TY  - JOUR
AU  - Ušjak, Dušan
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Kojić, Milan
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina T.
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1600
AB  - Aims To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results Chequerboard and time-kill assays were employed to explore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16-256 mu g ml(-1)). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004-0.035) of colistin and SeNPs against colistin-resistant isolates was revealed. Colistin (0.5 or 1 mu g ml(-1)) used in combination with SeNPs (0.5 mu g ml(-1)) was able to reduce initial inoculum during the first 4 h of incubation, in contrast to colistin (0.5, 1 or 2 mu g ml(-1)) alone. Conclusions These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles
EP  - 1206
IS  - 3
SP  - 1197
VL  - 133
DO  - 10.1111/jam.15638
ER  - 

@article{
author = "Ušjak, Dušan and Novović, Katarina and Filipić, Brankica and Kojić, Milan and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina T.",
year = "2022",
abstract = "Aims To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results Chequerboard and time-kill assays were employed to explore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16-256 mu g ml(-1)). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004-0.035) of colistin and SeNPs against colistin-resistant isolates was revealed. Colistin (0.5 or 1 mu g ml(-1)) used in combination with SeNPs (0.5 mu g ml(-1)) was able to reduce initial inoculum during the first 4 h of incubation, in contrast to colistin (0.5, 1 or 2 mu g ml(-1)) alone. Conclusions These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles",
pages = "1206-1197",
number = "3",
volume = "133",
doi = "10.1111/jam.15638"
}

Ušjak, D., Novović, K., Filipić, B., Kojić, M., Filipović, N., Stevanović, M.,& Milenković, M. T.. (2022). In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology
Wiley, Hoboken., 133(3), 1197-1206.
https://doi.org/10.1111/jam.15638

Ušjak D, Novović K, Filipić B, Kojić M, Filipović N, Stevanović M, Milenković MT. In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology. 2022;133(3):1197-1206.
doi:10.1111/jam.15638 .

Ušjak, Dušan, Novović, Katarina, Filipić, Brankica, Kojić, Milan, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina T., "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles" in Journal of Applied Microbiology, 133, no. 3 (2022):1197-1206,
https://doi.org/10.1111/jam.15638 . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Tolinački, Maja
AU  - Golić, Nataša
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1464
AB  - The present study is focused on the safety, technological characteristics, and probiotic evaluation of Enterococcus species from different artisanal raw milk dairy products, mainly cheeses with ripening. Apart from proteolytic and lipolytic activities, most enterococci show the ability to metabolize citrate and convert it to various aromatic compounds. Long-ripened cheeses therefore have a specific flavor that makes them different from cheeses produced from thermally treated milk with commercial starter cultures. In addition, enterococci are producers of bacteriocins effective against spoilage and pathogenic bacteria, so they can be used as food preservatives. However, the use of enterococci in the dairy industry should be approached with caution. Although originating from food, enterococci strains may carry various virulence factors and antibiotic-resistance genes and can have many adverse effects on human health. Still, despite their controversial status, the use of enterococci in the food industry is not strictly regulated since the existence of these so-called desirable and undesirable traits in enterococci is a strain-dependent characteristic. To be specific, the results of many studies showed that there are some enterococci strains that are safe for use as starter cultures or as probiotics since they do not carry virulence factors and antibiotic-resistance genes. These strains even exhibit strong health-promoting effects such as stimulation of the immune response, anti-inflammatory activity, hypocholesterolemic action, and usefulness in prevention/treatment of some diseases.
PB  - MDPI, Basel
T2  - Foods
T1  - Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns
IS  - 11
VL  - 10
DO  - 10.3390/foods10112753
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Popović, Nikola and Tolinački, Maja and Golić, Nataša",
year = "2021",
abstract = "The present study is focused on the safety, technological characteristics, and probiotic evaluation of Enterococcus species from different artisanal raw milk dairy products, mainly cheeses with ripening. Apart from proteolytic and lipolytic activities, most enterococci show the ability to metabolize citrate and convert it to various aromatic compounds. Long-ripened cheeses therefore have a specific flavor that makes them different from cheeses produced from thermally treated milk with commercial starter cultures. In addition, enterococci are producers of bacteriocins effective against spoilage and pathogenic bacteria, so they can be used as food preservatives. However, the use of enterococci in the dairy industry should be approached with caution. Although originating from food, enterococci strains may carry various virulence factors and antibiotic-resistance genes and can have many adverse effects on human health. Still, despite their controversial status, the use of enterococci in the food industry is not strictly regulated since the existence of these so-called desirable and undesirable traits in enterococci is a strain-dependent characteristic. To be specific, the results of many studies showed that there are some enterococci strains that are safe for use as starter cultures or as probiotics since they do not carry virulence factors and antibiotic-resistance genes. These strains even exhibit strong health-promoting effects such as stimulation of the immune response, anti-inflammatory activity, hypocholesterolemic action, and usefulness in prevention/treatment of some diseases.",
publisher = "MDPI, Basel",
journal = "Foods",
title = "Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns",
number = "11",
volume = "10",
doi = "10.3390/foods10112753"
}

Terzić-Vidojević, A., Veljović, K., Popović, N., Tolinački, M.,& Golić, N.. (2021). Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns. in Foods
MDPI, Basel., 10(11).
https://doi.org/10.3390/foods10112753

Terzić-Vidojević A, Veljović K, Popović N, Tolinački M, Golić N. Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns. in Foods. 2021;10(11).
doi:10.3390/foods10112753 .

Terzić-Vidojević, Amarela, Veljović, Katarina, Popović, Nikola, Tolinački, Maja, Golić, Nataša, "Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns" in Foods, 10, no. 11 (2021),
https://doi.org/10.3390/foods10112753 . .

TY  - JOUR
AU  - Mirković, Nemanja
AU  - Obradović, Mina
AU  - O'Connor, Paula M.
AU  - Filipić, Brankica
AU  - Jovčić, Branko
AU  - Cotter, Paul D.
AU  - Kojić, Milan
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1429
AB  - Screening for producers of potent antimicrobial peptides, resulted in the isolation of Bacillus cereus BGNM1 with strong antimicrobial activity against Listeria monocytogenes. Genome sequence analysis revealed that BGNM1 contains the gene cluster associated with the production of the lantibiotic, thusin, previously identified in B. thuringiensis. Purification of the antimicrobial activity confirmed that strain BGMN1 produces thusin. Both thusin sensitive and resistant strains were detected among clinical isolates of Streptococcus agalactiae. Random mutagenesis of a thusin sensitive strain, S. agalactiae B782, was performed in an attempt to identify the receptor protein for thusin. Three independent thusin resistant mutants were selected and their complete genomes sequenced. Comparative sequence analysis of these mutants with the WT strain revealed that duplication of a region encoding a 79 amino acids repeat in a C-protein alpha-antigen was a common difference, suggesting it to be responsible for increased resistance to thusin. Since induced thusin resistant mutants showed higher level of resistance than the naturally resistant B761 strain, complete genome sequencing of strain B761 was performed to check the integrity of the C-protein alpha-antigen-encoding gene. This analysis revealed that this gene is deleted in B761, providing further evidence that this protein promotes interaction of the thusin with receptor.
PB  - Springer, Dordrecht
T2  - Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
T1  - C-protein alpha-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae
EP  - 1607
IS  - 10
SP  - 1595
VL  - 114
DO  - 10.1007/s10482-021-01626-3
ER  - 

@article{
author = "Mirković, Nemanja and Obradović, Mina and O'Connor, Paula M. and Filipić, Brankica and Jovčić, Branko and Cotter, Paul D. and Kojić, Milan",
year = "2021",
abstract = "Screening for producers of potent antimicrobial peptides, resulted in the isolation of Bacillus cereus BGNM1 with strong antimicrobial activity against Listeria monocytogenes. Genome sequence analysis revealed that BGNM1 contains the gene cluster associated with the production of the lantibiotic, thusin, previously identified in B. thuringiensis. Purification of the antimicrobial activity confirmed that strain BGMN1 produces thusin. Both thusin sensitive and resistant strains were detected among clinical isolates of Streptococcus agalactiae. Random mutagenesis of a thusin sensitive strain, S. agalactiae B782, was performed in an attempt to identify the receptor protein for thusin. Three independent thusin resistant mutants were selected and their complete genomes sequenced. Comparative sequence analysis of these mutants with the WT strain revealed that duplication of a region encoding a 79 amino acids repeat in a C-protein alpha-antigen was a common difference, suggesting it to be responsible for increased resistance to thusin. Since induced thusin resistant mutants showed higher level of resistance than the naturally resistant B761 strain, complete genome sequencing of strain B761 was performed to check the integrity of the C-protein alpha-antigen-encoding gene. This analysis revealed that this gene is deleted in B761, providing further evidence that this protein promotes interaction of the thusin with receptor.",
publisher = "Springer, Dordrecht",
journal = "Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology",
title = "C-protein alpha-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae",
pages = "1607-1595",
number = "10",
volume = "114",
doi = "10.1007/s10482-021-01626-3"
}

Mirković, N., Obradović, M., O'Connor, P. M., Filipić, B., Jovčić, B., Cotter, P. D.,& Kojić, M.. (2021). C-protein alpha-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
Springer, Dordrecht., 114(10), 1595-1607.
https://doi.org/10.1007/s10482-021-01626-3

Mirković N, Obradović M, O'Connor PM, Filipić B, Jovčić B, Cotter PD, Kojić M. C-protein alpha-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. 2021;114(10):1595-1607.
doi:10.1007/s10482-021-01626-3 .

Mirković, Nemanja, Obradović, Mina, O'Connor, Paula M., Filipić, Brankica, Jovčić, Branko, Cotter, Paul D., Kojić, Milan, "C-protein alpha-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae" in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 114, no. 10 (2021):1595-1607,
https://doi.org/10.1007/s10482-021-01626-3 . .

TY  - JOUR
AU  - Ljubobratović, Uros
AU  - Fazekas, Georgina
AU  - Koljukaj, Alan
AU  - Ristović, Tijana
AU  - Vass, Vivien
AU  - Ardo, Laszlo
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Pesić, Mirjana
AU  - Milincić, Danijel
AU  - Kostić, Aleksandar
AU  - Lukić, Jovanka
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1448
AB  - This study evaluated whether inert feed enriched with Lb. paracasei subsp. paracasei BGHN14 may be used as a weaning diet for first feeding pike-perch larvae. Three experimental groups were weaned from the start of exogenous feeding: two groups were given inert feed enriched with BGHN14 either via 12 h incubation with live BGHN14 cells or via coating with homogenized BGHN14 cells and one group was supplemented non-enriched inert feed. In all three groups Artemia was co-fed with inert feed during weaning. Control group larvae were fed Artemia exclusively during the treatment period. Treatment lasted fourteen days, starting from the 6th day post-hatch (DPH). Larval sampling was performed on the 20th DPH for gene expression and enzyme activity analysis. Larvae were also sampled on the 32nd DPH for morphometric and body composition analysis. Our results showed that weaning of first feeding pike-perch larvae was associated with an increase of fish condition (0.72 +/- 0.12-0.77 +/- 0.11 versus 0.67 +/- 0.11 in controls), but it suppressed skeleton development, according to Col1 mRNA expression (1 +/- 0.51-1.06 +/- 0.36 versus 2.07 +/- 0.53 in controls) and reduced fat deposition (1.25 +/- 0.23-1.49 +/- 0.33 versus 1.84 +/- 0.31% in controls). This presumably reflected lower availability of soluble proteins in microdiet as opposed to live food, along with high leaching rate of amino acids from solid feed particles, as reported in our previous studies. However, skeleton differentiation was not impaired in group weaned on BGHN14 homogenate coated feed (Col1 mRNA expression: 2.68 +/- 0.72), which was enriched in skeleton building and taste stimulating amino acids. These larvae were also presented with substantially higher length (15.28 +/- 2.55 versus 13.93 +/- 2.31 mm in controls) and weight (26.56 +/- 13.83 versus 21.03 +/- 11.25 mg in controls), which correlated with lower trypsin activity (1.06 +/- 0.13 versus 1.43 +/- 0.26 mU/mg of proteins in controls) and an increase of PLA2 to trypsin activity ratio (453.12 +/- 109.36 versus 264.84 +/- 69.03 in controls). Present study suggests that weaning of first feeding pike-perch larvae using BGHN14 homogenate coated microdiet supports skeleton development and improves fish growth.
PB  - Elsevier, Amsterdam
T2  - Aquaculture
T1  - Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding
VL  - 542
DO  - 10.1016/j.aquaculture.2021.736901
ER  - 

@article{
author = "Ljubobratović, Uros and Fazekas, Georgina and Koljukaj, Alan and Ristović, Tijana and Vass, Vivien and Ardo, Laszlo and Stanisavljević, Nemanja and Vukotić, Goran and Pesić, Mirjana and Milincić, Danijel and Kostić, Aleksandar and Lukić, Jovanka",
year = "2021",
abstract = "This study evaluated whether inert feed enriched with Lb. paracasei subsp. paracasei BGHN14 may be used as a weaning diet for first feeding pike-perch larvae. Three experimental groups were weaned from the start of exogenous feeding: two groups were given inert feed enriched with BGHN14 either via 12 h incubation with live BGHN14 cells or via coating with homogenized BGHN14 cells and one group was supplemented non-enriched inert feed. In all three groups Artemia was co-fed with inert feed during weaning. Control group larvae were fed Artemia exclusively during the treatment period. Treatment lasted fourteen days, starting from the 6th day post-hatch (DPH). Larval sampling was performed on the 20th DPH for gene expression and enzyme activity analysis. Larvae were also sampled on the 32nd DPH for morphometric and body composition analysis. Our results showed that weaning of first feeding pike-perch larvae was associated with an increase of fish condition (0.72 +/- 0.12-0.77 +/- 0.11 versus 0.67 +/- 0.11 in controls), but it suppressed skeleton development, according to Col1 mRNA expression (1 +/- 0.51-1.06 +/- 0.36 versus 2.07 +/- 0.53 in controls) and reduced fat deposition (1.25 +/- 0.23-1.49 +/- 0.33 versus 1.84 +/- 0.31% in controls). This presumably reflected lower availability of soluble proteins in microdiet as opposed to live food, along with high leaching rate of amino acids from solid feed particles, as reported in our previous studies. However, skeleton differentiation was not impaired in group weaned on BGHN14 homogenate coated feed (Col1 mRNA expression: 2.68 +/- 0.72), which was enriched in skeleton building and taste stimulating amino acids. These larvae were also presented with substantially higher length (15.28 +/- 2.55 versus 13.93 +/- 2.31 mm in controls) and weight (26.56 +/- 13.83 versus 21.03 +/- 11.25 mg in controls), which correlated with lower trypsin activity (1.06 +/- 0.13 versus 1.43 +/- 0.26 mU/mg of proteins in controls) and an increase of PLA2 to trypsin activity ratio (453.12 +/- 109.36 versus 264.84 +/- 69.03 in controls). Present study suggests that weaning of first feeding pike-perch larvae using BGHN14 homogenate coated microdiet supports skeleton development and improves fish growth.",
publisher = "Elsevier, Amsterdam",
journal = "Aquaculture",
title = "Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding",
volume = "542",
doi = "10.1016/j.aquaculture.2021.736901"
}

Ljubobratović, U., Fazekas, G., Koljukaj, A., Ristović, T., Vass, V., Ardo, L., Stanisavljević, N., Vukotić, G., Pesić, M., Milincić, D., Kostić, A.,& Lukić, J.. (2021). Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding. in Aquaculture
Elsevier, Amsterdam., 542.
https://doi.org/10.1016/j.aquaculture.2021.736901

Ljubobratović U, Fazekas G, Koljukaj A, Ristović T, Vass V, Ardo L, Stanisavljević N, Vukotić G, Pesić M, Milincić D, Kostić A, Lukić J. Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding. in Aquaculture. 2021;542.
doi:10.1016/j.aquaculture.2021.736901 .

