TY  - JOUR
AU  - Makryniotis, Konstantinos
AU  - Nikolaivits, Efstratios
AU  - Taxeidis, George
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.202400053
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2345
AB  - The rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering recycling process challenging. The elucidation of the selective degradation of polyesters by hydrolases could offer a proper solution to this problem, enhancing the recyclability performance. Towards this, our study focused on the investigation of four bacterial polyesterases, including DaPUase, IsPETase, PfPHOase, and Se1JFR, a novel PETase-like lipase. The enzymes, which were biochemically characterized and structurally analyzed, demonstrated degradation ability of synthetic plastics. While a consistent pattern of polyesters’ degradation was observed across all enzymes, Se1JFR stood out in the degradation of PBS, PLA, and polyether PU. Additionally, it exhibited comparable results to IsPETase, a benchmark mesophilic PETase, in the degradation of PCL and semi-crystalline PET. Our results point out the wide substrate spectrum of bacterial hydrolases and underscore the significant potential of PETase-like enzymes in polyesters degradation.
T2  - Biotechnology Journal
T1  - Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases
IS  - n/a
SP  - 2400053
VL  - n/a
DO  - 10.1002/biot.202400053
ER  - 

@article{
author = "Makryniotis, Konstantinos and Nikolaivits, Efstratios and Taxeidis, George and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
abstract = "The rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering recycling process challenging. The elucidation of the selective degradation of polyesters by hydrolases could offer a proper solution to this problem, enhancing the recyclability performance. Towards this, our study focused on the investigation of four bacterial polyesterases, including DaPUase, IsPETase, PfPHOase, and Se1JFR, a novel PETase-like lipase. The enzymes, which were biochemically characterized and structurally analyzed, demonstrated degradation ability of synthetic plastics. While a consistent pattern of polyesters’ degradation was observed across all enzymes, Se1JFR stood out in the degradation of PBS, PLA, and polyether PU. Additionally, it exhibited comparable results to IsPETase, a benchmark mesophilic PETase, in the degradation of PCL and semi-crystalline PET. Our results point out the wide substrate spectrum of bacterial hydrolases and underscore the significant potential of PETase-like enzymes in polyesters degradation.",
journal = "Biotechnology Journal",
title = "Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases",
number = "n/a",
pages = "2400053",
volume = "n/a",
doi = "10.1002/biot.202400053"
}

Makryniotis, K., Nikolaivits, E., Taxeidis, G., Nikodinović-Runić, J.,& Topakas, E..Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases. in Biotechnology Journal, n/a(n/a), 2400053.
https://doi.org/10.1002/biot.202400053

Makryniotis K, Nikolaivits E, Taxeidis G, Nikodinović-Runić J, Topakas E. Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases. in Biotechnology Journal.n/a(n/a):2400053.
doi:10.1002/biot.202400053 .

Makryniotis, Konstantinos, Nikolaivits, Efstratios, Taxeidis, George, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases" in Biotechnology Journal, n/a, no. n/a:2400053,
https://doi.org/10.1002/biot.202400053 . .

TY  - JOUR
AU  - Makryniotis, Konstantinos
AU  - Nikolaivits, Efstratios
AU  - Taxeidis, George
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.202400053
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2341
AB  - The rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering recycling process challenging. The elucidation of the selective degradation of polyesters by hydrolases could offer a proper solution to this problem, enhancing the recyclability performance. Towards this, our study focused on the investigation of four bacterial polyesterases, including DaPUase, IsPETase, PfPHOase, and Se1JFR, a novel PETase-like lipase. The enzymes, which were biochemically characterized and structurally analyzed, demonstrated degradation ability of synthetic plastics. While a consistent pattern of polyesters’ degradation was observed across all enzymes, Se1JFR stood out in the degradation of PBS, PLA, and polyether PU. Additionally, it exhibited comparable results to IsPETase, a benchmark mesophilic PETase, in the degradation of PCL and semi-crystalline PET. Our results point out the wide substrate spectrum of bacterial hydrolases and underscore the significant potential of PETase-like enzymes in polyesters degradation.
T2  - Biotechnology Journal
T2  - Biotechnology Journal
T1  - Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases
IS  - n/a
SP  - 2400053
VL  - n/a
DO  - 10.1002/biot.202400053
ER  - 

@article{
author = "Makryniotis, Konstantinos and Nikolaivits, Efstratios and Taxeidis, George and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
abstract = "The rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering recycling process challenging. The elucidation of the selective degradation of polyesters by hydrolases could offer a proper solution to this problem, enhancing the recyclability performance. Towards this, our study focused on the investigation of four bacterial polyesterases, including DaPUase, IsPETase, PfPHOase, and Se1JFR, a novel PETase-like lipase. The enzymes, which were biochemically characterized and structurally analyzed, demonstrated degradation ability of synthetic plastics. While a consistent pattern of polyesters’ degradation was observed across all enzymes, Se1JFR stood out in the degradation of PBS, PLA, and polyether PU. Additionally, it exhibited comparable results to IsPETase, a benchmark mesophilic PETase, in the degradation of PCL and semi-crystalline PET. Our results point out the wide substrate spectrum of bacterial hydrolases and underscore the significant potential of PETase-like enzymes in polyesters degradation.",
journal = "Biotechnology Journal, Biotechnology Journal",
title = "Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases",
number = "n/a",
pages = "2400053",
volume = "n/a",
doi = "10.1002/biot.202400053"
}

Makryniotis, K., Nikolaivits, E., Taxeidis, G., Nikodinović-Runić, J.,& Topakas, E..Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases. in Biotechnology Journal, n/a(n/a), 2400053.
https://doi.org/10.1002/biot.202400053

Makryniotis K, Nikolaivits E, Taxeidis G, Nikodinović-Runić J, Topakas E. Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases. in Biotechnology Journal.n/a(n/a):2400053.
doi:10.1002/biot.202400053 .

Makryniotis, Konstantinos, Nikolaivits, Efstratios, Taxeidis, George, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases" in Biotechnology Journal, n/a, no. n/a:2400053,
https://doi.org/10.1002/biot.202400053 . .

TY  - JOUR
AU  - Karagüzel, Ayşe
AU  - Uğur, Sümeyye Buran
AU  - Çetinkaya, Yasin
AU  - Doğan, Şengül Dilem
AU  - Stevanović, Milena
AU  - Nikodinović-Runić, Jasmina
AU  - Gündüz, Miyase Gözde
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0022286024003107
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2317
AB  - Nonsteroidal anti-inflammatory drugs (NSAIDs) alleviate inflammation and pain through the inhibition of cyclooxygenase (COX) enzymes. Besides these widely recognized therapeutic utilizations, NSAIDs have been reported to display moderate antimicrobial activity and enhance antimicrobial efficacy when administered in combination with commercial antimicrobial drugs. In the present study, we designed novel potential antimicrobial agents by linking some NSAIDs (ibuprofen, flurbiprofen, and naproxen) to various azole rings (pyrazole, imidazole, triazole, and benzimidazole) via hydrazone functionality. The hydrazone linker was introduced into the chemical scaffold of the title molecules by the reaction between hydrazides obtained from NSAIDs and in-house synthesized azole-carrying benzaldehydes. The structures of the target compounds were elucidated by a combination of spectral methods. The NOESY spectra and stereochemical analyses performed using DFT method confirmed the presence of the target molecules as a mixture of E(C=N)-E(N-N)-synperiplanar and E(C=N)-E(N-N)-antiperiplanar conformers in DMSO-d6 solution. 1H and 13C NMR chemical shift values in DMSO were calculated using the GIAO method and compared with the experimental NMR data. Finally, some derivatives were demonstrated to inhibit Candida albicans filamentation and/or bacterial communication system known as quorum sensing. For COX inhibitor-azole hybrids with antimicrobial potency, naproxen appeared to be the most appropriate NSAID, while bulky benzimidazole was not found as a preferable azole ring.
PB  - Elsevier
T2  - Journal of Molecular Structure
T2  - Journal of Molecular StructureJournal of Molecular Structure
T1  - Azole rings linked to COX inhibitors via hydrazone bridge: Synthesis, stereochemical analysis, and investigation of antimicrobial activity
SP  - 137787
DO  - 10.1016/j.molstruc.2024.137787
ER  - 

@article{
author = "Karagüzel, Ayşe and Uğur, Sümeyye Buran and Çetinkaya, Yasin and Doğan, Şengül Dilem and Stevanović, Milena and Nikodinović-Runić, Jasmina and Gündüz, Miyase Gözde",
year = "2024",
abstract = "Nonsteroidal anti-inflammatory drugs (NSAIDs) alleviate inflammation and pain through the inhibition of cyclooxygenase (COX) enzymes. Besides these widely recognized therapeutic utilizations, NSAIDs have been reported to display moderate antimicrobial activity and enhance antimicrobial efficacy when administered in combination with commercial antimicrobial drugs. In the present study, we designed novel potential antimicrobial agents by linking some NSAIDs (ibuprofen, flurbiprofen, and naproxen) to various azole rings (pyrazole, imidazole, triazole, and benzimidazole) via hydrazone functionality. The hydrazone linker was introduced into the chemical scaffold of the title molecules by the reaction between hydrazides obtained from NSAIDs and in-house synthesized azole-carrying benzaldehydes. The structures of the target compounds were elucidated by a combination of spectral methods. The NOESY spectra and stereochemical analyses performed using DFT method confirmed the presence of the target molecules as a mixture of E(C=N)-E(N-N)-synperiplanar and E(C=N)-E(N-N)-antiperiplanar conformers in DMSO-d6 solution. 1H and 13C NMR chemical shift values in DMSO were calculated using the GIAO method and compared with the experimental NMR data. Finally, some derivatives were demonstrated to inhibit Candida albicans filamentation and/or bacterial communication system known as quorum sensing. For COX inhibitor-azole hybrids with antimicrobial potency, naproxen appeared to be the most appropriate NSAID, while bulky benzimidazole was not found as a preferable azole ring.",
publisher = "Elsevier",
journal = "Journal of Molecular Structure, Journal of Molecular StructureJournal of Molecular Structure",
title = "Azole rings linked to COX inhibitors via hydrazone bridge: Synthesis, stereochemical analysis, and investigation of antimicrobial activity",
pages = "137787",
doi = "10.1016/j.molstruc.2024.137787"
}

Karagüzel, A., Uğur, S. B., Çetinkaya, Y., Doğan, Ş. D., Stevanović, M., Nikodinović-Runić, J.,& Gündüz, M. G.. (2024). Azole rings linked to COX inhibitors via hydrazone bridge: Synthesis, stereochemical analysis, and investigation of antimicrobial activity. in Journal of Molecular Structure
Elsevier., 137787.
https://doi.org/10.1016/j.molstruc.2024.137787

Karagüzel A, Uğur SB, Çetinkaya Y, Doğan ŞD, Stevanović M, Nikodinović-Runić J, Gündüz MG. Azole rings linked to COX inhibitors via hydrazone bridge: Synthesis, stereochemical analysis, and investigation of antimicrobial activity. in Journal of Molecular Structure. 2024;:137787.
doi:10.1016/j.molstruc.2024.137787 .

Karagüzel, Ayşe, Uğur, Sümeyye Buran, Çetinkaya, Yasin, Doğan, Şengül Dilem, Stevanović, Milena, Nikodinović-Runić, Jasmina, Gündüz, Miyase Gözde, "Azole rings linked to COX inhibitors via hydrazone bridge: Synthesis, stereochemical analysis, and investigation of antimicrobial activity" in Journal of Molecular Structure (2024):137787,
https://doi.org/10.1016/j.molstruc.2024.137787 . .

TY  - JOUR
AU  - Balint, Vanda
AU  - Perić, Mina
AU  - Dačić, Sanja
AU  - Stanisavljević Ninković, Danijela
AU  - Marjanović, Jelena
AU  - Popović, Jelena
AU  - Stevanović, Milena
AU  - Lazić, Andrijana
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2340
AB  - Astrocytes are the main homeostatic cells in the central nervous system, with the unique ability to transform from quiescent into a reactive state in response to pathological conditions by reacquiring some precursor properties. This process is known as reactive astrogliosis, a compensatory response that mediates tissue damage and recovery. Although it is well known that SOX transcription factors drive the expression of phenotype-specific genetic programs during neurodevelopment, their roles in mature astrocytes have not been studied extensively. We focused on the transcription factors SOX2 and SOX9, shown to be re-expressed in reactive astrocytes, in order to study the reactivation-related functional properties of astrocytes mediated by those proteins. We performed an initial screening of SOX2 and SOX9 expression after sensorimotor cortex ablation injury in rats and conducted gain-of-function studies in vitro using astrocytes derived from the human NT2/D1 cell line. Our results revealed the direct involvement of SOX2 in the reacquisition of proliferation in mature NT2/D1-derived astrocytes, while SOX9 overexpression increased migratory potential and glutamate uptake in these cells. Our results imply that modulation of SOX gene expression may change the functional properties of astrocytes, which holds promise for the discovery of potential therapeutic targets in the development of novel strategies for tissue regeneration and recovery.
PB  - MDPI
T2  - Biomedicines
T1  - The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes
IS  - 4
SP  - 796
VL  - 12
DO  - 10.3390/biomedicines12040796
ER  - 

@article{
author = "Balint, Vanda and Perić, Mina and Dačić, Sanja and Stanisavljević Ninković, Danijela and Marjanović, Jelena and Popović, Jelena and Stevanović, Milena and Lazić, Andrijana",
year = "2024",
abstract = "Astrocytes are the main homeostatic cells in the central nervous system, with the unique ability to transform from quiescent into a reactive state in response to pathological conditions by reacquiring some precursor properties. This process is known as reactive astrogliosis, a compensatory response that mediates tissue damage and recovery. Although it is well known that SOX transcription factors drive the expression of phenotype-specific genetic programs during neurodevelopment, their roles in mature astrocytes have not been studied extensively. We focused on the transcription factors SOX2 and SOX9, shown to be re-expressed in reactive astrocytes, in order to study the reactivation-related functional properties of astrocytes mediated by those proteins. We performed an initial screening of SOX2 and SOX9 expression after sensorimotor cortex ablation injury in rats and conducted gain-of-function studies in vitro using astrocytes derived from the human NT2/D1 cell line. Our results revealed the direct involvement of SOX2 in the reacquisition of proliferation in mature NT2/D1-derived astrocytes, while SOX9 overexpression increased migratory potential and glutamate uptake in these cells. Our results imply that modulation of SOX gene expression may change the functional properties of astrocytes, which holds promise for the discovery of potential therapeutic targets in the development of novel strategies for tissue regeneration and recovery.",
publisher = "MDPI",
journal = "Biomedicines",
title = "The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes",
number = "4",
pages = "796",
volume = "12",
doi = "10.3390/biomedicines12040796"
}

Balint, V., Perić, M., Dačić, S., Stanisavljević Ninković, D., Marjanović, J., Popović, J., Stevanović, M.,& Lazić, A.. (2024). The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes. in Biomedicines
MDPI., 12(4), 796.
https://doi.org/10.3390/biomedicines12040796

Balint V, Perić M, Dačić S, Stanisavljević Ninković D, Marjanović J, Popović J, Stevanović M, Lazić A. The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes. in Biomedicines. 2024;12(4):796.
doi:10.3390/biomedicines12040796 .

Balint, Vanda, Perić, Mina, Dačić, Sanja, Stanisavljević Ninković, Danijela, Marjanović, Jelena, Popović, Jelena, Stevanović, Milena, Lazić, Andrijana, "The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes" in Biomedicines, 12, no. 4 (2024):796,
https://doi.org/10.3390/biomedicines12040796 . .

