",
journal = "Analytical and Bioanalytical Chemistry",
title = "Supplementary data for the article: Vidović, M.; Franchin, C.; Morina, F.; Veljović-Jovanović, S.; Masi, A.; Arrigoni, G. Efficient Protein Extraction for Shotgun Proteomics from Hydrated and Desiccated Leaves of Resurrection Ramonda Serbica Plants. Anal Bioanal Chem 2020, 412 (30), 8299–8312. https://doi.org/10.1007/s00216-020-02965-2.",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1837"
}

Vidović, M., Franchin, C., Morina, F., Veljović-Jovanović, S., Masi, A.,& Arrigoni, G.. (2020). Supplementary data for the article: Vidović, M.; Franchin, C.; Morina, F.; Veljović-Jovanović, S.; Masi, A.; Arrigoni, G. Efficient Protein Extraction for Shotgun Proteomics from Hydrated and Desiccated Leaves of Resurrection Ramonda Serbica Plants. Anal Bioanal Chem 2020, 412 (30), 8299–8312. https://doi.org/10.1007/s00216-020-02965-2.. in Analytical and Bioanalytical Chemistry
SpringerLink..
https://hdl.handle.net/21.15107/rcub_imagine_1837

Vidović M, Franchin C, Morina F, Veljović-Jovanović S, Masi A, Arrigoni G. Supplementary data for the article: Vidović, M.; Franchin, C.; Morina, F.; Veljović-Jovanović, S.; Masi, A.; Arrigoni, G. Efficient Protein Extraction for Shotgun Proteomics from Hydrated and Desiccated Leaves of Resurrection Ramonda Serbica Plants. Anal Bioanal Chem 2020, 412 (30), 8299–8312. https://doi.org/10.1007/s00216-020-02965-2.. in Analytical and Bioanalytical Chemistry. 2020;.
https://hdl.handle.net/21.15107/rcub_imagine_1837 .

Vidović, Marija, Franchin, Cinzia, Morina, Filis, Veljović-Jovanović, Sonja, Masi, Antonio, Arrigoni, Giorgio, "Supplementary data for the article: Vidović, M.; Franchin, C.; Morina, F.; Veljović-Jovanović, S.; Masi, A.; Arrigoni, G. Efficient Protein Extraction for Shotgun Proteomics from Hydrated and Desiccated Leaves of Resurrection Ramonda Serbica Plants. Anal Bioanal Chem 2020, 412 (30), 8299–8312. https://doi.org/10.1007/s00216-020-02965-2." in Analytical and Bioanalytical Chemistry (2020),
https://hdl.handle.net/21.15107/rcub_imagine_1837 .

TY  - JOUR
AU  - Vidović, Marija
AU  - Franchin, Cinzia
AU  - Morina, Filis
AU  - Veljović-Jovanović, Sonja
AU  - Masi, Antonio
AU  - Arrigoni, Giorgio
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1364
AB  - Resurrection plantRamonda serbicais a suitable model to investigate vegetative desiccation tolerance. However, the detailed study of these mechanisms at the protein level is hampered by the severe tissue water loss, high amount of phenolics and polysaccharide, and possible protein modifications and aggregations during the extraction and purification steps. When applied toR.serbicaleaves, widely used protein extraction protocols containing polyvinylpolypyrrolidone and ascorbate, as well as the phenol/SDS/buffer-based protocol recommended for recalcitrant plant tissues failed to eliminate persistent contamination and ensure high protein quality. Here we compared three protein extraction approaches aiming to establish the optimal one for both hydrated and desiccatedR.serbicaleaves. To evaluate the efficacy of these protocols by shotgun proteomics, we also created the firstR.serbicaannotated transcriptome database, available at. The detergent-free phenol-based extraction combined with dodecyl-beta-D-maltoside-assisted extraction enabled high-yield and high-purity protein extracts. The phenol-based protocol improved the protein-band resolution, band number, and intensity upon electrophoresis, and increased the protein yield and the number of identified peptides and protein groups by LC-MS/MS. Additionally, dodecyl-beta-D-maltoside enabled solubilisation and identification of more membrane-associated proteins. The presented study paves the way for investigating the desiccation tolerance inR.serbica, and we recommend this protocol for similar recalcitrant plant material.
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants
EP  - 8312
IS  - 30
SP  - 8299
VL  - 412
DO  - 10.1007/s00216-020-02965-2
ER  - 

@article{
author = "Vidović, Marija and Franchin, Cinzia and Morina, Filis and Veljović-Jovanović, Sonja and Masi, Antonio and Arrigoni, Giorgio",
year = "2020",
abstract = "Resurrection plantRamonda serbicais a suitable model to investigate vegetative desiccation tolerance. However, the detailed study of these mechanisms at the protein level is hampered by the severe tissue water loss, high amount of phenolics and polysaccharide, and possible protein modifications and aggregations during the extraction and purification steps. When applied toR.serbicaleaves, widely used protein extraction protocols containing polyvinylpolypyrrolidone and ascorbate, as well as the phenol/SDS/buffer-based protocol recommended for recalcitrant plant tissues failed to eliminate persistent contamination and ensure high protein quality. Here we compared three protein extraction approaches aiming to establish the optimal one for both hydrated and desiccatedR.serbicaleaves. To evaluate the efficacy of these protocols by shotgun proteomics, we also created the firstR.serbicaannotated transcriptome database, available at. The detergent-free phenol-based extraction combined with dodecyl-beta-D-maltoside-assisted extraction enabled high-yield and high-purity protein extracts. The phenol-based protocol improved the protein-band resolution, band number, and intensity upon electrophoresis, and increased the protein yield and the number of identified peptides and protein groups by LC-MS/MS. Additionally, dodecyl-beta-D-maltoside enabled solubilisation and identification of more membrane-associated proteins. The presented study paves the way for investigating the desiccation tolerance inR.serbica, and we recommend this protocol for similar recalcitrant plant material.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants",
pages = "8312-8299",
number = "30",
volume = "412",
doi = "10.1007/s00216-020-02965-2"
}

Vidović, M., Franchin, C., Morina, F., Veljović-Jovanović, S., Masi, A.,& Arrigoni, G.. (2020). Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg., 412(30), 8299-8312.
https://doi.org/10.1007/s00216-020-02965-2

Vidović M, Franchin C, Morina F, Veljović-Jovanović S, Masi A, Arrigoni G. Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants. in Analytical and Bioanalytical Chemistry. 2020;412(30):8299-8312.
doi:10.1007/s00216-020-02965-2 .

Vidović, Marija, Franchin, Cinzia, Morina, Filis, Veljović-Jovanović, Sonja, Masi, Antonio, Arrigoni, Giorgio, "Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants" in Analytical and Bioanalytical Chemistry, 412, no. 30 (2020):8299-8312,
https://doi.org/10.1007/s00216-020-02965-2 . .