Ljubobratović, Uros, Fazekas, Georgina, Koljukaj, Alan, Ristović, Tijana, Vass, Vivien, Ardo, Laszlo, Stanisavljević, Nemanja, Vukotić, Goran, Pesić, Mirjana, Milincić, Danijel, Kostić, Aleksandar, Lukić, Jovanka, "Pike-perch larvae growth in response to administration of lactobacilli-enriched inert feed during first feeding" in Aquaculture, 542 (2021),
https://doi.org/10.1016/j.aquaculture.2021.736901 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Terzić-Vidojević, Amarela
AU  - Begović, Jelena
AU  - Golić, Nataša
AU  - Ljubobratović, Uros
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1504
AB  - This research aimed to test whether coating with Lactobacillus paracasei subsp. paracasei BGHN14 homogenate may increase soluble protein and amino acid amount on larval fish feed surface. Total amino acid and protein, as well as taste stimulating and growth promoting amino acid amounts were analyzed in coated feed samples. Results indicated that coating with BGHN14 homogenate increased the amount of soluble proteins and free Glycine on feed surface, as well as the availability of protein-bound (hydroxy-)Proline. This lab-scale research provides the basis for use of lactobacilli as resource efficient source of soluble nutrients for animal feedstuffs.
PB  - Central Fisheries Research Inst, Trabzon
T2  - Turkish Journal of Fisheries and Aquatic Sciences
T1  - Enrichment of Larval Fish Feed with Free Amino Acids and Proteins by Coating with Lactobacillus paracasei subsp. paracasei BGHN14 Homogenate
EP  - 573
IS  - 11
SP  - 569
VL  - 21
DO  - 10.4194/1303-2712-v21_11_05
ER  - 

@article{
author = "Lukić, Jovanka and Stanisavljević, Nemanja and Vukotić, Goran and Terzić-Vidojević, Amarela and Begović, Jelena and Golić, Nataša and Ljubobratović, Uros",
year = "2021",
abstract = "This research aimed to test whether coating with Lactobacillus paracasei subsp. paracasei BGHN14 homogenate may increase soluble protein and amino acid amount on larval fish feed surface. Total amino acid and protein, as well as taste stimulating and growth promoting amino acid amounts were analyzed in coated feed samples. Results indicated that coating with BGHN14 homogenate increased the amount of soluble proteins and free Glycine on feed surface, as well as the availability of protein-bound (hydroxy-)Proline. This lab-scale research provides the basis for use of lactobacilli as resource efficient source of soluble nutrients for animal feedstuffs.",
publisher = "Central Fisheries Research Inst, Trabzon",
journal = "Turkish Journal of Fisheries and Aquatic Sciences",
title = "Enrichment of Larval Fish Feed with Free Amino Acids and Proteins by Coating with Lactobacillus paracasei subsp. paracasei BGHN14 Homogenate",
pages = "573-569",
number = "11",
volume = "21",
doi = "10.4194/1303-2712-v21_11_05"
}

Lukić, J., Stanisavljević, N., Vukotić, G., Terzić-Vidojević, A., Begović, J., Golić, N.,& Ljubobratović, U.. (2021). Enrichment of Larval Fish Feed with Free Amino Acids and Proteins by Coating with Lactobacillus paracasei subsp. paracasei BGHN14 Homogenate. in Turkish Journal of Fisheries and Aquatic Sciences
Central Fisheries Research Inst, Trabzon., 21(11), 569-573.
https://doi.org/10.4194/1303-2712-v21_11_05

Lukić J, Stanisavljević N, Vukotić G, Terzić-Vidojević A, Begović J, Golić N, Ljubobratović U. Enrichment of Larval Fish Feed with Free Amino Acids and Proteins by Coating with Lactobacillus paracasei subsp. paracasei BGHN14 Homogenate. in Turkish Journal of Fisheries and Aquatic Sciences. 2021;21(11):569-573.
doi:10.4194/1303-2712-v21_11_05 .

Lukić, Jovanka, Stanisavljević, Nemanja, Vukotić, Goran, Terzić-Vidojević, Amarela, Begović, Jelena, Golić, Nataša, Ljubobratović, Uros, "Enrichment of Larval Fish Feed with Free Amino Acids and Proteins by Coating with Lactobacillus paracasei subsp. paracasei BGHN14 Homogenate" in Turkish Journal of Fisheries and Aquatic Sciences, 21, no. 11 (2021):569-573,
https://doi.org/10.4194/1303-2712-v21_11_05 . .

TY  - JOUR
AU  - Soković Bajić, Svetlana
AU  - Mihajlović, Sanja B.
AU  - Radojević, Dušan
AU  - Popović, Dušanka
AU  - Đokić, Jelena
AU  - Stanisavljević, Suzana
AU  - Lazarević, Milica N.
AU  - Miljković, Djordje M.
AU  - Ruas-Madiedo, Patricia
AU  - Golić, Nataša
AU  - Tolinački, Maja
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1386
AB  - Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system. The aim of this work was to study the probiotic effect of gamma-aminobutyric acid (GABA)-producer Lactobacillus brevis BGZLS10-17 on experimental autoimmune encephalomyelitis (EAE), an experimental animal MS model. Clinical EAE symptoms were monitored in Dark Agouti (DA) rats treated with L. brevis BGZLS10-17 strain, or supernatant obtained from 48 h culture of L. brevis BGZLS10-17 cultivated in growth medium with or without GABA precursor monosodium glutamate (MSG). The results revealed that oral administration of L. brevis BGZLS10-17 alleviates the symptoms of EAE in DA rats. Namely, treatment with BGZLS10-17 and the supernatant of the strain cultivated in medium with MSG delayed the onset, shortened the duration, and reduced the intensity of the disease in the period when the EAE symptoms in controls were most pronounced. The probiotic treated animals were completely recovered after forty days, unlike the control animals. The results indicate that supplementation with live strain or with supernatant containing GABA produced by L. brevis BGZLS10-17 could alleviate the EAE symptoms. However, the use of L. brevis BGZLS10-17 in functional food as probiotic for autoimmune diseases should be tested in clinical trials.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Characterization of pH resistance and the proteolytic activity of GABA producing Lactobacillus brevis BGZLS10-17 in preparation of fermented milk beverage and the effects on the symptoms of the experimental autoimmune encephalomyelitis
EP  - 176
IS  - 2
SP  - 163
VL  - 85
DO  - 10.2298/JSC190716094S
ER  - 

@article{
author = "Soković Bajić, Svetlana and Mihajlović, Sanja B. and Radojević, Dušan and Popović, Dušanka and Đokić, Jelena and Stanisavljević, Suzana and Lazarević, Milica N. and Miljković, Djordje M. and Ruas-Madiedo, Patricia and Golić, Nataša and Tolinački, Maja",
year = "2020",
abstract = "Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system. The aim of this work was to study the probiotic effect of gamma-aminobutyric acid (GABA)-producer Lactobacillus brevis BGZLS10-17 on experimental autoimmune encephalomyelitis (EAE), an experimental animal MS model. Clinical EAE symptoms were monitored in Dark Agouti (DA) rats treated with L. brevis BGZLS10-17 strain, or supernatant obtained from 48 h culture of L. brevis BGZLS10-17 cultivated in growth medium with or without GABA precursor monosodium glutamate (MSG). The results revealed that oral administration of L. brevis BGZLS10-17 alleviates the symptoms of EAE in DA rats. Namely, treatment with BGZLS10-17 and the supernatant of the strain cultivated in medium with MSG delayed the onset, shortened the duration, and reduced the intensity of the disease in the period when the EAE symptoms in controls were most pronounced. The probiotic treated animals were completely recovered after forty days, unlike the control animals. The results indicate that supplementation with live strain or with supernatant containing GABA produced by L. brevis BGZLS10-17 could alleviate the EAE symptoms. However, the use of L. brevis BGZLS10-17 in functional food as probiotic for autoimmune diseases should be tested in clinical trials.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Characterization of pH resistance and the proteolytic activity of GABA producing Lactobacillus brevis BGZLS10-17 in preparation of fermented milk beverage and the effects on the symptoms of the experimental autoimmune encephalomyelitis",
pages = "176-163",
number = "2",
volume = "85",
doi = "10.2298/JSC190716094S"
}

Soković Bajić, S., Mihajlović, S. B., Radojević, D., Popović, D., Đokić, J., Stanisavljević, S., Lazarević, M. N., Miljković, D. M., Ruas-Madiedo, P., Golić, N.,& Tolinački, M.. (2020). Characterization of pH resistance and the proteolytic activity of GABA producing Lactobacillus brevis BGZLS10-17 in preparation of fermented milk beverage and the effects on the symptoms of the experimental autoimmune encephalomyelitis. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 85(2), 163-176.
https://doi.org/10.2298/JSC190716094S

Soković Bajić S, Mihajlović SB, Radojević D, Popović D, Đokić J, Stanisavljević S, Lazarević MN, Miljković DM, Ruas-Madiedo P, Golić N, Tolinački M. Characterization of pH resistance and the proteolytic activity of GABA producing Lactobacillus brevis BGZLS10-17 in preparation of fermented milk beverage and the effects on the symptoms of the experimental autoimmune encephalomyelitis. in Journal of the Serbian Chemical Society. 2020;85(2):163-176.
doi:10.2298/JSC190716094S .

Soković Bajić, Svetlana, Mihajlović, Sanja B., Radojević, Dušan, Popović, Dušanka, Đokić, Jelena, Stanisavljević, Suzana, Lazarević, Milica N., Miljković, Djordje M., Ruas-Madiedo, Patricia, Golić, Nataša, Tolinački, Maja, "Characterization of pH resistance and the proteolytic activity of GABA producing Lactobacillus brevis BGZLS10-17 in preparation of fermented milk beverage and the effects on the symptoms of the experimental autoimmune encephalomyelitis" in Journal of the Serbian Chemical Society, 85, no. 2 (2020):163-176,
https://doi.org/10.2298/JSC190716094S . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Novović, Katarina
AU  - Studholme, David J.
AU  - Malešević, Milka
AU  - Mirković, Nemanja
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1306
AB  - Long-term overuse of antibiotics has driven the propagation and spreading of antibiotic resistance genes (ARGs) such as efflux pumps in the environment, which can be transferred to clinically relevant pathogens. This study explored the abundance and diversity of ARGs and mobile genetic elements within bacterial communities from sediments of three Western Balkans glacial lakes: Plav Lake (high impact of human population), Black Lake (medium impact of human population) and Donje Bare Lake (remote lake, minimal impact of human population) via shotgun metagenomics. Assembled metagenomic sequences revealed that Resistance-Nodulation-Division (RND) efflux pumps genes were most abundant in metagenome from the Plav Lake. The Integron Finder bioinformatics tool detected 38clusters ofattCsiteslackingintegron-integrases (CALIN) elements: 20 from Plav Lake, four from Black Lake and 14 from Donje Bare Lake. A complete integron sequence was recovered only from the assembled metagenome from Plav Lake. Plasmid contents within the metagenomes were similar, with proportions of contigs being plasmid-related: 1.73% for Plav Lake, 1.59% for Black Lake and 1.64% for Donje Bare Lake. The investigation showed that RNDs and mobile genetic elements content correlated with human population impact.
PB  - IWA Publishing, London
T2  - Journal of Water and Health
T1  - Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments
EP  - 397
IS  - 3
SP  - 383
VL  - 18
DO  - 10.2166/wh.2020.227
ER  - 

@article{
author = "Filipić, Brankica and Novović, Katarina and Studholme, David J. and Malešević, Milka and Mirković, Nemanja and Kojić, Milan and Jovčić, Branko",
year = "2020",
abstract = "Long-term overuse of antibiotics has driven the propagation and spreading of antibiotic resistance genes (ARGs) such as efflux pumps in the environment, which can be transferred to clinically relevant pathogens. This study explored the abundance and diversity of ARGs and mobile genetic elements within bacterial communities from sediments of three Western Balkans glacial lakes: Plav Lake (high impact of human population), Black Lake (medium impact of human population) and Donje Bare Lake (remote lake, minimal impact of human population) via shotgun metagenomics. Assembled metagenomic sequences revealed that Resistance-Nodulation-Division (RND) efflux pumps genes were most abundant in metagenome from the Plav Lake. The Integron Finder bioinformatics tool detected 38clusters ofattCsiteslackingintegron-integrases (CALIN) elements: 20 from Plav Lake, four from Black Lake and 14 from Donje Bare Lake. A complete integron sequence was recovered only from the assembled metagenome from Plav Lake. Plasmid contents within the metagenomes were similar, with proportions of contigs being plasmid-related: 1.73% for Plav Lake, 1.59% for Black Lake and 1.64% for Donje Bare Lake. The investigation showed that RNDs and mobile genetic elements content correlated with human population impact.",
publisher = "IWA Publishing, London",
journal = "Journal of Water and Health",
title = "Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments",
pages = "397-383",
number = "3",
volume = "18",
doi = "10.2166/wh.2020.227"
}

Filipić, B., Novović, K., Studholme, D. J., Malešević, M., Mirković, N., Kojić, M.,& Jovčić, B.. (2020). Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments. in Journal of Water and Health
IWA Publishing, London., 18(3), 383-397.
https://doi.org/10.2166/wh.2020.227

Filipić B, Novović K, Studholme DJ, Malešević M, Mirković N, Kojić M, Jovčić B. Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments. in Journal of Water and Health. 2020;18(3):383-397.
doi:10.2166/wh.2020.227 .

Filipić, Brankica, Novović, Katarina, Studholme, David J., Malešević, Milka, Mirković, Nemanja, Kojić, Milan, Jovčić, Branko, "Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments" in Journal of Water and Health, 18, no. 3 (2020):383-397,
https://doi.org/10.2166/wh.2020.227 . .

TY  - JOUR
AU  - Kojić, Milan
AU  - Jovčić, Branko
AU  - Miljković, Marija
AU  - Novović, Katarina
AU  - Begović, Jelena
AU  - Studholme, David J.
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1373
AB  - Bacteria can gain resistance to antimicrobials by acquiring and expressing genetic elements that encode resistance determinants such as efflux pumps and drug-modifying enzymes, thus hampering treatment of infection. Previously we showed that acquisition of spectinomycin resistance in a lactococcal strain was correlated with a reversible genomic inversion, but the precise location and the genes affected were unknown. Here we use long read whole-genome sequencing to precisely define the genomic inversion and we use quantitative PCR to identify associated changes in gene expression levels. The boundaries of the inversion fall within two identical copies of a prophage-like sequence, located on the left and right replichores; this suggests possible mechanisms for inversion through homologous recombination or prophage activity. The inversion is asymmetrical in respect of the axis between the origin and terminus of the replication and modulates the expression of a SAM-dependent methyltransferase, whose heterologous expression confers resistance to spectinomycin in lactococci and that is up regulated on exposure to spectinomycin. This study provides one of the first examples of phase variation via large-scale chromosomal inversions that confers a switch in antimicrobial resistance in bacteria and the first outside of Staphylococcus aureus.
PB  - Elsevier Gmbh, Munich
T2  - Microbiological Research
T1  - Large-scale chromosome flip-flop reversible inversion mediates phenotypic switching of expression of antibiotic resistance in lactococci
VL  - 241
DO  - 10.1016/j.micres.2020.126583
ER  - 

@article{
author = "Kojić, Milan and Jovčić, Branko and Miljković, Marija and Novović, Katarina and Begović, Jelena and Studholme, David J.",
year = "2020",
abstract = "Bacteria can gain resistance to antimicrobials by acquiring and expressing genetic elements that encode resistance determinants such as efflux pumps and drug-modifying enzymes, thus hampering treatment of infection. Previously we showed that acquisition of spectinomycin resistance in a lactococcal strain was correlated with a reversible genomic inversion, but the precise location and the genes affected were unknown. Here we use long read whole-genome sequencing to precisely define the genomic inversion and we use quantitative PCR to identify associated changes in gene expression levels. The boundaries of the inversion fall within two identical copies of a prophage-like sequence, located on the left and right replichores; this suggests possible mechanisms for inversion through homologous recombination or prophage activity. The inversion is asymmetrical in respect of the axis between the origin and terminus of the replication and modulates the expression of a SAM-dependent methyltransferase, whose heterologous expression confers resistance to spectinomycin in lactococci and that is up regulated on exposure to spectinomycin. This study provides one of the first examples of phase variation via large-scale chromosomal inversions that confers a switch in antimicrobial resistance in bacteria and the first outside of Staphylococcus aureus.",
publisher = "Elsevier Gmbh, Munich",
journal = "Microbiological Research",
title = "Large-scale chromosome flip-flop reversible inversion mediates phenotypic switching of expression of antibiotic resistance in lactococci",
volume = "241",
doi = "10.1016/j.micres.2020.126583"
}

Kojić, M., Jovčić, B., Miljković, M., Novović, K., Begović, J.,& Studholme, D. J.. (2020). Large-scale chromosome flip-flop reversible inversion mediates phenotypic switching of expression of antibiotic resistance in lactococci. in Microbiological Research
Elsevier Gmbh, Munich., 241.
https://doi.org/10.1016/j.micres.2020.126583

Kojić M, Jovčić B, Miljković M, Novović K, Begović J, Studholme DJ. Large-scale chromosome flip-flop reversible inversion mediates phenotypic switching of expression of antibiotic resistance in lactococci. in Microbiological Research. 2020;241.
doi:10.1016/j.micres.2020.126583 .

