TY  - CONF
AU  - Lončarević, B.
AU  - Lješević, M.
AU  - Joksimović, K.
AU  - Žerađanin, A.
AU  - Gojgić-Cvijović, G.
AU  - Beškoski, V.
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2174
AB  - Niska cena i jednostavna proizvodnja sintetičkih plastičnih materijala dovela je do
njihove raširene upotrebe za različita pakovanja, građevinarstvo, automobilsku i elektronsku
industriju i izradu proizvoda za domaćinstva. Zbog toga se proizvodnja plastike u poslednjih
pola veka povećala 20 puta [1]. Najviše se koristi polietilen tereftalat (PET), a procenjeno je
da je njegova proizvodnja 2022. godine dostigla 87,17 miliona kubnih metara. Visoka
otpornost PET-a, koja se ranije smatrala prednošću, danas dovodi do nekontrolisanog
nagomilavanja otpada u brojnim ekosistemima na planeti [2]. Bis-(2-hidroksietil)-tereftalat
(BHET) je komercijalni monomersa strukturom sličnom jezgru PET-a, pa se široko koristi
u ispitivanjima biodegradacije PET-a, jer je pokazano da pojedini mikroorganizmi imaju
sposobnost da ga degraduju [3]. Osim mogućnosti razgradnje različitih štetnih jedinjenja,
mikroorganizmi mogu da proizvode i egzopolisaharide (EPS), kao odgovor na različite
selektivne pritiske životne sredine. EPS su zbog svojih fizičko-hemijskih svojstava našli
primenu u farmaceutskoj, hemijskoj i prehrambenoj industriji [4].
Cilj ovog rada bio je selektovati mikroorganizme koji mogu da razgrade BHET kao
model jedinjenje za ispitivanje degradacije plastičnog otpada na bazi PET-a i sa visokim
prinosom produkuju EPS.
Sposobnost degradacije BHET-a ispitivana je na 100 bakterijskih izolata, pomoću
čvrstih podloga sa BHET-om kao glavnim izvorom ugljenika. Potom je selektovano 7 sojeva
sa najširim zonama prosvetljenja, koji su korišćeni za produkciju EPS-a u tečnim podlogama
sa saharozom. Nakon fermentacije i odvajanja biomase, EPS su taloženi dvostrukom
zapreminom etanola i njihova količina je određena gravimetrijskom metodom [5]. Masa
dobijenih EPS varirala je od 2,4 – 33,7 g/L, a odabran je soj sa visokom produkcijom za
dalje eksperimente. Pošto mikrobna produkcija polisaharida veoma zavisi od uslova
fermentacije, ispitan je uticaj pet faktora na prinos: temperature, aeracije, pH, količine
saharoze i različitih izvora azota. Rezultati su pokazali da najoptimalnija podloga za
dobijanje visokog prinosa EPS-a sadrži ekstrakt kvasca (izvor azota), 100 g/L saharoze
(izvor ugljenika), a pH vrednost joj je 7.
Budući ekperimenti će biti usmereni na ispitivanje interakcija između ključnih faktora
na proizvodnju EPS-a, radi naprednije optimizacije procesa i povećanja prinosa.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem
T1  - Egzopolisaharidi mikroorganizama koji mogu da degraduju BHET: Optimizacija produkcije
T1  - Exopolysaccharides from microorganisms which degrade BHET: Optimization of production
EP  - 46
SP  - 45
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2174
ER  - 

@conference{
author = "Lončarević, B. and Lješević, M. and Joksimović, K. and Žerađanin, A. and Gojgić-Cvijović, G. and Beškoski, V. and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Niska cena i jednostavna proizvodnja sintetičkih plastičnih materijala dovela je do
njihove raširene upotrebe za različita pakovanja, građevinarstvo, automobilsku i elektronsku
industriju i izradu proizvoda za domaćinstva. Zbog toga se proizvodnja plastike u poslednjih
pola veka povećala 20 puta [1]. Najviše se koristi polietilen tereftalat (PET), a procenjeno je
da je njegova proizvodnja 2022. godine dostigla 87,17 miliona kubnih metara. Visoka
otpornost PET-a, koja se ranije smatrala prednošću, danas dovodi do nekontrolisanog
nagomilavanja otpada u brojnim ekosistemima na planeti [2]. Bis-(2-hidroksietil)-tereftalat
(BHET) je komercijalni monomersa strukturom sličnom jezgru PET-a, pa se široko koristi
u ispitivanjima biodegradacije PET-a, jer je pokazano da pojedini mikroorganizmi imaju
sposobnost da ga degraduju [3]. Osim mogućnosti razgradnje različitih štetnih jedinjenja,
mikroorganizmi mogu da proizvode i egzopolisaharide (EPS), kao odgovor na različite
selektivne pritiske životne sredine. EPS su zbog svojih fizičko-hemijskih svojstava našli
primenu u farmaceutskoj, hemijskoj i prehrambenoj industriji [4].
Cilj ovog rada bio je selektovati mikroorganizme koji mogu da razgrade BHET kao
model jedinjenje za ispitivanje degradacije plastičnog otpada na bazi PET-a i sa visokim
prinosom produkuju EPS.
Sposobnost degradacije BHET-a ispitivana je na 100 bakterijskih izolata, pomoću
čvrstih podloga sa BHET-om kao glavnim izvorom ugljenika. Potom je selektovano 7 sojeva
sa najširim zonama prosvetljenja, koji su korišćeni za produkciju EPS-a u tečnim podlogama
sa saharozom. Nakon fermentacije i odvajanja biomase, EPS su taloženi dvostrukom
zapreminom etanola i njihova količina je određena gravimetrijskom metodom [5]. Masa
dobijenih EPS varirala je od 2,4 – 33,7 g/L, a odabran je soj sa visokom produkcijom za
dalje eksperimente. Pošto mikrobna produkcija polisaharida veoma zavisi od uslova
fermentacije, ispitan je uticaj pet faktora na prinos: temperature, aeracije, pH, količine
saharoze i različitih izvora azota. Rezultati su pokazali da najoptimalnija podloga za
dobijanje visokog prinosa EPS-a sadrži ekstrakt kvasca (izvor azota), 100 g/L saharoze
(izvor ugljenika), a pH vrednost joj je 7.
Budući ekperimenti će biti usmereni na ispitivanje interakcija između ključnih faktora
na proizvodnju EPS-a, radi naprednije optimizacije procesa i povećanja prinosa.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem",
title = "Egzopolisaharidi mikroorganizama koji mogu da degraduju BHET: Optimizacija produkcije, Exopolysaccharides from microorganisms which degrade BHET: Optimization of production",
pages = "46-45",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2174"
}

Lončarević, B., Lješević, M., Joksimović, K., Žerađanin, A., Gojgić-Cvijović, G., Beškoski, V.,& Nikodinović-Runić, J.. (2023). Egzopolisaharidi mikroorganizama koji mogu da degraduju BHET: Optimizacija produkcije. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem
Beograd : Srpsko hemijsko društvo., 9, 45-46.
https://hdl.handle.net/21.15107/rcub_imagine_2174

Lončarević B, Lješević M, Joksimović K, Žerađanin A, Gojgić-Cvijović G, Beškoski V, Nikodinović-Runić J. Egzopolisaharidi mikroorganizama koji mogu da degraduju BHET: Optimizacija produkcije. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem. 2023;9:45-46.
https://hdl.handle.net/21.15107/rcub_imagine_2174 .

Lončarević, B., Lješević, M., Joksimović, K., Žerađanin, A., Gojgić-Cvijović, G., Beškoski, V., Nikodinović-Runić, Jasmina, "Egzopolisaharidi mikroorganizama koji mogu da degraduju BHET: Optimizacija produkcije" in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem, 9 (2023):45-46,
https://hdl.handle.net/21.15107/rcub_imagine_2174 .

TY  - CONF
AU  - Lješević, M.
AU  - Lončarević, B.
AU  - Joksimović, K.
AU  - Žerađanin, A.
AU  - Pantelić, Brana
AU  - Gojgić-Cvijović, G.
AU  - Beškoski, V.
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2173
AB  - Proizvodnja plastike i zamena staklene i keramičke ambalaže plastičnim materijalima
doveli su do nagomilavanja plastičnog otpada. Neophodno je naći povoljan sistem za
degradaciju plastičnog otpada, bez nastanka toksičnih produkata ili dodatnog zagađenja
životne sredine. Polietilen-tereftalat (PET) je jedan od najčešće proizvedenih plastičnih
polimera. Proizvodnja PET-a započinje esterifikacijom tereftalne kiseline i etilen glikola,
pri čemu nastaje bis-(2-hidroksietil)-tereftalat (BHET), koji se dalje polikondenzuje do
polimera. U poslednje vreme, BHET se često koristi kao model jedinjenje za identifikovanje
novih biokatalizatora za degradaciju PET-a [1,2].
Cilj ovog rada bio je ispitivanje mehanizma degradacije BHET-a pomoću
mikroorganizama.
U preliminarnom testu na čvrstim podlogama, kapacitet za degradaciju BHET-a je
testiran kod stotinak mikroorganizama, nakon čega su odabrani najefikasniji sojevi, koji su
identifikovani sekvenciranjem gena za 16s rRNK. Dalje, ispitivana je degradacija u tečnoj
podlozi gde je BHET bio glavni izvor ugljenika. Eksperiment je trajao 7 dana, a degradacija
je praćena nakon drugog, petog i sedmog dana upotrebom tečne hromatografije (HPLC).
Kao najefikasniji sojevi pokazali su se pripadnici roda Pseudomonas. Oni su u potpunosti
transformisali BHET do različitih intermedijera.
Rezultati su pokazali da ispitivani sojevi mogu da transformišu BHET, korišćenjem
najmanje dva različita puta, pa će se naredni eksperimenti usmeriti na identifikaciju
intermedijera degradacije. Takođe, radi optimizacije degradacije, ispitivaće se simbiotsko i
sinergističko dejstvo različitih konzorcijuma, kako bi se obezbedila potpuna degradacija
ovog model jedinjenja.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
T1  - Mikrobiološka degradacija bis (2-hidroksietil)-tereftalata
T1  - Microbial degradation of bis (2-hydroxyethyl) terephthalate
EP  - 44
SP  - 43
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2173
ER  - 

@conference{
author = "Lješević, M. and Lončarević, B. and Joksimović, K. and Žerađanin, A. and Pantelić, Brana and Gojgić-Cvijović, G. and Beškoski, V. and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Proizvodnja plastike i zamena staklene i keramičke ambalaže plastičnim materijalima
doveli su do nagomilavanja plastičnog otpada. Neophodno je naći povoljan sistem za
degradaciju plastičnog otpada, bez nastanka toksičnih produkata ili dodatnog zagađenja
životne sredine. Polietilen-tereftalat (PET) je jedan od najčešće proizvedenih plastičnih
polimera. Proizvodnja PET-a započinje esterifikacijom tereftalne kiseline i etilen glikola,
pri čemu nastaje bis-(2-hidroksietil)-tereftalat (BHET), koji se dalje polikondenzuje do
polimera. U poslednje vreme, BHET se često koristi kao model jedinjenje za identifikovanje
novih biokatalizatora za degradaciju PET-a [1,2].
Cilj ovog rada bio je ispitivanje mehanizma degradacije BHET-a pomoću
mikroorganizama.
U preliminarnom testu na čvrstim podlogama, kapacitet za degradaciju BHET-a je
testiran kod stotinak mikroorganizama, nakon čega su odabrani najefikasniji sojevi, koji su
identifikovani sekvenciranjem gena za 16s rRNK. Dalje, ispitivana je degradacija u tečnoj
podlozi gde je BHET bio glavni izvor ugljenika. Eksperiment je trajao 7 dana, a degradacija
je praćena nakon drugog, petog i sedmog dana upotrebom tečne hromatografije (HPLC).
Kao najefikasniji sojevi pokazali su se pripadnici roda Pseudomonas. Oni su u potpunosti
transformisali BHET do različitih intermedijera.
Rezultati su pokazali da ispitivani sojevi mogu da transformišu BHET, korišćenjem
najmanje dva različita puta, pa će se naredni eksperimenti usmeriti na identifikaciju
intermedijera degradacije. Takođe, radi optimizacije degradacije, ispitivaće se simbiotsko i
sinergističko dejstvo različitih konzorcijuma, kako bi se obezbedila potpuna degradacija
ovog model jedinjenja.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023",
title = "Mikrobiološka degradacija bis (2-hidroksietil)-tereftalata, Microbial degradation of bis (2-hydroxyethyl) terephthalate",
pages = "44-43",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2173"
}

Lješević, M., Lončarević, B., Joksimović, K., Žerađanin, A., Pantelić, B., Gojgić-Cvijović, G., Beškoski, V.,& Nikodinović-Runić, J.. (2023). Mikrobiološka degradacija bis (2-hidroksietil)-tereftalata. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
Beograd : Srpsko hemijsko društvo., 9, 43-44.
https://hdl.handle.net/21.15107/rcub_imagine_2173

Lješević M, Lončarević B, Joksimović K, Žerađanin A, Pantelić B, Gojgić-Cvijović G, Beškoski V, Nikodinović-Runić J. Mikrobiološka degradacija bis (2-hidroksietil)-tereftalata. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023. 2023;9:43-44.
https://hdl.handle.net/21.15107/rcub_imagine_2173 .

