TY  - JOUR
AU  - Buzurović, Uros
AU  - Tomović, Gordana
AU  - Niketić, Marjan
AU  - Bogdanović, Sandro
AU  - Aleksić, Jelena M.
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1389
AB  - Goniolimon species are mainly components of the Eurasian steppe or steppe-like rocky vegetation, with some taxa occurring also in south-eastern Europe and northern Africa. We analysed the variability of: (i) two maternally inherited plastid loci (rpl32-trnL and 3 ' rps16-5 ' trnK) in 110 individuals of six currently accepted species from the Balkans and one species from the Apennines, to provide new insights into their origin and evolutionary history; and (ii) quantitative morphological characters (14 independent characters and one ratio character) in 641 individuals of three species of which two are morphologically and ecologically similar (G. italicum and G. tataricum) and the third, G. dalmaticum, was frequently misidentified as G. tataricum in the past, to provide new taxonomic treatment for proposed G. tataricum subspecies. We delineated several quantitative and five qualitative characters studied in a more limited sample as diagnostic for the identification of four subspecies (three newly described and one in a new rank) of G. tataricum. The history of westward peripheral populations of this species in the Balkans and the Apennines was rather complex and driven by local geo-historic events. These events facilitated multiple waves of east-west expansion of lineages originating from sources outside of the Balkan Peninsula which periodically diversified and occupied localised areas in the Balkans during the Pleistocene. An initial spread of an ancient G. tataricum lineage throughout south-eastern Europe probably occurred during the Messinian Salinity Crisis. Inter- and intraspecific hybridisation/introgression, as well as retention of ancestral polymorphisms, was common in G. tataricum and related taxa over time.
PB  - Springer Wien, Wien
T2  - Plant Systematics and Evolution
T1  - Phylogeographic and taxonomic considerations on Goniolimon tataricum (Plumbaginaceae) and its relatives from south-eastern Europe and the Apennine Peninsula
EP  - 22
IS  - 2
SP  - 1
VL  - 306
DO  - 10.1007/s00606-020-01636-0
ER  - 

@article{
author = "Buzurović, Uros and Tomović, Gordana and Niketić, Marjan and Bogdanović, Sandro and Aleksić, Jelena M.",
year = "2020",
abstract = "Goniolimon species are mainly components of the Eurasian steppe or steppe-like rocky vegetation, with some taxa occurring also in south-eastern Europe and northern Africa. We analysed the variability of: (i) two maternally inherited plastid loci (rpl32-trnL and 3 ' rps16-5 ' trnK) in 110 individuals of six currently accepted species from the Balkans and one species from the Apennines, to provide new insights into their origin and evolutionary history; and (ii) quantitative morphological characters (14 independent characters and one ratio character) in 641 individuals of three species of which two are morphologically and ecologically similar (G. italicum and G. tataricum) and the third, G. dalmaticum, was frequently misidentified as G. tataricum in the past, to provide new taxonomic treatment for proposed G. tataricum subspecies. We delineated several quantitative and five qualitative characters studied in a more limited sample as diagnostic for the identification of four subspecies (three newly described and one in a new rank) of G. tataricum. The history of westward peripheral populations of this species in the Balkans and the Apennines was rather complex and driven by local geo-historic events. These events facilitated multiple waves of east-west expansion of lineages originating from sources outside of the Balkan Peninsula which periodically diversified and occupied localised areas in the Balkans during the Pleistocene. An initial spread of an ancient G. tataricum lineage throughout south-eastern Europe probably occurred during the Messinian Salinity Crisis. Inter- and intraspecific hybridisation/introgression, as well as retention of ancestral polymorphisms, was common in G. tataricum and related taxa over time.",
publisher = "Springer Wien, Wien",
journal = "Plant Systematics and Evolution",
title = "Phylogeographic and taxonomic considerations on Goniolimon tataricum (Plumbaginaceae) and its relatives from south-eastern Europe and the Apennine Peninsula",
pages = "22-1",
number = "2",
volume = "306",
doi = "10.1007/s00606-020-01636-0"
}

Buzurović, U., Tomović, G., Niketić, M., Bogdanović, S.,& Aleksić, J. M.. (2020). Phylogeographic and taxonomic considerations on Goniolimon tataricum (Plumbaginaceae) and its relatives from south-eastern Europe and the Apennine Peninsula. in Plant Systematics and Evolution
Springer Wien, Wien., 306(2), 1-22.
https://doi.org/10.1007/s00606-020-01636-0

Buzurović U, Tomović G, Niketić M, Bogdanović S, Aleksić JM. Phylogeographic and taxonomic considerations on Goniolimon tataricum (Plumbaginaceae) and its relatives from south-eastern Europe and the Apennine Peninsula. in Plant Systematics and Evolution. 2020;306(2):1-22.
doi:10.1007/s00606-020-01636-0 .

Buzurović, Uros, Tomović, Gordana, Niketić, Marjan, Bogdanović, Sandro, Aleksić, Jelena M., "Phylogeographic and taxonomic considerations on Goniolimon tataricum (Plumbaginaceae) and its relatives from south-eastern Europe and the Apennine Peninsula" in Plant Systematics and Evolution, 306, no. 2 (2020):1-22,
https://doi.org/10.1007/s00606-020-01636-0 . .

TY  - JOUR
AU  - Kerkez-Janković, Ivona
AU  - Nonić, Marina
AU  - Devetaković, Jovana
AU  - Ivetić, Vladan
AU  - Sijacić-Nikolić, Mirjana
AU  - Aleksić, Jelena M.
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1239
AB  - Selectively neutral nuclear microsatellites (SSRs) are available for Fagus sp., and have been used in F. sylvatica, an ecologically and economically most important European Fagus species. We provide a comprehensive technical overview of available Fagus sp. SSRs (185 loci), carry out meta-analysis of loci used in F. sylvatica (62 loci employed in 62 surveys), and validate a set of 16 loci in 45 individuals of this species from the central Balkans (Serbia). Erroneous usage of marker's names/authors is rather frequent, and loci successfully used in a number of studies are characterized by other authors by high prevalence of null alleles and even multilocus amplification products. Frequent occurrence of null alleles at FS4-46, used in 26 surveys to date, most likely indicates a failure to record multiple alleles at this locus. Twelve loci are reliable/informative in F. sylvatica from the Balkans (5-18 alleles/locus, H-E ranging from 0.523 to 0.850), while four loci are characterized by high prevalence of null alleles (sfc0161 and sfc1063) and multilocus amplification products (FS4-46 and Fagsyl_007038). Our findings are important for future population genetics and studies on adaptation of F. sylvatica to its environment, because the latter rely on both selectively neutral and adaptive markers under selection.
PB  - Taylor & Francis As, Oslo
T2  - Scandinavian Journal of Forest Research
T1  - Technical overview of nuclear microsatellites for Fagus sp., and their utility in F. sylvatica from the central Balkans (Serbia)
EP  - 556
IS  - 7
SP  - 545
VL  - 34
DO  - 10.1080/02827581.2019.1623305
ER  - 