Kojić, Milan, Jovčić, Branko, Miljković, Marija, Novović, Katarina, Begović, Jelena, Studholme, David J., "Large-scale chromosome flip-flop reversible inversion mediates phenotypic switching of expression of antibiotic resistance in lactococci" in Microbiological Research, 241 (2020),
https://doi.org/10.1016/j.micres.2020.126583 . .

TY  - JOUR
AU  - Bojović, Katarina
AU  - Ignjatović, Durdica
AU  - Soković Bajić, Svetlana
AU  - Vojnovic Milutinović, Danijela
AU  - Tomić, Mirko
AU  - Golić, Nataša
AU  - Tolinački, Maja
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1316
AB  - While gut microbiota dysbiosis has been linked with autism, its role in the etiology of other neurodevelopmental disorders (NDD) is largely underexplored. To our knowledge this is the first study to evaluate gut microbiota diversity and composition in 36 children from the Republic of Serbia diagnosed with NDD and 28 healthy children. The results revealed an increased incidence of potentially harmful bacteria, closely related to Clostridium species, in the NDD patient group compared to the Control group: Desulfotomaculum guttoideum (P  lt  0.01), Intestinibacter bartlettii (P  lt  0.05), and Romboutsia ilealis (P  lt  0.001). On the other hand, significantly lower diversity of common commensal bacteria in the NDD group of patients was noticed. Enterococcus faecalis (P  lt  0.05), Enterococcus gallinarum (P  lt  0.01), Streptococcus pasteurianus (P  lt  0.05), Lactobacillus rhamnosus (P  lt  0.01) and Bifidobacteria sp. were detected in lower numbers of patients or were even absent in some NDD patients. In addition, butyrate-producing bacteria Faecalibacterium prausnitzii (P  lt  0.01), Butyricicoccus pullicaecorum (P  lt  0.05), and Eubacterium rectale (P = 0.07) were less frequent in the NDD patient group. In line with that, the levels of fecal short chain fatty acids (SCFAs) were determined. Although significant differences in SCFA levels were not detected between NDD patients and the Control group, a positive correlation was noted between number of rDNA amplicons obtained with universal primers and level of propionic acid, as well as a trend for levels of total SCFAs and butyric acid in the Control group. This correlation is lost in the NDD patient group, indicating that NDD patients' microbiota differs from the microbiota of healthy children in the presence or number of strong SCFA-producing bacteria. According to a range-weighted richness index it was observed that microbial diversity was significantly lower in the NDD patient group. Our study reveals that the intestinal microbiota from NDD patients differs from the microbiota of healthy children. It is hypothesized that early life microbiome might have an impact on GI disturbances and accompanied behavioral problems frequently observed in patients with a broad spectrum of NDD.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Cellular and Infection Microbiology
T1  - Gut Microbiota Dysbiosis Associated With Altered Production of Short Chain Fatty Acids in Children With Neurodevelopmental Disorders
VL  - 10
DO  - 10.3389/fcimb.2020.00223
ER  - 

@article{
author = "Bojović, Katarina and Ignjatović, Durdica and Soković Bajić, Svetlana and Vojnovic Milutinović, Danijela and Tomić, Mirko and Golić, Nataša and Tolinački, Maja",
year = "2020",
abstract = "While gut microbiota dysbiosis has been linked with autism, its role in the etiology of other neurodevelopmental disorders (NDD) is largely underexplored. To our knowledge this is the first study to evaluate gut microbiota diversity and composition in 36 children from the Republic of Serbia diagnosed with NDD and 28 healthy children. The results revealed an increased incidence of potentially harmful bacteria, closely related to Clostridium species, in the NDD patient group compared to the Control group: Desulfotomaculum guttoideum (P  lt  0.01), Intestinibacter bartlettii (P  lt  0.05), and Romboutsia ilealis (P  lt  0.001). On the other hand, significantly lower diversity of common commensal bacteria in the NDD group of patients was noticed. Enterococcus faecalis (P  lt  0.05), Enterococcus gallinarum (P  lt  0.01), Streptococcus pasteurianus (P  lt  0.05), Lactobacillus rhamnosus (P  lt  0.01) and Bifidobacteria sp. were detected in lower numbers of patients or were even absent in some NDD patients. In addition, butyrate-producing bacteria Faecalibacterium prausnitzii (P  lt  0.01), Butyricicoccus pullicaecorum (P  lt  0.05), and Eubacterium rectale (P = 0.07) were less frequent in the NDD patient group. In line with that, the levels of fecal short chain fatty acids (SCFAs) were determined. Although significant differences in SCFA levels were not detected between NDD patients and the Control group, a positive correlation was noted between number of rDNA amplicons obtained with universal primers and level of propionic acid, as well as a trend for levels of total SCFAs and butyric acid in the Control group. This correlation is lost in the NDD patient group, indicating that NDD patients' microbiota differs from the microbiota of healthy children in the presence or number of strong SCFA-producing bacteria. According to a range-weighted richness index it was observed that microbial diversity was significantly lower in the NDD patient group. Our study reveals that the intestinal microbiota from NDD patients differs from the microbiota of healthy children. It is hypothesized that early life microbiome might have an impact on GI disturbances and accompanied behavioral problems frequently observed in patients with a broad spectrum of NDD.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Cellular and Infection Microbiology",
title = "Gut Microbiota Dysbiosis Associated With Altered Production of Short Chain Fatty Acids in Children With Neurodevelopmental Disorders",
volume = "10",
doi = "10.3389/fcimb.2020.00223"
}

Bojović, K., Ignjatović, D., Soković Bajić, S., Vojnovic Milutinović, D., Tomić, M., Golić, N.,& Tolinački, M.. (2020). Gut Microbiota Dysbiosis Associated With Altered Production of Short Chain Fatty Acids in Children With Neurodevelopmental Disorders. in Frontiers in Cellular and Infection Microbiology
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fcimb.2020.00223

Bojović K, Ignjatović D, Soković Bajić S, Vojnovic Milutinović D, Tomić M, Golić N, Tolinački M. Gut Microbiota Dysbiosis Associated With Altered Production of Short Chain Fatty Acids in Children With Neurodevelopmental Disorders. in Frontiers in Cellular and Infection Microbiology. 2020;10.
doi:10.3389/fcimb.2020.00223 .

Bojović, Katarina, Ignjatović, Durdica, Soković Bajić, Svetlana, Vojnovic Milutinović, Danijela, Tomić, Mirko, Golić, Nataša, Tolinački, Maja, "Gut Microbiota Dysbiosis Associated With Altered Production of Short Chain Fatty Acids in Children With Neurodevelopmental Disorders" in Frontiers in Cellular and Infection Microbiology, 10 (2020),
https://doi.org/10.3389/fcimb.2020.00223 . .

TY  - JOUR
AU  - Soković Bajić, Svetlana
AU  - Đokić, Jelena
AU  - Dinić, Miroslav
AU  - Tomić, Sergej
AU  - Popović, Nikola
AU  - Brdarić, Emilija
AU  - Golić, Nataša
AU  - Tolinački, Maja
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1318
AB  - The characterization of mechanisms involved in the positive effects of probiotic bacteria in various pathophysiological conditions is a prerogative for their safe and efficient application in biomedicine. We have investigated the immunological effects of live bacteria-free supernatant collected from GABA-producing Lactobacillus brevis BGZLS10-17 on Concanavalin A-stimulated mesenteric lymph node cells (MLNC), an in vitro model of activated immune cells. We have shown that GABA containing and GABA-free supernatant of Lactobacillus brevis BGZLS10-17 have strong immunoregulatory effects on MLNC. Further, GABA produced by this strain exhibit additional inhibitory effects on proliferation, IFN-gamma and IL-17 production by MLNC, and the expression of MHCII and CD80 on antigen presenting cells. At the other hand, GABA-containing supernatants displayed the strongest stimulatory effects on the expression of immunoregulatory molecules, such as Foxp3(+), IL-10, TGF-beta, CTLA4 and SIRP-alpha. By looking for the mechanisms of actions, we found that supernatants produced by BGZLS10-17 induce autophagy in different MLNC, such as CD4(+) and CD8(+) T lymphocytes, NK and NKT cells, as well as antigen presenting cells. Further, we showed that the stimulation of Foxp3(+), IL-10 and TGF-beta expression by BGZLS10-17 produced GABA is completely mediated by the induction of ATG5 dependent autophagy, and that other molecules in the supernatants display GABA-, ATG5-, Foxp3(+)-, IL-10- and TGF-beta- independent, immunoregulatory effects.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro
SP  - 1347
VL  - 10
DO  - 10.1038/s41598-020-58177-2
ER  - 

@article{
author = "Soković Bajić, Svetlana and Đokić, Jelena and Dinić, Miroslav and Tomić, Sergej and Popović, Nikola and Brdarić, Emilija and Golić, Nataša and Tolinački, Maja",
year = "2020",
abstract = "The characterization of mechanisms involved in the positive effects of probiotic bacteria in various pathophysiological conditions is a prerogative for their safe and efficient application in biomedicine. We have investigated the immunological effects of live bacteria-free supernatant collected from GABA-producing Lactobacillus brevis BGZLS10-17 on Concanavalin A-stimulated mesenteric lymph node cells (MLNC), an in vitro model of activated immune cells. We have shown that GABA containing and GABA-free supernatant of Lactobacillus brevis BGZLS10-17 have strong immunoregulatory effects on MLNC. Further, GABA produced by this strain exhibit additional inhibitory effects on proliferation, IFN-gamma and IL-17 production by MLNC, and the expression of MHCII and CD80 on antigen presenting cells. At the other hand, GABA-containing supernatants displayed the strongest stimulatory effects on the expression of immunoregulatory molecules, such as Foxp3(+), IL-10, TGF-beta, CTLA4 and SIRP-alpha. By looking for the mechanisms of actions, we found that supernatants produced by BGZLS10-17 induce autophagy in different MLNC, such as CD4(+) and CD8(+) T lymphocytes, NK and NKT cells, as well as antigen presenting cells. Further, we showed that the stimulation of Foxp3(+), IL-10 and TGF-beta expression by BGZLS10-17 produced GABA is completely mediated by the induction of ATG5 dependent autophagy, and that other molecules in the supernatants display GABA-, ATG5-, Foxp3(+)-, IL-10- and TGF-beta- independent, immunoregulatory effects.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro",
pages = "1347",
volume = "10",
doi = "10.1038/s41598-020-58177-2"
}

Soković Bajić, S., Đokić, J., Dinić, M., Tomić, S., Popović, N., Brdarić, E., Golić, N.,& Tolinački, M.. (2020). GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro. in Scientific Reports
Nature Publishing Group, London., 10, 1347.
https://doi.org/10.1038/s41598-020-58177-2

Soković Bajić S, Đokić J, Dinić M, Tomić S, Popović N, Brdarić E, Golić N, Tolinački M. GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro. in Scientific Reports. 2020;10:1347.
doi:10.1038/s41598-020-58177-2 .

Soković Bajić, Svetlana, Đokić, Jelena, Dinić, Miroslav, Tomić, Sergej, Popović, Nikola, Brdarić, Emilija, Golić, Nataša, Tolinački, Maja, "GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro" in Scientific Reports, 10 (2020):1347,
https://doi.org/10.1038/s41598-020-58177-2 . .

