TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Savić, Jelena
AU  - Jovanović, Živko
AU  - Miljuš-Đukić, Jovanka
AU  - Sencanski, Jelena
AU  - Simonović, Mladen
AU  - Radović, Svetlana
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1181
AB  - This study investigated the role of antioxidant system of Alyssum markgrafii, during long-term exposure to 0.5 or 1 mM NiCl2 x 6H(2)O in vitro. Applied methodology included sample preparation protocol which reduces oxidation of key metabolites along with novel luminescent method and well-established photometric procedures. During 5-week treatments, plants accumulated 1121 and 2470 ppm of Ni2+ respectively, followed by severe growth retardation, chlorophyll degradation and peroxidation of lipids. These effects were more pronounced after 1 mM Ni2+ treatment and additionally accompanied by increased water loss. Activities of luminol-converting peroxidases and glutathione reductase upon 0.5 mM treatment were increased while catalase and superoxide dismutase were diminished. The fact that these two groups of enzymes run in antiparallel might suggest functional redistribution between antioxidant enzymes rather than orchestrated action to prevent oxidative damage. Total antioxidant capacity (TAC) was also increased after 0.5 mM treatment which coincided with increased GR activity and elevated glutathione content indicating this low molecular weight antioxidant as an important factor associated with nickel tolerance. This study also emphasizes the possible important role of luminol-converting peroxidases in nickel hyperaccumulation, although they are not considered as antioxidant enzymes sensu stricto since some of them can also produce reactive oxygen species as well.
PB  - Springer Heidelberg, Heidelberg
T2  - Acta Physiologiae Plantarum
T1  - Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro
IS  - 6
VL  - 40
DO  - 10.1007/s11738-018-2677-9
ER  - 

@article{
author = "Stanisavljević, Nemanja and Savić, Jelena and Jovanović, Živko and Miljuš-Đukić, Jovanka and Sencanski, Jelena and Simonović, Mladen and Radović, Svetlana and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2018",
abstract = "This study investigated the role of antioxidant system of Alyssum markgrafii, during long-term exposure to 0.5 or 1 mM NiCl2 x 6H(2)O in vitro. Applied methodology included sample preparation protocol which reduces oxidation of key metabolites along with novel luminescent method and well-established photometric procedures. During 5-week treatments, plants accumulated 1121 and 2470 ppm of Ni2+ respectively, followed by severe growth retardation, chlorophyll degradation and peroxidation of lipids. These effects were more pronounced after 1 mM Ni2+ treatment and additionally accompanied by increased water loss. Activities of luminol-converting peroxidases and glutathione reductase upon 0.5 mM treatment were increased while catalase and superoxide dismutase were diminished. The fact that these two groups of enzymes run in antiparallel might suggest functional redistribution between antioxidant enzymes rather than orchestrated action to prevent oxidative damage. Total antioxidant capacity (TAC) was also increased after 0.5 mM treatment which coincided with increased GR activity and elevated glutathione content indicating this low molecular weight antioxidant as an important factor associated with nickel tolerance. This study also emphasizes the possible important role of luminol-converting peroxidases in nickel hyperaccumulation, although they are not considered as antioxidant enzymes sensu stricto since some of them can also produce reactive oxygen species as well.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Acta Physiologiae Plantarum",
title = "Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro",
number = "6",
volume = "40",
doi = "10.1007/s11738-018-2677-9"
}

Stanisavljević, N., Savić, J., Jovanović, Ž., Miljuš-Đukić, J., Sencanski, J., Simonović, M., Radović, S., Vinterhalter, D.,& Vinterhalter, B.. (2018). Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro. in Acta Physiologiae Plantarum
Springer Heidelberg, Heidelberg., 40(6).
https://doi.org/10.1007/s11738-018-2677-9

Stanisavljević N, Savić J, Jovanović Ž, Miljuš-Đukić J, Sencanski J, Simonović M, Radović S, Vinterhalter D, Vinterhalter B. Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro. in Acta Physiologiae Plantarum. 2018;40(6).
doi:10.1007/s11738-018-2677-9 .

Stanisavljević, Nemanja, Savić, Jelena, Jovanović, Živko, Miljuš-Đukić, Jovanka, Sencanski, Jelena, Simonović, Mladen, Radović, Svetlana, Vinterhalter, Dragan, Vinterhalter, Branka, "Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro" in Acta Physiologiae Plantarum, 40, no. 6 (2018),
https://doi.org/10.1007/s11738-018-2677-9 . .