@article{
author = "Kerkez-Janković, Ivona and Nonić, Marina and Devetaković, Jovana and Ivetić, Vladan and Sijacić-Nikolić, Mirjana and Aleksić, Jelena M.",
year = "2019",
abstract = "Selectively neutral nuclear microsatellites (SSRs) are available for Fagus sp., and have been used in F. sylvatica, an ecologically and economically most important European Fagus species. We provide a comprehensive technical overview of available Fagus sp. SSRs (185 loci), carry out meta-analysis of loci used in F. sylvatica (62 loci employed in 62 surveys), and validate a set of 16 loci in 45 individuals of this species from the central Balkans (Serbia). Erroneous usage of marker's names/authors is rather frequent, and loci successfully used in a number of studies are characterized by other authors by high prevalence of null alleles and even multilocus amplification products. Frequent occurrence of null alleles at FS4-46, used in 26 surveys to date, most likely indicates a failure to record multiple alleles at this locus. Twelve loci are reliable/informative in F. sylvatica from the Balkans (5-18 alleles/locus, H-E ranging from 0.523 to 0.850), while four loci are characterized by high prevalence of null alleles (sfc0161 and sfc1063) and multilocus amplification products (FS4-46 and Fagsyl_007038). Our findings are important for future population genetics and studies on adaptation of F. sylvatica to its environment, because the latter rely on both selectively neutral and adaptive markers under selection.",
publisher = "Taylor & Francis As, Oslo",
journal = "Scandinavian Journal of Forest Research",
title = "Technical overview of nuclear microsatellites for Fagus sp., and their utility in F. sylvatica from the central Balkans (Serbia)",
pages = "556-545",
number = "7",
volume = "34",
doi = "10.1080/02827581.2019.1623305"
}

Kerkez-Janković, I., Nonić, M., Devetaković, J., Ivetić, V., Sijacić-Nikolić, M.,& Aleksić, J. M.. (2019). Technical overview of nuclear microsatellites for Fagus sp., and their utility in F. sylvatica from the central Balkans (Serbia). in Scandinavian Journal of Forest Research
Taylor & Francis As, Oslo., 34(7), 545-556.
https://doi.org/10.1080/02827581.2019.1623305

Kerkez-Janković I, Nonić M, Devetaković J, Ivetić V, Sijacić-Nikolić M, Aleksić JM. Technical overview of nuclear microsatellites for Fagus sp., and their utility in F. sylvatica from the central Balkans (Serbia). in Scandinavian Journal of Forest Research. 2019;34(7):545-556.
doi:10.1080/02827581.2019.1623305 .

Kerkez-Janković, Ivona, Nonić, Marina, Devetaković, Jovana, Ivetić, Vladan, Sijacić-Nikolić, Mirjana, Aleksić, Jelena M., "Technical overview of nuclear microsatellites for Fagus sp., and their utility in F. sylvatica from the central Balkans (Serbia)" in Scandinavian Journal of Forest Research, 34, no. 7 (2019):545-556,
https://doi.org/10.1080/02827581.2019.1623305 . .

TY  - JOUR
AU  - Aleksić, Jelena M.
AU  - Skondrić, Sinisa
AU  - Lakusić, Dmitar
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1176
AB  - Capitulate inflorescence is a specific, strongly adaptive and rare feature in the genus Campanula. We studied morphologically eight capitulate Campanula taxa from the Balkans (1537 individuals/52 populations) and one more species from Caucasus at the molecular level (using chloroplast markers trnG(UCC)-trnS(GCU) and psbA-trnH, 130 individuals/58 populations) to assess their relations and evolutionary histories. Although all studied taxa were well circumscribed at both the morphological and molecular levels (except morphologically distinct but genetically invariable C. moesiaca which acquired its single haplotype via past cytoplasmic introgression from C. cervicaria), their relations inferred from the two datasets were incongruent possibly due to the homoplasy of morphological characters frequently reported in Campanula. Interspecific hybridization and introgression affected majority of studied species and may be more common in Campanula than previously thought. These processes, along with incomplete lineage sorting and retention of ancestral polymorphisms, hampered our phylogenetic reconstructions and prevented us to fully resolve species relations, and to support monophyletic origin of capitulate Campanula species. Nonetheless, several cryptic taxa were delineated, and C. daucoides was described as a new capitulate Campanula species. Different evolutionary histories and multiple glacial refugia were inferred for all species represented by multiple samples (except C. moesiaca). According to our dating, their speciation was in most cases triggered by various geo-historic events such as the uplift of the Alpide belt, Messinian Salinity Crisis, or desiccation of the Pannonian Sea/Pliocene Lakes from the central Balkans, while their further diversification was mainly driven by the onset of the Quaternary and cycles of glacials/interglacials.
PB  - Springer Wien, Wien
T2  - Plant Systematics and Evolution
T1  - Comparative phylogeography of capitulate Campanula species from the Balkans, with description of a new species, C-daucoides
EP  - 575
IS  - 4
SP  - 549
VL  - 304
DO  - 10.1007/s00606-018-1490-7
ER  - 

