TY  - JOUR
AU  - Spasovski, Vesna
AU  - Romolo, Anna
AU  - Zagorc, Urška
AU  - Arrigler, Vesna
AU  - Kisovec, Matic
AU  - Zavec, Apolonija Bedina
AU  - Arko, Matevž
AU  - Molnár, Adrienn
AU  - Schlosser, Gitta
AU  - Iglič, Aleš
AU  - Kogej, Ksenija
AU  - Kralj-Iglič, Veronika
PY  - 2024
UR  - https://www.dovepress.com/characterization-of-nanohybridosomes-from-lipids-and-spruce-homogenate-peer-reviewed-fulltext-article-IJN
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2332
AB  - Introduction: Lipid nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called nano-sized
hybridosomes or nanohybridosomes, NSHs) were considered.
Methods: We formed NSHs by mixing appropriate amounts of lecithin, glycerol and supernatant of isolation of extracellular vesicles
from spruce needle homogenate. We visualized NSHs by light microscopy and cryogenic transmission electron microscopy and
assessed them by flow cytometry, dynamic light scattering, ultraviolet–visual spectroscopy, interferometric light microscopy and liquid
chromatography–mass spectrometry.
Results: We found that the particles consisted of a bilayer membrane and a fluid-like interior. Flow cytometry and
interferometric light microscopy measurements showed that the majority of the particles were nano-sized. Dynamic light
scattering and interferometric light microscopy measurements agreed well on the average hydrodynamic radius of the
particles Rh (between 140 and 180 nm), while the concentrations of the particles were in the range between 1013 and
1014/mL indicating that NSHs present a considerable (more than 25%) of the sample which is much more than the yield of
natural extracellular vesicles (EVs) from spruce needle homogenate (estimated less than 1%). Spruce specific lipids and
proteins were found in hybridosomes.
Discussion: Simple and low-cost preparation method, non-demanding saving process and efficient formation procedure suggest that
large-scale production of NSHs from lipids and spruce needle homogenate is feasible.
Plain Language Summary: Cells shed into their exterior nanoparticles (here referred to as extracellular vesicles – EVs) that
are free to move, reach distant cells and are taken up by them. As they carry bioactive constituents, EVs may have important
impact on the recipient cells. The mechanisms of EV formation and mediation can be employed in designing therapeutic,
prophylactic and diagnostic methods for various medical issues. EVs can be harvested from biological samples; however,
their yield is small,12 and there are potential side effects. Artificial vesicles – liposomes – have high yield; however, in vivo,
they can be degraded before reaching the target and their reproducibility is yet insufficient. In order to combine advantages of
both types of nanoparticles, we have composed nanohybridosomes (NSHs) from soya lecithin, water and supernatant of
isolation of EVs from spruce needle homogenate, visualized them by cryogenic electron microscopy and characterized them
with respect to their size, concentration and protein/nucleic acid content. We have applied a recently developed interferometric light microscopy to determine the hydrodynamic radius and the concentration of EVs. We found that the majority of
composed particles are nano-sized and that they enclose more than 25% of the incoming volume of liquid, which is considerably more than about 1% that can be harvested by isolation of EVs from spruce needle homogenate by (ultra)
centrifugation
PB  - Dove Press Ltd
T2  - International Journal of Nanomedicine
T1  - Characterization of Nanohybridosomes from Lipids and Spruce Homogenate Containing Extracellular Vesicles
EP  - 1721
SP  - 1709
VL  - 19
DO  - 10.2147/IJN.S432836
ER  - 

