TY  - CONF
AU  - Drakulić, Danijela
AU  - Cuturilo, Goran
AU  - Jovanović, Ida
AU  - Krstić, Aleksandar
AU  - Milivojević, Milena
AU  - Stevanović, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2181
AB  - Background/Objectives: 22q11.2 microdeletion, detected in
patients with 22q11.2 Deletion Syndrome (22q11.2DS), is the most
common microdeletion syndrome in humans. 22q11.2DS has high
risk for neurodevelopmental disorders and is associated with more than 180 malformations. Many investigations of the 22q11.2
microdeletion applying different recruitment criteria, revealed
detection rate ranging from zero to 34.7%. Here we analyzed the
frequency of 22q11.2 microdeletion among children having at
least two out of five major characteristics of 22q11.2DS: congenital
heart malformations (CHM), facial dysmorphism, immunological
problems, palatal clefts and hypocalcemia.
Methods: Children with clinical characteristics of 22q11.2DS
were analyzed. Fluorescence in situ hybridization and multiplex
ligation-dependent probe amplification analysis were applied for
detection of 22q11.2 microdeletion.
Results: 22q11.2 microdeletion was detected in approximately
40% of children. CHM was found in all patients with 22q11.2
microdeletion. Dysmorphic facial features were present in about
45%, immunological problems in 30%, overt cleft palate in about
4% and hypocalcemia in approximately 60% of patients with
22q11.2 microdeletion.
Conclusion: When at least two major features of 22q11.2DS are
taking into consideration higher detection rate is obtained compared
to one-feature criterion. These criteria could be considered
by centers in low-income countries.
PB  - Springer Nature
C3  - European Journal of Human Genetics
T1  - Detection rate of 22q11.2 microdeletion using strict diagnostic criteria
EP  - 240
IS  - Suppl 1
SP  - 240
VL  - 31
DO  - 10.1038/s41431-023-01339-3
ER  - 

@conference{
author = "Drakulić, Danijela and Cuturilo, Goran and Jovanović, Ida and Krstić, Aleksandar and Milivojević, Milena and Stevanović, Milena",
year = "2023",
abstract = "Background/Objectives: 22q11.2 microdeletion, detected in
patients with 22q11.2 Deletion Syndrome (22q11.2DS), is the most
common microdeletion syndrome in humans. 22q11.2DS has high
risk for neurodevelopmental disorders and is associated with more than 180 malformations. Many investigations of the 22q11.2
microdeletion applying different recruitment criteria, revealed
detection rate ranging from zero to 34.7%. Here we analyzed the
frequency of 22q11.2 microdeletion among children having at
least two out of five major characteristics of 22q11.2DS: congenital
heart malformations (CHM), facial dysmorphism, immunological
problems, palatal clefts and hypocalcemia.
Methods: Children with clinical characteristics of 22q11.2DS
were analyzed. Fluorescence in situ hybridization and multiplex
ligation-dependent probe amplification analysis were applied for
detection of 22q11.2 microdeletion.
Results: 22q11.2 microdeletion was detected in approximately
40% of children. CHM was found in all patients with 22q11.2
microdeletion. Dysmorphic facial features were present in about
45%, immunological problems in 30%, overt cleft palate in about
4% and hypocalcemia in approximately 60% of patients with
22q11.2 microdeletion.
Conclusion: When at least two major features of 22q11.2DS are
taking into consideration higher detection rate is obtained compared
to one-feature criterion. These criteria could be considered
by centers in low-income countries.",
publisher = "Springer Nature",
journal = "European Journal of Human Genetics",
title = "Detection rate of 22q11.2 microdeletion using strict diagnostic criteria",
pages = "240-240",
number = "Suppl 1",
volume = "31",
doi = "10.1038/s41431-023-01339-3"
}

Drakulić, D., Cuturilo, G., Jovanović, I., Krstić, A., Milivojević, M.,& Stevanović, M.. (2023). Detection rate of 22q11.2 microdeletion using strict diagnostic criteria. in European Journal of Human Genetics
Springer Nature., 31(Suppl 1), 240-240.
https://doi.org/10.1038/s41431-023-01339-3

Drakulić D, Cuturilo G, Jovanović I, Krstić A, Milivojević M, Stevanović M. Detection rate of 22q11.2 microdeletion using strict diagnostic criteria. in European Journal of Human Genetics. 2023;31(Suppl 1):240-240.
doi:10.1038/s41431-023-01339-3 .

Drakulić, Danijela, Cuturilo, Goran, Jovanović, Ida, Krstić, Aleksandar, Milivojević, Milena, Stevanović, Milena, "Detection rate of 22q11.2 microdeletion using strict diagnostic criteria" in European Journal of Human Genetics, 31, no. Suppl 1 (2023):240-240,
https://doi.org/10.1038/s41431-023-01339-3 . .

TY  - CONF
AU  - Marjanović, Jelena
AU  - Drakulić, Danijela
AU  - Garcia, Idoia
AU  - Vuković, Vladanka
AU  - Aldaz, Paula
AU  - Garros-Regulez, Laura
AU  - Sampron, Nicolas
AU  - Matheu, Ander
AU  - Stevanović, Milena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1550
PB  - Springernature, London
C3  - European Journal of Human Genetics
T1  - SOX3 function in glioblastoma cells
EP  - 429
IS  - SUPPL 1
SP  - 428
VL  - 30
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1550
ER  - 

@conference{
author = "Marjanović, Jelena and Drakulić, Danijela and Garcia, Idoia and Vuković, Vladanka and Aldaz, Paula and Garros-Regulez, Laura and Sampron, Nicolas and Matheu, Ander and Stevanović, Milena",
year = "2022",
publisher = "Springernature, London",
journal = "European Journal of Human Genetics",
title = "SOX3 function in glioblastoma cells",
pages = "429-428",
number = "SUPPL 1",
volume = "30",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1550"
}

Marjanović, J., Drakulić, D., Garcia, I., Vuković, V., Aldaz, P., Garros-Regulez, L., Sampron, N., Matheu, A.,& Stevanović, M.. (2022). SOX3 function in glioblastoma cells. in European Journal of Human Genetics
Springernature, London., 30(SUPPL 1), 428-429.
https://hdl.handle.net/21.15107/rcub_imagine_1550

Marjanović J, Drakulić D, Garcia I, Vuković V, Aldaz P, Garros-Regulez L, Sampron N, Matheu A, Stevanović M. SOX3 function in glioblastoma cells. in European Journal of Human Genetics. 2022;30(SUPPL 1):428-429.
https://hdl.handle.net/21.15107/rcub_imagine_1550 .

Marjanović, Jelena, Drakulić, Danijela, Garcia, Idoia, Vuković, Vladanka, Aldaz, Paula, Garros-Regulez, Laura, Sampron, Nicolas, Matheu, Ander, Stevanović, Milena, "SOX3 function in glioblastoma cells" in European Journal of Human Genetics, 30, no. SUPPL 1 (2022):428-429,
https://hdl.handle.net/21.15107/rcub_imagine_1550 .

TY  - JOUR
AU  - Lazić, Andrijana
AU  - Popović, Jelena
AU  - Paunesku, Tatjana
AU  - Woloschak, Gayle E.
AU  - Stevanović, Milena
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1385
AB  - Cancer is a global health problem that is often successfully addressed by therapy, with cancer survivors increasing in numbers and living longer world around. Although new cancer treatment options are continuously explored, platinum based chemotherapy agents remain in use due to their efficiency and availability. Unfortunately, all cancer therapies affect normal tissues as well as cancer, and more than 40 specific side effects of platinum based drugs documented so far decrease the quality of life of cancer survivors. Chemotherapy-induced peripheral neuropathy is a frequent side effects of platinum-based chemotherapy agents. This cluster of complications is often so debilitating that patients occasionally have to discontinue the therapy. Sensory neurons of dorsal root ganglia are at the core of chemotherapy-induced peripheral neuropathy symptoms. In these postmitotic cells, DNA damage caused by platinum chemotherapy interferes with normal functioning. Accumulation of DNA-platinum adducts correlates with neurotoxic severity and development of sensation of pain. While biochemistry of DNA-platinum adducts is the same in all cell types, molecular mechanisms affected by DNA-platinum adducts are different in cancer cells and non-dividing cells. This review aims to raise awareness about platinum associated chemotherapy-induced peripheral neuropathy as a medical problem that has remained unexplained for decades. We emphasize the complexity of this condition both from clinical and mechanistical point of view and focus on recent findings about chemotherapy-induced peripheral neuropathy in in vitro and in vivo model systems. Finally, we summarize current perspectives about clinical approaches for chemotherapy-induced peripheral neuropathy treatment.
PB  - Wolters Kluwer Medknow Publications, Mumbai
T2  - Neural Regeneration Research
T1  - Insights into platinum-induced peripheral neuropathy-current perspective
EP  - 1630
IS  - 9
SP  - 1623
VL  - 15
DO  - 10.4103/1673-5374.276321
ER  - 

@article{
author = "Lazić, Andrijana and Popović, Jelena and Paunesku, Tatjana and Woloschak, Gayle E. and Stevanović, Milena",
year = "2020",
abstract = "Cancer is a global health problem that is often successfully addressed by therapy, with cancer survivors increasing in numbers and living longer world around. Although new cancer treatment options are continuously explored, platinum based chemotherapy agents remain in use due to their efficiency and availability. Unfortunately, all cancer therapies affect normal tissues as well as cancer, and more than 40 specific side effects of platinum based drugs documented so far decrease the quality of life of cancer survivors. Chemotherapy-induced peripheral neuropathy is a frequent side effects of platinum-based chemotherapy agents. This cluster of complications is often so debilitating that patients occasionally have to discontinue the therapy. Sensory neurons of dorsal root ganglia are at the core of chemotherapy-induced peripheral neuropathy symptoms. In these postmitotic cells, DNA damage caused by platinum chemotherapy interferes with normal functioning. Accumulation of DNA-platinum adducts correlates with neurotoxic severity and development of sensation of pain. While biochemistry of DNA-platinum adducts is the same in all cell types, molecular mechanisms affected by DNA-platinum adducts are different in cancer cells and non-dividing cells. This review aims to raise awareness about platinum associated chemotherapy-induced peripheral neuropathy as a medical problem that has remained unexplained for decades. We emphasize the complexity of this condition both from clinical and mechanistical point of view and focus on recent findings about chemotherapy-induced peripheral neuropathy in in vitro and in vivo model systems. Finally, we summarize current perspectives about clinical approaches for chemotherapy-induced peripheral neuropathy treatment.",
publisher = "Wolters Kluwer Medknow Publications, Mumbai",
journal = "Neural Regeneration Research",
title = "Insights into platinum-induced peripheral neuropathy-current perspective",
pages = "1630-1623",
number = "9",
volume = "15",
doi = "10.4103/1673-5374.276321"
}

Lazić, A., Popović, J., Paunesku, T., Woloschak, G. E.,& Stevanović, M.. (2020). Insights into platinum-induced peripheral neuropathy-current perspective. in Neural Regeneration Research
Wolters Kluwer Medknow Publications, Mumbai., 15(9), 1623-1630.
https://doi.org/10.4103/1673-5374.276321

Lazić A, Popović J, Paunesku T, Woloschak GE, Stevanović M. Insights into platinum-induced peripheral neuropathy-current perspective. in Neural Regeneration Research. 2020;15(9):1623-1630.
doi:10.4103/1673-5374.276321 .

Lazić, Andrijana, Popović, Jelena, Paunesku, Tatjana, Woloschak, Gayle E., Stevanović, Milena, "Insights into platinum-induced peripheral neuropathy-current perspective" in Neural Regeneration Research, 15, no. 9 (2020):1623-1630,
https://doi.org/10.4103/1673-5374.276321 . .

