TY  - JOUR
AU  - Milzarek, Tobias M.
AU  - Stevanović, Milena
AU  - Milivojević, Dušan
AU  - Vojnović, Sandra
AU  - Iliasov, Denis
AU  - Wolf, Diana
AU  - Mascher, Thorsten
AU  - Nikodinović-Runić, Jasmina
AU  - Gulder, Tobias A. M.
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2077
AB  - The ambigols are cyanobacterial natural products characterized by three polychlorinated aromatic building blocks connected by biaryl and biaryl ether bridges. All ambigols known to date possess promising biological activities. Most significantly, ambigol A was reported to have antibacterial activity against Gram-positive bacteria, such as Bacillus megaterium and B. subtilis. We established a diverse compound library for in-depth biological evaluation building on our previous bio- and total synthetic research on this natural product family. To explore the antimicrobial potential in detail and to determine initial structure–activity relationships of this product class, a large set of dimeric and trimeric compounds were screened against selected bacterial and Candida target strains. Our results reveal exceptional antibiotic activity of the ambigols, especially against challenging clinical isolates.
T2  - ACS Infectious Diseases
T2  - ACS Infectious DiseasesACS Infect. Dis.
T1  - Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs
DO  - 10.1021/acsinfecdis.3c00232
ER  - 

@article{
author = "Milzarek, Tobias M. and Stevanović, Milena and Milivojević, Dušan and Vojnović, Sandra and Iliasov, Denis and Wolf, Diana and Mascher, Thorsten and Nikodinović-Runić, Jasmina and Gulder, Tobias A. M.",
year = "2023",
abstract = "The ambigols are cyanobacterial natural products characterized by three polychlorinated aromatic building blocks connected by biaryl and biaryl ether bridges. All ambigols known to date possess promising biological activities. Most significantly, ambigol A was reported to have antibacterial activity against Gram-positive bacteria, such as Bacillus megaterium and B. subtilis. We established a diverse compound library for in-depth biological evaluation building on our previous bio- and total synthetic research on this natural product family. To explore the antimicrobial potential in detail and to determine initial structure–activity relationships of this product class, a large set of dimeric and trimeric compounds were screened against selected bacterial and Candida target strains. Our results reveal exceptional antibiotic activity of the ambigols, especially against challenging clinical isolates.",
journal = "ACS Infectious Diseases, ACS Infectious DiseasesACS Infect. Dis.",
title = "Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs",
doi = "10.1021/acsinfecdis.3c00232"
}

Milzarek, T. M., Stevanović, M., Milivojević, D., Vojnović, S., Iliasov, D., Wolf, D., Mascher, T., Nikodinović-Runić, J.,& Gulder, T. A. M.. (2023). Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs. in ACS Infectious Diseases.
https://doi.org/10.1021/acsinfecdis.3c00232

Milzarek TM, Stevanović M, Milivojević D, Vojnović S, Iliasov D, Wolf D, Mascher T, Nikodinović-Runić J, Gulder TAM. Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs. in ACS Infectious Diseases. 2023;.
doi:10.1021/acsinfecdis.3c00232 .

Milzarek, Tobias M., Stevanović, Milena, Milivojević, Dušan, Vojnović, Sandra, Iliasov, Denis, Wolf, Diana, Mascher, Thorsten, Nikodinović-Runić, Jasmina, Gulder, Tobias A. M., "Antibiotic potential of the Ambigol Cyanobacterial natural product class and simplified synthetic analogs" in ACS Infectious Diseases (2023),
https://doi.org/10.1021/acsinfecdis.3c00232 . .

TY  - JOUR
AU  - Pavić, Aleksandar
AU  - Ilić-Tomić, Tatjana
AU  - Glamoclija, Jasmina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1474
AB  - Severe drawbacks associated with the topical use of depigmenting agents in treatments of skin hyperigmentations impose a great demand for novel, effective, and safe melanogenesis inhibitors. Edible and medicinal mushrooms, known for numerous health-promoting properties, represent a rich reservoir of anti-melanogenic compounds, with the potential to be applied in preventing excessive skin pigmentation. Herein, using zebrafish (Danio rerio) as a preclinical animal model, we have demonstrated that ethanol extract of Laetiporus sulphureus (LSE) and Agaricus silvaticus (ASE) are not toxic at high doses up to 400-500 mu g/mL while effectively inhibit melanogenesis in a dose-dependent manner. At depigmenting doses, the explored extracts showed no adverse effects on zebrafish embryos melanocytes. Even more, they did not provoke inflammation or neutropenia when applied at the highest dose ensuring almost complete the cells depigmentation. Since LSE and ASE have demonstrated significantly higher the therapeutic potential than kojic acid and hydroquinone, two well-known depigmenting agents, overall results of this study strongly suggest that the explored mushrooms extracts could be used as efficient and safe topical agents in treatments of skin hyperpigmentation disorders.
PB  - MDPI, Basel
T2  - Journal of Fungi
T1  - Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model
IS  - 10
VL  - 7
DO  - 10.3390/jof7100834
ER  - 

@article{
author = "Pavić, Aleksandar and Ilić-Tomić, Tatjana and Glamoclija, Jasmina",
year = "2021",
abstract = "Severe drawbacks associated with the topical use of depigmenting agents in treatments of skin hyperigmentations impose a great demand for novel, effective, and safe melanogenesis inhibitors. Edible and medicinal mushrooms, known for numerous health-promoting properties, represent a rich reservoir of anti-melanogenic compounds, with the potential to be applied in preventing excessive skin pigmentation. Herein, using zebrafish (Danio rerio) as a preclinical animal model, we have demonstrated that ethanol extract of Laetiporus sulphureus (LSE) and Agaricus silvaticus (ASE) are not toxic at high doses up to 400-500 mu g/mL while effectively inhibit melanogenesis in a dose-dependent manner. At depigmenting doses, the explored extracts showed no adverse effects on zebrafish embryos melanocytes. Even more, they did not provoke inflammation or neutropenia when applied at the highest dose ensuring almost complete the cells depigmentation. Since LSE and ASE have demonstrated significantly higher the therapeutic potential than kojic acid and hydroquinone, two well-known depigmenting agents, overall results of this study strongly suggest that the explored mushrooms extracts could be used as efficient and safe topical agents in treatments of skin hyperpigmentation disorders.",
publisher = "MDPI, Basel",
journal = "Journal of Fungi",
title = "Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model",
number = "10",
volume = "7",
doi = "10.3390/jof7100834"
}

Pavić, A., Ilić-Tomić, T.,& Glamoclija, J.. (2021). Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model. in Journal of Fungi
MDPI, Basel., 7(10).
https://doi.org/10.3390/jof7100834

Pavić A, Ilić-Tomić T, Glamoclija J. Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model. in Journal of Fungi. 2021;7(10).
doi:10.3390/jof7100834 .

Pavić, Aleksandar, Ilić-Tomić, Tatjana, Glamoclija, Jasmina, "Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model" in Journal of Fungi, 7, no. 10 (2021),
https://doi.org/10.3390/jof7100834 . .

TY  - JOUR
AU  - Malagurski, Ivana
AU  - Frison, Ruggero
AU  - Maurya, Anjani K.
AU  - Neels, Antonia
AU  - Anđelković, Boban
AU  - Senthamaraikannan, Ramsankar
AU  - Babu, Ramesh
AU  - O'Connor, Kevin 
AU  - Witko, Tomasz
AU  - Solarz, Daria
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1481
AB  - New polyhydroxyoctanoate based composites with incorporated TiO2 microfibers were produced. The presence of the inorganic constituent influenced morphology, physical properties and functionality of the obtained biomaterials. The degree of PHO crystallinity decreased in the composites in a TiO2 concentration dependent manner. The composites were stiffer than the neat PHO, however they preserved their flexibility. Biocompatibility and cellular migration studies showed that composites support cell viability and migration. The obtained results suggest that PHO/TiO2 composites could be used as novel biomaterials with tunable properties for biomedical applications.
PB  - Elsevier, Amsterdam
T2  - Materials Letters
T1  - Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application
VL  - 285
DO  - 10.1016/j.matlet.2020.129100
ER  - 

@article{
author = "Malagurski, Ivana and Frison, Ruggero and Maurya, Anjani K. and Neels, Antonia and Anđelković, Boban and Senthamaraikannan, Ramsankar and Babu, Ramesh and O'Connor, Kevin  and Witko, Tomasz and Solarz, Daria and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "New polyhydroxyoctanoate based composites with incorporated TiO2 microfibers were produced. The presence of the inorganic constituent influenced morphology, physical properties and functionality of the obtained biomaterials. The degree of PHO crystallinity decreased in the composites in a TiO2 concentration dependent manner. The composites were stiffer than the neat PHO, however they preserved their flexibility. Biocompatibility and cellular migration studies showed that composites support cell viability and migration. The obtained results suggest that PHO/TiO2 composites could be used as novel biomaterials with tunable properties for biomedical applications.",
publisher = "Elsevier, Amsterdam",
journal = "Materials Letters",
title = "Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application",
volume = "285",
doi = "10.1016/j.matlet.2020.129100"
}

Malagurski, I., Frison, R., Maurya, A. K., Neels, A., Anđelković, B., Senthamaraikannan, R., Babu, R., O'Connor, K., Witko, T., Solarz, D.,& Nikodinović-Runić, J.. (2021). Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application. in Materials Letters
Elsevier, Amsterdam., 285.
https://doi.org/10.1016/j.matlet.2020.129100

Malagurski I, Frison R, Maurya AK, Neels A, Anđelković B, Senthamaraikannan R, Babu R, O'Connor K, Witko T, Solarz D, Nikodinović-Runić J. Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application. in Materials Letters. 2021;285.
doi:10.1016/j.matlet.2020.129100 .

Malagurski, Ivana, Frison, Ruggero, Maurya, Anjani K., Neels, Antonia, Anđelković, Boban, Senthamaraikannan, Ramsankar, Babu, Ramesh, O'Connor, Kevin , Witko, Tomasz, Solarz, Daria, Nikodinović-Runić, Jasmina, "Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application" in Materials Letters, 285 (2021),
https://doi.org/10.1016/j.matlet.2020.129100 . .

TY  - THES
AU  - Mandić, Mina
PY  - 2021
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=8478
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:24879/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=52766473
UR  - https://nardus.mpn.gov.rs/handle/123456789/18902
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/64
AB  - Bakterije roda Pseudomonas imaju sposobnost produkcije i razgradnje širokog spektra jedinjenja. Cilj ove doktorske teze bio je da se u kolekciji sojeva roda Pseudomonas identifikuju i rekombinantno eksprimiraju nove lakaze i lipaze, i evaluira njihov aplikativni potencijal. Sedam sojeva obuhvaćeno je analizom oksidativne i hidrolitičke aktivnosti prema pet različitih supstrata. Kod sojeva Pseudomonas putida F6, P. putida KT2440 i P. putida CA-3, utvrđeno je prisustvo gena koji kodiraju lakaze. Proteini McoCA3, McoKT, Cbp i CopA heterologo su eksprimirani u E. coli domaćinu, prečišćeni i okarakterisani. Pokazana je njihova aktivnost u širokom pH i temperaturnom opsegu kao i visoka termostabilnost. Ispitana je i sposobnost enzima da razgrade boje koje se koriste u tekstilnoj industriji, potencijalne zagađivače voda. Ćelijski ekstrakt koji je sadržao CopA enzim iz P. putida F6 pokazao je najznačajniju aktivnost razgradivši pet od sedam testiranih boja. Kod sojeva P. aeruginosa PAO1 i P. chlororaphis B-561, detektovana je lipolitička aktivnost i utvrđen potencijal za biorazgradnju polimera kao što su polihidroksialkanoati srednjeg lanca i polikaprolakton, a degradacioni potencijal soja B-561 potvrđen je i u modelu komposta. Homologo su eksprimirana tri proteina – LipA, PlcB i LipA, ali je zaključeno da nije došlo do njihove sekrecije. U ovom radu je pokazano da su Pseudomonas sojevi dobar izvor biotehnološki značajnih enzima lakaza i lipaza. Rekombinantna ekspresija lakaza dala je funkcionalne enzime pogodne za primenu u razgradnji sintetičkih boja iz otpadnih voda. Za primenu rekombinantnih lipaza u biorazgradnji polimernih materijala neophodna je optimizacija sekrecije, ali divlji sojevi koji eksprimiraju ove enzime su dovoljno dobri biokatalizatori za njihovu razgradnju.
AB  - Pseudomonas strains have the ability to produce and degrade a wide range of compounds. The main objective of this thesis was to identify, recombinantly express and characterize novel laccases and lipases from Pseudomonas spp. collection, and to evaluate their application potential. Seven strains were analyzed for oxidative and hydrolytic activity towards five different substrates. Genes encoding laccases were detected in P. putida F6, P. putida KT2440 and P. putida CA-3 strains. Proteins McoCA3, McoKT, Cbp and CopA were heterologously expressed in E. coli, purified and characterized, exhibiting broad temperature and pH range and high thermal stability. The ability of enzymes to degrade textile dyes was also examined. All enzymes proved to be very efficient in degradation of synthetic dyes, with the CopA enzyme from P. putida F6 showing the most significant activity, degrading five out of seven tested dyes. Lipolytic activity was detected in two strains: P. aeruginosa PAO1 and P. chlororaphis B-561. The potential for biodegradation of polymeric materials such as medium chain length polyhydroxyalkanoates and polycaprolactone was demonstrated, while degradation potential of B-561 was confirmed in the compost system as well. Three lipases were homologously expressed – LipA, PlcB and LipA, but they were not secreted. This study showed that Pseudomonas genus is a good source of biotechnologically relevant enzymes. Recombinant laccase expression yielded enzymes suitable for application in the degradation of persistent synthetic dyes from wastewaters. For the application of recombinant lipases in the biodegradation of polymers, optimization of the secretion is necessary, however wild type strains expressing these enzymes are good enough biocatalysts for biodegradation purpose.
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Biotehnološki značajni enzimi iz sojeva roda Pseudomonas: identifikacija i rekombinantna ekspresija lakaza i lipaza
T1  - Biotechnologically relevant enzymes in strains of Pseudomonas genus: Identification and recombinant expression of laccases and lipases
UR  - https://hdl.handle.net/21.15107/rcub_nardus_18902
ER  - 

@phdthesis{
author = "Mandić, Mina",
year = "2021",
abstract = "Bakterije roda Pseudomonas imaju sposobnost produkcije i razgradnje širokog spektra jedinjenja. Cilj ove doktorske teze bio je da se u kolekciji sojeva roda Pseudomonas identifikuju i rekombinantno eksprimiraju nove lakaze i lipaze, i evaluira njihov aplikativni potencijal. Sedam sojeva obuhvaćeno je analizom oksidativne i hidrolitičke aktivnosti prema pet različitih supstrata. Kod sojeva Pseudomonas putida F6, P. putida KT2440 i P. putida CA-3, utvrđeno je prisustvo gena koji kodiraju lakaze. Proteini McoCA3, McoKT, Cbp i CopA heterologo su eksprimirani u E. coli domaćinu, prečišćeni i okarakterisani. Pokazana je njihova aktivnost u širokom pH i temperaturnom opsegu kao i visoka termostabilnost. Ispitana je i sposobnost enzima da razgrade boje koje se koriste u tekstilnoj industriji, potencijalne zagađivače voda. Ćelijski ekstrakt koji je sadržao CopA enzim iz P. putida F6 pokazao je najznačajniju aktivnost razgradivši pet od sedam testiranih boja. Kod sojeva P. aeruginosa PAO1 i P. chlororaphis B-561, detektovana je lipolitička aktivnost i utvrđen potencijal za biorazgradnju polimera kao što su polihidroksialkanoati srednjeg lanca i polikaprolakton, a degradacioni potencijal soja B-561 potvrđen je i u modelu komposta. Homologo su eksprimirana tri proteina – LipA, PlcB i LipA, ali je zaključeno da nije došlo do njihove sekrecije. U ovom radu je pokazano da su Pseudomonas sojevi dobar izvor biotehnološki značajnih enzima lakaza i lipaza. Rekombinantna ekspresija lakaza dala je funkcionalne enzime pogodne za primenu u razgradnji sintetičkih boja iz otpadnih voda. Za primenu rekombinantnih lipaza u biorazgradnji polimernih materijala neophodna je optimizacija sekrecije, ali divlji sojevi koji eksprimiraju ove enzime su dovoljno dobri biokatalizatori za njihovu razgradnju., Pseudomonas strains have the ability to produce and degrade a wide range of compounds. The main objective of this thesis was to identify, recombinantly express and characterize novel laccases and lipases from Pseudomonas spp. collection, and to evaluate their application potential. Seven strains were analyzed for oxidative and hydrolytic activity towards five different substrates. Genes encoding laccases were detected in P. putida F6, P. putida KT2440 and P. putida CA-3 strains. Proteins McoCA3, McoKT, Cbp and CopA were heterologously expressed in E. coli, purified and characterized, exhibiting broad temperature and pH range and high thermal stability. The ability of enzymes to degrade textile dyes was also examined. All enzymes proved to be very efficient in degradation of synthetic dyes, with the CopA enzyme from P. putida F6 showing the most significant activity, degrading five out of seven tested dyes. Lipolytic activity was detected in two strains: P. aeruginosa PAO1 and P. chlororaphis B-561. The potential for biodegradation of polymeric materials such as medium chain length polyhydroxyalkanoates and polycaprolactone was demonstrated, while degradation potential of B-561 was confirmed in the compost system as well. Three lipases were homologously expressed – LipA, PlcB and LipA, but they were not secreted. This study showed that Pseudomonas genus is a good source of biotechnologically relevant enzymes. Recombinant laccase expression yielded enzymes suitable for application in the degradation of persistent synthetic dyes from wastewaters. For the application of recombinant lipases in the biodegradation of polymers, optimization of the secretion is necessary, however wild type strains expressing these enzymes are good enough biocatalysts for biodegradation purpose.",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Biotehnološki značajni enzimi iz sojeva roda Pseudomonas: identifikacija i rekombinantna ekspresija lakaza i lipaza, Biotechnologically relevant enzymes in strains of Pseudomonas genus: Identification and recombinant expression of laccases and lipases",
url = "https://hdl.handle.net/21.15107/rcub_nardus_18902"
}

Mandić, M.. (2021). Biotehnološki značajni enzimi iz sojeva roda Pseudomonas: identifikacija i rekombinantna ekspresija lakaza i lipaza. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_18902

Mandić M. Biotehnološki značajni enzimi iz sojeva roda Pseudomonas: identifikacija i rekombinantna ekspresija lakaza i lipaza. 2021;.
https://hdl.handle.net/21.15107/rcub_nardus_18902 .