@article{
author = "Aleksić, Jelena M. and Skondrić, Sinisa and Lakusić, Dmitar",
year = "2018",
abstract = "Capitulate inflorescence is a specific, strongly adaptive and rare feature in the genus Campanula. We studied morphologically eight capitulate Campanula taxa from the Balkans (1537 individuals/52 populations) and one more species from Caucasus at the molecular level (using chloroplast markers trnG(UCC)-trnS(GCU) and psbA-trnH, 130 individuals/58 populations) to assess their relations and evolutionary histories. Although all studied taxa were well circumscribed at both the morphological and molecular levels (except morphologically distinct but genetically invariable C. moesiaca which acquired its single haplotype via past cytoplasmic introgression from C. cervicaria), their relations inferred from the two datasets were incongruent possibly due to the homoplasy of morphological characters frequently reported in Campanula. Interspecific hybridization and introgression affected majority of studied species and may be more common in Campanula than previously thought. These processes, along with incomplete lineage sorting and retention of ancestral polymorphisms, hampered our phylogenetic reconstructions and prevented us to fully resolve species relations, and to support monophyletic origin of capitulate Campanula species. Nonetheless, several cryptic taxa were delineated, and C. daucoides was described as a new capitulate Campanula species. Different evolutionary histories and multiple glacial refugia were inferred for all species represented by multiple samples (except C. moesiaca). According to our dating, their speciation was in most cases triggered by various geo-historic events such as the uplift of the Alpide belt, Messinian Salinity Crisis, or desiccation of the Pannonian Sea/Pliocene Lakes from the central Balkans, while their further diversification was mainly driven by the onset of the Quaternary and cycles of glacials/interglacials.",
publisher = "Springer Wien, Wien",
journal = "Plant Systematics and Evolution",
title = "Comparative phylogeography of capitulate Campanula species from the Balkans, with description of a new species, C-daucoides",
pages = "575-549",
number = "4",
volume = "304",
doi = "10.1007/s00606-018-1490-7"
}

Aleksić, J. M., Skondrić, S.,& Lakusić, D.. (2018). Comparative phylogeography of capitulate Campanula species from the Balkans, with description of a new species, C-daucoides. in Plant Systematics and Evolution
Springer Wien, Wien., 304(4), 549-575.
https://doi.org/10.1007/s00606-018-1490-7

Aleksić JM, Skondrić S, Lakusić D. Comparative phylogeography of capitulate Campanula species from the Balkans, with description of a new species, C-daucoides. in Plant Systematics and Evolution. 2018;304(4):549-575.
doi:10.1007/s00606-018-1490-7 .

Aleksić, Jelena M., Skondrić, Sinisa, Lakusić, Dmitar, "Comparative phylogeography of capitulate Campanula species from the Balkans, with description of a new species, C-daucoides" in Plant Systematics and Evolution, 304, no. 4 (2018):549-575,
https://doi.org/10.1007/s00606-018-1490-7 . .

TY  - JOUR
AU  - Bjedov, Ivana
AU  - Obratov-Petković, Dragica
AU  - Misić, Danijela
AU  - Siler, Branislav
AU  - Aleksić, Jelena M.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/865
AB  - Vaccinium myrtillus L., Vaccinium uliginosum L. and Vaccinium vitis-idaea L. are perennial, cold-adapted clonal shrubs distributed throughout Europe, northern Asia and North America. Due to their usage in food (berries) and pharmaceutical industry (berries and leaves), their natural populations are exposed to anthropogenic and other impacts that affect their genetic make-up. We analyzed 14 fragmentary distributed and small-sized peripheral populations of these species from the Balkans, which represents the southeastern-European marginal area of their wide European distributions, using RAPD molecular markers. The contemporary genetic patterns in all three species within the Balkans were generally similar, and in comparison to previous reports on populations of these species found in northward Europe, where they have a more continuous distribution, the levels of genetic diversity were more or less halved, genetic differentiation was several times higher, gene flow exceptionally low, and the expected prevalence of clonal individuals was lacking. The population dynamics of all three species within the Balkans was complex and distinct, and was characterized by a past admixture of individuals from discrete populations of the same species and interspecific hybridisation not only between V. myrtillus and V. vitis-idaea but also between V. uliginosum and V. vitis-idaea, the latter not being reported to date. Conservation measures suitable for preservation of presumably genetically distinct portions of the Balkans' gene pools of studied species have been suggested, while the utility of interspecific hybrids in breeding programs and/or in food/pharmaceutical industry is yet to be assessed.
PB  - Finnish Society of Forest Science
T2  - Silva Fennica
T1  - Genetic patterns in range-edge populations of Vaccinium species from the central Balkans: implications on conservation prospects and sustainable usage
IS  - 4
VL  - 49
DO  - 10.14214/sf.1283
ER  - 

@article{
author = "Bjedov, Ivana and Obratov-Petković, Dragica and Misić, Danijela and Siler, Branislav and Aleksić, Jelena M.",
year = "2015",
abstract = "Vaccinium myrtillus L., Vaccinium uliginosum L. and Vaccinium vitis-idaea L. are perennial, cold-adapted clonal shrubs distributed throughout Europe, northern Asia and North America. Due to their usage in food (berries) and pharmaceutical industry (berries and leaves), their natural populations are exposed to anthropogenic and other impacts that affect their genetic make-up. We analyzed 14 fragmentary distributed and small-sized peripheral populations of these species from the Balkans, which represents the southeastern-European marginal area of their wide European distributions, using RAPD molecular markers. The contemporary genetic patterns in all three species within the Balkans were generally similar, and in comparison to previous reports on populations of these species found in northward Europe, where they have a more continuous distribution, the levels of genetic diversity were more or less halved, genetic differentiation was several times higher, gene flow exceptionally low, and the expected prevalence of clonal individuals was lacking. The population dynamics of all three species within the Balkans was complex and distinct, and was characterized by a past admixture of individuals from discrete populations of the same species and interspecific hybridisation not only between V. myrtillus and V. vitis-idaea but also between V. uliginosum and V. vitis-idaea, the latter not being reported to date. Conservation measures suitable for preservation of presumably genetically distinct portions of the Balkans' gene pools of studied species have been suggested, while the utility of interspecific hybrids in breeding programs and/or in food/pharmaceutical industry is yet to be assessed.",
publisher = "Finnish Society of Forest Science",
journal = "Silva Fennica",
title = "Genetic patterns in range-edge populations of Vaccinium species from the central Balkans: implications on conservation prospects and sustainable usage",
number = "4",
volume = "49",
doi = "10.14214/sf.1283"
}

Bjedov, I., Obratov-Petković, D., Misić, D., Siler, B.,& Aleksić, J. M.. (2015). Genetic patterns in range-edge populations of Vaccinium species from the central Balkans: implications on conservation prospects and sustainable usage. in Silva Fennica
Finnish Society of Forest Science., 49(4).
https://doi.org/10.14214/sf.1283

Bjedov I, Obratov-Petković D, Misić D, Siler B, Aleksić JM. Genetic patterns in range-edge populations of Vaccinium species from the central Balkans: implications on conservation prospects and sustainable usage. in Silva Fennica. 2015;49(4).
doi:10.14214/sf.1283 .