TY  - JOUR
AU  - Mitić, Nevena
AU  - Stanisić, Mariana
AU  - Savić, Jelena
AU  - Cosić, Tatjana
AU  - Stanisavljević, Nemanja
AU  - Miljuš-Đukić, Jovanka
AU  - Marin, Marija
AU  - Radović, Svetlana
AU  - Ninković, Slavica
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1139
AB  - Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataa seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.
PB  - Springer Wien, Wien
T2  - Protoplasma
T1  - Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate
EP  - 1692
IS  - 6
SP  - 1683
VL  - 255
DO  - 10.1007/s00709-018-1250-0
ER  - 

@article{
author = "Mitić, Nevena and Stanisić, Mariana and Savić, Jelena and Cosić, Tatjana and Stanisavljević, Nemanja and Miljuš-Đukić, Jovanka and Marin, Marija and Radović, Svetlana and Ninković, Slavica",
year = "2018",
abstract = "Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataa seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.",
publisher = "Springer Wien, Wien",
journal = "Protoplasma",
title = "Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate",
pages = "1692-1683",
number = "6",
volume = "255",
doi = "10.1007/s00709-018-1250-0"
}

Mitić, N., Stanisić, M., Savić, J., Cosić, T., Stanisavljević, N., Miljuš-Đukić, J., Marin, M., Radović, S.,& Ninković, S.. (2018). Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate. in Protoplasma
Springer Wien, Wien., 255(6), 1683-1692.
https://doi.org/10.1007/s00709-018-1250-0

Mitić N, Stanisić M, Savić J, Cosić T, Stanisavljević N, Miljuš-Đukić J, Marin M, Radović S, Ninković S. Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate. in Protoplasma. 2018;255(6):1683-1692.
doi:10.1007/s00709-018-1250-0 .

Mitić, Nevena, Stanisić, Mariana, Savić, Jelena, Cosić, Tatjana, Stanisavljević, Nemanja, Miljuš-Đukić, Jovanka, Marin, Marija, Radović, Svetlana, Ninković, Slavica, "Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate" in Protoplasma, 255, no. 6 (2018):1683-1692,
https://doi.org/10.1007/s00709-018-1250-0 . .

TY  - JOUR
AU  - Nikolić, R.
AU  - Zdravkovic-Korac, S.
AU  - Ninković, S.
AU  - Dragicević, M.
AU  - Miljuš-Đukić, Jovanka
AU  - Banović Đeri, Bojana
AU  - Bohanec, B.
AU  - Savić, Jelena
AU  - Mitić, N.
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/630
AB  - Resistance to the non-selective herbicide dl-phosphinothricin (PPT) was introduced into commercial Lotus corniculatus cv. Bokor by co-cultivation of cotyledons with Agrobacterium tumefaciensAGL1 harbouring the binary vector pDM805 which contains the bialaphos resistance gene (bar) from Streptomyces hygroscopicus encoding phosphinothricin acetyltransferase (PAT) and the uidA gene encoding -glucuronidase. The half-cotyledon explants were precultured on regeneration Murashige and Skoog's (MS) medium supplemented with 6-benzyladenine (BA) and 1-naphthaleneacetic acid (NAA) at 0.5mgL(-1) each, 3days prior to infection. Upon co-cultivation, the explants were cultured on PPT-free regeneration medium for 10days, and then subcultured on regeneration/selection media with increasing PPT concentrations (5-7mgL(-1)) for about 18weeks. Out of 480 initially co-cultivated explants, 272 regenerated shoots survived the entire PPT selection procedure. Resistant shoots were grown further, multiplied by tillering that was additionally promoted by PPT and rooted on hormone-free MS medium containing 5mgL(-1) PPT. Established shoot cultures, continuously maintained on the same medium, have preserved PPT resistance up to now (more than 2years). Transformed plants assessed in vitro and in a greenhouse were tolerant to the herbicide PPT at 300mgL(-1) equivalent to more than twofold the recommended field dosage for weed eradication. Applied PPT treatment did not affect the activities of glutamine synthetase (GS; EC 6.3.1.2) and NADH-dependent glutamate dehydrogenase (NADH-GDH; EC 1.4.1.2) in transformed plants. However, PPT did increase the mobility of glutamine synthetase isoforms GS1 and GS2 as well as the inhibition of an additional high mobility GS (hmGS) activity. In untransformed plants, PPT treatment reduced total GS activity by 4.4-fold while contrary the activity of NADH-GDH was increased by ninefold. All transformed herbicide-resistant plants were phenotypically normal and exhibited genomic stability, as were the untransformed plants analysed by flow cytometry. Under greenhouse conditions, they grew to maturity, flowered and set seeds. Stable integration and expression of the bar gene in T0 and T1 plants were confirmed by Southern and Western blot analysis, while integration of the reporter uidA gene did not occur. The bar gene was inherited in a Mendelian fashion by the progeny, as detected by PPT resistance. The production of PPT-resistant plants may have significant practical applications in weed control in fields of L. corniculatus.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin
EP  - 493
IS  - 3
SP  - 475
VL  - 163
DO  - 10.1111/aab.12071
ER  - 