Lješević, M., Lončarević, B., Joksimović, K., Žerađanin, A., Pantelić, Brana, Gojgić-Cvijović, G., Beškoski, V., Nikodinović-Runić, Jasmina, "Mikrobiološka degradacija bis (2-hidroksietil)-tereftalata" in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023, 9 (2023):43-44,
https://hdl.handle.net/21.15107/rcub_imagine_2173 .

TY  - JOUR
AU  - Prodić, Ivana
AU  - Krstić Ristivojević, Maja
AU  - Smiljanić, Katarina
PY  - 2023
UR  - https://www.mdpi.com/2076-3921/12/4/886
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1886
AB  - Thermally processed peanuts are ideal plant models for studying the relationship between allergenicity and antioxidant capacity of protein-rich foods, besides lipids, carbohydrates and phytochemicals. Peanut is highly praised in the human diet; however, it is rich in allergens (>75% of total proteins). One-third of peanut allergens belong to the products of genes responsible for the defence of plants against stress conditions. The proximate composition of major peanut macromolecules and polyphenols is reviewed, focusing on the identity and relative abundance of all peanut proteins derived from recent proteomic studies. The importance of thermal processing, gastrointestinal digestion (performed by INFOGEST protocol) and their influence on allergenicity and antioxidant properties of protein-rich plant food matrices is elaborated. Antioxidant properties of bioactive peptides from nuts were also considered. Moreover, there are no studies dealing simultaneously with the antioxidant and allergenic properties of protein- and polyphenol-rich foods, considering all the molecules that can significantly contribute to the antioxidant capacity during and after gastrointestinal digestion. In summary, proteins and carbohydrates are underappreciated sources of antioxidant power released during the gastrointestinal digestion of protein-rich plant foods, and it is crucial to decipher their antioxidant contribution in addition to polyphenols and vitamins before and after gastrointestinal digestion.
T2  - Antioxidants
T2  - Antioxidants
T1  - Antioxidant Properties of Protein-Rich Plant Foods in Gastrointestinal Digestion—Peanuts as Our Antioxidant Friend or Foe in Allergies
IS  - 4
SP  - 886
VL  - 12
DO  - 10.3390/antiox12040886
ER  - 

@article{
author = "Prodić, Ivana and Krstić Ristivojević, Maja and Smiljanić, Katarina",
year = "2023",
abstract = "Thermally processed peanuts are ideal plant models for studying the relationship between allergenicity and antioxidant capacity of protein-rich foods, besides lipids, carbohydrates and phytochemicals. Peanut is highly praised in the human diet; however, it is rich in allergens (>75% of total proteins). One-third of peanut allergens belong to the products of genes responsible for the defence of plants against stress conditions. The proximate composition of major peanut macromolecules and polyphenols is reviewed, focusing on the identity and relative abundance of all peanut proteins derived from recent proteomic studies. The importance of thermal processing, gastrointestinal digestion (performed by INFOGEST protocol) and their influence on allergenicity and antioxidant properties of protein-rich plant food matrices is elaborated. Antioxidant properties of bioactive peptides from nuts were also considered. Moreover, there are no studies dealing simultaneously with the antioxidant and allergenic properties of protein- and polyphenol-rich foods, considering all the molecules that can significantly contribute to the antioxidant capacity during and after gastrointestinal digestion. In summary, proteins and carbohydrates are underappreciated sources of antioxidant power released during the gastrointestinal digestion of protein-rich plant foods, and it is crucial to decipher their antioxidant contribution in addition to polyphenols and vitamins before and after gastrointestinal digestion.",
journal = "Antioxidants, Antioxidants",
title = "Antioxidant Properties of Protein-Rich Plant Foods in Gastrointestinal Digestion—Peanuts as Our Antioxidant Friend or Foe in Allergies",
number = "4",
pages = "886",
volume = "12",
doi = "10.3390/antiox12040886"
}

Prodić, I., Krstić Ristivojević, M.,& Smiljanić, K.. (2023). Antioxidant Properties of Protein-Rich Plant Foods in Gastrointestinal Digestion—Peanuts as Our Antioxidant Friend or Foe in Allergies. in Antioxidants, 12(4), 886.
https://doi.org/10.3390/antiox12040886

Prodić I, Krstić Ristivojević M, Smiljanić K. Antioxidant Properties of Protein-Rich Plant Foods in Gastrointestinal Digestion—Peanuts as Our Antioxidant Friend or Foe in Allergies. in Antioxidants. 2023;12(4):886.
doi:10.3390/antiox12040886 .

Prodić, Ivana, Krstić Ristivojević, Maja, Smiljanić, Katarina, "Antioxidant Properties of Protein-Rich Plant Foods in Gastrointestinal Digestion—Peanuts as Our Antioxidant Friend or Foe in Allergies" in Antioxidants, 12, no. 4 (2023):886,
https://doi.org/10.3390/antiox12040886 . .

TY  - CONF
AU  - Petronijević, N.
AU  - Lješević, M.
AU  - Lončarević, B.
AU  - Joksimović, K.
AU  - Žerađanin, A.
AU  - Gojgić-Cvijović, G.
AU  - Beškoski, V.
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2285
AB  - Plastika kao izvor zagađenja postaje sve veći globalni problem. Većina plastike je
dizajnirana za jednokratnu upotrebu, što dodatno povećava količinu plastičnog otpada.
Usled preopterećenja zemlje i voda plastičnim otpadom neophodno je razviti procese koji
će omogućiti efikasno uklanjanje plastike iz životne sredine [1]. Polietilen-tereftalat (PET)
spada u najkorišćenije polimere za izradu plastike, te je neophodno naći efikasan način za
njegovu degradaciju. Kao povoljan metod degradacije PET plastike predlaže se enzimska
hidroliza korišćenjem mikroorganizama koji kao izvor ugljenika mogu koristiti PET [2].
Bis-(2-hidroksietil)-tereftalat (BHET) je intermedijer u izgradnji PET polimera, te je
korišćen kao model sistem za ispitivanje degradacije PET plastike [3].
Cilj ovog rada je bila identifikacija mikroorganizama koji potencijalno razgrađuju
BHET, kao i primena čistih kultura za degradaciju istog.
Tokom studije testirana je osetljivost mikroorganizama na antibiotike (penicillin i
nalidiksinsku kiselinu). Praćena je pojava zona inhibicije 24h nakon zasejavanja
mikroorganizama. Nakon antibiograma rađeno je izolovanje DNK po protokolu za Grampozitivne
i Gram-negativne bakterije. Za svaki soj mikroorganizama umnoženi su geni za
16s rRNK lančanom reakcijom polimeraze (PCR). Rađena je preparativna agarozna
elektroforeza sa PCR produktima i DNK je ekstrahovana iz gela. Uzorci su poslati na
sekvenciranje i dobijene sekvence su spojene u programu BioEdit. Dobijene sekvence su
poređene u NCBI BLAST bazi podataka i identifikovani su rodovi izolovanih
mikroorganizama.
Ispitivana je degradacija BHET-a pomoću čistih kultura mikroorganizama, gde je
korišćena mineralna podloga, a jedan od izvora ugljenika bio je BHET. Inkubacija je trajala
14 dana, a degradacija je praćena u prvom i poslednjem danu inkubacije pomoću tankoslojne
hromatografije (TLC). Korišćen je sistem rastvarača benzen:dioksan:sirćetna kiselina
(90:25:4, v/v/v). Na slici 1 su prikazani rezultati TLC-a gde su korišćeni sojevi
Pseudomonas, Bacillus i Rhodococcus. Kao najefikasniji soj pokazao se Rhodococcus. Kako je pokazano da sami mikroorganizmi mogu da potpuno ili delimično
transformišu BHET, dalji eksperimenti će se odvijati u smeru ispitivanja potpune
degradacije pomoću konzorcijuma mikroorganizama.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
T1  - Molekularna karakterizacija mikroorganizama izolovanih iz kontaminirane životne sredine i njihova primena za degradaciju bis(2-hidroksietil) tereftalata
EP  - 134
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2285
ER  - 

@conference{
author = "Petronijević, N. and Lješević, M. and Lončarević, B. and Joksimović, K. and Žerađanin, A. and Gojgić-Cvijović, G. and Beškoski, V. and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Plastika kao izvor zagađenja postaje sve veći globalni problem. Većina plastike je
dizajnirana za jednokratnu upotrebu, što dodatno povećava količinu plastičnog otpada.
Usled preopterećenja zemlje i voda plastičnim otpadom neophodno je razviti procese koji
će omogućiti efikasno uklanjanje plastike iz životne sredine [1]. Polietilen-tereftalat (PET)
spada u najkorišćenije polimere za izradu plastike, te je neophodno naći efikasan način za
njegovu degradaciju. Kao povoljan metod degradacije PET plastike predlaže se enzimska
hidroliza korišćenjem mikroorganizama koji kao izvor ugljenika mogu koristiti PET [2].
Bis-(2-hidroksietil)-tereftalat (BHET) je intermedijer u izgradnji PET polimera, te je
korišćen kao model sistem za ispitivanje degradacije PET plastike [3].
Cilj ovog rada je bila identifikacija mikroorganizama koji potencijalno razgrađuju
BHET, kao i primena čistih kultura za degradaciju istog.
Tokom studije testirana je osetljivost mikroorganizama na antibiotike (penicillin i
nalidiksinsku kiselinu). Praćena je pojava zona inhibicije 24h nakon zasejavanja
mikroorganizama. Nakon antibiograma rađeno je izolovanje DNK po protokolu za Grampozitivne
i Gram-negativne bakterije. Za svaki soj mikroorganizama umnoženi su geni za
16s rRNK lančanom reakcijom polimeraze (PCR). Rađena je preparativna agarozna
elektroforeza sa PCR produktima i DNK je ekstrahovana iz gela. Uzorci su poslati na
sekvenciranje i dobijene sekvence su spojene u programu BioEdit. Dobijene sekvence su
poređene u NCBI BLAST bazi podataka i identifikovani su rodovi izolovanih
mikroorganizama.
Ispitivana je degradacija BHET-a pomoću čistih kultura mikroorganizama, gde je
korišćena mineralna podloga, a jedan od izvora ugljenika bio je BHET. Inkubacija je trajala
14 dana, a degradacija je praćena u prvom i poslednjem danu inkubacije pomoću tankoslojne
hromatografije (TLC). Korišćen je sistem rastvarača benzen:dioksan:sirćetna kiselina
(90:25:4, v/v/v). Na slici 1 su prikazani rezultati TLC-a gde su korišćeni sojevi
Pseudomonas, Bacillus i Rhodococcus. Kao najefikasniji soj pokazao se Rhodococcus. Kako je pokazano da sami mikroorganizmi mogu da potpuno ili delimično
transformišu BHET, dalji eksperimenti će se odvijati u smeru ispitivanja potpune
degradacije pomoću konzorcijuma mikroorganizama.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023",
title = "Molekularna karakterizacija mikroorganizama izolovanih iz kontaminirane životne sredine i njihova primena za degradaciju bis(2-hidroksietil) tereftalata",
pages = "134-133",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2285"
}

Petronijević, N., Lješević, M., Lončarević, B., Joksimović, K., Žerađanin, A., Gojgić-Cvijović, G., Beškoski, V.,& Nikodinović-Runić, J.. (2023). Molekularna karakterizacija mikroorganizama izolovanih iz kontaminirane životne sredine i njihova primena za degradaciju bis(2-hidroksietil) tereftalata. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
Beograd : Srpsko hemijsko društvo., 133-134.
https://hdl.handle.net/21.15107/rcub_imagine_2285

Petronijević N, Lješević M, Lončarević B, Joksimović K, Žerađanin A, Gojgić-Cvijović G, Beškoski V, Nikodinović-Runić J. Molekularna karakterizacija mikroorganizama izolovanih iz kontaminirane životne sredine i njihova primena za degradaciju bis(2-hidroksietil) tereftalata. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023. 2023;:133-134.
https://hdl.handle.net/21.15107/rcub_imagine_2285 .