TY  - JOUR
AU  - Rakonjac, Marijana
AU  - Cuturilo, Goran
AU  - Kovačević-Grujičić, Nataša
AU  - Simeunović, Ivana
AU  - Kostić, Jovana
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
PY  - 2024
UR  - https://www.mdpi.com/2227-9067/11/4/489
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2362
AB  - 22q11.2 deletion syndrome (22q11.2DS), the most frequent microdeletion syndrome in humans, is related to a high risk of developing neurodevelopmental disorders. About 95% of patients with 22q11.2DS have speech and language impairments. Global articulation, story generation, and verbal memory tests were applied to compare articulatory characteristics of speech sounds, spontaneous language abilities, and immediate verbal memory between four groups of Serbian-speaking children: patients with 22q11.2DS, children with clinical presentation of 22q11.2DS that do not have the microdeletion, children with non-syndromic congenital heart defects, and their peers with typical speech–sound development. The obtained results showed that children with this microdeletion have impaired articulation skills and expressive language abilities. However, we did not observe weaker receptive language skills and immediate verbal memory compared to healthy controls. Children with 22q11.2DS should be considered a risk category for the development of speech–sound pathology and expressive language abilities. Since speech intelligibility is an instrument of cognition and adequate peer socialization, and language impairment in school-aged children with 22q11DS might be an indicator of increased risk for later psychotic symptoms, patients with 22q11.2 microdeletion should be included in a program of early stimulation of speech–language development immediately after diagnosis is established.
PB  - MDPI
T2  - Children
T2  - Children
T1  - Speech Sounds Production, Narrative Skills, and Verbal Memory of Children with 22q11.2 Microdeletion
IS  - 4
SP  - 489
VL  - 11
DO  - 10.3390/children11040489
ER  - 

@article{
author = "Rakonjac, Marijana and Cuturilo, Goran and Kovačević-Grujičić, Nataša and Simeunović, Ivana and Kostić, Jovana and Stevanović, Milena and Drakulić, Danijela",
year = "2024",
abstract = "22q11.2 deletion syndrome (22q11.2DS), the most frequent microdeletion syndrome in humans, is related to a high risk of developing neurodevelopmental disorders. About 95% of patients with 22q11.2DS have speech and language impairments. Global articulation, story generation, and verbal memory tests were applied to compare articulatory characteristics of speech sounds, spontaneous language abilities, and immediate verbal memory between four groups of Serbian-speaking children: patients with 22q11.2DS, children with clinical presentation of 22q11.2DS that do not have the microdeletion, children with non-syndromic congenital heart defects, and their peers with typical speech–sound development. The obtained results showed that children with this microdeletion have impaired articulation skills and expressive language abilities. However, we did not observe weaker receptive language skills and immediate verbal memory compared to healthy controls. Children with 22q11.2DS should be considered a risk category for the development of speech–sound pathology and expressive language abilities. Since speech intelligibility is an instrument of cognition and adequate peer socialization, and language impairment in school-aged children with 22q11DS might be an indicator of increased risk for later psychotic symptoms, patients with 22q11.2 microdeletion should be included in a program of early stimulation of speech–language development immediately after diagnosis is established.",
publisher = "MDPI",
journal = "Children, Children",
title = "Speech Sounds Production, Narrative Skills, and Verbal Memory of Children with 22q11.2 Microdeletion",
number = "4",
pages = "489",
volume = "11",
doi = "10.3390/children11040489"
}

Rakonjac, M., Cuturilo, G., Kovačević-Grujičić, N., Simeunović, I., Kostić, J., Stevanović, M.,& Drakulić, D.. (2024). Speech Sounds Production, Narrative Skills, and Verbal Memory of Children with 22q11.2 Microdeletion. in Children
MDPI., 11(4), 489.
https://doi.org/10.3390/children11040489

Rakonjac M, Cuturilo G, Kovačević-Grujičić N, Simeunović I, Kostić J, Stevanović M, Drakulić D. Speech Sounds Production, Narrative Skills, and Verbal Memory of Children with 22q11.2 Microdeletion. in Children. 2024;11(4):489.
doi:10.3390/children11040489 .

Rakonjac, Marijana, Cuturilo, Goran, Kovačević-Grujičić, Nataša, Simeunović, Ivana, Kostić, Jovana, Stevanović, Milena, Drakulić, Danijela, "Speech Sounds Production, Narrative Skills, and Verbal Memory of Children with 22q11.2 Microdeletion" in Children, 11, no. 4 (2024):489,
https://doi.org/10.3390/children11040489 . .

TY  - GEN
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2289
AB  - GlucoAdjust project aims to solve fast-growing health challenges important for the society,
namely it will focus on discovery of new treatments for one rare disease glycogen storage
disease type Ib (GSD Ib) and one common disease diabetes mellitus type 2 (DM type 2). We
propose a completely new concept based on the identification of small molecule (SM)
compounds able to directly bind to glucose-6-phospate translocase (G6PT) and thus fine-tune
glucose level. To tackle these challenges, an interdisciplinary international team will screen large
library of SMs combining in silico and in vitro approaches and identify SMs that directly bind to
G6PT. SMs able to stabilize G6PT and increase its function thus correcting hypoglycemia are
potential drugs for GSD Ib. On the other hand, SMs that inhibit G6PT may be used to induce
hypoglycemia in DM type 2 treatment. To obtain highly functional results of testing SMs in vitro,
human hepatocyte models for GSD Ib and DM type 2 as well as controls will be developed
(differentiated from human healthy and GSD Ib iPSC and diabetic adipose stem cells). For the
first time, whole transcriptome of human GSD Ib hepatocytes will be used to delineate molecular
processes disturbed due to G6PT deficiency. As a result, RNA hallmarks of GSD Ib phenotype will
be determined and used for evaluation of SMs’ effect in both models. To be efficient for GSD Ib,
SMs will have to revert GSD Ib phenotype into normal. The key to discover satisfactorily
effective, yet sufficiently mild inhibitor for DM type 2 will be to avoid hallmarks representing
GSD Ib phenotype. Thus, a revolutionary concept of using GSD Ib as a model of hypoglycemia to
better optimize DM type 2 treatment is proposed here. Results will be openly disseminated to
make a wide scientific, educational, social and economic impacts. GlucoAdjust anticipates
innovative results with a potential to be further translated into drugs able to improve lives of
people with GSD Ib and DM type 2 worldwide.
T2  - Program PRISMA
T1  - New concept for treatment of glycogen storage disease Ib and diabetes mellitus type 2: small molecule compounds able to adjust glucose level through binding glucose-6-phospate translocase (GlucoAdjust)
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2289
ER  - 

@misc{
year = "2024",
abstract = "GlucoAdjust project aims to solve fast-growing health challenges important for the society,
namely it will focus on discovery of new treatments for one rare disease glycogen storage
disease type Ib (GSD Ib) and one common disease diabetes mellitus type 2 (DM type 2). We
propose a completely new concept based on the identification of small molecule (SM)
compounds able to directly bind to glucose-6-phospate translocase (G6PT) and thus fine-tune
glucose level. To tackle these challenges, an interdisciplinary international team will screen large
library of SMs combining in silico and in vitro approaches and identify SMs that directly bind to
G6PT. SMs able to stabilize G6PT and increase its function thus correcting hypoglycemia are
potential drugs for GSD Ib. On the other hand, SMs that inhibit G6PT may be used to induce
hypoglycemia in DM type 2 treatment. To obtain highly functional results of testing SMs in vitro,
human hepatocyte models for GSD Ib and DM type 2 as well as controls will be developed
(differentiated from human healthy and GSD Ib iPSC and diabetic adipose stem cells). For the
first time, whole transcriptome of human GSD Ib hepatocytes will be used to delineate molecular
processes disturbed due to G6PT deficiency. As a result, RNA hallmarks of GSD Ib phenotype will
be determined and used for evaluation of SMs’ effect in both models. To be efficient for GSD Ib,
SMs will have to revert GSD Ib phenotype into normal. The key to discover satisfactorily
effective, yet sufficiently mild inhibitor for DM type 2 will be to avoid hallmarks representing
GSD Ib phenotype. Thus, a revolutionary concept of using GSD Ib as a model of hypoglycemia to
better optimize DM type 2 treatment is proposed here. Results will be openly disseminated to
make a wide scientific, educational, social and economic impacts. GlucoAdjust anticipates
innovative results with a potential to be further translated into drugs able to improve lives of
people with GSD Ib and DM type 2 worldwide.",
journal = "Program PRISMA",
title = "New concept for treatment of glycogen storage disease Ib and diabetes mellitus type 2: small molecule compounds able to adjust glucose level through binding glucose-6-phospate translocase (GlucoAdjust)",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2289"
}

(2024). New concept for treatment of glycogen storage disease Ib and diabetes mellitus type 2: small molecule compounds able to adjust glucose level through binding glucose-6-phospate translocase (GlucoAdjust). in Program PRISMA.
https://hdl.handle.net/21.15107/rcub_imagine_2289

New concept for treatment of glycogen storage disease Ib and diabetes mellitus type 2: small molecule compounds able to adjust glucose level through binding glucose-6-phospate translocase (GlucoAdjust). in Program PRISMA. 2024;.
https://hdl.handle.net/21.15107/rcub_imagine_2289 .

"New concept for treatment of glycogen storage disease Ib and diabetes mellitus type 2: small molecule compounds able to adjust glucose level through binding glucose-6-phospate translocase (GlucoAdjust)" in Program PRISMA (2024),
https://hdl.handle.net/21.15107/rcub_imagine_2289 .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Ušjak, Dušan
AU  - Rambaher, Martina Hrast
AU  - Oljacic, Slavica
AU  - Milenković, Marina
PY  - 2024
UR  - https://www.frontiersin.org/articles/10.3389/fcimb.2024.1370062
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2333
AB  - Antimicrobial resistance is a global threat, leading to an alarming increase in the prevalence of bacterial infections that can no longer be treated with available antibiotics. The World Health Organization estimates that by 2050 up to 10 million deaths per year could be associated with antimicrobial resistance, which would equal the annual number of cancer deaths worldwide. To overcome this emerging crisis, novel anti-bacterial compounds are urgently needed. There are two possible approaches in the fight against bacterial infections: a) targeting structures within bacterial cells, similar to existing antibiotics; and/or b) targeting virulence factors rather than bacterial growth. Here, for the first time, we provide a comprehensive overview of the key steps in the evaluation of potential new anti-bacterial and/or anti-virulence compounds. The methods described in this review include: a) in silico methods for the evaluation of novel compounds; b) anti-bacterial assays (MIC, MBC, Time-kill); b) anti-virulence assays (anti-biofilm, anti-quorum sensing, anti-adhesion); and c) evaluation of safety aspects (cytotoxicity assay and Ames test). Overall, we provide a detailed description of the methods that are an essential tool for chemists, computational chemists, microbiologists, and toxicologists in the evaluation of potential novel antimicrobial compounds. These methods are cost-effective and have high predictive value. They are widely used in preclinical studies to identify new molecular candidates, for further investigation in animal and human trials.
PB  - Frontiers
T2  - Frontiers in Cellular and Infection Microbiology
T1  - Evaluation of novel compounds as anti-bacterial or anti-virulence agents
VL  - 14
DO  - 10.3389/fcimb.2024.1370062
ER  - 

@article{
author = "Filipić, Brankica and Ušjak, Dušan and Rambaher, Martina Hrast and Oljacic, Slavica and Milenković, Marina",
year = "2024",
abstract = "Antimicrobial resistance is a global threat, leading to an alarming increase in the prevalence of bacterial infections that can no longer be treated with available antibiotics. The World Health Organization estimates that by 2050 up to 10 million deaths per year could be associated with antimicrobial resistance, which would equal the annual number of cancer deaths worldwide. To overcome this emerging crisis, novel anti-bacterial compounds are urgently needed. There are two possible approaches in the fight against bacterial infections: a) targeting structures within bacterial cells, similar to existing antibiotics; and/or b) targeting virulence factors rather than bacterial growth. Here, for the first time, we provide a comprehensive overview of the key steps in the evaluation of potential new anti-bacterial and/or anti-virulence compounds. The methods described in this review include: a) in silico methods for the evaluation of novel compounds; b) anti-bacterial assays (MIC, MBC, Time-kill); b) anti-virulence assays (anti-biofilm, anti-quorum sensing, anti-adhesion); and c) evaluation of safety aspects (cytotoxicity assay and Ames test). Overall, we provide a detailed description of the methods that are an essential tool for chemists, computational chemists, microbiologists, and toxicologists in the evaluation of potential novel antimicrobial compounds. These methods are cost-effective and have high predictive value. They are widely used in preclinical studies to identify new molecular candidates, for further investigation in animal and human trials.",
publisher = "Frontiers",
journal = "Frontiers in Cellular and Infection Microbiology",
title = "Evaluation of novel compounds as anti-bacterial or anti-virulence agents",
volume = "14",
doi = "10.3389/fcimb.2024.1370062"
}

Filipić, B., Ušjak, D., Rambaher, M. H., Oljacic, S.,& Milenković, M.. (2024). Evaluation of novel compounds as anti-bacterial or anti-virulence agents. in Frontiers in Cellular and Infection Microbiology
Frontiers., 14.
https://doi.org/10.3389/fcimb.2024.1370062

Filipić B, Ušjak D, Rambaher MH, Oljacic S, Milenković M. Evaluation of novel compounds as anti-bacterial or anti-virulence agents. in Frontiers in Cellular and Infection Microbiology. 2024;14.
doi:10.3389/fcimb.2024.1370062 .

Filipić, Brankica, Ušjak, Dušan, Rambaher, Martina Hrast, Oljacic, Slavica, Milenković, Marina, "Evaluation of novel compounds as anti-bacterial or anti-virulence agents" in Frontiers in Cellular and Infection Microbiology, 14 (2024),
https://doi.org/10.3389/fcimb.2024.1370062 . .