Mandić, Mina, "Biotehnološki značajni enzimi iz sojeva roda Pseudomonas: identifikacija i rekombinantna ekspresija lakaza i lipaza" (2021),
https://hdl.handle.net/21.15107/rcub_nardus_18902 .

TY  - JOUR
AU  - Novakovi, Miroslav M.
AU  - Ilić-Tomić, Tatjana
AU  - Tešević, Vele
AU  - Simić, Katarina
AU  - Ivanović, Stefan
AU  - Simić, Stefan
AU  - Opsenica, Igor
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1310
AB  - TheTrametes versicolorlaccase catalyzed oxidation of chalcone butein afforded four dimers of aurone sulfuretin (i.e.two regioisomeric pairs of diasteromers,1-4) as the major products. The formation of the dimers was explained by a two step process involving the initial cyclization of butein into aurone sulfuretin, followed by the combination of two molecules of sulfuretin. The coupling process occurred between the 2,10-double bond of one molecule of sulfuretin and the (3 ',4 ') catechol group of the other to yield a dimeric structure. This was confirmed by the experiment involving the laccase catalyzed oxidation of sulfuretin yielding the same dimeric bisaurones. Compounds1,3and4, were isolated using semipreparative HPLC and characterized by the detailed analysis of the NMR, MS, IR, and UV-vis data. The structure of compound2, isolated as a mixture containingca.25% of compound1,was proposed by the comparison of(1)H NMR data to compound1and by using LC-ESIMS analysis. The starting chalcone butein and the products of the biocatalytic transformation, aurone sulfuretin and sulfuretin dimers1,3and4,were evaluated for their cytotoxic and antioxidative propertiesin vitrousing a healthy human fibroblast (MRC5) cell line. The biotransformation products showed lower cytotoxicity but higher antioxidative properties. TheC. coggygriabark methanol extract rich in butein and sulfuretin was also biotransformed by laccase. The transformed extract exhibited significantly improved antioxidative activities.
PB  - Royal Soc Chemistry, Cambridge
T2  - New Journal of Chemistry
T1  - Bisaurones - enzymatic production and biological evaluation
EP  - 9655
IS  - 23
SP  - 9647
VL  - 44
DO  - 10.1039/d0nj00758g
ER  - 

@article{
author = "Novakovi, Miroslav M. and Ilić-Tomić, Tatjana and Tešević, Vele and Simić, Katarina and Ivanović, Stefan and Simić, Stefan and Opsenica, Igor and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "TheTrametes versicolorlaccase catalyzed oxidation of chalcone butein afforded four dimers of aurone sulfuretin (i.e.two regioisomeric pairs of diasteromers,1-4) as the major products. The formation of the dimers was explained by a two step process involving the initial cyclization of butein into aurone sulfuretin, followed by the combination of two molecules of sulfuretin. The coupling process occurred between the 2,10-double bond of one molecule of sulfuretin and the (3 ',4 ') catechol group of the other to yield a dimeric structure. This was confirmed by the experiment involving the laccase catalyzed oxidation of sulfuretin yielding the same dimeric bisaurones. Compounds1,3and4, were isolated using semipreparative HPLC and characterized by the detailed analysis of the NMR, MS, IR, and UV-vis data. The structure of compound2, isolated as a mixture containingca.25% of compound1,was proposed by the comparison of(1)H NMR data to compound1and by using LC-ESIMS analysis. The starting chalcone butein and the products of the biocatalytic transformation, aurone sulfuretin and sulfuretin dimers1,3and4,were evaluated for their cytotoxic and antioxidative propertiesin vitrousing a healthy human fibroblast (MRC5) cell line. The biotransformation products showed lower cytotoxicity but higher antioxidative properties. TheC. coggygriabark methanol extract rich in butein and sulfuretin was also biotransformed by laccase. The transformed extract exhibited significantly improved antioxidative activities.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "New Journal of Chemistry",
title = "Bisaurones - enzymatic production and biological evaluation",
pages = "9655-9647",
number = "23",
volume = "44",
doi = "10.1039/d0nj00758g"
}

Novakovi, M. M., Ilić-Tomić, T., Tešević, V., Simić, K., Ivanović, S., Simić, S., Opsenica, I.,& Nikodinović-Runić, J.. (2020). Bisaurones - enzymatic production and biological evaluation. in New Journal of Chemistry
Royal Soc Chemistry, Cambridge., 44(23), 9647-9655.
https://doi.org/10.1039/d0nj00758g

Novakovi MM, Ilić-Tomić T, Tešević V, Simić K, Ivanović S, Simić S, Opsenica I, Nikodinović-Runić J. Bisaurones - enzymatic production and biological evaluation. in New Journal of Chemistry. 2020;44(23):9647-9655.
doi:10.1039/d0nj00758g .

Novakovi, Miroslav M., Ilić-Tomić, Tatjana, Tešević, Vele, Simić, Katarina, Ivanović, Stefan, Simić, Stefan, Opsenica, Igor, Nikodinović-Runić, Jasmina, "Bisaurones - enzymatic production and biological evaluation" in New Journal of Chemistry, 44, no. 23 (2020):9647-9655,
https://doi.org/10.1039/d0nj00758g . .

TY  - JOUR
AU  - Kurutos, Atanas
AU  - Ilić-Tomić, Tatjana
AU  - Kamounah, Fadhil S.
AU  - Vasilev, Aleksey A.
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1328
AB  - Cyanine based chemosensing platforms have successfully been employed over the past couple of decades in various fields of biomolecular sciences. Still a substantial number of recent advances and improvements on this class of compounds are reported in the art. This paper presents our latest work on the improved synthetic approach, study on photophysical properties, and biosensing applicability of monomethine cyanine dyes. The series of mono-, di- and tricationic dyes showed up to 5-fold enhanced resistance against photobleaching compared to the commercially available Thiazole Orange (TO). The title compounds were studied as potential molecular probes for the detection of deoxyribonucleic acid, demonstrating their capacity as excellent fluorescent labeling agents. Depending on the dye chemical structure, current Cl-TO compounds exhibit up to 834-fold enhanced fluorescence emission and form stable complexes with Calf Thymus-DNA. The calculated binding constants were found to be higher than several conventional fluorogenic dyes for nucleic acid detection. All studied derivatives appeared as less cytotoxic than the Thiazole Orange. IC50 concentrations in human fibro-blasts MRCS cell line were calculated up to 50 mu M for the synthesized Cl-TO dyes, and 0.5 mu M for the parental Thiazole Orange. Two of the dyes were found very competent in post-electrophoretic visualization of DNA. As demonstrated by the agarose gel electrophoresis, the staining efficiency and detection limits of the dyes were comparable to the widely used Ethidium Bromide. The tricationic dye revealed great potential for cell cycle analysis in G1, S and G2 phases. The chlorinated TO derivatives readily stain human cells in vivo, while they can effectively be applied for eukaryotic and microbial cell staining.
PB  - Elsevier Science Sa, Lausanne
T2  - Journal of Photochemistry and Photobiology A-Chemistry
T1  - Non-cytotoxic photostable monomethine cyanine platforms: Combined paradigm of nucleic acid staining and in vivo imaging
VL  - 397
DO  - 10.1016/j.jphotochem.2020.112598
ER  - 

@article{
author = "Kurutos, Atanas and Ilić-Tomić, Tatjana and Kamounah, Fadhil S. and Vasilev, Aleksey A. and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "Cyanine based chemosensing platforms have successfully been employed over the past couple of decades in various fields of biomolecular sciences. Still a substantial number of recent advances and improvements on this class of compounds are reported in the art. This paper presents our latest work on the improved synthetic approach, study on photophysical properties, and biosensing applicability of monomethine cyanine dyes. The series of mono-, di- and tricationic dyes showed up to 5-fold enhanced resistance against photobleaching compared to the commercially available Thiazole Orange (TO). The title compounds were studied as potential molecular probes for the detection of deoxyribonucleic acid, demonstrating their capacity as excellent fluorescent labeling agents. Depending on the dye chemical structure, current Cl-TO compounds exhibit up to 834-fold enhanced fluorescence emission and form stable complexes with Calf Thymus-DNA. The calculated binding constants were found to be higher than several conventional fluorogenic dyes for nucleic acid detection. All studied derivatives appeared as less cytotoxic than the Thiazole Orange. IC50 concentrations in human fibro-blasts MRCS cell line were calculated up to 50 mu M for the synthesized Cl-TO dyes, and 0.5 mu M for the parental Thiazole Orange. Two of the dyes were found very competent in post-electrophoretic visualization of DNA. As demonstrated by the agarose gel electrophoresis, the staining efficiency and detection limits of the dyes were comparable to the widely used Ethidium Bromide. The tricationic dye revealed great potential for cell cycle analysis in G1, S and G2 phases. The chlorinated TO derivatives readily stain human cells in vivo, while they can effectively be applied for eukaryotic and microbial cell staining.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Journal of Photochemistry and Photobiology A-Chemistry",
title = "Non-cytotoxic photostable monomethine cyanine platforms: Combined paradigm of nucleic acid staining and in vivo imaging",
volume = "397",
doi = "10.1016/j.jphotochem.2020.112598"
}

Kurutos, A., Ilić-Tomić, T., Kamounah, F. S., Vasilev, A. A.,& Nikodinović-Runić, J.. (2020). Non-cytotoxic photostable monomethine cyanine platforms: Combined paradigm of nucleic acid staining and in vivo imaging. in Journal of Photochemistry and Photobiology A-Chemistry
Elsevier Science Sa, Lausanne., 397.
https://doi.org/10.1016/j.jphotochem.2020.112598

Kurutos A, Ilić-Tomić T, Kamounah FS, Vasilev AA, Nikodinović-Runić J. Non-cytotoxic photostable monomethine cyanine platforms: Combined paradigm of nucleic acid staining and in vivo imaging. in Journal of Photochemistry and Photobiology A-Chemistry. 2020;397.
doi:10.1016/j.jphotochem.2020.112598 .

Kurutos, Atanas, Ilić-Tomić, Tatjana, Kamounah, Fadhil S., Vasilev, Aleksey A., Nikodinović-Runić, Jasmina, "Non-cytotoxic photostable monomethine cyanine platforms: Combined paradigm of nucleic acid staining and in vivo imaging" in Journal of Photochemistry and Photobiology A-Chemistry, 397 (2020),
https://doi.org/10.1016/j.jphotochem.2020.112598 . .

TY  - JOUR
AU  - Novaković, Miroslav
AU  - Simić, Stefan
AU  - Koracak, Ljiljana
AU  - Zlatović, Mario
AU  - Ilić-Tomić, Tatjana
AU  - Asakawa, Yoshinori
AU  - Nikodinović-Runić, Jasmina
AU  - Opsenica, Igor
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1367
AB  - Chemical and biocatalytic synthesis of seven previously undescribed marchantin A ester derivatives has been presented. Chemical synthesis afforded three peresterified bisbibenzyl products (TE1-TE3), while enzymatic method, using lipase, produced regioselective monoester derivatives (ME1-ME4). The antiproliferative activities of all prepared derivatives of marchantin A were tested on MRC-5 healthy human lung fibroblast, A549 human lung cancer, and MDA-MB-231 human breast cancer cell lines. All tested esters were less cytotoxic in comparison to marchantin A, but they also exhibited lower cytotoxicity against healthy cells. Monoesters displayed higher cytotoxic activities than the corresponding peresterified products, presumably due to the presence of free catechol group. Monohexanoyl ester ME3 displayed the same IC50 like marchantin A against MDA-MB-231 cells, but the selectivity was higher. In this way, regioselective enzymatic monoesterification enhanced selectivity of marchantin A. ME3 was also the most active among all derivatives against lung cancer cells A549 with the slightly lower activity and selectivity in comparison to marchantin A.
PB  - Elsevier, Amsterdam
T2  - Fitoterapia
T1  - Chemo- and biocatalytic esterification of marchantin A and cytotoxic activity of ester derivatives
VL  - 142
DO  - 10.1016/j.fitote.2020.104520
ER  - 

@article{
author = "Novaković, Miroslav and Simić, Stefan and Koracak, Ljiljana and Zlatović, Mario and Ilić-Tomić, Tatjana and Asakawa, Yoshinori and Nikodinović-Runić, Jasmina and Opsenica, Igor",
year = "2020",
abstract = "Chemical and biocatalytic synthesis of seven previously undescribed marchantin A ester derivatives has been presented. Chemical synthesis afforded three peresterified bisbibenzyl products (TE1-TE3), while enzymatic method, using lipase, produced regioselective monoester derivatives (ME1-ME4). The antiproliferative activities of all prepared derivatives of marchantin A were tested on MRC-5 healthy human lung fibroblast, A549 human lung cancer, and MDA-MB-231 human breast cancer cell lines. All tested esters were less cytotoxic in comparison to marchantin A, but they also exhibited lower cytotoxicity against healthy cells. Monoesters displayed higher cytotoxic activities than the corresponding peresterified products, presumably due to the presence of free catechol group. Monohexanoyl ester ME3 displayed the same IC50 like marchantin A against MDA-MB-231 cells, but the selectivity was higher. In this way, regioselective enzymatic monoesterification enhanced selectivity of marchantin A. ME3 was also the most active among all derivatives against lung cancer cells A549 with the slightly lower activity and selectivity in comparison to marchantin A.",
publisher = "Elsevier, Amsterdam",
journal = "Fitoterapia",
title = "Chemo- and biocatalytic esterification of marchantin A and cytotoxic activity of ester derivatives",
volume = "142",
doi = "10.1016/j.fitote.2020.104520"
}

Novaković, M., Simić, S., Koracak, L., Zlatović, M., Ilić-Tomić, T., Asakawa, Y., Nikodinović-Runić, J.,& Opsenica, I.. (2020). Chemo- and biocatalytic esterification of marchantin A and cytotoxic activity of ester derivatives. in Fitoterapia
Elsevier, Amsterdam., 142.
https://doi.org/10.1016/j.fitote.2020.104520

Novaković M, Simić S, Koracak L, Zlatović M, Ilić-Tomić T, Asakawa Y, Nikodinović-Runić J, Opsenica I. Chemo- and biocatalytic esterification of marchantin A and cytotoxic activity of ester derivatives. in Fitoterapia. 2020;142.
doi:10.1016/j.fitote.2020.104520 .

Novaković, Miroslav, Simić, Stefan, Koracak, Ljiljana, Zlatović, Mario, Ilić-Tomić, Tatjana, Asakawa, Yoshinori, Nikodinović-Runić, Jasmina, Opsenica, Igor, "Chemo- and biocatalytic esterification of marchantin A and cytotoxic activity of ester derivatives" in Fitoterapia, 142 (2020),
https://doi.org/10.1016/j.fitote.2020.104520 . .