Petronijević, N., Lješević, M., Lončarević, B., Joksimović, K., Žerađanin, A., Gojgić-Cvijović, G., Beškoski, V., Nikodinović-Runić, Jasmina, "Molekularna karakterizacija mikroorganizama izolovanih iz kontaminirane životne sredine i njihova primena za degradaciju bis(2-hidroksietil) tereftalata" in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023 (2023):133-134,
https://hdl.handle.net/21.15107/rcub_imagine_2285 .

TY  - CONF
AU  - Petronijević, Natalija
AU  - Lješević, Marija
AU  - Lončarević, Branka
AU  - Joksimović, Kristina
AU  - Gojgić-Cvijović, Gordana
AU  - Beškoski, Vladimir
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2299
AB  - Polyethylene terephthalate (PET) is a plastic material that poses a significant global concerndue to its durability and resistance to degradation. One effective method for minimizing PETwaste is through microbial degradation, resulting in the production of ethylene glycol andterephthalic acid (TA). Terephthalic acid, as a PET monomer, holds promise as a modelcompound for further exploration into PET plastic degradation and valorisation.
PB  - Institute of Technical Sciences of SASA
C3  - Twenty -first young researchers’ conference materials science and engineering
T1  - Microbial degradation of terephtalic acid as a PET-derived compound
EP  - 28
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2299
ER  - 

@conference{
author = "Petronijević, Natalija and Lješević, Marija and Lončarević, Branka and Joksimović, Kristina and Gojgić-Cvijović, Gordana and Beškoski, Vladimir and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Polyethylene terephthalate (PET) is a plastic material that poses a significant global concerndue to its durability and resistance to degradation. One effective method for minimizing PETwaste is through microbial degradation, resulting in the production of ethylene glycol andterephthalic acid (TA). Terephthalic acid, as a PET monomer, holds promise as a modelcompound for further exploration into PET plastic degradation and valorisation.",
publisher = "Institute of Technical Sciences of SASA",
journal = "Twenty -first young researchers’ conference materials science and engineering",
title = "Microbial degradation of terephtalic acid as a PET-derived compound",
pages = "28-28",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2299"
}

Petronijević, N., Lješević, M., Lončarević, B., Joksimović, K., Gojgić-Cvijović, G., Beškoski, V.,& Nikodinović-Runić, J.. (2023). Microbial degradation of terephtalic acid as a PET-derived compound. in Twenty -first young researchers’ conference materials science and engineering
Institute of Technical Sciences of SASA., 28-28.
https://hdl.handle.net/21.15107/rcub_imagine_2299

Petronijević N, Lješević M, Lončarević B, Joksimović K, Gojgić-Cvijović G, Beškoski V, Nikodinović-Runić J. Microbial degradation of terephtalic acid as a PET-derived compound. in Twenty -first young researchers’ conference materials science and engineering. 2023;:28-28.
https://hdl.handle.net/21.15107/rcub_imagine_2299 .

Petronijević, Natalija, Lješević, Marija, Lončarević, Branka, Joksimović, Kristina, Gojgić-Cvijović, Gordana, Beškoski, Vladimir, Nikodinović-Runić, Jasmina, "Microbial degradation of terephtalic acid as a PET-derived compound" in Twenty -first young researchers’ conference materials science and engineering (2023):28-28,
https://hdl.handle.net/21.15107/rcub_imagine_2299 .

TY  - JOUR
AU  - Novaković, Miroslav
AU  - Ilić-Tomić, Tatjana
AU  - Đorđević, Iris
AU  - Anđelković, Boban
AU  - Tesević, Vele
AU  - Milosavljević, Slobodan
AU  - Asakawa, Yoshinori
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0031942223001358
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1862
AB  - Bisbibenzyls are specialized metabolites found exclusively in liverworts, until recently; they represent chemical markers of liverworts. Their occurrence in vascular plants was noticed in 2007, when they were found in Primula veris subsp. macrocalyx from Russia. This report prompted us to chemically analyze the two most common Serbian Primula species, P. veris subsp. columnae and P. acaulis, in order to determine the presence of bisbibenzyls in them. Our study revealed nine structurally distinct bisbibenzyls (1–9), identified based on 1D and 2D NMR, IR, UV and HRESIMS data. Among them were five previously undescribed compounds (2–6). The remaining compounds found and previously described in the literature were: the bisbibenzyls riccardin C (1), isoperrottetin A (7), isoplagiochin E (8) and 11-O-demethylmarchantin I (9), as well as 4-hydroxyphenylmethylketone (10) and 4-hydroxy-3-methoxyphenylmethylketone (11). Riccardin C was the most dominant bisbibenzyl in both species studied. Previously, it was the first bisbibenzyl found in vascular plants (P. veris subsp. macrocalyx). An assessment of the cytotoxic activity of the isolated compounds against A549 lung cancer and healthy MRC5 cell lines was also the subject of our study. Compounds 6 and 9 exhibited significant cytotoxic activity expressed by IC50 values of 12 μM, but the selectivity was not satisfactory.
T2  - Phytochemistry
T2  - PhytochemistryPhytochemistry
T1  - Bisbibenzyls from Serbian Primula veris subsp. Columnae (Ten.) Lȕdi and P. acaulis (L.) L
SP  - 113719
VL  - 212
DO  - 10.1016/j.phytochem.2023.113719
ER  - 

@article{
author = "Novaković, Miroslav and Ilić-Tomić, Tatjana and Đorđević, Iris and Anđelković, Boban and Tesević, Vele and Milosavljević, Slobodan and Asakawa, Yoshinori",
year = "2023",
abstract = "Bisbibenzyls are specialized metabolites found exclusively in liverworts, until recently; they represent chemical markers of liverworts. Their occurrence in vascular plants was noticed in 2007, when they were found in Primula veris subsp. macrocalyx from Russia. This report prompted us to chemically analyze the two most common Serbian Primula species, P. veris subsp. columnae and P. acaulis, in order to determine the presence of bisbibenzyls in them. Our study revealed nine structurally distinct bisbibenzyls (1–9), identified based on 1D and 2D NMR, IR, UV and HRESIMS data. Among them were five previously undescribed compounds (2–6). The remaining compounds found and previously described in the literature were: the bisbibenzyls riccardin C (1), isoperrottetin A (7), isoplagiochin E (8) and 11-O-demethylmarchantin I (9), as well as 4-hydroxyphenylmethylketone (10) and 4-hydroxy-3-methoxyphenylmethylketone (11). Riccardin C was the most dominant bisbibenzyl in both species studied. Previously, it was the first bisbibenzyl found in vascular plants (P. veris subsp. macrocalyx). An assessment of the cytotoxic activity of the isolated compounds against A549 lung cancer and healthy MRC5 cell lines was also the subject of our study. Compounds 6 and 9 exhibited significant cytotoxic activity expressed by IC50 values of 12 μM, but the selectivity was not satisfactory.",
journal = "Phytochemistry, PhytochemistryPhytochemistry",
title = "Bisbibenzyls from Serbian Primula veris subsp. Columnae (Ten.) Lȕdi and P. acaulis (L.) L",
pages = "113719",
volume = "212",
doi = "10.1016/j.phytochem.2023.113719"
}

Novaković, M., Ilić-Tomić, T., Đorđević, I., Anđelković, B., Tesević, V., Milosavljević, S.,& Asakawa, Y.. (2023). Bisbibenzyls from Serbian Primula veris subsp. Columnae (Ten.) Lȕdi and P. acaulis (L.) L. in Phytochemistry, 212, 113719.
https://doi.org/10.1016/j.phytochem.2023.113719

Novaković M, Ilić-Tomić T, Đorđević I, Anđelković B, Tesević V, Milosavljević S, Asakawa Y. Bisbibenzyls from Serbian Primula veris subsp. Columnae (Ten.) Lȕdi and P. acaulis (L.) L. in Phytochemistry. 2023;212:113719.
doi:10.1016/j.phytochem.2023.113719 .

Novaković, Miroslav, Ilić-Tomić, Tatjana, Đorđević, Iris, Anđelković, Boban, Tesević, Vele, Milosavljević, Slobodan, Asakawa, Yoshinori, "Bisbibenzyls from Serbian Primula veris subsp. Columnae (Ten.) Lȕdi and P. acaulis (L.) L" in Phytochemistry, 212 (2023):113719,
https://doi.org/10.1016/j.phytochem.2023.113719 . .

TY  - CONF
AU  - Prodić, Ivana
AU  - Đukić, Teodora
AU  - Jovanović, Vesna
AU  - Smiljanić, Katarina
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2052
AB  - Porcine-derived trypsin generated proteomic data of the major peanut allergen Ara h 1
from the peanut was reassessed to search for possible facilitating/hindrance effects on
trypsin digestion efficacy caused by post-translational and chemical modifications (PTMs)
positioned on arginine or lysine (K/R) residues. If the potential effects caused by PTMs are
observed with porcine trypsin, they can be just augmented and more pronounced within
human intestinal digestion. The reasoning is in inferior performance of human trypsin
compared to porcine-derived used in proteomic digestion protocols, also in the lower
trypsin-to-sample ratio and much shorter digestion times, even though gastric digestion
precedes and trypsin is not the sole digestive enzyme.
A novel method was developed to decipher cleavage or miscleavage outcomes at scissile
bonds in each, modified and unmodified sequence counterparts, using PEAKS Studio-X+
(Bioinformatics Solutions Inc., Ontario, Canada) in the reassessment of high-resolution
tandem mass spectrometry data, from 18-hour long trypsin digestion proteomic protocols.
In general, eight site-specific and modified K/R residues with methylation, dihydroxy
and formylation showed significantly higher content of miscleaved bonds (at least >10%)
compared to their unmodified counterpart peptides. Specifically, dihydroxylation and
formylation hindered trypsin efficacy, while methylation on several K/R showed opposite
effects.
It is essential to elucidate the specific impacts of modifications on trypsin digestion
performance and if there are additional effects generated by food processing, which could
influence digestion outcomes and allergenicity of food proteins/peptides.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - The use of tryptic food protein digests data in public proteomic repositories to assess the effects of chemical and post-translational modifications on digestion outcomes
EP  - 107
SP  - 107
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2052
ER  - 

@conference{
author = "Prodić, Ivana and Đukić, Teodora and Jovanović, Vesna and Smiljanić, Katarina",
year = "2023",
abstract = "Porcine-derived trypsin generated proteomic data of the major peanut allergen Ara h 1
from the peanut was reassessed to search for possible facilitating/hindrance effects on
trypsin digestion efficacy caused by post-translational and chemical modifications (PTMs)
positioned on arginine or lysine (K/R) residues. If the potential effects caused by PTMs are
observed with porcine trypsin, they can be just augmented and more pronounced within
human intestinal digestion. The reasoning is in inferior performance of human trypsin
compared to porcine-derived used in proteomic digestion protocols, also in the lower
trypsin-to-sample ratio and much shorter digestion times, even though gastric digestion
precedes and trypsin is not the sole digestive enzyme.
A novel method was developed to decipher cleavage or miscleavage outcomes at scissile
bonds in each, modified and unmodified sequence counterparts, using PEAKS Studio-X+
(Bioinformatics Solutions Inc., Ontario, Canada) in the reassessment of high-resolution
tandem mass spectrometry data, from 18-hour long trypsin digestion proteomic protocols.
In general, eight site-specific and modified K/R residues with methylation, dihydroxy
and formylation showed significantly higher content of miscleaved bonds (at least >10%)
compared to their unmodified counterpart peptides. Specifically, dihydroxylation and
formylation hindered trypsin efficacy, while methylation on several K/R showed opposite
effects.
It is essential to elucidate the specific impacts of modifications on trypsin digestion
performance and if there are additional effects generated by food processing, which could
influence digestion outcomes and allergenicity of food proteins/peptides.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "The use of tryptic food protein digests data in public proteomic repositories to assess the effects of chemical and post-translational modifications on digestion outcomes",
pages = "107-107",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2052"
}

Prodić, I., Đukić, T., Jovanović, V.,& Smiljanić, K.. (2023). The use of tryptic food protein digests data in public proteomic repositories to assess the effects of chemical and post-translational modifications on digestion outcomes. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 107-107.
https://hdl.handle.net/21.15107/rcub_imagine_2052

Prodić I, Đukić T, Jovanović V, Smiljanić K. The use of tryptic food protein digests data in public proteomic repositories to assess the effects of chemical and post-translational modifications on digestion outcomes. in 4th Belgrade Bioinformatics Conference. 2023;4:107-107.
https://hdl.handle.net/21.15107/rcub_imagine_2052 .