TY  - CONF
AU  - Kojić, Snežana
AU  - Bošković, Srđan
AU  - Milovanović, Mina
AU  - Stainie, Didier
AU  - Juez, Rubén Marín
AU  - Jasnić, Jovana
AU  - Novković, Mirjana
AU  - Milošević, Emilija
PY  - 2024
UR  - https://www.izfs.org/education/10sczi
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2309
AB  - In contrast to humans, zebrafish have a remarkable ability to regenerate their hearts after injury, while both humans and zebrafish efficiently repair the wounded skeletal muscle. Common players in these two processes might represent potential targets for the development of efficient therapies to stimulate human heart to regenerate after injury. We identified ankrd1a expression to be upregulated in both regenerating zebrafish hearts and in repairing skeletal muscle. Its mammalian homolog ANKRD1/CARP encodes a stress responsive cardiac ankyrin repeat protein involved in transcriptional regulation, sarcomere assembly and mechanosensing. Using a TgBAC(ankrd1a:EGFP) line, we showed that activation of ankrd1a in cryoinjured heart is restricted to border zone cardiomyocytes, implicating this gene in dedifferentiation and proliferation of regenerating cardiomyocytes. After stab wound injury of skeletal muscle expression of the fluorescent reporter was observed from 3 dpi, when new EGFP-positive muscle cells emerged inside the injury zone. At later time points, EGFP-positive myofibers were visible in the deeper tissue layers, concomitant with active repair of the injured tissue. In cryoinjured skeletal muscle, strong activation of ankrd1a was also observed in myofibers adjacent to the injury, and in those on uninjured side. Detection of the transgene in both newly formed myofibers that invade the wound and in the apparently uninjured tissue surrounding the injury suggests the role of ankrd1a in skeletal muscle tissue repair and adaptive processes in uninjured myofibers surrounding the injury site. Our results implicate ankrd1a in zebrafish muscle regeneration, repair and remodeling, promoting it as an attractive target for translational studies, as a player in muscle healing and as a sensor of stressed muscle.
PB  - Society for Zebrafish Research
C3  - 10th Strategic Conference of Zebrafish Investigators
T1  - Zebrafish ankrd1a as a common player  in heart regeneration and skeletal muscle repair
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2309
ER  - 

@conference{
author = "Kojić, Snežana and Bošković, Srđan and Milovanović, Mina and Stainie, Didier and Juez, Rubén Marín and Jasnić, Jovana and Novković, Mirjana and Milošević, Emilija",
year = "2024",
abstract = "In contrast to humans, zebrafish have a remarkable ability to regenerate their hearts after injury, while both humans and zebrafish efficiently repair the wounded skeletal muscle. Common players in these two processes might represent potential targets for the development of efficient therapies to stimulate human heart to regenerate after injury. We identified ankrd1a expression to be upregulated in both regenerating zebrafish hearts and in repairing skeletal muscle. Its mammalian homolog ANKRD1/CARP encodes a stress responsive cardiac ankyrin repeat protein involved in transcriptional regulation, sarcomere assembly and mechanosensing. Using a TgBAC(ankrd1a:EGFP) line, we showed that activation of ankrd1a in cryoinjured heart is restricted to border zone cardiomyocytes, implicating this gene in dedifferentiation and proliferation of regenerating cardiomyocytes. After stab wound injury of skeletal muscle expression of the fluorescent reporter was observed from 3 dpi, when new EGFP-positive muscle cells emerged inside the injury zone. At later time points, EGFP-positive myofibers were visible in the deeper tissue layers, concomitant with active repair of the injured tissue. In cryoinjured skeletal muscle, strong activation of ankrd1a was also observed in myofibers adjacent to the injury, and in those on uninjured side. Detection of the transgene in both newly formed myofibers that invade the wound and in the apparently uninjured tissue surrounding the injury suggests the role of ankrd1a in skeletal muscle tissue repair and adaptive processes in uninjured myofibers surrounding the injury site. Our results implicate ankrd1a in zebrafish muscle regeneration, repair and remodeling, promoting it as an attractive target for translational studies, as a player in muscle healing and as a sensor of stressed muscle.",
publisher = "Society for Zebrafish Research",
journal = "10th Strategic Conference of Zebrafish Investigators",
title = "Zebrafish ankrd1a as a common player  in heart regeneration and skeletal muscle repair",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2309"
}

Kojić, S., Bošković, S., Milovanović, M., Stainie, D., Juez, R. M., Jasnić, J., Novković, M.,& Milošević, E.. (2024). Zebrafish ankrd1a as a common player  in heart regeneration and skeletal muscle repair. in 10th Strategic Conference of Zebrafish Investigators
Society for Zebrafish Research..
https://hdl.handle.net/21.15107/rcub_imagine_2309

Kojić S, Bošković S, Milovanović M, Stainie D, Juez RM, Jasnić J, Novković M, Milošević E. Zebrafish ankrd1a as a common player  in heart regeneration and skeletal muscle repair. in 10th Strategic Conference of Zebrafish Investigators. 2024;.
https://hdl.handle.net/21.15107/rcub_imagine_2309 .

Kojić, Snežana, Bošković, Srđan, Milovanović, Mina, Stainie, Didier, Juez, Rubén Marín, Jasnić, Jovana, Novković, Mirjana, Milošević, Emilija, "Zebrafish ankrd1a as a common player  in heart regeneration and skeletal muscle repair" in 10th Strategic Conference of Zebrafish Investigators (2024),
https://hdl.handle.net/21.15107/rcub_imagine_2309 .

TY  - GEN
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2308
AB  - Echinococcus, tapeworms of the Taeniidae family, can infect humans and animals and cause
serious, even lethal disease. Global population genetics data suggests that disease presentation,
severity, immune response, as well as relative host susceptibility and resistance to infection
depend on the species, genotype and haplotype. The species of significant clinical relevance in
Europe are E. granulosus, the causative agent of cystic echinococcosis (CE) and E. multilocularis,
which causes the most severe disease, alveolar echinococcosis (AE). Analysis of the population
genetics of Echinococcus is an ongoing effort in some parts of Europe, while the Balkans
represent a significant knowledge gap. This project aims to comprehensively survey the entire
transmission cycle consisting of intermediate and definitive animal hosts and the environment
using sample processing and analytical methods which have been standardized, validated and
harmonized at the EU level to obtain high quality population genetics data via mitochondrial gene
(cox1 and nad1) sequencing and characterization of the EmsB microsatellite from single eggs,
worms and protoscolices. As a main novelty, comprehensive Echinococcus population genetics
data, through a survey of underexplored reservoirs with a high transmission capacity to humans,
will be systematized and graphically displayed through an interactive bioinformatics database,
WormProfiler, with a user interface tailored to physicians and veterinarians. The impact of the
project is the translation of population genetics data to physicians and veterinarians, key
stakeholders for transmission prevention to facilitate education of the public and raise awareness
of echinococcosis. The project should provide the insight into the genetic diversity of
Echinococcus and identification of transmission foci, as well as a software supported framework
for systematic surveillance and future development of targeted transmission control actions to
reduce echinococcosis case burden.
T2  - Science Fund of the Republic of Serbia, Program PROMIS
T1  - Worm Profiler: Surveillance and population genetics of Echinococcus in Serbia (WORM_PROFILER)
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2308
ER  - 

@misc{
year = "2024",
abstract = "Echinococcus, tapeworms of the Taeniidae family, can infect humans and animals and cause
serious, even lethal disease. Global population genetics data suggests that disease presentation,
severity, immune response, as well as relative host susceptibility and resistance to infection
depend on the species, genotype and haplotype. The species of significant clinical relevance in
Europe are E. granulosus, the causative agent of cystic echinococcosis (CE) and E. multilocularis,
which causes the most severe disease, alveolar echinococcosis (AE). Analysis of the population
genetics of Echinococcus is an ongoing effort in some parts of Europe, while the Balkans
represent a significant knowledge gap. This project aims to comprehensively survey the entire
transmission cycle consisting of intermediate and definitive animal hosts and the environment
using sample processing and analytical methods which have been standardized, validated and
harmonized at the EU level to obtain high quality population genetics data via mitochondrial gene
(cox1 and nad1) sequencing and characterization of the EmsB microsatellite from single eggs,
worms and protoscolices. As a main novelty, comprehensive Echinococcus population genetics
data, through a survey of underexplored reservoirs with a high transmission capacity to humans,
will be systematized and graphically displayed through an interactive bioinformatics database,
WormProfiler, with a user interface tailored to physicians and veterinarians. The impact of the
project is the translation of population genetics data to physicians and veterinarians, key
stakeholders for transmission prevention to facilitate education of the public and raise awareness
of echinococcosis. The project should provide the insight into the genetic diversity of
Echinococcus and identification of transmission foci, as well as a software supported framework
for systematic surveillance and future development of targeted transmission control actions to
reduce echinococcosis case burden.",
journal = "Science Fund of the Republic of Serbia, Program PROMIS",
title = "Worm Profiler: Surveillance and population genetics of Echinococcus in Serbia (WORM_PROFILER)",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2308"
}

(2024). Worm Profiler: Surveillance and population genetics of Echinococcus in Serbia (WORM_PROFILER). in Science Fund of the Republic of Serbia, Program PROMIS.
https://hdl.handle.net/21.15107/rcub_imagine_2308

Worm Profiler: Surveillance and population genetics of Echinococcus in Serbia (WORM_PROFILER). in Science Fund of the Republic of Serbia, Program PROMIS. 2024;.
https://hdl.handle.net/21.15107/rcub_imagine_2308 .

"Worm Profiler: Surveillance and population genetics of Echinococcus in Serbia (WORM_PROFILER)" in Science Fund of the Republic of Serbia, Program PROMIS (2024),
https://hdl.handle.net/21.15107/rcub_imagine_2308 .

TY  - JOUR
AU  - Spasovski, Vesna
AU  - Romolo, Anna
AU  - Zagorc, Urška
AU  - Arrigler, Vesna
AU  - Kisovec, Matic
AU  - Zavec, Apolonija Bedina
AU  - Arko, Matevž
AU  - Molnár, Adrienn
AU  - Schlosser, Gitta
AU  - Iglič, Aleš
AU  - Kogej, Ksenija
AU  - Kralj-Iglič, Veronika
PY  - 2024
UR  - https://www.dovepress.com/characterization-of-nanohybridosomes-from-lipids-and-spruce-homogenate-peer-reviewed-fulltext-article-IJN
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2332
AB  - Introduction: Lipid nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called nano-sized
hybridosomes or nanohybridosomes, NSHs) were considered.
Methods: We formed NSHs by mixing appropriate amounts of lecithin, glycerol and supernatant of isolation of extracellular vesicles
from spruce needle homogenate. We visualized NSHs by light microscopy and cryogenic transmission electron microscopy and
assessed them by flow cytometry, dynamic light scattering, ultraviolet–visual spectroscopy, interferometric light microscopy and liquid
chromatography–mass spectrometry.
Results: We found that the particles consisted of a bilayer membrane and a fluid-like interior. Flow cytometry and
interferometric light microscopy measurements showed that the majority of the particles were nano-sized. Dynamic light
scattering and interferometric light microscopy measurements agreed well on the average hydrodynamic radius of the
particles Rh (between 140 and 180 nm), while the concentrations of the particles were in the range between 1013 and
1014/mL indicating that NSHs present a considerable (more than 25%) of the sample which is much more than the yield of
natural extracellular vesicles (EVs) from spruce needle homogenate (estimated less than 1%). Spruce specific lipids and
proteins were found in hybridosomes.
Discussion: Simple and low-cost preparation method, non-demanding saving process and efficient formation procedure suggest that
large-scale production of NSHs from lipids and spruce needle homogenate is feasible.
Plain Language Summary: Cells shed into their exterior nanoparticles (here referred to as extracellular vesicles – EVs) that
are free to move, reach distant cells and are taken up by them. As they carry bioactive constituents, EVs may have important
impact on the recipient cells. The mechanisms of EV formation and mediation can be employed in designing therapeutic,
prophylactic and diagnostic methods for various medical issues. EVs can be harvested from biological samples; however,
their yield is small,12 and there are potential side effects. Artificial vesicles – liposomes – have high yield; however, in vivo,
they can be degraded before reaching the target and their reproducibility is yet insufficient. In order to combine advantages of
both types of nanoparticles, we have composed nanohybridosomes (NSHs) from soya lecithin, water and supernatant of
isolation of EVs from spruce needle homogenate, visualized them by cryogenic electron microscopy and characterized them
with respect to their size, concentration and protein/nucleic acid content. We have applied a recently developed interferometric light microscopy to determine the hydrodynamic radius and the concentration of EVs. We found that the majority of
composed particles are nano-sized and that they enclose more than 25% of the incoming volume of liquid, which is considerably more than about 1% that can be harvested by isolation of EVs from spruce needle homogenate by (ultra)
centrifugation
PB  - Dove Press Ltd
T2  - International Journal of Nanomedicine
T1  - Characterization of Nanohybridosomes from Lipids and Spruce Homogenate Containing Extracellular Vesicles
EP  - 1721
SP  - 1709
VL  - 19
DO  - 10.2147/IJN.S432836
ER  - 

@article{
author = "Spasovski, Vesna and Romolo, Anna and Zagorc, Urška and Arrigler, Vesna and Kisovec, Matic and Zavec, Apolonija Bedina and Arko, Matevž and Molnár, Adrienn and Schlosser, Gitta and Iglič, Aleš and Kogej, Ksenija and Kralj-Iglič, Veronika",
year = "2024",
abstract = "Introduction: Lipid nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called nano-sized
hybridosomes or nanohybridosomes, NSHs) were considered.
Methods: We formed NSHs by mixing appropriate amounts of lecithin, glycerol and supernatant of isolation of extracellular vesicles
from spruce needle homogenate. We visualized NSHs by light microscopy and cryogenic transmission electron microscopy and
assessed them by flow cytometry, dynamic light scattering, ultraviolet–visual spectroscopy, interferometric light microscopy and liquid
chromatography–mass spectrometry.
Results: We found that the particles consisted of a bilayer membrane and a fluid-like interior. Flow cytometry and
interferometric light microscopy measurements showed that the majority of the particles were nano-sized. Dynamic light
scattering and interferometric light microscopy measurements agreed well on the average hydrodynamic radius of the
particles Rh (between 140 and 180 nm), while the concentrations of the particles were in the range between 1013 and
1014/mL indicating that NSHs present a considerable (more than 25%) of the sample which is much more than the yield of
natural extracellular vesicles (EVs) from spruce needle homogenate (estimated less than 1%). Spruce specific lipids and
proteins were found in hybridosomes.
Discussion: Simple and low-cost preparation method, non-demanding saving process and efficient formation procedure suggest that
large-scale production of NSHs from lipids and spruce needle homogenate is feasible.
Plain Language Summary: Cells shed into their exterior nanoparticles (here referred to as extracellular vesicles – EVs) that
are free to move, reach distant cells and are taken up by them. As they carry bioactive constituents, EVs may have important
impact on the recipient cells. The mechanisms of EV formation and mediation can be employed in designing therapeutic,
prophylactic and diagnostic methods for various medical issues. EVs can be harvested from biological samples; however,
their yield is small,12 and there are potential side effects. Artificial vesicles – liposomes – have high yield; however, in vivo,
they can be degraded before reaching the target and their reproducibility is yet insufficient. In order to combine advantages of
both types of nanoparticles, we have composed nanohybridosomes (NSHs) from soya lecithin, water and supernatant of
isolation of EVs from spruce needle homogenate, visualized them by cryogenic electron microscopy and characterized them
with respect to their size, concentration and protein/nucleic acid content. We have applied a recently developed interferometric light microscopy to determine the hydrodynamic radius and the concentration of EVs. We found that the majority of
composed particles are nano-sized and that they enclose more than 25% of the incoming volume of liquid, which is considerably more than about 1% that can be harvested by isolation of EVs from spruce needle homogenate by (ultra)
centrifugation",
publisher = "Dove Press Ltd",
journal = "International Journal of Nanomedicine",
title = "Characterization of Nanohybridosomes from Lipids and Spruce Homogenate Containing Extracellular Vesicles",
pages = "1721-1709",
volume = "19",
doi = "10.2147/IJN.S432836"
}

Spasovski, V., Romolo, A., Zagorc, U., Arrigler, V., Kisovec, M., Zavec, A. B., Arko, M., Molnár, A., Schlosser, G., Iglič, A., Kogej, K.,& Kralj-Iglič, V.. (2024). Characterization of Nanohybridosomes from Lipids and Spruce Homogenate Containing Extracellular Vesicles. in International Journal of Nanomedicine
Dove Press Ltd., 19, 1709-1721.
https://doi.org/10.2147/IJN.S432836

Spasovski V, Romolo A, Zagorc U, Arrigler V, Kisovec M, Zavec AB, Arko M, Molnár A, Schlosser G, Iglič A, Kogej K, Kralj-Iglič V. Characterization of Nanohybridosomes from Lipids and Spruce Homogenate Containing Extracellular Vesicles. in International Journal of Nanomedicine. 2024;19:1709-1721.
doi:10.2147/IJN.S432836 .

Spasovski, Vesna, Romolo, Anna, Zagorc, Urška, Arrigler, Vesna, Kisovec, Matic, Zavec, Apolonija Bedina, Arko, Matevž, Molnár, Adrienn, Schlosser, Gitta, Iglič, Aleš, Kogej, Ksenija, Kralj-Iglič, Veronika, "Characterization of Nanohybridosomes from Lipids and Spruce Homogenate Containing Extracellular Vesicles" in International Journal of Nanomedicine, 19 (2024):1709-1721,
https://doi.org/10.2147/IJN.S432836 . .