@article{
author = "Spasovski, Vesna and Romolo, Anna and Zagorc, Urška and Arrigler, Vesna and Kisovec, Matic and Zavec, Apolonija Bedina and Arko, Matevž and Molnár, Adrienn and Schlosser, Gitta and Iglič, Aleš and Kogej, Ksenija and Kralj-Iglič, Veronika",
year = "2024",
abstract = "Introduction: Lipid nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called nano-sized
hybridosomes or nanohybridosomes, NSHs) were considered.
Methods: We formed NSHs by mixing appropriate amounts of lecithin, glycerol and supernatant of isolation of extracellular vesicles
from spruce needle homogenate. We visualized NSHs by light microscopy and cryogenic transmission electron microscopy and
assessed them by flow cytometry, dynamic light scattering, ultraviolet–visual spectroscopy, interferometric light microscopy and liquid
chromatography–mass spectrometry.
Results: We found that the particles consisted of a bilayer membrane and a fluid-like interior. Flow cytometry and
interferometric light microscopy measurements showed that the majority of the particles were nano-sized. Dynamic light
scattering and interferometric light microscopy measurements agreed well on the average hydrodynamic radius of the
particles Rh (between 140 and 180 nm), while the concentrations of the particles were in the range between 1013 and
1014/mL indicating that NSHs present a considerable (more than 25%) of the sample which is much more than the yield of
natural extracellular vesicles (EVs) from spruce needle homogenate (estimated less than 1%). Spruce specific lipids and
proteins were found in hybridosomes.
Discussion: Simple and low-cost preparation method, non-demanding saving process and efficient formation procedure suggest that
large-scale production of NSHs from lipids and spruce needle homogenate is feasible.
Plain Language Summary: Cells shed into their exterior nanoparticles (here referred to as extracellular vesicles – EVs) that
are free to move, reach distant cells and are taken up by them. As they carry bioactive constituents, EVs may have important
impact on the recipient cells. The mechanisms of EV formation and mediation can be employed in designing therapeutic,
prophylactic and diagnostic methods for various medical issues. EVs can be harvested from biological samples; however,
their yield is small,12 and there are potential side effects. Artificial vesicles – liposomes – have high yield; however, in vivo,
they can be degraded before reaching the target and their reproducibility is yet insufficient. In order to combine advantages of
both types of nanoparticles, we have composed nanohybridosomes (NSHs) from soya lecithin, water and supernatant of
isolation of EVs from spruce needle homogenate, visualized them by cryogenic electron microscopy and characterized them
with respect to their size, concentration and protein/nucleic acid content. We have applied a recently developed interferometric light microscopy to determine the hydrodynamic radius and the concentration of EVs. We found that the majority of
composed particles are nano-sized and that they enclose more than 25% of the incoming volume of liquid, which is considerably more than about 1% that can be harvested by isolation of EVs from spruce needle homogenate by (ultra)
centrifugation",
publisher = "Dove Press Ltd",
journal = "International Journal of Nanomedicine",
title = "Characterization of Nanohybridosomes from Lipids and Spruce Homogenate Containing Extracellular Vesicles",
pages = "1721-1709",
volume = "19",
doi = "10.2147/IJN.S432836"
}

Spasovski, V., Romolo, A., Zagorc, U., Arrigler, V., Kisovec, M., Zavec, A. B., Arko, M., Molnár, A., Schlosser, G., Iglič, A., Kogej, K.,& Kralj-Iglič, V.. (2024). Characterization of Nanohybridosomes from Lipids and Spruce Homogenate Containing Extracellular Vesicles. in International Journal of Nanomedicine
Dove Press Ltd., 19, 1709-1721.
https://doi.org/10.2147/IJN.S432836

Spasovski V, Romolo A, Zagorc U, Arrigler V, Kisovec M, Zavec AB, Arko M, Molnár A, Schlosser G, Iglič A, Kogej K, Kralj-Iglič V. Characterization of Nanohybridosomes from Lipids and Spruce Homogenate Containing Extracellular Vesicles. in International Journal of Nanomedicine. 2024;19:1709-1721.
doi:10.2147/IJN.S432836 .

Spasovski, Vesna, Romolo, Anna, Zagorc, Urška, Arrigler, Vesna, Kisovec, Matic, Zavec, Apolonija Bedina, Arko, Matevž, Molnár, Adrienn, Schlosser, Gitta, Iglič, Aleš, Kogej, Ksenija, Kralj-Iglič, Veronika, "Characterization of Nanohybridosomes from Lipids and Spruce Homogenate Containing Extracellular Vesicles" in International Journal of Nanomedicine, 19 (2024):1709-1721,
https://doi.org/10.2147/IJN.S432836 . .