Prodić, Ivana, Đukić, Teodora, Jovanović, Vesna, Smiljanić, Katarina, "The use of tryptic food protein digests data in public proteomic repositories to assess the effects of chemical and post-translational modifications on digestion outcomes" in 4th Belgrade Bioinformatics Conference, 4 (2023):107-107,
https://hdl.handle.net/21.15107/rcub_imagine_2052 .

TY  - JOUR
AU  - Slavić, Marinela Šokarda
AU  - Kojić, Milan
AU  - Margetić, Aleksandra
AU  - Stanisavljević, Nemanja
AU  - Gardijan, Lazar
AU  - Božić, Nataša
AU  - Vujčić, Zoran
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0141813023029501
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2066
AB  - α-Amylase from the thermophilic bacterial strain Anoxybacillus vranjensis ST4 (AVA) was cloned into the pMALc5HisEk expression vector and successfully expressed and purified from the Escherichia coli ER2523 host strain. AVA belongs to the GH13_5 subfamily of glycoside hydrolases and has 7 conserved sequence regions (CSRs) distributed in three distinct domains (A, B, C). In addition, there is a starch binding domain (SBD) from the CBM20 family of carbohydrate binding modules (CBMs). AVA is a monomer of 66 kDa that achieves maximum activity at 60–80 °C and is active and stable over a wide pH range (4.0–9.0). AVA retained 50 % of its activity after 31 h of incubation at 60 °C and was resistant to a large number of denaturing agents. It hydrolyzed starch granules very efficiently, releasing maltose, maltotriose and maltopentaose as the main products. The hydrolysis rates of raw corn, wheat, horseradish, and potato starch, at a concentration of 10 %, were 87.8, 85.9, 93.0, and 58 %, respectively, at pH 8.5 over a 3 h period. This study showed that the high level of expression as well as the properties of this highly stable and versatile enzyme show all the prerequisites for successful application in industry.
T2  - International Journal of Biological Macromolecules
T1  - Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications
SP  - 126055
VL  - 249
DO  - 10.1016/j.ijbiomac.2023.126055
ER  - 

@article{
author = "Slavić, Marinela Šokarda and Kojić, Milan and Margetić, Aleksandra and Stanisavljević, Nemanja and Gardijan, Lazar and Božić, Nataša and Vujčić, Zoran",
year = "2023",
abstract = "α-Amylase from the thermophilic bacterial strain Anoxybacillus vranjensis ST4 (AVA) was cloned into the pMALc5HisEk expression vector and successfully expressed and purified from the Escherichia coli ER2523 host strain. AVA belongs to the GH13_5 subfamily of glycoside hydrolases and has 7 conserved sequence regions (CSRs) distributed in three distinct domains (A, B, C). In addition, there is a starch binding domain (SBD) from the CBM20 family of carbohydrate binding modules (CBMs). AVA is a monomer of 66 kDa that achieves maximum activity at 60–80 °C and is active and stable over a wide pH range (4.0–9.0). AVA retained 50 % of its activity after 31 h of incubation at 60 °C and was resistant to a large number of denaturing agents. It hydrolyzed starch granules very efficiently, releasing maltose, maltotriose and maltopentaose as the main products. The hydrolysis rates of raw corn, wheat, horseradish, and potato starch, at a concentration of 10 %, were 87.8, 85.9, 93.0, and 58 %, respectively, at pH 8.5 over a 3 h period. This study showed that the high level of expression as well as the properties of this highly stable and versatile enzyme show all the prerequisites for successful application in industry.",
journal = "International Journal of Biological Macromolecules",
title = "Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications",
pages = "126055",
volume = "249",
doi = "10.1016/j.ijbiomac.2023.126055"
}

Slavić, M. Š., Kojić, M., Margetić, A., Stanisavljević, N., Gardijan, L., Božić, N.,& Vujčić, Z.. (2023). Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications. in International Journal of Biological Macromolecules, 249, 126055.
https://doi.org/10.1016/j.ijbiomac.2023.126055

Slavić MŠ, Kojić M, Margetić A, Stanisavljević N, Gardijan L, Božić N, Vujčić Z. Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications. in International Journal of Biological Macromolecules. 2023;249:126055.
doi:10.1016/j.ijbiomac.2023.126055 .

Slavić, Marinela Šokarda, Kojić, Milan, Margetić, Aleksandra, Stanisavljević, Nemanja, Gardijan, Lazar, Božić, Nataša, Vujčić, Zoran, "Highly stable and versatile α-amylase from Anoxybacillus vranjensis ST4 suitable for various applications" in International Journal of Biological Macromolecules, 249 (2023):126055,
https://doi.org/10.1016/j.ijbiomac.2023.126055 . .

TY  - CONF
AU  - Savić, Aleksa
AU  - Vidović, Marija
AU  - Radosavljević, Jelena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1854
AB  - Fluorescentno obeleženi proteini su neprocenjivi alati u laboratorijskoj praksi za in vivo lokalizovanje i ispitivanje interakcija proteina. Dizajnirali smo vektor za ekspresiju mCerulean3 sa N-terminalnim heksahistidinskim obeleživacem fuzionisanim preko poliasparaginskog linkera i proteolitickog mesta za proteazu virusa graviranosti duvana (TEV) sa -sinukleinom. Ovaj konstrukt može se upotrebiti za proizvodnju -sinukleina nativne sekvence nakon proteolize TEV proteazom. Gen za mCerulean3 je nizom lancanih reakcija polimeraze (SOEing PCR) fuzionisan sa genom za -sinuklein i nakon amplifikacije ukloniran u plazmid pDUET-1. Escherichia coli BL21(DE3) je, nakon transformacije ovim konstruktom, upotrebljena za proizvodnju himernog proteina koji je zadržao fluorescentna svojstva sa prinosom od ~2 mg po litru medijuma nakon precišcavanja imobilizovanom metal-afinitetnom hromatografijom (elektroforetska cistoca: ~80%). Ovaj himerni protein je uspešno proteolizovan TEV proteazom.
AB  - Fluorescently labeled proteins are invaluable tools in laboratory practice to assess the in vivo localization and the interactions of proteins. Here we have designed an expression vector with an N-terminal hexahistidine-tagged mCerulean3 fused through a polyasparagine linker and the proteolytic site of tobacco etch virus protease (TEV) to - synuclein. This construct can be used to produce -synuclein of a native sequence after proteolysis with TEV protease. After fusion of the genes for mCerulean3 and -synuclein through a series of polymerase chain reactions (SOEing PCR), the resulting gene for the chimeric protein was cloned into the pDUET-1 plasmid. Escherichia coli BL21(DE3), upon transformation with this construct, can be used to produce the chimeric protein that retained the fluorescent properties of mCerulean3, with a yield of ~2 mg per liter of medium after purification by immobilized metal-affinity chromatography (electrophoretic purity: ~80%). This chimeric protein was successfully proteolyzed by TEV protease.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine
T1  - Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli
T1  - Cloning and expression of fluorescently labeled )-synuclein in Eschierichia coli
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1854
ER  - 

@conference{
author = "Savić, Aleksa and Vidović, Marija and Radosavljević, Jelena",
year = "2022",
abstract = "Fluorescentno obeleženi proteini su neprocenjivi alati u laboratorijskoj praksi za in vivo lokalizovanje i ispitivanje interakcija proteina. Dizajnirali smo vektor za ekspresiju mCerulean3 sa N-terminalnim heksahistidinskim obeleživacem fuzionisanim preko poliasparaginskog linkera i proteolitickog mesta za proteazu virusa graviranosti duvana (TEV) sa -sinukleinom. Ovaj konstrukt može se upotrebiti za proizvodnju -sinukleina nativne sekvence nakon proteolize TEV proteazom. Gen za mCerulean3 je nizom lancanih reakcija polimeraze (SOEing PCR) fuzionisan sa genom za -sinuklein i nakon amplifikacije ukloniran u plazmid pDUET-1. Escherichia coli BL21(DE3) je, nakon transformacije ovim konstruktom, upotrebljena za proizvodnju himernog proteina koji je zadržao fluorescentna svojstva sa prinosom od ~2 mg po litru medijuma nakon precišcavanja imobilizovanom metal-afinitetnom hromatografijom (elektroforetska cistoca: ~80%). Ovaj himerni protein je uspešno proteolizovan TEV proteazom., Fluorescently labeled proteins are invaluable tools in laboratory practice to assess the in vivo localization and the interactions of proteins. Here we have designed an expression vector with an N-terminal hexahistidine-tagged mCerulean3 fused through a polyasparagine linker and the proteolytic site of tobacco etch virus protease (TEV) to - synuclein. This construct can be used to produce -synuclein of a native sequence after proteolysis with TEV protease. After fusion of the genes for mCerulean3 and -synuclein through a series of polymerase chain reactions (SOEing PCR), the resulting gene for the chimeric protein was cloned into the pDUET-1 plasmid. Escherichia coli BL21(DE3), upon transformation with this construct, can be used to produce the chimeric protein that retained the fluorescent properties of mCerulean3, with a yield of ~2 mg per liter of medium after purification by immobilized metal-affinity chromatography (electrophoretic purity: ~80%). This chimeric protein was successfully proteolyzed by TEV protease.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine",
title = "Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli, Cloning and expression of fluorescently labeled )-synuclein in Eschierichia coli",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1854"
}

Savić, A., Vidović, M.,& Radosavljević, J.. (2022). Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli. in 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine
Beograd : Srpsko hemijsko društvo..
https://hdl.handle.net/21.15107/rcub_imagine_1854

Savić A, Vidović M, Radosavljević J. Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli. in 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine. 2022;.
https://hdl.handle.net/21.15107/rcub_imagine_1854 .

Savić, Aleksa, Vidović, Marija, Radosavljević, Jelena, "Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli" in 58. Savetovanje Srpskog hemijskog društva, Beograd 9. i 10. jun 2022. godine (2022),
https://hdl.handle.net/21.15107/rcub_imagine_1854 .