TY  - JOUR
AU  - Taxeidis, George
AU  - Đapović, Milica
AU  - Nikolaivits, Efstratios
AU  - Maslak, Veselin
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
PY  - 2024
UR  - https://doi.org/10.1021/acssuschemeng.4c00143
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2339
AB  - The discovery and engineering of novel biocatalysts capable of depolymerizing polyethylene terephthalate (PET) have gained significant attention since the need for green technologies to combat plastic pollution has become increasingly urgent. This study focuses on the development of novel substrates that can indicate enzymes with PET hydrolytic activity, streamlining the process of enzyme evaluation and selection. Four novel substrates, mimicking the structure of PET, were chemically synthesized and labeled with fluorogenic or chromogenic moieties, enabling the direct analysis of candidate enzymes without complex preparatory or analysis steps. The fluorogenic substrates, mUPET1, mUPET2, and mUPET3, not only identify enzymes capable of PET breakdown but also differentiate those with exceptional performance on the polymer, such as the benchmark PETase, LCCICCG. Among the substrates, the chromogenic p-NPhPET3 stands out as a reliable tool for screening both pure and crude enzymes, offering advantages over fluorogenic substrates such as ease of assay using UV–vis spectroscopy and compatibility with crude enzyme samples. However, ferulic acid esterases and mono-(2-hydroxyethyl) terephthalate esterases (MHETases), which exhibit remarkably high affinity for PET oligomers, also show high catalytic activity on these substrates. The substrates introduced in this study hold significant value in the function-based screening and characterization of enzymes that degrade PET, as well as the the potential to be used in screening mutant libraries derived from directed evolution experiments. Following this approach, a rapid and dependable assay method can be carried out using basic laboratory infrastructure, eliminating the necessity for intricate preparatory procedures before analysis.
PB  - American Chemical Society
T2  - ACS Sustainable Chemistry & Engineering
T1  - New Labeled PET Analogues Enable the Functional Screening and Characterization of PET-Degrading Enzymes
DO  - 10.1021/acssuschemeng.4c00143
ER  - 

@article{
author = "Taxeidis, George and Đapović, Milica and Nikolaivits, Efstratios and Maslak, Veselin and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
year = "2024",
abstract = "The discovery and engineering of novel biocatalysts capable of depolymerizing polyethylene terephthalate (PET) have gained significant attention since the need for green technologies to combat plastic pollution has become increasingly urgent. This study focuses on the development of novel substrates that can indicate enzymes with PET hydrolytic activity, streamlining the process of enzyme evaluation and selection. Four novel substrates, mimicking the structure of PET, were chemically synthesized and labeled with fluorogenic or chromogenic moieties, enabling the direct analysis of candidate enzymes without complex preparatory or analysis steps. The fluorogenic substrates, mUPET1, mUPET2, and mUPET3, not only identify enzymes capable of PET breakdown but also differentiate those with exceptional performance on the polymer, such as the benchmark PETase, LCCICCG. Among the substrates, the chromogenic p-NPhPET3 stands out as a reliable tool for screening both pure and crude enzymes, offering advantages over fluorogenic substrates such as ease of assay using UV–vis spectroscopy and compatibility with crude enzyme samples. However, ferulic acid esterases and mono-(2-hydroxyethyl) terephthalate esterases (MHETases), which exhibit remarkably high affinity for PET oligomers, also show high catalytic activity on these substrates. The substrates introduced in this study hold significant value in the function-based screening and characterization of enzymes that degrade PET, as well as the the potential to be used in screening mutant libraries derived from directed evolution experiments. Following this approach, a rapid and dependable assay method can be carried out using basic laboratory infrastructure, eliminating the necessity for intricate preparatory procedures before analysis.",
publisher = "American Chemical Society",
journal = "ACS Sustainable Chemistry & Engineering",
title = "New Labeled PET Analogues Enable the Functional Screening and Characterization of PET-Degrading Enzymes",
doi = "10.1021/acssuschemeng.4c00143"
}

Taxeidis, G., Đapović, M., Nikolaivits, E., Maslak, V., Nikodinović-Runić, J.,& Topakas, E.. (2024). New Labeled PET Analogues Enable the Functional Screening and Characterization of PET-Degrading Enzymes. in ACS Sustainable Chemistry & Engineering
American Chemical Society..
https://doi.org/10.1021/acssuschemeng.4c00143

Taxeidis G, Đapović M, Nikolaivits E, Maslak V, Nikodinović-Runić J, Topakas E. New Labeled PET Analogues Enable the Functional Screening and Characterization of PET-Degrading Enzymes. in ACS Sustainable Chemistry & Engineering. 2024;.
doi:10.1021/acssuschemeng.4c00143 .

Taxeidis, George, Đapović, Milica, Nikolaivits, Efstratios, Maslak, Veselin, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "New Labeled PET Analogues Enable the Functional Screening and Characterization of PET-Degrading Enzymes" in ACS Sustainable Chemistry & Engineering (2024),
https://doi.org/10.1021/acssuschemeng.4c00143 . .

TY  - CONF
AU  - Drakulić, Danijela
AU  - Petrakis, Spyros
AU  - Harwood, Adrian J.
AU  - Linden, David
AU  - Lazić, Andrijana
AU  - Kovačević-Grujičić, Nataša
AU  - Stevanović, Milena
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1325
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2361
AB  - Neurodevelopmental disorders (NDDs) are caused by alterations in early brain development. They are a group of geographically dispersed, complex and heterogeneous disorders that give rise to the psychiatric conditions such as autism spectrum disorders, intellectual disability, schizophrenia and bipolar disorder. In order to build global research activity for study of NDDs, the main goals of the Twinning project STREAMLINE are to enhanced strategic networking and reinforce research and innovation potential of the Institute of Molecular Genetics and Genetic Engineering, University of Belgrade (IMGGE) in order to develop IMGGE as a high capacity hub for research of NDDs in the Western Balkans. This will be achieved by twinning IMGGE with three top-class research institutions in Europe (Cardiff University, University of Maastricht and Centre for Research and Technology Hellas) with an exceptional expertise in the stem cells based research of NDDs, -OMICS technologies, bioinformatics data analysis and drug testing and through staff exchanges, training, and organization of summer schools, Industry Open Days, symposia and workshops.
C3  - Hemijska industrija (Chemical Industry)
T1  - STREAMLINE HUB: a high capacity hub for research of neurodevelopmental disorders in the Western Balkan region
EP  - 78
IS  - 1S
SP  - 78
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2361
ER  - 

@conference{
author = "Drakulić, Danijela and Petrakis, Spyros and Harwood, Adrian J. and Linden, David and Lazić, Andrijana and Kovačević-Grujičić, Nataša and Stevanović, Milena",
year = "2024",
abstract = "Neurodevelopmental disorders (NDDs) are caused by alterations in early brain development. They are a group of geographically dispersed, complex and heterogeneous disorders that give rise to the psychiatric conditions such as autism spectrum disorders, intellectual disability, schizophrenia and bipolar disorder. In order to build global research activity for study of NDDs, the main goals of the Twinning project STREAMLINE are to enhanced strategic networking and reinforce research and innovation potential of the Institute of Molecular Genetics and Genetic Engineering, University of Belgrade (IMGGE) in order to develop IMGGE as a high capacity hub for research of NDDs in the Western Balkans. This will be achieved by twinning IMGGE with three top-class research institutions in Europe (Cardiff University, University of Maastricht and Centre for Research and Technology Hellas) with an exceptional expertise in the stem cells based research of NDDs, -OMICS technologies, bioinformatics data analysis and drug testing and through staff exchanges, training, and organization of summer schools, Industry Open Days, symposia and workshops.",
journal = "Hemijska industrija (Chemical Industry)",
title = "STREAMLINE HUB: a high capacity hub for research of neurodevelopmental disorders in the Western Balkan region",
pages = "78-78",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2361"
}

Drakulić, D., Petrakis, S., Harwood, A. J., Linden, D., Lazić, A., Kovačević-Grujičić, N.,& Stevanović, M.. (2024). STREAMLINE HUB: a high capacity hub for research of neurodevelopmental disorders in the Western Balkan region. in Hemijska industrija (Chemical Industry), 78(1S), 78-78.
https://hdl.handle.net/21.15107/rcub_imagine_2361

Drakulić D, Petrakis S, Harwood AJ, Linden D, Lazić A, Kovačević-Grujičić N, Stevanović M. STREAMLINE HUB: a high capacity hub for research of neurodevelopmental disorders in the Western Balkan region. in Hemijska industrija (Chemical Industry). 2024;78(1S):78-78.
https://hdl.handle.net/21.15107/rcub_imagine_2361 .

Drakulić, Danijela, Petrakis, Spyros, Harwood, Adrian J., Linden, David, Lazić, Andrijana, Kovačević-Grujičić, Nataša, Stevanović, Milena, "STREAMLINE HUB: a high capacity hub for research of neurodevelopmental disorders in the Western Balkan region" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):78-78,
https://hdl.handle.net/21.15107/rcub_imagine_2361 .

TY  - JOUR
AU  - Atanasković, Marija
AU  - Morić, Ivana
AU  - Rokić, Miloš
AU  - Đokić, Anđela
AU  - Pantović, Jelena
AU  - Despotović, Dragana
AU  - Šenerović, Lidija
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S221242922301194X
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2331
AB  - SalmonellaEnteritidis is the most commonly reported pathogen for foodborne illness outbreaks in both underdeveloped and developed regions. S. Enteritidis biofilms, which form on various food contact surfaces, are resistant to conventional physical and chemical cleaning and disinfection procedures routinely used in food processing. The aim of this study was to identify novel, industrially applicable enzymes that are active against S. Enteritidis biofilms. We describe the properties and anti-biofilm activity of heterologously expressed β-glucosidase B derived from the environmental strain Microbacterium sp. BG28 (BglB-BG28) collected from gills of bream fish. The enzyme inhibited adhesion and the early stages of biofilm formation in clinical isolates of S. Enteritidis. At a concentration of 200 μg/mL, BglB-BG28 effectively reduced biofilm formation, by decreasing biofilm biomass by 50% and metabolic activity within biofilms by 80%. The enzyme reduced the formation of air-liquid biofilms on various surfaces, including plastic, glass and metal, as observed by fluorescence microscopy. BglB-BG28 inhibited biofilm formation in Escherichia coli, another important food pathogen that also forms cellulose-rich biofilms. Using o-NPG as substrate, the enzyme showed activity at temperatures up to 50 °C and in a pH range between 4 and 8, high tolerance to sodium chloride and glucose, and compatibility with nonionic detergents. Importantly, no toxicity was observed in the model system Caenorhabditis elegans even at an enzyme concentration of 1 mg/mL. These results suggest that the β-glucosidase BglB-BG28 is a promising candidate for the development of a new enzyme-based disinfection protocol that can be used in food processing facilities.
PB  - Elsevier
T2  - Food Bioscience
T2  - Food BioscienceFood Bioscience
T1  - Inhibition of Salmonella Enteritidis adhesion and biofilm formation by β-glucosidase B from Microbacterium sp. BG28
SP  - 103543
VL  - 57
DO  - 10.1016/j.fbio.2023.103543
ER  - 

@article{
author = "Atanasković, Marija and Morić, Ivana and Rokić, Miloš and Đokić, Anđela and Pantović, Jelena and Despotović, Dragana and Šenerović, Lidija",
year = "2024",
abstract = "SalmonellaEnteritidis is the most commonly reported pathogen for foodborne illness outbreaks in both underdeveloped and developed regions. S. Enteritidis biofilms, which form on various food contact surfaces, are resistant to conventional physical and chemical cleaning and disinfection procedures routinely used in food processing. The aim of this study was to identify novel, industrially applicable enzymes that are active against S. Enteritidis biofilms. We describe the properties and anti-biofilm activity of heterologously expressed β-glucosidase B derived from the environmental strain Microbacterium sp. BG28 (BglB-BG28) collected from gills of bream fish. The enzyme inhibited adhesion and the early stages of biofilm formation in clinical isolates of S. Enteritidis. At a concentration of 200 μg/mL, BglB-BG28 effectively reduced biofilm formation, by decreasing biofilm biomass by 50% and metabolic activity within biofilms by 80%. The enzyme reduced the formation of air-liquid biofilms on various surfaces, including plastic, glass and metal, as observed by fluorescence microscopy. BglB-BG28 inhibited biofilm formation in Escherichia coli, another important food pathogen that also forms cellulose-rich biofilms. Using o-NPG as substrate, the enzyme showed activity at temperatures up to 50 °C and in a pH range between 4 and 8, high tolerance to sodium chloride and glucose, and compatibility with nonionic detergents. Importantly, no toxicity was observed in the model system Caenorhabditis elegans even at an enzyme concentration of 1 mg/mL. These results suggest that the β-glucosidase BglB-BG28 is a promising candidate for the development of a new enzyme-based disinfection protocol that can be used in food processing facilities.",
publisher = "Elsevier",
journal = "Food Bioscience, Food BioscienceFood Bioscience",
title = "Inhibition of Salmonella Enteritidis adhesion and biofilm formation by β-glucosidase B from Microbacterium sp. BG28",
pages = "103543",
volume = "57",
doi = "10.1016/j.fbio.2023.103543"
}

Atanasković, M., Morić, I., Rokić, M., Đokić, A., Pantović, J., Despotović, D.,& Šenerović, L.. (2024). Inhibition of Salmonella Enteritidis adhesion and biofilm formation by β-glucosidase B from Microbacterium sp. BG28. in Food Bioscience
Elsevier., 57, 103543.
https://doi.org/10.1016/j.fbio.2023.103543

Atanasković M, Morić I, Rokić M, Đokić A, Pantović J, Despotović D, Šenerović L. Inhibition of Salmonella Enteritidis adhesion and biofilm formation by β-glucosidase B from Microbacterium sp. BG28. in Food Bioscience. 2024;57:103543.
doi:10.1016/j.fbio.2023.103543 .

Atanasković, Marija, Morić, Ivana, Rokić, Miloš, Đokić, Anđela, Pantović, Jelena, Despotović, Dragana, Šenerović, Lidija, "Inhibition of Salmonella Enteritidis adhesion and biofilm formation by β-glucosidase B from Microbacterium sp. BG28" in Food Bioscience, 57 (2024):103543,
https://doi.org/10.1016/j.fbio.2023.103543 . .