TY  - JOUR
AU  - Drakulić, Danijela
AU  - Đurović, Srđan
AU  - Syed, Yasir Ahmed
AU  - Trattaro, Sebastiano
AU  - Caporale, Nicolo
AU  - Falk, Anna
AU  - Ofir, Rivka
AU  - Heine, Vivi M.
AU  - Chawner, Samuel J. R. A.
AU  - Rodriguez-Moreno, Antonio
AU  - van den Bree, Marianne B. M.
AU  - Testa, Giuseppe
AU  - Petrakis, Spyros
AU  - Harwood, Adrian J.
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1330
AB  - Patients diagnosed with chromosome microdeletions or duplications, known as copy number variants (CNVs), present a unique opportunity to investigate the relationship between patient genotype and cell phenotype. CNVs have high genetic penetrance and give a good correlation between gene locus and patient clinical phenotype. This is especially effective for the study of patients with neurodevelopmental disorders (NDD), including those falling within the autism spectrum disorders (ASD). A key question is whether this correlation between genetics and clinical presentation at the level of the patient can be translated to the cell phenotypes arising from the neurodevelopment of patient induced pluripotent stem cells (iPSCs). Here, we examine how iPSCs derived from ASD patients with an associated CNV inform our understanding of the genetic and biological mechanisms underlying the aetiology of ASD. We consider selection of genetically characterised patient iPSCs; use of appropriate control lines; aspects of human neurocellular biology that can capture in vitro the patient clinical phenotype; and current limitations of patient iPSC-based studies. Finally, we consider how future research may be enhanced to maximise the utility of CNV patients for research of pathological mechanisms or therapeutic targets.
PB  - BMC, London
T2  - Molecular Autism
T1  - Copy number variants (CNVs): a powerful tool for iPSC-based modelling of ASD
IS  - 1
VL  - 11
DO  - 10.1186/s13229-020-00343-4
ER  - 

@article{
author = "Drakulić, Danijela and Đurović, Srđan and Syed, Yasir Ahmed and Trattaro, Sebastiano and Caporale, Nicolo and Falk, Anna and Ofir, Rivka and Heine, Vivi M. and Chawner, Samuel J. R. A. and Rodriguez-Moreno, Antonio and van den Bree, Marianne B. M. and Testa, Giuseppe and Petrakis, Spyros and Harwood, Adrian J.",
year = "2020",
abstract = "Patients diagnosed with chromosome microdeletions or duplications, known as copy number variants (CNVs), present a unique opportunity to investigate the relationship between patient genotype and cell phenotype. CNVs have high genetic penetrance and give a good correlation between gene locus and patient clinical phenotype. This is especially effective for the study of patients with neurodevelopmental disorders (NDD), including those falling within the autism spectrum disorders (ASD). A key question is whether this correlation between genetics and clinical presentation at the level of the patient can be translated to the cell phenotypes arising from the neurodevelopment of patient induced pluripotent stem cells (iPSCs). Here, we examine how iPSCs derived from ASD patients with an associated CNV inform our understanding of the genetic and biological mechanisms underlying the aetiology of ASD. We consider selection of genetically characterised patient iPSCs; use of appropriate control lines; aspects of human neurocellular biology that can capture in vitro the patient clinical phenotype; and current limitations of patient iPSC-based studies. Finally, we consider how future research may be enhanced to maximise the utility of CNV patients for research of pathological mechanisms or therapeutic targets.",
publisher = "BMC, London",
journal = "Molecular Autism",
title = "Copy number variants (CNVs): a powerful tool for iPSC-based modelling of ASD",
number = "1",
volume = "11",
doi = "10.1186/s13229-020-00343-4"
}

Drakulić, D., Đurović, S., Syed, Y. A., Trattaro, S., Caporale, N., Falk, A., Ofir, R., Heine, V. M., Chawner, S. J. R. A., Rodriguez-Moreno, A., van den Bree, M. B. M., Testa, G., Petrakis, S.,& Harwood, A. J.. (2020). Copy number variants (CNVs): a powerful tool for iPSC-based modelling of ASD. in Molecular Autism
BMC, London., 11(1).
https://doi.org/10.1186/s13229-020-00343-4

Drakulić D, Đurović S, Syed YA, Trattaro S, Caporale N, Falk A, Ofir R, Heine VM, Chawner SJRA, Rodriguez-Moreno A, van den Bree MBM, Testa G, Petrakis S, Harwood AJ. Copy number variants (CNVs): a powerful tool for iPSC-based modelling of ASD. in Molecular Autism. 2020;11(1).
doi:10.1186/s13229-020-00343-4 .

Drakulić, Danijela, Đurović, Srđan, Syed, Yasir Ahmed, Trattaro, Sebastiano, Caporale, Nicolo, Falk, Anna, Ofir, Rivka, Heine, Vivi M., Chawner, Samuel J. R. A., Rodriguez-Moreno, Antonio, van den Bree, Marianne B. M., Testa, Giuseppe, Petrakis, Spyros, Harwood, Adrian J., "Copy number variants (CNVs): a powerful tool for iPSC-based modelling of ASD" in Molecular Autism, 11, no. 1 (2020),
https://doi.org/10.1186/s13229-020-00343-4 . .

TY  - JOUR
AU  - Jovanović, Svetlana P.
AU  - Syrgiannis, Zois
AU  - Budimir, Milica D.
AU  - Milivojević, Dusan D.
AU  - Jovanović, Dragana J.
AU  - Pavlović, Vladimir B.
AU  - Papan, Jelena M.
AU  - Bartenwerfer, Malte
AU  - Mojsin, Marija
AU  - Stevanović, Milena
AU  - Marković, Biljana M. Todorovic
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1380
AB  - Due to their low cost and possible green synthesis, high stability and resistance to photobleaching, graphene quantum dots (GQDs) can be considered as one of the class of carbon nanomaterials which may have great potential as an agent for photosensitized oxygen activation. In such a way, GQDs can be used as a theranostic agent in photodynamic therapy. In this work pristine GQDs, GQDs irradiated with gamma rays and GQDs doped with N and N, S atoms are produced using a simple, green approach. By using different techniques (AFM, HRTEM, SEM-EDS, FTIR, XRD, PL and UV-Vis) we investigated structural and optical properties of the new types of GQDs. We showed that GQDs functionalized with thiourea (GQDs-TU) completely lost the ability to produce singlet oxygen (O-1(2)) upon photoexcitation while functionalization with urea (GQDs-U) improves the capability of GQDs to produce O-1(2) upon the same conditions. Thus, presented GQDs modification with urea seems like a promising approach for the production of the efficient photosensitizer. On the opposite, GQDs-TU are efficient . OH quencher. Due to high singlet oxygen production and low cytotoxicity below 100 mu g/mL against HeLa cells, GQDs-U is a good candidate as an agent in photodynamic therapy at this concentration.
PB  - Elsevier, Amsterdam
T2  - Materials Science & Engineering C-Materials For Biological Applications
T1  - Graphene quantum dots as singlet oxygen producer or radical quencher The matter of functionalization with urea/thiourea
VL  - 109
DO  - 10.1016/j.msec.2019.110539
ER  - 

@article{
author = "Jovanović, Svetlana P. and Syrgiannis, Zois and Budimir, Milica D. and Milivojević, Dusan D. and Jovanović, Dragana J. and Pavlović, Vladimir B. and Papan, Jelena M. and Bartenwerfer, Malte and Mojsin, Marija and Stevanović, Milena and Marković, Biljana M. Todorovic",
year = "2020",
abstract = "Due to their low cost and possible green synthesis, high stability and resistance to photobleaching, graphene quantum dots (GQDs) can be considered as one of the class of carbon nanomaterials which may have great potential as an agent for photosensitized oxygen activation. In such a way, GQDs can be used as a theranostic agent in photodynamic therapy. In this work pristine GQDs, GQDs irradiated with gamma rays and GQDs doped with N and N, S atoms are produced using a simple, green approach. By using different techniques (AFM, HRTEM, SEM-EDS, FTIR, XRD, PL and UV-Vis) we investigated structural and optical properties of the new types of GQDs. We showed that GQDs functionalized with thiourea (GQDs-TU) completely lost the ability to produce singlet oxygen (O-1(2)) upon photoexcitation while functionalization with urea (GQDs-U) improves the capability of GQDs to produce O-1(2) upon the same conditions. Thus, presented GQDs modification with urea seems like a promising approach for the production of the efficient photosensitizer. On the opposite, GQDs-TU are efficient . OH quencher. Due to high singlet oxygen production and low cytotoxicity below 100 mu g/mL against HeLa cells, GQDs-U is a good candidate as an agent in photodynamic therapy at this concentration.",
publisher = "Elsevier, Amsterdam",
journal = "Materials Science & Engineering C-Materials For Biological Applications",
title = "Graphene quantum dots as singlet oxygen producer or radical quencher The matter of functionalization with urea/thiourea",
volume = "109",
doi = "10.1016/j.msec.2019.110539"
}

Jovanović, S. P., Syrgiannis, Z., Budimir, M. D., Milivojević, D. D., Jovanović, D. J., Pavlović, V. B., Papan, J. M., Bartenwerfer, M., Mojsin, M., Stevanović, M.,& Marković, B. M. T.. (2020). Graphene quantum dots as singlet oxygen producer or radical quencher The matter of functionalization with urea/thiourea. in Materials Science & Engineering C-Materials For Biological Applications
Elsevier, Amsterdam., 109.
https://doi.org/10.1016/j.msec.2019.110539

Jovanović SP, Syrgiannis Z, Budimir MD, Milivojević DD, Jovanović DJ, Pavlović VB, Papan JM, Bartenwerfer M, Mojsin M, Stevanović M, Marković BMT. Graphene quantum dots as singlet oxygen producer or radical quencher The matter of functionalization with urea/thiourea. in Materials Science & Engineering C-Materials For Biological Applications. 2020;109.
doi:10.1016/j.msec.2019.110539 .

Jovanović, Svetlana P., Syrgiannis, Zois, Budimir, Milica D., Milivojević, Dusan D., Jovanović, Dragana J., Pavlović, Vladimir B., Papan, Jelena M., Bartenwerfer, Malte, Mojsin, Marija, Stevanović, Milena, Marković, Biljana M. Todorovic, "Graphene quantum dots as singlet oxygen producer or radical quencher The matter of functionalization with urea/thiourea" in Materials Science & Engineering C-Materials For Biological Applications, 109 (2020),
https://doi.org/10.1016/j.msec.2019.110539 . .

TY  - JOUR
AU  - Dimić, Dusan S.
AU  - Marković, Zoran S.
AU  - Saso, Luciano
AU  - Avdović, Edina H.
AU  - Dorović, Jelena R.
AU  - Petrović, Isidora
AU  - Stanisavljević Ninković, Danijela
AU  - Stevanović, Milena
AU  - Potocnak, Ivan
AU  - Samol'ova, Erika
AU  - Trifunović, Srecko R.
AU  - Marković, Jasmina M. Dimitric
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1295
AB  - The newly synthesized coumarin derivative with dopamine, 3-(1-((3,4-dihydroxyphenethyDamino)ethylidene)-chroman-2,4-dione, was completely structurally characterized by X-ray crystallography. It was shown that several types of hydrogen bonds are present, which additionally stabilize the structure. The compound was tested in vitro against different cell lines, healthy human keratinocyte HaCaT, cervical squamous cell carcinoma SiHa, breast carcinoma MCF7, and hepatocellular carcinoma HepG2. Compared to control, the new derivate showed a stronger effect on both healthy and carcinoma cell lines, with the most prominent effect on the breast carcinoma MCF7 cell line. The molecular docking study, obtained for ten different conformations of the new compound, showed its inhibitory nature against CDKS protein. Lower inhibition constant, relative to one of 4-OH-coumarine, proved stronger and more numerous interactions with CDKS protein. These interactions were carefully examined for both parent molecule and derivative and explained from a structural point of view.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Synthesis and Characterization of 3-(1-((3,4-Dihydroxyphenethyl)amino)ethylidene)-chroman-2,4-dione as a Potential Antitumor Agent
VL  - 2019
DO  - 10.1155/2019/2069250
ER  - 

@article{
author = "Dimić, Dusan S. and Marković, Zoran S. and Saso, Luciano and Avdović, Edina H. and Dorović, Jelena R. and Petrović, Isidora and Stanisavljević Ninković, Danijela and Stevanović, Milena and Potocnak, Ivan and Samol'ova, Erika and Trifunović, Srecko R. and Marković, Jasmina M. Dimitric",
year = "2019",
abstract = "The newly synthesized coumarin derivative with dopamine, 3-(1-((3,4-dihydroxyphenethyDamino)ethylidene)-chroman-2,4-dione, was completely structurally characterized by X-ray crystallography. It was shown that several types of hydrogen bonds are present, which additionally stabilize the structure. The compound was tested in vitro against different cell lines, healthy human keratinocyte HaCaT, cervical squamous cell carcinoma SiHa, breast carcinoma MCF7, and hepatocellular carcinoma HepG2. Compared to control, the new derivate showed a stronger effect on both healthy and carcinoma cell lines, with the most prominent effect on the breast carcinoma MCF7 cell line. The molecular docking study, obtained for ten different conformations of the new compound, showed its inhibitory nature against CDKS protein. Lower inhibition constant, relative to one of 4-OH-coumarine, proved stronger and more numerous interactions with CDKS protein. These interactions were carefully examined for both parent molecule and derivative and explained from a structural point of view.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Synthesis and Characterization of 3-(1-((3,4-Dihydroxyphenethyl)amino)ethylidene)-chroman-2,4-dione as a Potential Antitumor Agent",
volume = "2019",
doi = "10.1155/2019/2069250"
}

Dimić, D. S., Marković, Z. S., Saso, L., Avdović, E. H., Dorović, J. R., Petrović, I., Stanisavljević Ninković, D., Stevanović, M., Potocnak, I., Samol'ova, E., Trifunović, S. R.,& Marković, J. M. D.. (2019). Synthesis and Characterization of 3-(1-((3,4-Dihydroxyphenethyl)amino)ethylidene)-chroman-2,4-dione as a Potential Antitumor Agent. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2019.
https://doi.org/10.1155/2019/2069250

Dimić DS, Marković ZS, Saso L, Avdović EH, Dorović JR, Petrović I, Stanisavljević Ninković D, Stevanović M, Potocnak I, Samol'ova E, Trifunović SR, Marković JMD. Synthesis and Characterization of 3-(1-((3,4-Dihydroxyphenethyl)amino)ethylidene)-chroman-2,4-dione as a Potential Antitumor Agent. in Oxidative Medicine and Cellular Longevity. 2019;2019.
doi:10.1155/2019/2069250 .

Dimić, Dusan S., Marković, Zoran S., Saso, Luciano, Avdović, Edina H., Dorović, Jelena R., Petrović, Isidora, Stanisavljević Ninković, Danijela, Stevanović, Milena, Potocnak, Ivan, Samol'ova, Erika, Trifunović, Srecko R., Marković, Jasmina M. Dimitric, "Synthesis and Characterization of 3-(1-((3,4-Dihydroxyphenethyl)amino)ethylidene)-chroman-2,4-dione as a Potential Antitumor Agent" in Oxidative Medicine and Cellular Longevity, 2019 (2019),
https://doi.org/10.1155/2019/2069250 . .