TY  - CONF
AU  - Savić, Aleksa D.
AU  - Vidović, Marija
AU  - Radosavljević, Jelena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1832
AB  - Fluorescentno obeleženi proteini su neprocenjivi alati u laboratorijskoj praksi za in vivo lokalizovanje i ispitivanje interakcija proteina. Dizajnirali smo vektor za ekspresiju mCerulean3 sa N-terminalnim heksahistidinskim obeleživacem fuzionisanim preko poliasparaginskog linkera i proteolitickog mesta za proteazu virusa graviranosti duvana (TEV) sa -sinukleinom. Ovaj konstrukt može se upotrebiti za proizvodnju -sinukleina nativne sekvence nakon proteolize TEV proteazom. Gen za mCerulean3 je nizom lancanih reakcija polimeraze (SOEing PCR) fuzionisan sa genom za -sinuklein i nakon amplifikacije ukloniran u plazmid pDUET-1. Escherichia coli BL21(DE3) je, nakon transformacije ovim konstruktom, upotrebljena za proizvodnju himernog proteina koji je zadržao fluorescentna svojstva sa prinosom od ~2 mg po litru medijuma nakon precišcavanja imobilizovanom metal-afinitetnom hromatografijom (elektroforetska cistoca: ~80%). Ovaj himerni protein je uspešno proteolizovan TEV proteazom.
AB  - Fluorescently labeled proteins are invaluable tools in laboratory practice to assess the in vivo localization and the interactions of proteins. Here we have designed an expression vector with an N-terminal hexahistidine-tagged mCerulean3 fused through a polyasparagine linker and the proteolytic site of tobacco etch virus protease (TEV) to - synuclein. This construct can be used to produce -synuclein of a native sequence after proteolysis with TEV protease. After fusion of the genes for mCerulean3 and -synuclein through a series of polymerase chain reactions (SOEing PCR), the resulting gene for the chimeric protein was cloned into the pDUET-1 plasmid. Escherichia coli BL21(DE3), upon transformation with this construct, can be used to produce the chimeric protein that retained the fluorescent properties of mCerulean3, with a yield of ~2 mg per liter of medium after purification by immobilized metal-affinity chromatography (electrophoretic purity: ~80%). This chimeric protein was successfully proteolyzed by TEV protease.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 58. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, Beograd 9. i 10. jun 2022. godine
T1  - Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli
T1  - Cloning and expression of fluorescently labeled )-synuclein in Eschierichia coli
EP  - 68
SP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1832
ER  - 

@conference{
author = "Savić, Aleksa D. and Vidović, Marija and Radosavljević, Jelena",
year = "2022",
abstract = "Fluorescentno obeleženi proteini su neprocenjivi alati u laboratorijskoj praksi za in vivo lokalizovanje i ispitivanje interakcija proteina. Dizajnirali smo vektor za ekspresiju mCerulean3 sa N-terminalnim heksahistidinskim obeleživacem fuzionisanim preko poliasparaginskog linkera i proteolitickog mesta za proteazu virusa graviranosti duvana (TEV) sa -sinukleinom. Ovaj konstrukt može se upotrebiti za proizvodnju -sinukleina nativne sekvence nakon proteolize TEV proteazom. Gen za mCerulean3 je nizom lancanih reakcija polimeraze (SOEing PCR) fuzionisan sa genom za -sinuklein i nakon amplifikacije ukloniran u plazmid pDUET-1. Escherichia coli BL21(DE3) je, nakon transformacije ovim konstruktom, upotrebljena za proizvodnju himernog proteina koji je zadržao fluorescentna svojstva sa prinosom od ~2 mg po litru medijuma nakon precišcavanja imobilizovanom metal-afinitetnom hromatografijom (elektroforetska cistoca: ~80%). Ovaj himerni protein je uspešno proteolizovan TEV proteazom., Fluorescently labeled proteins are invaluable tools in laboratory practice to assess the in vivo localization and the interactions of proteins. Here we have designed an expression vector with an N-terminal hexahistidine-tagged mCerulean3 fused through a polyasparagine linker and the proteolytic site of tobacco etch virus protease (TEV) to - synuclein. This construct can be used to produce -synuclein of a native sequence after proteolysis with TEV protease. After fusion of the genes for mCerulean3 and -synuclein through a series of polymerase chain reactions (SOEing PCR), the resulting gene for the chimeric protein was cloned into the pDUET-1 plasmid. Escherichia coli BL21(DE3), upon transformation with this construct, can be used to produce the chimeric protein that retained the fluorescent properties of mCerulean3, with a yield of ~2 mg per liter of medium after purification by immobilized metal-affinity chromatography (electrophoretic purity: ~80%). This chimeric protein was successfully proteolyzed by TEV protease.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "58. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, Beograd 9. i 10. jun 2022. godine",
title = "Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli, Cloning and expression of fluorescently labeled )-synuclein in Eschierichia coli",
pages = "68-68",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1832"
}

Savić, A. D., Vidović, M.,& Radosavljević, J.. (2022). Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli. in 58. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, Beograd 9. i 10. jun 2022. godine
Beograd : Srpsko hemijsko društvo., 68-68.
https://hdl.handle.net/21.15107/rcub_imagine_1832

Savić AD, Vidović M, Radosavljević J. Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli. in 58. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, Beograd 9. i 10. jun 2022. godine. 2022;:68-68.
https://hdl.handle.net/21.15107/rcub_imagine_1832 .

Savić, Aleksa D., Vidović, Marija, Radosavljević, Jelena, "Kloniranje i ekspresija fluorescentno obeleženog )-sinukleina u bakteriji Escherichia coli" in 58. Savetovanje Srpskog hemijskog društva, Kratki izvodi radova, Beograd 9. i 10. jun 2022. godine (2022):68-68,
https://hdl.handle.net/21.15107/rcub_imagine_1832 .

TY  - CONF
AU  - Savić, Aleksa
AU  - Vidović, Marija
AU  - Radosavljević, Jelena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1831
AB  - Alpha-synuclein is an intrinsically disordered protein prone to aggregation and it is involved in the formation of brain tissue amyloids in patients with Parkinson's and other neurodegenerative diseases. By fluorescently labeling recombinantly expressed proteins, the processes of expression and purification of the same can be easily visually monitored. In a previous work, we have constructed three plasmids for the expression of α-synuclein fused C-terminally to His-tagged mCerulean3 through a polyasparagine linker and tobacco etch virus (TEV) proteolytic site. Here we have transformed these vectors into Escherichia coli BL21(DE3) and BL21(DE3)pLysS and optimized the expression conditions by response surface methodology (temperature, time after induction, concentration of induction reagent) of the chimeric proteins coded by vectors. Expression of the chimeric protein was tested at optimal conditions in different media and terrific broth gave the highest yield. The obtained chimeric protein was purified by immobilized metal-affinity chromatography (IMAC), yielding ~29 mg of chimeric protein per liter of medium and was shown to be successfully proteolyzed by TEV protease, giving a fragment of α-synuclein of expected electrophoretic mobility. After another round of IMAC, the obtained α-synuclein of native sequence was mainly found to be in the flow-through.
PB  - Serbian Chemical Society
PB  - Serbian Young Chemists’ Club
C3  - 8th Conference of Young Chemists of Serbia, 29th October 2022, Belgrade
T1  - Optimization of the expression conditions of fluorescently labeled α-synuclein in Escherichia coli by response surface methodology and proteolysis by tobacco etch virus protease
EP  - 10
SP  - 10
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1831
ER  - 

@conference{
author = "Savić, Aleksa and Vidović, Marija and Radosavljević, Jelena",
year = "2022",
abstract = "Alpha-synuclein is an intrinsically disordered protein prone to aggregation and it is involved in the formation of brain tissue amyloids in patients with Parkinson's and other neurodegenerative diseases. By fluorescently labeling recombinantly expressed proteins, the processes of expression and purification of the same can be easily visually monitored. In a previous work, we have constructed three plasmids for the expression of α-synuclein fused C-terminally to His-tagged mCerulean3 through a polyasparagine linker and tobacco etch virus (TEV) proteolytic site. Here we have transformed these vectors into Escherichia coli BL21(DE3) and BL21(DE3)pLysS and optimized the expression conditions by response surface methodology (temperature, time after induction, concentration of induction reagent) of the chimeric proteins coded by vectors. Expression of the chimeric protein was tested at optimal conditions in different media and terrific broth gave the highest yield. The obtained chimeric protein was purified by immobilized metal-affinity chromatography (IMAC), yielding ~29 mg of chimeric protein per liter of medium and was shown to be successfully proteolyzed by TEV protease, giving a fragment of α-synuclein of expected electrophoretic mobility. After another round of IMAC, the obtained α-synuclein of native sequence was mainly found to be in the flow-through.",
publisher = "Serbian Chemical Society, Serbian Young Chemists’ Club",
journal = "8th Conference of Young Chemists of Serbia, 29th October 2022, Belgrade",
title = "Optimization of the expression conditions of fluorescently labeled α-synuclein in Escherichia coli by response surface methodology and proteolysis by tobacco etch virus protease",
pages = "10-10",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1831"
}

Savić, A., Vidović, M.,& Radosavljević, J.. (2022). Optimization of the expression conditions of fluorescently labeled α-synuclein in Escherichia coli by response surface methodology and proteolysis by tobacco etch virus protease. in 8th Conference of Young Chemists of Serbia, 29th October 2022, Belgrade
Serbian Chemical Society., 10-10.
https://hdl.handle.net/21.15107/rcub_imagine_1831

Savić A, Vidović M, Radosavljević J. Optimization of the expression conditions of fluorescently labeled α-synuclein in Escherichia coli by response surface methodology and proteolysis by tobacco etch virus protease. in 8th Conference of Young Chemists of Serbia, 29th October 2022, Belgrade. 2022;:10-10.
https://hdl.handle.net/21.15107/rcub_imagine_1831 .

Savić, Aleksa, Vidović, Marija, Radosavljević, Jelena, "Optimization of the expression conditions of fluorescently labeled α-synuclein in Escherichia coli by response surface methodology and proteolysis by tobacco etch virus protease" in 8th Conference of Young Chemists of Serbia, 29th October 2022, Belgrade (2022):10-10,
https://hdl.handle.net/21.15107/rcub_imagine_1831 .

TY  - JOUR
AU  - Trifunović, Sara
AU  - Smiljanić, Katarina
AU  - Sickmann, Albert
AU  - Solari, Fiorella A.
AU  - Kolarević, Stoimir
AU  - Divac Rankov, Aleksandra
AU  - Ljujić, Mila
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1583
AB  - Background Although still considered a safer alternative to classical cigarettes, growing body of work points to harmful effects of electronic cigarettes (e-cigarettes) affecting a range of cellular processes. The biological effect of e-cigarettes needs to be investigated in more detail considering their widespread use. Methods In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, with and without nicotine. Mutagenicity was evaluated by HPRT assay, genotoxicity by comet assay and the effect on cellular communication by metabolic cooperation assay. Additionally, comprehensive proteome analysis was performed via high resolution, parallel accumulation serial fragmentation-PASEF mass spectrometry. Results E-cigarette liquid concentration used in this study showed no mutagenic or genotoxic effect, however it negatively impacted metabolic cooperation between V79 cells. Both e-cigarette liquids induced significant depletion in total number of proteins and impairment of mitochondrial function in treated cells. The focal adhesion proteins were upregulated, which is in accordance with the results of metabolic cooperation assay. Increased presence of posttranslational modifications (PTMs), including carbonylation and direct oxidative modifications, was observed. Data are available via ProteomeXchange with identifier PXD032071. Conclusions Our study revealed impairment of metabolic cooperation as well as significant proteome and PTMs alterations in V79 cells treated with e-cigarette liquid warranting future studies on e-cigarettes health impact.
PB  - BMC, London
T2  - Respiratory Research
T1  - Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells
IS  - 1
VL  - 23
DO  - 10.1186/s12931-022-02102-w
ER  - 

@article{
author = "Trifunović, Sara and Smiljanić, Katarina and Sickmann, Albert and Solari, Fiorella A. and Kolarević, Stoimir and Divac Rankov, Aleksandra and Ljujić, Mila",
year = "2022",
abstract = "Background Although still considered a safer alternative to classical cigarettes, growing body of work points to harmful effects of electronic cigarettes (e-cigarettes) affecting a range of cellular processes. The biological effect of e-cigarettes needs to be investigated in more detail considering their widespread use. Methods In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, with and without nicotine. Mutagenicity was evaluated by HPRT assay, genotoxicity by comet assay and the effect on cellular communication by metabolic cooperation assay. Additionally, comprehensive proteome analysis was performed via high resolution, parallel accumulation serial fragmentation-PASEF mass spectrometry. Results E-cigarette liquid concentration used in this study showed no mutagenic or genotoxic effect, however it negatively impacted metabolic cooperation between V79 cells. Both e-cigarette liquids induced significant depletion in total number of proteins and impairment of mitochondrial function in treated cells. The focal adhesion proteins were upregulated, which is in accordance with the results of metabolic cooperation assay. Increased presence of posttranslational modifications (PTMs), including carbonylation and direct oxidative modifications, was observed. Data are available via ProteomeXchange with identifier PXD032071. Conclusions Our study revealed impairment of metabolic cooperation as well as significant proteome and PTMs alterations in V79 cells treated with e-cigarette liquid warranting future studies on e-cigarettes health impact.",
publisher = "BMC, London",
journal = "Respiratory Research",
title = "Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells",
number = "1",
volume = "23",
doi = "10.1186/s12931-022-02102-w"
}

Trifunović, S., Smiljanić, K., Sickmann, A., Solari, F. A., Kolarević, S., Divac Rankov, A.,& Ljujić, M.. (2022). Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells. in Respiratory Research
BMC, London., 23(1).
https://doi.org/10.1186/s12931-022-02102-w

Trifunović S, Smiljanić K, Sickmann A, Solari FA, Kolarević S, Divac Rankov A, Ljujić M. Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells. in Respiratory Research. 2022;23(1).
doi:10.1186/s12931-022-02102-w .