TY  - GEN
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2316
AB  - During 2024, IMGGE researchers will continue exploring following areas:
- human molecular genetics and genomics,
- microbiology and ecology of microorganisms,
- plant molecular biology.
IMGGE researchers will focus on studying molecular mechanisms responsible for the
occurrence of selected rare and non-contagious diseases, as well as on identification of
molecular markers important for diagnosis, prognosis, therapy and prevention. Disease
modeling methodology will be developed using in vitro model systems (2D and 3D primary and
permanent cell lines, induced pluripotent stem cells) and in vivo model systems that include
different animal models. These stydies will aim at discovering the causes of the disease,
identifying new therapeutic targets, testing new therapeutics as well as individual response to
existing therapeutics.
During 2024, the existing IMGGE collection of microorganisms will be further expanded with
new isolates (industrial microorganisms, clinically relevant pathogens, and bacteriophages), and
IMGGI collaborators will devote themselves to studying the antimicrobial and antiviral potential
and application of new isolates, as well as mechanisms of resistance and virulence. The
genomes of selected microorganisms will be sequenced and analyzed to identify new genes of
interest, e.g. producers of bioactive proteins, enzymes and biosynthetic pathways.
Metagenomes of selected environmental or clinical samples will also be sequenced and
analyzed in order to study the biological diversity of complex communities of microorganisms.
When it comes to the molecular biology of plants, the response mechanisms of different model
organisms to abiotic and biotic stress will further be investigated. IMGGE researchers will
analyze the role of different proteins in maintaining cell homeostasis (eg LEA) and genome
stability (Ustilago maydis). The genomes, epigenomes and proteomes of the muscat flower will
be sequenced in order to study the response to stress.
T2  - Ministry of Science, Technological Development and Innovation of the Republic of Serbia, Annual Research Program
T1  - IMGGE Annual Research Program 2024
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2316
ER  - 

@misc{
year = "2024",
abstract = "During 2024, IMGGE researchers will continue exploring following areas:
- human molecular genetics and genomics,
- microbiology and ecology of microorganisms,
- plant molecular biology.
IMGGE researchers will focus on studying molecular mechanisms responsible for the
occurrence of selected rare and non-contagious diseases, as well as on identification of
molecular markers important for diagnosis, prognosis, therapy and prevention. Disease
modeling methodology will be developed using in vitro model systems (2D and 3D primary and
permanent cell lines, induced pluripotent stem cells) and in vivo model systems that include
different animal models. These stydies will aim at discovering the causes of the disease,
identifying new therapeutic targets, testing new therapeutics as well as individual response to
existing therapeutics.
During 2024, the existing IMGGE collection of microorganisms will be further expanded with
new isolates (industrial microorganisms, clinically relevant pathogens, and bacteriophages), and
IMGGI collaborators will devote themselves to studying the antimicrobial and antiviral potential
and application of new isolates, as well as mechanisms of resistance and virulence. The
genomes of selected microorganisms will be sequenced and analyzed to identify new genes of
interest, e.g. producers of bioactive proteins, enzymes and biosynthetic pathways.
Metagenomes of selected environmental or clinical samples will also be sequenced and
analyzed in order to study the biological diversity of complex communities of microorganisms.
When it comes to the molecular biology of plants, the response mechanisms of different model
organisms to abiotic and biotic stress will further be investigated. IMGGE researchers will
analyze the role of different proteins in maintaining cell homeostasis (eg LEA) and genome
stability (Ustilago maydis). The genomes, epigenomes and proteomes of the muscat flower will
be sequenced in order to study the response to stress.",
journal = "Ministry of Science, Technological Development and Innovation of the Republic of Serbia, Annual Research Program",
title = "IMGGE Annual Research Program 2024",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2316"
}

(2024). IMGGE Annual Research Program 2024. in Ministry of Science, Technological Development and Innovation of the Republic of Serbia, Annual Research Program.
https://hdl.handle.net/21.15107/rcub_imagine_2316

IMGGE Annual Research Program 2024. in Ministry of Science, Technological Development and Innovation of the Republic of Serbia, Annual Research Program. 2024;.
https://hdl.handle.net/21.15107/rcub_imagine_2316 .

"IMGGE Annual Research Program 2024" in Ministry of Science, Technological Development and Innovation of the Republic of Serbia, Annual Research Program (2024),
https://hdl.handle.net/21.15107/rcub_imagine_2316 .

TY  - JOUR
AU  - Virijević, Katarina
AU  - Živanović, Marko N.
AU  - Nikolić, Dalibor
AU  - Milivojević, Nevena
AU  - Pavić, Jelena
AU  - Morić, Ivana
AU  - Šenerović, Lidija
AU  - Dragačević, Luka
AU  - Thurner, Philipp J.
AU  - Rufin, Manuel
AU  - Andriotis, Orestis G.
AU  - Ljujić, Biljana
AU  - Miletić Kovačević, Marina
AU  - Papić, Miloš
AU  - Filipović, Nenad
PY  - 2024
UR  - https://doi.org/10.1021/acsami.4c03266
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2360
AB  - Here, an artificial intelligence (AI)-based approach was employed to optimize the production of electrospun scaffolds for in vivo wound healing applications. By combining polycaprolactone (PCL) and poly(ethylene glycol) (PEG) in various concentration ratios, dissolved in chloroform (CHCl3) and dimethylformamide (DMF), 125 different polymer combinations were created. From these polymer combinations, electrospun nanofiber meshes were produced and characterized structurally and mechanically via microscopic techniques, including chemical composition and fiber diameter determination. Subsequently, these data were used to train a neural network, creating an AI model to predict the optimal scaffold production solution. Guided by the predictions and experimental outcomes of the AI model, the most promising scaffold for further in vitro analyses was identified. Moreover, we enriched this selected polymer combination by incorporating antibiotics, aiming to develop electrospun nanofiber scaffolds tailored for in vivo wound healing applications. Our study underscores three noteworthy conclusions: (i) the application of AI is pivotal in the fields of material and biomedical sciences, (ii) our methodology provides an effective blueprint for the initial screening of biomedical materials, and (iii) electrospun PCL/PEG antibiotic-bearing scaffolds exhibit outstanding results in promoting neoangiogenesis and facilitating in vivo wound treatment.
PB  - American Chemical Society
T2  - ACS Applied Materials & Interfaces
T1  - AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing
DO  - 10.1021/acsami.4c03266
ER  - 

@article{
author = "Virijević, Katarina and Živanović, Marko N. and Nikolić, Dalibor and Milivojević, Nevena and Pavić, Jelena and Morić, Ivana and Šenerović, Lidija and Dragačević, Luka and Thurner, Philipp J. and Rufin, Manuel and Andriotis, Orestis G. and Ljujić, Biljana and Miletić Kovačević, Marina and Papić, Miloš and Filipović, Nenad",
year = "2024",
abstract = "Here, an artificial intelligence (AI)-based approach was employed to optimize the production of electrospun scaffolds for in vivo wound healing applications. By combining polycaprolactone (PCL) and poly(ethylene glycol) (PEG) in various concentration ratios, dissolved in chloroform (CHCl3) and dimethylformamide (DMF), 125 different polymer combinations were created. From these polymer combinations, electrospun nanofiber meshes were produced and characterized structurally and mechanically via microscopic techniques, including chemical composition and fiber diameter determination. Subsequently, these data were used to train a neural network, creating an AI model to predict the optimal scaffold production solution. Guided by the predictions and experimental outcomes of the AI model, the most promising scaffold for further in vitro analyses was identified. Moreover, we enriched this selected polymer combination by incorporating antibiotics, aiming to develop electrospun nanofiber scaffolds tailored for in vivo wound healing applications. Our study underscores three noteworthy conclusions: (i) the application of AI is pivotal in the fields of material and biomedical sciences, (ii) our methodology provides an effective blueprint for the initial screening of biomedical materials, and (iii) electrospun PCL/PEG antibiotic-bearing scaffolds exhibit outstanding results in promoting neoangiogenesis and facilitating in vivo wound treatment.",
publisher = "American Chemical Society",
journal = "ACS Applied Materials & Interfaces",
title = "AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing",
doi = "10.1021/acsami.4c03266"
}

Virijević, K., Živanović, M. N., Nikolić, D., Milivojević, N., Pavić, J., Morić, I., Šenerović, L., Dragačević, L., Thurner, P. J., Rufin, M., Andriotis, O. G., Ljujić, B., Miletić Kovačević, M., Papić, M.,& Filipović, N.. (2024). AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing. in ACS Applied Materials & Interfaces
American Chemical Society..
https://doi.org/10.1021/acsami.4c03266

Virijević K, Živanović MN, Nikolić D, Milivojević N, Pavić J, Morić I, Šenerović L, Dragačević L, Thurner PJ, Rufin M, Andriotis OG, Ljujić B, Miletić Kovačević M, Papić M, Filipović N. AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing. in ACS Applied Materials & Interfaces. 2024;.
doi:10.1021/acsami.4c03266 .

Virijević, Katarina, Živanović, Marko N., Nikolić, Dalibor, Milivojević, Nevena, Pavić, Jelena, Morić, Ivana, Šenerović, Lidija, Dragačević, Luka, Thurner, Philipp J., Rufin, Manuel, Andriotis, Orestis G., Ljujić, Biljana, Miletić Kovačević, Marina, Papić, Miloš, Filipović, Nenad, "AI-Driven Optimization of PCL/PEG Electrospun Scaffolds for Enhanced In Vivo Wound Healing" in ACS Applied Materials & Interfaces (2024),
https://doi.org/10.1021/acsami.4c03266 . .

TY  - JOUR
AU  - Novković, Mirjana
AU  - Banović Đeri, Bojana
AU  - RistivojeviĆ, Bojan
AU  - Knežević, Aleksandra
AU  - Janković, Marko
AU  - Tanasić, Vanja
AU  - Radojičić, Verica
AU  - Keckarević, Dusan
AU  - Vidanović, Dejan
AU  - Tešović, Bojana
AU  - Skakić, Anita
AU  - Tolinački, Maja
AU  - Morić, Ivana
AU  - Đorđević, Valentina
PY  - 2024
UR  - https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1332276
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2327
AB  - The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), responsible for the COVID-19 pandemic, has been evolving rapidly causing emergence of new variants and health uncertainties. Monitoring the evolution of the virus was of the utmost importance for public health interventions and the development of national and global mitigation strategies. Here, we report national data on the emergence of new variants, their distribution, and dynamics in a 3-year study conducted from March 2020 to the end of January 2023 in the Republic of Serbia. Nasopharyngeal and oropharyngeal swabs from 2,398 COVID-19-positive patients were collected and sequenced using three different next generation technologies: Oxford Nanopore, Ion Torrent, and DNBSeq. In the subset of 2,107 SARS-CoV-2 sequences which met the quality requirements, detection of mutations, assignment to SARS-CoV-2 lineages, and phylogenetic analysis were performed. During the 3-year period, we detected three variants of concern, namely, Alpha (5.6%), Delta (7.4%), and Omicron (70.3%) and one variant of interest—Omicron recombinant “Kraken” (XBB1.5) (<1%), whereas 16.8% of the samples belonged to other SARS-CoV-2 (sub)lineages. The detected SARS-CoV-2 (sub)lineages resulted in eight COVID-19 pandemic waves in Serbia, which correspond to the pandemic waves reported in Europe and the United States. Wave dynamics in Serbia showed the most resemblance with the profile of pandemic waves in southern Europe, consistent with the southeastern European location of Serbia. The samples were assigned to sixteen SARS-CoV-2 Nextstrain clades: 20A, 20B, 20C, 20D, 20E, 20G, 20I, 21J, 21K, 21L, 22A, 22B, 22C, 22D, 22E, and 22F and six different Omicron recombinants (XZ, XAZ, XAS, XBB, XBF, and XBK). The 10 most common mutations detected in the coding and untranslated regions of the SARS-CoV-2 genomes included four mutations affecting the spike protein (S:D614G, S:T478K, S:P681H, and S:S477N) and one mutation at each of the following positions: 5′-untranslated region (5’UTR:241); N protein (N:RG203KR); NSP3 protein (NSP3:F106F); NSP4 protein (NSP4:T492I); NSP6 protein (NSP6: S106/G107/F108 - triple deletion), and NSP12b protein (NSP12b:P314L). This national-level study is the most comprehensive in terms of sequencing and genomic surveillance of SARS-CoV-2 during the pandemic in Serbia, highlighting the importance of establishing and maintaining good national practice for monitoring SARS-CoV-2 and other viruses circulating worldwide.
AB  - The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), responsible for the COVID-19 pandemic, has been evolving rapidly causing emergence of new variants and health uncertainties. Monitoring the evolution of the virus was of the utmost importance for public health interventions and the development of national and global mitigation strategies. Here, we report national data on the emergence of new variants, their distribution, and dynamics in a 3-year study conducted from March 2020 to the end of January 2023 in the Republic of Serbia. Nasopharyngeal and oropharyngeal swabs from 2,398 COVID-19- positive patients were collected and sequenced using three different next generation technologies: Oxford Nanopore, Ion Torrent, and DNBSeq. In the subset of 2,107 SARS-CoV-2 sequences which met the quality requirements, detection of mutations, assignment to SARS-CoV-2 lineages, and phylogenetic analysis were performed. During the 3-year period, we detected three variants of concern, namely, Alpha (5.6%), Delta (7.4%), and Omicron (70.3%) and one variant of interest—Omicron recombinant “Kraken” (XBB1.5) (<1%), whereas 16.8% of the samples belonged to other SARS-CoV-2 (sub)lineages. The detected SARS-CoV-2 (sub)lineages resulted in eight COVID-19 pandemic waves in Serbia, which correspond to the pandemic waves reported in Europe and the United States. Wave dynamics in Serbia showed the most resemblance with the profile of pandemic waves in southern Europe, consistent with the southeastern European location of Serbia. The samples were assigned to sixteen SARS-CoV-2 Nextstrain clades: 20A, 20B, 20C, 20D, 20E, 20G, 20I, 21J, 21K, 21L, 22A, 22B, 22C, 22D, 22E, and 22F and six different Omicron recombinants (XZ, XAZ, XAS, XBB, XBF, and XBK). The 10 most common mutations detected in the coding and untranslated regions of the SARS-CoV-2 genomes included four mutations affecting the spike protein (S:D614G, S:T478K, S:P681H, and S:S477N) and one mutation at each of the following positions: 5′-untranslated region (5’UTR:241); N protein (N:RG203KR); NSP3 protein (NSP3:F106F); NSP4 protein (NSP4:T492I); NSP6 protein (NSP6: S106/G107/F108 - triple deletion), and NSP12b protein (NSP12b:P314L). This national-level study is the most comprehensive in terms of sequencing and genomic surveillance of SARS-CoV-2 during the pandemic in Serbia, highlighting the importance of establishing and maintaining good national practice for monitoring SARS-CoV-2 and other viruses circulating worldwide.
PB  - Frontiers
T2  - Frontiers in Microbiology
T2  - Frontiers in Microbiology
T1  - Genome sequence diversity of SARS-CoV-2 in Serbia: insights gained from a 3-year pandemic study
VL  - 15
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2327
ER  - 