TY  - JOUR
AU  - Petrović, Jovana
AU  - Glamoclija, Jasmina
AU  - Ilić-Tomić, Tatjana
AU  - Soković, Marina
AU  - Robajac, Dragana
AU  - Nedić, Olgica
AU  - Pavić, Aleksandar
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1383
AB  - In spite of extensive usage of Laetiporus sulphureus (sulphur polypore) in traditional European and Asian ethnomedicine for centuries, its anticancer therapeutic potential and toxicity profile remained explored in animal models. Herein, using zebrafish (Danio rerio), as a preclinical animal model, we demonstrated that L sulphureus lectin (LSL) and ethanol extract (LSE) are non-toxic at high doses up to 400-500 mu g/mL, while they effectively inhibited angiogenesis and cancer development at much lower doses. Lectin showed 74-fold higher antiangiogenic potency than the extract, and even 378-fold higher therapeutic potential than sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of clinical relevance. Using wound healing and MTT assays, we proved LSL's strong anti-migratory effect and selective endothelial cytotoxidty in relation to lung fibro-blasts. In addition, employing the zebrafish xenograft models, we demonstrated that LSL almost completely reduced growth, neovascularization and metastasis of human colorectal carcinoma and mouse melanoma. Even more, LSL exerted 8-fold higher potency towards colorectal carcinoma than melanoma, showing markedly higher activity than cisplatin, while LSE failed to express any anticancer activity. Accompanied with non-toxic response, including neutropenia and inflammation, the results of this study strongly imply that LSL could be used as safe adjuvant in chemotherapy against colorectal carcinoma and melanoma.
PB  - Elsevier, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma
EP  - 139
SP  - 129
VL  - 148
DO  - 10.1016/j.ijbiomac.2020.01.033
ER  - 

@article{
author = "Petrović, Jovana and Glamoclija, Jasmina and Ilić-Tomić, Tatjana and Soković, Marina and Robajac, Dragana and Nedić, Olgica and Pavić, Aleksandar",
year = "2020",
abstract = "In spite of extensive usage of Laetiporus sulphureus (sulphur polypore) in traditional European and Asian ethnomedicine for centuries, its anticancer therapeutic potential and toxicity profile remained explored in animal models. Herein, using zebrafish (Danio rerio), as a preclinical animal model, we demonstrated that L sulphureus lectin (LSL) and ethanol extract (LSE) are non-toxic at high doses up to 400-500 mu g/mL, while they effectively inhibited angiogenesis and cancer development at much lower doses. Lectin showed 74-fold higher antiangiogenic potency than the extract, and even 378-fold higher therapeutic potential than sunitinib-malate, cardiotoxic and myelosupressive anti-angiogenic drug of clinical relevance. Using wound healing and MTT assays, we proved LSL's strong anti-migratory effect and selective endothelial cytotoxidty in relation to lung fibro-blasts. In addition, employing the zebrafish xenograft models, we demonstrated that LSL almost completely reduced growth, neovascularization and metastasis of human colorectal carcinoma and mouse melanoma. Even more, LSL exerted 8-fold higher potency towards colorectal carcinoma than melanoma, showing markedly higher activity than cisplatin, while LSE failed to express any anticancer activity. Accompanied with non-toxic response, including neutropenia and inflammation, the results of this study strongly imply that LSL could be used as safe adjuvant in chemotherapy against colorectal carcinoma and melanoma.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma",
pages = "139-129",
volume = "148",
doi = "10.1016/j.ijbiomac.2020.01.033"
}

Petrović, J., Glamoclija, J., Ilić-Tomić, T., Soković, M., Robajac, D., Nedić, O.,& Pavić, A.. (2020). Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma. in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 148, 129-139.
https://doi.org/10.1016/j.ijbiomac.2020.01.033

Petrović J, Glamoclija J, Ilić-Tomić T, Soković M, Robajac D, Nedić O, Pavić A. Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma. in International Journal of Biological Macromolecules. 2020;148:129-139.
doi:10.1016/j.ijbiomac.2020.01.033 .

Petrović, Jovana, Glamoclija, Jasmina, Ilić-Tomić, Tatjana, Soković, Marina, Robajac, Dragana, Nedić, Olgica, Pavić, Aleksandar, "Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma" in International Journal of Biological Macromolecules, 148 (2020):129-139,
https://doi.org/10.1016/j.ijbiomac.2020.01.033 . .

TY  - JOUR
AU  - Novaković, Miroslav
AU  - Simić, Stefan
AU  - Koracak, Ljiljana
AU  - Zlatović, Mario
AU  - Ilić-Tomić, Tatjana
AU  - Asakawa, Yoshinori
AU  - Nikodinović-Runić, Jasmina
AU  - Opsenica, Igor
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1755
AB  - Chemical and biocatalytic synthesis of seven previously undescribed marchantin A ester derivatives has been presented. Chemical synthesis afforded three peresterified bisbibenzyl products (TE1-TE3), while enzymatic method, using lipase, produced regioselective monoester derivatives (ME1-ME4). The antiproliferative activities of all prepared derivatives of marchantin A were tested on MRC-5 healthy human lung fibroblast, A549 human lung cancer, and MDA-MB-231 human breast cancer cell lines. All tested esters were less cytotoxic in comparison to marchantin A, but they also exhibited lower cytotoxicity against healthy cells. Monoesters displayed higher cytotoxic activities than the corresponding peresterified products, presumably due to the presence of free catechol group. Monohexanoyl ester ME3 displayed the same IC50 like marchantin A against MDA-MB-231 cells, but the selectivity was higher. In this way, regioselective enzymatic monoesterification enhanced selectivity of marchantin A. ME3 was also the most active among all derivatives against lung cancer cells A549 with the slightly lower activity and selectivity in comparison to marchantin A.
PB  - Elsevier, Amsterdam
T2  - Fitoterapia
T1  - Chemo- and biocatalytic esterification of marchantin A and cytotoxic activity of ester derivatives
VL  - 142
DO  - 10.1016/j.fitote.2020.104520
ER  - 

@article{
author = "Novaković, Miroslav and Simić, Stefan and Koracak, Ljiljana and Zlatović, Mario and Ilić-Tomić, Tatjana and Asakawa, Yoshinori and Nikodinović-Runić, Jasmina and Opsenica, Igor",
year = "2020",
abstract = "Chemical and biocatalytic synthesis of seven previously undescribed marchantin A ester derivatives has been presented. Chemical synthesis afforded three peresterified bisbibenzyl products (TE1-TE3), while enzymatic method, using lipase, produced regioselective monoester derivatives (ME1-ME4). The antiproliferative activities of all prepared derivatives of marchantin A were tested on MRC-5 healthy human lung fibroblast, A549 human lung cancer, and MDA-MB-231 human breast cancer cell lines. All tested esters were less cytotoxic in comparison to marchantin A, but they also exhibited lower cytotoxicity against healthy cells. Monoesters displayed higher cytotoxic activities than the corresponding peresterified products, presumably due to the presence of free catechol group. Monohexanoyl ester ME3 displayed the same IC50 like marchantin A against MDA-MB-231 cells, but the selectivity was higher. In this way, regioselective enzymatic monoesterification enhanced selectivity of marchantin A. ME3 was also the most active among all derivatives against lung cancer cells A549 with the slightly lower activity and selectivity in comparison to marchantin A.",
publisher = "Elsevier, Amsterdam",
journal = "Fitoterapia",
title = "Chemo- and biocatalytic esterification of marchantin A and cytotoxic activity of ester derivatives",
volume = "142",
doi = "10.1016/j.fitote.2020.104520"
}

Novaković, M., Simić, S., Koracak, L., Zlatović, M., Ilić-Tomić, T., Asakawa, Y., Nikodinović-Runić, J.,& Opsenica, I.. (2020). Chemo- and biocatalytic esterification of marchantin A and cytotoxic activity of ester derivatives. in Fitoterapia
Elsevier, Amsterdam., 142.
https://doi.org/10.1016/j.fitote.2020.104520

Novaković M, Simić S, Koracak L, Zlatović M, Ilić-Tomić T, Asakawa Y, Nikodinović-Runić J, Opsenica I. Chemo- and biocatalytic esterification of marchantin A and cytotoxic activity of ester derivatives. in Fitoterapia. 2020;142.
doi:10.1016/j.fitote.2020.104520 .

Novaković, Miroslav, Simić, Stefan, Koracak, Ljiljana, Zlatović, Mario, Ilić-Tomić, Tatjana, Asakawa, Yoshinori, Nikodinović-Runić, Jasmina, Opsenica, Igor, "Chemo- and biocatalytic esterification of marchantin A and cytotoxic activity of ester derivatives" in Fitoterapia, 142 (2020),
https://doi.org/10.1016/j.fitote.2020.104520 . .

TY  - JOUR
AU  - Ilić-Tomić, Tatjana
AU  - Tešević, Vele
AU  - Simić, Katarina
AU  - Ivanović, Stefan
AU  - Simić, Stefan
AU  - Opsenica, Igor
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1761
AB  - TheTrametes versicolorlaccase catalyzed oxidation of chalcone butein afforded four dimers of aurone sulfuretin (i.e.two regioisomeric pairs of diasteromers,1-4) as the major products. The formation of the dimers was explained by a two step process involving the initial cyclization of butein into aurone sulfuretin, followed by the combination of two molecules of sulfuretin. The coupling process occurred between the 2,10-double bond of one molecule of sulfuretin and the (3 ',4 ') catechol group of the other to yield a dimeric structure. This was confirmed by the experiment involving the laccase catalyzed oxidation of sulfuretin yielding the same dimeric bisaurones. Compounds1,3and4, were isolated using semipreparative HPLC and characterized by the detailed analysis of the NMR, MS, IR, and UV-vis data. The structure of compound2, isolated as a mixture containingca.25% of compound1,was proposed by the comparison of(1)H NMR data to compound1and by using LC-ESIMS analysis. The starting chalcone butein and the products of the biocatalytic transformation, aurone sulfuretin and sulfuretin dimers1,3and4,were evaluated for their cytotoxic and antioxidative propertiesin vitrousing a healthy human fibroblast (MRC5) cell line. The biotransformation products showed lower cytotoxicity but higher antioxidative properties. TheC. coggygriabark methanol extract rich in butein and sulfuretin was also biotransformed by laccase. The transformed extract exhibited significantly improved antioxidative activities.
PB  - Royal Soc Chemistry, Cambridge
T2  - New Journal of Chemistry
T1  - Bisaurones - enzymatic production and biological evaluation
EP  - 9655
IS  - 23
SP  - 9647
VL  - 44
DO  - 10.1039/d0nj00758g
ER  - 

@article{
author = "Ilić-Tomić, Tatjana and Tešević, Vele and Simić, Katarina and Ivanović, Stefan and Simić, Stefan and Opsenica, Igor and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "TheTrametes versicolorlaccase catalyzed oxidation of chalcone butein afforded four dimers of aurone sulfuretin (i.e.two regioisomeric pairs of diasteromers,1-4) as the major products. The formation of the dimers was explained by a two step process involving the initial cyclization of butein into aurone sulfuretin, followed by the combination of two molecules of sulfuretin. The coupling process occurred between the 2,10-double bond of one molecule of sulfuretin and the (3 ',4 ') catechol group of the other to yield a dimeric structure. This was confirmed by the experiment involving the laccase catalyzed oxidation of sulfuretin yielding the same dimeric bisaurones. Compounds1,3and4, were isolated using semipreparative HPLC and characterized by the detailed analysis of the NMR, MS, IR, and UV-vis data. The structure of compound2, isolated as a mixture containingca.25% of compound1,was proposed by the comparison of(1)H NMR data to compound1and by using LC-ESIMS analysis. The starting chalcone butein and the products of the biocatalytic transformation, aurone sulfuretin and sulfuretin dimers1,3and4,were evaluated for their cytotoxic and antioxidative propertiesin vitrousing a healthy human fibroblast (MRC5) cell line. The biotransformation products showed lower cytotoxicity but higher antioxidative properties. TheC. coggygriabark methanol extract rich in butein and sulfuretin was also biotransformed by laccase. The transformed extract exhibited significantly improved antioxidative activities.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "New Journal of Chemistry",
title = "Bisaurones - enzymatic production and biological evaluation",
pages = "9655-9647",
number = "23",
volume = "44",
doi = "10.1039/d0nj00758g"
}

Ilić-Tomić, T., Tešević, V., Simić, K., Ivanović, S., Simić, S., Opsenica, I.,& Nikodinović-Runić, J.. (2020). Bisaurones - enzymatic production and biological evaluation. in New Journal of Chemistry
Royal Soc Chemistry, Cambridge., 44(23), 9647-9655.
https://doi.org/10.1039/d0nj00758g

Ilić-Tomić T, Tešević V, Simić K, Ivanović S, Simić S, Opsenica I, Nikodinović-Runić J. Bisaurones - enzymatic production and biological evaluation. in New Journal of Chemistry. 2020;44(23):9647-9655.
doi:10.1039/d0nj00758g .

Ilić-Tomić, Tatjana, Tešević, Vele, Simić, Katarina, Ivanović, Stefan, Simić, Stefan, Opsenica, Igor, Nikodinović-Runić, Jasmina, "Bisaurones - enzymatic production and biological evaluation" in New Journal of Chemistry, 44, no. 23 (2020):9647-9655,
https://doi.org/10.1039/d0nj00758g . .

TY  - CHAP
AU  - Šenerović, Lidija
AU  - Opsenica, Dejan
AU  - Morić, Ivana
AU  - Aleksić, Ivana
AU  - Spasić, M.
AU  - Vasiljević, Branka
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1348
AB  - Infective diseases have become health threat of a global proportion due to appearance and spread of microorganisms resistant to majority of therapeutics currently used for their treatment. Therefore, there is a constant need for development of new antimicrobial agents, as well as novel therapeutic strategies. Quinolines and quinolones, isolated from plants, animals, and microorganisms, have demonstrated numerous biological activities such as antimicrobial, insecticidal, anti-inflammatory, antiplatelet, and antitumor. For more than two centuries quinoline/quinolone moiety has been used as a scaffold for drug development and even today it represents an inexhaustible inspiration for design and development of novel semi-synthetic or synthetic agents exhibiting broad spectrum of bioactivities. The structural diversity of synthetized compounds provides high and selective activity attained through different mechanisms of action, as well as low toxicity on human cells. This review describes quinoline and quinolone derivatives with antibacterial, antifungal, anti-virulent, antiviral, and anti-parasitic activities with the focus on the last 10 years literature.
PB  - Springer
T2  - Biophysics of Infection
T1  - Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents
EP  - 69
SP  - 37
VL  - 1282
DO  - 10.1007/5584_2019_428
ER  - 

@inbook{
author = "Šenerović, Lidija and Opsenica, Dejan and Morić, Ivana and Aleksić, Ivana and Spasić, M. and Vasiljević, Branka",
year = "2020",
abstract = "Infective diseases have become health threat of a global proportion due to appearance and spread of microorganisms resistant to majority of therapeutics currently used for their treatment. Therefore, there is a constant need for development of new antimicrobial agents, as well as novel therapeutic strategies. Quinolines and quinolones, isolated from plants, animals, and microorganisms, have demonstrated numerous biological activities such as antimicrobial, insecticidal, anti-inflammatory, antiplatelet, and antitumor. For more than two centuries quinoline/quinolone moiety has been used as a scaffold for drug development and even today it represents an inexhaustible inspiration for design and development of novel semi-synthetic or synthetic agents exhibiting broad spectrum of bioactivities. The structural diversity of synthetized compounds provides high and selective activity attained through different mechanisms of action, as well as low toxicity on human cells. This review describes quinoline and quinolone derivatives with antibacterial, antifungal, anti-virulent, antiviral, and anti-parasitic activities with the focus on the last 10 years literature.",
publisher = "Springer",
journal = "Biophysics of Infection",
booktitle = "Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents",
pages = "69-37",
volume = "1282",
doi = "10.1007/5584_2019_428"
}

Šenerović, L., Opsenica, D., Morić, I., Aleksić, I., Spasić, M.,& Vasiljević, B.. (2020). Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents. in Biophysics of Infection
Springer., 1282, 37-69.
https://doi.org/10.1007/5584_2019_428

Šenerović L, Opsenica D, Morić I, Aleksić I, Spasić M, Vasiljević B. Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents. in Biophysics of Infection. 2020;1282:37-69.
doi:10.1007/5584_2019_428 .

Šenerović, Lidija, Opsenica, Dejan, Morić, Ivana, Aleksić, Ivana, Spasić, M., Vasiljević, Branka, "Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents" in Biophysics of Infection, 1282 (2020):37-69,
https://doi.org/10.1007/5584_2019_428 . .