Trifunović, Sara, Smiljanić, Katarina, Sickmann, Albert, Solari, Fiorella A., Kolarević, Stoimir, Divac Rankov, Aleksandra, Ljujić, Mila, "Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells" in Respiratory Research, 23, no. 1 (2022),
https://doi.org/10.1186/s12931-022-02102-w . .

TY  - JOUR
AU  - Marković, Sanja B.
AU  - Maciejewska, Natalia
AU  - Olszewski, Mateusz
AU  - Visnjevac, Aleksandar
AU  - Puerta, Adrian
AU  - Padron, Jose M.
AU  - Novaković, Irena
AU  - Kojić, Snežana
AU  - Fernandes, Henrique S.
AU  - Ramotowska, Sandra
AU  - Chylewska, Agnieszka
AU  - Makowski, Mariusz
AU  - Todorović, Tamara R.
AU  - Filipović, Nenad R.
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1551
AB  - The biological activity of Cd compounds has been investigated scarce since Cd has been recognized as a human carcinogen. However, the toxicity of cadmium is comparable to the toxicity of noble metals such as Pt and Pd. The paradigm of metal toxicity has been challenged suggesting that metal toxicity is not a constant property, yet it depends on many factors like the presence of appropriate ligands. Studies on anticancer activity of cadmium complexes showed that the complexation of various ligands resulted in complexes that showed better activities than approved drugs. In the present study, cadmium complexes with biologically potent thiazolyl/selenazoyl-hydrazone ligands have been prepared, and tested for their activity against different types of tumor cell models. The complexation of ligands with Cd(II) resulted in a synergistic effect. The antiproliferative activity study revealed that all complexes are more active compared to 5-fluorouracil and cisplatin. The mechanism of tumor cell growth inhibition reveal that selenium-based compounds induce cell death in T-47D (gland carci-noma) cells through apoptosis via caspase-3/7 activation. Additionally, their pro-apoptotic effect was stronger compared to etoposide and cisplatin. Nuclease activity, detected by gel electrophoresis, may be the possible mechanism of anticancer action of investigated complexes.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - European Journal of Medicinal Chemistry
T1  - Study of the anticancer potential of Cd complexes of selenazoyl-hydrazones and their sulfur isosters
VL  - 238
DO  - 10.1016/j.ejmech.2022.114449
ER  - 

@article{
author = "Marković, Sanja B. and Maciejewska, Natalia and Olszewski, Mateusz and Visnjevac, Aleksandar and Puerta, Adrian and Padron, Jose M. and Novaković, Irena and Kojić, Snežana and Fernandes, Henrique S. and Ramotowska, Sandra and Chylewska, Agnieszka and Makowski, Mariusz and Todorović, Tamara R. and Filipović, Nenad R.",
year = "2022",
abstract = "The biological activity of Cd compounds has been investigated scarce since Cd has been recognized as a human carcinogen. However, the toxicity of cadmium is comparable to the toxicity of noble metals such as Pt and Pd. The paradigm of metal toxicity has been challenged suggesting that metal toxicity is not a constant property, yet it depends on many factors like the presence of appropriate ligands. Studies on anticancer activity of cadmium complexes showed that the complexation of various ligands resulted in complexes that showed better activities than approved drugs. In the present study, cadmium complexes with biologically potent thiazolyl/selenazoyl-hydrazone ligands have been prepared, and tested for their activity against different types of tumor cell models. The complexation of ligands with Cd(II) resulted in a synergistic effect. The antiproliferative activity study revealed that all complexes are more active compared to 5-fluorouracil and cisplatin. The mechanism of tumor cell growth inhibition reveal that selenium-based compounds induce cell death in T-47D (gland carci-noma) cells through apoptosis via caspase-3/7 activation. Additionally, their pro-apoptotic effect was stronger compared to etoposide and cisplatin. Nuclease activity, detected by gel electrophoresis, may be the possible mechanism of anticancer action of investigated complexes.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "European Journal of Medicinal Chemistry",
title = "Study of the anticancer potential of Cd complexes of selenazoyl-hydrazones and their sulfur isosters",
volume = "238",
doi = "10.1016/j.ejmech.2022.114449"
}

Marković, S. B., Maciejewska, N., Olszewski, M., Visnjevac, A., Puerta, A., Padron, J. M., Novaković, I., Kojić, S., Fernandes, H. S., Ramotowska, S., Chylewska, A., Makowski, M., Todorović, T. R.,& Filipović, N. R.. (2022). Study of the anticancer potential of Cd complexes of selenazoyl-hydrazones and their sulfur isosters. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 238.
https://doi.org/10.1016/j.ejmech.2022.114449

Marković SB, Maciejewska N, Olszewski M, Visnjevac A, Puerta A, Padron JM, Novaković I, Kojić S, Fernandes HS, Ramotowska S, Chylewska A, Makowski M, Todorović TR, Filipović NR. Study of the anticancer potential of Cd complexes of selenazoyl-hydrazones and their sulfur isosters. in European Journal of Medicinal Chemistry. 2022;238.
doi:10.1016/j.ejmech.2022.114449 .

Marković, Sanja B., Maciejewska, Natalia, Olszewski, Mateusz, Visnjevac, Aleksandar, Puerta, Adrian, Padron, Jose M., Novaković, Irena, Kojić, Snežana, Fernandes, Henrique S., Ramotowska, Sandra, Chylewska, Agnieszka, Makowski, Mariusz, Todorović, Tamara R., Filipović, Nenad R., "Study of the anticancer potential of Cd complexes of selenazoyl-hydrazones and their sulfur isosters" in European Journal of Medicinal Chemistry, 238 (2022),
https://doi.org/10.1016/j.ejmech.2022.114449 . .

TY  - JOUR
AU  - Radaković, Nataša
AU  - Nikolić, Andrea
AU  - Terzić-Jovanović, Nataša
AU  - Stojković, Pavle
AU  - Stanković, Nada
AU  - Solaja, Bogdan
AU  - Opsenica, Igor
AU  - Pavić, Aleksandar
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1593
AB  - Candida albicans remains the main causal agent of candidiasis, the most common fungal infection with disturbingly high mortality rates worldwide. The limited diversity and efficacy of clinical antifungal drugs, exacerbated by emerging drug resistance, have resulted in the failure of current antifungal therapies. This imposes an urgent demand for the development of innovative strategies for effective eradication of candidal infections. While the existing clinical drugs display fungicidal or fungistatic activity, the strategy specifically targeting C. albicans filamentation, as the most important virulence trait, represents an attractive approach for overcoming the drawbacks related to clinical antifungals. The results acquired in this study revealed the significant potential of 5-aminotetrazoles as a new class of effective and safe anti-virulence agents. Moreover, these novel agents were active when applied both alone and in combination with clinically approved polyenes. Complete prevention of C. albicans morphogenetic yeast-to-hyphae transition was achieved at doses as low as 1.3 mM under conditions mimicking various filamentation-responsive stimuli in the human body, while no cardio-or hepatotoxicity was observed at doses as high as 200 mM. The treatment of C. albicans-infected zebrafish embryos with nystatin alone had low efficacy, while the combination of nystatin and selected 5aminotetrazoles prevented fungal filamentation, successfully eliminating the infection and rescuing the infected embryos from lethal disseminated candidiasis. In addition, the most potent anti-virulence 5aminotetrazole prevented C. albicans in developing the resistance to nystatin when applied in combination, keeping the fungus sensitive to the antifungal drug.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - European Journal of Medicinal Chemistry
T1  - Unraveling the anti-virulence potential and antifungal efficacy of 5-aminotetrazoles using the zebrafish model of disseminated candidiasis
VL  - 230
DO  - 10.1016/j.ejmech.2022.114137
ER  - 

@article{
author = "Radaković, Nataša and Nikolić, Andrea and Terzić-Jovanović, Nataša and Stojković, Pavle and Stanković, Nada and Solaja, Bogdan and Opsenica, Igor and Pavić, Aleksandar",
year = "2022",
abstract = "Candida albicans remains the main causal agent of candidiasis, the most common fungal infection with disturbingly high mortality rates worldwide. The limited diversity and efficacy of clinical antifungal drugs, exacerbated by emerging drug resistance, have resulted in the failure of current antifungal therapies. This imposes an urgent demand for the development of innovative strategies for effective eradication of candidal infections. While the existing clinical drugs display fungicidal or fungistatic activity, the strategy specifically targeting C. albicans filamentation, as the most important virulence trait, represents an attractive approach for overcoming the drawbacks related to clinical antifungals. The results acquired in this study revealed the significant potential of 5-aminotetrazoles as a new class of effective and safe anti-virulence agents. Moreover, these novel agents were active when applied both alone and in combination with clinically approved polyenes. Complete prevention of C. albicans morphogenetic yeast-to-hyphae transition was achieved at doses as low as 1.3 mM under conditions mimicking various filamentation-responsive stimuli in the human body, while no cardio-or hepatotoxicity was observed at doses as high as 200 mM. The treatment of C. albicans-infected zebrafish embryos with nystatin alone had low efficacy, while the combination of nystatin and selected 5aminotetrazoles prevented fungal filamentation, successfully eliminating the infection and rescuing the infected embryos from lethal disseminated candidiasis. In addition, the most potent anti-virulence 5aminotetrazole prevented C. albicans in developing the resistance to nystatin when applied in combination, keeping the fungus sensitive to the antifungal drug.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "European Journal of Medicinal Chemistry",
title = "Unraveling the anti-virulence potential and antifungal efficacy of 5-aminotetrazoles using the zebrafish model of disseminated candidiasis",
volume = "230",
doi = "10.1016/j.ejmech.2022.114137"
}

Radaković, N., Nikolić, A., Terzić-Jovanović, N., Stojković, P., Stanković, N., Solaja, B., Opsenica, I.,& Pavić, A.. (2022). Unraveling the anti-virulence potential and antifungal efficacy of 5-aminotetrazoles using the zebrafish model of disseminated candidiasis. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 230.
https://doi.org/10.1016/j.ejmech.2022.114137

Radaković N, Nikolić A, Terzić-Jovanović N, Stojković P, Stanković N, Solaja B, Opsenica I, Pavić A. Unraveling the anti-virulence potential and antifungal efficacy of 5-aminotetrazoles using the zebrafish model of disseminated candidiasis. in European Journal of Medicinal Chemistry. 2022;230.
doi:10.1016/j.ejmech.2022.114137 .

Radaković, Nataša, Nikolić, Andrea, Terzić-Jovanović, Nataša, Stojković, Pavle, Stanković, Nada, Solaja, Bogdan, Opsenica, Igor, Pavić, Aleksandar, "Unraveling the anti-virulence potential and antifungal efficacy of 5-aminotetrazoles using the zebrafish model of disseminated candidiasis" in European Journal of Medicinal Chemistry, 230 (2022),
https://doi.org/10.1016/j.ejmech.2022.114137 . .