TY  - JOUR
AU  - Jeran, Marko
AU  - Romolo, Anna
AU  - Spasovski, Vesna
AU  - Hočevar, Matej
AU  - Novak, Urban
AU  - Štukelj, Roman
AU  - Šuštar, Vid
AU  - Kisovec, Matic
AU  - Bedina Zavec, Apolonija
AU  - Kogej, Ksenija
AU  - Iglič, Aleš
AU  - Trebše, Polonca
AU  - Kralj-Iglič, Veronika
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2086
AB  - Small cellular particles (SCPs) are being considered for their role in cell-to-cell communication. We harvested and characterized SCPs from spruce needle homogenate. SCPs were isolated by differential ultracentrifugation. They were imaged by scanning electron microscope (SEM) and cryogenic transmission electron microscope (cryo TEM), assessed for their number density and hydrodynamic diameter by interferometric light microscopy (ILM) and flow cytometry (FCM), total phenolic content (TPC) by UV-vis spectroscopy, and terpene content by gas chromatography-mass spectrometry (GC-MS). The supernatant after ultracentrifugation at 50,000× g contained bilayer-enclosed vesicles whereas in the isolate we observed small particles of other types and only a few vesicles. The number density of cell-sized particles (CSPs) (larger than 2 μm) and meso-sized particles (MSPs) (cca 400 nm–2 µm) was about four orders of magnitude lower than the number density of SCPs (sized below 500 nm). The average hydrodynamic diameter of SCPs measured in 10,029 SCPs was 161 ± 133 nm. TCP decreased considerably due to 5-day aging. Volatile terpenoid content was found in the pellet after 300× g. The above results indicate that spruce needle homogenate is a source of vesicles to be explored for potential delivery use.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Small Cellular Particles from European Spruce Needle Homogenate
IS  - 5
SP  - 4349
VL  - 24
DO  - 10.3390/ijms24054349
ER  - 

@article{
author = "Jeran, Marko and Romolo, Anna and Spasovski, Vesna and Hočevar, Matej and Novak, Urban and Štukelj, Roman and Šuštar, Vid and Kisovec, Matic and Bedina Zavec, Apolonija and Kogej, Ksenija and Iglič, Aleš and Trebše, Polonca and Kralj-Iglič, Veronika",
year = "2023",
abstract = "Small cellular particles (SCPs) are being considered for their role in cell-to-cell communication. We harvested and characterized SCPs from spruce needle homogenate. SCPs were isolated by differential ultracentrifugation. They were imaged by scanning electron microscope (SEM) and cryogenic transmission electron microscope (cryo TEM), assessed for their number density and hydrodynamic diameter by interferometric light microscopy (ILM) and flow cytometry (FCM), total phenolic content (TPC) by UV-vis spectroscopy, and terpene content by gas chromatography-mass spectrometry (GC-MS). The supernatant after ultracentrifugation at 50,000× g contained bilayer-enclosed vesicles whereas in the isolate we observed small particles of other types and only a few vesicles. The number density of cell-sized particles (CSPs) (larger than 2 μm) and meso-sized particles (MSPs) (cca 400 nm–2 µm) was about four orders of magnitude lower than the number density of SCPs (sized below 500 nm). The average hydrodynamic diameter of SCPs measured in 10,029 SCPs was 161 ± 133 nm. TCP decreased considerably due to 5-day aging. Volatile terpenoid content was found in the pellet after 300× g. The above results indicate that spruce needle homogenate is a source of vesicles to be explored for potential delivery use.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Small Cellular Particles from European Spruce Needle Homogenate",
number = "5",
pages = "4349",
volume = "24",
doi = "10.3390/ijms24054349"
}

Jeran, M., Romolo, A., Spasovski, V., Hočevar, M., Novak, U., Štukelj, R., Šuštar, V., Kisovec, M., Bedina Zavec, A., Kogej, K., Iglič, A., Trebše, P.,& Kralj-Iglič, V.. (2023). Small Cellular Particles from European Spruce Needle Homogenate. in International Journal of Molecular Sciences
MDPI., 24(5), 4349.
https://doi.org/10.3390/ijms24054349

Jeran M, Romolo A, Spasovski V, Hočevar M, Novak U, Štukelj R, Šuštar V, Kisovec M, Bedina Zavec A, Kogej K, Iglič A, Trebše P, Kralj-Iglič V. Small Cellular Particles from European Spruce Needle Homogenate. in International Journal of Molecular Sciences. 2023;24(5):4349.
doi:10.3390/ijms24054349 .