TY  - THES
AU  - Malešević, Milka
PY  - 2020
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=8012
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:23411/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=28784393
UR  - https://nardus.mpn.gov.rs/handle/123456789/18170
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/62
AB  - Nemogućnost kontrole pojave i širenja rezistencije na antibiotike usmerila je istraživanja u poslednje dve dekade ka iznalaženju novih terapeutskih opcija, sa ciljem tretmana infekcija izazvanih patogenim bakterijama rezistentnim na veći broj kliniĉki znaĉajnih antibiotika. Uzevši u obzir ĉinjenicu da je antibiotska rezistencija kompleksan, multifaktorijalni fenomen, rešenje ovog problema ukljuĉuje niz pristupa usmerenih na kontrolu faktora koji olakšavaju nastanak i širenje rezistencije. Jedan od tih pristupa sastoji se u razvoju novih terapeutika koji bi delovali mehanizmima razliĉitim od trenutno dostupnih antibiotika. U tom pogledu, antivirulentna terapija zamišljena je kao obećavajuća alternativa sa ciljem kontrole virulencije specifiĉne za odreĊene patogene, bez vršenja snažnog selektivnog pritiska na bakterijske ćelije. Imajući u vidu da je Pseudomonas aeruginosa jedan od vodećih uzroĉnika unutarbolniĉkih infekcija širom sveta, leĉenje infekcija izazvanih ovim patogenom predstavlja veliki terapeutski izazov. P. aeruginosa svoj patogeni potencijal ostvaruje zahvaljujući mnogobrojnim uroĊenim, steĉenim i adaptivnim mehanizmima rezistencije. Pored toga, posedovanje sistema meĊućelijske komunikacije (eng. quorum sensing, QS) ovom patogenu omogućava fleksibilnost u regulaciji ekspresije gena ukljuĉenih u virulenciju, formiranje biofilma, produkciju sekundarnih metabolita i faktora koji imaju ulogu u zaštiti od imunskog sistema domaćina. Stoga bi primena antivirulentne terapije zasnovane na utišavanju meĊućelijske komunikacije bakterija mogla poslužiti kao obećavajuće oruĊe u kontroli infekcija izazvanih predstavnicima P. aeruginosa za koje ne postoji adekvatna terapija trenutno dostupnim antibioticima. U skladu sa time, predmet prouĉavanja ove teze bila je potraga za novim utišivaĉima meĊućelijske komunikacije bakterija (eng. quorum quenching, QQ) produkovanih od strane kliniĉkih izolata koji tokom infekcija dele istu ekološku nišu sa P. aeruginosa. Analizirana je kolekcija Gramnegativnih kliniĉkih izolata Laboratorije za molekularnu mikrobiologiju, Instituta za molekularnu genetiku i genetiĉko inženjerstvo, Univerziteta u Beogradu sa ciljem pronalaženja sojeva koji produkuju QQ molekule. Nakon odabira sojeva nosioca QQ fenotipa, usledila je njihova identifikacija kao i funkcionalna karakterizacija na model sistemu kliniĉkog izolata P. aeruginosa MMA83. Upotrebom Chromobacterium violaceum CV026 biosenzora, selektovano je 19 sojeva (od ukupno 633 analiziranih izolata) koji su ispoljavali QQ fenotip, od kojih su dva izolata Delftia sp. 11304 i Burkholderia sp. BCC4135, na osnovu najznaĉajnije QQ aktivnosti izdvojena za detaljniju karakterizaciju. UtvrĊeno je da Delftia sp. 11304 soj pripada Delftia tsuruhatensis vrsti, dok Burkholderia sp. BCC4135 pripada Burkholderia cepacia vrsti sa novim sekvencnim tipom ST1485. In silico analizom genomskih sekvenci ustanovljeno je da oba odabrana soja poseduju izuzetan potencijal virulencije i rezistencije na antimikrobna jedinjenja. Priroda QQ molekula koje produkuju ovi sojevi bila je razliĉita; konstatovano je da soj 11304 produkuje male molekule neproteinske prirode (QS inhibitore, QSI), dok BCC4135 produkuje QQ enzime. Pored toga, kod BCC4135 izolata utvrĊeno je prisustvo i QS fenomena. Ukupni etil-acetatni ekstrakt supernatanta soja D. tsuruhatensis 11304 (QSI ekstrakt) ostvario je znaĉajan antivirulentni potencijal na model sistemu kliniĉkog izolata P. aeruginosa MMA83...
AB  - finding novel therapeutic options to combat multidrug-resistant pathogenic bacteria during the last two decades. Given the fact that antimicrobial resistance is a complex, multifactorial phenomenon, the solution to this problem comprises a range of approaches focused on monitoring the factors that facilitate the emergence and spread of resistance. One of proposed strategies consists of developing novel therapeutics that operate under different principles to the currently available antibiotics. In this respect, antivirulence therapy has been conceived as a promising alternative to control virulence in a pathogen-specific manner, without exerting strong selective pressure on the bacterial cells. Having in mind that Pseudomonas aeruginosa has been considered as a leading cause of nosocomial infections worldwide, the treatment of infections caused by this pathogen represents a major therapeutic challenge. The pathogenic potential of P. aeruginosa has been accomplished due to a numerous innate, acquired, and adaptive resistance mechanisms. In addition, the presence of cell-to-cell communication system (quorum sensing, QS) allows this pathogen the flexibility in the regulation of virulence gene expression responsible for biofilm development, production of secondary metabolites, and immuneevasive factors. Therefore, the use of antivirulence therapy based on the silencing of bacterial communication could serve as a promising tool in the control of infections caused by P. aeruginosa for which there is no adequate therapy with currently available antibiotics. Accordingly, the subject of this thesis was discovery and characterization of novel quenchers of bacterial cell-to-cell communication produced by clinical isolates that share the same ecological niche with P. aeruginosa during infections. A collection of Gram-negative clinical isolates from the Laboratory for Molecular Microbiology Institute of Molecular Genetics and Genetic Engineering University of Belgrade was analyzed in order to select the strains that produce quorum quenching (QQ) molecules. After the selection of strains carrying QQ phenotype, the identification of QQ molecules as well as their functional characterization on the model system of P. aeruginosa MMA83 clinical isolate was performed. Using Chromobacterium violaceum CV026 biosensor, 19 strains (out of a total of 633 analyzed isolates) with QQ phenotype were discovered, among which two clinical isolates Delftia sp. 11304 and Burkholderia sp. BCC4135 have been selected as strongest producers of QQ molecules for detailed characterization. According to the genomic sequence analysis, Delftia sp. 11304 was identified as a Delftia tsuruhatensis species while Burkholderia sp. BCC4135 was unveiled as Burkholderia cepacia with novel sequence type ST1485. In silico analysis of genomic sequences indicated that both selected strains possess significant antimicrobial resistance and virulence potential. The nature of the QQ molecules produced by these strains was determined as different; strain 11304 was found to produce small molecules of non-proteinaceous nature (QS inhibitors, QSI), while BCC4135 produced QQ enzymes. Besides, the presence of the QS phenomenon was determined in BCC4135 isolate. The total ethyl acetate extract of the D. tsuruhatensis 11304 culture supernatant (QSI extract) showed significant antivirulence potential on the model system of clinical isolate P. aeruginosa MMA83...
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Identifikacija i karakterizacija biogenih utišivača međućelijske komunikacije vrste Pseudomonas aeruginosa
T1  - Identification and characterization of biogenic silencers of Pseudomonas aeruginosa quorum sensing
UR  - https://hdl.handle.net/21.15107/rcub_nardus_18170
ER  - 

@phdthesis{
author = "Malešević, Milka",
year = "2020",
abstract = "Nemogućnost kontrole pojave i širenja rezistencije na antibiotike usmerila je istraživanja u poslednje dve dekade ka iznalaženju novih terapeutskih opcija, sa ciljem tretmana infekcija izazvanih patogenim bakterijama rezistentnim na veći broj kliniĉki znaĉajnih antibiotika. Uzevši u obzir ĉinjenicu da je antibiotska rezistencija kompleksan, multifaktorijalni fenomen, rešenje ovog problema ukljuĉuje niz pristupa usmerenih na kontrolu faktora koji olakšavaju nastanak i širenje rezistencije. Jedan od tih pristupa sastoji se u razvoju novih terapeutika koji bi delovali mehanizmima razliĉitim od trenutno dostupnih antibiotika. U tom pogledu, antivirulentna terapija zamišljena je kao obećavajuća alternativa sa ciljem kontrole virulencije specifiĉne za odreĊene patogene, bez vršenja snažnog selektivnog pritiska na bakterijske ćelije. Imajući u vidu da je Pseudomonas aeruginosa jedan od vodećih uzroĉnika unutarbolniĉkih infekcija širom sveta, leĉenje infekcija izazvanih ovim patogenom predstavlja veliki terapeutski izazov. P. aeruginosa svoj patogeni potencijal ostvaruje zahvaljujući mnogobrojnim uroĊenim, steĉenim i adaptivnim mehanizmima rezistencije. Pored toga, posedovanje sistema meĊućelijske komunikacije (eng. quorum sensing, QS) ovom patogenu omogućava fleksibilnost u regulaciji ekspresije gena ukljuĉenih u virulenciju, formiranje biofilma, produkciju sekundarnih metabolita i faktora koji imaju ulogu u zaštiti od imunskog sistema domaćina. Stoga bi primena antivirulentne terapije zasnovane na utišavanju meĊućelijske komunikacije bakterija mogla poslužiti kao obećavajuće oruĊe u kontroli infekcija izazvanih predstavnicima P. aeruginosa za koje ne postoji adekvatna terapija trenutno dostupnim antibioticima. U skladu sa time, predmet prouĉavanja ove teze bila je potraga za novim utišivaĉima meĊućelijske komunikacije bakterija (eng. quorum quenching, QQ) produkovanih od strane kliniĉkih izolata koji tokom infekcija dele istu ekološku nišu sa P. aeruginosa. Analizirana je kolekcija Gramnegativnih kliniĉkih izolata Laboratorije za molekularnu mikrobiologiju, Instituta za molekularnu genetiku i genetiĉko inženjerstvo, Univerziteta u Beogradu sa ciljem pronalaženja sojeva koji produkuju QQ molekule. Nakon odabira sojeva nosioca QQ fenotipa, usledila je njihova identifikacija kao i funkcionalna karakterizacija na model sistemu kliniĉkog izolata P. aeruginosa MMA83. Upotrebom Chromobacterium violaceum CV026 biosenzora, selektovano je 19 sojeva (od ukupno 633 analiziranih izolata) koji su ispoljavali QQ fenotip, od kojih su dva izolata Delftia sp. 11304 i Burkholderia sp. BCC4135, na osnovu najznaĉajnije QQ aktivnosti izdvojena za detaljniju karakterizaciju. UtvrĊeno je da Delftia sp. 11304 soj pripada Delftia tsuruhatensis vrsti, dok Burkholderia sp. BCC4135 pripada Burkholderia cepacia vrsti sa novim sekvencnim tipom ST1485. In silico analizom genomskih sekvenci ustanovljeno je da oba odabrana soja poseduju izuzetan potencijal virulencije i rezistencije na antimikrobna jedinjenja. Priroda QQ molekula koje produkuju ovi sojevi bila je razliĉita; konstatovano je da soj 11304 produkuje male molekule neproteinske prirode (QS inhibitore, QSI), dok BCC4135 produkuje QQ enzime. Pored toga, kod BCC4135 izolata utvrĊeno je prisustvo i QS fenomena. Ukupni etil-acetatni ekstrakt supernatanta soja D. tsuruhatensis 11304 (QSI ekstrakt) ostvario je znaĉajan antivirulentni potencijal na model sistemu kliniĉkog izolata P. aeruginosa MMA83..., finding novel therapeutic options to combat multidrug-resistant pathogenic bacteria during the last two decades. Given the fact that antimicrobial resistance is a complex, multifactorial phenomenon, the solution to this problem comprises a range of approaches focused on monitoring the factors that facilitate the emergence and spread of resistance. One of proposed strategies consists of developing novel therapeutics that operate under different principles to the currently available antibiotics. In this respect, antivirulence therapy has been conceived as a promising alternative to control virulence in a pathogen-specific manner, without exerting strong selective pressure on the bacterial cells. Having in mind that Pseudomonas aeruginosa has been considered as a leading cause of nosocomial infections worldwide, the treatment of infections caused by this pathogen represents a major therapeutic challenge. The pathogenic potential of P. aeruginosa has been accomplished due to a numerous innate, acquired, and adaptive resistance mechanisms. In addition, the presence of cell-to-cell communication system (quorum sensing, QS) allows this pathogen the flexibility in the regulation of virulence gene expression responsible for biofilm development, production of secondary metabolites, and immuneevasive factors. Therefore, the use of antivirulence therapy based on the silencing of bacterial communication could serve as a promising tool in the control of infections caused by P. aeruginosa for which there is no adequate therapy with currently available antibiotics. Accordingly, the subject of this thesis was discovery and characterization of novel quenchers of bacterial cell-to-cell communication produced by clinical isolates that share the same ecological niche with P. aeruginosa during infections. A collection of Gram-negative clinical isolates from the Laboratory for Molecular Microbiology Institute of Molecular Genetics and Genetic Engineering University of Belgrade was analyzed in order to select the strains that produce quorum quenching (QQ) molecules. After the selection of strains carrying QQ phenotype, the identification of QQ molecules as well as their functional characterization on the model system of P. aeruginosa MMA83 clinical isolate was performed. Using Chromobacterium violaceum CV026 biosensor, 19 strains (out of a total of 633 analyzed isolates) with QQ phenotype were discovered, among which two clinical isolates Delftia sp. 11304 and Burkholderia sp. BCC4135 have been selected as strongest producers of QQ molecules for detailed characterization. According to the genomic sequence analysis, Delftia sp. 11304 was identified as a Delftia tsuruhatensis species while Burkholderia sp. BCC4135 was unveiled as Burkholderia cepacia with novel sequence type ST1485. In silico analysis of genomic sequences indicated that both selected strains possess significant antimicrobial resistance and virulence potential. The nature of the QQ molecules produced by these strains was determined as different; strain 11304 was found to produce small molecules of non-proteinaceous nature (QS inhibitors, QSI), while BCC4135 produced QQ enzymes. Besides, the presence of the QS phenomenon was determined in BCC4135 isolate. The total ethyl acetate extract of the D. tsuruhatensis 11304 culture supernatant (QSI extract) showed significant antivirulence potential on the model system of clinical isolate P. aeruginosa MMA83...",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Identifikacija i karakterizacija biogenih utišivača međućelijske komunikacije vrste Pseudomonas aeruginosa, Identification and characterization of biogenic silencers of Pseudomonas aeruginosa quorum sensing",
url = "https://hdl.handle.net/21.15107/rcub_nardus_18170"
}

Malešević, M.. (2020). Identifikacija i karakterizacija biogenih utišivača međućelijske komunikacije vrste Pseudomonas aeruginosa. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_18170

Malešević M. Identifikacija i karakterizacija biogenih utišivača međućelijske komunikacije vrste Pseudomonas aeruginosa. 2020;.
https://hdl.handle.net/21.15107/rcub_nardus_18170 .

Malešević, Milka, "Identifikacija i karakterizacija biogenih utišivača međućelijske komunikacije vrste Pseudomonas aeruginosa" (2020),
https://hdl.handle.net/21.15107/rcub_nardus_18170 .

TY  - JOUR
AU  - Milincić, Danijel D.
AU  - Vojinović, Uros D.
AU  - Kostić, Aleksandar Z.
AU  - Pesić, Mirjana B.
AU  - Spirović-Trifunović, Bojana D.
AU  - Brkić, Dragica, V
AU  - Stević, Milan Z.
AU  - Kojić, Milan
AU  - Stanisavljević, Nemanja
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1319
AB  - The aim of this study was to investigate the bioaccessibility of pesticide residues in blueberries (com-mercial and sample from controlled field trial) from Serbia, involving the presence of a complex food matrix and to assess the potential risk to human health. The presence of nine active substances (azox-ystrobin, boscalid, fludioxonil, cyprodinil, pyrimethanil, pyridaben, pyriproxyfen, acetamiprid and thia-metoxam) in initial blueberry samples was determined in concentration range from 5.15 mu g/kg for thiametoxam to 187 mu g/kg for azoxystrobin. Clothianidin, metabolite of thiametoxam, was not detected in any blueberry sample. However, after in vitro digestion, the content of initially detected pesticides residues was significantly decreased or it was below limit of quantification resulting in the total bio-accessibility of about 15%. Azoxystrobin, pyrimethanil and fludioxonil was quantified in digestive juice at concentrations which were about 81%, 37% and 10% less than the inital concentration, respectively. The presence of food matrix during digestion of blueberries even more severely reduced concentration of pesticide residues (total bioaccessibility was about 7%) compared to digestion without the food matrix. Only azoxystrobin was quantified after digestion with food matrix in concentration of 27 mu g/kg in sample from controlled field trial and detected in two commercial samples but below the limit of quantification. Furthermore, chronic risk assessment indicated that risk is acceptable for the health of different human subpopulation groups. The current study on pesticides residues, most commonly applied on blueberries, provides for the first time an insight into their bioaccessibility under conditions that mimic physiological environment of human digestive tract.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Chemosphere
T1  - In vitro assessment of pesticide residues bioaccessibility in conventionally grown blueberries as affected by complex food matrix
VL  - 252
DO  - 10.1016/j.chemosphere.2020.126568
ER  - 

@article{
author = "Milincić, Danijel D. and Vojinović, Uros D. and Kostić, Aleksandar Z. and Pesić, Mirjana B. and Spirović-Trifunović, Bojana D. and Brkić, Dragica, V and Stević, Milan Z. and Kojić, Milan and Stanisavljević, Nemanja",
year = "2020",
abstract = "The aim of this study was to investigate the bioaccessibility of pesticide residues in blueberries (com-mercial and sample from controlled field trial) from Serbia, involving the presence of a complex food matrix and to assess the potential risk to human health. The presence of nine active substances (azox-ystrobin, boscalid, fludioxonil, cyprodinil, pyrimethanil, pyridaben, pyriproxyfen, acetamiprid and thia-metoxam) in initial blueberry samples was determined in concentration range from 5.15 mu g/kg for thiametoxam to 187 mu g/kg for azoxystrobin. Clothianidin, metabolite of thiametoxam, was not detected in any blueberry sample. However, after in vitro digestion, the content of initially detected pesticides residues was significantly decreased or it was below limit of quantification resulting in the total bio-accessibility of about 15%. Azoxystrobin, pyrimethanil and fludioxonil was quantified in digestive juice at concentrations which were about 81%, 37% and 10% less than the inital concentration, respectively. The presence of food matrix during digestion of blueberries even more severely reduced concentration of pesticide residues (total bioaccessibility was about 7%) compared to digestion without the food matrix. Only azoxystrobin was quantified after digestion with food matrix in concentration of 27 mu g/kg in sample from controlled field trial and detected in two commercial samples but below the limit of quantification. Furthermore, chronic risk assessment indicated that risk is acceptable for the health of different human subpopulation groups. The current study on pesticides residues, most commonly applied on blueberries, provides for the first time an insight into their bioaccessibility under conditions that mimic physiological environment of human digestive tract.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Chemosphere",
title = "In vitro assessment of pesticide residues bioaccessibility in conventionally grown blueberries as affected by complex food matrix",
volume = "252",
doi = "10.1016/j.chemosphere.2020.126568"
}

Milincić, D. D., Vojinović, U. D., Kostić, A. Z., Pesić, M. B., Spirović-Trifunović, B. D., Brkić, D. V., Stević, M. Z., Kojić, M.,& Stanisavljević, N.. (2020). In vitro assessment of pesticide residues bioaccessibility in conventionally grown blueberries as affected by complex food matrix. in Chemosphere
Pergamon-Elsevier Science Ltd, Oxford., 252.
https://doi.org/10.1016/j.chemosphere.2020.126568

Milincić DD, Vojinović UD, Kostić AZ, Pesić MB, Spirović-Trifunović BD, Brkić DV, Stević MZ, Kojić M, Stanisavljević N. In vitro assessment of pesticide residues bioaccessibility in conventionally grown blueberries as affected by complex food matrix. in Chemosphere. 2020;252.
doi:10.1016/j.chemosphere.2020.126568 .