TY  - JOUR
AU  - Durić, Sonja
AU  - Vojnović, Sandra
AU  - Pavić, Aleksandar
AU  - Mojicević, Marija
AU  - Wadepohl, Hubert
AU  - Savić, Nada D.
AU  - Popsavin, Mirjana
AU  - Nikodinović-Runić, Jasmina
AU  - Djuran, Milos 
AU  - Glišić, Biljana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1377
AB  - New polynuclear silver(I) complexes with 1,5-naphthyridine (1,5-naph), [Ag(NO3)(1,5-naph)](n) (Ag1), [Ag (CF3COO)(1,5-naph)]n (Ag2) and [Ag(CF3SO3)(1,5-naph)](n) (Ag3) were synthesized by the reaction of the corresponding silver(I) salt and 1,5-naph in ethanol at room temperature. These complexes were characterized by NMR, IR and UV Vis spectroscopy, while their crystal structures were determined by single-crystal X-ray diffraction analysis. In all these complexes, 1,5-naph acts as a bridging ligand between two Ag(I) ions, while the remaining coordination sites are occupied by oxygen atom(s) of the corresponding anion. The antimicrobial efficiency of these silver(I) complexes was evaluated against the broad panel of Gram-positive and Gram-negative bacteria and fungi. The complexes showed good to moderate antibacterial activity with the minimal inhibitory concentration (MIC) values being in the range 2.5-100 mu g/mL (6.5-333.3 mu M), while their antifungal activity against the investigated Candida spp. was significantly higher (MIC = 0.78-6.25 mu g/mL; 2.6-20.8 mu M). Moreover, complexes Ag1 and Ag2 effectively inhibited C. albicans biofilms formation, while Ag1 was also shown to inhibit the formation of mixed C. albicans/Pseudomonas aeruginosa biofilms. Toxicological evaluations on zebrafish (Dario rerio) embryos revealed that all silver(I) complexes could be applied as antifungal agents, whereas Ag3 had the best therapeutic potential showing both the lowest MIC values against the tested Candida strains and the non-toxic in vivo response in the zebrafish embryos at these doses.
PB  - Elsevier Science Inc, New York
T2  - Journal of Inorganic Biochemistry
T1  - New polynuclear 1,5-naphthyridine-silver(I) complexes as potential antimicrobial agents: The key role of the nature of donor coordinated to the metal center
VL  - 203
DO  - 10.1016/j.jinorgbio.2019.110872
ER  - 

@article{
author = "Durić, Sonja and Vojnović, Sandra and Pavić, Aleksandar and Mojicević, Marija and Wadepohl, Hubert and Savić, Nada D. and Popsavin, Mirjana and Nikodinović-Runić, Jasmina and Djuran, Milos  and Glišić, Biljana",
year = "2020",
abstract = "New polynuclear silver(I) complexes with 1,5-naphthyridine (1,5-naph), [Ag(NO3)(1,5-naph)](n) (Ag1), [Ag (CF3COO)(1,5-naph)]n (Ag2) and [Ag(CF3SO3)(1,5-naph)](n) (Ag3) were synthesized by the reaction of the corresponding silver(I) salt and 1,5-naph in ethanol at room temperature. These complexes were characterized by NMR, IR and UV Vis spectroscopy, while their crystal structures were determined by single-crystal X-ray diffraction analysis. In all these complexes, 1,5-naph acts as a bridging ligand between two Ag(I) ions, while the remaining coordination sites are occupied by oxygen atom(s) of the corresponding anion. The antimicrobial efficiency of these silver(I) complexes was evaluated against the broad panel of Gram-positive and Gram-negative bacteria and fungi. The complexes showed good to moderate antibacterial activity with the minimal inhibitory concentration (MIC) values being in the range 2.5-100 mu g/mL (6.5-333.3 mu M), while their antifungal activity against the investigated Candida spp. was significantly higher (MIC = 0.78-6.25 mu g/mL; 2.6-20.8 mu M). Moreover, complexes Ag1 and Ag2 effectively inhibited C. albicans biofilms formation, while Ag1 was also shown to inhibit the formation of mixed C. albicans/Pseudomonas aeruginosa biofilms. Toxicological evaluations on zebrafish (Dario rerio) embryos revealed that all silver(I) complexes could be applied as antifungal agents, whereas Ag3 had the best therapeutic potential showing both the lowest MIC values against the tested Candida strains and the non-toxic in vivo response in the zebrafish embryos at these doses.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Inorganic Biochemistry",
title = "New polynuclear 1,5-naphthyridine-silver(I) complexes as potential antimicrobial agents: The key role of the nature of donor coordinated to the metal center",
volume = "203",
doi = "10.1016/j.jinorgbio.2019.110872"
}

Durić, S., Vojnović, S., Pavić, A., Mojicević, M., Wadepohl, H., Savić, N. D., Popsavin, M., Nikodinović-Runić, J., Djuran, M.,& Glišić, B.. (2020). New polynuclear 1,5-naphthyridine-silver(I) complexes as potential antimicrobial agents: The key role of the nature of donor coordinated to the metal center. in Journal of Inorganic Biochemistry
Elsevier Science Inc, New York., 203.
https://doi.org/10.1016/j.jinorgbio.2019.110872

Durić S, Vojnović S, Pavić A, Mojicević M, Wadepohl H, Savić ND, Popsavin M, Nikodinović-Runić J, Djuran M, Glišić B. New polynuclear 1,5-naphthyridine-silver(I) complexes as potential antimicrobial agents: The key role of the nature of donor coordinated to the metal center. in Journal of Inorganic Biochemistry. 2020;203.
doi:10.1016/j.jinorgbio.2019.110872 .

Durić, Sonja, Vojnović, Sandra, Pavić, Aleksandar, Mojicević, Marija, Wadepohl, Hubert, Savić, Nada D., Popsavin, Mirjana, Nikodinović-Runić, Jasmina, Djuran, Milos , Glišić, Biljana, "New polynuclear 1,5-naphthyridine-silver(I) complexes as potential antimicrobial agents: The key role of the nature of donor coordinated to the metal center" in Journal of Inorganic Biochemistry, 203 (2020),
https://doi.org/10.1016/j.jinorgbio.2019.110872 . .

TY  - JOUR
AU  - Mojicević, Marija
AU  - D'Agostino, Paul M.
AU  - Pavić, Aleksandar
AU  - Vojnović, Sandra
AU  - Senthamaraikannan, Ramsankar
AU  - Vasiljević, Branka
AU  - Gulder, Tobias A. M.
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1384
AB  - Applying a bioactivity-guided isolation approach, staurosporine was separated and identified as the active principle in the culture extract of the new isolate Streptomyces sp. BV410 collected from the chamomile rhizosphere. The biotechnological production of staurosporine by strain BV410 was optimized to yield 56 mg/L after 14 days of incubation in soy flour-glucose-starch-mannitol-based fermentation medium (JS). The addition of FeSO4 significantly improved the staurosporine yield by 30%, while the addition of ZnSO4 significantly reduced staurosporine yield by 62% in comparison with the starting conditions. Although staurosporine was first isolated in 1977 from Lentzea albida (now Streptomyces staurosporeus) and its potent kinase inhibitory effect has been established, here, the biological activity of this natural product was assessed in depth in vivo using a selection of transgenic zebrafish (Danio rerio) models, including Tg(fli1:EGFP) with green fluorescent protein-labeled endothelial cells allowing visualization and monitoring of blood vessels. This confirmed a remarkable antiangiogenic activity of the compound at doses of 1 ng/ml (2.14 nmol/L) which is below doses inducing toxic effects (45 ng/ml; 75 nmol/L). A new, efficient producing strain of commercially significant staurosporine has been described along with optimized fermentation conditions, which may lead to optimization of the staurosporine scaffold and its wider applicability.
PB  - Wiley, Hoboken
T2  - Microbiologyopen
T1  - Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties
IS  - 3
VL  - 9
DO  - 10.1002/mbo3.986
ER  - 

@article{
author = "Mojicević, Marija and D'Agostino, Paul M. and Pavić, Aleksandar and Vojnović, Sandra and Senthamaraikannan, Ramsankar and Vasiljević, Branka and Gulder, Tobias A. M. and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "Applying a bioactivity-guided isolation approach, staurosporine was separated and identified as the active principle in the culture extract of the new isolate Streptomyces sp. BV410 collected from the chamomile rhizosphere. The biotechnological production of staurosporine by strain BV410 was optimized to yield 56 mg/L after 14 days of incubation in soy flour-glucose-starch-mannitol-based fermentation medium (JS). The addition of FeSO4 significantly improved the staurosporine yield by 30%, while the addition of ZnSO4 significantly reduced staurosporine yield by 62% in comparison with the starting conditions. Although staurosporine was first isolated in 1977 from Lentzea albida (now Streptomyces staurosporeus) and its potent kinase inhibitory effect has been established, here, the biological activity of this natural product was assessed in depth in vivo using a selection of transgenic zebrafish (Danio rerio) models, including Tg(fli1:EGFP) with green fluorescent protein-labeled endothelial cells allowing visualization and monitoring of blood vessels. This confirmed a remarkable antiangiogenic activity of the compound at doses of 1 ng/ml (2.14 nmol/L) which is below doses inducing toxic effects (45 ng/ml; 75 nmol/L). A new, efficient producing strain of commercially significant staurosporine has been described along with optimized fermentation conditions, which may lead to optimization of the staurosporine scaffold and its wider applicability.",
publisher = "Wiley, Hoboken",
journal = "Microbiologyopen",
title = "Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties",
number = "3",
volume = "9",
doi = "10.1002/mbo3.986"
}

Mojicević, M., D'Agostino, P. M., Pavić, A., Vojnović, S., Senthamaraikannan, R., Vasiljević, B., Gulder, T. A. M.,& Nikodinović-Runić, J.. (2020). Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties. in Microbiologyopen
Wiley, Hoboken., 9(3).
https://doi.org/10.1002/mbo3.986

Mojicević M, D'Agostino PM, Pavić A, Vojnović S, Senthamaraikannan R, Vasiljević B, Gulder TAM, Nikodinović-Runić J. Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties. in Microbiologyopen. 2020;9(3).
doi:10.1002/mbo3.986 .

Mojicević, Marija, D'Agostino, Paul M., Pavić, Aleksandar, Vojnović, Sandra, Senthamaraikannan, Ramsankar, Vasiljević, Branka, Gulder, Tobias A. M., Nikodinović-Runić, Jasmina, "Streptomyces sp. BV410 isolate from chamomile rhizosphere soil efficiently produces staurosporine with antifungal and antiangiogenic properties" in Microbiologyopen, 9, no. 3 (2020),
https://doi.org/10.1002/mbo3.986 . .

TY  - JOUR
AU  - Durić, Sonja Z.
AU  - Vojnović, Sandra
AU  - Andrejević, Tina P.
AU  - Stevanović, Nevena Lj.
AU  - Savić, Nada D.
AU  - Nikodinović-Runić, Jasmina
AU  - Glišić, Biljana
AU  - Djuran, Milos 
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1401
AB  - 1,2-Bis(4-pyridyl)ethane (bpa) and 1,2-bis(4-pyridyl)ethene (bpe) were used for the synthesis of polynuclear silver(I) complexes, {[Ag(bpa)]NO3}(n) (1), {[Ag(bpa)(2)](CF3SO3H2O)-H-.}(n) (2) and {[Ag(bpe)]CF3SO3}(n) (3). In complexes 1-3, the corresponding nitrogen-containing heterocycle acts as a bridging ligand between two Ag(I) ions. In vitro antimicrobial activity of these complexes, along with the ligands used for their synthesis, was evaluated against the broad panel of Gram-positive and Gram-negative bacteria and fungi. The silver(I) complexes 1-3 showed selectivity towards Candida spp. and Gram-negative Escherichia coli in comparison to the other investigated bacterial strains, effectively inhibiting the growth of four different Candida species with minimal inhibitory concentrations (MICs) between 2.5 and 25 mu g/mL and the growth of E. coli, with MIC value being 12.5 mu g/mL. Importantly, complex 2 significantly reduced C. albicans filamentation, an essential process for its pathogenesis. Antiproliferative effect on the normal human lung fibroblast cell line MRC-5 was also evaluated with the aim of determining the therapeutic potential of the complexes 1-3. The interactions of these complexes with calf thymus DNA (ctDNA) and bovine serum albumin (BSA) were studied to evaluate their binding activities towards these biomolecules for possible insights on their mode of action.
PB  - Hindawi Ltd, London
T2  - Bioinorganic Chemistry and Applications
T1  - Antimicrobial Activity and DNA/BSA Binding Affinity of Polynuclear Silver(I) Complexes with 1,2-Bis(4-pyridyl)ethane/ethene as Bridging Ligands
VL  - 2020
DO  - 10.1155/2020/3812050
ER  - 

@article{
author = "Durić, Sonja Z. and Vojnović, Sandra and Andrejević, Tina P. and Stevanović, Nevena Lj. and Savić, Nada D. and Nikodinović-Runić, Jasmina and Glišić, Biljana and Djuran, Milos ",
year = "2020",
abstract = "1,2-Bis(4-pyridyl)ethane (bpa) and 1,2-bis(4-pyridyl)ethene (bpe) were used for the synthesis of polynuclear silver(I) complexes, {[Ag(bpa)]NO3}(n) (1), {[Ag(bpa)(2)](CF3SO3H2O)-H-.}(n) (2) and {[Ag(bpe)]CF3SO3}(n) (3). In complexes 1-3, the corresponding nitrogen-containing heterocycle acts as a bridging ligand between two Ag(I) ions. In vitro antimicrobial activity of these complexes, along with the ligands used for their synthesis, was evaluated against the broad panel of Gram-positive and Gram-negative bacteria and fungi. The silver(I) complexes 1-3 showed selectivity towards Candida spp. and Gram-negative Escherichia coli in comparison to the other investigated bacterial strains, effectively inhibiting the growth of four different Candida species with minimal inhibitory concentrations (MICs) between 2.5 and 25 mu g/mL and the growth of E. coli, with MIC value being 12.5 mu g/mL. Importantly, complex 2 significantly reduced C. albicans filamentation, an essential process for its pathogenesis. Antiproliferative effect on the normal human lung fibroblast cell line MRC-5 was also evaluated with the aim of determining the therapeutic potential of the complexes 1-3. The interactions of these complexes with calf thymus DNA (ctDNA) and bovine serum albumin (BSA) were studied to evaluate their binding activities towards these biomolecules for possible insights on their mode of action.",
publisher = "Hindawi Ltd, London",
journal = "Bioinorganic Chemistry and Applications",
title = "Antimicrobial Activity and DNA/BSA Binding Affinity of Polynuclear Silver(I) Complexes with 1,2-Bis(4-pyridyl)ethane/ethene as Bridging Ligands",
volume = "2020",
doi = "10.1155/2020/3812050"
}

Durić, S. Z., Vojnović, S., Andrejević, T. P., Stevanović, N. Lj., Savić, N. D., Nikodinović-Runić, J., Glišić, B.,& Djuran, M.. (2020). Antimicrobial Activity and DNA/BSA Binding Affinity of Polynuclear Silver(I) Complexes with 1,2-Bis(4-pyridyl)ethane/ethene as Bridging Ligands. in Bioinorganic Chemistry and Applications
Hindawi Ltd, London., 2020.
https://doi.org/10.1155/2020/3812050

Durić SZ, Vojnović S, Andrejević TP, Stevanović NL, Savić ND, Nikodinović-Runić J, Glišić B, Djuran M. Antimicrobial Activity and DNA/BSA Binding Affinity of Polynuclear Silver(I) Complexes with 1,2-Bis(4-pyridyl)ethane/ethene as Bridging Ligands. in Bioinorganic Chemistry and Applications. 2020;2020.
doi:10.1155/2020/3812050 .

Durić, Sonja Z., Vojnović, Sandra, Andrejević, Tina P., Stevanović, Nevena Lj., Savić, Nada D., Nikodinović-Runić, Jasmina, Glišić, Biljana, Djuran, Milos , "Antimicrobial Activity and DNA/BSA Binding Affinity of Polynuclear Silver(I) Complexes with 1,2-Bis(4-pyridyl)ethane/ethene as Bridging Ligands" in Bioinorganic Chemistry and Applications, 2020 (2020),
https://doi.org/10.1155/2020/3812050 . .