Bjedov, Ivana, Obratov-Petković, Dragica, Misić, Danijela, Siler, Branislav, Aleksić, Jelena M., "Genetic patterns in range-edge populations of Vaccinium species from the central Balkans: implications on conservation prospects and sustainable usage" in Silva Fennica, 49, no. 4 (2015),
https://doi.org/10.14214/sf.1283 . .

TY  - JOUR
AU  - Sarac, Zorica
AU  - Dodos, Tanja
AU  - Rajcević, Nemanja
AU  - Bojović, Srdjan
AU  - Marin, Petar D.
AU  - Aleksić, Jelena M.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/835
AB  - Pinus nigra J.F. Arnold, European black pine, is a typical component of Mediterranean and sub-Mediterranean coniferous forests with highly fragmentary distribution. Western Mediterranean populations of this species have been studied genetically to date, while eastern populations from the central Balkans, which are larger and more abundant, are still genetically understudied. We analyzed seven populations of P. nigra representing all infraspecific taxa recognized within the central Balkans (subspecies nigra with varieties nigra and gocensis Dordevic; and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and banatica (Endl.) Georgescu et Ionescu), with three chloroplast microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus. Although our molecular data failed to support circumscription of studied infraspecific taxa, we found that genetic patterns at both genomes are in accordance with those found previously in westward populations of this species, that is - exceptionally high levels of genetic diversity (H-T = 0.949) and low genetic differentiation (G(ST) = 0.024) at the cpDNA level, and moderate levels of genetic diversity (H-T = 0.357) and genetic differentiation (G(ST) = 0.358) at the mtDNA level. Based on genealogical relations of mtDNA types currently present in Balkans' and Iberian/African populations, we inferred that the ancestral gene pool of P. nigra already harbored polymorphism at position 328 prior to the divergence to two lineages currently present in westward and eastward parts of the species range distribution. Subsequent occurrence of three mutations, which distinguish these two lineages, suggests their long-term isolation.
PB  - Finnish Soc Forest Science-Natural Resources Inst Finland, Vantaa
T2  - Silva Fennica
T1  - Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data
IS  - 5
VL  - 49
DO  - 10.14214/sf.1415
ER  - 

@article{
author = "Sarac, Zorica and Dodos, Tanja and Rajcević, Nemanja and Bojović, Srdjan and Marin, Petar D. and Aleksić, Jelena M.",
year = "2015",
abstract = "Pinus nigra J.F. Arnold, European black pine, is a typical component of Mediterranean and sub-Mediterranean coniferous forests with highly fragmentary distribution. Western Mediterranean populations of this species have been studied genetically to date, while eastern populations from the central Balkans, which are larger and more abundant, are still genetically understudied. We analyzed seven populations of P. nigra representing all infraspecific taxa recognized within the central Balkans (subspecies nigra with varieties nigra and gocensis Dordevic; and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and banatica (Endl.) Georgescu et Ionescu), with three chloroplast microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus. Although our molecular data failed to support circumscription of studied infraspecific taxa, we found that genetic patterns at both genomes are in accordance with those found previously in westward populations of this species, that is - exceptionally high levels of genetic diversity (H-T = 0.949) and low genetic differentiation (G(ST) = 0.024) at the cpDNA level, and moderate levels of genetic diversity (H-T = 0.357) and genetic differentiation (G(ST) = 0.358) at the mtDNA level. Based on genealogical relations of mtDNA types currently present in Balkans' and Iberian/African populations, we inferred that the ancestral gene pool of P. nigra already harbored polymorphism at position 328 prior to the divergence to two lineages currently present in westward and eastward parts of the species range distribution. Subsequent occurrence of three mutations, which distinguish these two lineages, suggests their long-term isolation.",
publisher = "Finnish Soc Forest Science-Natural Resources Inst Finland, Vantaa",
journal = "Silva Fennica",
title = "Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data",
number = "5",
volume = "49",
doi = "10.14214/sf.1415"
}

Sarac, Z., Dodos, T., Rajcević, N., Bojović, S., Marin, P. D.,& Aleksić, J. M.. (2015). Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data. in Silva Fennica
Finnish Soc Forest Science-Natural Resources Inst Finland, Vantaa., 49(5).
https://doi.org/10.14214/sf.1415

Sarac Z, Dodos T, Rajcević N, Bojović S, Marin PD, Aleksić JM. Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data. in Silva Fennica. 2015;49(5).
doi:10.14214/sf.1415 .

Sarac, Zorica, Dodos, Tanja, Rajcević, Nemanja, Bojović, Srdjan, Marin, Petar D., Aleksić, Jelena M., "Genetic patterns in Pinus nigra from the central Balkans inferred from plastid and mitochondrial data" in Silva Fennica, 49, no. 5 (2015),
https://doi.org/10.14214/sf.1415 . .

TY  - JOUR
AU  - Sarac, Zorica
AU  - Aleksić, Jelena M.
AU  - Dodos, Tanja
AU  - Rajcević, Nemanja
AU  - Bojović, Srdjan
AU  - Marin, Petar D.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/815
AB  - Due to the current lack of nuclear microsatellites (simple sequence repeats SSRs) specifically developed for Pinus nigra, an important European coniferous species, we cross-species amplified 12 EST-SSRs (expressed sequence tagged SSRs) developed for other Pinus species in P. nigra in order to delineate loci which can be used for assessing levels of genetic diversity and genetic structuring in this species. We amplified these loci in individuals from seven populations from the central Balkans representing four recognized infraspecific taxa of P. nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica). Contrary to expectations on high transferability of EST-SSRs into related species, only three out of 12 tested loci were successfully amplified in P. nigra, but they displayed lack/low levels of polymorphism or generated multilocus amplification products. Thus, our estimates on levels of genetic diversity (HE = 0.183) and genetic differentiation (F-ST = 0.007) were based on variability of a single locus harboring four alleles only and they should be taken with cautions. Our study highlights the need for the development of high-resolution molecular markers, such as co-dominant genic or genomic SSRs or predominantly biallelic SNPs, or utilization of anonymous dominant markers, such as AFLPs, for genotyping in P. nigra.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Cross-species amplification of nuclear est-microsatellites developed for other pinus species in pinus nigra
EP  - 217
IS  - 1
SP  - 205
VL  - 47
DO  - 10.2298/GENSR1501205S
ER  - 