Milincić, Danijel D., Vojinović, Uros D., Kostić, Aleksandar Z., Pesić, Mirjana B., Spirović-Trifunović, Bojana D., Brkić, Dragica, V, Stević, Milan Z., Kojić, Milan, Stanisavljević, Nemanja, "In vitro assessment of pesticide residues bioaccessibility in conventionally grown blueberries as affected by complex food matrix" in Chemosphere, 252 (2020),
https://doi.org/10.1016/j.chemosphere.2020.126568 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Kosanović, Dejana
AU  - Terzić-Vidojević, Amarela
AU  - Begović, Jelena
AU  - Golić, Nataša
AU  - Jeney, Galina
AU  - Ljubobratović, Uros
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1382
AB  - This study aimed to analyze an impact of Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 supplemented to Anemia franciscana cultivation medium on biochemical profile of hatched nauplii. Impacts of different BGHO1:BGGO6-55 ratios (75:25, 50:50 and 25:75) and their total concentrations (250 and 500 g/kg of Anemia cysts) at different timings of bacteria application (pre- and post-hatching) were examined. The effects were evaluated by quantification of naupliar peptide, soluble protein, phospho-, neutral and unsaturated lipid content. Uni and multifactorial analysis of variance (ANOVA) were used to estimate the effects of treatments relative to control and to model factor interactions, respectively Statistical analysis indicated that posthatching application of 75:25 strain ratio at high dose was associated with an increase of neutral lipid amount. Furthermore, factor interaction profiling identified positive correlation of lacto-bacilli concentration with the level of free amino acids/short peptides, phospho-, neutral and unsaturated lipids, but only at 50:50 strain ratio combination. Application of lower lactobacilli dose at 75:25 strain ratio caused an increase of soluble protein and phospholipid amount. Hypothetically, graded response of Artemia nauplii to lactobacilli supplementation was induced with different BGHO1 doses. It assumedly ranged from stress-response protein synthesis at lower doses to membrane permeability alterations and triglyceride-mediated defense mechanism activation at higher BGHO1 doses. In contrast to lactobacilli supplementation after Anemia hatching, pre-hatching application of lactobacilli was not associated with an increase of Anemia nutritive profile, though again there was a positive association of lactobacilli concentration with nutrient amount. This implicates interference of lactobacilli with the hatching process. Results presented here facilitate the design of future studies aiming to modify Artemia nutritive profile in accordance with nutritional demands of cultivated fish species.
PB  - Elsevier, Amsterdam
T2  - Animal Feed Science and Technology
T1  - Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner
VL  - 259
DO  - 10.1016/j.anifeedsci.2019.114356
ER  - 

@article{
author = "Lukić, Jovanka and Stanisavljević, Nemanja and Vukotić, Goran and Kosanović, Dejana and Terzić-Vidojević, Amarela and Begović, Jelena and Golić, Nataša and Jeney, Galina and Ljubobratović, Uros",
year = "2020",
abstract = "This study aimed to analyze an impact of Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 supplemented to Anemia franciscana cultivation medium on biochemical profile of hatched nauplii. Impacts of different BGHO1:BGGO6-55 ratios (75:25, 50:50 and 25:75) and their total concentrations (250 and 500 g/kg of Anemia cysts) at different timings of bacteria application (pre- and post-hatching) were examined. The effects were evaluated by quantification of naupliar peptide, soluble protein, phospho-, neutral and unsaturated lipid content. Uni and multifactorial analysis of variance (ANOVA) were used to estimate the effects of treatments relative to control and to model factor interactions, respectively Statistical analysis indicated that posthatching application of 75:25 strain ratio at high dose was associated with an increase of neutral lipid amount. Furthermore, factor interaction profiling identified positive correlation of lacto-bacilli concentration with the level of free amino acids/short peptides, phospho-, neutral and unsaturated lipids, but only at 50:50 strain ratio combination. Application of lower lactobacilli dose at 75:25 strain ratio caused an increase of soluble protein and phospholipid amount. Hypothetically, graded response of Artemia nauplii to lactobacilli supplementation was induced with different BGHO1 doses. It assumedly ranged from stress-response protein synthesis at lower doses to membrane permeability alterations and triglyceride-mediated defense mechanism activation at higher BGHO1 doses. In contrast to lactobacilli supplementation after Anemia hatching, pre-hatching application of lactobacilli was not associated with an increase of Anemia nutritive profile, though again there was a positive association of lactobacilli concentration with nutrient amount. This implicates interference of lactobacilli with the hatching process. Results presented here facilitate the design of future studies aiming to modify Artemia nutritive profile in accordance with nutritional demands of cultivated fish species.",
publisher = "Elsevier, Amsterdam",
journal = "Animal Feed Science and Technology",
title = "Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner",
volume = "259",
doi = "10.1016/j.anifeedsci.2019.114356"
}

Lukić, J., Stanisavljević, N., Vukotić, G., Kosanović, D., Terzić-Vidojević, A., Begović, J., Golić, N., Jeney, G.,& Ljubobratović, U.. (2020). Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner. in Animal Feed Science and Technology
Elsevier, Amsterdam., 259.
https://doi.org/10.1016/j.anifeedsci.2019.114356

Lukić J, Stanisavljević N, Vukotić G, Kosanović D, Terzić-Vidojević A, Begović J, Golić N, Jeney G, Ljubobratović U. Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner. in Animal Feed Science and Technology. 2020;259.
doi:10.1016/j.anifeedsci.2019.114356 .

Lukić, Jovanka, Stanisavljević, Nemanja, Vukotić, Goran, Kosanović, Dejana, Terzić-Vidojević, Amarela, Begović, Jelena, Golić, Nataša, Jeney, Galina, Ljubobratović, Uros, "Lactobacillus salivarius BGHO1 and Lactobacillus reuteri BGGO6-55 modify nutritive profile of Artemia franciscana nauplii in a strain ratio, dose and application timing-dependent manner" in Animal Feed Science and Technology, 259 (2020),
https://doi.org/10.1016/j.anifeedsci.2019.114356 . .

TY  - JOUR
AU  - Ljubobratović, Uros
AU  - Kosanović, Dejana
AU  - Demeny, Ferenc Zoltan
AU  - Krajcsovics, Adrienn
AU  - Vukotić, Goran
AU  - Stanisavljević, Nemanja
AU  - Golić, Nataša
AU  - Jeney, Galina
AU  - Lukić, Jovanka
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1402
AB  - Weaning to inert diet in intensively reared pike-perch larvae is confronted with significant fish loss, which prevents successful commercialization of pike-perch production. Achievement of satisfactory feed quality and effective assimilation of nutrients by larval fish is the major challenge in larval production process. Aim of this study was to evaluate whether treatment of live and inert feed with lactobacilli could alleviate growth retardation associated with early weaning of pike-perch reared in recirculating system. Weaning started on 18th day post-hatching (DPH) either as sudden weaning (SW) or by co-administration of Anemia for six days (gradual weaning, GW). Prior to administration to fish, Anemia was treated with Lactobacillus salivarius BGHO1/Lb. reuteri BGGO6-55, while inert feed was treated with Lb. paracasei subsp. paracasei BGHN14/Lb. rhamnosus BGT10. Treatment with lactobacilli slightly raised neutral lipid level in Anemia nauplii, but significantly reduced their content in dry feed. Fish were sampled on the 24th DPH. Survival, morphometric indices, skeleton differentiation, digestive enzyme activity and opportunistic pathogenic bacteria level were assessed in whole fish specimens. GW fish were presented with better survival, body growth and phospholipase A2 (PLA2) activity. Alongside, Vibrio spp. growth was suppressed in these fish and skeleton development was improved, according to Alizarin Red staining and ColIA1/Sparc mRNA expression data. Lactobacilli application in GW fish correlated with an increase of survival, condition factor and growth rate, according to trypsin and chymotrypsin activities, indicating better utilization of dietary proteins for muscle building. In SW fish, lactobacilli elevated chymotrypsin activity, PLA2 to lipase activity ratio and improved survival and ossification, as evident from Alizarin Red staining and ColIA1/Sparc mRNA expression. This indicated improved fatty acid absorption and control of metamorphosis process. Furthermore, lactobacilli suppressed Vibrio spp. growth in SW fish. Aside from demonstrating the ability of lactobacilli to aid weaning in pike-perch larvae, this study indicated that different types of food treatment may direct fish growth in a predictable manner, allowing further cost-effective improvements of larval pike-perch rearing in intensive system.
PB  - Elsevier, Amsterdam
T2  - Aquaculture
T1  - The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae
VL  - 516
DO  - 10.1016/j.aquaculture.2019.734608
ER  - 

@article{
author = "Ljubobratović, Uros and Kosanović, Dejana and Demeny, Ferenc Zoltan and Krajcsovics, Adrienn and Vukotić, Goran and Stanisavljević, Nemanja and Golić, Nataša and Jeney, Galina and Lukić, Jovanka",
year = "2020",
abstract = "Weaning to inert diet in intensively reared pike-perch larvae is confronted with significant fish loss, which prevents successful commercialization of pike-perch production. Achievement of satisfactory feed quality and effective assimilation of nutrients by larval fish is the major challenge in larval production process. Aim of this study was to evaluate whether treatment of live and inert feed with lactobacilli could alleviate growth retardation associated with early weaning of pike-perch reared in recirculating system. Weaning started on 18th day post-hatching (DPH) either as sudden weaning (SW) or by co-administration of Anemia for six days (gradual weaning, GW). Prior to administration to fish, Anemia was treated with Lactobacillus salivarius BGHO1/Lb. reuteri BGGO6-55, while inert feed was treated with Lb. paracasei subsp. paracasei BGHN14/Lb. rhamnosus BGT10. Treatment with lactobacilli slightly raised neutral lipid level in Anemia nauplii, but significantly reduced their content in dry feed. Fish were sampled on the 24th DPH. Survival, morphometric indices, skeleton differentiation, digestive enzyme activity and opportunistic pathogenic bacteria level were assessed in whole fish specimens. GW fish were presented with better survival, body growth and phospholipase A2 (PLA2) activity. Alongside, Vibrio spp. growth was suppressed in these fish and skeleton development was improved, according to Alizarin Red staining and ColIA1/Sparc mRNA expression data. Lactobacilli application in GW fish correlated with an increase of survival, condition factor and growth rate, according to trypsin and chymotrypsin activities, indicating better utilization of dietary proteins for muscle building. In SW fish, lactobacilli elevated chymotrypsin activity, PLA2 to lipase activity ratio and improved survival and ossification, as evident from Alizarin Red staining and ColIA1/Sparc mRNA expression. This indicated improved fatty acid absorption and control of metamorphosis process. Furthermore, lactobacilli suppressed Vibrio spp. growth in SW fish. Aside from demonstrating the ability of lactobacilli to aid weaning in pike-perch larvae, this study indicated that different types of food treatment may direct fish growth in a predictable manner, allowing further cost-effective improvements of larval pike-perch rearing in intensive system.",
publisher = "Elsevier, Amsterdam",
journal = "Aquaculture",
title = "The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae",
volume = "516",
doi = "10.1016/j.aquaculture.2019.734608"
}

Ljubobratović, U., Kosanović, D., Demeny, F. Z., Krajcsovics, A., Vukotić, G., Stanisavljević, N., Golić, N., Jeney, G.,& Lukić, J.. (2020). The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae. in Aquaculture
Elsevier, Amsterdam., 516.
https://doi.org/10.1016/j.aquaculture.2019.734608

Ljubobratović U, Kosanović D, Demeny FZ, Krajcsovics A, Vukotić G, Stanisavljević N, Golić N, Jeney G, Lukić J. The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae. in Aquaculture. 2020;516.
doi:10.1016/j.aquaculture.2019.734608 .

Ljubobratović, Uros, Kosanović, Dejana, Demeny, Ferenc Zoltan, Krajcsovics, Adrienn, Vukotić, Goran, Stanisavljević, Nemanja, Golić, Nataša, Jeney, Galina, Lukić, Jovanka, "The effect of live and inert feed treatment with lactobacilli on weaning success in intensively reared pike-perch larvae" in Aquaculture, 516 (2020),
https://doi.org/10.1016/j.aquaculture.2019.734608 . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Živković, Milica
AU  - Lukić, Jovanka
AU  - Lozo, Jelena
AU  - Fira, Đorđe
AU  - Jovčić, Branko
AU  - Strahinić, Ivana
AU  - Begović, Jelena
AU  - Popović, Nikola
AU  - Miljković, Marija
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
AU  - Golić, Nataša
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1353
AB  - The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.
PB  - Elsevier, Amsterdam
T2  - Food Research International
T1  - Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties
VL  - 136
DO  - 10.1016/j.foodres.2020.109494
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Tolinački, Maja and Živković, Milica and Lukić, Jovanka and Lozo, Jelena and Fira, Đorđe and Jovčić, Branko and Strahinić, Ivana and Begović, Jelena and Popović, Nikola and Miljković, Marija and Kojić, Milan and Topisirović, Ljubiša and Golić, Nataša",
year = "2020",
abstract = "The aim of this review was to summarize the data regarding diversity of non-starter lactic acid bacteria (NSLAB) isolated from various artisanal dairy products manufactured in Western Balkan Countries. The dairy products examined were manufactured from raw cow's, sheep's or goat's milk or mixed milk, in the traditional way without the addition of commercial starter cultures. Dairy products such as white brined cheese, fresh cheese, hard cheese, yogurt, sour cream and kajmak were sampled in the households of Serbia, Croatia, Slovenia, Bosnia and Herzegovina, Montenegro, and North Macedonia. It has been established that the diversity of lactic acid bacteria (LAB) from raw milk artisanal dairy products is extensive. In the reviewed literature, 28 LAB species and a large number of strains belonging to the Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Pediococcus, Leuconostoc and Weissella genera were isolated from various dairy products. Over 3000 LAB strains were obtained and characterized for their technological and probiotic properties including: acidification and coagulation of milk, production of aromatic compounds, proteolytic activity, bacteriocins production and competitive exclusion of pathogens, production of exopolysaccharides, aggregation ability and immunomodulatory effect. Results show that many of the isolated NSLAB strains had one, two or more of the properties mentioned. The data presented emphasize the importance of artisanal products as a valuable source of NSLAB with unique technological and probiotic features important both as a base for scientific research as well as for designing novel starter cultures for functional dairy food.",
publisher = "Elsevier, Amsterdam",
journal = "Food Research International",
title = "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties",
volume = "136",
doi = "10.1016/j.foodres.2020.109494"
}

Terzić-Vidojević, A., Veljović, K., Tolinački, M., Živković, M., Lukić, J., Lozo, J., Fira, Đ., Jovčić, B., Strahinić, I., Begović, J., Popović, N., Miljković, M., Kojić, M., Topisirović, L.,& Golić, N.. (2020). Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International
Elsevier, Amsterdam., 136.
https://doi.org/10.1016/j.foodres.2020.109494

Terzić-Vidojević A, Veljović K, Tolinački M, Živković M, Lukić J, Lozo J, Fira Đ, Jovčić B, Strahinić I, Begović J, Popović N, Miljković M, Kojić M, Topisirović L, Golić N. Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties. in Food Research International. 2020;136.
doi:10.1016/j.foodres.2020.109494 .