TY  - JOUR
AU  - Delasoie, Joachim
AU  - Pavić, Aleksandar
AU  - Voutier, Noemie
AU  - Vojnović, Sandra
AU  - Crochet, Aurelien
AU  - Nikodinović-Runić, Jasmina
AU  - Zobi, Fabio
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1612
AB  - Combination therapy targeting both tumor growth and vascularization is considered to be a cornerstone for colorectal carcinomas (CRC) treatment. However, the major obstacles of most clinical anticancer drugs are their weak selective activity towards cancer cells and inherent inner organs toxicity, accompanied with fast drug resistance development. In our effort to discover novel selective and non-toxic agents effective against CRC, we designed, synthesized and characterized a series of rhenium(I) tricarbonyl-based complexes with increased lipophilicity. Two of these novel compounds were discovered to possess remarkable anticancer, anti-angiogenic and antimetastatic activity in vivo (zebrafish-human HCT-116 xenograft model), being effective at very low doses (1-3 mu M). At doses as high as 250 mu M the complexes did not provoke toxicity issues encountered in clinical anticancer drugs (cardio-, hepato-, and myelotoxicity). In vivo assays showed that the two compounds exceed the anti-tumor and anti-angiogenic activity of clinical drugs cisplatin and sunitinib malate, and display a large therapeutic window.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - European Journal of Medicinal Chemistry
T1  - Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma
VL  - 204
DO  - 10.1016/j.ejmech.2020.112583
ER  - 

@article{
author = "Delasoie, Joachim and Pavić, Aleksandar and Voutier, Noemie and Vojnović, Sandra and Crochet, Aurelien and Nikodinović-Runić, Jasmina and Zobi, Fabio",
year = "2020",
abstract = "Combination therapy targeting both tumor growth and vascularization is considered to be a cornerstone for colorectal carcinomas (CRC) treatment. However, the major obstacles of most clinical anticancer drugs are their weak selective activity towards cancer cells and inherent inner organs toxicity, accompanied with fast drug resistance development. In our effort to discover novel selective and non-toxic agents effective against CRC, we designed, synthesized and characterized a series of rhenium(I) tricarbonyl-based complexes with increased lipophilicity. Two of these novel compounds were discovered to possess remarkable anticancer, anti-angiogenic and antimetastatic activity in vivo (zebrafish-human HCT-116 xenograft model), being effective at very low doses (1-3 mu M). At doses as high as 250 mu M the complexes did not provoke toxicity issues encountered in clinical anticancer drugs (cardio-, hepato-, and myelotoxicity). In vivo assays showed that the two compounds exceed the anti-tumor and anti-angiogenic activity of clinical drugs cisplatin and sunitinib malate, and display a large therapeutic window.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "European Journal of Medicinal Chemistry",
title = "Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma",
volume = "204",
doi = "10.1016/j.ejmech.2020.112583"
}

Delasoie, J., Pavić, A., Voutier, N., Vojnović, S., Crochet, A., Nikodinović-Runić, J.,& Zobi, F.. (2020). Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 204.
https://doi.org/10.1016/j.ejmech.2020.112583

Delasoie J, Pavić A, Voutier N, Vojnović S, Crochet A, Nikodinović-Runić J, Zobi F. Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma. in European Journal of Medicinal Chemistry. 2020;204.
doi:10.1016/j.ejmech.2020.112583 .

Delasoie, Joachim, Pavić, Aleksandar, Voutier, Noemie, Vojnović, Sandra, Crochet, Aurelien, Nikodinović-Runić, Jasmina, Zobi, Fabio, "Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma" in European Journal of Medicinal Chemistry, 204 (2020),
https://doi.org/10.1016/j.ejmech.2020.112583 . .

TY  - JOUR
AU  - Delasoie, Joachim
AU  - Radaković, Nataša
AU  - Pavić, Aleksandar
AU  - Zobi, Fabio
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1335
AB  - Silica microparticles made of diatomaceous earth have become particularly attractive materials for designing drug delivery systems. In order to investigate the use of natural diatoms as drug scaffolds for carbon monoxide releasing molecules (CORMs), we evaluated the chemisorption of the cis-[Re(CO)(2)Br-4](2-) complex (ReCORM-2) and its vitamin B-12 derivative (B-12-ReCORM-2) on Coscinodiscus frustules by 3D FT-IR spectroscopic imaging, and the drugs' neovascularization effects in vivo in the zebrafish (Danio rerio) model. By mapping the symmetric Re-C equivalent to O upsilon(CO) stretching vibration of the CORMs in the 2000 cm(-1) region, we found that the drugs are mostly localized at the girdle band of the diatom frustule. Both ReCORM-2 and B-12-ReCORM-2 retain their CO-releasing ability when chemisorbed on the diatoms. When applied in vivo at doses  gt = 25 mu M, the molecules markedly reduced intersegmental and subintestinal vessels development in zebrafish, revealing high anti-angiogenic potential. In addition, diatom frustules did not provoke any toxic in vivo response in the zebrafish embryos, including inflammation. Overall, our results indicate that: (1) CORMs chemisorbed on diatom frustules retain their CO-releasing abilities; (2) both CO-releasing molecules show a concentration-dependent effect on the neovascularization in developing zebrafish; (3) silicate frustules are not toxic and could be used as CORMs drug carriers.
PB  - MDPI, Basel
T2  - Applied Sciences-Basel
T1  - Neovascularization Effects of Carbon Monoxide Releasing Drugs Chemisorbed on Coscinodiscus Diatoms Carriers Characterized by Spectromicroscopy Imaging
IS  - 20
VL  - 10
DO  - 10.3390/app10207380
ER  - 

@article{
author = "Delasoie, Joachim and Radaković, Nataša and Pavić, Aleksandar and Zobi, Fabio",
year = "2020",
abstract = "Silica microparticles made of diatomaceous earth have become particularly attractive materials for designing drug delivery systems. In order to investigate the use of natural diatoms as drug scaffolds for carbon monoxide releasing molecules (CORMs), we evaluated the chemisorption of the cis-[Re(CO)(2)Br-4](2-) complex (ReCORM-2) and its vitamin B-12 derivative (B-12-ReCORM-2) on Coscinodiscus frustules by 3D FT-IR spectroscopic imaging, and the drugs' neovascularization effects in vivo in the zebrafish (Danio rerio) model. By mapping the symmetric Re-C equivalent to O upsilon(CO) stretching vibration of the CORMs in the 2000 cm(-1) region, we found that the drugs are mostly localized at the girdle band of the diatom frustule. Both ReCORM-2 and B-12-ReCORM-2 retain their CO-releasing ability when chemisorbed on the diatoms. When applied in vivo at doses  gt = 25 mu M, the molecules markedly reduced intersegmental and subintestinal vessels development in zebrafish, revealing high anti-angiogenic potential. In addition, diatom frustules did not provoke any toxic in vivo response in the zebrafish embryos, including inflammation. Overall, our results indicate that: (1) CORMs chemisorbed on diatom frustules retain their CO-releasing abilities; (2) both CO-releasing molecules show a concentration-dependent effect on the neovascularization in developing zebrafish; (3) silicate frustules are not toxic and could be used as CORMs drug carriers.",
publisher = "MDPI, Basel",
journal = "Applied Sciences-Basel",
title = "Neovascularization Effects of Carbon Monoxide Releasing Drugs Chemisorbed on Coscinodiscus Diatoms Carriers Characterized by Spectromicroscopy Imaging",
number = "20",
volume = "10",
doi = "10.3390/app10207380"
}

Delasoie, J., Radaković, N., Pavić, A.,& Zobi, F.. (2020). Neovascularization Effects of Carbon Monoxide Releasing Drugs Chemisorbed on Coscinodiscus Diatoms Carriers Characterized by Spectromicroscopy Imaging. in Applied Sciences-Basel
MDPI, Basel., 10(20).
https://doi.org/10.3390/app10207380

Delasoie J, Radaković N, Pavić A, Zobi F. Neovascularization Effects of Carbon Monoxide Releasing Drugs Chemisorbed on Coscinodiscus Diatoms Carriers Characterized by Spectromicroscopy Imaging. in Applied Sciences-Basel. 2020;10(20).
doi:10.3390/app10207380 .

Delasoie, Joachim, Radaković, Nataša, Pavić, Aleksandar, Zobi, Fabio, "Neovascularization Effects of Carbon Monoxide Releasing Drugs Chemisorbed on Coscinodiscus Diatoms Carriers Characterized by Spectromicroscopy Imaging" in Applied Sciences-Basel, 10, no. 20 (2020),
https://doi.org/10.3390/app10207380 . .

TY  - JOUR
AU  - Simić, Stefan
AU  - Jeremić, Sanja
AU  - Đokić, Lidija
AU  - Bozić, Nataša
AU  - Vujcić, Zoran
AU  - Loncar, Nikola
AU  - Senthamaraikannan, Ramsankar
AU  - Babu, Ramesh
AU  - Opsenica, Igor M.
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1406
AB  - Biocatalytic oxidations mediated by laccases are gaining importance due to their versatility and beneficial environmental effects. In this study, the oxidation of 1,4-dihydropyridines has been performed using three different types of bacterial laccase-based catalysts: purified laccase from Bacillus licheniformis ATCC 9945a (BliLacc), Escherichia coli whole cells expressing this laccase, and bacterial nanocellulose (BNC) supported BliLacc catalysts. The catalysts based on bacterial laccase were compared to the commercially available Trametes versicolor laccase (TvLacc). The oxidation product of 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate was obtained within 7-24 h with good yields (70-99%) with all three biocatalysts. The substrate scope was examined with five additional 1,4-dihydropyridines, one of which was oxidized in high yield. Whole-cell biocatalyst was stable when stored for up to 1-month at 4 degrees C. In addition, evidence has been provided that multicopper oxidase CueO from the E. coli expression host contributed to the oxidation efficiency of the whole-cell biocatalyst. The immobilized whole-cell biocatalyst showed satisfactory activity and retained 37% of its original activity after three biotransformation cycles.
PB  - New York : Elsevier Science Inc
T2  - Enzyme and Microbial Technology
T1  - Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines
VL  - 132
DO  - 10.1016/j.enzmictec.2019.109411
ER  - 

@article{
author = "Simić, Stefan and Jeremić, Sanja and Đokić, Lidija and Bozić, Nataša and Vujcić, Zoran and Loncar, Nikola and Senthamaraikannan, Ramsankar and Babu, Ramesh and Opsenica, Igor M. and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "Biocatalytic oxidations mediated by laccases are gaining importance due to their versatility and beneficial environmental effects. In this study, the oxidation of 1,4-dihydropyridines has been performed using three different types of bacterial laccase-based catalysts: purified laccase from Bacillus licheniformis ATCC 9945a (BliLacc), Escherichia coli whole cells expressing this laccase, and bacterial nanocellulose (BNC) supported BliLacc catalysts. The catalysts based on bacterial laccase were compared to the commercially available Trametes versicolor laccase (TvLacc). The oxidation product of 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate was obtained within 7-24 h with good yields (70-99%) with all three biocatalysts. The substrate scope was examined with five additional 1,4-dihydropyridines, one of which was oxidized in high yield. Whole-cell biocatalyst was stable when stored for up to 1-month at 4 degrees C. In addition, evidence has been provided that multicopper oxidase CueO from the E. coli expression host contributed to the oxidation efficiency of the whole-cell biocatalyst. The immobilized whole-cell biocatalyst showed satisfactory activity and retained 37% of its original activity after three biotransformation cycles.",
publisher = "New York : Elsevier Science Inc",
journal = "Enzyme and Microbial Technology",
title = "Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines",
volume = "132",
doi = "10.1016/j.enzmictec.2019.109411"
}

Simić, S., Jeremić, S., Đokić, L., Bozić, N., Vujcić, Z., Loncar, N., Senthamaraikannan, R., Babu, R., Opsenica, I. M.,& Nikodinović-Runić, J.. (2020). Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines. in Enzyme and Microbial Technology
New York : Elsevier Science Inc., 132.
https://doi.org/10.1016/j.enzmictec.2019.109411

Simić S, Jeremić S, Đokić L, Bozić N, Vujcić Z, Loncar N, Senthamaraikannan R, Babu R, Opsenica IM, Nikodinović-Runić J. Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines. in Enzyme and Microbial Technology. 2020;132.
doi:10.1016/j.enzmictec.2019.109411 .

Simić, Stefan, Jeremić, Sanja, Đokić, Lidija, Bozić, Nataša, Vujcić, Zoran, Loncar, Nikola, Senthamaraikannan, Ramsankar, Babu, Ramesh, Opsenica, Igor M., Nikodinović-Runić, Jasmina, "Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines" in Enzyme and Microbial Technology, 132 (2020),
https://doi.org/10.1016/j.enzmictec.2019.109411 . .

TY  - JOUR
AU  - Sovari, Sara Nasiri
AU  - Vojnović, Sandra
AU  - Škaro Bogojević, Sanja
AU  - Crochet, Aurelien
AU  - Pavić, Aleksandar
AU  - Nikodinović-Runić, Jasmina
AU  - Zobi, Fabio
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1365
AB  - We have prepared a series of ten 3-arylcoumarin molecules, their respective fac-[Re(CO)(3)(bpy)L](+) and fac-[Re(CO)(3)(L(sic)L)Br] complexes and tested all compounds for their antimicrobial efficacy. Whereas the 3-arylcoumarin ligands are virtually inactive against the human-associated pathogens with minimum inhibitory concentrations (MICs)  gt  150 mu M, when coordinated to the fac-[Re(CO)(3)](+) core, most of the resulting complexes showed remarkable antibacterial potency. Several rhenium complexes exhibit activity in nanomolar concentrations against Gram-positive pathogens such as Staphylococcus aureus strains, including methicillin-resistant S. aureus (MRSA) and Enterococcus faecium. The molecules do not affect bacterial cell membrane potential, but some of the most potent complexes strongly interact with DNA, indicating it as a possible target for their mode of action. In vivo studies in the zebrafish model showed that the complexes with anti-staphylococcal/MRSA activity were non-toxic to the organism even at much higher doses of the corresponding MICs. In the zebrafish-MRSA infection model, the complexes increased the survival rate of infected fish up to 100% and markedly reduced bacterial burden. Moreover, all rescued fish developed normally following the treatments with the metallic compounds.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - European Journal of Medicinal Chemistry
T1  - Design, synthesis and in vivo evaluation of 3-arylcoumarin derivatives of rhenium(I) tricarbonyl complexes as potent antibacterial agents against methicillin-resistant Staphylococcus aureus (MRSA)
VL  - 205
DO  - 10.1016/j.ejmech.2020.112533
ER  - 

@article{
author = "Sovari, Sara Nasiri and Vojnović, Sandra and Škaro Bogojević, Sanja and Crochet, Aurelien and Pavić, Aleksandar and Nikodinović-Runić, Jasmina and Zobi, Fabio",
year = "2020",
abstract = "We have prepared a series of ten 3-arylcoumarin molecules, their respective fac-[Re(CO)(3)(bpy)L](+) and fac-[Re(CO)(3)(L(sic)L)Br] complexes and tested all compounds for their antimicrobial efficacy. Whereas the 3-arylcoumarin ligands are virtually inactive against the human-associated pathogens with minimum inhibitory concentrations (MICs)  gt  150 mu M, when coordinated to the fac-[Re(CO)(3)](+) core, most of the resulting complexes showed remarkable antibacterial potency. Several rhenium complexes exhibit activity in nanomolar concentrations against Gram-positive pathogens such as Staphylococcus aureus strains, including methicillin-resistant S. aureus (MRSA) and Enterococcus faecium. The molecules do not affect bacterial cell membrane potential, but some of the most potent complexes strongly interact with DNA, indicating it as a possible target for their mode of action. In vivo studies in the zebrafish model showed that the complexes with anti-staphylococcal/MRSA activity were non-toxic to the organism even at much higher doses of the corresponding MICs. In the zebrafish-MRSA infection model, the complexes increased the survival rate of infected fish up to 100% and markedly reduced bacterial burden. Moreover, all rescued fish developed normally following the treatments with the metallic compounds.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "European Journal of Medicinal Chemistry",
title = "Design, synthesis and in vivo evaluation of 3-arylcoumarin derivatives of rhenium(I) tricarbonyl complexes as potent antibacterial agents against methicillin-resistant Staphylococcus aureus (MRSA)",
volume = "205",
doi = "10.1016/j.ejmech.2020.112533"
}

Sovari, S. N., Vojnović, S., Škaro Bogojević, S., Crochet, A., Pavić, A., Nikodinović-Runić, J.,& Zobi, F.. (2020). Design, synthesis and in vivo evaluation of 3-arylcoumarin derivatives of rhenium(I) tricarbonyl complexes as potent antibacterial agents against methicillin-resistant Staphylococcus aureus (MRSA). in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 205.
https://doi.org/10.1016/j.ejmech.2020.112533

Sovari SN, Vojnović S, Škaro Bogojević S, Crochet A, Pavić A, Nikodinović-Runić J, Zobi F. Design, synthesis and in vivo evaluation of 3-arylcoumarin derivatives of rhenium(I) tricarbonyl complexes as potent antibacterial agents against methicillin-resistant Staphylococcus aureus (MRSA). in European Journal of Medicinal Chemistry. 2020;205.
doi:10.1016/j.ejmech.2020.112533 .