Jeran, Marko, Romolo, Anna, Spasovski, Vesna, Hočevar, Matej, Novak, Urban, Štukelj, Roman, Šuštar, Vid, Kisovec, Matic, Bedina Zavec, Apolonija, Kogej, Ksenija, Iglič, Aleš, Trebše, Polonca, Kralj-Iglič, Veronika, "Small Cellular Particles from European Spruce Needle Homogenate" in International Journal of Molecular Sciences, 24, no. 5 (2023):4349,
https://doi.org/10.3390/ijms24054349 . .

TY  - CONF
AU  - Spasovski, Vesna
AU  - Romolo, Anna
AU  - Kisovec, Matic
AU  - Zagorc, Urška
AU  - Arrigler, Vesna
AU  - Arko, Matevž
AU  - Bedina Zavec, Apolonija
AU  - Iglič, Aleš
AU  - Kogej, Ksenija
AU  - Kralj-Iglič, Veronika
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2265
AB  - Introduction: Being of compatible structure with biomembranes, lipid–based nanoparticles are considered
as convenient platforms for drug delivery systems. In the proposed work we considered formation of lipid
nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called
hybridosomes). We formed hybridosomes by mixing appropriate amounts of lecithin, supernatant of isolation
of extracellular particles from spruce needle homogenate and glycerol.
Methods: We visualized hybridosomes by light microscopy and cryogenic transmission electron microscopy
and assessed them by flow cytometry, dynamic light scattering, ultraviolet–visual spectroscopy and
interferometric microscopy.
Results: We found that the particles consisted of a bilayer membrane and a fluid-like interior. Flow cytometry
and interferometric light microscopy measurements showed that the majority of the particles were
nano-sized. Dynamic light scattering and interferometric light microscopy measurements agreed well with the
determined average hydrodynamic radius of the particles Rh (between 140 and 180 nm) while their number
densities were in the range between 10^13 and 10^14/mL indicating that hybridosomes present about 2/3 of the
mixture, excluding solvent and other small molecules.
Discussion: Simple and low-cost preparation method, non-demanding saving process and efficient
formation procedure suggest that large scale production of hybridosomes from lipids and spruce needle
homogenate is feasible.
PB  - Serbian Society for Extracellular Vesicles (SrbEVs)
PB  - Austrian Society for Extracellular Vesicles (ASEV)
PB  - Hungarian Society for Extracellular Vesicles (HSEV)
PB  - Serbian Society for Extracellular Vesicles (SrbEVs)
C3  - Small New World 2.0
T1  - Hybridosomes from spruce needle homogenate
EP  - 85
SP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2265
ER  - 

@conference{
author = "Spasovski, Vesna and Romolo, Anna and Kisovec, Matic and Zagorc, Urška and Arrigler, Vesna and Arko, Matevž and Bedina Zavec, Apolonija and Iglič, Aleš and Kogej, Ksenija and Kralj-Iglič, Veronika",
year = "2023",
abstract = "Introduction: Being of compatible structure with biomembranes, lipid–based nanoparticles are considered
as convenient platforms for drug delivery systems. In the proposed work we considered formation of lipid
nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called
hybridosomes). We formed hybridosomes by mixing appropriate amounts of lecithin, supernatant of isolation
of extracellular particles from spruce needle homogenate and glycerol.
Methods: We visualized hybridosomes by light microscopy and cryogenic transmission electron microscopy
and assessed them by flow cytometry, dynamic light scattering, ultraviolet–visual spectroscopy and
interferometric microscopy.
Results: We found that the particles consisted of a bilayer membrane and a fluid-like interior. Flow cytometry
and interferometric light microscopy measurements showed that the majority of the particles were
nano-sized. Dynamic light scattering and interferometric light microscopy measurements agreed well with the
determined average hydrodynamic radius of the particles Rh (between 140 and 180 nm) while their number
densities were in the range between 10^13 and 10^14/mL indicating that hybridosomes present about 2/3 of the
mixture, excluding solvent and other small molecules.
Discussion: Simple and low-cost preparation method, non-demanding saving process and efficient
formation procedure suggest that large scale production of hybridosomes from lipids and spruce needle
homogenate is feasible.",
publisher = "Serbian Society for Extracellular Vesicles (SrbEVs), Austrian Society for Extracellular Vesicles (ASEV), Hungarian Society for Extracellular Vesicles (HSEV), Serbian Society for Extracellular Vesicles (SrbEVs)",
journal = "Small New World 2.0",
title = "Hybridosomes from spruce needle homogenate",
pages = "85-85",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2265"
}