Terzić-Vidojević, Amarela, Veljović, Katarina, Tolinački, Maja, Živković, Milica, Lukić, Jovanka, Lozo, Jelena, Fira, Đorđe, Jovčić, Branko, Strahinić, Ivana, Begović, Jelena, Popović, Nikola, Miljković, Marija, Kojić, Milan, Topisirović, Ljubiša, Golić, Nataša, "Diversity of non-starter lactic acid bacteria in autochthonous dairy products from Western Balkan Countries - Technological and probiotic properties" in Food Research International, 136 (2020),
https://doi.org/10.1016/j.foodres.2020.109494 . .

TY  - JOUR
AU  - Mirković, Nemanja
AU  - Kulas, Jelena
AU  - Miloradović, Zorana
AU  - Miljković, Marija
AU  - Tucović, Dina
AU  - Miocinović, Jelena
AU  - Jovčić, Branko
AU  - Mirkov, Ivana
AU  - Kojić, Milan
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1388
AB  - In last two decades, there has been a strong trend in the application of lactic acid bacteria as adjunctive cultures to control growth of spoilage and pathogenic bacteria in food. One of the most important properties that contribute to the application of these bacteria is the production of antimicrobial molecules. Lactococcus lactis subsp. lactis BGBU1-4, isolated from traditional brined cheese, produces thermostable bacteriocin named lactolisterin BU, with broad spectrum of activity against spoilage bacteria and foodborne pathogens. In this study, effect of strain BGBU1-4, as adjunct culture, on the numbers of Listeria monocytogenes ATCC19111 and Staphylococcus aureus LMM322 in artificially contaminated Quark-type, soft acid coagulated cheese, was examined. In addition, we analyzed influence of BGBU1-4 on chemical and sensory properties of the cheese, as well as immunological response of Albino oxford rats fed with Quark-type of cheese made using BGBU1-4 as adjunct culture. Results of this study revealed antibacterial potential of strain BGBU1-4 against L. monocytogenes ATCC19111 and S. aureus LMM322 in Quark-type cheese during 21 days of storage at 4 degrees C. Also, it was noticed the ability of BGBU1-4 to control the spontaneously grown yeasts and molds. Chemical composition and pH values of cheese containing BGBU1-4 were unchanged in comparison to control. The sensory quality scores showed that there was difference between cheese with and without adjunct culture in terms of flavor and oral texture, while for the odor and appearance no differences between two cheese variants were scored. Results of the immunological response of Albino rats fed with Quark-type cheese containing BGBU1-4 indicate absence of systematic inflammation. However, increased pro-inflammatory cytokines content (IL-1 beta, IL-6, IL-17) in intestine of rats fed with cheese containing BGBU1-4, concomitantly with unchanged anti-inflammatory cytokines suggests disruption of gut homeostasis and inflammation in this tissue. The changes caused by BGBU1-4 are reversible, system returns into homeostasis seven days after cessation of feeding with cheese containing BGBU1-4.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Control
T1  - Lactolisterin BU-producer Lactococcus lactis subsp. lactis BGBU1-4: Biocontrol of Listeria monocytogenes and Staphylocococcus aureus in fresh soft cheese and effect on immunological response of rats
VL  - 111
DO  - 10.1016/j.foodcont.2019.107076
ER  - 

@article{
author = "Mirković, Nemanja and Kulas, Jelena and Miloradović, Zorana and Miljković, Marija and Tucović, Dina and Miocinović, Jelena and Jovčić, Branko and Mirkov, Ivana and Kojić, Milan",
year = "2020",
abstract = "In last two decades, there has been a strong trend in the application of lactic acid bacteria as adjunctive cultures to control growth of spoilage and pathogenic bacteria in food. One of the most important properties that contribute to the application of these bacteria is the production of antimicrobial molecules. Lactococcus lactis subsp. lactis BGBU1-4, isolated from traditional brined cheese, produces thermostable bacteriocin named lactolisterin BU, with broad spectrum of activity against spoilage bacteria and foodborne pathogens. In this study, effect of strain BGBU1-4, as adjunct culture, on the numbers of Listeria monocytogenes ATCC19111 and Staphylococcus aureus LMM322 in artificially contaminated Quark-type, soft acid coagulated cheese, was examined. In addition, we analyzed influence of BGBU1-4 on chemical and sensory properties of the cheese, as well as immunological response of Albino oxford rats fed with Quark-type of cheese made using BGBU1-4 as adjunct culture. Results of this study revealed antibacterial potential of strain BGBU1-4 against L. monocytogenes ATCC19111 and S. aureus LMM322 in Quark-type cheese during 21 days of storage at 4 degrees C. Also, it was noticed the ability of BGBU1-4 to control the spontaneously grown yeasts and molds. Chemical composition and pH values of cheese containing BGBU1-4 were unchanged in comparison to control. The sensory quality scores showed that there was difference between cheese with and without adjunct culture in terms of flavor and oral texture, while for the odor and appearance no differences between two cheese variants were scored. Results of the immunological response of Albino rats fed with Quark-type cheese containing BGBU1-4 indicate absence of systematic inflammation. However, increased pro-inflammatory cytokines content (IL-1 beta, IL-6, IL-17) in intestine of rats fed with cheese containing BGBU1-4, concomitantly with unchanged anti-inflammatory cytokines suggests disruption of gut homeostasis and inflammation in this tissue. The changes caused by BGBU1-4 are reversible, system returns into homeostasis seven days after cessation of feeding with cheese containing BGBU1-4.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Control",
title = "Lactolisterin BU-producer Lactococcus lactis subsp. lactis BGBU1-4: Biocontrol of Listeria monocytogenes and Staphylocococcus aureus in fresh soft cheese and effect on immunological response of rats",
volume = "111",
doi = "10.1016/j.foodcont.2019.107076"
}

Mirković, N., Kulas, J., Miloradović, Z., Miljković, M., Tucović, D., Miocinović, J., Jovčić, B., Mirkov, I.,& Kojić, M.. (2020). Lactolisterin BU-producer Lactococcus lactis subsp. lactis BGBU1-4: Biocontrol of Listeria monocytogenes and Staphylocococcus aureus in fresh soft cheese and effect on immunological response of rats. in Food Control
Elsevier Sci Ltd, Oxford., 111.
https://doi.org/10.1016/j.foodcont.2019.107076

Mirković N, Kulas J, Miloradović Z, Miljković M, Tucović D, Miocinović J, Jovčić B, Mirkov I, Kojić M. Lactolisterin BU-producer Lactococcus lactis subsp. lactis BGBU1-4: Biocontrol of Listeria monocytogenes and Staphylocococcus aureus in fresh soft cheese and effect on immunological response of rats. in Food Control. 2020;111.
doi:10.1016/j.foodcont.2019.107076 .

Mirković, Nemanja, Kulas, Jelena, Miloradović, Zorana, Miljković, Marija, Tucović, Dina, Miocinović, Jelena, Jovčić, Branko, Mirkov, Ivana, Kojić, Milan, "Lactolisterin BU-producer Lactococcus lactis subsp. lactis BGBU1-4: Biocontrol of Listeria monocytogenes and Staphylocococcus aureus in fresh soft cheese and effect on immunological response of rats" in Food Control, 111 (2020),
https://doi.org/10.1016/j.foodcont.2019.107076 . .

TY  - JOUR
AU  - Klisurić, Olivera R.
AU  - Armaković, Sanja J.
AU  - Armaković, Stevan
AU  - Marković, Sanja
AU  - Todorović, Tamara R.
AU  - Portalone, Gustavo
AU  - Novović, Katarina
AU  - Lozo, Jelena
AU  - Filipović, Nenad R.
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1405
AB  - In this work pharmaceutical application of focused library of six quinoline-based chalcogensemicarbazones (QBCs) was tested through determination of their antimicrobial activity against twenty-eight Gram-negative and Gram-positive strains from different origin. Pharmacokinetic properties have been assessed by the analysis of frequently employed drug likeness parameters. Computational study has been complemented with calculation of their global and local reactive properties, within the framework of density functional theory (DFT). Among other information, DFT calculations helped us to locate the most reactive sites of investigated QBCs and to identify their sensitivity towards the oxidation.
PB  - Elsevier, Amsterdam
T2  - Journal of Molecular Structure
T1  - Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones
VL  - 1203
DO  - 10.1016/j.molstruc.2019.127482
ER  - 

@article{
author = "Klisurić, Olivera R. and Armaković, Sanja J. and Armaković, Stevan and Marković, Sanja and Todorović, Tamara R. and Portalone, Gustavo and Novović, Katarina and Lozo, Jelena and Filipović, Nenad R.",
year = "2020",
abstract = "In this work pharmaceutical application of focused library of six quinoline-based chalcogensemicarbazones (QBCs) was tested through determination of their antimicrobial activity against twenty-eight Gram-negative and Gram-positive strains from different origin. Pharmacokinetic properties have been assessed by the analysis of frequently employed drug likeness parameters. Computational study has been complemented with calculation of their global and local reactive properties, within the framework of density functional theory (DFT). Among other information, DFT calculations helped us to locate the most reactive sites of investigated QBCs and to identify their sensitivity towards the oxidation.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Molecular Structure",
title = "Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones",
volume = "1203",
doi = "10.1016/j.molstruc.2019.127482"
}

Klisurić, O. R., Armaković, S. J., Armaković, S., Marković, S., Todorović, T. R., Portalone, G., Novović, K., Lozo, J.,& Filipović, N. R.. (2020). Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones. in Journal of Molecular Structure
Elsevier, Amsterdam., 1203.
https://doi.org/10.1016/j.molstruc.2019.127482

Klisurić OR, Armaković SJ, Armaković S, Marković S, Todorović TR, Portalone G, Novović K, Lozo J, Filipović NR. Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones. in Journal of Molecular Structure. 2020;1203.
doi:10.1016/j.molstruc.2019.127482 .

Klisurić, Olivera R., Armaković, Sanja J., Armaković, Stevan, Marković, Sanja, Todorović, Tamara R., Portalone, Gustavo, Novović, Katarina, Lozo, Jelena, Filipović, Nenad R., "Structural, biological and in-silico study of quinoline-based chalcogensemicarbazones" in Journal of Molecular Structure, 1203 (2020),
https://doi.org/10.1016/j.molstruc.2019.127482 . .

TY  - JOUR
AU  - Tomović, Katarina
AU  - Ilić, Budimir S.
AU  - Smelcerović, Zaklina
AU  - Miljković, Marija
AU  - Yancheva, Denitsa
AU  - Kojić, Milan
AU  - Mavrova, Anelia Ts
AU  - Kocić, Gordana
AU  - Smelcerović, Andrija
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1376
AB  - Multiple-targeting compounds might reduce complex polypharmacy of multifactorial diseases, such as diabetes, and contribute to the greater therapeutic success. Targeting reactive oxygen species-producing enzymes, as xanthine oxidase (XO), might suppress progression of diabetes-associated vascular complications. In this study a small series of benzimidazole derivatives (1-9) was evaluated for inhibitory activity against dipeptidyl peptidase-4 (DPP-4) and XO. One 1,3-disubstituted-benzimidazole-2-imine (5) and 1,3-thiazolo [3,2-a] benzimidazolone derivative (8) were shown as effective dual DPP-4 and XO inhibitors, with IC50 values lower than 200 mu M, and predicted binding modes with both target enzymes. Both selected dual inhibitors (compounds 5 and 8) did not show cytotoxicity to a greater extent on Caco-2 cells even at concentration of 250 mu M. These structures represent new non-purine scaffolds bearing two therapeutic functionalities, being DPP-4 and XO inhibitors, more favorable in comparison to DPP-4 inhibitors with DPP-4 as a single target due to pleiotropic effects of XO inhibition.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - Benzimidazole-based dual dipeptidyl peptidase-4 and xanthine oxidase inhibitors
VL  - 315
DO  - 10.1016/j.cbi.2019.108873
ER  - 

@article{
author = "Tomović, Katarina and Ilić, Budimir S. and Smelcerović, Zaklina and Miljković, Marija and Yancheva, Denitsa and Kojić, Milan and Mavrova, Anelia Ts and Kocić, Gordana and Smelcerović, Andrija",
year = "2020",
abstract = "Multiple-targeting compounds might reduce complex polypharmacy of multifactorial diseases, such as diabetes, and contribute to the greater therapeutic success. Targeting reactive oxygen species-producing enzymes, as xanthine oxidase (XO), might suppress progression of diabetes-associated vascular complications. In this study a small series of benzimidazole derivatives (1-9) was evaluated for inhibitory activity against dipeptidyl peptidase-4 (DPP-4) and XO. One 1,3-disubstituted-benzimidazole-2-imine (5) and 1,3-thiazolo [3,2-a] benzimidazolone derivative (8) were shown as effective dual DPP-4 and XO inhibitors, with IC50 values lower than 200 mu M, and predicted binding modes with both target enzymes. Both selected dual inhibitors (compounds 5 and 8) did not show cytotoxicity to a greater extent on Caco-2 cells even at concentration of 250 mu M. These structures represent new non-purine scaffolds bearing two therapeutic functionalities, being DPP-4 and XO inhibitors, more favorable in comparison to DPP-4 inhibitors with DPP-4 as a single target due to pleiotropic effects of XO inhibition.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "Benzimidazole-based dual dipeptidyl peptidase-4 and xanthine oxidase inhibitors",
volume = "315",
doi = "10.1016/j.cbi.2019.108873"
}

Tomović, K., Ilić, B. S., Smelcerović, Z., Miljković, M., Yancheva, D., Kojić, M., Mavrova, A. T., Kocić, G.,& Smelcerović, A.. (2020). Benzimidazole-based dual dipeptidyl peptidase-4 and xanthine oxidase inhibitors. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 315.
https://doi.org/10.1016/j.cbi.2019.108873

Tomović K, Ilić BS, Smelcerović Z, Miljković M, Yancheva D, Kojić M, Mavrova AT, Kocić G, Smelcerović A. Benzimidazole-based dual dipeptidyl peptidase-4 and xanthine oxidase inhibitors. in Chemico-Biological Interactions. 2020;315.
doi:10.1016/j.cbi.2019.108873 .

Tomović, Katarina, Ilić, Budimir S., Smelcerović, Zaklina, Miljković, Marija, Yancheva, Denitsa, Kojić, Milan, Mavrova, Anelia Ts, Kocić, Gordana, Smelcerović, Andrija, "Benzimidazole-based dual dipeptidyl peptidase-4 and xanthine oxidase inhibitors" in Chemico-Biological Interactions, 315 (2020),
https://doi.org/10.1016/j.cbi.2019.108873 . .