@article{
author = "Sarac, Zorica and Aleksić, Jelena M. and Dodos, Tanja and Rajcević, Nemanja and Bojović, Srdjan and Marin, Petar D.",
year = "2015",
abstract = "Due to the current lack of nuclear microsatellites (simple sequence repeats SSRs) specifically developed for Pinus nigra, an important European coniferous species, we cross-species amplified 12 EST-SSRs (expressed sequence tagged SSRs) developed for other Pinus species in P. nigra in order to delineate loci which can be used for assessing levels of genetic diversity and genetic structuring in this species. We amplified these loci in individuals from seven populations from the central Balkans representing four recognized infraspecific taxa of P. nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica). Contrary to expectations on high transferability of EST-SSRs into related species, only three out of 12 tested loci were successfully amplified in P. nigra, but they displayed lack/low levels of polymorphism or generated multilocus amplification products. Thus, our estimates on levels of genetic diversity (HE = 0.183) and genetic differentiation (F-ST = 0.007) were based on variability of a single locus harboring four alleles only and they should be taken with cautions. Our study highlights the need for the development of high-resolution molecular markers, such as co-dominant genic or genomic SSRs or predominantly biallelic SNPs, or utilization of anonymous dominant markers, such as AFLPs, for genotyping in P. nigra.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Cross-species amplification of nuclear est-microsatellites developed for other pinus species in pinus nigra",
pages = "217-205",
number = "1",
volume = "47",
doi = "10.2298/GENSR1501205S"
}

Sarac, Z., Aleksić, J. M., Dodos, T., Rajcević, N., Bojović, S.,& Marin, P. D.. (2015). Cross-species amplification of nuclear est-microsatellites developed for other pinus species in pinus nigra. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 47(1), 205-217.
https://doi.org/10.2298/GENSR1501205S

Sarac Z, Aleksić JM, Dodos T, Rajcević N, Bojović S, Marin PD. Cross-species amplification of nuclear est-microsatellites developed for other pinus species in pinus nigra. in Genetika-Belgrade. 2015;47(1):205-217.
doi:10.2298/GENSR1501205S .

Sarac, Zorica, Aleksić, Jelena M., Dodos, Tanja, Rajcević, Nemanja, Bojović, Srdjan, Marin, Petar D., "Cross-species amplification of nuclear est-microsatellites developed for other pinus species in pinus nigra" in Genetika-Belgrade, 47, no. 1 (2015):205-217,
https://doi.org/10.2298/GENSR1501205S . .

TY  - JOUR
AU  - Stojanović, Danilo
AU  - Aleksić, Jelena M.
AU  - Jancić, Ivan
AU  - Jancić, Radisa
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/899
AB  - Salvia officinalis (Lamiaceae), common or Dalmatian sage, is a Mediterranean aromatic and medicinal plant used in medicine since ancient times. Knowledge on current genetic patterns and genealogical history of its natural populations is required for both breeding efforts and species conservation. We used sequences of two chloroplast intergenic spacers, 3'rps16-5'trnK and rp132-trnL, from 83 individuals from eight natural populations to distinguish between anthropogenic vs natural origin of four disjunct inland populations found outside of the main Adriatic range of the species. We found seven haplotypes, high total gene diversity (H-r = 0.695) and genetic differentiation (G(ST) = 0.682), as well as a phylogeographic structure with two lineages, a sub-structured inland-Adriatic lineage (IAL, comprising inland and Adriatic sub-lineages) and a purely Adriatic lineage (PAL). All four inland and disjunct populations, which comprised the inland sub-lineage of IAL, were almost fixed for a distinct haplotype genealogically closely related to the ancestral haplotype and displayed other features of relict populations. Along with previous biogeographic data and other lines of evidence, assumptions on their anthropogenic origin were rejected. At present, a less diverse IAL (Hd = 0.426, pi = 0.00106) and a more diverse PAL (Hd = 0.403, pi = 0.00257), whose divergence was dated to the Pliocene (3.267 Mya), do not exhibit signs of recent demographic expansions and overlap on the SE Adriatic coast, a region delineated as the main glacial refugium of S. officinalis. Conservation measures accounting for the historical distinctiveness of populations and focusing on currently the most threatened populations are recommended.
PB  - Botanischer Garten & Botanische Museum Berlin-Dahlem, Berlin
T2  - Willdenowia
T1  - A Mediterranean medicinal plant in the continental Balkans: A plastid DNA-based phylogeographic survey of Salvia officinalis (Lamiaceae) and its conservation implications
EP  - 118
IS  - 1
SP  - 103
VL  - 45
DO  - 10.3372/wi.45.45112
ER  - 

@article{
author = "Stojanović, Danilo and Aleksić, Jelena M. and Jancić, Ivan and Jancić, Radisa",
year = "2015",
abstract = "Salvia officinalis (Lamiaceae), common or Dalmatian sage, is a Mediterranean aromatic and medicinal plant used in medicine since ancient times. Knowledge on current genetic patterns and genealogical history of its natural populations is required for both breeding efforts and species conservation. We used sequences of two chloroplast intergenic spacers, 3'rps16-5'trnK and rp132-trnL, from 83 individuals from eight natural populations to distinguish between anthropogenic vs natural origin of four disjunct inland populations found outside of the main Adriatic range of the species. We found seven haplotypes, high total gene diversity (H-r = 0.695) and genetic differentiation (G(ST) = 0.682), as well as a phylogeographic structure with two lineages, a sub-structured inland-Adriatic lineage (IAL, comprising inland and Adriatic sub-lineages) and a purely Adriatic lineage (PAL). All four inland and disjunct populations, which comprised the inland sub-lineage of IAL, were almost fixed for a distinct haplotype genealogically closely related to the ancestral haplotype and displayed other features of relict populations. Along with previous biogeographic data and other lines of evidence, assumptions on their anthropogenic origin were rejected. At present, a less diverse IAL (Hd = 0.426, pi = 0.00106) and a more diverse PAL (Hd = 0.403, pi = 0.00257), whose divergence was dated to the Pliocene (3.267 Mya), do not exhibit signs of recent demographic expansions and overlap on the SE Adriatic coast, a region delineated as the main glacial refugium of S. officinalis. Conservation measures accounting for the historical distinctiveness of populations and focusing on currently the most threatened populations are recommended.",
publisher = "Botanischer Garten & Botanische Museum Berlin-Dahlem, Berlin",
journal = "Willdenowia",
title = "A Mediterranean medicinal plant in the continental Balkans: A plastid DNA-based phylogeographic survey of Salvia officinalis (Lamiaceae) and its conservation implications",
pages = "118-103",
number = "1",
volume = "45",
doi = "10.3372/wi.45.45112"
}