TY  - JOUR
AU  - Assaleh, Mohamed H.
AU  - Jeremić, Sanja
AU  - Cvijeti, Ilija
AU  - Marinkovi, Aleksandar
AU  - Prlainovi, Nevena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1601
AB  - Antimicrobial-resistance (AMR) has become the greatest concern and highly challenging issue when treating nosocomial infections. The exigency to develop new potent compounds continues to increase worldwide, whereby derivatives of natural products are becoming more attractive. In the present pa-per, the microbiological assessment of a series of 12 cinnamide hydrazides, four of them completely novel, against clinically relevant pathogens has discovered several derivatives with promising in vitro activities against Acinetobacter baumannii, one of the most dreaded opportunistic pathogens in hospi-tals. The compounds were synthesized by combining one of three different natural acids (cinnamic, 4-chloro or 4-methoxy) with four monothiocarbohydrazones (MTCHs) -an important class of synthetic organic molecules. Their structure was confirmed by elemental microanalysis, as well as ATR-FTIR, H-1 and C-13 NMR spectra, with the addition of 2D NMR spectra for novel compounds. The hybrids of cinnamic acids and pyridine derivatives are particularly active compounds with the lowest MIC50 value of 10.4 mu M for p-chloro cinnamic acid and acetyl pyridine derivatives. An alignment-independent 3D QSAR model identified pharmacophoric hotspots and suggested several structural modifications that might improve the potency of this class of compounds against A. baumannii. The compounds are strong iron-chelating agents forming complexes with a stability constant between 10 7 and 10 9 . The synthesized derivatives represent a promising class of antibacterial compounds with activities comparable to the commonly used antibiotics.
PB  - Elsevier, Amsterdam
T2  - Journal of Molecular Structure
T1  - In vitro activity of novel cinnamic acids hydrazides against clinically important pathogens
VL  - 1262
DO  - 10.1016/j.molstruc.2022.133016
ER  - 

@article{
author = "Assaleh, Mohamed H. and Jeremić, Sanja and Cvijeti, Ilija and Marinkovi, Aleksandar and Prlainovi, Nevena",
year = "2022",
abstract = "Antimicrobial-resistance (AMR) has become the greatest concern and highly challenging issue when treating nosocomial infections. The exigency to develop new potent compounds continues to increase worldwide, whereby derivatives of natural products are becoming more attractive. In the present pa-per, the microbiological assessment of a series of 12 cinnamide hydrazides, four of them completely novel, against clinically relevant pathogens has discovered several derivatives with promising in vitro activities against Acinetobacter baumannii, one of the most dreaded opportunistic pathogens in hospi-tals. The compounds were synthesized by combining one of three different natural acids (cinnamic, 4-chloro or 4-methoxy) with four monothiocarbohydrazones (MTCHs) -an important class of synthetic organic molecules. Their structure was confirmed by elemental microanalysis, as well as ATR-FTIR, H-1 and C-13 NMR spectra, with the addition of 2D NMR spectra for novel compounds. The hybrids of cinnamic acids and pyridine derivatives are particularly active compounds with the lowest MIC50 value of 10.4 mu M for p-chloro cinnamic acid and acetyl pyridine derivatives. An alignment-independent 3D QSAR model identified pharmacophoric hotspots and suggested several structural modifications that might improve the potency of this class of compounds against A. baumannii. The compounds are strong iron-chelating agents forming complexes with a stability constant between 10 7 and 10 9 . The synthesized derivatives represent a promising class of antibacterial compounds with activities comparable to the commonly used antibiotics.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Molecular Structure",
title = "In vitro activity of novel cinnamic acids hydrazides against clinically important pathogens",
volume = "1262",
doi = "10.1016/j.molstruc.2022.133016"
}

Assaleh, M. H., Jeremić, S., Cvijeti, I., Marinkovi, A.,& Prlainovi, N.. (2022). In vitro activity of novel cinnamic acids hydrazides against clinically important pathogens. in Journal of Molecular Structure
Elsevier, Amsterdam., 1262.
https://doi.org/10.1016/j.molstruc.2022.133016

Assaleh MH, Jeremić S, Cvijeti I, Marinkovi A, Prlainovi N. In vitro activity of novel cinnamic acids hydrazides against clinically important pathogens. in Journal of Molecular Structure. 2022;1262.
doi:10.1016/j.molstruc.2022.133016 .

Assaleh, Mohamed H., Jeremić, Sanja, Cvijeti, Ilija, Marinkovi, Aleksandar, Prlainovi, Nevena, "In vitro activity of novel cinnamic acids hydrazides against clinically important pathogens" in Journal of Molecular Structure, 1262 (2022),
https://doi.org/10.1016/j.molstruc.2022.133016 . .

TY  - JOUR
AU  - Milenković, Ivana
AU  - Radotić, Ksenija
AU  - Despotović, Jovana
AU  - Loncarević, Branka
AU  - Ljesević, Marija
AU  - Spasić, Slađana Z.
AU  - Nikolić, Aleksandra
AU  - Beskoski, Vladimir P.
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1449
AB  - Cerium oxide nanoparticles (nCeO(2)) have widespread applications, but they can be hazardous to the environment. Some reports indicate the toxic effect of nCeO(2) on tested animals, but literature data are mainly contradictory. Coating of nCeO(2) can improve their suspension stability and change their interaction with the environment, which can consequently decrease their toxic effects. Herein, the exopolysaccharides levan and pullulan, due to their high water solubility, biocompatibility, and ability to form film, were used to coat nCeO(2). Additionally, the monosaccharide glucose was used, since it is a common material for nanoparticle coating. This is the first study investigating the impact of carbohydrate-coated nCeO(2) in comparison to uncoated nCeO(2) using different model organisms. The aim of this study was to test the acute toxicity of carbohydrate-coated nCeO(2) on the bacterium Vibrio fischeri NRRL B-11177, the crustacean Daphnia magna, and zebrafish Danio rerio. The second aim was to investigate the effects of nCeO(2) on respiration in Daphnia magna which was performed for the first time. Finally, it was important to see the relation between Ce bioaccumulation in Daphnia magna and Danio rerio and other investigated parameters. Our results revealed that the coating decreased the toxicity of nCeO(2) on Vibrio fischeri. The coating of nCeO(2) did not affect the nanoparticles' accumulation/adsorption or mortality in Daphnia magna or Danio rerio. Monitoring of respiration in Daphnia magna revealed changes in CO2 production after exposure to coated nCeO(2), while the crustacean's O-2 consumption was not affected by any of the coated nCeO(2). In summary, this study revealed that, at 200 mg L-1 uncoated and carbohydrate-coated nCeO(2) are not toxic for the tested organisms, however, the CO2 production in Daphnia magna is different when they are treated with coated and uncoated nCeO(2). The highest production was in glucose and levan-coated nCeO(2) according to their highest suspension stability. Daphnia magna (D. magna), Danio rerio (D. rerio), Vibrio fischeri (V. fischeri)
PB  - Elsevier, Amsterdam
T2  - Aquatic Toxicology
T1  - Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio
VL  - 236
DO  - 10.1016/j.aquatox.2021.105867
ER  - 

@article{
author = "Milenković, Ivana and Radotić, Ksenija and Despotović, Jovana and Loncarević, Branka and Ljesević, Marija and Spasić, Slađana Z. and Nikolić, Aleksandra and Beskoski, Vladimir P.",
year = "2021",
abstract = "Cerium oxide nanoparticles (nCeO(2)) have widespread applications, but they can be hazardous to the environment. Some reports indicate the toxic effect of nCeO(2) on tested animals, but literature data are mainly contradictory. Coating of nCeO(2) can improve their suspension stability and change their interaction with the environment, which can consequently decrease their toxic effects. Herein, the exopolysaccharides levan and pullulan, due to their high water solubility, biocompatibility, and ability to form film, were used to coat nCeO(2). Additionally, the monosaccharide glucose was used, since it is a common material for nanoparticle coating. This is the first study investigating the impact of carbohydrate-coated nCeO(2) in comparison to uncoated nCeO(2) using different model organisms. The aim of this study was to test the acute toxicity of carbohydrate-coated nCeO(2) on the bacterium Vibrio fischeri NRRL B-11177, the crustacean Daphnia magna, and zebrafish Danio rerio. The second aim was to investigate the effects of nCeO(2) on respiration in Daphnia magna which was performed for the first time. Finally, it was important to see the relation between Ce bioaccumulation in Daphnia magna and Danio rerio and other investigated parameters. Our results revealed that the coating decreased the toxicity of nCeO(2) on Vibrio fischeri. The coating of nCeO(2) did not affect the nanoparticles' accumulation/adsorption or mortality in Daphnia magna or Danio rerio. Monitoring of respiration in Daphnia magna revealed changes in CO2 production after exposure to coated nCeO(2), while the crustacean's O-2 consumption was not affected by any of the coated nCeO(2). In summary, this study revealed that, at 200 mg L-1 uncoated and carbohydrate-coated nCeO(2) are not toxic for the tested organisms, however, the CO2 production in Daphnia magna is different when they are treated with coated and uncoated nCeO(2). The highest production was in glucose and levan-coated nCeO(2) according to their highest suspension stability. Daphnia magna (D. magna), Danio rerio (D. rerio), Vibrio fischeri (V. fischeri)",
publisher = "Elsevier, Amsterdam",
journal = "Aquatic Toxicology",
title = "Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio",
volume = "236",
doi = "10.1016/j.aquatox.2021.105867"
}

Milenković, I., Radotić, K., Despotović, J., Loncarević, B., Ljesević, M., Spasić, S. Z., Nikolić, A.,& Beskoski, V. P.. (2021). Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio. in Aquatic Toxicology
Elsevier, Amsterdam., 236.
https://doi.org/10.1016/j.aquatox.2021.105867

Milenković I, Radotić K, Despotović J, Loncarević B, Ljesević M, Spasić SZ, Nikolić A, Beskoski VP. Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio. in Aquatic Toxicology. 2021;236.
doi:10.1016/j.aquatox.2021.105867 .

Milenković, Ivana, Radotić, Ksenija, Despotović, Jovana, Loncarević, Branka, Ljesević, Marija, Spasić, Slađana Z., Nikolić, Aleksandra, Beskoski, Vladimir P., "Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio" in Aquatic Toxicology, 236 (2021),
https://doi.org/10.1016/j.aquatox.2021.105867 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Soković Bajić, Svetlana
AU  - Jovanović, Živko
AU  - Matić, Ivana
AU  - Tolinački, Maja
AU  - Popović, Dušanka
AU  - Popović, Nikola
AU  - Terzić-Vidojević, Amarela
AU  - Golić, Nataša
AU  - Beskoski, Vladimir
AU  - Samardžić, Jelena
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1337
AB  - In this study, for the first time, the comprehensive analysis of antiproliferative and antioxidant activities of ramson, followed by the analysis of its associated microbiota and health-promoting effects of lactic acid bacteria (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant with a long history of use in traditional medicine due to its antimicrobial and antioxidant activity. In this study the influence of in vitro gastrointestinal digestion on the cytotoxic activity of A. ursinum extracts against human malignant cell lines was demonstrated. Seven sulfur compounds, the degradation products of thiosulfinates, including diallyl disulfide were shown to inhibit proliferation of malignant cells by inducing accumulation within G2/M phase as well as to induce apoptosis through activation of caspase-3 and mitochondrial signaling pathway. Further, the A. ursinum microbiota, particularly LAB with potential probiotic effects, was analyzed by culture-dependent method and culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The obtained results revealed that the most abundant genera were Streptococcus, Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the presence of two PFGE pulsotypes. The probiotic potential of the strain L. fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed that both strains are safe for human use, successfully survive the simulated gastrointestinal conditions, have potential to transiently colonize the gastrointestinal tract (GIT) and have a protective immunomodulatory effect, inducing the production of proinflammatory cytokine IL17 and regulatory cytokine IL10, while decreasing the production of proinflammatory cytokine IFN-gamma. In conclusion, the results of this study suggest that consumption of A. ursinum might have health-promoting properties, including anticancer effects, while L. fermentum strains isolated from A. ursinum leaves could be used as probiotics for human consumption.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix
VL  - 11
DO  - 10.3389/fmicb.2020.601616
ER  - 