@article{
author = "Novković, Mirjana and Banović Đeri, Bojana and RistivojeviĆ, Bojan and Knežević, Aleksandra and Janković, Marko and Tanasić, Vanja and Radojičić, Verica and Keckarević, Dusan and Vidanović, Dejan and Tešović, Bojana and Skakić, Anita and Tolinački, Maja and Morić, Ivana and Đorđević, Valentina",
year = "2024",
abstract = "The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), responsible for the COVID-19 pandemic, has been evolving rapidly causing emergence of new variants and health uncertainties. Monitoring the evolution of the virus was of the utmost importance for public health interventions and the development of national and global mitigation strategies. Here, we report national data on the emergence of new variants, their distribution, and dynamics in a 3-year study conducted from March 2020 to the end of January 2023 in the Republic of Serbia. Nasopharyngeal and oropharyngeal swabs from 2,398 COVID-19-positive patients were collected and sequenced using three different next generation technologies: Oxford Nanopore, Ion Torrent, and DNBSeq. In the subset of 2,107 SARS-CoV-2 sequences which met the quality requirements, detection of mutations, assignment to SARS-CoV-2 lineages, and phylogenetic analysis were performed. During the 3-year period, we detected three variants of concern, namely, Alpha (5.6%), Delta (7.4%), and Omicron (70.3%) and one variant of interest—Omicron recombinant “Kraken” (XBB1.5) (<1%), whereas 16.8% of the samples belonged to other SARS-CoV-2 (sub)lineages. The detected SARS-CoV-2 (sub)lineages resulted in eight COVID-19 pandemic waves in Serbia, which correspond to the pandemic waves reported in Europe and the United States. Wave dynamics in Serbia showed the most resemblance with the profile of pandemic waves in southern Europe, consistent with the southeastern European location of Serbia. The samples were assigned to sixteen SARS-CoV-2 Nextstrain clades: 20A, 20B, 20C, 20D, 20E, 20G, 20I, 21J, 21K, 21L, 22A, 22B, 22C, 22D, 22E, and 22F and six different Omicron recombinants (XZ, XAZ, XAS, XBB, XBF, and XBK). The 10 most common mutations detected in the coding and untranslated regions of the SARS-CoV-2 genomes included four mutations affecting the spike protein (S:D614G, S:T478K, S:P681H, and S:S477N) and one mutation at each of the following positions: 5′-untranslated region (5’UTR:241); N protein (N:RG203KR); NSP3 protein (NSP3:F106F); NSP4 protein (NSP4:T492I); NSP6 protein (NSP6: S106/G107/F108 - triple deletion), and NSP12b protein (NSP12b:P314L). This national-level study is the most comprehensive in terms of sequencing and genomic surveillance of SARS-CoV-2 during the pandemic in Serbia, highlighting the importance of establishing and maintaining good national practice for monitoring SARS-CoV-2 and other viruses circulating worldwide., The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), responsible for the COVID-19 pandemic, has been evolving rapidly causing emergence of new variants and health uncertainties. Monitoring the evolution of the virus was of the utmost importance for public health interventions and the development of national and global mitigation strategies. Here, we report national data on the emergence of new variants, their distribution, and dynamics in a 3-year study conducted from March 2020 to the end of January 2023 in the Republic of Serbia. Nasopharyngeal and oropharyngeal swabs from 2,398 COVID-19- positive patients were collected and sequenced using three different next generation technologies: Oxford Nanopore, Ion Torrent, and DNBSeq. In the subset of 2,107 SARS-CoV-2 sequences which met the quality requirements, detection of mutations, assignment to SARS-CoV-2 lineages, and phylogenetic analysis were performed. During the 3-year period, we detected three variants of concern, namely, Alpha (5.6%), Delta (7.4%), and Omicron (70.3%) and one variant of interest—Omicron recombinant “Kraken” (XBB1.5) (<1%), whereas 16.8% of the samples belonged to other SARS-CoV-2 (sub)lineages. The detected SARS-CoV-2 (sub)lineages resulted in eight COVID-19 pandemic waves in Serbia, which correspond to the pandemic waves reported in Europe and the United States. Wave dynamics in Serbia showed the most resemblance with the profile of pandemic waves in southern Europe, consistent with the southeastern European location of Serbia. The samples were assigned to sixteen SARS-CoV-2 Nextstrain clades: 20A, 20B, 20C, 20D, 20E, 20G, 20I, 21J, 21K, 21L, 22A, 22B, 22C, 22D, 22E, and 22F and six different Omicron recombinants (XZ, XAZ, XAS, XBB, XBF, and XBK). The 10 most common mutations detected in the coding and untranslated regions of the SARS-CoV-2 genomes included four mutations affecting the spike protein (S:D614G, S:T478K, S:P681H, and S:S477N) and one mutation at each of the following positions: 5′-untranslated region (5’UTR:241); N protein (N:RG203KR); NSP3 protein (NSP3:F106F); NSP4 protein (NSP4:T492I); NSP6 protein (NSP6: S106/G107/F108 - triple deletion), and NSP12b protein (NSP12b:P314L). This national-level study is the most comprehensive in terms of sequencing and genomic surveillance of SARS-CoV-2 during the pandemic in Serbia, highlighting the importance of establishing and maintaining good national practice for monitoring SARS-CoV-2 and other viruses circulating worldwide.",
publisher = "Frontiers",
journal = "Frontiers in Microbiology, Frontiers in Microbiology",
title = "Genome sequence diversity of SARS-CoV-2 in Serbia: insights gained from a 3-year pandemic study",
volume = "15",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2327"
}

Novković, M., Banović Đeri, B., RistivojeviĆ, B., Knežević, A., Janković, M., Tanasić, V., Radojičić, V., Keckarević, D., Vidanović, D., Tešović, B., Skakić, A., Tolinački, M., Morić, I.,& Đorđević, V.. (2024). Genome sequence diversity of SARS-CoV-2 in Serbia: insights gained from a 3-year pandemic study. in Frontiers in Microbiology
Frontiers., 15.
https://hdl.handle.net/21.15107/rcub_imagine_2327

Novković M, Banović Đeri B, RistivojeviĆ B, Knežević A, Janković M, Tanasić V, Radojičić V, Keckarević D, Vidanović D, Tešović B, Skakić A, Tolinački M, Morić I, Đorđević V. Genome sequence diversity of SARS-CoV-2 in Serbia: insights gained from a 3-year pandemic study. in Frontiers in Microbiology. 2024;15.
https://hdl.handle.net/21.15107/rcub_imagine_2327 .

Novković, Mirjana, Banović Đeri, Bojana, RistivojeviĆ, Bojan, Knežević, Aleksandra, Janković, Marko, Tanasić, Vanja, Radojičić, Verica, Keckarević, Dusan, Vidanović, Dejan, Tešović, Bojana, Skakić, Anita, Tolinački, Maja, Morić, Ivana, Đorđević, Valentina, "Genome sequence diversity of SARS-CoV-2 in Serbia: insights gained from a 3-year pandemic study" in Frontiers in Microbiology, 15 (2024),
https://hdl.handle.net/21.15107/rcub_imagine_2327 .

TY  - CONF
AU  - Brkušanin, Miloš
AU  - Garai, Nemanja
AU  - Karanović, Jelena
AU  - Tričković, Matija
AU  - Nikolić, Dimitrije
AU  - Šljivančanin Jakovljević, Tamara
AU  - Dimitrijević, Aleksandra
AU  - Busarać, Ana
AU  - Jovanović, Kristina
AU  - Savić-Pavicević, Dušanka
PY  - 2024
UR  - https://ghent2024.sma-europe.eu/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2338
AB  - Spinal muscular atrophy (SMA) is the prevalent genetic cause of childhood mortality.  Pioneering treatments yield utmost advantages only within the presymptomatic phase,  underlining the medical and ethical significance of newborn screening. 
In 2022, the Centre for Human Molecular Genetics initiated a pilot study of the newborn  screening for SMA, working closely alongside the University Children’s Hospital Tirsova  and Association SMA Serbia. The aim was to lay the foundation for the implementation  of statewide newborn screening for SMA in Serbia by conducting screening for ~8000  infants from the Obstetrics and Gynaecology Clinic Narodni Front over the course of a  year. In the subsequent year, we expanded the initiative to include another maternity  hospital located outside Belgrade, introducing sample shipment via postal services and  extending screening accessibility to a greater number of infants. 
In the initial year, 6 950 newborns underwent testing, revealing SMA in two unrelated  infants. Subsequently, an older sibling of the first newborn, although asymptomatic at  the time, was also tested at the age of 16 months, and SMA diagnosis was confirmed  in this child as well. All three children received therapeutic interventions in <1 month  from birth. To date, they have exhibited no signs of SMA, and there have been no false 
negative outcomes among the newborns who tested negative during the screening. In
the second year, an additional 5 000 newborns underwent testing. As frontrunners in this field in Serbia, we orchestrated harmonized efforts across  various tiers of healthcare, established screening and diagnostic algorithms and  follow-up protocols. Our extensive efforts were primarily aimed at elevating awareness  among all stakeholders about the critical importance of early disease detection.  In this transformative journey, we transitioned from being isolated individuals and  visionaries who championed a singular idea to an entire community and nation that now  acknowledges the paramount significance of newborn screening. As a result, a total of  11 950 infants underwent testing during the 17-month pilot project, culminating in the  rapid incorporation of newborn screening for SMA into the national screening program,  effective as of September 14th 2023. 
Timely detection and treatment can transform SMA into a manageable condition, and  there is substantial evidence supporting its inclusion in state-wide screening programs.
PB  - SMA Europe
C3  - 4th International Congress on Spinal Muscular Atrophy
T1  - Revolutionizing Spinal Muscular Atrophy Prevention in Serbia: Implementing a  Mandatory Statewide Newborn Screening
EP  - 157
SP  - 157
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2338
ER  - 

@conference{
author = "Brkušanin, Miloš and Garai, Nemanja and Karanović, Jelena and Tričković, Matija and Nikolić, Dimitrije and Šljivančanin Jakovljević, Tamara and Dimitrijević, Aleksandra and Busarać, Ana and Jovanović, Kristina and Savić-Pavicević, Dušanka",
year = "2024",
abstract = "Spinal muscular atrophy (SMA) is the prevalent genetic cause of childhood mortality.  Pioneering treatments yield utmost advantages only within the presymptomatic phase,  underlining the medical and ethical significance of newborn screening. 
In 2022, the Centre for Human Molecular Genetics initiated a pilot study of the newborn  screening for SMA, working closely alongside the University Children’s Hospital Tirsova  and Association SMA Serbia. The aim was to lay the foundation for the implementation  of statewide newborn screening for SMA in Serbia by conducting screening for ~8000  infants from the Obstetrics and Gynaecology Clinic Narodni Front over the course of a  year. In the subsequent year, we expanded the initiative to include another maternity  hospital located outside Belgrade, introducing sample shipment via postal services and  extending screening accessibility to a greater number of infants. 
In the initial year, 6 950 newborns underwent testing, revealing SMA in two unrelated  infants. Subsequently, an older sibling of the first newborn, although asymptomatic at  the time, was also tested at the age of 16 months, and SMA diagnosis was confirmed  in this child as well. All three children received therapeutic interventions in <1 month  from birth. To date, they have exhibited no signs of SMA, and there have been no false 
negative outcomes among the newborns who tested negative during the screening. In
the second year, an additional 5 000 newborns underwent testing. As frontrunners in this field in Serbia, we orchestrated harmonized efforts across  various tiers of healthcare, established screening and diagnostic algorithms and  follow-up protocols. Our extensive efforts were primarily aimed at elevating awareness  among all stakeholders about the critical importance of early disease detection.  In this transformative journey, we transitioned from being isolated individuals and  visionaries who championed a singular idea to an entire community and nation that now  acknowledges the paramount significance of newborn screening. As a result, a total of  11 950 infants underwent testing during the 17-month pilot project, culminating in the  rapid incorporation of newborn screening for SMA into the national screening program,  effective as of September 14th 2023. 
Timely detection and treatment can transform SMA into a manageable condition, and  there is substantial evidence supporting its inclusion in state-wide screening programs.",
publisher = "SMA Europe",
journal = "4th International Congress on Spinal Muscular Atrophy",
title = "Revolutionizing Spinal Muscular Atrophy Prevention in Serbia: Implementing a  Mandatory Statewide Newborn Screening",
pages = "157-157",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2338"
}

Brkušanin, M., Garai, N., Karanović, J., Tričković, M., Nikolić, D., Šljivančanin Jakovljević, T., Dimitrijević, A., Busarać, A., Jovanović, K.,& Savić-Pavicević, D.. (2024). Revolutionizing Spinal Muscular Atrophy Prevention in Serbia: Implementing a  Mandatory Statewide Newborn Screening. in 4th International Congress on Spinal Muscular Atrophy
SMA Europe., 157-157.
https://hdl.handle.net/21.15107/rcub_imagine_2338

Brkušanin M, Garai N, Karanović J, Tričković M, Nikolić D, Šljivančanin Jakovljević T, Dimitrijević A, Busarać A, Jovanović K, Savić-Pavicević D. Revolutionizing Spinal Muscular Atrophy Prevention in Serbia: Implementing a  Mandatory Statewide Newborn Screening. in 4th International Congress on Spinal Muscular Atrophy. 2024;:157-157.
https://hdl.handle.net/21.15107/rcub_imagine_2338 .

Brkušanin, Miloš, Garai, Nemanja, Karanović, Jelena, Tričković, Matija, Nikolić, Dimitrije, Šljivančanin Jakovljević, Tamara, Dimitrijević, Aleksandra, Busarać, Ana, Jovanović, Kristina, Savić-Pavicević, Dušanka, "Revolutionizing Spinal Muscular Atrophy Prevention in Serbia: Implementing a  Mandatory Statewide Newborn Screening" in 4th International Congress on Spinal Muscular Atrophy (2024):157-157,
https://hdl.handle.net/21.15107/rcub_imagine_2338 .

TY  - GEN
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2290
AB  - Antimicrobial resistance (AMR) and air pollution have been identified as one of the most
serious threats to human health worldwide. The scarce data demonstrating their
interdependence indicates a need to obtain evidence from a broader global area, especially
from regions exposed to high air pollution. Considering that Serbia is a country struggling with
excessive antibiotics use and misuse, a high percentage of multidrug-resistant bacterial isolates,
and poor air quality, the Serbian capital Belgrade has been recognized as an interesting
research model for the effects of air pollution on the airborne transmission of AMR. After
optimization of air sampling and DNA extraction protocol, the air samples will be collected at
nine locations in the Belgrade metropolitan area selected according to air pollution level during
four seasons. The state-of-the-art shotgun metagenomic sequencing and bioinformatic analysis
of obtained sequences will provide information about microbial community composition of
airborne metagenomes. In addition, sequenced airborne metagenomes will be analyzed for the
abundance and diversity of resistomes (antibiotic and biocide/metal resistance genes) and
mobilomes using several databases and tools. Correlation analyses will offer us insight into the
effect of air pollution and seasonal variations on abundance and diversity of airborne
pathogens, resistome and mobilome in the Belgrade metropolitan area. In-depth approach of
the AirPollRes project will provide the first insights into intersection of AMR and air pollution in
the Belgrade metropolitan area, which is highly vulnerable to these health threats. As the
AirPollRes is a pioneering project in this field, the expected short-term impact is the
introduction of routine monitoring of pathogenic microbes and resistance determinants in the
air in Belgrade, while the longer-term impact will be reflected in the improvement of human
and animal health, allowing for a longer life with higher quality.
T2  - Program PROMIS
T1  - Assessment of seasonal airborne resistome dynamics in response to air pollution exposure in the Belgrade metropolitan area (AirPollRes)
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2290
ER  - 

@misc{
year = "2024",
abstract = "Antimicrobial resistance (AMR) and air pollution have been identified as one of the most
serious threats to human health worldwide. The scarce data demonstrating their
interdependence indicates a need to obtain evidence from a broader global area, especially
from regions exposed to high air pollution. Considering that Serbia is a country struggling with
excessive antibiotics use and misuse, a high percentage of multidrug-resistant bacterial isolates,
and poor air quality, the Serbian capital Belgrade has been recognized as an interesting
research model for the effects of air pollution on the airborne transmission of AMR. After
optimization of air sampling and DNA extraction protocol, the air samples will be collected at
nine locations in the Belgrade metropolitan area selected according to air pollution level during
four seasons. The state-of-the-art shotgun metagenomic sequencing and bioinformatic analysis
of obtained sequences will provide information about microbial community composition of
airborne metagenomes. In addition, sequenced airborne metagenomes will be analyzed for the
abundance and diversity of resistomes (antibiotic and biocide/metal resistance genes) and
mobilomes using several databases and tools. Correlation analyses will offer us insight into the
effect of air pollution and seasonal variations on abundance and diversity of airborne
pathogens, resistome and mobilome in the Belgrade metropolitan area. In-depth approach of
the AirPollRes project will provide the first insights into intersection of AMR and air pollution in
the Belgrade metropolitan area, which is highly vulnerable to these health threats. As the
AirPollRes is a pioneering project in this field, the expected short-term impact is the
introduction of routine monitoring of pathogenic microbes and resistance determinants in the
air in Belgrade, while the longer-term impact will be reflected in the improvement of human
and animal health, allowing for a longer life with higher quality.",
journal = "Program PROMIS",
title = "Assessment of seasonal airborne resistome dynamics in response to air pollution exposure in the Belgrade metropolitan area (AirPollRes)",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2290"
}

(2024). Assessment of seasonal airborne resistome dynamics in response to air pollution exposure in the Belgrade metropolitan area (AirPollRes). in Program PROMIS.
https://hdl.handle.net/21.15107/rcub_imagine_2290

Assessment of seasonal airborne resistome dynamics in response to air pollution exposure in the Belgrade metropolitan area (AirPollRes). in Program PROMIS. 2024;.
https://hdl.handle.net/21.15107/rcub_imagine_2290 .

"Assessment of seasonal airborne resistome dynamics in response to air pollution exposure in the Belgrade metropolitan area (AirPollRes)" in Program PROMIS (2024),
https://hdl.handle.net/21.15107/rcub_imagine_2290 .

