TY  - JOUR
AU  - Soković Bajić, Svetlana
AU  - Đokić, Jelena
AU  - Dinić, Miroslav
AU  - Tomić, Sergej
AU  - Popović, Nikola
AU  - Brdarić, Emilija
AU  - Golić, Nataša
AU  - Tolinački, Maja
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1318
AB  - The characterization of mechanisms involved in the positive effects of probiotic bacteria in various pathophysiological conditions is a prerogative for their safe and efficient application in biomedicine. We have investigated the immunological effects of live bacteria-free supernatant collected from GABA-producing Lactobacillus brevis BGZLS10-17 on Concanavalin A-stimulated mesenteric lymph node cells (MLNC), an in vitro model of activated immune cells. We have shown that GABA containing and GABA-free supernatant of Lactobacillus brevis BGZLS10-17 have strong immunoregulatory effects on MLNC. Further, GABA produced by this strain exhibit additional inhibitory effects on proliferation, IFN-gamma and IL-17 production by MLNC, and the expression of MHCII and CD80 on antigen presenting cells. At the other hand, GABA-containing supernatants displayed the strongest stimulatory effects on the expression of immunoregulatory molecules, such as Foxp3(+), IL-10, TGF-beta, CTLA4 and SIRP-alpha. By looking for the mechanisms of actions, we found that supernatants produced by BGZLS10-17 induce autophagy in different MLNC, such as CD4(+) and CD8(+) T lymphocytes, NK and NKT cells, as well as antigen presenting cells. Further, we showed that the stimulation of Foxp3(+), IL-10 and TGF-beta expression by BGZLS10-17 produced GABA is completely mediated by the induction of ATG5 dependent autophagy, and that other molecules in the supernatants display GABA-, ATG5-, Foxp3(+)-, IL-10- and TGF-beta- independent, immunoregulatory effects.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro
SP  - 1347
VL  - 10
DO  - 10.1038/s41598-020-58177-2
ER  - 

@article{
author = "Soković Bajić, Svetlana and Đokić, Jelena and Dinić, Miroslav and Tomić, Sergej and Popović, Nikola and Brdarić, Emilija and Golić, Nataša and Tolinački, Maja",
year = "2020",
abstract = "The characterization of mechanisms involved in the positive effects of probiotic bacteria in various pathophysiological conditions is a prerogative for their safe and efficient application in biomedicine. We have investigated the immunological effects of live bacteria-free supernatant collected from GABA-producing Lactobacillus brevis BGZLS10-17 on Concanavalin A-stimulated mesenteric lymph node cells (MLNC), an in vitro model of activated immune cells. We have shown that GABA containing and GABA-free supernatant of Lactobacillus brevis BGZLS10-17 have strong immunoregulatory effects on MLNC. Further, GABA produced by this strain exhibit additional inhibitory effects on proliferation, IFN-gamma and IL-17 production by MLNC, and the expression of MHCII and CD80 on antigen presenting cells. At the other hand, GABA-containing supernatants displayed the strongest stimulatory effects on the expression of immunoregulatory molecules, such as Foxp3(+), IL-10, TGF-beta, CTLA4 and SIRP-alpha. By looking for the mechanisms of actions, we found that supernatants produced by BGZLS10-17 induce autophagy in different MLNC, such as CD4(+) and CD8(+) T lymphocytes, NK and NKT cells, as well as antigen presenting cells. Further, we showed that the stimulation of Foxp3(+), IL-10 and TGF-beta expression by BGZLS10-17 produced GABA is completely mediated by the induction of ATG5 dependent autophagy, and that other molecules in the supernatants display GABA-, ATG5-, Foxp3(+)-, IL-10- and TGF-beta- independent, immunoregulatory effects.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro",
pages = "1347",
volume = "10",
doi = "10.1038/s41598-020-58177-2"
}

Soković Bajić, S., Đokić, J., Dinić, M., Tomić, S., Popović, N., Brdarić, E., Golić, N.,& Tolinački, M.. (2020). GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro. in Scientific Reports
Nature Publishing Group, London., 10, 1347.
https://doi.org/10.1038/s41598-020-58177-2

Soković Bajić S, Đokić J, Dinić M, Tomić S, Popović N, Brdarić E, Golić N, Tolinački M. GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro. in Scientific Reports. 2020;10:1347.
doi:10.1038/s41598-020-58177-2 .

Soković Bajić, Svetlana, Đokić, Jelena, Dinić, Miroslav, Tomić, Sergej, Popović, Nikola, Brdarić, Emilija, Golić, Nataša, Tolinački, Maja, "GABA potentiate the immunoregulatory effects of Lactobacillus brevis BGZLS10-17 via ATG5-dependent autophagy in vitro" in Scientific Reports, 10 (2020):1347,
https://doi.org/10.1038/s41598-020-58177-2 . .