Spasovski, V., Romolo, A., Kisovec, M., Zagorc, U., Arrigler, V., Arko, M., Bedina Zavec, A., Iglič, A., Kogej, K.,& Kralj-Iglič, V.. (2023). Hybridosomes from spruce needle homogenate. in Small New World 2.0
Serbian Society for Extracellular Vesicles (SrbEVs)., 85-85.
https://hdl.handle.net/21.15107/rcub_imagine_2265

Spasovski V, Romolo A, Kisovec M, Zagorc U, Arrigler V, Arko M, Bedina Zavec A, Iglič A, Kogej K, Kralj-Iglič V. Hybridosomes from spruce needle homogenate. in Small New World 2.0. 2023;:85-85.
https://hdl.handle.net/21.15107/rcub_imagine_2265 .

Spasovski, Vesna, Romolo, Anna, Kisovec, Matic, Zagorc, Urška, Arrigler, Vesna, Arko, Matevž, Bedina Zavec, Apolonija, Iglič, Aleš, Kogej, Ksenija, Kralj-Iglič, Veronika, "Hybridosomes from spruce needle homogenate" in Small New World 2.0 (2023):85-85,
https://hdl.handle.net/21.15107/rcub_imagine_2265 .

TY  - CONF
AU  - Spasovski, Vesna
AU  - Romolo, Anna
AU  - Kisovec, Matic
AU  - Zagorc, Urška
AU  - Arrigler, Vesna
AU  - Arko, Matevž
AU  - Bedina Zavec, Apolonija
AU  - Iglič, Aleš
AU  - Kogej, Ksenija
AU  - Kralj-Iglič, Veronika
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2264
AB  - Introduction: Being of compatible structure with biomembranes, lipid–based nanoparticles are considered
as convenient platforms for drug delivery systems. In the proposed work we considered formation of lipid
nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called
hybridosomes). We formed hybridosomes by mixing appropriate amounts of lecithin, supernatant of isolation
of extracellular particles from spruce needle homogenate and glycerol.
Methods: We visualized hybridosomes by light microscopy and cryogenic transmission electron microscopy
and assessed them by flow cytometry, dynamic light scattering, ultraviolet–visual spectroscopy and
interferometric microscopy.
Results: We found that the particles consisted of a bilayer membrane and a fluid-like interior. Flow cytometry
and interferometric light microscopy measurements showed that the majority of the particles were
nano-sized. Dynamic light scattering and interferometric light microscopy measurements agreed well with the
determined average hydrodynamic radius of the particles Rh (between 140 and 180 nm) while their number
densities were in the range between 10^13 and 10^14/mL indicating that hybridosomes present about 2/3 of the
mixture, excluding solvent and other small molecules.
Discussion: Simple and low-cost preparation method, non-demanding saving process and efficient
formation procedure suggest that large scale production of hybridosomes from lipids and spruce needle
homogenate is feasible.
PB  - Serbian Society for Extracellular Vesicles (SrbEVs)
PB  - Austrian Society for Extracellular Vesicles (ASEV)
PB  - Hungarian Society for Extracellular Vesicles (HSEV)
PB  - Slovenian Network for Extracellular Vesicles (SiN-EV)
C3  - Small New World 2.0
T1  - Hybridosomes from spruce needle homogenate
EP  - 27
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2264
ER  - 