TY  - JOUR
AU  - Stanisavljević Ninković, Danijela
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Davidović, Slobodan
AU  - Atkinson, Michael J.
AU  - Anastasov, Nataša
AU  - Stevanović, Milena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1629
AB  - Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Radiation Biology
T1  - Radiation effects on early phase of NT2/D1 neural differentiation in vitro
EP  - 1639
IS  - 12
SP  - 1627
VL  - 95
DO  - 10.1080/09553002.2019.1665207
ER  - 

@article{
author = "Stanisavljević Ninković, Danijela and Popović, Jelena and Petrović, Isidora and Davidović, Slobodan and Atkinson, Michael J. and Anastasov, Nataša and Stevanović, Milena",
year = "2019",
abstract = "Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Radiation Biology",
title = "Radiation effects on early phase of NT2/D1 neural differentiation in vitro",
pages = "1639-1627",
number = "12",
volume = "95",
doi = "10.1080/09553002.2019.1665207"
}

Stanisavljević Ninković, D., Popović, J., Petrović, I., Davidović, S., Atkinson, M. J., Anastasov, N.,& Stevanović, M.. (2019). Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology
Taylor & Francis Ltd, Abingdon., 95(12), 1627-1639.
https://doi.org/10.1080/09553002.2019.1665207

Stanisavljević Ninković D, Popović J, Petrović I, Davidović S, Atkinson MJ, Anastasov N, Stevanović M. Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology. 2019;95(12):1627-1639.
doi:10.1080/09553002.2019.1665207 .

Stanisavljević Ninković, Danijela, Popović, Jelena, Petrović, Isidora, Davidović, Slobodan, Atkinson, Michael J., Anastasov, Nataša, Stevanović, Milena, "Radiation effects on early phase of NT2/D1 neural differentiation in vitro" in International Journal of Radiation Biology, 95, no. 12 (2019):1627-1639,
https://doi.org/10.1080/09553002.2019.1665207 . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Lazić, Andrijana
AU  - Paunesku, Tatjana
AU  - Ma, Qing
AU  - Chen, Si
AU  - Lai, Barry
AU  - Stevanović, Milena
AU  - Woloschak, Gayle E.
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1255
AB  - Chemotherapy-induced peripheral neuropathy (CIPN) is a side effect of platinum-based chemotherapy and decreases the quality of life of cancer patients. We compared neuroprotective properties of several agents using an in vitro model of terminally differentiated human cells NT2-N derived from cell line NT2/D1. Sodium azide and an active metabolite of amifostine (WR1065) increase cell viability in simultaneous treatment with cisplatin. In addition, WR1065 protects the non-dividing neurons by decreasing cisplatin caused oxidative stress and apoptosis. Accumulation of Pt in cisplatin-treated cells was heterogeneous, but the frequency and concentration of Pt in cells were lowered in the presence of WR1065 as shown by X-ray fluorescence microscopy (XFM). Transition metals accumulation accompanied Pt increase in cells; this effect was equally diminished in the presence of WR1065. To analyze possible chemical modulation of Pt-DNA bonds, we examined the platinum L-III near edge spectrum by X-ray absorption spectroscopy. The spectrum found in cisplatin-DNA samples is altered differently by the addition of either WR1065 or sodium azide. Importantly, a similar change in Pt edge spectra was noted in cells treated with cisplatin and WR1065. Therefore, amifostine should be reconsidered as a candidate for treatments that reduce or prevent CIPN.
PB  - Springer/Plenum Publishers, New York
T2  - Cellular and Molecular Neurobiology
T1  - Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro
EP  - 636
IS  - 5
SP  - 619
VL  - 39
DO  - 10.1007/s10571-019-00667-7
ER  - 

@article{
author = "Popović, Jelena and Lazić, Andrijana and Paunesku, Tatjana and Ma, Qing and Chen, Si and Lai, Barry and Stevanović, Milena and Woloschak, Gayle E.",
year = "2019",
abstract = "Chemotherapy-induced peripheral neuropathy (CIPN) is a side effect of platinum-based chemotherapy and decreases the quality of life of cancer patients. We compared neuroprotective properties of several agents using an in vitro model of terminally differentiated human cells NT2-N derived from cell line NT2/D1. Sodium azide and an active metabolite of amifostine (WR1065) increase cell viability in simultaneous treatment with cisplatin. In addition, WR1065 protects the non-dividing neurons by decreasing cisplatin caused oxidative stress and apoptosis. Accumulation of Pt in cisplatin-treated cells was heterogeneous, but the frequency and concentration of Pt in cells were lowered in the presence of WR1065 as shown by X-ray fluorescence microscopy (XFM). Transition metals accumulation accompanied Pt increase in cells; this effect was equally diminished in the presence of WR1065. To analyze possible chemical modulation of Pt-DNA bonds, we examined the platinum L-III near edge spectrum by X-ray absorption spectroscopy. The spectrum found in cisplatin-DNA samples is altered differently by the addition of either WR1065 or sodium azide. Importantly, a similar change in Pt edge spectra was noted in cells treated with cisplatin and WR1065. Therefore, amifostine should be reconsidered as a candidate for treatments that reduce or prevent CIPN.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Cellular and Molecular Neurobiology",
title = "Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro",
pages = "636-619",
number = "5",
volume = "39",
doi = "10.1007/s10571-019-00667-7"
}

Popović, J., Lazić, A., Paunesku, T., Ma, Q., Chen, S., Lai, B., Stevanović, M.,& Woloschak, G. E.. (2019). Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro. in Cellular and Molecular Neurobiology
Springer/Plenum Publishers, New York., 39(5), 619-636.
https://doi.org/10.1007/s10571-019-00667-7

Popović J, Lazić A, Paunesku T, Ma Q, Chen S, Lai B, Stevanović M, Woloschak GE. Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro. in Cellular and Molecular Neurobiology. 2019;39(5):619-636.
doi:10.1007/s10571-019-00667-7 .

Popović, Jelena, Lazić, Andrijana, Paunesku, Tatjana, Ma, Qing, Chen, Si, Lai, Barry, Stevanović, Milena, Woloschak, Gayle E., "Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro" in Cellular and Molecular Neurobiology, 39, no. 5 (2019):619-636,
https://doi.org/10.1007/s10571-019-00667-7 . .

TY  - JOUR
AU  - Stanisavljević Ninković, Danijela
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Davidović, Slobodan
AU  - Atkinson, Michael J.
AU  - Anastasov, Nataša
AU  - Stevanović, Milena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1206
AB  - Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Radiation Biology
T1  - Radiation effects on early phase of NT2/D1 neural differentiation in vitro
EP  - 1639
IS  - 12
SP  - 1627
VL  - 95
DO  - 10.1080/09553002.2019.1665207
ER  - 

@article{
author = "Stanisavljević Ninković, Danijela and Popović, Jelena and Petrović, Isidora and Davidović, Slobodan and Atkinson, Michael J. and Anastasov, Nataša and Stevanović, Milena",
year = "2019",
abstract = "Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Radiation Biology",
title = "Radiation effects on early phase of NT2/D1 neural differentiation in vitro",
pages = "1639-1627",
number = "12",
volume = "95",
doi = "10.1080/09553002.2019.1665207"
}

Stanisavljević Ninković, D., Popović, J., Petrović, I., Davidović, S., Atkinson, M. J., Anastasov, N.,& Stevanović, M.. (2019). Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology
Taylor & Francis Ltd, Abingdon., 95(12), 1627-1639.
https://doi.org/10.1080/09553002.2019.1665207

Stanisavljević Ninković D, Popović J, Petrović I, Davidović S, Atkinson MJ, Anastasov N, Stevanović M. Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology. 2019;95(12):1627-1639.
doi:10.1080/09553002.2019.1665207 .

Stanisavljević Ninković, Danijela, Popović, Jelena, Petrović, Isidora, Davidović, Slobodan, Atkinson, Michael J., Anastasov, Nataša, Stevanović, Milena, "Radiation effects on early phase of NT2/D1 neural differentiation in vitro" in International Journal of Radiation Biology, 95, no. 12 (2019):1627-1639,
https://doi.org/10.1080/09553002.2019.1665207 . .

TY  - JOUR
AU  - Vicentić, Jelena Marjanovic
AU  - Drakulić, Danijela
AU  - Garcia, Idoia
AU  - Vuković, Vladanka
AU  - Aldaz, Paula
AU  - Puskas, Nela
AU  - Nikolić, Igor
AU  - Tasić, Goran
AU  - Raicević, Savo
AU  - Garros-Regulez, Laura
AU  - Sampron, Nicolas
AU  - Atkinson, Michael J.
AU  - Anastasov, Nataša
AU  - Matheu, Ander
AU  - Stevanović, Milena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1285
AB  - PurposeGlioblastoma is the most common and lethal adult brain tumor. Despite current therapeutic strategies, including surgery, radiation and chemotherapy, the median survival of glioblastoma patients is 15months. The development of this tumor depends on a sub-population of glioblastoma stem cells governing tumor propagation and therapy resistance. SOX3 plays a role in both normal neural development and carcinogenesis. However, little is known about its role in glioblastoma. Thus, the aim of this work was to elucidate the role of SOX3 in glioblastoma.MethodsSOX3 expression was assessed using real-time quantitative PCR (RT-qPCR), Western blotting and immunohistochemistry. MTT, immunocytochemistry and Transwell assays were used to evaluate the effects of exogenous SOX3 overexpression on the viability, proliferation, migration and invasion of glioblastoma cells, respectively. The expression of Hedgehog signaling pathway components and autophagy markers was assessed using RT-qPCR and Western blot analyses, respectively.ResultsHigher levels of SOX3 expression were detected in a subset of primary glioblastoma samples compared to those in non-tumoral brain tissues. Exogenous overexpression of this gene was found to increase the proliferation, viability, migration and invasion of glioblastoma cells. We also found that SOX3 up-regulation was accompanied by an enhanced activity of the Hedgehog signaling pathway and by suppression of autophagy in glioblastoma cells. Additionally, we found that SOX3 expression was elevated in patient-derived glioblastoma stem cells, as well as in oncospheres derived from glioblastoma cell lines, compared to their differentiated counterparts, implying that SOX3 expression is associated with the undifferentiated state of glioblastoma cells.ConclusionFrom our data we conclude that SOX3 can promote the malignant behavior of glioblastoma cells.
PB  - Springer, Dordrecht
T2  - Cellular Oncology
T1  - SOX3 can promote the malignant behavior of glioblastoma cells
EP  - 54
IS  - 1
SP  - 41
VL  - 42
DO  - 10.1007/s13402-018-0405-5
ER  - 

@article{
author = "Vicentić, Jelena Marjanovic and Drakulić, Danijela and Garcia, Idoia and Vuković, Vladanka and Aldaz, Paula and Puskas, Nela and Nikolić, Igor and Tasić, Goran and Raicević, Savo and Garros-Regulez, Laura and Sampron, Nicolas and Atkinson, Michael J. and Anastasov, Nataša and Matheu, Ander and Stevanović, Milena",
year = "2019",
abstract = "PurposeGlioblastoma is the most common and lethal adult brain tumor. Despite current therapeutic strategies, including surgery, radiation and chemotherapy, the median survival of glioblastoma patients is 15months. The development of this tumor depends on a sub-population of glioblastoma stem cells governing tumor propagation and therapy resistance. SOX3 plays a role in both normal neural development and carcinogenesis. However, little is known about its role in glioblastoma. Thus, the aim of this work was to elucidate the role of SOX3 in glioblastoma.MethodsSOX3 expression was assessed using real-time quantitative PCR (RT-qPCR), Western blotting and immunohistochemistry. MTT, immunocytochemistry and Transwell assays were used to evaluate the effects of exogenous SOX3 overexpression on the viability, proliferation, migration and invasion of glioblastoma cells, respectively. The expression of Hedgehog signaling pathway components and autophagy markers was assessed using RT-qPCR and Western blot analyses, respectively.ResultsHigher levels of SOX3 expression were detected in a subset of primary glioblastoma samples compared to those in non-tumoral brain tissues. Exogenous overexpression of this gene was found to increase the proliferation, viability, migration and invasion of glioblastoma cells. We also found that SOX3 up-regulation was accompanied by an enhanced activity of the Hedgehog signaling pathway and by suppression of autophagy in glioblastoma cells. Additionally, we found that SOX3 expression was elevated in patient-derived glioblastoma stem cells, as well as in oncospheres derived from glioblastoma cell lines, compared to their differentiated counterparts, implying that SOX3 expression is associated with the undifferentiated state of glioblastoma cells.ConclusionFrom our data we conclude that SOX3 can promote the malignant behavior of glioblastoma cells.",
publisher = "Springer, Dordrecht",
journal = "Cellular Oncology",
title = "SOX3 can promote the malignant behavior of glioblastoma cells",
pages = "54-41",
number = "1",
volume = "42",
doi = "10.1007/s13402-018-0405-5"
}

Vicentić, J. M., Drakulić, D., Garcia, I., Vuković, V., Aldaz, P., Puskas, N., Nikolić, I., Tasić, G., Raicević, S., Garros-Regulez, L., Sampron, N., Atkinson, M. J., Anastasov, N., Matheu, A.,& Stevanović, M.. (2019). SOX3 can promote the malignant behavior of glioblastoma cells. in Cellular Oncology
Springer, Dordrecht., 42(1), 41-54.
https://doi.org/10.1007/s13402-018-0405-5

Vicentić JM, Drakulić D, Garcia I, Vuković V, Aldaz P, Puskas N, Nikolić I, Tasić G, Raicević S, Garros-Regulez L, Sampron N, Atkinson MJ, Anastasov N, Matheu A, Stevanović M. SOX3 can promote the malignant behavior of glioblastoma cells. in Cellular Oncology. 2019;42(1):41-54.
doi:10.1007/s13402-018-0405-5 .