TY  - JOUR
AU  - Filipović, Nenad
AU  - Veselinović, Ljiljana
AU  - Razić, Slavica
AU  - Jeremić, Sanja
AU  - Filipić, Metka
AU  - Zegura, Bojana
AU  - Tomić, Sergej
AU  - Čolić, Miodrag
AU  - Stevanović, Magdalena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1292
AB  - Poly (e-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1-4 mu m with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants.
PB  - Elsevier, Amsterdam
T2  - Materials Science & Engineering C-Materials For Biological Applications
T1  - Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles
EP  - 789
SP  - 776
VL  - 96
DO  - 10.1016/j.msec.2018.11.073
ER  - 

@article{
author = "Filipović, Nenad and Veselinović, Ljiljana and Razić, Slavica and Jeremić, Sanja and Filipić, Metka and Zegura, Bojana and Tomić, Sergej and Čolić, Miodrag and Stevanović, Magdalena",
year = "2019",
abstract = "Poly (e-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1-4 mu m with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants.",
publisher = "Elsevier, Amsterdam",
journal = "Materials Science & Engineering C-Materials For Biological Applications",
title = "Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles",
pages = "789-776",
volume = "96",
doi = "10.1016/j.msec.2018.11.073"
}

Filipović, N., Veselinović, L., Razić, S., Jeremić, S., Filipić, M., Zegura, B., Tomić, S., Čolić, M.,& Stevanović, M.. (2019). Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science & Engineering C-Materials For Biological Applications
Elsevier, Amsterdam., 96, 776-789.
https://doi.org/10.1016/j.msec.2018.11.073

Filipović N, Veselinović L, Razić S, Jeremić S, Filipić M, Zegura B, Tomić S, Čolić M, Stevanović M. Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science & Engineering C-Materials For Biological Applications. 2019;96:776-789.
doi:10.1016/j.msec.2018.11.073 .

Filipović, Nenad, Veselinović, Ljiljana, Razić, Slavica, Jeremić, Sanja, Filipić, Metka, Zegura, Bojana, Tomić, Sergej, Čolić, Miodrag, Stevanović, Magdalena, "Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles" in Materials Science & Engineering C-Materials For Biological Applications, 96 (2019):776-789,
https://doi.org/10.1016/j.msec.2018.11.073 . .

TY  - JOUR
AU  - Marković, Milan
AU  - Tomić, Sergej
AU  - Đokić, Jelena
AU  - Mihajlović, Dusan
AU  - Vucević, Dragana
AU  - Gazivoda, Dragan
AU  - Duka, Milos
AU  - Čolić, Miodrag
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1104
AB  - Background/Aim. Mesenchymal stem cells (MSCs) have been shown to suppress immune and inflammatory reactions. However, it is not known whether MSCs from inflammatory tissues, such as periapical lesions (PLs) have similar effects. This question was addressed in this study in which the aim was to examine the capacity of PL-MSCs for modulating cytokine production by local immune cells. Methods. PL-MSCs were isolated from asymptomatic (as) and symptomatic (sy) PLs. Their phenotype was analyzed by flow cytometry by detecting MSC surface markers. Anti-inflammatory and immunomodulatory properties of PL-MSCs were examined by measuring cytokine production in direct co-culture experiments with mononuclear cells (MNCs) isolated from asPLs and syPLs, respectively. The levels of cytokines in supernatants were determined by specific ELISA kits. Results. Both PL-MSCs lines were characterized by typical MSC phenotype, with the predominance of CD29, CD44, CD90, CD105 and CD166. However, the lines, independently of their similar phenotype had the same modulatory effect on cytokine production, but the response of asPL-MNCs and syPL-MNCs was different, in spite of similar composition of these MNCs. Both MSC lines inhibited the production of inflammatory cytokines, such as interleukin-10 (IL-10) and tumor necrosis factor-0 (TNF-0). However, IL-8 was only down-regulated in the co-culture of these MSC lines with syPL-MNCs. The PL-MSCs also modulated the production of immunoregulatory cytokines. Transforming growth factor-0 (TGF-0) was up-regulated by both as- and syPL-MNCs but IL-10 was up-regulated only by asPL-MNCs. Conclusion. Our results showed that PL-MSCs contribute to the restriction of local inflammatory and immune responses, but this effect is probably less efficient during the exacerbation of PL inflammation.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Mesenchymal stem cells from periapical lesions modulate cytokine production by local immune cells
EP  - 480
IS  - 5
SP  - 473
VL  - 75
DO  - 10.2298/VSP160901272M
ER  - 