Sovari, Sara Nasiri, Vojnović, Sandra, Škaro Bogojević, Sanja, Crochet, Aurelien, Pavić, Aleksandar, Nikodinović-Runić, Jasmina, Zobi, Fabio, "Design, synthesis and in vivo evaluation of 3-arylcoumarin derivatives of rhenium(I) tricarbonyl complexes as potent antibacterial agents against methicillin-resistant Staphylococcus aureus (MRSA)" in European Journal of Medicinal Chemistry, 205 (2020),
https://doi.org/10.1016/j.ejmech.2020.112533 . .

TY  - JOUR
AU  - Delasoie, Joachim
AU  - Pavić, Aleksandar
AU  - Voutier, Noemie
AU  - Vojnović, Sandra
AU  - Crochet, Aurelien
AU  - Nikodinović-Runić, Jasmina
AU  - Zobi, Fabio
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1360
AB  - Combination therapy targeting both tumor growth and vascularization is considered to be a cornerstone for colorectal carcinomas (CRC) treatment. However, the major obstacles of most clinical anticancer drugs are their weak selective activity towards cancer cells and inherent inner organs toxicity, accompanied with fast drug resistance development. In our effort to discover novel selective and non-toxic agents effective against CRC, we designed, synthesized and characterized a series of rhenium(I) tricarbonyl-based complexes with increased lipophilicity. Two of these novel compounds were discovered to possess remarkable anticancer, anti-angiogenic and antimetastatic activity in vivo (zebrafish-human HCT-116 xenograft model), being effective at very low doses (1-3 mu M). At doses as high as 250 mu M the complexes did not provoke toxicity issues encountered in clinical anticancer drugs (cardio-, hepato-, and myelotoxicity). In vivo assays showed that the two compounds exceed the anti-tumor and anti-angiogenic activity of clinical drugs cisplatin and sunitinib malate, and display a large therapeutic window.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - European Journal of Medicinal Chemistry
T1  - Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma
VL  - 204
DO  - 10.1016/j.ejmech.2020.112583
ER  - 

@article{
author = "Delasoie, Joachim and Pavić, Aleksandar and Voutier, Noemie and Vojnović, Sandra and Crochet, Aurelien and Nikodinović-Runić, Jasmina and Zobi, Fabio",
year = "2020",
abstract = "Combination therapy targeting both tumor growth and vascularization is considered to be a cornerstone for colorectal carcinomas (CRC) treatment. However, the major obstacles of most clinical anticancer drugs are their weak selective activity towards cancer cells and inherent inner organs toxicity, accompanied with fast drug resistance development. In our effort to discover novel selective and non-toxic agents effective against CRC, we designed, synthesized and characterized a series of rhenium(I) tricarbonyl-based complexes with increased lipophilicity. Two of these novel compounds were discovered to possess remarkable anticancer, anti-angiogenic and antimetastatic activity in vivo (zebrafish-human HCT-116 xenograft model), being effective at very low doses (1-3 mu M). At doses as high as 250 mu M the complexes did not provoke toxicity issues encountered in clinical anticancer drugs (cardio-, hepato-, and myelotoxicity). In vivo assays showed that the two compounds exceed the anti-tumor and anti-angiogenic activity of clinical drugs cisplatin and sunitinib malate, and display a large therapeutic window.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "European Journal of Medicinal Chemistry",
title = "Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma",
volume = "204",
doi = "10.1016/j.ejmech.2020.112583"
}

Delasoie, J., Pavić, A., Voutier, N., Vojnović, S., Crochet, A., Nikodinović-Runić, J.,& Zobi, F.. (2020). Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 204.
https://doi.org/10.1016/j.ejmech.2020.112583

Delasoie J, Pavić A, Voutier N, Vojnović S, Crochet A, Nikodinović-Runić J, Zobi F. Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma. in European Journal of Medicinal Chemistry. 2020;204.
doi:10.1016/j.ejmech.2020.112583 .

Delasoie, Joachim, Pavić, Aleksandar, Voutier, Noemie, Vojnović, Sandra, Crochet, Aurelien, Nikodinović-Runić, Jasmina, Zobi, Fabio, "Identification of novel potent and non-toxic anticancer, anti-angiogenic and antimetastatic rhenium complexes against colorectal carcinoma" in European Journal of Medicinal Chemistry, 204 (2020),
https://doi.org/10.1016/j.ejmech.2020.112583 . .

TY  - JOUR
AU  - Jovanović, Marina
AU  - Morić, Ivana
AU  - Nikolić, Biljana
AU  - Pavić, Aleksandar
AU  - Svircev, Emilija
AU  - Šenerović, Lidija
AU  - Mitić-Culafić, Dragana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1320
AB  - Many traditional remedies represent potential candidates for integration with modern medical practice, but credible data on their activities are often scarce. For the first time, the anti-virulence potential and the safety for human use of the ethanol extracts of two medicinal plants, Persicaria maculosa (PEM) and Bistorta officinalis (BIO), have been addressed. Ethanol extracts of both plants exhibited anti-virulence activity against the medically important opportunistic pathogen Pseudomonas aeruginosa. At the subinhibitory concentration of 50 mu g/mL, the extracts demonstrated a maximal inhibitory effect (approx. 50%) against biofilm formation, the highest reduction of pyocyanin production (47% for PEM and 59% for BIO) and completely halted the swarming motility of P. aeruginosa. Both extracts demonstrated better anti-quorum sensing and antibiofilm activities, and a better ability to interfere with LasR receptor, than the tested dominant extracts' constituents. The bioactive concentrations of the extracts were not toxic in the zebrafish model system. This study represents an initial step towards the integration of P. maculosa and B. officinalis for use in the treatment of Pseudomonas infections.
PB  - MDPI, Basel
T2  - Molecules
T1  - Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts
IS  - 8
VL  - 25
DO  - 10.3390/molecules25081811
ER  - 

@article{
author = "Jovanović, Marina and Morić, Ivana and Nikolić, Biljana and Pavić, Aleksandar and Svircev, Emilija and Šenerović, Lidija and Mitić-Culafić, Dragana",
year = "2020",
abstract = "Many traditional remedies represent potential candidates for integration with modern medical practice, but credible data on their activities are often scarce. For the first time, the anti-virulence potential and the safety for human use of the ethanol extracts of two medicinal plants, Persicaria maculosa (PEM) and Bistorta officinalis (BIO), have been addressed. Ethanol extracts of both plants exhibited anti-virulence activity against the medically important opportunistic pathogen Pseudomonas aeruginosa. At the subinhibitory concentration of 50 mu g/mL, the extracts demonstrated a maximal inhibitory effect (approx. 50%) against biofilm formation, the highest reduction of pyocyanin production (47% for PEM and 59% for BIO) and completely halted the swarming motility of P. aeruginosa. Both extracts demonstrated better anti-quorum sensing and antibiofilm activities, and a better ability to interfere with LasR receptor, than the tested dominant extracts' constituents. The bioactive concentrations of the extracts were not toxic in the zebrafish model system. This study represents an initial step towards the integration of P. maculosa and B. officinalis for use in the treatment of Pseudomonas infections.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts",
number = "8",
volume = "25",
doi = "10.3390/molecules25081811"
}

Jovanović, M., Morić, I., Nikolić, B., Pavić, A., Svircev, E., Šenerović, L.,& Mitić-Culafić, D.. (2020). Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts. in Molecules
MDPI, Basel., 25(8).
https://doi.org/10.3390/molecules25081811

Jovanović M, Morić I, Nikolić B, Pavić A, Svircev E, Šenerović L, Mitić-Culafić D. Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts. in Molecules. 2020;25(8).
doi:10.3390/molecules25081811 .

Jovanović, Marina, Morić, Ivana, Nikolić, Biljana, Pavić, Aleksandar, Svircev, Emilija, Šenerović, Lidija, Mitić-Culafić, Dragana, "Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts" in Molecules, 25, no. 8 (2020),
https://doi.org/10.3390/molecules25081811 . .

TY  - JOUR
AU  - Delasoie, Joachim
AU  - Schiel, Philippe
AU  - Vojnović, Sandra
AU  - Nikodinović-Runić, Jasmina
AU  - Zobi, Fabio
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1301
AB  - Systemic toxicity and severe side effects are commonly associated with anticancer chemotherapies. New strategies based on enhanced drug selectivity and targeted delivery to cancer cells while leaving healthy tissue undamaged can reduce the global patient burden. Herein, we report the design, synthesis and characterization of a bio-inspired hybrid multifunctional drug delivery system based on diatom microalgae. The microalgae's surface was chemically functionalized with hybrid vitamin B-12-photoactivatable molecules and the materials further loaded with highly active rhenium(I) tricarbonyl anticancer complexes. The constructs showed enhanced adherence to colorectal cancer (CRC) cells and slow release of the chemotherapeutic drugs. The overall toxicity of the hybrid multifunctional drug delivery system was further enhanced by photoactivation of the microalgae surface. Depending on the construct and anticancer drug, a 2-fold increase in the cytotoxic efficacy of the drug was observed upon light irradiation. The use of this targeted drug delivery strategy, together with selective spatial-temporal light activation, may lead to lower effective concentration of anticancer drugs, thereby reducing medication doses, possible side effects and overall burden for the patient.
PB  - MDPI, Basel
T2  - Pharmaceutics
T1  - Photoactivatable Surface-Functionalized Diatom Microalgae for Colorectal Cancer Targeted Delivery and Enhanced Cytotoxicity of Anticancer Complexes
IS  - 5
VL  - 12
DO  - 10.3390/pharmaceutics12050480
ER  - 

@article{
author = "Delasoie, Joachim and Schiel, Philippe and Vojnović, Sandra and Nikodinović-Runić, Jasmina and Zobi, Fabio",
year = "2020",
abstract = "Systemic toxicity and severe side effects are commonly associated with anticancer chemotherapies. New strategies based on enhanced drug selectivity and targeted delivery to cancer cells while leaving healthy tissue undamaged can reduce the global patient burden. Herein, we report the design, synthesis and characterization of a bio-inspired hybrid multifunctional drug delivery system based on diatom microalgae. The microalgae's surface was chemically functionalized with hybrid vitamin B-12-photoactivatable molecules and the materials further loaded with highly active rhenium(I) tricarbonyl anticancer complexes. The constructs showed enhanced adherence to colorectal cancer (CRC) cells and slow release of the chemotherapeutic drugs. The overall toxicity of the hybrid multifunctional drug delivery system was further enhanced by photoactivation of the microalgae surface. Depending on the construct and anticancer drug, a 2-fold increase in the cytotoxic efficacy of the drug was observed upon light irradiation. The use of this targeted drug delivery strategy, together with selective spatial-temporal light activation, may lead to lower effective concentration of anticancer drugs, thereby reducing medication doses, possible side effects and overall burden for the patient.",
publisher = "MDPI, Basel",
journal = "Pharmaceutics",
title = "Photoactivatable Surface-Functionalized Diatom Microalgae for Colorectal Cancer Targeted Delivery and Enhanced Cytotoxicity of Anticancer Complexes",
number = "5",
volume = "12",
doi = "10.3390/pharmaceutics12050480"
}

Delasoie, J., Schiel, P., Vojnović, S., Nikodinović-Runić, J.,& Zobi, F.. (2020). Photoactivatable Surface-Functionalized Diatom Microalgae for Colorectal Cancer Targeted Delivery and Enhanced Cytotoxicity of Anticancer Complexes. in Pharmaceutics
MDPI, Basel., 12(5).
https://doi.org/10.3390/pharmaceutics12050480

Delasoie J, Schiel P, Vojnović S, Nikodinović-Runić J, Zobi F. Photoactivatable Surface-Functionalized Diatom Microalgae for Colorectal Cancer Targeted Delivery and Enhanced Cytotoxicity of Anticancer Complexes. in Pharmaceutics. 2020;12(5).
doi:10.3390/pharmaceutics12050480 .

Delasoie, Joachim, Schiel, Philippe, Vojnović, Sandra, Nikodinović-Runić, Jasmina, Zobi, Fabio, "Photoactivatable Surface-Functionalized Diatom Microalgae for Colorectal Cancer Targeted Delivery and Enhanced Cytotoxicity of Anticancer Complexes" in Pharmaceutics, 12, no. 5 (2020),
https://doi.org/10.3390/pharmaceutics12050480 . .

TY  - JOUR
AU  - Durić, Sonja Z.
AU  - Mojicević, Marija
AU  - Vojnović, Sandra
AU  - Wadepohl, Hubert
AU  - Andrejević, Tina P.
AU  - Stevanović, Nevena Lj.
AU  - Nikodinović-Runić, Jasmina
AU  - Djuran, Milos
AU  - Glišić, Biljana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1369
AB  - In a continuing search for a novel metal-containing antimicrobial agents, the present study reports the synthesis, characterization and biological evaluation of two silver(I) complexes with 1,10-phenanthroline-based ligands, [Ag(1,10-phen)(2)]CF3COO center dot H2O (Ag1) and [Ag(CF3COO)(5,6-epoxy-1,10-phen)](2)(Ag2), 1,10-phen is 1, 10-phenanthroline and 5,6-epoxy-1,10-phen is 5,6-epoxy-5,6-dihydro-1,10-phenanthroline. The complexes were characterized by different spectroscopic techniques (IR, H-1 and C-13 NMR and UV-Vis), while the crystal structure of Ag2 complex was determined by a single-crystal X-ray diffraction analysis. The spectroscopic data confirmed that the structure of Ag1 complex, with silver(I) ion tetrahedrally coordinated by two bidentate 1, 10-phen ligands, is in accordance to the previous report (S.E. Paramonov et al., 2003). The crystallographic results showed that in dinuclear Ag2 complex, both Ag(I) ions are coordinated bidentately by 5,6-epoxy-1, 10-phen and monodentately by trifluoroacetate, with presence of the short Ag center dot center dot center dot Ag contact of 2.963 angstrom. Both silver (I) complexes were evaluated in vitro for antimicrobial activity against four bacterial and four Candida species, showing selectivity towards the investigated species of Candida with minimal inhibitory concentrations (MICs) between 0.9 and 12.5 mu M. Moreover, Ag2 complex manifested significant antiproliferative properties in the case of a range of human cell lines, including human breast cancer (MDA-MB 231), which resulted from the presence of epoxy functional group in the ligand. The gel electrophoresis results obtained from the studies of Ag1 and Ag2 interactions with bacteriophage lambda DNA (XDNA) suggested that these complexes did not cause DNA degradation.
PB  - Elsevier Science Sa, Lausanne
T2  - Inorganica Chimica Acta
T1  - Silver(I) complexes with 1,10-phenanthroline-based ligands: The influence of epoxide function on the complex structure and biological activity
VL  - 502
DO  - 10.1016/j.ica.2019.119357
ER  - 

@article{
author = "Durić, Sonja Z. and Mojicević, Marija and Vojnović, Sandra and Wadepohl, Hubert and Andrejević, Tina P. and Stevanović, Nevena Lj. and Nikodinović-Runić, Jasmina and Djuran, Milos and Glišić, Biljana",
year = "2020",
abstract = "In a continuing search for a novel metal-containing antimicrobial agents, the present study reports the synthesis, characterization and biological evaluation of two silver(I) complexes with 1,10-phenanthroline-based ligands, [Ag(1,10-phen)(2)]CF3COO center dot H2O (Ag1) and [Ag(CF3COO)(5,6-epoxy-1,10-phen)](2)(Ag2), 1,10-phen is 1, 10-phenanthroline and 5,6-epoxy-1,10-phen is 5,6-epoxy-5,6-dihydro-1,10-phenanthroline. The complexes were characterized by different spectroscopic techniques (IR, H-1 and C-13 NMR and UV-Vis), while the crystal structure of Ag2 complex was determined by a single-crystal X-ray diffraction analysis. The spectroscopic data confirmed that the structure of Ag1 complex, with silver(I) ion tetrahedrally coordinated by two bidentate 1, 10-phen ligands, is in accordance to the previous report (S.E. Paramonov et al., 2003). The crystallographic results showed that in dinuclear Ag2 complex, both Ag(I) ions are coordinated bidentately by 5,6-epoxy-1, 10-phen and monodentately by trifluoroacetate, with presence of the short Ag center dot center dot center dot Ag contact of 2.963 angstrom. Both silver (I) complexes were evaluated in vitro for antimicrobial activity against four bacterial and four Candida species, showing selectivity towards the investigated species of Candida with minimal inhibitory concentrations (MICs) between 0.9 and 12.5 mu M. Moreover, Ag2 complex manifested significant antiproliferative properties in the case of a range of human cell lines, including human breast cancer (MDA-MB 231), which resulted from the presence of epoxy functional group in the ligand. The gel electrophoresis results obtained from the studies of Ag1 and Ag2 interactions with bacteriophage lambda DNA (XDNA) suggested that these complexes did not cause DNA degradation.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Inorganica Chimica Acta",
title = "Silver(I) complexes with 1,10-phenanthroline-based ligands: The influence of epoxide function on the complex structure and biological activity",
volume = "502",
doi = "10.1016/j.ica.2019.119357"
}

Durić, S. Z., Mojicević, M., Vojnović, S., Wadepohl, H., Andrejević, T. P., Stevanović, N. Lj., Nikodinović-Runić, J., Djuran, M.,& Glišić, B.. (2020). Silver(I) complexes with 1,10-phenanthroline-based ligands: The influence of epoxide function on the complex structure and biological activity. in Inorganica Chimica Acta
Elsevier Science Sa, Lausanne., 502.
https://doi.org/10.1016/j.ica.2019.119357

Durić SZ, Mojicević M, Vojnović S, Wadepohl H, Andrejević TP, Stevanović NL, Nikodinović-Runić J, Djuran M, Glišić B. Silver(I) complexes with 1,10-phenanthroline-based ligands: The influence of epoxide function on the complex structure and biological activity. in Inorganica Chimica Acta. 2020;502.
doi:10.1016/j.ica.2019.119357 .