TY  - JOUR
AU  - Vukotić, Goran
AU  - Polović, Natalija
AU  - Mirković, Nemanja
AU  - Jovčić, Branko
AU  - Stanisavljević, Nemanja
AU  - Fira, Đorđe
AU  - Kojić, Milan
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1251
AB  - In our previous study we demonstrated that proteinase PrtP is able to impair bacteriocin LcnB activity, despite being produced by the same organism and encoded by the same plasmid. However, precise mechanism of this action, i.e., the exact cleavage site within LcnB bacteriocin, as well as its effect on antimicrobial activity of the resulting peptide remained vague. Here we further explored the interplay between these two proteins and defined, using mass spectrometry, that this unusual hydrolysis indeed occurs in vivo, between the sixth and seventh amino acid on the N terminus of LcnB. To address whether the cleaved form of LcnB retains any level of activity, both recombinant and chemically synthesized variant of truncated LcnB were engineered and produced, but demonstrated no antimicrobial activity. When LcnB was recombinantly overexpressed and subjected to PrtP digestion, the change in its antimicrobial activity was monitored and the degradation products analyzed with reverse-phase high-pressure liquid chromatography. The results confirmed the inactivity of the truncated LcnB and additionally corroborated the PrtP cleavage site in LcnB bacteriocin. In addition, it was demonstrated that, once truncated, LcnB is not able to bind its receptor and is susceptible to additional hydrolysis. This is the first report on proteolytic inactivation of bacteriocins inside the same bacterial host.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. lactis BGMN1-501
VL  - 10
DO  - 10.3389/fmicb.2019.00874
ER  - 

@article{
author = "Vukotić, Goran and Polović, Natalija and Mirković, Nemanja and Jovčić, Branko and Stanisavljević, Nemanja and Fira, Đorđe and Kojić, Milan",
year = "2019",
abstract = "In our previous study we demonstrated that proteinase PrtP is able to impair bacteriocin LcnB activity, despite being produced by the same organism and encoded by the same plasmid. However, precise mechanism of this action, i.e., the exact cleavage site within LcnB bacteriocin, as well as its effect on antimicrobial activity of the resulting peptide remained vague. Here we further explored the interplay between these two proteins and defined, using mass spectrometry, that this unusual hydrolysis indeed occurs in vivo, between the sixth and seventh amino acid on the N terminus of LcnB. To address whether the cleaved form of LcnB retains any level of activity, both recombinant and chemically synthesized variant of truncated LcnB were engineered and produced, but demonstrated no antimicrobial activity. When LcnB was recombinantly overexpressed and subjected to PrtP digestion, the change in its antimicrobial activity was monitored and the degradation products analyzed with reverse-phase high-pressure liquid chromatography. The results confirmed the inactivity of the truncated LcnB and additionally corroborated the PrtP cleavage site in LcnB bacteriocin. In addition, it was demonstrated that, once truncated, LcnB is not able to bind its receptor and is susceptible to additional hydrolysis. This is the first report on proteolytic inactivation of bacteriocins inside the same bacterial host.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. lactis BGMN1-501",
volume = "10",
doi = "10.3389/fmicb.2019.00874"
}

Vukotić, G., Polović, N., Mirković, N., Jovčić, B., Stanisavljević, N., Fira, Đ.,& Kojić, M.. (2019). Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. lactis BGMN1-501. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fmicb.2019.00874

Vukotić G, Polović N, Mirković N, Jovčić B, Stanisavljević N, Fira Đ, Kojić M. Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. lactis BGMN1-501. in Frontiers in Microbiology. 2019;10.
doi:10.3389/fmicb.2019.00874 .

Vukotić, Goran, Polović, Natalija, Mirković, Nemanja, Jovčić, Branko, Stanisavljević, Nemanja, Fira, Đorđe, Kojić, Milan, "Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. lactis BGMN1-501" in Frontiers in Microbiology, 10 (2019),
https://doi.org/10.3389/fmicb.2019.00874 . .

TY  - JOUR
AU  - Malešević, Milka
AU  - Di Lorenzo, Flaviana
AU  - Filipić, Brankica
AU  - Stanisavljević, Nemanja
AU  - Novović, Katarina
AU  - Šenerović, Lidija
AU  - Polović, Natalija
AU  - Molinaro, Antonio
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1217
AB  - Pseudomonas aeruginosa is one of the most common opportunistic pathogens that use quorum sensing (QS) system to regulate virulence factors expression and biofilm development. Delftia sp. 11304 was selected among 663 Gram-negative clinical isolates based on its QS inhibitory activity against P. aeruginosa MMA83 clinical isolate. Whole genome sequencing identified this isolate as D. tsuruhatensis and revealed genetic armamentarium of virulence factors and antibiotic resistance determinants. Ethyl acetate extract of D. tsuruhatensis 11304 culture supernatant (QSI extract) prevented biofilm formation of P. aeruginosa MMA83, but was unable to cause biofilm decomposition. QSI extract showed a synergistic effect in combination with meropenem and gentamycin, against P. aeruginosa MMA83. A dose-dependent reduction of the virulence factors: elastase, rhamnolipid and pyocyanin production by P. aeruginosa MMA83 and significant downregulation of lasI, lasR, rhlI, rhlR, pqs and mvfR expression were observed. Matrix-assisted Laser Desorption Ionization (MALDI) mass spectrometry of D. tsuruhatensis 11304 QSI extract revealed the presence of N-acyl homoserine lactones (AHL) with chain lengths of C12 to C18. The main ion peak was identified as N-octadecanoylhomoserine lactone (C-18-HSL). Commercial C-18-HSL (20 mu M) reduced pyocyanin production as well as mRNA level of the lasI gene. A novel AHL species, dihydroxy-N-octadecanoylhomoserine lactone, was also described.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones
VL  - 9
DO  - 10.1038/s41598-019-52955-3
ER  - 

@article{
author = "Malešević, Milka and Di Lorenzo, Flaviana and Filipić, Brankica and Stanisavljević, Nemanja and Novović, Katarina and Šenerović, Lidija and Polović, Natalija and Molinaro, Antonio and Kojić, Milan and Jovčić, Branko",
year = "2019",
abstract = "Pseudomonas aeruginosa is one of the most common opportunistic pathogens that use quorum sensing (QS) system to regulate virulence factors expression and biofilm development. Delftia sp. 11304 was selected among 663 Gram-negative clinical isolates based on its QS inhibitory activity against P. aeruginosa MMA83 clinical isolate. Whole genome sequencing identified this isolate as D. tsuruhatensis and revealed genetic armamentarium of virulence factors and antibiotic resistance determinants. Ethyl acetate extract of D. tsuruhatensis 11304 culture supernatant (QSI extract) prevented biofilm formation of P. aeruginosa MMA83, but was unable to cause biofilm decomposition. QSI extract showed a synergistic effect in combination with meropenem and gentamycin, against P. aeruginosa MMA83. A dose-dependent reduction of the virulence factors: elastase, rhamnolipid and pyocyanin production by P. aeruginosa MMA83 and significant downregulation of lasI, lasR, rhlI, rhlR, pqs and mvfR expression were observed. Matrix-assisted Laser Desorption Ionization (MALDI) mass spectrometry of D. tsuruhatensis 11304 QSI extract revealed the presence of N-acyl homoserine lactones (AHL) with chain lengths of C12 to C18. The main ion peak was identified as N-octadecanoylhomoserine lactone (C-18-HSL). Commercial C-18-HSL (20 mu M) reduced pyocyanin production as well as mRNA level of the lasI gene. A novel AHL species, dihydroxy-N-octadecanoylhomoserine lactone, was also described.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones",
volume = "9",
doi = "10.1038/s41598-019-52955-3"
}

Malešević, M., Di Lorenzo, F., Filipić, B., Stanisavljević, N., Novović, K., Šenerović, L., Polović, N., Molinaro, A., Kojić, M.,& Jovčić, B.. (2019). Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones. in Scientific Reports
Nature Publishing Group, London., 9.
https://doi.org/10.1038/s41598-019-52955-3

Malešević M, Di Lorenzo F, Filipić B, Stanisavljević N, Novović K, Šenerović L, Polović N, Molinaro A, Kojić M, Jovčić B. Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones. in Scientific Reports. 2019;9.
doi:10.1038/s41598-019-52955-3 .

Malešević, Milka, Di Lorenzo, Flaviana, Filipić, Brankica, Stanisavljević, Nemanja, Novović, Katarina, Šenerović, Lidija, Polović, Natalija, Molinaro, Antonio, Kojić, Milan, Jovčić, Branko, "Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones" in Scientific Reports, 9 (2019),
https://doi.org/10.1038/s41598-019-52955-3 . .

TY  - JOUR
AU  - Tomović, Katarina
AU  - Ilić, Budimir S.
AU  - Miljković, Marija
AU  - Dimov, Stefan
AU  - Yancheva, Denitsa
AU  - Kojić, Milan
AU  - Mavrova, Anelia T.
AU  - Kocić, Gordana
AU  - Smelcerović, Andrija
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1396
AB  - A small library of benzo[4,5]thieno[2,3-d]pyrimidine phthalimide and amine derivatives was evaluated for inhibitory activity against dipeptidyl peptidase-4 (DPP-4). The phthalimide derivatives exhibited better activity than the amine precursors, with 2-(2-(3-chlorobenzyl)-5,6,7,8-tetrahydrobenzo[4,5]thieno[2,3-d]pyrimidin-4-yl)isoindoline-1,3-dione (compound 14) as the most effective inhibitor (IC50 = 34.17 +/- 5.11 mu M). The five most potent selected inhibitors did not show cytotoxicity to a greater extent on Caco-2 cells, even at a concentration of 250 mu M. Compound 14 is considered as a novel representative of the rare noncompetitive DPP-4 inhibitors. Molecular docking and dynamics simulation indicated the importance of the Tyr547, Lys554, and Trp629 residues of DPP-4 in the formation of the enzyme-inhibitor complex. These observations could be potentially utilized for the rational design and optimization of novel (structurally similar, with phthalimide moiety, or different) noncompetitive DPP-4 inhibitors, which are anyway rare, but favorable in terms of the saturation of substrate competition.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Archiv Der Pharmazie
T1  - Benzo[4,5]thieno[2,3-d]pyrimidine phthalimide derivative, one of the rare noncompetitive inhibitors of dipeptidyl peptidase-4
IS  - 1
VL  - 353
DO  - 10.1002/ardp.201900238
ER  - 

@article{
author = "Tomović, Katarina and Ilić, Budimir S. and Miljković, Marija and Dimov, Stefan and Yancheva, Denitsa and Kojić, Milan and Mavrova, Anelia T. and Kocić, Gordana and Smelcerović, Andrija",
year = "2019",
abstract = "A small library of benzo[4,5]thieno[2,3-d]pyrimidine phthalimide and amine derivatives was evaluated for inhibitory activity against dipeptidyl peptidase-4 (DPP-4). The phthalimide derivatives exhibited better activity than the amine precursors, with 2-(2-(3-chlorobenzyl)-5,6,7,8-tetrahydrobenzo[4,5]thieno[2,3-d]pyrimidin-4-yl)isoindoline-1,3-dione (compound 14) as the most effective inhibitor (IC50 = 34.17 +/- 5.11 mu M). The five most potent selected inhibitors did not show cytotoxicity to a greater extent on Caco-2 cells, even at a concentration of 250 mu M. Compound 14 is considered as a novel representative of the rare noncompetitive DPP-4 inhibitors. Molecular docking and dynamics simulation indicated the importance of the Tyr547, Lys554, and Trp629 residues of DPP-4 in the formation of the enzyme-inhibitor complex. These observations could be potentially utilized for the rational design and optimization of novel (structurally similar, with phthalimide moiety, or different) noncompetitive DPP-4 inhibitors, which are anyway rare, but favorable in terms of the saturation of substrate competition.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Archiv Der Pharmazie",
title = "Benzo[4,5]thieno[2,3-d]pyrimidine phthalimide derivative, one of the rare noncompetitive inhibitors of dipeptidyl peptidase-4",
number = "1",
volume = "353",
doi = "10.1002/ardp.201900238"
}

Tomović, K., Ilić, B. S., Miljković, M., Dimov, S., Yancheva, D., Kojić, M., Mavrova, A. T., Kocić, G.,& Smelcerović, A.. (2019). Benzo[4,5]thieno[2,3-d]pyrimidine phthalimide derivative, one of the rare noncompetitive inhibitors of dipeptidyl peptidase-4. in Archiv Der Pharmazie
Wiley-V C H Verlag Gmbh, Weinheim., 353(1).
https://doi.org/10.1002/ardp.201900238

Tomović K, Ilić BS, Miljković M, Dimov S, Yancheva D, Kojić M, Mavrova AT, Kocić G, Smelcerović A. Benzo[4,5]thieno[2,3-d]pyrimidine phthalimide derivative, one of the rare noncompetitive inhibitors of dipeptidyl peptidase-4. in Archiv Der Pharmazie. 2019;353(1).
doi:10.1002/ardp.201900238 .

Tomović, Katarina, Ilić, Budimir S., Miljković, Marija, Dimov, Stefan, Yancheva, Denitsa, Kojić, Milan, Mavrova, Anelia T., Kocić, Gordana, Smelcerović, Andrija, "Benzo[4,5]thieno[2,3-d]pyrimidine phthalimide derivative, one of the rare noncompetitive inhibitors of dipeptidyl peptidase-4" in Archiv Der Pharmazie, 353, no. 1 (2019),
https://doi.org/10.1002/ardp.201900238 . .

TY  - JOUR
AU  - Zavisić, Gordana N.
AU  - Petricević, Sasa M.
AU  - Ristić, Slavica M.
AU  - Rikalović, Milena G.
AU  - Jovanović-Ljesković, Nataša M.
AU  - Begović, Jelena
AU  - Strahinić, Ivana
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1268
AB  - The present study was dedicated to determining probiotic potential of a human isolate G-4, originated from meconium. The isolate was identified using morphological, physiological and biochemical assays and molecular method based on 16S rRNA gene sequencing. In order to evaluate its probiotic properties in vitro tests were performed: the survival in simulated gastrointestinal conditions, adhesion to hexadecane, and antimicrobial activity. Safety aspects of the isolate were examined by testing toxicity, gastrointestinal tolerance and bacterial translocation in vivo, as well as hemolytic activity in vitro. The isolate G-4, identified as Lactobacillus fermentum, showed viability in artificial gastric and intestinal juice (low degree of cell viability reduction for 0.69 and 1.30 logCFU mL(-1) units, respectively), moderate adhesion to hexadecane (39 +/- 2.1 %), and antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serotype Abony and Clostridium sporogenes, due to production of lactic acid (9.80 g L-1). No signs of toxicity, bacterial translocation, hemolytic activity, were observed.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns
EP  - 376
IS  - 4
SP  - 365
VL  - 84
DO  - 10.2298/JSC181105015Z
ER  - 

@article{
author = "Zavisić, Gordana N. and Petricević, Sasa M. and Ristić, Slavica M. and Rikalović, Milena G. and Jovanović-Ljesković, Nataša M. and Begović, Jelena and Strahinić, Ivana",
year = "2019",
abstract = "The present study was dedicated to determining probiotic potential of a human isolate G-4, originated from meconium. The isolate was identified using morphological, physiological and biochemical assays and molecular method based on 16S rRNA gene sequencing. In order to evaluate its probiotic properties in vitro tests were performed: the survival in simulated gastrointestinal conditions, adhesion to hexadecane, and antimicrobial activity. Safety aspects of the isolate were examined by testing toxicity, gastrointestinal tolerance and bacterial translocation in vivo, as well as hemolytic activity in vitro. The isolate G-4, identified as Lactobacillus fermentum, showed viability in artificial gastric and intestinal juice (low degree of cell viability reduction for 0.69 and 1.30 logCFU mL(-1) units, respectively), moderate adhesion to hexadecane (39 +/- 2.1 %), and antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serotype Abony and Clostridium sporogenes, due to production of lactic acid (9.80 g L-1). No signs of toxicity, bacterial translocation, hemolytic activity, were observed.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns",
pages = "376-365",
number = "4",
volume = "84",
doi = "10.2298/JSC181105015Z"
}

Zavisić, G. N., Petricević, S. M., Ristić, S. M., Rikalović, M. G., Jovanović-Ljesković, N. M., Begović, J.,& Strahinić, I.. (2019). Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(4), 365-376.
https://doi.org/10.2298/JSC181105015Z

Zavisić GN, Petricević SM, Ristić SM, Rikalović MG, Jovanović-Ljesković NM, Begović J, Strahinić I. Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns. in Journal of the Serbian Chemical Society. 2019;84(4):365-376.
doi:10.2298/JSC181105015Z .