@article{
author = "Marković, Milan and Tomić, Sergej and Đokić, Jelena and Mihajlović, Dusan and Vucević, Dragana and Gazivoda, Dragan and Duka, Milos and Čolić, Miodrag",
year = "2018",
abstract = "Background/Aim. Mesenchymal stem cells (MSCs) have been shown to suppress immune and inflammatory reactions. However, it is not known whether MSCs from inflammatory tissues, such as periapical lesions (PLs) have similar effects. This question was addressed in this study in which the aim was to examine the capacity of PL-MSCs for modulating cytokine production by local immune cells. Methods. PL-MSCs were isolated from asymptomatic (as) and symptomatic (sy) PLs. Their phenotype was analyzed by flow cytometry by detecting MSC surface markers. Anti-inflammatory and immunomodulatory properties of PL-MSCs were examined by measuring cytokine production in direct co-culture experiments with mononuclear cells (MNCs) isolated from asPLs and syPLs, respectively. The levels of cytokines in supernatants were determined by specific ELISA kits. Results. Both PL-MSCs lines were characterized by typical MSC phenotype, with the predominance of CD29, CD44, CD90, CD105 and CD166. However, the lines, independently of their similar phenotype had the same modulatory effect on cytokine production, but the response of asPL-MNCs and syPL-MNCs was different, in spite of similar composition of these MNCs. Both MSC lines inhibited the production of inflammatory cytokines, such as interleukin-10 (IL-10) and tumor necrosis factor-0 (TNF-0). However, IL-8 was only down-regulated in the co-culture of these MSC lines with syPL-MNCs. The PL-MSCs also modulated the production of immunoregulatory cytokines. Transforming growth factor-0 (TGF-0) was up-regulated by both as- and syPL-MNCs but IL-10 was up-regulated only by asPL-MNCs. Conclusion. Our results showed that PL-MSCs contribute to the restriction of local inflammatory and immune responses, but this effect is probably less efficient during the exacerbation of PL inflammation.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Mesenchymal stem cells from periapical lesions modulate cytokine production by local immune cells",
pages = "480-473",
number = "5",
volume = "75",
doi = "10.2298/VSP160901272M"
}

Marković, M., Tomić, S., Đokić, J., Mihajlović, D., Vucević, D., Gazivoda, D., Duka, M.,& Čolić, M.. (2018). Mesenchymal stem cells from periapical lesions modulate cytokine production by local immune cells. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 75(5), 473-480.
https://doi.org/10.2298/VSP160901272M

Marković M, Tomić S, Đokić J, Mihajlović D, Vucević D, Gazivoda D, Duka M, Čolić M. Mesenchymal stem cells from periapical lesions modulate cytokine production by local immune cells. in Vojnosanitetski pregled. 2018;75(5):473-480.
doi:10.2298/VSP160901272M .

Marković, Milan, Tomić, Sergej, Đokić, Jelena, Mihajlović, Dusan, Vucević, Dragana, Gazivoda, Dragan, Duka, Milos, Čolić, Miodrag, "Mesenchymal stem cells from periapical lesions modulate cytokine production by local immune cells" in Vojnosanitetski pregled, 75, no. 5 (2018):473-480,
https://doi.org/10.2298/VSP160901272M . .

TY  - JOUR
AU  - Marković, M.
AU  - Tomić, S.
AU  - Đokić, Jelena
AU  - Čolić, Miodrag
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/860
AB  - The pathophysiology of periapical lesions (PLs) is under control of pro-inflammatory and anti-inflammatory (mainly immunoregulatory) cytokines. We have recently established mesenchymal stem cells (MSCs) from PLs and showed their suppressive effects on the production of proinflammatory cytokines from PLs inflammatory cells (ICs). In this work we studied the production of interleukin (IL)-10, IL-27 and transforming growth factor (TGF)-β, by PL-ICs in direct or indirect contacts with PL-MSCs. PL-ICs, which were isolated from four different asymptomatic PLs, predominantly composed of lymphocytes, followed by neutrophil granulocytes, macrophages and plasma cells. PLMSCs, expressing typical MSC markers, were co-cultivated with PL-ICs at 1:10 ratio, either in direct contact or in a transwell-system, for 24 hours. The levels of cytokines in cell-culture supernatants were tested by ELISA. The results showed that PL-MSCs up-regulated the production of all three immunoregulatory cytokines by PL-ICs. PL-MSCs stimulated the production of IL-10 and IL-27 via soluble factors, whereas the up-regulation of TGF-β required direct cell-to-cell contacts. In conclusion, our results showed for the first time the involvement of PL-MSCs in restriction of inflammation in PLs by up-regulation of immunoregulatory cytokines.
PB  - Univerzitet u Nišu - Medicinski fakultet, Niš
T2  - Acta Facultatis Medicae Naissensis
T1  - Mesenchymal Stem Cells from Periapical Lesions Upregulate the Production of Immunoregulatory Cytokines by Inflammatory Cells in Culture
EP  - 179
IS  - 3
SP  - 171
VL  - 32
DO  - 10.1515/afmnai-2015-0017
ER  - 