Durić, Sonja Z., Mojicević, Marija, Vojnović, Sandra, Wadepohl, Hubert, Andrejević, Tina P., Stevanović, Nevena Lj., Nikodinović-Runić, Jasmina, Djuran, Milos, Glišić, Biljana, "Silver(I) complexes with 1,10-phenanthroline-based ligands: The influence of epoxide function on the complex structure and biological activity" in Inorganica Chimica Acta, 502 (2020),
https://doi.org/10.1016/j.ica.2019.119357 . .

TY  - THES
AU  - Mojićević, Marija
PY  - 2019-12-13
UR  - https://nardus.mpn.gov.rs/handle/123456789/11581
UR  - https://www.cris.uns.ac.rs/DownloadFileServlet/Disertacija156992646214263.pdf?controlNumber=(BISIS)111226&fileName=156992646214263.pdf&id=13583&source=NaRDuS&language=sr
UR  - https://www.cris.uns.ac.rs/record.jsf?recordId=111226&source=NaRDuS&language=sr
UR  - https://www.cris.uns.ac.rs/DownloadFileServlet/IzvestajKomisije156992345335493.pdf?controlNumber=(BISIS)111226&fileName=156992345335493.pdf&id=13580&source=NaRDuS&language=sr
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2426
AB  - Zemljište predstavlja bogat izvor različitih mikroorganizama čiji produkti metabolizma mogu biti od izuzetnog značaja za čoveka. Dosadašnja ispitivanja mikrobnog diverziteta u zemljištu su otkrila bogati biosintetski potencijal za proizvodnju novih prirodnih proizvoda kod velikog broja mikroorganizama, naročito kada je u pitanju klasa Actinobacteria. Među zemljišnim izolatima, rod Streptomyces prednjači po broju identifikovanih bioaktivnih molekula u odnosu na sve ostale bakterije. Stoga je jedan od ciljeva u okviru ove doktorske disertacije izolacija streptomiceta iz rizosfera medicinski značajnih biljaka sakupljenih na teritoriji Republike Srbije (Papaver rhoeas, Matricaria chamomilla, i Urtica dioica) i ispitivanje njihovog antifungalnog potencijala na različite vrste kandida. Morfološki različiti izolati (ukupno 103) su izolovani iz uzoraka rizosfera i okarakterisani kao streptomicete. Dve različite podloge i dve procedure za ekstrakciju su korišćene da bi se pospešila detekcija antifungalnih jedinjenja. Ispitan je uticaj ukupno 412 ekstrakata na rast Candida albicans disk difuzionim esejem pri čemu je utvrđeno da 42% (43/103) izolata imaju sposobnost proizvodnje antifungalnih jedinjenja pri ispitivanim uslovima. Pojedini ekstrakti su inhibirali rast važnih humanih patogena poput Candida krusei, Candida parapsilosis, i Candida glabrata. Na osnovu stepena i spektra antifungalne aktivnosti devet izolata je odabrano za dalja istraživanja. Ispitana je sposobnost njihovih ekstrakata da inhibiraju rast kandida u tečnoj kulturi i u formi biofilma, a takođe je ispitan i njihov uticaj na već formirane biofilmove kandide u koncentracijama od 8 do 250 pg/ml. Hromatografski profili ovih ekstrakata i uvid u njihovu metaboličku raznolikost dobijeni su korišćenjem tečne hromatografije visokih performansi. Tri ekstrakta sa specifičnom antifungalnom aktivnošću podvrgnuta su hemijskim analizama s ciljem da se detektuju i strukturno okarakterišu molekuli koji su nosioci antifungalne aktivnosti. Na osnovu rezultata nuklearnomagnetno- rezonantne spektroskopije otkriveno je da su aktivni molekuli genistein, daidzein i staurosporin. Genistein i daidzein koji su poznati fitoestrogeni poreklom iz sojinog brašna za koje je poznato da inhibiraju ključne enzime u biosintetskom putu steroida. Njihovo prisustvo je u ovom istraživanju detektovano usled korišćenja sojinog brašna u hranljivoj podlozi. Kako streptomicete u čijim ekstraktima su detektovani ovi molekuli pokazuju sposobnost oslobađanja ovih važnih jedinjenja iz kompleksne hranljive podloge, mogu se uzeti u razmatranje za biotehnološku proizvodnju fitoestrogena. Staurosporin je detektovan kao nosilac antifungalne aktivnosti kod ekstrakta soja Streptomyces sp. BV410. Staurosporin je inhibitor protein kinaza i njegovi derivati i analozi se koriste u kao antitumorski agensi. Biosinteza ovog molekula je optimizovana do prinosa od 36,94 mg/l nakon 14 dana gajenja u hranljivoj podlozi koja sadrži glukozu, skrob, manitol i sojino brašno (JS). Dalja optimizacija hranljive podloge za biosintezu staurosporina ukazala je na sledeći sastav hranljive podloge: 20 g/l glukoze, 0,36 g/l skroba, 21,46 g/l manitola, 17,32 g/l sojinog brašna. Primenom definisanih optimalnih vrednosti i korišćenjem odgovarajućih matematičkih modela, predviđeno je da će se na ovaj način postići prinosi od 46,88 mg/l staurosporina i 12,05 mg/ml biomase. Validnost predviđenih rezultata potvrđena je izvođenjem bioprocesa u optimizovanoj hranljivoj podlozi (JSSta). Ispitana je kinetika biosinteze staurosporina i produkcije biomase, kao i potrošnje izvora ugljenika i razvijeni su odgovarajući procesni modeli. Dodatna optimizacija je podrazumevala dodatak suplemenata koji prema literature stimulišu sekundarni metabolizam streptomiceta (joni cinka, gvožđa, fosfati, metil oleat, ulje semenki grožđa). Ovi eksperimenti su izvođeni na tri pH vrednosti (6,5, 7,5 i 8,5) a uspešnost bioprocesa je procenjivana 7., 10. i 14. dana gajenja. Dodatna optimizacija je dovela do podatka da dodatak soli gvožđa značajno pospešuje biosintezu staurosporina sa povećanjem prinosa od 25%. Dobijeni rezultati potvrđuju da su rizosfere medicinski značajnih biljaka značajan izvor streptomiceta koje proizvode komponente sa antifungalnom aktivnošću. Izolacija novog proizvođača staurosporina i optimizacija procesa njegove biosinteze omogućiće dalja istraživanja ovog jedinjenja koje može biti osnova za razvoj novih antifungalnih i jedinjenja koja inhibiraju angiogenezu. Rezultati dobijeni u okviru ovih istraživanja predstavljaju početni korak ka potencijalnoj industrijalizaciji proizvodnje staurosporina.
AB  - Different soils are still a source of remarkable microbial diversity which also reflects in the unexplored chemical diversity. Recent advances in assessment of microbial diversity from soil have revealed the extraordinarily rich biosynthetic potential for the production of new natural products among different microbial strains, especially within the group of Actinobacteria. Among bacterial soil isolates, representatives of Streptomyces genus are the most prolific producers of bioactive compounds. One of the objectives of the present study was to isolate Streptomyces spp. from the rhizosphere soils of three ethno-medicinal plants collected in Serbia (Papaver rhoeas, Matricaria chamomilla, and Urtica dioica) and to screen their antifungal activity against Candida spp. Morphologically different sporulating isolates (103 in total) were collected from rhizosphere soil samples and determined as Streptomyces spp. Two different media and two extraction procedures were used to induce the production and facilitate identification of antifungals. Overall, 412 crude cell extracts were tested against Candida albicans using disk diffusion assays, with 42% (43/103) of the strains showing the ability to produce antifungal agents. Also, extracts inhibited growth of other important human pathogens: Candida krusei, Candida parapsilosis, and Candida glabrata. Based on the established degree and range of antifungal activity, nine isolates were selected for further testing. Their ability to inhibit Candida growth in liquid culture, to inhibit biofilm formation, and to disperse pre-formed biofilms was assessed with active concentrations from 8 to 250 pg/ml. High-performance liquid chromatographic profiles of extracts derived from selected strains were recorded, revealing moderate metabolic diversity. The most potent extracts were subjected to comprehensive identification and structural characterization of antifungal compounds. Applying a bioactivity-guided isolation approach, active compounds of three extracts were separated, and based on NMR structure elucidation it was shown that active compounds were genistein, daidzein and staurosporine. Genistein and daidzein, soy phytoestrogens, are known to inhibit key enzymes in the steroid metabolism pathway and were coming from the fermentation medium containing soy flower. Since isolated Streptomyces spp. showed good ability to extract these molecules from complex medium, they can be further considered for biotechnological production of these phytoestrogens. One of the isolates, Streptomyces sp. BV410, was characterized as an efficient staurosporine producer. Staurosporine is a potent inhibitor of protein kinases and is considered in anticancer therapy. The biotechnological production of staurosporine by strain BV410 was optimized to yield 36.94 mg/l after 14 days of incubation in soy flowerglucose- starch-mannitol based fermentation medium (JS). Further optimization of medium for biosynthesis of staurosporine indicated the following optimal values of the examined factors: the content of glucose of 20 g/l, starch 0.36 g/l, mannitol 21.46 g/l, soy flower 17.32 g/l. By applying the defined optimal values and using the appropriate mathematical models, the following responses were predicted: concentration of staurosporine 46.88 mg/l and biomass yield 12.05 mg/ml. The validity of the results was confirmed by performing the biosynthesis of the staurosporine in the medium with optimal composition (JSSta). Kinetics of staurosporine and biomass production and carbon source consumption were examined and process models were developed. Additionally, optimization of staurosporine production was performed with different supplements which, according to literature data, had stimulative effect on secondary metabolism (Zn, Fe and P salts, methyl oleate, grape seed oil). In order to improve the production of staurosporine, effects of pH (6.5, 7.5 and 8.5) and incubation time (7, 10 and 14 days) were also examined. It was found that addition of FeS04 significantly improved the staurosporine yield in comparison to the starting conditions (increase of 25%). Our results proved that rhizosphere soils of ethno-medicinal plants are a prolific source of streptomycetes, producers of compounds with good antifungal activity. Isolation of the new staurosporine producing strain, allowed for its detailed bioactivity assessment. Staurosporine scaffold might serve as a lead structure for the development of new antifungal and antiangiogenic agents. Also, results obtained within this research represent the basis for the further scale-up and potential industrialization of the proposed production process.
PB  - Универзитет у Новом Саду, Технолошки факултет
T2  - Универзитет у Новом Саду
T1  - Antifungalni potencijal streptomiceta izolovanih iz rizosfera medicinski značajnih biljaka: karakterizacija i optimizacija biosinteze staurosporina, produkta metabolizma Streptomyces sp. BV410
UR  - https://hdl.handle.net/21.15107/rcub_nardus_11581
ER  - 