@article{
author = "Nikolić, R. and Zdravkovic-Korac, S. and Ninković, S. and Dragicević, M. and Miljuš-Đukić, Jovanka and Banović Đeri, Bojana and Bohanec, B. and Savić, Jelena and Mitić, N.",
year = "2013",
abstract = "Resistance to the non-selective herbicide dl-phosphinothricin (PPT) was introduced into commercial Lotus corniculatus cv. Bokor by co-cultivation of cotyledons with Agrobacterium tumefaciensAGL1 harbouring the binary vector pDM805 which contains the bialaphos resistance gene (bar) from Streptomyces hygroscopicus encoding phosphinothricin acetyltransferase (PAT) and the uidA gene encoding -glucuronidase. The half-cotyledon explants were precultured on regeneration Murashige and Skoog's (MS) medium supplemented with 6-benzyladenine (BA) and 1-naphthaleneacetic acid (NAA) at 0.5mgL(-1) each, 3days prior to infection. Upon co-cultivation, the explants were cultured on PPT-free regeneration medium for 10days, and then subcultured on regeneration/selection media with increasing PPT concentrations (5-7mgL(-1)) for about 18weeks. Out of 480 initially co-cultivated explants, 272 regenerated shoots survived the entire PPT selection procedure. Resistant shoots were grown further, multiplied by tillering that was additionally promoted by PPT and rooted on hormone-free MS medium containing 5mgL(-1) PPT. Established shoot cultures, continuously maintained on the same medium, have preserved PPT resistance up to now (more than 2years). Transformed plants assessed in vitro and in a greenhouse were tolerant to the herbicide PPT at 300mgL(-1) equivalent to more than twofold the recommended field dosage for weed eradication. Applied PPT treatment did not affect the activities of glutamine synthetase (GS; EC 6.3.1.2) and NADH-dependent glutamate dehydrogenase (NADH-GDH; EC 1.4.1.2) in transformed plants. However, PPT did increase the mobility of glutamine synthetase isoforms GS1 and GS2 as well as the inhibition of an additional high mobility GS (hmGS) activity. In untransformed plants, PPT treatment reduced total GS activity by 4.4-fold while contrary the activity of NADH-GDH was increased by ninefold. All transformed herbicide-resistant plants were phenotypically normal and exhibited genomic stability, as were the untransformed plants analysed by flow cytometry. Under greenhouse conditions, they grew to maturity, flowered and set seeds. Stable integration and expression of the bar gene in T0 and T1 plants were confirmed by Southern and Western blot analysis, while integration of the reporter uidA gene did not occur. The bar gene was inherited in a Mendelian fashion by the progeny, as detected by PPT resistance. The production of PPT-resistant plants may have significant practical applications in weed control in fields of L. corniculatus.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin",
pages = "493-475",
number = "3",
volume = "163",
doi = "10.1111/aab.12071"
}

Nikolić, R., Zdravkovic-Korac, S., Ninković, S., Dragicević, M., Miljuš-Đukić, J., Banović Đeri, B., Bohanec, B., Savić, J.,& Mitić, N.. (2013). Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin. in Annals of Applied Biology
Wiley, Hoboken., 163(3), 475-493.
https://doi.org/10.1111/aab.12071

Nikolić R, Zdravkovic-Korac S, Ninković S, Dragicević M, Miljuš-Đukić J, Banović Đeri B, Bohanec B, Savić J, Mitić N. Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin. in Annals of Applied Biology. 2013;163(3):475-493.
doi:10.1111/aab.12071 .