@article{
author = "Marković, M. and Tomić, S. and Đokić, Jelena and Čolić, Miodrag",
year = "2015",
abstract = "The pathophysiology of periapical lesions (PLs) is under control of pro-inflammatory and anti-inflammatory (mainly immunoregulatory) cytokines. We have recently established mesenchymal stem cells (MSCs) from PLs and showed their suppressive effects on the production of proinflammatory cytokines from PLs inflammatory cells (ICs). In this work we studied the production of interleukin (IL)-10, IL-27 and transforming growth factor (TGF)-β, by PL-ICs in direct or indirect contacts with PL-MSCs. PL-ICs, which were isolated from four different asymptomatic PLs, predominantly composed of lymphocytes, followed by neutrophil granulocytes, macrophages and plasma cells. PLMSCs, expressing typical MSC markers, were co-cultivated with PL-ICs at 1:10 ratio, either in direct contact or in a transwell-system, for 24 hours. The levels of cytokines in cell-culture supernatants were tested by ELISA. The results showed that PL-MSCs up-regulated the production of all three immunoregulatory cytokines by PL-ICs. PL-MSCs stimulated the production of IL-10 and IL-27 via soluble factors, whereas the up-regulation of TGF-β required direct cell-to-cell contacts. In conclusion, our results showed for the first time the involvement of PL-MSCs in restriction of inflammation in PLs by up-regulation of immunoregulatory cytokines.",
publisher = "Univerzitet u Nišu - Medicinski fakultet, Niš",
journal = "Acta Facultatis Medicae Naissensis",
title = "Mesenchymal Stem Cells from Periapical Lesions Upregulate the Production of Immunoregulatory Cytokines by Inflammatory Cells in Culture",
pages = "179-171",
number = "3",
volume = "32",
doi = "10.1515/afmnai-2015-0017"
}

Marković, M., Tomić, S., Đokić, J.,& Čolić, M.. (2015). Mesenchymal Stem Cells from Periapical Lesions Upregulate the Production of Immunoregulatory Cytokines by Inflammatory Cells in Culture. in Acta Facultatis Medicae Naissensis
Univerzitet u Nišu - Medicinski fakultet, Niš., 32(3), 171-179.
https://doi.org/10.1515/afmnai-2015-0017

Marković M, Tomić S, Đokić J, Čolić M. Mesenchymal Stem Cells from Periapical Lesions Upregulate the Production of Immunoregulatory Cytokines by Inflammatory Cells in Culture. in Acta Facultatis Medicae Naissensis. 2015;32(3):171-179.
doi:10.1515/afmnai-2015-0017 .

Marković, M., Tomić, S., Đokić, Jelena, Čolić, Miodrag, "Mesenchymal Stem Cells from Periapical Lesions Upregulate the Production of Immunoregulatory Cytokines by Inflammatory Cells in Culture" in Acta Facultatis Medicae Naissensis, 32, no. 3 (2015):171-179,
https://doi.org/10.1515/afmnai-2015-0017 . .

TY  - JOUR
AU  - Pavlović, Bojan
AU  - Tomić, Sergej
AU  - Đokić, Jelena
AU  - Vasilijić, Sasa
AU  - Vucević, Dragana
AU  - Lukić, Jovanka
AU  - Gruden-Movsesijan, Alisa
AU  - Ilić, Nataša
AU  - Marković, Milan
AU  - Čolić, Miodrag
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/846
AB  - Background aims. Because of the labor-intensive and time-consuming conventional protocols for the generation of dendritic cells (DCs) as the most promising tools for anti-cancer therapy that enable the induction of a T-helper (Th)1-mediated anti-tumor immune response, the use of short-term protocols has been proposed. However, data on the applicability of such protocols in cancer immunotherapy are quite limited. Methods. We compared the phenotypic and functional capability of fast DCs (fDCs) differentiated for 24 h and then matured for 48 h with Poly (I:C), a strong Th1-promoting agent, with donor-matched conventional DCs (cDCs) differentiated for 5 days and matured likewise. Results. Of 12 donors tested, we identified seven whose monocytes failed to develop into immunogenic DCs through the use of fDC protocol, on the basis of incomplete downregulation of CD 14, low expression of CD la and macrophage-like morphology. Such fDCs have significantly lower expression of CD83, CD86, CCR7 and CD40, weaker allo-stimulatory Th1- and Th17-polarizing capacity caused by poor production of interleukin (IL)-12p70 and IL-23 and high production of IL-10, and prominent Th2-polarizing capacity, compared with donor-matched cDCs. Furthermore, such fDCs had tolerogenic properties as judged by higher expression of indolamine dioxigenase-3, IDO-1 and IL-1 beta and induction of a higher percentage of CD4(+)CD25(+)FoxP3(+) T cells. These findings correlated with increased transforming growth factor (TGF)-beta production by fDC-primed CD3(+)T cells and their stronger antiproliferative capacity. Conclusions. We emphasize that although fDCs could probably be applied as an alternative to cDCs for cancer therapy, the fDC protocol should not be applied to donors whose DCs acquire tolerogenic capabilities.
PB  - Elsevier Sci Ltd, Oxford
T2  - Cytotherapy
T1  - Fast dendritic cells matured with Poly (I:C) may acquire tolerogenic properties
EP  - 1776
IS  - 12
SP  - 1763
VL  - 17
DO  - 10.1016/j.jcyt.2015.08.001
ER  - 