Vicentić, Jelena Marjanovic, Drakulić, Danijela, Garcia, Idoia, Vuković, Vladanka, Aldaz, Paula, Puskas, Nela, Nikolić, Igor, Tasić, Goran, Raicević, Savo, Garros-Regulez, Laura, Sampron, Nicolas, Atkinson, Michael J., Anastasov, Nataša, Matheu, Ander, Stevanović, Milena, "SOX3 can promote the malignant behavior of glioblastoma cells" in Cellular Oncology, 42, no. 1 (2019):41-54,
https://doi.org/10.1007/s13402-018-0405-5 . .

TY  - JOUR
AU  - Todorović, Nevena
AU  - Micić, Bojana
AU  - Schwirtlich, Marija
AU  - Stevanović, Milena
AU  - Filipović, Dragana
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1253
AB  - Dysregulation of GABAergic system is becoming increasingly associated with depression, psychiatric disorder that imposes severe clinical, social and economic burden. Special attention is paid to the fast-spiking parvalbumin-positive (PV+) interneurons, GABAergic neurons which are highly susceptible to redox dysregulation and oxidative stress and implicated in a variety of psychiatric diseases. Here we analyzed the number of PV+ and cleaved caspase-3-positive (CC3+) cells in the rat medial prefrontal cortical (mPFC) subregions following chronic social isolation (CSIS), an animal model of depression and schizophrenia. Also, we examined potential protective effects of antidepressant fluoxetine (FLX) and atypical antipsychotic clozapine (CLZ) on the number of these cells in mPFC subregions, when applied parallel with CSIS in doses that correspond to therapeutically effective ones in patients. Immunofluorescence analysis revealed decreased number of PV+ cells in cingulate cortex area 1, prelimbic area (PrL), infralimbic area (IL) and dorsal peduncular cortex of the mPFC in isolated rats, which coincided with depressive- and anxiety-like behaviors. In addition, CSIS-induced increase in the number of CC3+ cells was detected in aforementioned subregions of mPFC. Treatments with either FLX or CLZ prevented behavioral changes, decrease in PV+ and increase in CC3+ cell numbers in PrL and IL subregions in isolated rats. These results indicate the importance of intact GABAergic signaling in these areas for resistance against CSIS-induced behavioral changes, as well as subregion-specific protective effects of FLX and CLZ in mPFC of CSIS rats.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Neuroscience
T1  - Subregion-specific Protective Effects of Fluoxetine and Clozapine on Parvalbumin Expression in Medial Prefrontal Cortex of Chronically Isolated Rats
EP  - 35
SP  - 24
VL  - 396
DO  - 10.1016/j.neuroscience.2018.11.008
ER  - 

@article{
author = "Todorović, Nevena and Micić, Bojana and Schwirtlich, Marija and Stevanović, Milena and Filipović, Dragana",
year = "2019",
abstract = "Dysregulation of GABAergic system is becoming increasingly associated with depression, psychiatric disorder that imposes severe clinical, social and economic burden. Special attention is paid to the fast-spiking parvalbumin-positive (PV+) interneurons, GABAergic neurons which are highly susceptible to redox dysregulation and oxidative stress and implicated in a variety of psychiatric diseases. Here we analyzed the number of PV+ and cleaved caspase-3-positive (CC3+) cells in the rat medial prefrontal cortical (mPFC) subregions following chronic social isolation (CSIS), an animal model of depression and schizophrenia. Also, we examined potential protective effects of antidepressant fluoxetine (FLX) and atypical antipsychotic clozapine (CLZ) on the number of these cells in mPFC subregions, when applied parallel with CSIS in doses that correspond to therapeutically effective ones in patients. Immunofluorescence analysis revealed decreased number of PV+ cells in cingulate cortex area 1, prelimbic area (PrL), infralimbic area (IL) and dorsal peduncular cortex of the mPFC in isolated rats, which coincided with depressive- and anxiety-like behaviors. In addition, CSIS-induced increase in the number of CC3+ cells was detected in aforementioned subregions of mPFC. Treatments with either FLX or CLZ prevented behavioral changes, decrease in PV+ and increase in CC3+ cell numbers in PrL and IL subregions in isolated rats. These results indicate the importance of intact GABAergic signaling in these areas for resistance against CSIS-induced behavioral changes, as well as subregion-specific protective effects of FLX and CLZ in mPFC of CSIS rats.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Neuroscience",
title = "Subregion-specific Protective Effects of Fluoxetine and Clozapine on Parvalbumin Expression in Medial Prefrontal Cortex of Chronically Isolated Rats",
pages = "35-24",
volume = "396",
doi = "10.1016/j.neuroscience.2018.11.008"
}

Todorović, N., Micić, B., Schwirtlich, M., Stevanović, M.,& Filipović, D.. (2019). Subregion-specific Protective Effects of Fluoxetine and Clozapine on Parvalbumin Expression in Medial Prefrontal Cortex of Chronically Isolated Rats. in Neuroscience
Pergamon-Elsevier Science Ltd, Oxford., 396, 24-35.
https://doi.org/10.1016/j.neuroscience.2018.11.008

Todorović N, Micić B, Schwirtlich M, Stevanović M, Filipović D. Subregion-specific Protective Effects of Fluoxetine and Clozapine on Parvalbumin Expression in Medial Prefrontal Cortex of Chronically Isolated Rats. in Neuroscience. 2019;396:24-35.
doi:10.1016/j.neuroscience.2018.11.008 .

Todorović, Nevena, Micić, Bojana, Schwirtlich, Marija, Stevanović, Milena, Filipović, Dragana, "Subregion-specific Protective Effects of Fluoxetine and Clozapine on Parvalbumin Expression in Medial Prefrontal Cortex of Chronically Isolated Rats" in Neuroscience, 396 (2019):24-35,
https://doi.org/10.1016/j.neuroscience.2018.11.008 . .

TY  - JOUR
AU  - Marković, Zoran M.
AU  - Jovanović, Svetlana P.
AU  - Masković, Pavle Z.
AU  - Mojsin, Marija
AU  - Stevanović, Milena
AU  - Danko, Martin
AU  - Micusik, Matej
AU  - Jovanović, Dragana J.
AU  - Kleinova, Angela
AU  - Spitalsky, Zdeno
AU  - Pavlović, Vladimir B.
AU  - Marković, Biljana M. Todorovic
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1221
AB  - Photoactive materials called photosensitizers can be used for treatment of different types of cancer in combination with light source. In this paper, we have investigated pro-oxidant and antioxidant potentials of four graphene based nanomaterials (graphene oxide-GO, graphene quantum dots-GQDs, carbon quantum dots-CQDs and N-doped carbon quantum dots-N-CQDs) depending on the presence/absence of visible light source. Structural and optical properties of these materials and their potentials for reactive oxygen species generation/quenching are investigated by applying different microscopy and spectroscopy techniques (transmission electron microscopy, FTIR, UV-Vis, photoluminescence, electron paramagnetic resonance). Results show that all types of quantum dots has pro-oxidant and antioxidant potentials whereas GO demonstrated only moderate antioxidant effect. The best free radical scavenger is CQDs sample in the absence of light. CQDs are the best singlet oxygen generator under blue light irradiation as well. To check photo-cytotoxicity of these materials, photo-cytotoxic concentrations of the GO, GQDs, CQDs and N-CQDs were determined for three cellular lines: human rhabdomyosarcoma (RD), cell line derived from human cervix carcinoma Hep2c (HeLa) and fibroblast cell line from murine (L2OB). Cytotoxicity test has indicated that all samples are much less photocytotoxic than cis-diamminedichloroplatinum (cis-DPP). The production method and doping of quantum dots affect the photodynamic activity of tested samples very much.
PB  - Elsevier Science Sa, Lausanne
T2  - Journal of Photochemistry and Photobiology B-Biology
T1  - Graphene oxide size and structure pro-oxidant and antioxidant activity and photoinduced cytotoxicity relation on three cancer cell lines
VL  - 200
DO  - 10.1016/j.jphotobiol.2019.111647
ER  - 

@article{
author = "Marković, Zoran M. and Jovanović, Svetlana P. and Masković, Pavle Z. and Mojsin, Marija and Stevanović, Milena and Danko, Martin and Micusik, Matej and Jovanović, Dragana J. and Kleinova, Angela and Spitalsky, Zdeno and Pavlović, Vladimir B. and Marković, Biljana M. Todorovic",
year = "2019",
abstract = "Photoactive materials called photosensitizers can be used for treatment of different types of cancer in combination with light source. In this paper, we have investigated pro-oxidant and antioxidant potentials of four graphene based nanomaterials (graphene oxide-GO, graphene quantum dots-GQDs, carbon quantum dots-CQDs and N-doped carbon quantum dots-N-CQDs) depending on the presence/absence of visible light source. Structural and optical properties of these materials and their potentials for reactive oxygen species generation/quenching are investigated by applying different microscopy and spectroscopy techniques (transmission electron microscopy, FTIR, UV-Vis, photoluminescence, electron paramagnetic resonance). Results show that all types of quantum dots has pro-oxidant and antioxidant potentials whereas GO demonstrated only moderate antioxidant effect. The best free radical scavenger is CQDs sample in the absence of light. CQDs are the best singlet oxygen generator under blue light irradiation as well. To check photo-cytotoxicity of these materials, photo-cytotoxic concentrations of the GO, GQDs, CQDs and N-CQDs were determined for three cellular lines: human rhabdomyosarcoma (RD), cell line derived from human cervix carcinoma Hep2c (HeLa) and fibroblast cell line from murine (L2OB). Cytotoxicity test has indicated that all samples are much less photocytotoxic than cis-diamminedichloroplatinum (cis-DPP). The production method and doping of quantum dots affect the photodynamic activity of tested samples very much.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Journal of Photochemistry and Photobiology B-Biology",
title = "Graphene oxide size and structure pro-oxidant and antioxidant activity and photoinduced cytotoxicity relation on three cancer cell lines",
volume = "200",
doi = "10.1016/j.jphotobiol.2019.111647"
}

Marković, Z. M., Jovanović, S. P., Masković, P. Z., Mojsin, M., Stevanović, M., Danko, M., Micusik, M., Jovanović, D. J., Kleinova, A., Spitalsky, Z., Pavlović, V. B.,& Marković, B. M. T.. (2019). Graphene oxide size and structure pro-oxidant and antioxidant activity and photoinduced cytotoxicity relation on three cancer cell lines. in Journal of Photochemistry and Photobiology B-Biology
Elsevier Science Sa, Lausanne., 200.
https://doi.org/10.1016/j.jphotobiol.2019.111647

Marković ZM, Jovanović SP, Masković PZ, Mojsin M, Stevanović M, Danko M, Micusik M, Jovanović DJ, Kleinova A, Spitalsky Z, Pavlović VB, Marković BMT. Graphene oxide size and structure pro-oxidant and antioxidant activity and photoinduced cytotoxicity relation on three cancer cell lines. in Journal of Photochemistry and Photobiology B-Biology. 2019;200.
doi:10.1016/j.jphotobiol.2019.111647 .

Marković, Zoran M., Jovanović, Svetlana P., Masković, Pavle Z., Mojsin, Marija, Stevanović, Milena, Danko, Martin, Micusik, Matej, Jovanović, Dragana J., Kleinova, Angela, Spitalsky, Zdeno, Pavlović, Vladimir B., Marković, Biljana M. Todorovic, "Graphene oxide size and structure pro-oxidant and antioxidant activity and photoinduced cytotoxicity relation on three cancer cell lines" in Journal of Photochemistry and Photobiology B-Biology, 200 (2019),
https://doi.org/10.1016/j.jphotobiol.2019.111647 . .