@conference{
author = "Spasovski, Vesna and Romolo, Anna and Kisovec, Matic and Zagorc, Urška and Arrigler, Vesna and Arko, Matevž and Bedina Zavec, Apolonija and Iglič, Aleš and Kogej, Ksenija and Kralj-Iglič, Veronika",
year = "2023",
abstract = "Introduction: Being of compatible structure with biomembranes, lipid–based nanoparticles are considered
as convenient platforms for drug delivery systems. In the proposed work we considered formation of lipid
nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called
hybridosomes). We formed hybridosomes by mixing appropriate amounts of lecithin, supernatant of isolation
of extracellular particles from spruce needle homogenate and glycerol.
Methods: We visualized hybridosomes by light microscopy and cryogenic transmission electron microscopy
and assessed them by flow cytometry, dynamic light scattering, ultraviolet–visual spectroscopy and
interferometric microscopy.
Results: We found that the particles consisted of a bilayer membrane and a fluid-like interior. Flow cytometry
and interferometric light microscopy measurements showed that the majority of the particles were
nano-sized. Dynamic light scattering and interferometric light microscopy measurements agreed well with the
determined average hydrodynamic radius of the particles Rh (between 140 and 180 nm) while their number
densities were in the range between 10^13 and 10^14/mL indicating that hybridosomes present about 2/3 of the
mixture, excluding solvent and other small molecules.
Discussion: Simple and low-cost preparation method, non-demanding saving process and efficient
formation procedure suggest that large scale production of hybridosomes from lipids and spruce needle
homogenate is feasible.",
publisher = "Serbian Society for Extracellular Vesicles (SrbEVs), Austrian Society for Extracellular Vesicles (ASEV), Hungarian Society for Extracellular Vesicles (HSEV), Slovenian Network for Extracellular Vesicles (SiN-EV)",
journal = "Small New World 2.0",
title = "Hybridosomes from spruce needle homogenate",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2264"
}

Spasovski, V., Romolo, A., Kisovec, M., Zagorc, U., Arrigler, V., Arko, M., Bedina Zavec, A., Iglič, A., Kogej, K.,& Kralj-Iglič, V.. (2023). Hybridosomes from spruce needle homogenate. in Small New World 2.0
Serbian Society for Extracellular Vesicles (SrbEVs)., 27-27.
https://hdl.handle.net/21.15107/rcub_imagine_2264

Spasovski V, Romolo A, Kisovec M, Zagorc U, Arrigler V, Arko M, Bedina Zavec A, Iglič A, Kogej K, Kralj-Iglič V. Hybridosomes from spruce needle homogenate. in Small New World 2.0. 2023;:27-27.
https://hdl.handle.net/21.15107/rcub_imagine_2264 .

Spasovski, Vesna, Romolo, Anna, Kisovec, Matic, Zagorc, Urška, Arrigler, Vesna, Arko, Matevž, Bedina Zavec, Apolonija, Iglič, Aleš, Kogej, Ksenija, Kralj-Iglič, Veronika, "Hybridosomes from spruce needle homogenate" in Small New World 2.0 (2023):27-27,
https://hdl.handle.net/21.15107/rcub_imagine_2264 .

TY  - JOUR
AU  - Troha, Kaja
AU  - Vozel, Domen
AU  - Arko, Matevž
AU  - Bedina Zavec, Apolonija
AU  - Dolinar, Drago
AU  - Hočevar, Matej
AU  - Jan, Zala
AU  - Kisovec, Matic
AU  - Kocjančič, Boštjan
AU  - Pađen, Ljubiša
AU  - Pajnič, Manca
AU  - Penič, Samo
AU  - Romolo, Anna
AU  - Repar, Neža
AU  - Spasovski, Vesna
AU  - Steiner, Nejc
AU  - Šuštar, Vid
AU  - Iglič, Aleš
AU  - Drobne, Damjana
AU  - Kogej, Ksenija
AU  - Battelino, Saba
AU  - Kralj-Iglič, Veronika
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2087
AB  - The preparation of autologous platelet and extracellular vesicle-rich plasma (PVRP) has been explored in many medical fields with the aim to benefit from its healing potential. In parallel, efforts are being invested to understand the function and dynamics of PVRP that is complex in its composition and interactions. Some clinical evidence reveals beneficial effects of PVRP, while some report that there were no effects. To optimize the preparation methods, functions and mechanisms of PVRP, its constituents should be better understood. With the intention to promote further studies of autologous therapeutic PVRP, we performed a review on some topics regarding PVRP composition, harvesting, assessment and preservation, and also on clinical experience following PVRP application in humans and animals. Besides the acknowledged actions of platelets, leukocytes and different molecules, we focus on extracellular vesicles that were found abundant in PVRP.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review
IS  - 4
SP  - 3420
VL  - 24
DO  - 10.3390/ijms24043420
ER  - 