@article{
author = "Pavlović, Bojan and Tomić, Sergej and Đokić, Jelena and Vasilijić, Sasa and Vucević, Dragana and Lukić, Jovanka and Gruden-Movsesijan, Alisa and Ilić, Nataša and Marković, Milan and Čolić, Miodrag",
year = "2015",
abstract = "Background aims. Because of the labor-intensive and time-consuming conventional protocols for the generation of dendritic cells (DCs) as the most promising tools for anti-cancer therapy that enable the induction of a T-helper (Th)1-mediated anti-tumor immune response, the use of short-term protocols has been proposed. However, data on the applicability of such protocols in cancer immunotherapy are quite limited. Methods. We compared the phenotypic and functional capability of fast DCs (fDCs) differentiated for 24 h and then matured for 48 h with Poly (I:C), a strong Th1-promoting agent, with donor-matched conventional DCs (cDCs) differentiated for 5 days and matured likewise. Results. Of 12 donors tested, we identified seven whose monocytes failed to develop into immunogenic DCs through the use of fDC protocol, on the basis of incomplete downregulation of CD 14, low expression of CD la and macrophage-like morphology. Such fDCs have significantly lower expression of CD83, CD86, CCR7 and CD40, weaker allo-stimulatory Th1- and Th17-polarizing capacity caused by poor production of interleukin (IL)-12p70 and IL-23 and high production of IL-10, and prominent Th2-polarizing capacity, compared with donor-matched cDCs. Furthermore, such fDCs had tolerogenic properties as judged by higher expression of indolamine dioxigenase-3, IDO-1 and IL-1 beta and induction of a higher percentage of CD4(+)CD25(+)FoxP3(+) T cells. These findings correlated with increased transforming growth factor (TGF)-beta production by fDC-primed CD3(+)T cells and their stronger antiproliferative capacity. Conclusions. We emphasize that although fDCs could probably be applied as an alternative to cDCs for cancer therapy, the fDC protocol should not be applied to donors whose DCs acquire tolerogenic capabilities.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Cytotherapy",
title = "Fast dendritic cells matured with Poly (I:C) may acquire tolerogenic properties",
pages = "1776-1763",
number = "12",
volume = "17",
doi = "10.1016/j.jcyt.2015.08.001"
}

Pavlović, B., Tomić, S., Đokić, J., Vasilijić, S., Vucević, D., Lukić, J., Gruden-Movsesijan, A., Ilić, N., Marković, M.,& Čolić, M.. (2015). Fast dendritic cells matured with Poly (I:C) may acquire tolerogenic properties. in Cytotherapy
Elsevier Sci Ltd, Oxford., 17(12), 1763-1776.
https://doi.org/10.1016/j.jcyt.2015.08.001

Pavlović B, Tomić S, Đokić J, Vasilijić S, Vucević D, Lukić J, Gruden-Movsesijan A, Ilić N, Marković M, Čolić M. Fast dendritic cells matured with Poly (I:C) may acquire tolerogenic properties. in Cytotherapy. 2015;17(12):1763-1776.
doi:10.1016/j.jcyt.2015.08.001 .

Pavlović, Bojan, Tomić, Sergej, Đokić, Jelena, Vasilijić, Sasa, Vucević, Dragana, Lukić, Jovanka, Gruden-Movsesijan, Alisa, Ilić, Nataša, Marković, Milan, Čolić, Miodrag, "Fast dendritic cells matured with Poly (I:C) may acquire tolerogenic properties" in Cytotherapy, 17, no. 12 (2015):1763-1776,
https://doi.org/10.1016/j.jcyt.2015.08.001 . .