Zavisić, Gordana N., Petricević, Sasa M., Ristić, Slavica M., Rikalović, Milena G., Jovanović-Ljesković, Nataša M., Begović, Jelena, Strahinić, Ivana, "Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns" in Journal of the Serbian Chemical Society, 84, no. 4 (2019):365-376,
https://doi.org/10.2298/JSC181105015Z . .

TY  - JOUR
AU  - Miljković, Marija
AU  - Jovanović, Sofija
AU  - O'Connor, Paula M.
AU  - Mirković, Nemanja
AU  - Jovčić, Branko
AU  - Filipić, Brankica
AU  - Dinić, Miroslav
AU  - Studholme, David John
AU  - Fira, Đorđe
AU  - Cotter, Paul D.
AU  - Kojić, Milan
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1264
AB  - Bacteria active against multi-drug resistant pathogens, isolated by direct selection of colonies from clover silage samples, produce zones of inhibition against two Gram-negative (Klebsiella pneumoniae Ni9 and Pseudomonas aeruginosa MMA83) and two Gram-positive (Staphylococcus aureus ATCC25923 and Listeria monocytogenes ATCC19111) pathogens. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 produced the largest zones of inhibition against all four pathogens when grown in LB broth with aeration at 37 degrees C. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 were identified as Brevibacillus laterosporus and pulsed field gel electrophoresis and extracellular protein profiles showed that three different strains (BGSP7, BGSP9 and BGSP11) were isolated. A semi-native SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) gel overlay assay showed that BGSP7 and BGSP9 produce small antimicrobial molecules of about 1.5 kDa, while BGSP11 produces antimicrobial molecules of 1.5 and 6 kDa active against S. aureus ATCC25923. Amino acid analysis of two antimicrobial molecules (1583.73 Da; from BGSP7 and 1556.31 Da; from BGSP11) revealed that they have a similar composition and differ only by virtue of the presence of a methionine which is present only in BGSP11 molecule. Genome sequencing of the three isolates revealed the presence of gene clusters associated with the production of non-ribosomally synthesized peptides (brevibacillin, bogorol, gramicidin S, plipastatin and tyrocin) and bacteriocins (laterosporulin, a lactococcin 972-like bacteriocin, as well as putative linocin M18, sactipeptide, UviB and lantipeptide-like molecules). Ultimately, the purification of a number of antimicrobial molecules from each isolate suggests that they can be considered as potent biocontrol strains that produce an arsenal of antimicrobial molecules active against Gram-positive and Gram-negative multi-resistant pathogens, fungi and insects.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials
IS  - 5
VL  - 14
DO  - 10.1371/journal.pone.0216773
ER  - 

@article{
author = "Miljković, Marija and Jovanović, Sofija and O'Connor, Paula M. and Mirković, Nemanja and Jovčić, Branko and Filipić, Brankica and Dinić, Miroslav and Studholme, David John and Fira, Đorđe and Cotter, Paul D. and Kojić, Milan",
year = "2019",
abstract = "Bacteria active against multi-drug resistant pathogens, isolated by direct selection of colonies from clover silage samples, produce zones of inhibition against two Gram-negative (Klebsiella pneumoniae Ni9 and Pseudomonas aeruginosa MMA83) and two Gram-positive (Staphylococcus aureus ATCC25923 and Listeria monocytogenes ATCC19111) pathogens. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 produced the largest zones of inhibition against all four pathogens when grown in LB broth with aeration at 37 degrees C. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 were identified as Brevibacillus laterosporus and pulsed field gel electrophoresis and extracellular protein profiles showed that three different strains (BGSP7, BGSP9 and BGSP11) were isolated. A semi-native SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) gel overlay assay showed that BGSP7 and BGSP9 produce small antimicrobial molecules of about 1.5 kDa, while BGSP11 produces antimicrobial molecules of 1.5 and 6 kDa active against S. aureus ATCC25923. Amino acid analysis of two antimicrobial molecules (1583.73 Da; from BGSP7 and 1556.31 Da; from BGSP11) revealed that they have a similar composition and differ only by virtue of the presence of a methionine which is present only in BGSP11 molecule. Genome sequencing of the three isolates revealed the presence of gene clusters associated with the production of non-ribosomally synthesized peptides (brevibacillin, bogorol, gramicidin S, plipastatin and tyrocin) and bacteriocins (laterosporulin, a lactococcin 972-like bacteriocin, as well as putative linocin M18, sactipeptide, UviB and lantipeptide-like molecules). Ultimately, the purification of a number of antimicrobial molecules from each isolate suggests that they can be considered as potent biocontrol strains that produce an arsenal of antimicrobial molecules active against Gram-positive and Gram-negative multi-resistant pathogens, fungi and insects.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials",
number = "5",
volume = "14",
doi = "10.1371/journal.pone.0216773"
}

Miljković, M., Jovanović, S., O'Connor, P. M., Mirković, N., Jovčić, B., Filipić, B., Dinić, M., Studholme, D. J., Fira, Đ., Cotter, P. D.,& Kojić, M.. (2019). Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials. in PLoS One
Public Library Science, San Francisco., 14(5).
https://doi.org/10.1371/journal.pone.0216773

Miljković M, Jovanović S, O'Connor PM, Mirković N, Jovčić B, Filipić B, Dinić M, Studholme DJ, Fira Đ, Cotter PD, Kojić M. Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials. in PLoS One. 2019;14(5).
doi:10.1371/journal.pone.0216773 .

Miljković, Marija, Jovanović, Sofija, O'Connor, Paula M., Mirković, Nemanja, Jovčić, Branko, Filipić, Brankica, Dinić, Miroslav, Studholme, David John, Fira, Đorđe, Cotter, Paul D., Kojić, Milan, "Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials" in PLoS One, 14, no. 5 (2019),
https://doi.org/10.1371/journal.pone.0216773 . .

TY  - JOUR
AU  - Pesić, Mirjana B.
AU  - Milincić, Danijel D.
AU  - Kostić, Aleksandar Z.
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Kojić, Milan
AU  - Gašić, Uroš
AU  - Barac, Miroljub B.
AU  - Stanojević, Sladana P.
AU  - Popović, Dusanka A.
AU  - Banjac, Nebojša R.
AU  - Tesić, Zivoslav Lj.
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1609
AB  - The aim of this study was to evaluate the effect of enriching a complex food matrix (FM) with grape extracts on polyphenol content, composition, bioaccessibility and antioxidant activity during digestion. The grape extracts and FM were separately tested under the same conditions as controls. The FM by itself contains a significant amount of phenolic acids and flavonols, influencing the final recovery of polyphenols from grape extracts. The FM significantly increased the total recovery of polyphenols after digestion of grape seed extracts compared to those digested without the FM; however, a low recovery of proantocyanidins and total flavonoids was observed. Digestive fluids and FM compounds significantly increased the total polyphenol content of grape digests and significantly contributed to their ABTS(center dot+) scavenging activity and ferrous-ion-chelating capacity. The present study suggested that enrichment of meat-and cereal-based products with grape polyphenol extracts could be a good strategy to formulate a healthier diet.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?
EP  - 44
SP  - 28
VL  - 284
DO  - 10.1016/j.foodchem.2019.01.107
ER  - 

@article{
author = "Pesić, Mirjana B. and Milincić, Danijel D. and Kostić, Aleksandar Z. and Stanisavljević, Nemanja and Vukotić, Goran and Kojić, Milan and Gašić, Uroš and Barac, Miroljub B. and Stanojević, Sladana P. and Popović, Dusanka A. and Banjac, Nebojša R. and Tesić, Zivoslav Lj.",
year = "2019",
abstract = "The aim of this study was to evaluate the effect of enriching a complex food matrix (FM) with grape extracts on polyphenol content, composition, bioaccessibility and antioxidant activity during digestion. The grape extracts and FM were separately tested under the same conditions as controls. The FM by itself contains a significant amount of phenolic acids and flavonols, influencing the final recovery of polyphenols from grape extracts. The FM significantly increased the total recovery of polyphenols after digestion of grape seed extracts compared to those digested without the FM; however, a low recovery of proantocyanidins and total flavonoids was observed. Digestive fluids and FM compounds significantly increased the total polyphenol content of grape digests and significantly contributed to their ABTS(center dot+) scavenging activity and ferrous-ion-chelating capacity. The present study suggested that enrichment of meat-and cereal-based products with grape polyphenol extracts could be a good strategy to formulate a healthier diet.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?",
pages = "44-28",
volume = "284",
doi = "10.1016/j.foodchem.2019.01.107"
}

Pesić, M. B., Milincić, D. D., Kostić, A. Z., Stanisavljević, N., Vukotić, G., Kojić, M., Gašić, U., Barac, M. B., Stanojević, S. P., Popović, D. A., Banjac, N. R.,& Tesić, Z. Lj.. (2019). In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?. in Food Chemistry
Elsevier Sci Ltd, Oxford., 284, 28-44.
https://doi.org/10.1016/j.foodchem.2019.01.107

Pesić MB, Milincić DD, Kostić AZ, Stanisavljević N, Vukotić G, Kojić M, Gašić U, Barac MB, Stanojević SP, Popović DA, Banjac NR, Tesić ZL. In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?. in Food Chemistry. 2019;284:28-44.
doi:10.1016/j.foodchem.2019.01.107 .

Pesić, Mirjana B., Milincić, Danijel D., Kostić, Aleksandar Z., Stanisavljević, Nemanja, Vukotić, Goran, Kojić, Milan, Gašić, Uroš, Barac, Miroljub B., Stanojević, Sladana P., Popović, Dusanka A., Banjac, Nebojša R., Tesić, Zivoslav Lj., "In vitro digestion of meat- and cereal-based food matrix enriched with grape extracts: How are polyphenol composition, bioaccessibility and antioxidant activity affected?" in Food Chemistry, 284 (2019):28-44,
https://doi.org/10.1016/j.foodchem.2019.01.107 . .

TY  - JOUR
AU  - Soković Bajić, Svetlana
AU  - Đokić, Jelena
AU  - Dinić, Miroslav
AU  - Veljović, Katarina
AU  - Golić, Nataša
AU  - Mihajlović, Sanja
AU  - Tolinački, Maja
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1298
AB  - Probiotic bacteria are recognized for their health-promoting properties, including maintenance of gut epithelial integrity and host immune system homeostasis. Taking into account the beneficial health-promoting effects of GABA, the presence of the gadB gene, encoding glutamate decarboxylase that converts L-glutamate to GABA, was analyzed in Lactic Acid Bacteria (LAB) natural isolates from Zlatar cheese. The results revealed that 52% of tested Lactobacillus spp. and 8% of Lactococcus spp. isolates harbor the gadB gene. Qualitative and quantitative analysis of GABA production performed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) revealed the highest GABA production by Lactobacillus brevis BGZLS10-17. Since high GABA-producing LAB natural isolates are the most valuable source of naturally produced GABA, the probiotic properties of BGZLS10-17 were characterized. This study demonstrated high adhesion of BGZLS10-17 strain to Caco2 cells and the ability to decrease the adhesion of Escherichia coli ATCC25922 and Salmonella enterica C29039. Treatment of differentiated Caco-2 cells monolayer with BGZLS10-17 supernatant containing GABA alleviated inflammation (production of IL-8) caused by IL-1 beta and significantly stimulated the expression of tight junction proteins (zonulin, occludin, and claudin 4), as well as the expression of TGF-b cytokine leading to the conclusion that immunosuppression and strengthening the tight junctions can have significant role in the maintenance of intestinal epithelial barrier integrity. Taken together the results obtained in this study support the idea that using of GABA producing BGZLS10-17 probiotic strain could be a good strategy to modulate immunological response in various inflammatory diseases, and at the same time, it could be a good candidate for adjunct starter culture for production of GABA-enriched dairy foods and beverages offering new perspectives in designing the novel functional foods.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - GABA-Producing Natural Dairy Isolate From Artisanal Zlatar Cheese Attenuates Gut Inflammation and Strengthens Gut Epithelial Barrier in vitro
VL  - 10
DO  - 10.3389/fmicb.2019.00527
ER  - 

@article{
author = "Soković Bajić, Svetlana and Đokić, Jelena and Dinić, Miroslav and Veljović, Katarina and Golić, Nataša and Mihajlović, Sanja and Tolinački, Maja",
year = "2019",
abstract = "Probiotic bacteria are recognized for their health-promoting properties, including maintenance of gut epithelial integrity and host immune system homeostasis. Taking into account the beneficial health-promoting effects of GABA, the presence of the gadB gene, encoding glutamate decarboxylase that converts L-glutamate to GABA, was analyzed in Lactic Acid Bacteria (LAB) natural isolates from Zlatar cheese. The results revealed that 52% of tested Lactobacillus spp. and 8% of Lactococcus spp. isolates harbor the gadB gene. Qualitative and quantitative analysis of GABA production performed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) revealed the highest GABA production by Lactobacillus brevis BGZLS10-17. Since high GABA-producing LAB natural isolates are the most valuable source of naturally produced GABA, the probiotic properties of BGZLS10-17 were characterized. This study demonstrated high adhesion of BGZLS10-17 strain to Caco2 cells and the ability to decrease the adhesion of Escherichia coli ATCC25922 and Salmonella enterica C29039. Treatment of differentiated Caco-2 cells monolayer with BGZLS10-17 supernatant containing GABA alleviated inflammation (production of IL-8) caused by IL-1 beta and significantly stimulated the expression of tight junction proteins (zonulin, occludin, and claudin 4), as well as the expression of TGF-b cytokine leading to the conclusion that immunosuppression and strengthening the tight junctions can have significant role in the maintenance of intestinal epithelial barrier integrity. Taken together the results obtained in this study support the idea that using of GABA producing BGZLS10-17 probiotic strain could be a good strategy to modulate immunological response in various inflammatory diseases, and at the same time, it could be a good candidate for adjunct starter culture for production of GABA-enriched dairy foods and beverages offering new perspectives in designing the novel functional foods.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "GABA-Producing Natural Dairy Isolate From Artisanal Zlatar Cheese Attenuates Gut Inflammation and Strengthens Gut Epithelial Barrier in vitro",
volume = "10",
doi = "10.3389/fmicb.2019.00527"
}

Soković Bajić, S., Đokić, J., Dinić, M., Veljović, K., Golić, N., Mihajlović, S.,& Tolinački, M.. (2019). GABA-Producing Natural Dairy Isolate From Artisanal Zlatar Cheese Attenuates Gut Inflammation and Strengthens Gut Epithelial Barrier in vitro. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fmicb.2019.00527

Soković Bajić S, Đokić J, Dinić M, Veljović K, Golić N, Mihajlović S, Tolinački M. GABA-Producing Natural Dairy Isolate From Artisanal Zlatar Cheese Attenuates Gut Inflammation and Strengthens Gut Epithelial Barrier in vitro. in Frontiers in Microbiology. 2019;10.
doi:10.3389/fmicb.2019.00527 .

Soković Bajić, Svetlana, Đokić, Jelena, Dinić, Miroslav, Veljović, Katarina, Golić, Nataša, Mihajlović, Sanja, Tolinački, Maja, "GABA-Producing Natural Dairy Isolate From Artisanal Zlatar Cheese Attenuates Gut Inflammation and Strengthens Gut Epithelial Barrier in vitro" in Frontiers in Microbiology, 10 (2019),
https://doi.org/10.3389/fmicb.2019.00527 . .