Stojanović, D., Aleksić, J. M., Jancić, I.,& Jancić, R.. (2015). A Mediterranean medicinal plant in the continental Balkans: A plastid DNA-based phylogeographic survey of Salvia officinalis (Lamiaceae) and its conservation implications. in Willdenowia
Botanischer Garten & Botanische Museum Berlin-Dahlem, Berlin., 45(1), 103-118.
https://doi.org/10.3372/wi.45.45112

Stojanović D, Aleksić JM, Jancić I, Jancić R. A Mediterranean medicinal plant in the continental Balkans: A plastid DNA-based phylogeographic survey of Salvia officinalis (Lamiaceae) and its conservation implications. in Willdenowia. 2015;45(1):103-118.
doi:10.3372/wi.45.45112 .

Stojanović, Danilo, Aleksić, Jelena M., Jancić, Ivan, Jancić, Radisa, "A Mediterranean medicinal plant in the continental Balkans: A plastid DNA-based phylogeographic survey of Salvia officinalis (Lamiaceae) and its conservation implications" in Willdenowia, 45, no. 1 (2015):103-118,
https://doi.org/10.3372/wi.45.45112 . .

TY  - JOUR
AU  - Skondrić, Sinisa
AU  - Aleksić, Jelena M.
AU  - Lakusić, Dmitar
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/763
AB  - The taxonomically intricate Campanula lingulata complex confined to the Balkan Peninsula is reviewed using molecular and morphological data. An extensive sample of 62 individuals for phylogenetic analyses and 402 individuals for morphometric analysis from 17 populations across the species range was used. The phylogenetic analyses based on two chloroplast intergenic spacers (trnG(UCC)-trnS(GCU) and psbA-trnH) and morphological analysis based on 50 characters revealed two allopatrically distributed lineages of the C. lingulata complex that comprise individuals from the C and S Balkans, respectively. Both molecular and morphological data allowed us to re-establish C. cichoracea Sm., a species endemic to Thessaly in Greece. This species can easily be distinguished from C. lingulata s.str. by its calyx appendages hairy on the margins and adaxial side, and ovary continuously downwards hairy all over the surface. Molecular characters that can be used to distinguish these two species comprise four parsimony-informative substitutions within trnG(UCC)-trnS(GCU), and a microsatellite with a dinucleotide (AT) motif present only within the psbA-trnH region in C. lingulata. Further studies are required for resolving the taxonomic status of the remaining Macedonian and Rhodopean sub-lineages from the S Balkans.
PB  - Botanischer Garten & Botanische Museum Berlin-Dahlem, Berlin
T2  - Willdenowia
T1  - Campanula cichoracea (Campanulaceae), a neglected species from the Balkan-Carpathian C-lingulata complex as inferred from molecular and morphological characters
EP  - 96
IS  - 1
SP  - 77
VL  - 44
DO  - 10.3372/wi.44.44111
ER  - 

@article{
author = "Skondrić, Sinisa and Aleksić, Jelena M. and Lakusić, Dmitar",
year = "2014",
abstract = "The taxonomically intricate Campanula lingulata complex confined to the Balkan Peninsula is reviewed using molecular and morphological data. An extensive sample of 62 individuals for phylogenetic analyses and 402 individuals for morphometric analysis from 17 populations across the species range was used. The phylogenetic analyses based on two chloroplast intergenic spacers (trnG(UCC)-trnS(GCU) and psbA-trnH) and morphological analysis based on 50 characters revealed two allopatrically distributed lineages of the C. lingulata complex that comprise individuals from the C and S Balkans, respectively. Both molecular and morphological data allowed us to re-establish C. cichoracea Sm., a species endemic to Thessaly in Greece. This species can easily be distinguished from C. lingulata s.str. by its calyx appendages hairy on the margins and adaxial side, and ovary continuously downwards hairy all over the surface. Molecular characters that can be used to distinguish these two species comprise four parsimony-informative substitutions within trnG(UCC)-trnS(GCU), and a microsatellite with a dinucleotide (AT) motif present only within the psbA-trnH region in C. lingulata. Further studies are required for resolving the taxonomic status of the remaining Macedonian and Rhodopean sub-lineages from the S Balkans.",
publisher = "Botanischer Garten & Botanische Museum Berlin-Dahlem, Berlin",
journal = "Willdenowia",
title = "Campanula cichoracea (Campanulaceae), a neglected species from the Balkan-Carpathian C-lingulata complex as inferred from molecular and morphological characters",
pages = "96-77",
number = "1",
volume = "44",
doi = "10.3372/wi.44.44111"
}

Skondrić, S., Aleksić, J. M.,& Lakusić, D.. (2014). Campanula cichoracea (Campanulaceae), a neglected species from the Balkan-Carpathian C-lingulata complex as inferred from molecular and morphological characters. in Willdenowia
Botanischer Garten & Botanische Museum Berlin-Dahlem, Berlin., 44(1), 77-96.
https://doi.org/10.3372/wi.44.44111

Skondrić S, Aleksić JM, Lakusić D. Campanula cichoracea (Campanulaceae), a neglected species from the Balkan-Carpathian C-lingulata complex as inferred from molecular and morphological characters. in Willdenowia. 2014;44(1):77-96.
doi:10.3372/wi.44.44111 .

Skondrić, Sinisa, Aleksić, Jelena M., Lakusić, Dmitar, "Campanula cichoracea (Campanulaceae), a neglected species from the Balkan-Carpathian C-lingulata complex as inferred from molecular and morphological characters" in Willdenowia, 44, no. 1 (2014):77-96,
https://doi.org/10.3372/wi.44.44111 . .