TY  - JOUR
AU  - Tošić, Nataša
AU  - Petrović, Isidora
AU  - Kovačević Grujičić, Nataša
AU  - Davidović, Slobodan
AU  - Virijević, Marijana
AU  - Suvajdžić-Vuković, Nada
AU  - Pavlović, Sonja
AU  - Stevanović, Milena
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1196
AB  - Aberrant expression of different SOX (SRY-related high mobility group (HMG) box) genes has been observed in number of tumors but, little is known about their expression patterns in hematological malignancies, especially in acute myeloid leukemia (AML). In this study we investigated SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in 50 de novo adult AML patients and correlated our findings with known clinical and molecular prognostic markers of the disease. We have found that these genes are overexpressed in 10-22% of patients and preliminary findings suggest that high expression level of these genes may have prognostic significance in AML patients. This is the first study focused on examining the expression level of SOX2, SOX3, SOX11, SOX14 and SOX18 genes in AML patients. Although this is a relatively limited study, initial findings indicate the need for further investigation of these genes, their potential roles in leukemia pathogenesis as well as prognosis in AML patients.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Leukemia Research
T1  - Prognostic significance of SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in adult de novo acute myeloid leukemia
EP  - 38
SP  - 32
VL  - 67
DO  - 10.1016/j.leukres.2018.02.001
ER  - 

@article{
author = "Tošić, Nataša and Petrović, Isidora and Kovačević Grujičić, Nataša and Davidović, Slobodan and Virijević, Marijana and Suvajdžić-Vuković, Nada and Pavlović, Sonja and Stevanović, Milena",
year = "2018",
abstract = "Aberrant expression of different SOX (SRY-related high mobility group (HMG) box) genes has been observed in number of tumors but, little is known about their expression patterns in hematological malignancies, especially in acute myeloid leukemia (AML). In this study we investigated SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in 50 de novo adult AML patients and correlated our findings with known clinical and molecular prognostic markers of the disease. We have found that these genes are overexpressed in 10-22% of patients and preliminary findings suggest that high expression level of these genes may have prognostic significance in AML patients. This is the first study focused on examining the expression level of SOX2, SOX3, SOX11, SOX14 and SOX18 genes in AML patients. Although this is a relatively limited study, initial findings indicate the need for further investigation of these genes, their potential roles in leukemia pathogenesis as well as prognosis in AML patients.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Leukemia Research",
title = "Prognostic significance of SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in adult de novo acute myeloid leukemia",
pages = "38-32",
volume = "67",
doi = "10.1016/j.leukres.2018.02.001"
}

Tošić, N., Petrović, I., Kovačević Grujičić, N., Davidović, S., Virijević, M., Suvajdžić-Vuković, N., Pavlović, S.,& Stevanović, M.. (2018). Prognostic significance of SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in adult de novo acute myeloid leukemia. in Leukemia Research
Pergamon-Elsevier Science Ltd, Oxford., 67, 32-38.
https://doi.org/10.1016/j.leukres.2018.02.001

Tošić N, Petrović I, Kovačević Grujičić N, Davidović S, Virijević M, Suvajdžić-Vuković N, Pavlović S, Stevanović M. Prognostic significance of SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in adult de novo acute myeloid leukemia. in Leukemia Research. 2018;67:32-38.
doi:10.1016/j.leukres.2018.02.001 .

Tošić, Nataša, Petrović, Isidora, Kovačević Grujičić, Nataša, Davidović, Slobodan, Virijević, Marijana, Suvajdžić-Vuković, Nada, Pavlović, Sonja, Stevanović, Milena, "Prognostic significance of SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in adult de novo acute myeloid leukemia" in Leukemia Research, 67 (2018):32-38,
https://doi.org/10.1016/j.leukres.2018.02.001 . .

TY  - JOUR
AU  - Ostojić, Sergej M.
AU  - Mojsin, Marija
AU  - Drid, Patrik
AU  - Vranes, Milan
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1145
AB  - Background/Aims: Guanidinoacetic acid (GAA) is an experimental dietary additive and has been reported to induce methyl depletion when provided by the diet. However, no study evaluated whether supplemental GAA affects DNA methylation, a critical epigenetic process for genome regulation. Methods: In this open-label, repeated-measure interventional trial, we evaluated the impact of 12 weeks of GAA supplementation on global DNA methylation in 14 healthy participants (8 women and 6 men, age 22.2 +/- 2.3 years, body mass index 24.8 +/- 5.7). Results: Dietary provision of GAA had no effect on global DNA methylation, with 5-methylcytosine (m5C) nonsignificantly increased by 13.4% at postadministration when averaged across participants (95% confidence interval -5.5 to 32.3; p = 0.26). Notable DNA hypomethylation (corresponding to a 5% drop in m5C) was found in 3 of 14 participants at follow-up. Conclusion: Global DNA methylation seems to be unaltered by dietary provision of 3 g of GAA per day for 12 weeks in healthy men and women.
PB  - Karger, Basel
T2  - Lifestyle Genomics
T1  - Does Dietary Provision of Guanidinoacetic Acid Induce Global DNA Hypomethylation in Healthy Men and Women?
EP  - 18
IS  - 1
SP  - 16
VL  - 11
DO  - 10.1159/000487336
ER  - 

@article{
author = "Ostojić, Sergej M. and Mojsin, Marija and Drid, Patrik and Vranes, Milan",
year = "2018",
abstract = "Background/Aims: Guanidinoacetic acid (GAA) is an experimental dietary additive and has been reported to induce methyl depletion when provided by the diet. However, no study evaluated whether supplemental GAA affects DNA methylation, a critical epigenetic process for genome regulation. Methods: In this open-label, repeated-measure interventional trial, we evaluated the impact of 12 weeks of GAA supplementation on global DNA methylation in 14 healthy participants (8 women and 6 men, age 22.2 +/- 2.3 years, body mass index 24.8 +/- 5.7). Results: Dietary provision of GAA had no effect on global DNA methylation, with 5-methylcytosine (m5C) nonsignificantly increased by 13.4% at postadministration when averaged across participants (95% confidence interval -5.5 to 32.3; p = 0.26). Notable DNA hypomethylation (corresponding to a 5% drop in m5C) was found in 3 of 14 participants at follow-up. Conclusion: Global DNA methylation seems to be unaltered by dietary provision of 3 g of GAA per day for 12 weeks in healthy men and women.",
publisher = "Karger, Basel",
journal = "Lifestyle Genomics",
title = "Does Dietary Provision of Guanidinoacetic Acid Induce Global DNA Hypomethylation in Healthy Men and Women?",
pages = "18-16",
number = "1",
volume = "11",
doi = "10.1159/000487336"
}

Ostojić, S. M., Mojsin, M., Drid, P.,& Vranes, M.. (2018). Does Dietary Provision of Guanidinoacetic Acid Induce Global DNA Hypomethylation in Healthy Men and Women?. in Lifestyle Genomics
Karger, Basel., 11(1), 16-18.
https://doi.org/10.1159/000487336

Ostojić SM, Mojsin M, Drid P, Vranes M. Does Dietary Provision of Guanidinoacetic Acid Induce Global DNA Hypomethylation in Healthy Men and Women?. in Lifestyle Genomics. 2018;11(1):16-18.
doi:10.1159/000487336 .

Ostojić, Sergej M., Mojsin, Marija, Drid, Patrik, Vranes, Milan, "Does Dietary Provision of Guanidinoacetic Acid Induce Global DNA Hypomethylation in Healthy Men and Women?" in Lifestyle Genomics, 11, no. 1 (2018):16-18,
https://doi.org/10.1159/000487336 . .

TY  - JOUR
AU  - Videnović, Milica
AU  - Mojsin, Marija
AU  - Stevanović, Milena
AU  - Opsenica, Igor
AU  - Srdić-Rajić, Tatjana
AU  - Solaja, Bogdan
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1148
AB  - A series of new benzothiazole-based carbamates and amides were synthesized and their antiproliferative activity was determined. Derivatives with profound activity were identified and further investigated for their possible mechanism of action. It was found that these compounds induce specific apoptosis, G2/M cell cycle arrest and decrease ROS level in MCF-7 human breast cancer cell line. Moreover, sub-micromolar antiproliferative activity of examined carbamates against NT2/D1 testicular embryonal carcinoma was shown. The most potent derivatives strongly inhibited NT2/D1 cell migration and invasiveness.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - European Journal of Medicinal Chemistry
T1  - Benzothiazole carbamates and amides as antiproliferative species
EP  - 1114
SP  - 1096
VL  - 157
DO  - 10.1016/j.ejmech.2018.08.067
ER  - 

@article{
author = "Videnović, Milica and Mojsin, Marija and Stevanović, Milena and Opsenica, Igor and Srdić-Rajić, Tatjana and Solaja, Bogdan",
year = "2018",
abstract = "A series of new benzothiazole-based carbamates and amides were synthesized and their antiproliferative activity was determined. Derivatives with profound activity were identified and further investigated for their possible mechanism of action. It was found that these compounds induce specific apoptosis, G2/M cell cycle arrest and decrease ROS level in MCF-7 human breast cancer cell line. Moreover, sub-micromolar antiproliferative activity of examined carbamates against NT2/D1 testicular embryonal carcinoma was shown. The most potent derivatives strongly inhibited NT2/D1 cell migration and invasiveness.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "European Journal of Medicinal Chemistry",
title = "Benzothiazole carbamates and amides as antiproliferative species",
pages = "1114-1096",
volume = "157",
doi = "10.1016/j.ejmech.2018.08.067"
}

Videnović, M., Mojsin, M., Stevanović, M., Opsenica, I., Srdić-Rajić, T.,& Solaja, B.. (2018). Benzothiazole carbamates and amides as antiproliferative species. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 157, 1096-1114.
https://doi.org/10.1016/j.ejmech.2018.08.067

Videnović M, Mojsin M, Stevanović M, Opsenica I, Srdić-Rajić T, Solaja B. Benzothiazole carbamates and amides as antiproliferative species. in European Journal of Medicinal Chemistry. 2018;157:1096-1114.
doi:10.1016/j.ejmech.2018.08.067 .

Videnović, Milica, Mojsin, Marija, Stevanović, Milena, Opsenica, Igor, Srdić-Rajić, Tatjana, Solaja, Bogdan, "Benzothiazole carbamates and amides as antiproliferative species" in European Journal of Medicinal Chemistry, 157 (2018):1096-1114,
https://doi.org/10.1016/j.ejmech.2018.08.067 . .

TY  - JOUR
AU  - Garcia, Idoia
AU  - Aldaregia, Juncal
AU  - Vicentić, Jelena Marjanovic
AU  - Aldaz, Paula
AU  - Moreno-Cugnon, Leire
AU  - Torres-Bayona, Sergio
AU  - Carrasco-Garcia, Estefania
AU  - Garros-Regulez, Laura
AU  - Egana, Larraitz
AU  - Rubio, Angel
AU  - Pollard, Steven
AU  - Stevanović, Milena
AU  - Sampron, Nicolas
AU  - Matheu, Ander
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1083
AB  - Glioblastoma remains the most common and deadliest type of brain tumor and contains a population of self-renewing, highly tumorigenic glioma stem cells (GSCs), which contributes to tumor initiation and treatment resistance. Developmental programs participating in tissue development and homeostasis re-emerge in GSCs, supporting the development and progression of glioblastoma. SOX1 plays an important role in neural development and neural progenitor pool maintenance. Its impact on glioblastoma remains largely unknown. In this study, we have found that high levels of SOX1 observed in a subset of patients correlate with lower overall survival. At the cellular level, SOX1 expression is elevated in patient-derived GSCs and it is also higher in oncosphere culture compared to differentiation conditions in conventional glioblastoma cell lines. Moreover, genetic inhibition of SOX1 in patient-derived GSCs and conventional cell lines decreases self-renewal and proliferative capacity in vitro and tumor initiation and growth in vivo. Contrarily, SOX1 over-expression moderately promotes self-renewal and proliferation in GSCs. These functions seem to be independent of its activity as Wnt/beta-catenin signaling regulator. In summary, these results identify a functional role for SOX1 in regulating glioma cell heterogeneity and plasticity, and suggest SOX1 as a potential target in the GSC population in glioblastoma.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Oncogenic activity of SOX1 in glioblastoma
VL  - 7
DO  - 10.1038/srep46575
ER  - 

@article{
author = "Garcia, Idoia and Aldaregia, Juncal and Vicentić, Jelena Marjanovic and Aldaz, Paula and Moreno-Cugnon, Leire and Torres-Bayona, Sergio and Carrasco-Garcia, Estefania and Garros-Regulez, Laura and Egana, Larraitz and Rubio, Angel and Pollard, Steven and Stevanović, Milena and Sampron, Nicolas and Matheu, Ander",
year = "2017",
abstract = "Glioblastoma remains the most common and deadliest type of brain tumor and contains a population of self-renewing, highly tumorigenic glioma stem cells (GSCs), which contributes to tumor initiation and treatment resistance. Developmental programs participating in tissue development and homeostasis re-emerge in GSCs, supporting the development and progression of glioblastoma. SOX1 plays an important role in neural development and neural progenitor pool maintenance. Its impact on glioblastoma remains largely unknown. In this study, we have found that high levels of SOX1 observed in a subset of patients correlate with lower overall survival. At the cellular level, SOX1 expression is elevated in patient-derived GSCs and it is also higher in oncosphere culture compared to differentiation conditions in conventional glioblastoma cell lines. Moreover, genetic inhibition of SOX1 in patient-derived GSCs and conventional cell lines decreases self-renewal and proliferative capacity in vitro and tumor initiation and growth in vivo. Contrarily, SOX1 over-expression moderately promotes self-renewal and proliferation in GSCs. These functions seem to be independent of its activity as Wnt/beta-catenin signaling regulator. In summary, these results identify a functional role for SOX1 in regulating glioma cell heterogeneity and plasticity, and suggest SOX1 as a potential target in the GSC population in glioblastoma.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Oncogenic activity of SOX1 in glioblastoma",
volume = "7",
doi = "10.1038/srep46575"
}

Garcia, I., Aldaregia, J., Vicentić, J. M., Aldaz, P., Moreno-Cugnon, L., Torres-Bayona, S., Carrasco-Garcia, E., Garros-Regulez, L., Egana, L., Rubio, A., Pollard, S., Stevanović, M., Sampron, N.,& Matheu, A.. (2017). Oncogenic activity of SOX1 in glioblastoma. in Scientific Reports
Nature Publishing Group, London., 7.
https://doi.org/10.1038/srep46575

Garcia I, Aldaregia J, Vicentić JM, Aldaz P, Moreno-Cugnon L, Torres-Bayona S, Carrasco-Garcia E, Garros-Regulez L, Egana L, Rubio A, Pollard S, Stevanović M, Sampron N, Matheu A. Oncogenic activity of SOX1 in glioblastoma. in Scientific Reports. 2017;7.
doi:10.1038/srep46575 .