TY  - JOUR
AU  - Tomić Vujović, Kristina
AU  - Ugrin, Milena
AU  - Tošić, Nataša
AU  - Vuković, Vojin
AU  - Marjanović, Irena
AU  - Kostić, Tatjana
AU  - Stanković, Sanja
AU  - Otasević, Vladimir
AU  - Sarać, Sofija
AU  - Antić, Darko
AU  - Pavlović, Sonja
AU  - Karan-Đurasević, Teodora
PY  - 2024
UR  - https://www.mdpi.com/1422-0067/25/2/922
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2318
AB  - Dysregulated expression of the long non-coding RNA MALAT1 has been implicated in the pathogenesis and progression of a variety of cancers, including hematological malignancies, but it has been poorly investigated in chronic lymphocytic leukemia (CLL). In this study, the expression of MALAT1 was measured using a quantitative reverse-transcriptase polymerase chain reaction in the peripheral blood mononuclear cells of 114 unselected, newly diagnosed CLL patients in order to analyze its association with clinical, laboratory, and molecular patients’ characteristics at diagnosis, as well as its prognostic relevance. MALAT1 was found to be upregulated in CLL patients in comparison to healthy controls, and expression levels were not related to age, leukocyte, lymphocyte and platelet count, serum β2-microglobulin, and IGHV somatic hypermutational status. On the other hand, high MALAT1 expression was associated with several favorable prognostic markers (high hemoglobin, low serum lactate dehydrogenase, earlier clinical stages, CD38-negative status), but also with unfavorable cytogenetics. Furthermore, an association between high MALAT1 levels and longer time to first treatment and overall survival in IGHV-unmutated CLL subtype was observed. In summary, our results imply that high MALAT1 expression at diagnosis may be a predictor of better prognosis and point to MALAT1 expression profiling as a candidate biomarker potentially useful in clinical practice.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Expression Pattern and Prognostic Significance of the Long Non-Coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 in Chronic Lymphocytic Leukemia
IS  - 2
SP  - 922
VL  - 25
DO  - 10.3390/ijms25020922
ER  - 

@article{
author = "Tomić Vujović, Kristina and Ugrin, Milena and Tošić, Nataša and Vuković, Vojin and Marjanović, Irena and Kostić, Tatjana and Stanković, Sanja and Otasević, Vladimir and Sarać, Sofija and Antić, Darko and Pavlović, Sonja and Karan-Đurasević, Teodora",
year = "2024",
abstract = "Dysregulated expression of the long non-coding RNA MALAT1 has been implicated in the pathogenesis and progression of a variety of cancers, including hematological malignancies, but it has been poorly investigated in chronic lymphocytic leukemia (CLL). In this study, the expression of MALAT1 was measured using a quantitative reverse-transcriptase polymerase chain reaction in the peripheral blood mononuclear cells of 114 unselected, newly diagnosed CLL patients in order to analyze its association with clinical, laboratory, and molecular patients’ characteristics at diagnosis, as well as its prognostic relevance. MALAT1 was found to be upregulated in CLL patients in comparison to healthy controls, and expression levels were not related to age, leukocyte, lymphocyte and platelet count, serum β2-microglobulin, and IGHV somatic hypermutational status. On the other hand, high MALAT1 expression was associated with several favorable prognostic markers (high hemoglobin, low serum lactate dehydrogenase, earlier clinical stages, CD38-negative status), but also with unfavorable cytogenetics. Furthermore, an association between high MALAT1 levels and longer time to first treatment and overall survival in IGHV-unmutated CLL subtype was observed. In summary, our results imply that high MALAT1 expression at diagnosis may be a predictor of better prognosis and point to MALAT1 expression profiling as a candidate biomarker potentially useful in clinical practice.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Expression Pattern and Prognostic Significance of the Long Non-Coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 in Chronic Lymphocytic Leukemia",
number = "2",
pages = "922",
volume = "25",
doi = "10.3390/ijms25020922"
}

Tomić Vujović, K., Ugrin, M., Tošić, N., Vuković, V., Marjanović, I., Kostić, T., Stanković, S., Otasević, V., Sarać, S., Antić, D., Pavlović, S.,& Karan-Đurasević, T.. (2024). Expression Pattern and Prognostic Significance of the Long Non-Coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 in Chronic Lymphocytic Leukemia. in International Journal of Molecular Sciences
MDPI., 25(2), 922.
https://doi.org/10.3390/ijms25020922

Tomić Vujović K, Ugrin M, Tošić N, Vuković V, Marjanović I, Kostić T, Stanković S, Otasević V, Sarać S, Antić D, Pavlović S, Karan-Đurasević T. Expression Pattern and Prognostic Significance of the Long Non-Coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 in Chronic Lymphocytic Leukemia. in International Journal of Molecular Sciences. 2024;25(2):922.
doi:10.3390/ijms25020922 .

Tomić Vujović, Kristina, Ugrin, Milena, Tošić, Nataša, Vuković, Vojin, Marjanović, Irena, Kostić, Tatjana, Stanković, Sanja, Otasević, Vladimir, Sarać, Sofija, Antić, Darko, Pavlović, Sonja, Karan-Đurasević, Teodora, "Expression Pattern and Prognostic Significance of the Long Non-Coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 in Chronic Lymphocytic Leukemia" in International Journal of Molecular Sciences, 25, no. 2 (2024):922,
https://doi.org/10.3390/ijms25020922 . .

TY  - JOUR
AU  - Pantelić, Brana
AU  - Siaperas, Romanos
AU  - Budin, Clémence
AU  - de Boer, Tjalf
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2024
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/1751-7915.14445
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2337
AB  - Global plastic waste accumulation has become omnipresent in public discourse and the focus of scientific research. Ranking as the sixth most produced polymer globally, polyurethanes (PU) significantly contribute to plastic waste and environmental pollution due to the toxicity of their building blocks, such as diisocyanates. In this study, the effects of PU on soil microbial communities over 18 months were monitored revealing that it had marginal effects on microbial diversity. However, Streptomyces sp. PU10, isolated from this PU-contaminated soil, proved exceptional in the degradation of a soluble polyester-PU (Impranil) across a range of temperatures with over 96% degradation of 10 g/L in 48 h. Proteins involved in PU degradation and metabolic changes occurring in this strain with Impranil as the sole carbon source were further investigated employing quantitative proteomics. The proposed degradation mechanism implicated the action of three enzymes: a polyester-degrading esterase, a urethane bond-degrading amidase and an oxidoreductase. Furthermore, proteome data revealed that PU degradation intermediates were incorporated into Streptomyces sp. PU10 metabolism via the fatty acid degradation pathway and subsequently channelled to polyketide biosynthesis. Most notably, the production of the tri-pyrrole undecylprodigiosin was confirmed paving the way for establishing PU upcycling strategies to bioactive metabolites using Streptomyces strains.
PB  - Wiley
T2  - Microbial Biotechnology
T2  - Microbial Biotechnology
T1  - Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production
IS  - 3
SP  - e14445
VL  - 17
DO  - 10.1111/1751-7915.14445
ER  - 

@article{
author = "Pantelić, Brana and Siaperas, Romanos and Budin, Clémence and de Boer, Tjalf and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2024",
abstract = "Global plastic waste accumulation has become omnipresent in public discourse and the focus of scientific research. Ranking as the sixth most produced polymer globally, polyurethanes (PU) significantly contribute to plastic waste and environmental pollution due to the toxicity of their building blocks, such as diisocyanates. In this study, the effects of PU on soil microbial communities over 18 months were monitored revealing that it had marginal effects on microbial diversity. However, Streptomyces sp. PU10, isolated from this PU-contaminated soil, proved exceptional in the degradation of a soluble polyester-PU (Impranil) across a range of temperatures with over 96% degradation of 10 g/L in 48 h. Proteins involved in PU degradation and metabolic changes occurring in this strain with Impranil as the sole carbon source were further investigated employing quantitative proteomics. The proposed degradation mechanism implicated the action of three enzymes: a polyester-degrading esterase, a urethane bond-degrading amidase and an oxidoreductase. Furthermore, proteome data revealed that PU degradation intermediates were incorporated into Streptomyces sp. PU10 metabolism via the fatty acid degradation pathway and subsequently channelled to polyketide biosynthesis. Most notably, the production of the tri-pyrrole undecylprodigiosin was confirmed paving the way for establishing PU upcycling strategies to bioactive metabolites using Streptomyces strains.",
publisher = "Wiley",
journal = "Microbial Biotechnology, Microbial Biotechnology",
title = "Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production",
number = "3",
pages = "e14445",
volume = "17",
doi = "10.1111/1751-7915.14445"
}

Pantelić, B., Siaperas, R., Budin, C., de Boer, T., Topakas, E.,& Nikodinović-Runić, J.. (2024). Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production. in Microbial Biotechnology
Wiley., 17(3), e14445.
https://doi.org/10.1111/1751-7915.14445

Pantelić B, Siaperas R, Budin C, de Boer T, Topakas E, Nikodinović-Runić J. Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production. in Microbial Biotechnology. 2024;17(3):e14445.
doi:10.1111/1751-7915.14445 .

Pantelić, Brana, Siaperas, Romanos, Budin, Clémence, de Boer, Tjalf, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production" in Microbial Biotechnology, 17, no. 3 (2024):e14445,
https://doi.org/10.1111/1751-7915.14445 . .

TY  - JOUR
AU  - Babić Radić, Marija
AU  - Vukomanović, Marija
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida
PY  - 2024
UR  - https://www.mdpi.com/1999-4923/16/3/372
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2330
AB  - This study proposes synthesis and evaluation of gelatin-/alginate-based hydrogel scaffolds reinforced with titanium dioxide (TiO2) nanoparticles which, through their combination with allantoin, quercetin, and caffeic acid, provide multi-target therapy directed on all phases of the wound healing process. These scaffolds provide the simultaneous release of bioactive agents and concurrently support cell/tissue repair through the replicated structure of a native extracellular matrix. The hydrogel scaffolds were synthesized via a crosslinking reaction using EDC as a crosslinker for gelatin. Synthesized hydrogel scaffolds and the effect of TiO2 on their properties were characterized by structural, mechanical, morphological, and swelling properties, and the porosity, wettability, adhesion to skin tissue, and simultaneous release features. The biocompatibility of the scaffolds was tested in vitro on fibroblasts (MRC5 cells) and in vivo (Caenorhabditis elegans) in a survival probe. The scaffolds revealed porous interconnected morphology, porosity of 88.33 to 96.76%, elastic modulus of 1.53 to 4.29 MPa, full hydrophilicity, favorable skin adhesivity, and biocompatibility. The simultaneous release was investigated in vitro indicating dependence on the scaffold’s composition and type of bioactive agents. The novel scaffolds designed as multi-target therapy have significant promise for improved wound healing in a beneficial and non-invasive manner.
PB  - MDPI
T2  - Pharmaceutics
T2  - Pharmaceutics
T1  - Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO2 Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform
IS  - 3
SP  - 372
VL  - 16
DO  - 10.3390/pharmaceutics16030372
ER  - 

@article{
author = "Babić Radić, Marija and Vukomanović, Marija and Nikodinović-Runić, Jasmina and Tomić, Simonida",
year = "2024",
abstract = "This study proposes synthesis and evaluation of gelatin-/alginate-based hydrogel scaffolds reinforced with titanium dioxide (TiO2) nanoparticles which, through their combination with allantoin, quercetin, and caffeic acid, provide multi-target therapy directed on all phases of the wound healing process. These scaffolds provide the simultaneous release of bioactive agents and concurrently support cell/tissue repair through the replicated structure of a native extracellular matrix. The hydrogel scaffolds were synthesized via a crosslinking reaction using EDC as a crosslinker for gelatin. Synthesized hydrogel scaffolds and the effect of TiO2 on their properties were characterized by structural, mechanical, morphological, and swelling properties, and the porosity, wettability, adhesion to skin tissue, and simultaneous release features. The biocompatibility of the scaffolds was tested in vitro on fibroblasts (MRC5 cells) and in vivo (Caenorhabditis elegans) in a survival probe. The scaffolds revealed porous interconnected morphology, porosity of 88.33 to 96.76%, elastic modulus of 1.53 to 4.29 MPa, full hydrophilicity, favorable skin adhesivity, and biocompatibility. The simultaneous release was investigated in vitro indicating dependence on the scaffold’s composition and type of bioactive agents. The novel scaffolds designed as multi-target therapy have significant promise for improved wound healing in a beneficial and non-invasive manner.",
publisher = "MDPI",
journal = "Pharmaceutics, Pharmaceutics",
title = "Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO2 Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform",
number = "3",
pages = "372",
volume = "16",
doi = "10.3390/pharmaceutics16030372"
}

Babić Radić, M., Vukomanović, M., Nikodinović-Runić, J.,& Tomić, S.. (2024). Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO2 Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform. in Pharmaceutics
MDPI., 16(3), 372.
https://doi.org/10.3390/pharmaceutics16030372

Babić Radić M, Vukomanović M, Nikodinović-Runić J, Tomić S. Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO2 Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform. in Pharmaceutics. 2024;16(3):372.
doi:10.3390/pharmaceutics16030372 .

Babić Radić, Marija, Vukomanović, Marija, Nikodinović-Runić, Jasmina, Tomić, Simonida, "Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO2 Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform" in Pharmaceutics, 16, no. 3 (2024):372,
https://doi.org/10.3390/pharmaceutics16030372 . .

TY  - CONF
AU  - Ćurčić, Jovana
AU  - Malešević, Milka
AU  - Jovčić, Branko
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1308
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2359
AB  - Biofilm-associated infections are the main cause of biomaterial implant failure today. The increasing prevalence of antibiotic-resistant pathogens often results in the only solution of implant movement, with serious consequences for patients. Recently, various antimicrobial agents have been recognized as a promising strategy to prevent biofilm formation on implant surfaces. Quorum sensing (QS) plays a central role in the control of bacterial virulence and biofilm formation. The use of quorum quenching (QQ) enzymes to target QS is therefore a promising innovative approach for the development of enzyme-based antivirulence therapeutics, which represent a potential solution to combat infections caused by multidrug-resistant pathogens. This study aimed to characterize the novel YtnP lactonase from the clinical isolate Stenotrophomonas maltophilia 6960 and to investigate its potential to combat the virulence of multidrug-resistant (MDR) Pseudomonas aeruginosa MMA83.
C3  - Hemijska industrija (Chemical Industry)
T1  - A novel thermostable YtnP lactonase inhibits biofilm formation and induces decomposition of preformed Pseudomonas aeruginosa biofilms
EP  - 61
IS  - 1S
SP  - 61
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2359
ER  - 

@conference{
author = "Ćurčić, Jovana and Malešević, Milka and Jovčić, Branko",
year = "2024",
abstract = "Biofilm-associated infections are the main cause of biomaterial implant failure today. The increasing prevalence of antibiotic-resistant pathogens often results in the only solution of implant movement, with serious consequences for patients. Recently, various antimicrobial agents have been recognized as a promising strategy to prevent biofilm formation on implant surfaces. Quorum sensing (QS) plays a central role in the control of bacterial virulence and biofilm formation. The use of quorum quenching (QQ) enzymes to target QS is therefore a promising innovative approach for the development of enzyme-based antivirulence therapeutics, which represent a potential solution to combat infections caused by multidrug-resistant pathogens. This study aimed to characterize the novel YtnP lactonase from the clinical isolate Stenotrophomonas maltophilia 6960 and to investigate its potential to combat the virulence of multidrug-resistant (MDR) Pseudomonas aeruginosa MMA83.",
journal = "Hemijska industrija (Chemical Industry)",
title = "A novel thermostable YtnP lactonase inhibits biofilm formation and induces decomposition of preformed Pseudomonas aeruginosa biofilms",
pages = "61-61",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2359"
}

Ćurčić, J., Malešević, M.,& Jovčić, B.. (2024). A novel thermostable YtnP lactonase inhibits biofilm formation and induces decomposition of preformed Pseudomonas aeruginosa biofilms. in Hemijska industrija (Chemical Industry), 78(1S), 61-61.
https://hdl.handle.net/21.15107/rcub_imagine_2359

Ćurčić J, Malešević M, Jovčić B. A novel thermostable YtnP lactonase inhibits biofilm formation and induces decomposition of preformed Pseudomonas aeruginosa biofilms. in Hemijska industrija (Chemical Industry). 2024;78(1S):61-61.
https://hdl.handle.net/21.15107/rcub_imagine_2359 .