TY  - JOUR
AU  - Smykal, Petr
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Zlatković, Bojan
AU  - Cupina, Branko
AU  - Đorđević, Vuk
AU  - Mikić, Aleksandar
AU  - Medović, Aleksandar
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/755
AB  - The development of agriculture was a key turning point in human history, a central part of which was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. First domesticated in the Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. In this study, we present a molecular analysis of ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. Four selected chloroplast DNA loci (trnSG, trnK, matK and rbcL) amplified in six fragments of 128-340 bp with a total length of 1,329 bp were successfully recovered in order to distinguish between cultivated and wild gathered pea. Based on identified mutations, the results showed that genuine aDNA was analyzed. Moreover, DNA analysis resulted in placing the ancient sample at an intermediate position between extant cultivated [Pisum sativum L. and wild P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.]. Consequently, based on a combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of colored flower and pigmented testa, similar to today's fodder pea (P. sativum subsp. sativum var. arvense (L.) Poir.), possibly of winter type. This is the first report of successful aDNA extraction and analysis from any legume species thus far. The implications for pea domestication are discussed here.
PB  - Springer, Dordrecht
T2  - Genetic Resources and Crop Evolution
T1  - A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication
EP  - 1544
IS  - 8
SP  - 1533
VL  - 61
DO  - 10.1007/s10722-014-0128-z
ER  - 

@article{
author = "Smykal, Petr and Jovanović, Živko and Stanisavljević, Nemanja and Zlatković, Bojan and Cupina, Branko and Đorđević, Vuk and Mikić, Aleksandar and Medović, Aleksandar",
year = "2014",
abstract = "The development of agriculture was a key turning point in human history, a central part of which was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. First domesticated in the Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. In this study, we present a molecular analysis of ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. Four selected chloroplast DNA loci (trnSG, trnK, matK and rbcL) amplified in six fragments of 128-340 bp with a total length of 1,329 bp were successfully recovered in order to distinguish between cultivated and wild gathered pea. Based on identified mutations, the results showed that genuine aDNA was analyzed. Moreover, DNA analysis resulted in placing the ancient sample at an intermediate position between extant cultivated [Pisum sativum L. and wild P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.]. Consequently, based on a combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of colored flower and pigmented testa, similar to today's fodder pea (P. sativum subsp. sativum var. arvense (L.) Poir.), possibly of winter type. This is the first report of successful aDNA extraction and analysis from any legume species thus far. The implications for pea domestication are discussed here.",
publisher = "Springer, Dordrecht",
journal = "Genetic Resources and Crop Evolution",
title = "A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication",
pages = "1544-1533",
number = "8",
volume = "61",
doi = "10.1007/s10722-014-0128-z"
}

Smykal, P., Jovanović, Ž., Stanisavljević, N., Zlatković, B., Cupina, B., Đorđević, V., Mikić, A.,& Medović, A.. (2014). A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication. in Genetic Resources and Crop Evolution
Springer, Dordrecht., 61(8), 1533-1544.
https://doi.org/10.1007/s10722-014-0128-z

Smykal P, Jovanović Ž, Stanisavljević N, Zlatković B, Cupina B, Đorđević V, Mikić A, Medović A. A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication. in Genetic Resources and Crop Evolution. 2014;61(8):1533-1544.
doi:10.1007/s10722-014-0128-z .

Smykal, Petr, Jovanović, Živko, Stanisavljević, Nemanja, Zlatković, Bojan, Cupina, Branko, Đorđević, Vuk, Mikić, Aleksandar, Medović, Aleksandar, "A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication" in Genetic Resources and Crop Evolution, 61, no. 8 (2014):1533-1544,
https://doi.org/10.1007/s10722-014-0128-z . .

TY  - JOUR
AU  - Lockwood, Jared D.
AU  - Aleksić, Jelena M.
AU  - Zou, Jiabin
AU  - Wang, Jing
AU  - Liu, Jianquan
AU  - Renner, Susanne S.
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/683
AB  - Studies over the past ten years have shown that the crown groups of most conifer genera are only about 15-25 Ma old. The genus Picea (spruces, Pinaceae), with around 35 species, appears to be no exception. In addition, molecular studies of co-existing spruce species have demonstrated frequent introgression. Perhaps not surprisingly therefore previous phylogenetic studies of species relationships in Picea, based mostly on plastid sequences, suffered from poor statistical support. We therefore generated mitochondria], nuclear, and further plastid DNA sequences from carefully sourced material, striking a balance between alignability with outgroups and phylogenetic signal content. Motif duplications in mitochondria] introns were treated as characters in a stochastic Dollo model; molecular clock models were calibrated with fossils; and ancestral ranges were inferred under maximum likelihood. In agreement with previous findings, Picea diverged from its sister clade 180 million years ago (Ma), and the most recent common ancestor of today's spruces dates to 28 Ma. Different from previous analyses though, we find a large Asian clade, an American clade, and a Eurasian clade. Two expansions occurred from Asia to North America and several between Asia and Europe. Chinese P. brachytyla, American P. engelmannii, and Norway spruce, P. abies, are not monophyletic, and North America has ten, not eight species. Divergence times imply that Pleistocene refugia are unlikely to be the full explanation for the relationships between the European species and their East Asian relatives. Thus, northern Norway spruce may be part of an Asian species complex that diverged from the southern Norway spruce lineage in the Upper Miocene, some 6 Ma, which can explain the deep genetic gap noted in phylogeographic studies of Norway spruce. The large effective population sizes of spruces, and incomplete lineage sorting during speciation, mean that the interspecific relationships within each of the geographic clades require further studies, especially based on genomic information and population genetic data.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Molecular Phylogenetics and Evolution
T1  - A new phylogeny for the genus Picea from plastid, mitochondrial, and nuclear sequences
EP  - 727
IS  - 3
SP  - 717
VL  - 69
DO  - 10.1016/j.ympev.2013.07.004
ER  - 