Garcia, Idoia, Aldaregia, Juncal, Vicentić, Jelena Marjanovic, Aldaz, Paula, Moreno-Cugnon, Leire, Torres-Bayona, Sergio, Carrasco-Garcia, Estefania, Garros-Regulez, Laura, Egana, Larraitz, Rubio, Angel, Pollard, Steven, Stevanović, Milena, Sampron, Nicolas, Matheu, Ander, "Oncogenic activity of SOX1 in glioblastoma" in Scientific Reports, 7 (2017),
https://doi.org/10.1038/srep46575 . .

TY  - JOUR
AU  - Stojković, Dejan S.
AU  - Kovačević Grujičić, Nataša
AU  - Reis, Filipa S.
AU  - Davidović, Slobodan
AU  - Barros, Lillian
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Pavić, Aleksandar
AU  - Glamoclija, Jasmina
AU  - Cirić, Ana
AU  - Stevanović, Milena
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1051
AB  - Wild Meripilus giganteus Karst belongs to the order Polyporales, in which some members are known to possess a wide range of pharmacological properties. M. giganteus showed to be rich in carbohydrates (74.49 g/100 g) and proteins (15.94 g/100 g), presenting low fat content (1.51 g/100 g). Chemical composition was determined by using chromatographic techniques. Also, various bioactive compounds were detected including all four tocopherol isoforms with delta- and gamma-tocopherols being predominant (123.35 and 77.80 mu g/100 g, respectively); five organic acids (oxalic, malic, quinic, citric and fumaric acids) with predominant malic acid (3.17 g/100 g); and three phenolic acids and related compounds (p-hydroxybenzoic, p-coumaric and cinnamic acids; 1010, 2420 and 340 mu g/100 g, respectively). M. giganteus methanolic extract exhibited antioxidant activity tested by five different assays with the strongest potential in TBARS assay (EC50 0.31 mg/mL); and antimicrobial activities (MIC/MBC 0.0125-5 mg/mL; MIC/MFC 0.025-0.4 mg/mL). Furthermore, treatment of cervical carcinoma cell line (HeLa) led to reduction in cell's viability in MTT assay (lC(50) 0.41 mg/mL after 48 h), induced process of apoptosis and inhibited cell's migration in vitro. The analysed extract was not toxic for zebrafish embryos (at 0.5 mg/mL), indicating its biosafety and potential application as a dietary supplement in chemoprevention.
PB  - Elsevier Science Bv, Amsterdam
T2  - Lwt-Food Science and Technology
T1  - Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract
EP  - 462
SP  - 454
VL  - 79
DO  - 10.1016/j.lwt.2017.01.045
ER  - 

@article{
author = "Stojković, Dejan S. and Kovačević Grujičić, Nataša and Reis, Filipa S. and Davidović, Slobodan and Barros, Lillian and Popović, Jelena and Petrović, Isidora and Pavić, Aleksandar and Glamoclija, Jasmina and Cirić, Ana and Stevanović, Milena and Ferreira, Isabel C. F. R. and Soković, Marina",
year = "2017",
abstract = "Wild Meripilus giganteus Karst belongs to the order Polyporales, in which some members are known to possess a wide range of pharmacological properties. M. giganteus showed to be rich in carbohydrates (74.49 g/100 g) and proteins (15.94 g/100 g), presenting low fat content (1.51 g/100 g). Chemical composition was determined by using chromatographic techniques. Also, various bioactive compounds were detected including all four tocopherol isoforms with delta- and gamma-tocopherols being predominant (123.35 and 77.80 mu g/100 g, respectively); five organic acids (oxalic, malic, quinic, citric and fumaric acids) with predominant malic acid (3.17 g/100 g); and three phenolic acids and related compounds (p-hydroxybenzoic, p-coumaric and cinnamic acids; 1010, 2420 and 340 mu g/100 g, respectively). M. giganteus methanolic extract exhibited antioxidant activity tested by five different assays with the strongest potential in TBARS assay (EC50 0.31 mg/mL); and antimicrobial activities (MIC/MBC 0.0125-5 mg/mL; MIC/MFC 0.025-0.4 mg/mL). Furthermore, treatment of cervical carcinoma cell line (HeLa) led to reduction in cell's viability in MTT assay (lC(50) 0.41 mg/mL after 48 h), induced process of apoptosis and inhibited cell's migration in vitro. The analysed extract was not toxic for zebrafish embryos (at 0.5 mg/mL), indicating its biosafety and potential application as a dietary supplement in chemoprevention.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Lwt-Food Science and Technology",
title = "Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract",
pages = "462-454",
volume = "79",
doi = "10.1016/j.lwt.2017.01.045"
}

Stojković, D. S., Kovačević Grujičić, N., Reis, F. S., Davidović, S., Barros, L., Popović, J., Petrović, I., Pavić, A., Glamoclija, J., Cirić, A., Stevanović, M., Ferreira, I. C. F. R.,& Soković, M.. (2017). Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract. in Lwt-Food Science and Technology
Elsevier Science Bv, Amsterdam., 79, 454-462.
https://doi.org/10.1016/j.lwt.2017.01.045

Stojković DS, Kovačević Grujičić N, Reis FS, Davidović S, Barros L, Popović J, Petrović I, Pavić A, Glamoclija J, Cirić A, Stevanović M, Ferreira ICFR, Soković M. Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract. in Lwt-Food Science and Technology. 2017;79:454-462.
doi:10.1016/j.lwt.2017.01.045 .

Stojković, Dejan S., Kovačević Grujičić, Nataša, Reis, Filipa S., Davidović, Slobodan, Barros, Lillian, Popović, Jelena, Petrović, Isidora, Pavić, Aleksandar, Glamoclija, Jasmina, Cirić, Ana, Stevanović, Milena, Ferreira, Isabel C. F. R., Soković, Marina, "Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract" in Lwt-Food Science and Technology, 79 (2017):454-462,
https://doi.org/10.1016/j.lwt.2017.01.045 . .

TY  - JOUR
AU  - Vicentić, Jelena Marjanovic
AU  - Schwirtlich, Marija
AU  - Kovačević Grujičić, Nataša
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1007
AB  - Glioblastoma (GBM) is one of the most aggressive and deadly forms of cancer. Literature data reveals that all-trans retinoic acid (ATRA) has anticancer effects on different types of tumor cells. However, data about the effects of ATRA on glioblastoma cells are contradictory. In this study, we examined whether ATRA treatment affects features of human glioblastoma U251 cells. To that end, the cells were treated with different concentrations of ATRA. Results obtained by MTT and the crystal violet assays imply that ATRA affected the viability of U251 glioblastoma cells in a dose-and time-dependent manner. Fluorescence staining of microtubule cytoskeleton protein a-tubulin revealed that ATRA induced changes in cell morphology. Using semi-quantitative RT-PCR we found that the expression of SOX3 and GFAP genes, as markers of neural differentiation, was not changed upon ATRA treatment. Thus, the observed changes in cell morphology after ATRA treatment are not associated with neural differentiation of U251 glioblastoma cells. The scratch-wound healing assay revealed that ATRA changed the mode of U251 cell migration from collective to single cell motility. The cell-matrix adhesion assay demonstrated that the pharmacologically relevant concentration of ATRA lowered the cell-matrix adhesion capability of U251 cells. In conclusion, our results imply that further studies are needed before ATRA could be considered for the treatment of glioblastoma.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - All-trans retinoic acid influences viability, migration and adhesion of U251 glioblastoma cells
EP  - 706
IS  - 4
SP  - 699
VL  - 69
DO  - 10.2298/ABS170327016M
ER  - 

@article{
author = "Vicentić, Jelena Marjanovic and Schwirtlich, Marija and Kovačević Grujičić, Nataša and Stevanović, Milena and Drakulić, Danijela",
year = "2017",
abstract = "Glioblastoma (GBM) is one of the most aggressive and deadly forms of cancer. Literature data reveals that all-trans retinoic acid (ATRA) has anticancer effects on different types of tumor cells. However, data about the effects of ATRA on glioblastoma cells are contradictory. In this study, we examined whether ATRA treatment affects features of human glioblastoma U251 cells. To that end, the cells were treated with different concentrations of ATRA. Results obtained by MTT and the crystal violet assays imply that ATRA affected the viability of U251 glioblastoma cells in a dose-and time-dependent manner. Fluorescence staining of microtubule cytoskeleton protein a-tubulin revealed that ATRA induced changes in cell morphology. Using semi-quantitative RT-PCR we found that the expression of SOX3 and GFAP genes, as markers of neural differentiation, was not changed upon ATRA treatment. Thus, the observed changes in cell morphology after ATRA treatment are not associated with neural differentiation of U251 glioblastoma cells. The scratch-wound healing assay revealed that ATRA changed the mode of U251 cell migration from collective to single cell motility. The cell-matrix adhesion assay demonstrated that the pharmacologically relevant concentration of ATRA lowered the cell-matrix adhesion capability of U251 cells. In conclusion, our results imply that further studies are needed before ATRA could be considered for the treatment of glioblastoma.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "All-trans retinoic acid influences viability, migration and adhesion of U251 glioblastoma cells",
pages = "706-699",
number = "4",
volume = "69",
doi = "10.2298/ABS170327016M"
}

Vicentić, J. M., Schwirtlich, M., Kovačević Grujičić, N., Stevanović, M.,& Drakulić, D.. (2017). All-trans retinoic acid influences viability, migration and adhesion of U251 glioblastoma cells. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 69(4), 699-706.
https://doi.org/10.2298/ABS170327016M

Vicentić JM, Schwirtlich M, Kovačević Grujičić N, Stevanović M, Drakulić D. All-trans retinoic acid influences viability, migration and adhesion of U251 glioblastoma cells. in Archives of Biological Sciences. 2017;69(4):699-706.
doi:10.2298/ABS170327016M .

Vicentić, Jelena Marjanovic, Schwirtlich, Marija, Kovačević Grujičić, Nataša, Stevanović, Milena, Drakulić, Danijela, "All-trans retinoic acid influences viability, migration and adhesion of U251 glioblastoma cells" in Archives of Biological Sciences, 69, no. 4 (2017):699-706,
https://doi.org/10.2298/ABS170327016M . .

TY  - JOUR
AU  - Smiljković, Marija
AU  - Stanisavljević Ninković, Danijela
AU  - Stojković, Dejan
AU  - Petrović, Isidora
AU  - Vicentić, Jelena Marjanovic
AU  - Popović, Jelena
AU  - Grdadolnik, Simona Golic
AU  - Marković, Dejan
AU  - Sanković-Babić, Snežana
AU  - Glamoclija, Jasmina
AU  - Stevanović, Milena
AU  - Soković, Marina
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1014
AB  - Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin- 7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra-and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell's viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used.
PB  - EXCLI Journal Managing Office, Dortmund
T2  - EXCLI Journal
T1  - Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions
EP  - 807
SP  - 795
VL  - 16
DO  - 10.17179/excli2017-300
ER  - 

@article{
author = "Smiljković, Marija and Stanisavljević Ninković, Danijela and Stojković, Dejan and Petrović, Isidora and Vicentić, Jelena Marjanovic and Popović, Jelena and Grdadolnik, Simona Golic and Marković, Dejan and Sanković-Babić, Snežana and Glamoclija, Jasmina and Stevanović, Milena and Soković, Marina",
year = "2017",
abstract = "Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin- 7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra-and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell's viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used.",
publisher = "EXCLI Journal Managing Office, Dortmund",
journal = "EXCLI Journal",
title = "Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions",
pages = "807-795",
volume = "16",
doi = "10.17179/excli2017-300"
}

Smiljković, M., Stanisavljević Ninković, D., Stojković, D., Petrović, I., Vicentić, J. M., Popović, J., Grdadolnik, S. G., Marković, D., Sanković-Babić, S., Glamoclija, J., Stevanović, M.,& Soković, M.. (2017). Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions. in EXCLI Journal
EXCLI Journal Managing Office, Dortmund., 16, 795-807.
https://doi.org/10.17179/excli2017-300

Smiljković M, Stanisavljević Ninković D, Stojković D, Petrović I, Vicentić JM, Popović J, Grdadolnik SG, Marković D, Sanković-Babić S, Glamoclija J, Stevanović M, Soković M. Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions. in EXCLI Journal. 2017;16:795-807.
doi:10.17179/excli2017-300 .

Smiljković, Marija, Stanisavljević Ninković, Danijela, Stojković, Dejan, Petrović, Isidora, Vicentić, Jelena Marjanovic, Popović, Jelena, Grdadolnik, Simona Golic, Marković, Dejan, Sanković-Babić, Snežana, Glamoclija, Jasmina, Stevanović, Milena, Soković, Marina, "Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions" in EXCLI Journal, 16 (2017):795-807,
https://doi.org/10.17179/excli2017-300 . .