Nikolić, R., Zdravkovic-Korac, S., Ninković, S., Dragicević, M., Miljuš-Đukić, Jovanka, Banović Đeri, Bojana, Bohanec, B., Savić, Jelena, Mitić, N., "Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin" in Annals of Applied Biology, 163, no. 3 (2013):475-493,
https://doi.org/10.1111/aab.12071 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Savić, Jelena
AU  - Jovanović, Živko
AU  - Miljuš-Đukić, Jovanka
AU  - Radović, Svetlana
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/580
AB  - Shoot cultures of rare Balkan hyperaccumulating species Alyssum markgrafii were subjected to high nickel concentrations of 1, 3, 5, and 8 mM. The effects of graded nickel concentrations on toxicity, pigments, and several components of plant antioxidative defense system were characterized. Toxic effects of excess nickel were observed through slower growth and biomass decrease, together with increased reactive oxygen species (ROS) production and lipid peroxidation. Nickel exposure decreased chlorophyll a, b, total chlorophyll as well as carotenoid concentration. Addition of sodium benzoate, potent ROS scavenger, showed concentration-dependent disturbing effect on nickel hyperaccumulation, lowering the content of accumulated nickel in A. markgrafii shoots. General reducing power represented by low molecular weight antioxidants and phenols was inversely correlated with nickel concentration. Among the investigated antioxidative enzymes, POD seems to play important role in ROS level regulation upon excessive nickel in medium.
PB  - Springer Heidelberg, Heidelberg
T2  - Acta Physiologiae Plantarum
T1  - Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level
EP  - 2006
IS  - 5
SP  - 1997
VL  - 34
DO  - 10.1007/s11738-012-1001-3
ER  - 

@article{
author = "Stanisavljević, Nemanja and Savić, Jelena and Jovanović, Živko and Miljuš-Đukić, Jovanka and Radović, Svetlana and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2012",
abstract = "Shoot cultures of rare Balkan hyperaccumulating species Alyssum markgrafii were subjected to high nickel concentrations of 1, 3, 5, and 8 mM. The effects of graded nickel concentrations on toxicity, pigments, and several components of plant antioxidative defense system were characterized. Toxic effects of excess nickel were observed through slower growth and biomass decrease, together with increased reactive oxygen species (ROS) production and lipid peroxidation. Nickel exposure decreased chlorophyll a, b, total chlorophyll as well as carotenoid concentration. Addition of sodium benzoate, potent ROS scavenger, showed concentration-dependent disturbing effect on nickel hyperaccumulation, lowering the content of accumulated nickel in A. markgrafii shoots. General reducing power represented by low molecular weight antioxidants and phenols was inversely correlated with nickel concentration. Among the investigated antioxidative enzymes, POD seems to play important role in ROS level regulation upon excessive nickel in medium.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Acta Physiologiae Plantarum",
title = "Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level",
pages = "2006-1997",
number = "5",
volume = "34",
doi = "10.1007/s11738-012-1001-3"
}

Stanisavljević, N., Savić, J., Jovanović, Ž., Miljuš-Đukić, J., Radović, S., Vinterhalter, D.,& Vinterhalter, B.. (2012). Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level. in Acta Physiologiae Plantarum
Springer Heidelberg, Heidelberg., 34(5), 1997-2006.
https://doi.org/10.1007/s11738-012-1001-3

Stanisavljević N, Savić J, Jovanović Ž, Miljuš-Đukić J, Radović S, Vinterhalter D, Vinterhalter B. Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level. in Acta Physiologiae Plantarum. 2012;34(5):1997-2006.
doi:10.1007/s11738-012-1001-3 .

Stanisavljević, Nemanja, Savić, Jelena, Jovanović, Živko, Miljuš-Đukić, Jovanka, Radović, Svetlana, Vinterhalter, Dragan, Vinterhalter, Branka, "Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level" in Acta Physiologiae Plantarum, 34, no. 5 (2012):1997-2006,
https://doi.org/10.1007/s11738-012-1001-3 . .