TY  - THES
AU  - Đokić, Jelena
PY  - 2014
UR  - https://nardus.mpn.gov.rs/handle/123456789/6333
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=3699
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:12515/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024765618
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/33
AB  - Mezenhimske matične ćelije (engl. mesenchymal stem cells, MSC) izolovane iz različitih tkiva se sve više ispituju kao potencijalno sredstvo u terapiji oštećenja tkiva i/ili inflamacijskih bolesti. U poređenju sa MSC iz kostne srži, MSC iz dentalnih tkiva su znatno pristupačnije. Međutim, potencijal za primenu MSC iz dentalnih tkiva u ćelijskoj terapiji je još nedovoljno ispitan, obzirom na to da se MSC iz različitih dentalnih tkiva razlikuju po potencijalu za diferencijaciju, i da podaci o njihovim imunomodulatornim osobinama gotovo da ne postoje. Stoga je cilj ove teze bio da se uporede fenotipske osobine, sposobnost diferencijacije i imunomodulatorne osobine MSC iz različitih dentalnih tkiva zdravih donora. Takođe, nije poznato da li faktori inflamacije i infekcije mogu modifikovati imunomodulatorne osobine ovih MSC, i koji mehanizmi su uključeni u ove procese. U tom smislu je ispitivano da li su imunomodulatorne osobine MSC izolovanih iz zdravih dentalnih tkiva podložne modulaciji agonistima Toll-u sličnih receptora (TLR), važnim inicijatorima imunskog odgovora u tkivu. Pored toga, jedan od ciljeva je bio ispitati da li su fenotip, sposobnost za diferencijaciju i imunomodulatorne osobine MSC izolovanih iz hronične inflamacije, kakva je periapeksna lezija (PL), izmenjene u odnosu na MSC iz zdravih dentalnih tkiva. Takođe, nije poznato kakve efekte PL-MSC ostvaruju na inflamacijske ćelije periapeksne lezije, i koji su potencijalni mehanizmi odgovorni za njihov efekat. U tom smislu bi proučavanje interakcije PL-MSC sa dendritskih ćelijama (DC), ključnim inicijatorima i regulatorima imunskog odgovora, značajno doprinelo razumevanju imunoregulatornih mehanizama MSC u inflamaciji, zbog čega je ispitivanje ovih interakcija bio važan cilj naših istraživanja. U svrhu ovih ispitivanja humane MSC su izolovane kao adherentne ćelije nakon kolagenazno/DNKznog tretmana dentalne pulpe (DP) i dentalnog folikula (DF) zdravog trećeg molara, i periapeksne lezije...
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Karakterizacija i ispitivanje imunomodulatornih svojstava mezenhimskih matičnih ćelija izolovanih iz dentalnog tkiva čoveka
T1  - Characterisation and analysis of immunomodulatory properties of mesenchymal stem cells isolated from human dental tissues
UR  - https://hdl.handle.net/21.15107/rcub_nardus_6333
ER  - 

@phdthesis{
author = "Đokić, Jelena",
year = "2014",
abstract = "Mezenhimske matične ćelije (engl. mesenchymal stem cells, MSC) izolovane iz različitih tkiva se sve više ispituju kao potencijalno sredstvo u terapiji oštećenja tkiva i/ili inflamacijskih bolesti. U poređenju sa MSC iz kostne srži, MSC iz dentalnih tkiva su znatno pristupačnije. Međutim, potencijal za primenu MSC iz dentalnih tkiva u ćelijskoj terapiji je još nedovoljno ispitan, obzirom na to da se MSC iz različitih dentalnih tkiva razlikuju po potencijalu za diferencijaciju, i da podaci o njihovim imunomodulatornim osobinama gotovo da ne postoje. Stoga je cilj ove teze bio da se uporede fenotipske osobine, sposobnost diferencijacije i imunomodulatorne osobine MSC iz različitih dentalnih tkiva zdravih donora. Takođe, nije poznato da li faktori inflamacije i infekcije mogu modifikovati imunomodulatorne osobine ovih MSC, i koji mehanizmi su uključeni u ove procese. U tom smislu je ispitivano da li su imunomodulatorne osobine MSC izolovanih iz zdravih dentalnih tkiva podložne modulaciji agonistima Toll-u sličnih receptora (TLR), važnim inicijatorima imunskog odgovora u tkivu. Pored toga, jedan od ciljeva je bio ispitati da li su fenotip, sposobnost za diferencijaciju i imunomodulatorne osobine MSC izolovanih iz hronične inflamacije, kakva je periapeksna lezija (PL), izmenjene u odnosu na MSC iz zdravih dentalnih tkiva. Takođe, nije poznato kakve efekte PL-MSC ostvaruju na inflamacijske ćelije periapeksne lezije, i koji su potencijalni mehanizmi odgovorni za njihov efekat. U tom smislu bi proučavanje interakcije PL-MSC sa dendritskih ćelijama (DC), ključnim inicijatorima i regulatorima imunskog odgovora, značajno doprinelo razumevanju imunoregulatornih mehanizama MSC u inflamaciji, zbog čega je ispitivanje ovih interakcija bio važan cilj naših istraživanja. U svrhu ovih ispitivanja humane MSC su izolovane kao adherentne ćelije nakon kolagenazno/DNKznog tretmana dentalne pulpe (DP) i dentalnog folikula (DF) zdravog trećeg molara, i periapeksne lezije...",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Karakterizacija i ispitivanje imunomodulatornih svojstava mezenhimskih matičnih ćelija izolovanih iz dentalnog tkiva čoveka, Characterisation and analysis of immunomodulatory properties of mesenchymal stem cells isolated from human dental tissues",
url = "https://hdl.handle.net/21.15107/rcub_nardus_6333"
}

Đokić, J.. (2014). Karakterizacija i ispitivanje imunomodulatornih svojstava mezenhimskih matičnih ćelija izolovanih iz dentalnog tkiva čoveka. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_6333

Đokić J. Karakterizacija i ispitivanje imunomodulatornih svojstava mezenhimskih matičnih ćelija izolovanih iz dentalnog tkiva čoveka. 2014;.
https://hdl.handle.net/21.15107/rcub_nardus_6333 .