TY  - JOUR
AU  - Stanisavljević Ninković, Danijela
AU  - Petrović, Isidora
AU  - Vuković, Vladanka
AU  - Schwirtlich, Marija
AU  - Gredić, Marija
AU  - Stevanović, Milena
AU  - Popović, Jelena
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/996
AB  - SOX14 is a member of the SOX family of transcription factors mainly involved in the regulation of neural development. Recently, it became evident that SOX14 is one of four hyper-methylated genes in cervical carcinoma, considered as a tumor suppressor candidate in this type of malignancy. In this paper we elucidated the role of SOX14 in the regulation of malignant properties of cervical carcinoma cells in vitro. Functional analysis performed in HeLa cells revealed that SOX14 overexpression decreased viability and promoted apoptosis through altering the expression of apoptosis related genes. Our results demonstrated that overexpression of SOX14 initiated accumulation of p53, demonstrating potential cross-talk between SOX14 and the p53 signaling pathway. Further analysis unambiguously showed that SOX14 triggered posttranslational modification of p53 protein, as detected by the significantly increased level of phospho-p53 (Ser-15) in SOX14-overexpressing HeLa cells. Moreover, the obtained results revealed that SOX14 activated p53 protein, which was confirmed by elevated p21 Waf1/Cip1, a well known target gene of p53. This study advances our understanding about the role of SOX14 and might explain the molecular mechanism by which this transcription factor could exert tumor suppressor properties in cervical carcinoma.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line
IS  - 9
VL  - 12
DO  - 10.1371/journal.pone.0184686
ER  - 

@article{
author = "Stanisavljević Ninković, Danijela and Petrović, Isidora and Vuković, Vladanka and Schwirtlich, Marija and Gredić, Marija and Stevanović, Milena and Popović, Jelena",
year = "2017",
abstract = "SOX14 is a member of the SOX family of transcription factors mainly involved in the regulation of neural development. Recently, it became evident that SOX14 is one of four hyper-methylated genes in cervical carcinoma, considered as a tumor suppressor candidate in this type of malignancy. In this paper we elucidated the role of SOX14 in the regulation of malignant properties of cervical carcinoma cells in vitro. Functional analysis performed in HeLa cells revealed that SOX14 overexpression decreased viability and promoted apoptosis through altering the expression of apoptosis related genes. Our results demonstrated that overexpression of SOX14 initiated accumulation of p53, demonstrating potential cross-talk between SOX14 and the p53 signaling pathway. Further analysis unambiguously showed that SOX14 triggered posttranslational modification of p53 protein, as detected by the significantly increased level of phospho-p53 (Ser-15) in SOX14-overexpressing HeLa cells. Moreover, the obtained results revealed that SOX14 activated p53 protein, which was confirmed by elevated p21 Waf1/Cip1, a well known target gene of p53. This study advances our understanding about the role of SOX14 and might explain the molecular mechanism by which this transcription factor could exert tumor suppressor properties in cervical carcinoma.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line",
number = "9",
volume = "12",
doi = "10.1371/journal.pone.0184686"
}

Stanisavljević Ninković, D., Petrović, I., Vuković, V., Schwirtlich, M., Gredić, M., Stevanović, M.,& Popović, J.. (2017). SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line. in PLoS One
Public Library Science, San Francisco., 12(9).
https://doi.org/10.1371/journal.pone.0184686

Stanisavljević Ninković D, Petrović I, Vuković V, Schwirtlich M, Gredić M, Stevanović M, Popović J. SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line. in PLoS One. 2017;12(9).
doi:10.1371/journal.pone.0184686 .

Stanisavljević Ninković, Danijela, Petrović, Isidora, Vuković, Vladanka, Schwirtlich, Marija, Gredić, Marija, Stevanović, Milena, Popović, Jelena, "SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line" in PLoS One, 12, no. 9 (2017),
https://doi.org/10.1371/journal.pone.0184686 . .

TY  - JOUR
AU  - Topalović, Vladanka
AU  - Krstić, Aleksandar
AU  - Schwirtlich, Marija
AU  - Dolfini, Diletta
AU  - Mantovani, Roberto
AU  - Stevanović, Milena
AU  - Mojsin, Marija
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1005
AB  - Sox3/SOX3 is one of the earliest neural markers in vertebrates. Together with the Sox1/SOX1 and Sox2/SOX2 genes it is implicated in the regulation of stem cell identity. In the present study, we performed the first analysis of epigenetic mechanisms (DNA methylation and histone marks) involved in the regulation of the human SOX3 gene expression during RA-induced neural differentiation of NT2/D1 cells. We show that the promoter of the human SOX3 gene is extremely hypomethylated both in undifferentiated NT2/D1 cells and during the early phases of RA-induced neural differentiation. By employing chromatin immunopre-cipitation, we analyze several histone modifications across different regions of the SOX3 gene and their dynamics following initiation of differentiation. In the same timeframe we investigate profiles of selected histone marks on the promoters of human SOX1 and SOX2 genes. We demonstrate differences in histone signatures of SOX1, SOX2 and SOX3 genes. Considering the importance of SOXB1 genes in the process of neural differentiation, the present study contributes to a better understanding of epigenetic mechanisms implicated in the regulation of pluripotency maintenance and commitment towards the neural lineage.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells
IS  - 9
VL  - 12
DO  - 10.1371/journal.pone.0184099
ER  - 

@article{
author = "Topalović, Vladanka and Krstić, Aleksandar and Schwirtlich, Marija and Dolfini, Diletta and Mantovani, Roberto and Stevanović, Milena and Mojsin, Marija",
year = "2017",
abstract = "Sox3/SOX3 is one of the earliest neural markers in vertebrates. Together with the Sox1/SOX1 and Sox2/SOX2 genes it is implicated in the regulation of stem cell identity. In the present study, we performed the first analysis of epigenetic mechanisms (DNA methylation and histone marks) involved in the regulation of the human SOX3 gene expression during RA-induced neural differentiation of NT2/D1 cells. We show that the promoter of the human SOX3 gene is extremely hypomethylated both in undifferentiated NT2/D1 cells and during the early phases of RA-induced neural differentiation. By employing chromatin immunopre-cipitation, we analyze several histone modifications across different regions of the SOX3 gene and their dynamics following initiation of differentiation. In the same timeframe we investigate profiles of selected histone marks on the promoters of human SOX1 and SOX2 genes. We demonstrate differences in histone signatures of SOX1, SOX2 and SOX3 genes. Considering the importance of SOXB1 genes in the process of neural differentiation, the present study contributes to a better understanding of epigenetic mechanisms implicated in the regulation of pluripotency maintenance and commitment towards the neural lineage.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells",
number = "9",
volume = "12",
doi = "10.1371/journal.pone.0184099"
}

Topalović, V., Krstić, A., Schwirtlich, M., Dolfini, D., Mantovani, R., Stevanović, M.,& Mojsin, M.. (2017). Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells. in PLoS One
Public Library Science, San Francisco., 12(9).
https://doi.org/10.1371/journal.pone.0184099

Topalović V, Krstić A, Schwirtlich M, Dolfini D, Mantovani R, Stevanović M, Mojsin M. Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells. in PLoS One. 2017;12(9).
doi:10.1371/journal.pone.0184099 .

Topalović, Vladanka, Krstić, Aleksandar, Schwirtlich, Marija, Dolfini, Diletta, Mantovani, Roberto, Stevanović, Milena, Mojsin, Marija, "Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells" in PLoS One, 12, no. 9 (2017),
https://doi.org/10.1371/journal.pone.0184099 . .

TY  - JOUR
AU  - Cuturilo, Goran
AU  - Drakulić, Danijela
AU  - Jovanović, Ida
AU  - Ilić, Slobodan
AU  - Kalanj, Jasna
AU  - Vulicević, Irena
AU  - Raus, Misela
AU  - Skorić, Dejan
AU  - Mijović, Marija
AU  - Medjo, Biljana
AU  - Rsovac, Snežana
AU  - Stevanović, Milena
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1035
AB  - 22q11.2 microdeletion is the most common microdeletion in humans. The purpose of this study was to evaluate postoperative outcome in children with 22q11.2 microdeletion who had undergone complete surgical correction of a congenital heart defect. The study included 34 patients who underwent complete correction of conotruncal heart defects. Of these, 17 patients diagnosed with 22q11.2 microdeletion represent the investigated group. Another 17 patients without 22q11.2 microdeletion represent the control group. Investigated and control groups differ significantly for total length of stay in the hospital (average 37.35 and 14.12 days, respectively); length of postoperative stay in the intensive care unit (average 10.82 and 6.76 days, respectively); sepsis (eight and two patients, respectively); administration of antibiotics (15 and seven patients, respectively); duration of antibiotic therapy (average 17.65 and 14.59 days, respectively); occurrence of hypocalcemia (16 and 0 patients, respectively); and initiation of peroral nutrition during the postoperative course (average 10.29 and 3.88 days, respectively). No difference was found for duration of ventilatory support (average 6.12 and 4.24 days, respectively), administration of total parenteral nutrition, and postoperative mortality rate. The study results suggest that genotype of 22q11.2 microdeletion affects postoperative outcome after cardiac surgery. Possible targets for intervention in postoperative intensive care management are prevention and treatment of systemic infections, monitoring, and treatment of hypocalcemias, rational administration of antibiotics and careful planning of nutrition. Consequently, this could shorten patients' intensive care stay and overall duration of hospitalization.
PB  - Springer, New York
T2  - Pediatric Cardiology
T1  - The Impact of 22q11.2 Microdeletion on Cardiac Surgery Postoperative Outcome
EP  - 1685
IS  - 8
SP  - 1680
VL  - 38
DO  - 10.1007/s00246-017-1713-7
ER  - 

@article{
author = "Cuturilo, Goran and Drakulić, Danijela and Jovanović, Ida and Ilić, Slobodan and Kalanj, Jasna and Vulicević, Irena and Raus, Misela and Skorić, Dejan and Mijović, Marija and Medjo, Biljana and Rsovac, Snežana and Stevanović, Milena",
year = "2017",
abstract = "22q11.2 microdeletion is the most common microdeletion in humans. The purpose of this study was to evaluate postoperative outcome in children with 22q11.2 microdeletion who had undergone complete surgical correction of a congenital heart defect. The study included 34 patients who underwent complete correction of conotruncal heart defects. Of these, 17 patients diagnosed with 22q11.2 microdeletion represent the investigated group. Another 17 patients without 22q11.2 microdeletion represent the control group. Investigated and control groups differ significantly for total length of stay in the hospital (average 37.35 and 14.12 days, respectively); length of postoperative stay in the intensive care unit (average 10.82 and 6.76 days, respectively); sepsis (eight and two patients, respectively); administration of antibiotics (15 and seven patients, respectively); duration of antibiotic therapy (average 17.65 and 14.59 days, respectively); occurrence of hypocalcemia (16 and 0 patients, respectively); and initiation of peroral nutrition during the postoperative course (average 10.29 and 3.88 days, respectively). No difference was found for duration of ventilatory support (average 6.12 and 4.24 days, respectively), administration of total parenteral nutrition, and postoperative mortality rate. The study results suggest that genotype of 22q11.2 microdeletion affects postoperative outcome after cardiac surgery. Possible targets for intervention in postoperative intensive care management are prevention and treatment of systemic infections, monitoring, and treatment of hypocalcemias, rational administration of antibiotics and careful planning of nutrition. Consequently, this could shorten patients' intensive care stay and overall duration of hospitalization.",
publisher = "Springer, New York",
journal = "Pediatric Cardiology",
title = "The Impact of 22q11.2 Microdeletion on Cardiac Surgery Postoperative Outcome",
pages = "1685-1680",
number = "8",
volume = "38",
doi = "10.1007/s00246-017-1713-7"
}

Cuturilo, G., Drakulić, D., Jovanović, I., Ilić, S., Kalanj, J., Vulicević, I., Raus, M., Skorić, D., Mijović, M., Medjo, B., Rsovac, S.,& Stevanović, M.. (2017). The Impact of 22q11.2 Microdeletion on Cardiac Surgery Postoperative Outcome. in Pediatric Cardiology
Springer, New York., 38(8), 1680-1685.
https://doi.org/10.1007/s00246-017-1713-7

Cuturilo G, Drakulić D, Jovanović I, Ilić S, Kalanj J, Vulicević I, Raus M, Skorić D, Mijović M, Medjo B, Rsovac S, Stevanović M. The Impact of 22q11.2 Microdeletion on Cardiac Surgery Postoperative Outcome. in Pediatric Cardiology. 2017;38(8):1680-1685.
doi:10.1007/s00246-017-1713-7 .

Cuturilo, Goran, Drakulić, Danijela, Jovanović, Ida, Ilić, Slobodan, Kalanj, Jasna, Vulicević, Irena, Raus, Misela, Skorić, Dejan, Mijović, Marija, Medjo, Biljana, Rsovac, Snežana, Stevanović, Milena, "The Impact of 22q11.2 Microdeletion on Cardiac Surgery Postoperative Outcome" in Pediatric Cardiology, 38, no. 8 (2017):1680-1685,
https://doi.org/10.1007/s00246-017-1713-7 . .