@phdthesis{
author = "Mojićević, Marija",
year = "2019-12-13",
abstract = "Zemljište predstavlja bogat izvor različitih mikroorganizama čiji produkti metabolizma mogu biti od izuzetnog značaja za čoveka. Dosadašnja ispitivanja mikrobnog diverziteta u zemljištu su otkrila bogati biosintetski potencijal za proizvodnju novih prirodnih proizvoda kod velikog broja mikroorganizama, naročito kada je u pitanju klasa Actinobacteria. Među zemljišnim izolatima, rod Streptomyces prednjači po broju identifikovanih bioaktivnih molekula u odnosu na sve ostale bakterije. Stoga je jedan od ciljeva u okviru ove doktorske disertacije izolacija streptomiceta iz rizosfera medicinski značajnih biljaka sakupljenih na teritoriji Republike Srbije (Papaver rhoeas, Matricaria chamomilla, i Urtica dioica) i ispitivanje njihovog antifungalnog potencijala na različite vrste kandida. Morfološki različiti izolati (ukupno 103) su izolovani iz uzoraka rizosfera i okarakterisani kao streptomicete. Dve različite podloge i dve procedure za ekstrakciju su korišćene da bi se pospešila detekcija antifungalnih jedinjenja. Ispitan je uticaj ukupno 412 ekstrakata na rast Candida albicans disk difuzionim esejem pri čemu je utvrđeno da 42% (43/103) izolata imaju sposobnost proizvodnje antifungalnih jedinjenja pri ispitivanim uslovima. Pojedini ekstrakti su inhibirali rast važnih humanih patogena poput Candida krusei, Candida parapsilosis, i Candida glabrata. Na osnovu stepena i spektra antifungalne aktivnosti devet izolata je odabrano za dalja istraživanja. Ispitana je sposobnost njihovih ekstrakata da inhibiraju rast kandida u tečnoj kulturi i u formi biofilma, a takođe je ispitan i njihov uticaj na već formirane biofilmove kandide u koncentracijama od 8 do 250 pg/ml. Hromatografski profili ovih ekstrakata i uvid u njihovu metaboličku raznolikost dobijeni su korišćenjem tečne hromatografije visokih performansi. Tri ekstrakta sa specifičnom antifungalnom aktivnošću podvrgnuta su hemijskim analizama s ciljem da se detektuju i strukturno okarakterišu molekuli koji su nosioci antifungalne aktivnosti. Na osnovu rezultata nuklearnomagnetno- rezonantne spektroskopije otkriveno je da su aktivni molekuli genistein, daidzein i staurosporin. Genistein i daidzein koji su poznati fitoestrogeni poreklom iz sojinog brašna za koje je poznato da inhibiraju ključne enzime u biosintetskom putu steroida. Njihovo prisustvo je u ovom istraživanju detektovano usled korišćenja sojinog brašna u hranljivoj podlozi. Kako streptomicete u čijim ekstraktima su detektovani ovi molekuli pokazuju sposobnost oslobađanja ovih važnih jedinjenja iz kompleksne hranljive podloge, mogu se uzeti u razmatranje za biotehnološku proizvodnju fitoestrogena. Staurosporin je detektovan kao nosilac antifungalne aktivnosti kod ekstrakta soja Streptomyces sp. BV410. Staurosporin je inhibitor protein kinaza i njegovi derivati i analozi se koriste u kao antitumorski agensi. Biosinteza ovog molekula je optimizovana do prinosa od 36,94 mg/l nakon 14 dana gajenja u hranljivoj podlozi koja sadrži glukozu, skrob, manitol i sojino brašno (JS). Dalja optimizacija hranljive podloge za biosintezu staurosporina ukazala je na sledeći sastav hranljive podloge: 20 g/l glukoze, 0,36 g/l skroba, 21,46 g/l manitola, 17,32 g/l sojinog brašna. Primenom definisanih optimalnih vrednosti i korišćenjem odgovarajućih matematičkih modela, predviđeno je da će se na ovaj način postići prinosi od 46,88 mg/l staurosporina i 12,05 mg/ml biomase. Validnost predviđenih rezultata potvrđena je izvođenjem bioprocesa u optimizovanoj hranljivoj podlozi (JSSta). Ispitana je kinetika biosinteze staurosporina i produkcije biomase, kao i potrošnje izvora ugljenika i razvijeni su odgovarajući procesni modeli. Dodatna optimizacija je podrazumevala dodatak suplemenata koji prema literature stimulišu sekundarni metabolizam streptomiceta (joni cinka, gvožđa, fosfati, metil oleat, ulje semenki grožđa). Ovi eksperimenti su izvođeni na tri pH vrednosti (6,5, 7,5 i 8,5) a uspešnost bioprocesa je procenjivana 7., 10. i 14. dana gajenja. Dodatna optimizacija je dovela do podatka da dodatak soli gvožđa značajno pospešuje biosintezu staurosporina sa povećanjem prinosa od 25%. Dobijeni rezultati potvrđuju da su rizosfere medicinski značajnih biljaka značajan izvor streptomiceta koje proizvode komponente sa antifungalnom aktivnošću. Izolacija novog proizvođača staurosporina i optimizacija procesa njegove biosinteze omogućiće dalja istraživanja ovog jedinjenja koje može biti osnova za razvoj novih antifungalnih i jedinjenja koja inhibiraju angiogenezu. Rezultati dobijeni u okviru ovih istraživanja predstavljaju početni korak ka potencijalnoj industrijalizaciji proizvodnje staurosporina., Different soils are still a source of remarkable microbial diversity which also reflects in the unexplored chemical diversity. Recent advances in assessment of microbial diversity from soil have revealed the extraordinarily rich biosynthetic potential for the production of new natural products among different microbial strains, especially within the group of Actinobacteria. Among bacterial soil isolates, representatives of Streptomyces genus are the most prolific producers of bioactive compounds. One of the objectives of the present study was to isolate Streptomyces spp. from the rhizosphere soils of three ethno-medicinal plants collected in Serbia (Papaver rhoeas, Matricaria chamomilla, and Urtica dioica) and to screen their antifungal activity against Candida spp. Morphologically different sporulating isolates (103 in total) were collected from rhizosphere soil samples and determined as Streptomyces spp. Two different media and two extraction procedures were used to induce the production and facilitate identification of antifungals. Overall, 412 crude cell extracts were tested against Candida albicans using disk diffusion assays, with 42% (43/103) of the strains showing the ability to produce antifungal agents. Also, extracts inhibited growth of other important human pathogens: Candida krusei, Candida parapsilosis, and Candida glabrata. Based on the established degree and range of antifungal activity, nine isolates were selected for further testing. Their ability to inhibit Candida growth in liquid culture, to inhibit biofilm formation, and to disperse pre-formed biofilms was assessed with active concentrations from 8 to 250 pg/ml. High-performance liquid chromatographic profiles of extracts derived from selected strains were recorded, revealing moderate metabolic diversity. The most potent extracts were subjected to comprehensive identification and structural characterization of antifungal compounds. Applying a bioactivity-guided isolation approach, active compounds of three extracts were separated, and based on NMR structure elucidation it was shown that active compounds were genistein, daidzein and staurosporine. Genistein and daidzein, soy phytoestrogens, are known to inhibit key enzymes in the steroid metabolism pathway and were coming from the fermentation medium containing soy flower. Since isolated Streptomyces spp. showed good ability to extract these molecules from complex medium, they can be further considered for biotechnological production of these phytoestrogens. One of the isolates, Streptomyces sp. BV410, was characterized as an efficient staurosporine producer. Staurosporine is a potent inhibitor of protein kinases and is considered in anticancer therapy. The biotechnological production of staurosporine by strain BV410 was optimized to yield 36.94 mg/l after 14 days of incubation in soy flowerglucose- starch-mannitol based fermentation medium (JS). Further optimization of medium for biosynthesis of staurosporine indicated the following optimal values of the examined factors: the content of glucose of 20 g/l, starch 0.36 g/l, mannitol 21.46 g/l, soy flower 17.32 g/l. By applying the defined optimal values and using the appropriate mathematical models, the following responses were predicted: concentration of staurosporine 46.88 mg/l and biomass yield 12.05 mg/ml. The validity of the results was confirmed by performing the biosynthesis of the staurosporine in the medium with optimal composition (JSSta). Kinetics of staurosporine and biomass production and carbon source consumption were examined and process models were developed. Additionally, optimization of staurosporine production was performed with different supplements which, according to literature data, had stimulative effect on secondary metabolism (Zn, Fe and P salts, methyl oleate, grape seed oil). In order to improve the production of staurosporine, effects of pH (6.5, 7.5 and 8.5) and incubation time (7, 10 and 14 days) were also examined. It was found that addition of FeS04 significantly improved the staurosporine yield in comparison to the starting conditions (increase of 25%). Our results proved that rhizosphere soils of ethno-medicinal plants are a prolific source of streptomycetes, producers of compounds with good antifungal activity. Isolation of the new staurosporine producing strain, allowed for its detailed bioactivity assessment. Staurosporine scaffold might serve as a lead structure for the development of new antifungal and antiangiogenic agents. Also, results obtained within this research represent the basis for the further scale-up and potential industrialization of the proposed production process.",
publisher = "Универзитет у Новом Саду, Технолошки факултет",
journal = "Универзитет у Новом Саду",
title = "Antifungalni potencijal streptomiceta izolovanih iz rizosfera medicinski značajnih biljaka: karakterizacija i optimizacija biosinteze staurosporina, produkta metabolizma Streptomyces sp. BV410",
url = "https://hdl.handle.net/21.15107/rcub_nardus_11581"
}

Mojićević, M.. (2019-12-13). Antifungalni potencijal streptomiceta izolovanih iz rizosfera medicinski značajnih biljaka: karakterizacija i optimizacija biosinteze staurosporina, produkta metabolizma Streptomyces sp. BV410. in Универзитет у Новом Саду
Универзитет у Новом Саду, Технолошки факултет..
https://hdl.handle.net/21.15107/rcub_nardus_11581

Mojićević M. Antifungalni potencijal streptomiceta izolovanih iz rizosfera medicinski značajnih biljaka: karakterizacija i optimizacija biosinteze staurosporina, produkta metabolizma Streptomyces sp. BV410. in Универзитет у Новом Саду. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_11581 .

Mojićević, Marija, "Antifungalni potencijal streptomiceta izolovanih iz rizosfera medicinski značajnih biljaka: karakterizacija i optimizacija biosinteze staurosporina, produkta metabolizma Streptomyces sp. BV410" in Универзитет у Новом Саду (2019-12-13),
https://hdl.handle.net/21.15107/rcub_nardus_11581 .

TY  - JOUR
AU  - Jovanović, Milos
AU  - Milovanović, Jelena
AU  - O'Connor, Kevin
AU  - Blagojević, Stevan
AU  - Begović, Biljana 
AU  - Lukić, Vera
AU  - Nikodinović-Runić, Jasmina
AU  - Savić, Vladimir
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1258
AB  - Rhamnolipids are biodegradable low toxic biosurfactants which exert antimicrobial and anti-biofilm properties. They have attracted much attention recently due to potential applications in areas of bioremediation, therapeutics, cosmetics and agriculture, however, the full potential of these versatile molecules is yet to be explored. Based on the facts that many naturally occurring lipopeptides are potent antimicrobials, our study aimed to explore the potential of replacing rhamnose in rhamnolipids with amino acids thus creating lipopeptides that would mimic or enhance properties of the parent molecule. This would allow not only for more economical and greener production but also, due to the availability of structurally different amino acids, facile manipulation of physico-chemical and biological properties. Our synthetic efforts produced a library of 43 lipopeptides revealing biologically more potent molecules. The structural changes significantly increased, in particular, anti-biofilm properties against Candida albicans, although surface activity of the parent molecule was almost completely abolished. Our findings show that the most active compounds are leucine derivatives of 3-hydroxy acids containing benzylic ester functionality. The SAR study demonstrated a further increase in activity with aliphatic chain elongation. The most promising lipopeptides 15, 23 and 36 at 12.5 mu g/mL concentration allowed only 14.3%, 5.1% and 11.2% of biofilm formation, respectively after 24 h. These compounds inhibit biofilm formation by preventing adhesion of C. albicans to abiotic and biotic surfaces.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Bioorganic Chemistry
T1  - Rhamnolipid inspired lipopeptides effective in preventing adhesion and biofilm formation of Candida albicans
EP  - 217
SP  - 209
VL  - 87
DO  - 10.1016/j.bioorg.2019.03.023
ER  - 

@article{
author = "Jovanović, Milos and Milovanović, Jelena and O'Connor, Kevin and Blagojević, Stevan and Begović, Biljana  and Lukić, Vera and Nikodinović-Runić, Jasmina and Savić, Vladimir",
year = "2019",
abstract = "Rhamnolipids are biodegradable low toxic biosurfactants which exert antimicrobial and anti-biofilm properties. They have attracted much attention recently due to potential applications in areas of bioremediation, therapeutics, cosmetics and agriculture, however, the full potential of these versatile molecules is yet to be explored. Based on the facts that many naturally occurring lipopeptides are potent antimicrobials, our study aimed to explore the potential of replacing rhamnose in rhamnolipids with amino acids thus creating lipopeptides that would mimic or enhance properties of the parent molecule. This would allow not only for more economical and greener production but also, due to the availability of structurally different amino acids, facile manipulation of physico-chemical and biological properties. Our synthetic efforts produced a library of 43 lipopeptides revealing biologically more potent molecules. The structural changes significantly increased, in particular, anti-biofilm properties against Candida albicans, although surface activity of the parent molecule was almost completely abolished. Our findings show that the most active compounds are leucine derivatives of 3-hydroxy acids containing benzylic ester functionality. The SAR study demonstrated a further increase in activity with aliphatic chain elongation. The most promising lipopeptides 15, 23 and 36 at 12.5 mu g/mL concentration allowed only 14.3%, 5.1% and 11.2% of biofilm formation, respectively after 24 h. These compounds inhibit biofilm formation by preventing adhesion of C. albicans to abiotic and biotic surfaces.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Bioorganic Chemistry",
title = "Rhamnolipid inspired lipopeptides effective in preventing adhesion and biofilm formation of Candida albicans",
pages = "217-209",
volume = "87",
doi = "10.1016/j.bioorg.2019.03.023"
}

Jovanović, M., Milovanović, J., O'Connor, K., Blagojević, S., Begović, B., Lukić, V., Nikodinović-Runić, J.,& Savić, V.. (2019). Rhamnolipid inspired lipopeptides effective in preventing adhesion and biofilm formation of Candida albicans. in Bioorganic Chemistry
Academic Press Inc Elsevier Science, San Diego., 87, 209-217.
https://doi.org/10.1016/j.bioorg.2019.03.023

Jovanović M, Milovanović J, O'Connor K, Blagojević S, Begović B, Lukić V, Nikodinović-Runić J, Savić V. Rhamnolipid inspired lipopeptides effective in preventing adhesion and biofilm formation of Candida albicans. in Bioorganic Chemistry. 2019;87:209-217.
doi:10.1016/j.bioorg.2019.03.023 .

Jovanović, Milos, Milovanović, Jelena, O'Connor, Kevin, Blagojević, Stevan, Begović, Biljana , Lukić, Vera, Nikodinović-Runić, Jasmina, Savić, Vladimir, "Rhamnolipid inspired lipopeptides effective in preventing adhesion and biofilm formation of Candida albicans" in Bioorganic Chemistry, 87 (2019):209-217,
https://doi.org/10.1016/j.bioorg.2019.03.023 . .

TY  - JOUR
AU  - Stevanović, Nevena Lj.
AU  - Andrejević, Tina P.
AU  - Crochet, Aurelien
AU  - Ilić-Tomić, Tatjana
AU  - Drasković, Nenad S.
AU  - Nikodinović-Runić, Jasmina
AU  - Fromm, Katharina M.
AU  - Djuran, Milos 
AU  - Glišić, Biljana
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1222
AB  - The reactions between equimolar amounts of CuX2 (X = NO3- and CF3SO3-) and two aromatic nitrogen-containing heterocycles differing in the position of nitrogen atoms, 1,7- and 4,7-phenanthroline (1,7-and 4,7-phen), were performed in ethanol/methanol at room temperature. When CuX 2 salts were mixed with 4,7-phen, two copper(II) complexes, [Cu(NO3)(2)(4,7-Hphen)(2)](NO3)(2) (1) and [Cu(CF3SO3)(4,7-phen)(2)(H2O)(2)]CF3SO3 (2), were formed. On the other hand, in the reaction of CuX2 salts with 1,7-phen, only 1,7-HphenNO(3) (3a/b) and 1,7-HphenCF(3)SO(3) (4) were obtained as the final products. The obtained products 1-4 were characterized by spectroscopic and X-ray diffraction techniques. In the copper(II) complexes 1 and 2, the coordination geometry around the Cu(II) ion is distorted octahedral and square pyramidal, respectively. The antimicrobial potential of the copper(II) complexes 1 and 2 and corresponding compounds used for their synthesis were assessed against four different bacterial species and Candida albicans, displaying moderate growth inhibiting activity. The cytotoxic properties of the investigated complexes were also evaluated against the normal human lung fibroblast cell line (MRC-5) indicating moderate, yet more pronounced cytotoxicity than antimicrobial properties.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Polyhedron
T1  - Different coordination abilities of 1,7-and 4,7-phenanthroline in the reactions with copper(II) salts: Structural characterization and biological evaluation of the reaction products
VL  - 173
DO  - 10.1016/j.poly.2019.114112
ER  - 

@article{
author = "Stevanović, Nevena Lj. and Andrejević, Tina P. and Crochet, Aurelien and Ilić-Tomić, Tatjana and Drasković, Nenad S. and Nikodinović-Runić, Jasmina and Fromm, Katharina M. and Djuran, Milos  and Glišić, Biljana",
year = "2019",
abstract = "The reactions between equimolar amounts of CuX2 (X = NO3- and CF3SO3-) and two aromatic nitrogen-containing heterocycles differing in the position of nitrogen atoms, 1,7- and 4,7-phenanthroline (1,7-and 4,7-phen), were performed in ethanol/methanol at room temperature. When CuX 2 salts were mixed with 4,7-phen, two copper(II) complexes, [Cu(NO3)(2)(4,7-Hphen)(2)](NO3)(2) (1) and [Cu(CF3SO3)(4,7-phen)(2)(H2O)(2)]CF3SO3 (2), were formed. On the other hand, in the reaction of CuX2 salts with 1,7-phen, only 1,7-HphenNO(3) (3a/b) and 1,7-HphenCF(3)SO(3) (4) were obtained as the final products. The obtained products 1-4 were characterized by spectroscopic and X-ray diffraction techniques. In the copper(II) complexes 1 and 2, the coordination geometry around the Cu(II) ion is distorted octahedral and square pyramidal, respectively. The antimicrobial potential of the copper(II) complexes 1 and 2 and corresponding compounds used for their synthesis were assessed against four different bacterial species and Candida albicans, displaying moderate growth inhibiting activity. The cytotoxic properties of the investigated complexes were also evaluated against the normal human lung fibroblast cell line (MRC-5) indicating moderate, yet more pronounced cytotoxicity than antimicrobial properties.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Polyhedron",
title = "Different coordination abilities of 1,7-and 4,7-phenanthroline in the reactions with copper(II) salts: Structural characterization and biological evaluation of the reaction products",
volume = "173",
doi = "10.1016/j.poly.2019.114112"
}

Stevanović, N. Lj., Andrejević, T. P., Crochet, A., Ilić-Tomić, T., Drasković, N. S., Nikodinović-Runić, J., Fromm, K. M., Djuran, M.,& Glišić, B.. (2019). Different coordination abilities of 1,7-and 4,7-phenanthroline in the reactions with copper(II) salts: Structural characterization and biological evaluation of the reaction products. in Polyhedron
Pergamon-Elsevier Science Ltd, Oxford., 173.
https://doi.org/10.1016/j.poly.2019.114112

Stevanović NL, Andrejević TP, Crochet A, Ilić-Tomić T, Drasković NS, Nikodinović-Runić J, Fromm KM, Djuran M, Glišić B. Different coordination abilities of 1,7-and 4,7-phenanthroline in the reactions with copper(II) salts: Structural characterization and biological evaluation of the reaction products. in Polyhedron. 2019;173.
doi:10.1016/j.poly.2019.114112 .

Stevanović, Nevena Lj., Andrejević, Tina P., Crochet, Aurelien, Ilić-Tomić, Tatjana, Drasković, Nenad S., Nikodinović-Runić, Jasmina, Fromm, Katharina M., Djuran, Milos , Glišić, Biljana, "Different coordination abilities of 1,7-and 4,7-phenanthroline in the reactions with copper(II) salts: Structural characterization and biological evaluation of the reaction products" in Polyhedron, 173 (2019),
https://doi.org/10.1016/j.poly.2019.114112 . .