@article{
author = "Lockwood, Jared D. and Aleksić, Jelena M. and Zou, Jiabin and Wang, Jing and Liu, Jianquan and Renner, Susanne S.",
year = "2013",
abstract = "Studies over the past ten years have shown that the crown groups of most conifer genera are only about 15-25 Ma old. The genus Picea (spruces, Pinaceae), with around 35 species, appears to be no exception. In addition, molecular studies of co-existing spruce species have demonstrated frequent introgression. Perhaps not surprisingly therefore previous phylogenetic studies of species relationships in Picea, based mostly on plastid sequences, suffered from poor statistical support. We therefore generated mitochondria], nuclear, and further plastid DNA sequences from carefully sourced material, striking a balance between alignability with outgroups and phylogenetic signal content. Motif duplications in mitochondria] introns were treated as characters in a stochastic Dollo model; molecular clock models were calibrated with fossils; and ancestral ranges were inferred under maximum likelihood. In agreement with previous findings, Picea diverged from its sister clade 180 million years ago (Ma), and the most recent common ancestor of today's spruces dates to 28 Ma. Different from previous analyses though, we find a large Asian clade, an American clade, and a Eurasian clade. Two expansions occurred from Asia to North America and several between Asia and Europe. Chinese P. brachytyla, American P. engelmannii, and Norway spruce, P. abies, are not monophyletic, and North America has ten, not eight species. Divergence times imply that Pleistocene refugia are unlikely to be the full explanation for the relationships between the European species and their East Asian relatives. Thus, northern Norway spruce may be part of an Asian species complex that diverged from the southern Norway spruce lineage in the Upper Miocene, some 6 Ma, which can explain the deep genetic gap noted in phylogeographic studies of Norway spruce. The large effective population sizes of spruces, and incomplete lineage sorting during speciation, mean that the interspecific relationships within each of the geographic clades require further studies, especially based on genomic information and population genetic data.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Molecular Phylogenetics and Evolution",
title = "A new phylogeny for the genus Picea from plastid, mitochondrial, and nuclear sequences",
pages = "727-717",
number = "3",
volume = "69",
doi = "10.1016/j.ympev.2013.07.004"
}

Lockwood, J. D., Aleksić, J. M., Zou, J., Wang, J., Liu, J.,& Renner, S. S.. (2013). A new phylogeny for the genus Picea from plastid, mitochondrial, and nuclear sequences. in Molecular Phylogenetics and Evolution
Academic Press Inc Elsevier Science, San Diego., 69(3), 717-727.
https://doi.org/10.1016/j.ympev.2013.07.004

Lockwood JD, Aleksić JM, Zou J, Wang J, Liu J, Renner SS. A new phylogeny for the genus Picea from plastid, mitochondrial, and nuclear sequences. in Molecular Phylogenetics and Evolution. 2013;69(3):717-727.
doi:10.1016/j.ympev.2013.07.004 .

Lockwood, Jared D., Aleksić, Jelena M., Zou, Jiabin, Wang, Jing, Liu, Jianquan, Renner, Susanne S., "A new phylogeny for the genus Picea from plastid, mitochondrial, and nuclear sequences" in Molecular Phylogenetics and Evolution, 69, no. 3 (2013):717-727,
https://doi.org/10.1016/j.ympev.2013.07.004 . .

TY  - JOUR
AU  - Aleksić, Jelena M.
AU  - Stojanović, Danilo
AU  - Banović Đeri, Bojana
AU  - Jancić, Radisa
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/606
AB  - We report an efficient, simple, and cost-effective protocol for the isolation of genomic DNA from an aromatic medicinal plant, common sage (Salvia officinalis L.). Our modification of the standard CTAB protocol includes two polyphenol adsorbents (PVP 10 and activated charcoal), high NaCl concentrations (4 M) for removing polysaccharides, and repeated Sevag treatment to remove proteins and other carbohydrate contaminants. The mean DNA yield obtained with our Protocol 2 was 330.6 mu g DNA g(-1) of dry leaf tissue, and the absorbance ratios 260/280 and 260/230 nm averaged 1.909 and 1.894, respectively, revealing lack of contamination. PCR amplifications of one nuclear (26S rDNA) and one chloroplast (rps16-trnK) locus indicated that our DNA isolation protocol may be used in common sage and other aromatic and medicinal plants containing essential oil for molecular biologic and biotechnological studies and for population genetics, phylogeographic, and conservation surveys in which nuclear or chloroplast genomes would be studied in large numbers of individuals.
PB  - Springer/Plenum Publishers, New York
T2  - Biochemical Genetics
T1  - A Simple and Efficient DNA Isolation Method for Salvia officinalis
EP  - 892
IS  - 11-12
SP  - 881
VL  - 50
DO  - 10.1007/s10528-012-9528-y
ER  - 

@article{
author = "Aleksić, Jelena M. and Stojanović, Danilo and Banović Đeri, Bojana and Jancić, Radisa",
year = "2012",
abstract = "We report an efficient, simple, and cost-effective protocol for the isolation of genomic DNA from an aromatic medicinal plant, common sage (Salvia officinalis L.). Our modification of the standard CTAB protocol includes two polyphenol adsorbents (PVP 10 and activated charcoal), high NaCl concentrations (4 M) for removing polysaccharides, and repeated Sevag treatment to remove proteins and other carbohydrate contaminants. The mean DNA yield obtained with our Protocol 2 was 330.6 mu g DNA g(-1) of dry leaf tissue, and the absorbance ratios 260/280 and 260/230 nm averaged 1.909 and 1.894, respectively, revealing lack of contamination. PCR amplifications of one nuclear (26S rDNA) and one chloroplast (rps16-trnK) locus indicated that our DNA isolation protocol may be used in common sage and other aromatic and medicinal plants containing essential oil for molecular biologic and biotechnological studies and for population genetics, phylogeographic, and conservation surveys in which nuclear or chloroplast genomes would be studied in large numbers of individuals.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Biochemical Genetics",
title = "A Simple and Efficient DNA Isolation Method for Salvia officinalis",
pages = "892-881",
number = "11-12",
volume = "50",
doi = "10.1007/s10528-012-9528-y"
}

Aleksić, J. M., Stojanović, D., Banović Đeri, B.,& Jancić, R.. (2012). A Simple and Efficient DNA Isolation Method for Salvia officinalis. in Biochemical Genetics
Springer/Plenum Publishers, New York., 50(11-12), 881-892.
https://doi.org/10.1007/s10528-012-9528-y

Aleksić JM, Stojanović D, Banović Đeri B, Jancić R. A Simple and Efficient DNA Isolation Method for Salvia officinalis. in Biochemical Genetics. 2012;50(11-12):881-892.
doi:10.1007/s10528-012-9528-y .

Aleksić, Jelena M., Stojanović, Danilo, Banović Đeri, Bojana, Jancić, Radisa, "A Simple and Efficient DNA Isolation Method for Salvia officinalis" in Biochemical Genetics, 50, no. 11-12 (2012):881-892,
https://doi.org/10.1007/s10528-012-9528-y . .