@article{
author = "Troha, Kaja and Vozel, Domen and Arko, Matevž and Bedina Zavec, Apolonija and Dolinar, Drago and Hočevar, Matej and Jan, Zala and Kisovec, Matic and Kocjančič, Boštjan and Pađen, Ljubiša and Pajnič, Manca and Penič, Samo and Romolo, Anna and Repar, Neža and Spasovski, Vesna and Steiner, Nejc and Šuštar, Vid and Iglič, Aleš and Drobne, Damjana and Kogej, Ksenija and Battelino, Saba and Kralj-Iglič, Veronika",
year = "2023",
abstract = "The preparation of autologous platelet and extracellular vesicle-rich plasma (PVRP) has been explored in many medical fields with the aim to benefit from its healing potential. In parallel, efforts are being invested to understand the function and dynamics of PVRP that is complex in its composition and interactions. Some clinical evidence reveals beneficial effects of PVRP, while some report that there were no effects. To optimize the preparation methods, functions and mechanisms of PVRP, its constituents should be better understood. With the intention to promote further studies of autologous therapeutic PVRP, we performed a review on some topics regarding PVRP composition, harvesting, assessment and preservation, and also on clinical experience following PVRP application in humans and animals. Besides the acknowledged actions of platelets, leukocytes and different molecules, we focus on extracellular vesicles that were found abundant in PVRP.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review",
number = "4",
pages = "3420",
volume = "24",
doi = "10.3390/ijms24043420"
}

Troha, K., Vozel, D., Arko, M., Bedina Zavec, A., Dolinar, D., Hočevar, M., Jan, Z., Kisovec, M., Kocjančič, B., Pađen, L., Pajnič, M., Penič, S., Romolo, A., Repar, N., Spasovski, V., Steiner, N., Šuštar, V., Iglič, A., Drobne, D., Kogej, K., Battelino, S.,& Kralj-Iglič, V.. (2023). Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review. in International Journal of Molecular Sciences
MDPI., 24(4), 3420.
https://doi.org/10.3390/ijms24043420

Troha K, Vozel D, Arko M, Bedina Zavec A, Dolinar D, Hočevar M, Jan Z, Kisovec M, Kocjančič B, Pađen L, Pajnič M, Penič S, Romolo A, Repar N, Spasovski V, Steiner N, Šuštar V, Iglič A, Drobne D, Kogej K, Battelino S, Kralj-Iglič V. Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review. in International Journal of Molecular Sciences. 2023;24(4):3420.
doi:10.3390/ijms24043420 .

Troha, Kaja, Vozel, Domen, Arko, Matevž, Bedina Zavec, Apolonija, Dolinar, Drago, Hočevar, Matej, Jan, Zala, Kisovec, Matic, Kocjančič, Boštjan, Pađen, Ljubiša, Pajnič, Manca, Penič, Samo, Romolo, Anna, Repar, Neža, Spasovski, Vesna, Steiner, Nejc, Šuštar, Vid, Iglič, Aleš, Drobne, Damjana, Kogej, Ksenija, Battelino, Saba, Kralj-Iglič, Veronika, "Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review" in International Journal of Molecular Sciences, 24, no. 4 (2023):3420,
https://doi.org/10.3390/ijms24043420 . .

TY  - JOUR
AU  - Romolo, Anna
AU  - Jan, Zala
AU  - Bedina Zavec, Apolonija
AU  - Kisovec, Matic
AU  - Arrigler, Vesna
AU  - Spasovski, Vesna
AU  - Podobnik, Marjetka
AU  - Iglič, Aleš
AU  - Pocsfalvi, Gabriella
AU  - Kogej, Ksenija
AU  - Kralj-Iglič, Veronika
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2090
AB  - Small particles in natural sources are a subject of interest for their potential role in intercellular, inter-organism, and inter-species interactions, but their harvesting and assessment present a challenge due to their small size and transient identity. We applied a recently developed interferometric light microscopy (ILM) to assess the number density and hydrodynamic radius (Rh) of isolated small cellular particles (SCPs) from blood preparations (plasma and washed erythrocytes) (B), spruce needle homogenate (S), suspension of flagellae of microalgae Tetraselmis chuii (T), conditioned culture media of microalgae Phaeodactylum tricornutum (P), and liposomes (L). The aliquots were also assessed by flow cytometry (FCM), dynamic light scattering (DLS), ultraviolet-visible spectrometry (UV-vis), and imaging by cryogenic transmission electron microscopy (cryo-TEM). In Rh, ILM showed agreement with DLS within the measurement error in 10 out of 13 samples and was the only method used here that yielded particle density. Cryo-TEM revealed that representative SCPs from Tetraselmis chuii flagella (T) did not have a globular shape, so the interpretation by Rh of the batch methods was biased. Cryo-TEM showed the presence of thin filaments in isolates from Phaeodactylum tricornutum conditioned culture media (P), which provides an explanation for the considerably larger Rh obtained by batch methods than the sizes of particles observed by cryo-TEM images. ILM proved convenient for assessment of number density and Rh of SCPs in blood preparations (e.g., plasma); therefore, its use in population and clinical studies is indicated.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Assessment of Small Cellular Particles from Four Different Natural Sources and Liposomes by Interferometric Light Microscopy
IS  - 24
SP  - 15801
VL  - 23
DO  - 10.3390/ijms232415801
ER  - 