Đokić, Jelena, "Karakterizacija i ispitivanje imunomodulatornih svojstava mezenhimskih matičnih ćelija izolovanih iz dentalnog tkiva čoveka" (2014),
https://hdl.handle.net/21.15107/rcub_nardus_6333 .

TY  - JOUR
AU  - Đokić, Jelena
AU  - Tomić, Sergej
AU  - Marković, Milan
AU  - Milosavljević, Petar
AU  - Čolić, Miodrag
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/701
AB  - Immunoregulatory mechanisms within periapical lesions (PLs) are as of yet unexplored. Considering the crucial role of DCs in controlling the immune response within PLs, the immunomodulatory properties of mesenchymal stem cells (MSCs), and the colocalization of MSCs and DCs in situ, we wondered whether MSCs from PLs modulate the development and functions of DCs. Using a model of monocyte-derived DCs, we showed that PL-MSCs inhibited differentiation of DCs via soluble factors, of which IL-6 had a minor effect, but did not impair their subsequent maturation induced by pro-inflammatory cytokines. However, upon maturation such DCs favored the production of Th2/Th17 cytokines by allogenic CD4(+) lymphocytes in coculture, compared with mature DCs differentiated without PL-MSCs. PL-MSC-differentiated DCs, cultivated with pro-inflammatory cytokines and PL-MSCs, although phenotypically mature, exhibited poor allostimulatory activity, induced anergy, Th2 polarization, differentiation of suppressive CD4(+)CD25(high)CD39(+) Treg-cell subsets via IDO-1-, ILT-3-, and ILT-4-dependent mechanisms, and increased production of TGF- in the coculture. In contrast, DCs cultivated with PL-MSCs only during maturation stimulated proliferation and Th1 polarization of CD4(+) T cells in an IL-12-independent manner. In conclusion, PL-MSCs significantly modulate the development and functions of DCs, depending on the phase of DCs development during which the interaction occurs.
PB  - Wiley-Blackwell, Hoboken
T2  - European Journal of Immunology
T1  - Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells
EP  - 1872
IS  - 7
SP  - 1862
VL  - 43
DO  - 10.1002/eji.201243010
ER  - 

@article{
author = "Đokić, Jelena and Tomić, Sergej and Marković, Milan and Milosavljević, Petar and Čolić, Miodrag",
year = "2013",
abstract = "Immunoregulatory mechanisms within periapical lesions (PLs) are as of yet unexplored. Considering the crucial role of DCs in controlling the immune response within PLs, the immunomodulatory properties of mesenchymal stem cells (MSCs), and the colocalization of MSCs and DCs in situ, we wondered whether MSCs from PLs modulate the development and functions of DCs. Using a model of monocyte-derived DCs, we showed that PL-MSCs inhibited differentiation of DCs via soluble factors, of which IL-6 had a minor effect, but did not impair their subsequent maturation induced by pro-inflammatory cytokines. However, upon maturation such DCs favored the production of Th2/Th17 cytokines by allogenic CD4(+) lymphocytes in coculture, compared with mature DCs differentiated without PL-MSCs. PL-MSC-differentiated DCs, cultivated with pro-inflammatory cytokines and PL-MSCs, although phenotypically mature, exhibited poor allostimulatory activity, induced anergy, Th2 polarization, differentiation of suppressive CD4(+)CD25(high)CD39(+) Treg-cell subsets via IDO-1-, ILT-3-, and ILT-4-dependent mechanisms, and increased production of TGF- in the coculture. In contrast, DCs cultivated with PL-MSCs only during maturation stimulated proliferation and Th1 polarization of CD4(+) T cells in an IL-12-independent manner. In conclusion, PL-MSCs significantly modulate the development and functions of DCs, depending on the phase of DCs development during which the interaction occurs.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "European Journal of Immunology",
title = "Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells",
pages = "1872-1862",
number = "7",
volume = "43",
doi = "10.1002/eji.201243010"
}

Đokić, J., Tomić, S., Marković, M., Milosavljević, P.,& Čolić, M.. (2013). Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells. in European Journal of Immunology
Wiley-Blackwell, Hoboken., 43(7), 1862-1872.
https://doi.org/10.1002/eji.201243010

Đokić J, Tomić S, Marković M, Milosavljević P, Čolić M. Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells. in European Journal of Immunology. 2013;43(7):1862-1872.
doi:10.1002/eji.201243010 .

Đokić, Jelena, Tomić, Sergej, Marković, Milan, Milosavljević, Petar, Čolić, Miodrag, "Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells" in European Journal of Immunology, 43, no. 7 (2013):1862-1872,
https://doi.org/10.1002/eji.201243010 . .