Ćurčić, Jovana, Malešević, Milka, Jovčić, Branko, "A novel thermostable YtnP lactonase inhibits biofilm formation and induces decomposition of preformed Pseudomonas aeruginosa biofilms" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):61-61,
https://hdl.handle.net/21.15107/rcub_imagine_2359 .

TY  - JOUR
AU  - Ćurčić, Jovana
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Vasiljević, Zorica
AU  - Kojić, Milan
AU  - Jovčić, Branko
AU  - Malešević, Milka
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0141813024012248
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2326
AB  - Infections caused by multidrug-resistant pathogens are one of the biggest challenges facing the healthcare system today. Quorum quenching (QQ) enzymes have the potential to be used as innovative enzyme-based antivirulence therapeutics to combat infections caused by multidrug-resistant pathogens. The main objective of this research was to describe the novel YtnP lactonase derived from the clinical isolate Stenotrophomonas maltophilia and to investigate its antivirulence potential against multidrug-resistant Pseudomonas aeruginosa MMA83. YtnP lactonase, the QQ enzyme, belongs to the family of metallo-β-lactamases. The recombinant enzyme has several advantageous biotechnological properties, such as high thermostability, activity in a wide pH range, and no cytotoxic effect. High-performance liquid chromatography analysis revealed the activity of recombinant YtnP lactonase toward a wide range of N-acyl-homoserine lactones (AHLs), quorum sensing signaling molecules, with a higher preference for long-chain AHLs. Recombinant YtnP lactonase was shown to inhibit P. aeruginosa MMA83 biofilm formation, induce biofilm decomposition, and reduce extracellular virulence factors production. Moreover, the lifespan of MMA83-infected Caenorhabditis elegans was prolonged with YtnP lactonase treatment. YtnP lactonase showed synergistic inhibitory activity in combination with gentamicin and acted additively with meropenem against MMA83. The described properties make YtnP lactonase a promising therapeutic candidate for the development of next-generation antivirulence agents.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T2  - International Journal of Biological MacromoleculesInternational Journal of Biological Macromolecules
T1  - A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo
SP  - 130421
DO  - 10.1016/j.ijbiomac.2024.130421
ER  - 

@article{
author = "Ćurčić, Jovana and Dinić, Miroslav and Novović, Katarina and Vasiljević, Zorica and Kojić, Milan and Jovčić, Branko and Malešević, Milka",
year = "2024",
abstract = "Infections caused by multidrug-resistant pathogens are one of the biggest challenges facing the healthcare system today. Quorum quenching (QQ) enzymes have the potential to be used as innovative enzyme-based antivirulence therapeutics to combat infections caused by multidrug-resistant pathogens. The main objective of this research was to describe the novel YtnP lactonase derived from the clinical isolate Stenotrophomonas maltophilia and to investigate its antivirulence potential against multidrug-resistant Pseudomonas aeruginosa MMA83. YtnP lactonase, the QQ enzyme, belongs to the family of metallo-β-lactamases. The recombinant enzyme has several advantageous biotechnological properties, such as high thermostability, activity in a wide pH range, and no cytotoxic effect. High-performance liquid chromatography analysis revealed the activity of recombinant YtnP lactonase toward a wide range of N-acyl-homoserine lactones (AHLs), quorum sensing signaling molecules, with a higher preference for long-chain AHLs. Recombinant YtnP lactonase was shown to inhibit P. aeruginosa MMA83 biofilm formation, induce biofilm decomposition, and reduce extracellular virulence factors production. Moreover, the lifespan of MMA83-infected Caenorhabditis elegans was prolonged with YtnP lactonase treatment. YtnP lactonase showed synergistic inhibitory activity in combination with gentamicin and acted additively with meropenem against MMA83. The described properties make YtnP lactonase a promising therapeutic candidate for the development of next-generation antivirulence agents.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules, International Journal of Biological MacromoleculesInternational Journal of Biological Macromolecules",
title = "A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo",
pages = "130421",
doi = "10.1016/j.ijbiomac.2024.130421"
}

Ćurčić, J., Dinić, M., Novović, K., Vasiljević, Z., Kojić, M., Jovčić, B.,& Malešević, M.. (2024). A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo. in International Journal of Biological Macromolecules
Elsevier., 130421.
https://doi.org/10.1016/j.ijbiomac.2024.130421

Ćurčić J, Dinić M, Novović K, Vasiljević Z, Kojić M, Jovčić B, Malešević M. A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo. in International Journal of Biological Macromolecules. 2024;:130421.
doi:10.1016/j.ijbiomac.2024.130421 .

Ćurčić, Jovana, Dinić, Miroslav, Novović, Katarina, Vasiljević, Zorica, Kojić, Milan, Jovčić, Branko, Malešević, Milka, "A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo" in International Journal of Biological Macromolecules (2024):130421,
https://doi.org/10.1016/j.ijbiomac.2024.130421 . .

TY  - JOUR
AU  - Araujo, Jeovan A.
AU  - Taxeidis, George
AU  - Pereira, Everton H.
AU  - Azeem, Muhammad
AU  - Pantelić, Brana
AU  - Jeremić, Sanja
AU  - Ponjavić, Marijana
AU  - Chen, Yuanyuan
AU  - Mojicević, Marija
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
AU  - Brennan Fournet, Margaret
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0959652624004724
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2315
AB  - Ubiquitous post-consumer plastic waste is often physically mixed combining recalcitrant petroleum-based plastics with bioplastics, forming (petro-bio)plastic streams. Finding appropriate end-of-life (EoL) strategies for mixed (petro-bio)plastic waste is highly pertinent in achieving environmental protection, sustainability for plastic value chain industries including recyclers and government policy makers worldwide. The presence of bioplastic mixed in with polyethylene terephthalate (PET) or other petroleum-based plastic streams poses a substantial drawback to mechanical recycling and strongly impedes the development of sustainable EoL routes. Here, we present a model system for the sustainable management of mixed (petro-bio)plastic waste, demonstrating a biotechnological route through synergy-promoted enzymatic degradation of PET–representing petrochemical polyester plastic–mixed with thermoplastic starch (TPS)–as a model bioplastic. Leaf-branch compost cutinase (LCCICCG) and commercial amylase (AMY) deliver effective depolymerization of this mixed (petro-bio)plastic material, with subsequent bio-upcycling of the mixed waste stream into bacterial nanocellulose (BNC) by Komagataeibacter medellinensis. Compared to LCCICCG and AMY, the LCCICCG/AMY combined treatment synergistically produced a 2.6- and 4.4-fold increase in enzymatic decomposition at 70 °C in four days, respectively, yielding sugars and terephthalic acid (TPA) as the main depolymerization building blocks. Bio-upcycling of post-enzymatic degradation hydrolysates resulted in a high BNC yield of 3 g L−1 after 10 days. This work paves the way for sustainable management routes for challenging mixed recalcitrant plastic and bioplastic waste and prepares opportunities for its participation in the circular production of sustainable eco-polymers.
PB  - Elsevier
T2  - Journal of Cleaner Production
T1  - Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose
SP  - 141025
DO  - 10.1016/j.jclepro.2024.141025
ER  - 

@article{
author = "Araujo, Jeovan A. and Taxeidis, George and Pereira, Everton H. and Azeem, Muhammad and Pantelić, Brana and Jeremić, Sanja and Ponjavić, Marijana and Chen, Yuanyuan and Mojicević, Marija and Nikodinović-Runić, Jasmina and Topakas, Evangelos and Brennan Fournet, Margaret",
year = "2024",
abstract = "Ubiquitous post-consumer plastic waste is often physically mixed combining recalcitrant petroleum-based plastics with bioplastics, forming (petro-bio)plastic streams. Finding appropriate end-of-life (EoL) strategies for mixed (petro-bio)plastic waste is highly pertinent in achieving environmental protection, sustainability for plastic value chain industries including recyclers and government policy makers worldwide. The presence of bioplastic mixed in with polyethylene terephthalate (PET) or other petroleum-based plastic streams poses a substantial drawback to mechanical recycling and strongly impedes the development of sustainable EoL routes. Here, we present a model system for the sustainable management of mixed (petro-bio)plastic waste, demonstrating a biotechnological route through synergy-promoted enzymatic degradation of PET–representing petrochemical polyester plastic–mixed with thermoplastic starch (TPS)–as a model bioplastic. Leaf-branch compost cutinase (LCCICCG) and commercial amylase (AMY) deliver effective depolymerization of this mixed (petro-bio)plastic material, with subsequent bio-upcycling of the mixed waste stream into bacterial nanocellulose (BNC) by Komagataeibacter medellinensis. Compared to LCCICCG and AMY, the LCCICCG/AMY combined treatment synergistically produced a 2.6- and 4.4-fold increase in enzymatic decomposition at 70 °C in four days, respectively, yielding sugars and terephthalic acid (TPA) as the main depolymerization building blocks. Bio-upcycling of post-enzymatic degradation hydrolysates resulted in a high BNC yield of 3 g L−1 after 10 days. This work paves the way for sustainable management routes for challenging mixed recalcitrant plastic and bioplastic waste and prepares opportunities for its participation in the circular production of sustainable eco-polymers.",
publisher = "Elsevier",
journal = "Journal of Cleaner Production",
title = "Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose",
pages = "141025",
doi = "10.1016/j.jclepro.2024.141025"
}

Araujo, J. A., Taxeidis, G., Pereira, E. H., Azeem, M., Pantelić, B., Jeremić, S., Ponjavić, M., Chen, Y., Mojicević, M., Nikodinović-Runić, J., Topakas, E.,& Brennan Fournet, M.. (2024). Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose. in Journal of Cleaner Production
Elsevier., 141025.
https://doi.org/10.1016/j.jclepro.2024.141025

Araujo JA, Taxeidis G, Pereira EH, Azeem M, Pantelić B, Jeremić S, Ponjavić M, Chen Y, Mojicević M, Nikodinović-Runić J, Topakas E, Brennan Fournet M. Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose. in Journal of Cleaner Production. 2024;:141025.
doi:10.1016/j.jclepro.2024.141025 .

Araujo, Jeovan A., Taxeidis, George, Pereira, Everton H., Azeem, Muhammad, Pantelić, Brana, Jeremić, Sanja, Ponjavić, Marijana, Chen, Yuanyuan, Mojicević, Marija, Nikodinović-Runić, Jasmina, Topakas, Evangelos, Brennan Fournet, Margaret, "Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose" in Journal of Cleaner Production (2024):141025,
https://doi.org/10.1016/j.jclepro.2024.141025 . .

TY  - JOUR
AU  - Miloradović, Zorana
AU  - Kovačević, Jovana
AU  - Miočionović, Jelena
AU  - Đekić, Ilija
AU  - Kljajević, Nemanja
AU  - Smigić, Nada
PY  - 2024
UR  - https://www.cell.com/heliyon/abstract/S2405-8440(24)03473-X
UR  - http://www.ncbi.nlm.nih.gov/pubmed/38187278
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2343
AB  - The objective of this study was to identify the requirements needed for selling dairy products through e-commerce, as well as current gaps and challenges that exist for small scale dairy processors (SSDPs), and need to be addressed in order to comply with those requirements. A mixed method research design was used for training needs assessment. Qualitative (in-depth interview with 7 online platform representatives (OPRs)) and quantitative approach (survey questionnaire with 58 SSDPs) were conducted. Interview transcripts were coded and codes were grouped into seven themes. Hierarchical cluster analysis was applied to 146 answers from 58 SSDPs. They were divided into 4 clusters. Mean sums of responses between clusters were compared by Mann-Whitney U test. OPRs suggested that SSDPs should be provided with tools and resources to help them achieve food safety and quality targets, as well as practical knowledge and skills. They reported that it is crucial to find a solution for the cold chain transportation, for maintaining consistent product quality. Survey results showed that SSDPs use kitchen equipment (79.3%) and kitchen cleaning products (81.0%) for dairy processing. In total, 43.1% process raw milk and only 24.1% have product label on the package. Only members of cluster 3 and 4 sell their products online (73.7% and 90.0%, respectively), mostly using their own social media platforms (57.9% and 60.0%, respectively), transporting products to end buyers by themselves in hand refrigerators (47.4% and 70.0%, respectively). By analyzing the differences among clusters of SSDPs, trainings can be tailored to the characteristics and knowledge gaps of each group.
PB  - CellPress
T2  - Heliyon
T2  - HeliyonHeliyon
T1  - E-commerce readiness and training needs of small-scale dairy processors in Serbia: Understanding barriers and knowledge gaps
IS  - 6
VL  - 10
DO  - 10.1016/j.heliyon.2024.e27442
ER  - 

@article{
author = "Miloradović, Zorana and Kovačević, Jovana and Miočionović, Jelena and Đekić, Ilija and Kljajević, Nemanja and Smigić, Nada",
year = "2024",
abstract = "The objective of this study was to identify the requirements needed for selling dairy products through e-commerce, as well as current gaps and challenges that exist for small scale dairy processors (SSDPs), and need to be addressed in order to comply with those requirements. A mixed method research design was used for training needs assessment. Qualitative (in-depth interview with 7 online platform representatives (OPRs)) and quantitative approach (survey questionnaire with 58 SSDPs) were conducted. Interview transcripts were coded and codes were grouped into seven themes. Hierarchical cluster analysis was applied to 146 answers from 58 SSDPs. They were divided into 4 clusters. Mean sums of responses between clusters were compared by Mann-Whitney U test. OPRs suggested that SSDPs should be provided with tools and resources to help them achieve food safety and quality targets, as well as practical knowledge and skills. They reported that it is crucial to find a solution for the cold chain transportation, for maintaining consistent product quality. Survey results showed that SSDPs use kitchen equipment (79.3%) and kitchen cleaning products (81.0%) for dairy processing. In total, 43.1% process raw milk and only 24.1% have product label on the package. Only members of cluster 3 and 4 sell their products online (73.7% and 90.0%, respectively), mostly using their own social media platforms (57.9% and 60.0%, respectively), transporting products to end buyers by themselves in hand refrigerators (47.4% and 70.0%, respectively). By analyzing the differences among clusters of SSDPs, trainings can be tailored to the characteristics and knowledge gaps of each group.",
publisher = "CellPress",
journal = "Heliyon, HeliyonHeliyon",
title = "E-commerce readiness and training needs of small-scale dairy processors in Serbia: Understanding barriers and knowledge gaps",
number = "6",
volume = "10",
doi = "10.1016/j.heliyon.2024.e27442"
}

Miloradović, Z., Kovačević, J., Miočionović, J., Đekić, I., Kljajević, N.,& Smigić, N.. (2024). E-commerce readiness and training needs of small-scale dairy processors in Serbia: Understanding barriers and knowledge gaps. in Heliyon
CellPress., 10(6).
https://doi.org/10.1016/j.heliyon.2024.e27442

Miloradović Z, Kovačević J, Miočionović J, Đekić I, Kljajević N, Smigić N. E-commerce readiness and training needs of small-scale dairy processors in Serbia: Understanding barriers and knowledge gaps. in Heliyon. 2024;10(6).
doi:10.1016/j.heliyon.2024.e27442 .

Miloradović, Zorana, Kovačević, Jovana, Miočionović, Jelena, Đekić, Ilija, Kljajević, Nemanja, Smigić, Nada, "E-commerce readiness and training needs of small-scale dairy processors in Serbia: Understanding barriers and knowledge gaps" in Heliyon, 10, no. 6 (2024),
https://doi.org/10.1016/j.heliyon.2024.e27442 . .