@article{
author = "Romolo, Anna and Jan, Zala and Bedina Zavec, Apolonija and Kisovec, Matic and Arrigler, Vesna and Spasovski, Vesna and Podobnik, Marjetka and Iglič, Aleš and Pocsfalvi, Gabriella and Kogej, Ksenija and Kralj-Iglič, Veronika",
year = "2022",
abstract = "Small particles in natural sources are a subject of interest for their potential role in intercellular, inter-organism, and inter-species interactions, but their harvesting and assessment present a challenge due to their small size and transient identity. We applied a recently developed interferometric light microscopy (ILM) to assess the number density and hydrodynamic radius (Rh) of isolated small cellular particles (SCPs) from blood preparations (plasma and washed erythrocytes) (B), spruce needle homogenate (S), suspension of flagellae of microalgae Tetraselmis chuii (T), conditioned culture media of microalgae Phaeodactylum tricornutum (P), and liposomes (L). The aliquots were also assessed by flow cytometry (FCM), dynamic light scattering (DLS), ultraviolet-visible spectrometry (UV-vis), and imaging by cryogenic transmission electron microscopy (cryo-TEM). In Rh, ILM showed agreement with DLS within the measurement error in 10 out of 13 samples and was the only method used here that yielded particle density. Cryo-TEM revealed that representative SCPs from Tetraselmis chuii flagella (T) did not have a globular shape, so the interpretation by Rh of the batch methods was biased. Cryo-TEM showed the presence of thin filaments in isolates from Phaeodactylum tricornutum conditioned culture media (P), which provides an explanation for the considerably larger Rh obtained by batch methods than the sizes of particles observed by cryo-TEM images. ILM proved convenient for assessment of number density and Rh of SCPs in blood preparations (e.g., plasma); therefore, its use in population and clinical studies is indicated.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Assessment of Small Cellular Particles from Four Different Natural Sources and Liposomes by Interferometric Light Microscopy",
number = "24",
pages = "15801",
volume = "23",
doi = "10.3390/ijms232415801"
}

Romolo, A., Jan, Z., Bedina Zavec, A., Kisovec, M., Arrigler, V., Spasovski, V., Podobnik, M., Iglič, A., Pocsfalvi, G., Kogej, K.,& Kralj-Iglič, V.. (2022). Assessment of Small Cellular Particles from Four Different Natural Sources and Liposomes by Interferometric Light Microscopy. in International Journal of Molecular Sciences
MDPI., 23(24), 15801.
https://doi.org/10.3390/ijms232415801

Romolo A, Jan Z, Bedina Zavec A, Kisovec M, Arrigler V, Spasovski V, Podobnik M, Iglič A, Pocsfalvi G, Kogej K, Kralj-Iglič V. Assessment of Small Cellular Particles from Four Different Natural Sources and Liposomes by Interferometric Light Microscopy. in International Journal of Molecular Sciences. 2022;23(24):15801.
doi:10.3390/ijms232415801 .

Romolo, Anna, Jan, Zala, Bedina Zavec, Apolonija, Kisovec, Matic, Arrigler, Vesna, Spasovski, Vesna, Podobnik, Marjetka, Iglič, Aleš, Pocsfalvi, Gabriella, Kogej, Ksenija, Kralj-Iglič, Veronika, "Assessment of Small Cellular Particles from Four Different Natural Sources and Liposomes by Interferometric Light Microscopy" in International Journal of Molecular Sciences, 23, no. 24 (2022):15801,
https://doi.org/10.3390/ijms232415801 . .