TY  - JOUR
AU  - Đokić, Jelena
AU  - Rudolf, Rebeka
AU  - Tomić, Sergej
AU  - Stopić, Srecko
AU  - Friedrich, Bernd
AU  - Budić, Bojan
AU  - Anzel, Ivan
AU  - Čolić, Miodrag
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/559
AB  - We prepared 5 different fractions of nanoparticles from the gold scrap, by using a new technology, Ultrasonic Spray Pirolysis (USP). The aim of this study was to characterize the microstructure and cytotoxicity of the nanoparticles along with their immunomodulatory properties, using Concanavaline A (ConA)-treated rat splenocytes as a model of activated immune cells. Fractions 1 and 2, composed of pure gold nanoparticles, although non-cytotoxic, reduced cellular proliferation. Fraction 2, containing particles smaller in size and lesser agglomerated than fraction 1, up- and down-regulated the production of IL-2 and IL-10, respectively, by activated splenocytes. Fraction 3, containing nanoparticles composed of Au and up to 3 at.% Cu, was non-cytotoxic, but reduced IL-2 production and cell proliferation. Fractions 4 and 5, contaminated with alloying elements from the gold scrap, were cytotoxic. The extent of cytotoxicity and subsequent reduction of cytokine production, as well as the mode of cell death, depended on their composition. In conclusion, we showed that USP enables the synthesis of gold nanoparticles, which could be suitable for various biological applications, and that ConA-treated splenocytes represent a reliable model for fast and accurate evaluation of the immunotoxicological profiles of these particles. However, it is necessary to improve this technology and investigate further some of the immunomodulatory mechanisms using more specific immunological tests.
PB  - Amer Scientific Publishers, Valencia
T2  - Journal of Biomedical Nanotechnology
T1  - Immunomodulatory Properties of Nanoparticles Obtained by Ultrasonic Spray Pirolysis from Gold Scrap
EP  - 538
IS  - 3
SP  - 528
VL  - 8
DO  - 10.1166/jbn.2012.1405
ER  - 

@article{
author = "Đokić, Jelena and Rudolf, Rebeka and Tomić, Sergej and Stopić, Srecko and Friedrich, Bernd and Budić, Bojan and Anzel, Ivan and Čolić, Miodrag",
year = "2012",
abstract = "We prepared 5 different fractions of nanoparticles from the gold scrap, by using a new technology, Ultrasonic Spray Pirolysis (USP). The aim of this study was to characterize the microstructure and cytotoxicity of the nanoparticles along with their immunomodulatory properties, using Concanavaline A (ConA)-treated rat splenocytes as a model of activated immune cells. Fractions 1 and 2, composed of pure gold nanoparticles, although non-cytotoxic, reduced cellular proliferation. Fraction 2, containing particles smaller in size and lesser agglomerated than fraction 1, up- and down-regulated the production of IL-2 and IL-10, respectively, by activated splenocytes. Fraction 3, containing nanoparticles composed of Au and up to 3 at.% Cu, was non-cytotoxic, but reduced IL-2 production and cell proliferation. Fractions 4 and 5, contaminated with alloying elements from the gold scrap, were cytotoxic. The extent of cytotoxicity and subsequent reduction of cytokine production, as well as the mode of cell death, depended on their composition. In conclusion, we showed that USP enables the synthesis of gold nanoparticles, which could be suitable for various biological applications, and that ConA-treated splenocytes represent a reliable model for fast and accurate evaluation of the immunotoxicological profiles of these particles. However, it is necessary to improve this technology and investigate further some of the immunomodulatory mechanisms using more specific immunological tests.",
publisher = "Amer Scientific Publishers, Valencia",
journal = "Journal of Biomedical Nanotechnology",
title = "Immunomodulatory Properties of Nanoparticles Obtained by Ultrasonic Spray Pirolysis from Gold Scrap",
pages = "538-528",
number = "3",
volume = "8",
doi = "10.1166/jbn.2012.1405"
}

Đokić, J., Rudolf, R., Tomić, S., Stopić, S., Friedrich, B., Budić, B., Anzel, I.,& Čolić, M.. (2012). Immunomodulatory Properties of Nanoparticles Obtained by Ultrasonic Spray Pirolysis from Gold Scrap. in Journal of Biomedical Nanotechnology
Amer Scientific Publishers, Valencia., 8(3), 528-538.
https://doi.org/10.1166/jbn.2012.1405

Đokić J, Rudolf R, Tomić S, Stopić S, Friedrich B, Budić B, Anzel I, Čolić M. Immunomodulatory Properties of Nanoparticles Obtained by Ultrasonic Spray Pirolysis from Gold Scrap. in Journal of Biomedical Nanotechnology. 2012;8(3):528-538.
doi:10.1166/jbn.2012.1405 .

Đokić, Jelena, Rudolf, Rebeka, Tomić, Sergej, Stopić, Srecko, Friedrich, Bernd, Budić, Bojan, Anzel, Ivan, Čolić, Miodrag, "Immunomodulatory Properties of Nanoparticles Obtained by Ultrasonic Spray Pirolysis from Gold Scrap" in Journal of Biomedical Nanotechnology, 8, no. 3 (2012):528-538,
https://doi.org/10.1166/jbn.2012.1405 . .