@article{
author = "Stanisavljević, Nemanja and Soković Bajić, Svetlana and Jovanović, Živko and Matić, Ivana and Tolinački, Maja and Popović, Dušanka and Popović, Nikola and Terzić-Vidojević, Amarela and Golić, Nataša and Beskoski, Vladimir and Samardžić, Jelena",
year = "2020",
abstract = "In this study, for the first time, the comprehensive analysis of antiproliferative and antioxidant activities of ramson, followed by the analysis of its associated microbiota and health-promoting effects of lactic acid bacteria (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant with a long history of use in traditional medicine due to its antimicrobial and antioxidant activity. In this study the influence of in vitro gastrointestinal digestion on the cytotoxic activity of A. ursinum extracts against human malignant cell lines was demonstrated. Seven sulfur compounds, the degradation products of thiosulfinates, including diallyl disulfide were shown to inhibit proliferation of malignant cells by inducing accumulation within G2/M phase as well as to induce apoptosis through activation of caspase-3 and mitochondrial signaling pathway. Further, the A. ursinum microbiota, particularly LAB with potential probiotic effects, was analyzed by culture-dependent method and culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The obtained results revealed that the most abundant genera were Streptococcus, Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the presence of two PFGE pulsotypes. The probiotic potential of the strain L. fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed that both strains are safe for human use, successfully survive the simulated gastrointestinal conditions, have potential to transiently colonize the gastrointestinal tract (GIT) and have a protective immunomodulatory effect, inducing the production of proinflammatory cytokine IL17 and regulatory cytokine IL10, while decreasing the production of proinflammatory cytokine IFN-gamma. In conclusion, the results of this study suggest that consumption of A. ursinum might have health-promoting properties, including anticancer effects, while L. fermentum strains isolated from A. ursinum leaves could be used as probiotics for human consumption.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix",
volume = "11",
doi = "10.3389/fmicb.2020.601616"
}

Stanisavljević, N., Soković Bajić, S., Jovanović, Ž., Matić, I., Tolinački, M., Popović, D., Popović, N., Terzić-Vidojević, A., Golić, N., Beskoski, V.,& Samardžić, J.. (2020). Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 11.
https://doi.org/10.3389/fmicb.2020.601616

Stanisavljević N, Soković Bajić S, Jovanović Ž, Matić I, Tolinački M, Popović D, Popović N, Terzić-Vidojević A, Golić N, Beskoski V, Samardžić J. Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix. in Frontiers in Microbiology. 2020;11.
doi:10.3389/fmicb.2020.601616 .

Stanisavljević, Nemanja, Soković Bajić, Svetlana, Jovanović, Živko, Matić, Ivana, Tolinački, Maja, Popović, Dušanka, Popović, Nikola, Terzić-Vidojević, Amarela, Golić, Nataša, Beskoski, Vladimir, Samardžić, Jelena, "Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix" in Frontiers in Microbiology, 11 (2020),
https://doi.org/10.3389/fmicb.2020.601616 . .

TY  - JOUR
AU  - Novakovi, Miroslav M.
AU  - Ilić-Tomić, Tatjana
AU  - Tešević, Vele
AU  - Simić, Katarina
AU  - Ivanović, Stefan
AU  - Simić, Stefan
AU  - Opsenica, Igor
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1310
AB  - TheTrametes versicolorlaccase catalyzed oxidation of chalcone butein afforded four dimers of aurone sulfuretin (i.e.two regioisomeric pairs of diasteromers,1-4) as the major products. The formation of the dimers was explained by a two step process involving the initial cyclization of butein into aurone sulfuretin, followed by the combination of two molecules of sulfuretin. The coupling process occurred between the 2,10-double bond of one molecule of sulfuretin and the (3 ',4 ') catechol group of the other to yield a dimeric structure. This was confirmed by the experiment involving the laccase catalyzed oxidation of sulfuretin yielding the same dimeric bisaurones. Compounds1,3and4, were isolated using semipreparative HPLC and characterized by the detailed analysis of the NMR, MS, IR, and UV-vis data. The structure of compound2, isolated as a mixture containingca.25% of compound1,was proposed by the comparison of(1)H NMR data to compound1and by using LC-ESIMS analysis. The starting chalcone butein and the products of the biocatalytic transformation, aurone sulfuretin and sulfuretin dimers1,3and4,were evaluated for their cytotoxic and antioxidative propertiesin vitrousing a healthy human fibroblast (MRC5) cell line. The biotransformation products showed lower cytotoxicity but higher antioxidative properties. TheC. coggygriabark methanol extract rich in butein and sulfuretin was also biotransformed by laccase. The transformed extract exhibited significantly improved antioxidative activities.
PB  - Royal Soc Chemistry, Cambridge
T2  - New Journal of Chemistry
T1  - Bisaurones - enzymatic production and biological evaluation
EP  - 9655
IS  - 23
SP  - 9647
VL  - 44
DO  - 10.1039/d0nj00758g
ER  - 

@article{
author = "Novakovi, Miroslav M. and Ilić-Tomić, Tatjana and Tešević, Vele and Simić, Katarina and Ivanović, Stefan and Simić, Stefan and Opsenica, Igor and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "TheTrametes versicolorlaccase catalyzed oxidation of chalcone butein afforded four dimers of aurone sulfuretin (i.e.two regioisomeric pairs of diasteromers,1-4) as the major products. The formation of the dimers was explained by a two step process involving the initial cyclization of butein into aurone sulfuretin, followed by the combination of two molecules of sulfuretin. The coupling process occurred between the 2,10-double bond of one molecule of sulfuretin and the (3 ',4 ') catechol group of the other to yield a dimeric structure. This was confirmed by the experiment involving the laccase catalyzed oxidation of sulfuretin yielding the same dimeric bisaurones. Compounds1,3and4, were isolated using semipreparative HPLC and characterized by the detailed analysis of the NMR, MS, IR, and UV-vis data. The structure of compound2, isolated as a mixture containingca.25% of compound1,was proposed by the comparison of(1)H NMR data to compound1and by using LC-ESIMS analysis. The starting chalcone butein and the products of the biocatalytic transformation, aurone sulfuretin and sulfuretin dimers1,3and4,were evaluated for their cytotoxic and antioxidative propertiesin vitrousing a healthy human fibroblast (MRC5) cell line. The biotransformation products showed lower cytotoxicity but higher antioxidative properties. TheC. coggygriabark methanol extract rich in butein and sulfuretin was also biotransformed by laccase. The transformed extract exhibited significantly improved antioxidative activities.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "New Journal of Chemistry",
title = "Bisaurones - enzymatic production and biological evaluation",
pages = "9655-9647",
number = "23",
volume = "44",
doi = "10.1039/d0nj00758g"
}

Novakovi, M. M., Ilić-Tomić, T., Tešević, V., Simić, K., Ivanović, S., Simić, S., Opsenica, I.,& Nikodinović-Runić, J.. (2020). Bisaurones - enzymatic production and biological evaluation. in New Journal of Chemistry
Royal Soc Chemistry, Cambridge., 44(23), 9647-9655.
https://doi.org/10.1039/d0nj00758g

Novakovi MM, Ilić-Tomić T, Tešević V, Simić K, Ivanović S, Simić S, Opsenica I, Nikodinović-Runić J. Bisaurones - enzymatic production and biological evaluation. in New Journal of Chemistry. 2020;44(23):9647-9655.
doi:10.1039/d0nj00758g .

Novakovi, Miroslav M., Ilić-Tomić, Tatjana, Tešević, Vele, Simić, Katarina, Ivanović, Stefan, Simić, Stefan, Opsenica, Igor, Nikodinović-Runić, Jasmina, "Bisaurones - enzymatic production and biological evaluation" in New Journal of Chemistry, 44, no. 23 (2020):9647-9655,
https://doi.org/10.1039/d0nj00758g . .

TY  - JOUR
AU  - Ilić-Tomić, Tatjana
AU  - Tešević, Vele
AU  - Simić, Katarina
AU  - Ivanović, Stefan
AU  - Simić, Stefan
AU  - Opsenica, Igor
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1761
AB  - TheTrametes versicolorlaccase catalyzed oxidation of chalcone butein afforded four dimers of aurone sulfuretin (i.e.two regioisomeric pairs of diasteromers,1-4) as the major products. The formation of the dimers was explained by a two step process involving the initial cyclization of butein into aurone sulfuretin, followed by the combination of two molecules of sulfuretin. The coupling process occurred between the 2,10-double bond of one molecule of sulfuretin and the (3 ',4 ') catechol group of the other to yield a dimeric structure. This was confirmed by the experiment involving the laccase catalyzed oxidation of sulfuretin yielding the same dimeric bisaurones. Compounds1,3and4, were isolated using semipreparative HPLC and characterized by the detailed analysis of the NMR, MS, IR, and UV-vis data. The structure of compound2, isolated as a mixture containingca.25% of compound1,was proposed by the comparison of(1)H NMR data to compound1and by using LC-ESIMS analysis. The starting chalcone butein and the products of the biocatalytic transformation, aurone sulfuretin and sulfuretin dimers1,3and4,were evaluated for their cytotoxic and antioxidative propertiesin vitrousing a healthy human fibroblast (MRC5) cell line. The biotransformation products showed lower cytotoxicity but higher antioxidative properties. TheC. coggygriabark methanol extract rich in butein and sulfuretin was also biotransformed by laccase. The transformed extract exhibited significantly improved antioxidative activities.
PB  - Royal Soc Chemistry, Cambridge
T2  - New Journal of Chemistry
T1  - Bisaurones - enzymatic production and biological evaluation
EP  - 9655
IS  - 23
SP  - 9647
VL  - 44
DO  - 10.1039/d0nj00758g
ER  - 

@article{
author = "Ilić-Tomić, Tatjana and Tešević, Vele and Simić, Katarina and Ivanović, Stefan and Simić, Stefan and Opsenica, Igor and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "TheTrametes versicolorlaccase catalyzed oxidation of chalcone butein afforded four dimers of aurone sulfuretin (i.e.two regioisomeric pairs of diasteromers,1-4) as the major products. The formation of the dimers was explained by a two step process involving the initial cyclization of butein into aurone sulfuretin, followed by the combination of two molecules of sulfuretin. The coupling process occurred between the 2,10-double bond of one molecule of sulfuretin and the (3 ',4 ') catechol group of the other to yield a dimeric structure. This was confirmed by the experiment involving the laccase catalyzed oxidation of sulfuretin yielding the same dimeric bisaurones. Compounds1,3and4, were isolated using semipreparative HPLC and characterized by the detailed analysis of the NMR, MS, IR, and UV-vis data. The structure of compound2, isolated as a mixture containingca.25% of compound1,was proposed by the comparison of(1)H NMR data to compound1and by using LC-ESIMS analysis. The starting chalcone butein and the products of the biocatalytic transformation, aurone sulfuretin and sulfuretin dimers1,3and4,were evaluated for their cytotoxic and antioxidative propertiesin vitrousing a healthy human fibroblast (MRC5) cell line. The biotransformation products showed lower cytotoxicity but higher antioxidative properties. TheC. coggygriabark methanol extract rich in butein and sulfuretin was also biotransformed by laccase. The transformed extract exhibited significantly improved antioxidative activities.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "New Journal of Chemistry",
title = "Bisaurones - enzymatic production and biological evaluation",
pages = "9655-9647",
number = "23",
volume = "44",
doi = "10.1039/d0nj00758g"
}

Ilić-Tomić, T., Tešević, V., Simić, K., Ivanović, S., Simić, S., Opsenica, I.,& Nikodinović-Runić, J.. (2020). Bisaurones - enzymatic production and biological evaluation. in New Journal of Chemistry
Royal Soc Chemistry, Cambridge., 44(23), 9647-9655.
https://doi.org/10.1039/d0nj00758g

Ilić-Tomić T, Tešević V, Simić K, Ivanović S, Simić S, Opsenica I, Nikodinović-Runić J. Bisaurones - enzymatic production and biological evaluation. in New Journal of Chemistry. 2020;44(23):9647-9655.
doi:10.1039/d0nj00758g .

Ilić-Tomić, Tatjana, Tešević, Vele, Simić, Katarina, Ivanović, Stefan, Simić, Stefan, Opsenica, Igor, Nikodinović-Runić, Jasmina, "Bisaurones - enzymatic production and biological evaluation" in New Journal of Chemistry, 44, no. 23 (2020):9647-9655,
https://doi.org/10.1039/d0nj00758g . .