TY  - JOUR
AU  - Topalović, Vladanka
AU  - Schwirtlich, Marija
AU  - Stevanović, Milena
AU  - Mojsin, Marija
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1077
AB  - Transcription factors OCT4 and NANOG are main constituents of a functional network that controls proliferation and pluripotency maintenance of stem cells as well as early lineage decisions. We investigated expression profiles of OCT4 and NANOG during the early phases of neural differentiation using NT2/D1 cells induced by retinoic acid as an in vitro model system of human neurogenesis. We demonstrated decrease in OCT4 and NANOG mRNA and protein levels following exposure to retinoic acid. Next, by employing chromatin immunoprecipitation, we investigated profiles of selected H3 and H2B histone marks deposited on the promoters of the OCT4 and NANOG genes. We found decline in H3K4me3, H2BK5ac, and H2BK120ac on both promoters, which paralleled the decrease in OCT4 and NANOG expression. Moreover, we found that the H2BK16ac mark is differentially enriched on these two promoters, pointing to differences in epigenetic regulation of OCT4 and NANOG gene expression. Finally, based on our data, we suggest that the early response of pluripotency genes OCT4 and NANOG to the differentiation-inducing stimuli is mediated by dynamic changes in chromatin marks, while DNA methylation is acquired in the later stages of neurogenesis.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Biochemistry-Moscow
T1  - Histone modifications on the promoters of human OCT4 and NANOG genes at the onset of neural differentiation of NT2/D1 cells
EP  - 722
IS  - 6
SP  - 715
VL  - 82
DO  - 10.1134/S0006297917060086
ER  - 

@article{
author = "Topalović, Vladanka and Schwirtlich, Marija and Stevanović, Milena and Mojsin, Marija",
year = "2017",
abstract = "Transcription factors OCT4 and NANOG are main constituents of a functional network that controls proliferation and pluripotency maintenance of stem cells as well as early lineage decisions. We investigated expression profiles of OCT4 and NANOG during the early phases of neural differentiation using NT2/D1 cells induced by retinoic acid as an in vitro model system of human neurogenesis. We demonstrated decrease in OCT4 and NANOG mRNA and protein levels following exposure to retinoic acid. Next, by employing chromatin immunoprecipitation, we investigated profiles of selected H3 and H2B histone marks deposited on the promoters of the OCT4 and NANOG genes. We found decline in H3K4me3, H2BK5ac, and H2BK120ac on both promoters, which paralleled the decrease in OCT4 and NANOG expression. Moreover, we found that the H2BK16ac mark is differentially enriched on these two promoters, pointing to differences in epigenetic regulation of OCT4 and NANOG gene expression. Finally, based on our data, we suggest that the early response of pluripotency genes OCT4 and NANOG to the differentiation-inducing stimuli is mediated by dynamic changes in chromatin marks, while DNA methylation is acquired in the later stages of neurogenesis.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Biochemistry-Moscow",
title = "Histone modifications on the promoters of human OCT4 and NANOG genes at the onset of neural differentiation of NT2/D1 cells",
pages = "722-715",
number = "6",
volume = "82",
doi = "10.1134/S0006297917060086"
}

Topalović, V., Schwirtlich, M., Stevanović, M.,& Mojsin, M.. (2017). Histone modifications on the promoters of human OCT4 and NANOG genes at the onset of neural differentiation of NT2/D1 cells. in Biochemistry-Moscow
Maik Nauka/Interperiodica/Springer, New York., 82(6), 715-722.
https://doi.org/10.1134/S0006297917060086

Topalović V, Schwirtlich M, Stevanović M, Mojsin M. Histone modifications on the promoters of human OCT4 and NANOG genes at the onset of neural differentiation of NT2/D1 cells. in Biochemistry-Moscow. 2017;82(6):715-722.
doi:10.1134/S0006297917060086 .

Topalović, Vladanka, Schwirtlich, Marija, Stevanović, Milena, Mojsin, Marija, "Histone modifications on the promoters of human OCT4 and NANOG genes at the onset of neural differentiation of NT2/D1 cells" in Biochemistry-Moscow, 82, no. 6 (2017):715-722,
https://doi.org/10.1134/S0006297917060086 . .

TY  - JOUR
AU  - Cuturilo, Goran
AU  - Drakulić, Danijela
AU  - Jovanović, Ida
AU  - Krstić, Aleksandar
AU  - Đukić, Milan
AU  - Skorić, Dejan
AU  - Mijović, Marija
AU  - Stefanović, Igor
AU  - Milivojević, Milena
AU  - Stevanović, Milena
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/961
AB  - Objective: The incidence of the 22q11.2 microdeletion among children who have at least two out of five major clinical criteria for 22q11.2 deletion syndrome. Design: Prospective study. Setting: University Children's Hospital in Belgrade, Serbia between 2005 and 2014. Participants: 57 patients with clinical characteristics of 22q11.2 deletion syndrome. Methods: Standard G-banding cytogenetic analysis was performed in all children, and the 22q11.2 genomic region was examined using fluorescence in situ hybridization (FISH). For patients with no deletion detected by FISH, multiplex ligation dependent probe amplification (MLPA) analysis was also done in order to detect cryptic deletions of this region and to analyze other genomic loci associated with phenotypes resembling the syndrome. A selected group of patients diagnosed to have 22q11.2 microdeletion by FISH underwent MLPA testing in order to characterize the size and position of deletion. Outcome Measure: The frequency of 22q11.2 microdeletion among children with at least two of the five major characteristics of 22q11.2 deletion syndrome (heart malformations, facial dysmorphism, T-cell immunodeficiency, palatal clefts and hypocalcemia/hypoparathyroid ism) Results: Typical 22q11.2 microdeletion was detected in 42.1% of patients; heart malformation were identified in all of them, facial dysmorphism in 79.2%, immunological problems in 63.6%, hypocalcemia in 62.5% and cleft palate in 8.3%. Conclusions: A higher detection rate compared to one-feature criterion is obtained when at least two major features of 22q11.2 deletion syndrome are taking into consideration. The criteria applied in this study could be considered by centers in low-income countries.
PB  - Springer India
T2  - Indian Pediatrics
T1  - Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia
EP  - 789
IS  - 9
SP  - 786
VL  - 53
DO  - 10.1007/s13312-016-0931-z
ER  - 

@article{
author = "Cuturilo, Goran and Drakulić, Danijela and Jovanović, Ida and Krstić, Aleksandar and Đukić, Milan and Skorić, Dejan and Mijović, Marija and Stefanović, Igor and Milivojević, Milena and Stevanović, Milena",
year = "2016",
abstract = "Objective: The incidence of the 22q11.2 microdeletion among children who have at least two out of five major clinical criteria for 22q11.2 deletion syndrome. Design: Prospective study. Setting: University Children's Hospital in Belgrade, Serbia between 2005 and 2014. Participants: 57 patients with clinical characteristics of 22q11.2 deletion syndrome. Methods: Standard G-banding cytogenetic analysis was performed in all children, and the 22q11.2 genomic region was examined using fluorescence in situ hybridization (FISH). For patients with no deletion detected by FISH, multiplex ligation dependent probe amplification (MLPA) analysis was also done in order to detect cryptic deletions of this region and to analyze other genomic loci associated with phenotypes resembling the syndrome. A selected group of patients diagnosed to have 22q11.2 microdeletion by FISH underwent MLPA testing in order to characterize the size and position of deletion. Outcome Measure: The frequency of 22q11.2 microdeletion among children with at least two of the five major characteristics of 22q11.2 deletion syndrome (heart malformations, facial dysmorphism, T-cell immunodeficiency, palatal clefts and hypocalcemia/hypoparathyroid ism) Results: Typical 22q11.2 microdeletion was detected in 42.1% of patients; heart malformation were identified in all of them, facial dysmorphism in 79.2%, immunological problems in 63.6%, hypocalcemia in 62.5% and cleft palate in 8.3%. Conclusions: A higher detection rate compared to one-feature criterion is obtained when at least two major features of 22q11.2 deletion syndrome are taking into consideration. The criteria applied in this study could be considered by centers in low-income countries.",
publisher = "Springer India",
journal = "Indian Pediatrics",
title = "Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia",
pages = "789-786",
number = "9",
volume = "53",
doi = "10.1007/s13312-016-0931-z"
}

Cuturilo, G., Drakulić, D., Jovanović, I., Krstić, A., Đukić, M., Skorić, D., Mijović, M., Stefanović, I., Milivojević, M.,& Stevanović, M.. (2016). Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia. in Indian Pediatrics
Springer India., 53(9), 786-789.
https://doi.org/10.1007/s13312-016-0931-z

Cuturilo G, Drakulić D, Jovanović I, Krstić A, Đukić M, Skorić D, Mijović M, Stefanović I, Milivojević M, Stevanović M. Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia. in Indian Pediatrics. 2016;53(9):786-789.
doi:10.1007/s13312-016-0931-z .

Cuturilo, Goran, Drakulić, Danijela, Jovanović, Ida, Krstić, Aleksandar, Đukić, Milan, Skorić, Dejan, Mijović, Marija, Stefanović, Igor, Milivojević, Milena, Stevanović, Milena, "Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia" in Indian Pediatrics, 53, no. 9 (2016):786-789,
https://doi.org/10.1007/s13312-016-0931-z . .

TY  - JOUR
AU  - Rakonjac, Marijana
AU  - Cuturilo, Goran
AU  - Stevanović, Milena
AU  - Jelicić, Ljiljana
AU  - Subotić, Misko
AU  - Jovanović, Ida
AU  - Drakulić, Danijela
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/939
AB  - Background: 22q11.2DS is the most common microdeletion syndrome in humans, usually associated with speech and language delay (SLD). Approximately 75% of children with 22q11.2 microdeletion have congenital heart malformations (CHM) which after infant open-heart surgery might lead to SLD. Aims: The purpose of this study was to determine whether factors associated with microdeletion contribute to SLD in children with 22q11.2DS. Methods and procedures: We compared speech and language abilities of two groups of school-aged children: those with 22q11.2 microdeletion (E1) and those with the phenotype resembling 22q11.2DS but without the microdeletion (E2). An age-matched group of typically developing children was also tested. Outcomes and results: The obtained results revealed that children from group E1 have lower level of speech and language abilities compared to children from group E2 and control group. Additionally, mild to moderate SLD was detected in children from group E2 compared to children from the control group. Conclusions and implications: The obtained results imply that both CHM after infant open-heart surgery and other factors associated with 22q11.2 microdeletion, contribute to SLD in patients with 22q11.2 microdeletion. Based on this, we could postulate that there is/are some potential candidate gene(s), located in the 22q11.2 region, whose function could be important for speech and language development.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Research in Developmental Disabilities
T1  - Differences in speech and language abilities between children with 22q11.2 deletion syndrome and children with phenotypic features of 22q11.2 deletion syndrome but without microdeletion
EP  - 329
SP  - 322
VL  - 55
DO  - 10.1016/j.ridd.2016.05.006
ER  - 

@article{
author = "Rakonjac, Marijana and Cuturilo, Goran and Stevanović, Milena and Jelicić, Ljiljana and Subotić, Misko and Jovanović, Ida and Drakulić, Danijela",
year = "2016",
abstract = "Background: 22q11.2DS is the most common microdeletion syndrome in humans, usually associated with speech and language delay (SLD). Approximately 75% of children with 22q11.2 microdeletion have congenital heart malformations (CHM) which after infant open-heart surgery might lead to SLD. Aims: The purpose of this study was to determine whether factors associated with microdeletion contribute to SLD in children with 22q11.2DS. Methods and procedures: We compared speech and language abilities of two groups of school-aged children: those with 22q11.2 microdeletion (E1) and those with the phenotype resembling 22q11.2DS but without the microdeletion (E2). An age-matched group of typically developing children was also tested. Outcomes and results: The obtained results revealed that children from group E1 have lower level of speech and language abilities compared to children from group E2 and control group. Additionally, mild to moderate SLD was detected in children from group E2 compared to children from the control group. Conclusions and implications: The obtained results imply that both CHM after infant open-heart surgery and other factors associated with 22q11.2 microdeletion, contribute to SLD in patients with 22q11.2 microdeletion. Based on this, we could postulate that there is/are some potential candidate gene(s), located in the 22q11.2 region, whose function could be important for speech and language development.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Research in Developmental Disabilities",
title = "Differences in speech and language abilities between children with 22q11.2 deletion syndrome and children with phenotypic features of 22q11.2 deletion syndrome but without microdeletion",
pages = "329-322",
volume = "55",
doi = "10.1016/j.ridd.2016.05.006"
}

Rakonjac, M., Cuturilo, G., Stevanović, M., Jelicić, L., Subotić, M., Jovanović, I.,& Drakulić, D.. (2016). Differences in speech and language abilities between children with 22q11.2 deletion syndrome and children with phenotypic features of 22q11.2 deletion syndrome but without microdeletion. in Research in Developmental Disabilities
Pergamon-Elsevier Science Ltd, Oxford., 55, 322-329.
https://doi.org/10.1016/j.ridd.2016.05.006

Rakonjac M, Cuturilo G, Stevanović M, Jelicić L, Subotić M, Jovanović I, Drakulić D. Differences in speech and language abilities between children with 22q11.2 deletion syndrome and children with phenotypic features of 22q11.2 deletion syndrome but without microdeletion. in Research in Developmental Disabilities. 2016;55:322-329.
doi:10.1016/j.ridd.2016.05.006 .

Rakonjac, Marijana, Cuturilo, Goran, Stevanović, Milena, Jelicić, Ljiljana, Subotić, Misko, Jovanović, Ida, Drakulić, Danijela, "Differences in speech and language abilities between children with 22q11.2 deletion syndrome and children with phenotypic features of 22q11.2 deletion syndrome but without microdeletion" in Research in Developmental Disabilities, 55 (2016):322-329,
https://doi.org/10.1016/j.ridd.2016.05.006 . .